Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A26 F57 I1 R1	119	93.8	3687158	95.5%	3521235	127.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	3,723,067	1 bp→AT	intergenic (‑181/‑120)	U712_19190 ← / → U712_19195	PTS system sucrose‑specific EIIBC component/putative transporter ywcJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,723,066	1	.	A	94.4%	357.1 / 12.4	108	intergenic (‑180/‑121)	U712_19190/U712_19195	PTS system sucrose‑specific EIIBC component/putative transporter ywcJ
Reads supporting (aligned to +/- strand): ref base . (4/2); new base A (38/64); total (42/66)
Fisher's exact test for biased strand distribution p-value = 2.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	CP006881	3,723,067	0	G	T	96.3%	366.5 / 2.4	108	intergenic (‑181/‑120)	U712_19190/U712_19195	PTS system sucrose‑specific EIIBC component/putative transporter ywcJ
Reads supporting (aligned to +/- strand): ref base G (0/0); major base T (39/65); minor base A (3/1); total (42/66)
Fisher's exact test for biased strand distribution p-value = 2.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01

Only 100 of 108 total aligned reads displayed.

CGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTGAGAATACGCTTTCATCATAAATTATGATAGCGTTTTCGTCAAC‑‑T‑GTT‑T‑TTTAGTTAAATAGTTTGATAAAACTTTTAGACAAAAATTCGTAAAAACTATTTGTGAAAAACTTCACAATATCGTGCTATACTATGCTCAACCATCAAAGAAAGCAGGAAAAAACAATGGAAAC  >  CP006881/3722935‑3723194

||

cGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑T‑Att‑t‑ttt                                                                                                                           >  3:56953/1‑139 (MQ=255)

        ggACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTaaa                                                                                                                    <  1:272774/139‑1 (MQ=255)

        ggACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTaaa                                                                                                                    <  4:173960/139‑1 (MQ=255)

           cacaGCTATCCTTATTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACCATCCCGCATATTCAGATTAACAATACGCTTTCATCATAAATAATGATAGCGTTTTCGTCTAC‑‑TATTT‑T‑TTTAGTTATATAg                                                                                                                 >  4:389183/1‑139 (MQ=255)

           cacaGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTAAATAg                                                                                                                 >  7:278891/1‑139 (MQ=255)

           cacaGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTAAATAg                                                                                                                 >  2:333121/1‑139 (MQ=255)

           cacaGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATCATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTAAATAg                                                                                                                 >  6:7275/1‑139 (MQ=255)

           cacaGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGATTTCTTCTTAAATCATGATAGCGTTTTCGTAAAC‑‑TATTT‑T‑TTTATTTAAATAg                                                                                                                 >  8:320698/1‑139 (MQ=255)

            acaGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTAAATAGt                                                                                                                <  1:226258/139‑1 (MQ=255)

            acaGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTAAATAGt                                                                                                                <  7:87968/139‑1 (MQ=255)

            acaGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTAAATAGt                                                                                                                >  7:185643/1‑139 (MQ=255)

            acaGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTAAATAGt                                                                                                                >  5:26115/1‑139 (MQ=255)

             caGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTAAATAGtt                                                                                                               <  3:191937/139‑1 (MQ=255)

             caGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTAAATAGtt                                                                                                               <  2:39457/139‑1 (MQ=255)

              aGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTa                                                                                                                      >  4:31213/1‑131 (MQ=255)

              aGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTa                                                                                                                      <  3:31213/131‑1 (MQ=255)

              aGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTAAATAGttt                                                                                                              >  8:262309/1‑139 (MQ=255)

              aGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTAGCATGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTAAATAGttt                                                                                                              >  8:254983/1‑139 (MQ=255)

                   tCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTAAATAGTTTGATat                                                                                                         <  2:279471/139‑2 (MQ=255)

                   tCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTAAATAGTTTGATat                                                                                                         <  3:217091/139‑2 (MQ=255)

                   tCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTAAATAGTTTGATa                                                                                                          >  5:151080/1‑138 (MQ=255)

                   tCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTAAATAGTTTGATa                                                                                                          >  7:238406/1‑138 (MQ=255)

                   accTTTTACATTTACGCAAATTTTAGGTCTTGCATGCTTTACCAGTCACAATCCCGCATATTCAGATTAAGAATACGCTAACATAATAAATAATTATAGCGTATTCGTCAAC‑‑T‑ATTAA‑TTTAGATAAATATATTGATa                                                                                                          >  4:254077/2‑138 (MQ=255)

                        tttCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTAAATAGTTTGATATAAcat                                                                                                    >  3:114113/1‑137 (MQ=255)

                         ttCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTAAATAGTTTGATATAAcat                                                                                                    <  4:450647/138‑3 (MQ=255)

                             tttACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTAAATAGTTTGATATAACATGTAGa                                                                                               >  8:298773/1‑139 (MQ=255)

                                          tAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTa                                                                                   <  1:94151/138‑1 (MQ=255)

                                                ttGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTaaaaatt                                                                             >  3:389541/1‑136 (MQ=255)

                                                  gCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTaaatattaa                                                                           <  4:56953/138‑7 (MQ=255)

                                                    cTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATtg                                                                        <  1:214423/139‑1 (MQ=255)

                                                        tttACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGa                                                                     >  5:116764/1‑138 (MQ=255)

                                                            ccAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                                                                 <  8:238406/138‑3 (MQ=255)

                                                            ccAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                                                                 <  6:151080/138‑3 (MQ=255)

                                                               gTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACtt                                                              >  6:128863/1‑138 (MQ=255)

                                                                tCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTca                                                            <  4:114113/139‑1 (MQ=255)

                                                                tCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTca                                                            <  6:335179/139‑1 (MQ=255)

                                                                 cacaATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                                                                 >  2:116741/1‑131 (MQ=255)

                                                                 cacaATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                                                                 <  1:116741/133‑3 (MQ=255)

                                                                 cacaATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTca                                                            >  6:198536/1‑138 (MQ=255)

                                                                  acaATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTcaca                                                          <  6:26115/139‑1 (MQ=255)

                                                                     ataccGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTAAAAAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                       <  8:301596/136‑1 (MQ=255)

                                                                     aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTGGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                       <  1:411585/139‑1 (MQ=255)

                                                                     aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                       <  6:73875/139‑1 (MQ=255)

                                                                     aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                       <  5:297080/139‑1 (MQ=255)

                                                                     aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                       <  7:14325/139‑1 (MQ=255)

                                                                     aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                       <  7:184119/139‑1 (MQ=255)

                                                                     aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                       <  7:262309/139‑1 (MQ=255)

                                                                     aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                       <  5:198536/139‑1 (MQ=255)

                                                                     aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                       <  5:137632/139‑1 (MQ=255)

                                                                     aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                       <  3:299924/139‑1 (MQ=255)

                                                                     aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                       <  4:347627/139‑1 (MQ=255)

                                                                     aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                       <  4:322200/139‑1 (MQ=255)

                                                                     aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                       <  2:239838/139‑1 (MQ=255)

                                                                     aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑T‑ATTAT‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                       <  5:423411/139‑1 (MQ=255)

                                                                     aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGATTTCGTCAAC‑‑TATTT‑T‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                       <  5:389963/139‑1 (MQ=255)

                                                                     aTCCAGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                       <  7:196545/139‑1 (MQ=255)

                                                                      tCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑TTT‑T‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                       <  2:231431/139‑1 (MQ=255)

                                                                      tCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAatat                                                      <  8:29806/139‑1 (MQ=255)

                                                                        ccGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATc                                                     <  4:389541/138‑1 (MQ=255)

                                                                        ccGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATc                                                     <  7:254983/138‑1 (MQ=255)

                                                                         cGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                       <  3:228698/135‑1 (MQ=255)

                                                                         cGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                       <  8:110714/135‑1 (MQ=255)

                                                                         cGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                       >  7:110714/1‑135 (MQ=255)

                                                                         cGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                       >  4:228698/1‑135 (MQ=255)

                                                                          gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCATC‑‑TATTT‑T‑TTTAGTTAAATAGTTTGATATAACATGTAGAGAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                                                  <  3:254077/139‑1 (MQ=255)

                                                                          gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                                                  <  3:15643/139‑1 (MQ=255)

                                                                          gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                                                  <  4:324505/139‑1 (MQ=255)

                                                                          gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                                                  <  8:278891/139‑1 (MQ=255)

                                                                          gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                                                  <  1:333121/139‑1 (MQ=255)

                                                                          gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTAAAC‑‑TATTT‑T‑TTTAGTTAAATAGTTTGATATAACTTGTAGTCAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCTTg                                                  <  3:389183/139‑1 (MQ=255)

                                                                          gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATCATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                                                  <  5:7275/139‑1 (MQ=255)

                                                                           cgtATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTTGTTAAATAGTTTGATATACCATGTAGCCAAAAATTCGTAAAAATTAATTGTGAAAGACTTCACATTATCGTGc                                                 >  2:183430/3‑139 (MQ=255)

                                                                              aTTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTaa                                                                           <  6:242644/110‑2 (MQ=255)

                                                                              aTTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTaa                                                                           >  5:242644/1‑109 (MQ=255)

                                                                              aTTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCta                                               <  3:347793/138‑1 (MQ=255)

                                                                               ttCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCtata                                             <  8:185643/139‑1 (MQ=255)

                                                                               ttCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCtata                                             <  5:374105/139‑1 (MQ=255)

                                                                               ttCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCtata                                             <  5:368049/139‑1 (MQ=255)

                                                                                    aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                       >  7:52470/1‑124 (MQ=255)

                                                                                       aagaATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCt                                      <  3:435669/137‑1 (MQ=255)

                                                                                         gaATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCaa                                   <  2:375832/139‑1 (MQ=255)

                                                                                              cGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACA‑A‑TTT‑T‑TTTAGTTAAATAGTTTGATATAACATGTAGCCAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCACAATCAt                               >  4:90630/1‑138 (MQ=255)

                                                                                              cGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAatat                                                      >  2:64815/1‑115 (MQ=255)

                                                                                              cGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAatat                                                      <  1:64815/115‑1 (MQ=255)

                                                                                              cGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                       <  4:393415/114‑1 (MQ=255)

                                                                                              cGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                       <  1:262635/114‑1 (MQ=255)

                                                                                              cGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                       >  3:393415/1‑114 (MQ=255)

                                                                                              cGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                       >  2:262635/1‑114 (MQ=255)

                                                                                              cGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCAt                               >  6:311636/1‑138 (MQ=255)

                                                                                              cGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCAt                               >  4:361546/1‑138 (MQ=255)

                                                                                              cGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCAt                               >  5:260316/1‑138 (MQ=255)

                                                                                              cGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCAt                               >  5:289973/1‑138 (MQ=255)

                                                                                                   tcatcaTAAATCATGATAGCGTTTTCGTCAAC‑‑T‑ATT‑TATTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGaaag                          >  3:334062/1‑138 (MQ=255)

                                                                                                    catcatAAATCATGATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGaaaga                         >  7:188021/1‑138 (MQ=255)

                                                                                                    catcatAAATCATGATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGaaaga                         >  1:96776/1‑138 (MQ=255)

                                                                                                                tGATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGaaaaga            >  4:163334/1‑137 (MQ=255)

                                                                                                                tGATAGCGTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGaaaag             >  2:247400/1‑137 (MQ=255)

                                                                                                                       gTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGa     <  5:128863/139‑1 (MQ=255)

                                                                                                                       gTTTTCGTCAAC‑‑TATTT‑T‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGa     <  1:286639/139‑1 (MQ=255)

                                                                                                                          ttCGTCAAC‑‑TATTT‑T‑TTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAAc  >  7:170636/1‑139 (MQ=255)

||

CGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTGAGAATACGCTTTCATCATAAATTATGATAGCGTTTTCGTCAAC‑‑T‑GTT‑T‑TTTAGTTAAATAGTTTGATAAAACTTTTAGACAAAAATTCGTAAAAACTATTTGTGAAAAACTTCACAATATCGTGCTATACTATGCTCAACCATCAAAGAAAGCAGGAAAAAACAATGGAAAC  >  CP006881/3722935‑3723194

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A