Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A28 F56 I1 R1	120	79.0	2973134	95.3%	2833396	126.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	3,723,067	1 bp→AT	intergenic (‑181/‑120)	U712_19190 ← / → U712_19195	PTS system sucrose‑specific EIIBC component/putative transporter ywcJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,723,066	1	.	A	96.8%	320.0 / 2.1	94	intergenic (‑180/‑121)	U712_19190/U712_19195	PTS system sucrose‑specific EIIBC component/putative transporter ywcJ
Reads supporting (aligned to +/- strand): ref base . (3/0); new base A (38/53); total (41/53)
Fisher's exact test for biased strand distribution p-value = 7.95e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	CP006881	3,723,067	0	G	T	96.8%	317.3 / ‑2.2	94	intergenic (‑181/‑120)	U712_19190/U712_19195	PTS system sucrose‑specific EIIBC component/putative transporter ywcJ
Reads supporting (aligned to +/- strand): ref base G (0/0); major base T (38/53); minor base A (3/0); total (41/53)
Fisher's exact test for biased strand distribution p-value = 7.95e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTGAGAATACGCTTTCATCATAAATTATGATAGCGTTTTCGTCAACT‑GT‑T‑TTT‑TAGTTAAATAGTTTGATAAAACTTTTAGACAAAAATTCGTAAAAACTATTTGTGAAAAACTTCACAATATCGTGCTATACTATGCTCAACCATCAAAGAAAGCAGGAAAAAACAATGGAAACTCAAGC  >  CP006881/3722943‑3723200

||

ggACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTaaa                                                                                                                          <  5:357198/139‑1 (MQ=255)

ggACACAGCGATCCTTCTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTaaa                                                                                                                          <  6:356389/139‑1 (MQ=255)

   cacaGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGATTATTCAGATTAAGAATACGCTTTCATCATAAATCATTATAGCGTATTCGTCAACT‑AT‑TATTT‑TATTTAAATAg                                                                                                                       >  2:278359/1‑139 (MQ=255)

    acaGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGt                                                                                                                      <  2:360778/139‑1 (MQ=255)

    acaGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGt                                                                                                                      >  2:107999/1‑139 (MQ=255)

    acaGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGt                                                                                                                      >  5:364596/1‑139 (MQ=255)

    acaGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGt                                                                                                                      <  5:61796/139‑1 (MQ=255)

    acaGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGt                                                                                                                      >  5:83431/1‑139 (MQ=255)

    acaGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGt                                                                                                                      >  1:360778/1‑139 (MQ=255)

    acaGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGt                                                                                                                      <  4:327794/139‑1 (MQ=255)

    acaGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGt                                                                                                                      <  6:97374/139‑1 (MQ=255)

    acaGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGt                                                                                                                      >  3:327794/1‑139 (MQ=255)

    acaGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCACTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGt                                                                                                                      >  3:92503/1‑139 (MQ=255)

      aGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGttt                                                                                                                    >  8:100576/1‑139 (MQ=255)

      aGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGttt                                                                                                                    >  2:123563/1‑139 (MQ=255)

         gATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGAt                                                                                                                 <  8:281056/139‑1 (MQ=255)

          aTCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATa                                                                                                                >  4:45070/1‑139 (MQ=255)

           tgcTTTTTCATTTAAGCAAATTTTAGGTCTTGCCAGCTTTACCAGTCACAATGACGCTTATTAAGATTAAGAATACGCTTTCATCATACATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATat                                                                                                               <  2:145430/137‑2 (MQ=255)

           tCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATat                                                                                                               <  8:355930/139‑2 (MQ=255)

           tCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATa                                                                                                                >  2:69233/1‑138 (MQ=255)

                tttCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAAcat                                                                                                          >  4:164042/1‑137 (MQ=255)

                tttCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAAcat                                                                                                          >  7:341688/1‑137 (MQ=255)

                   cATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAAcatgt                                                                                                        <  4:355052/138‑5 (MQ=255)

                       tACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGt                                                                                                                      >  4:80598/1‑120 (MQ=255)

                       tACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGt                                                                                                                      <  3:80598/120‑1 (MQ=255)

                                        ttGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGtt                                                                                                                     >  1:75333/1‑104 (MQ=255)

                                        ttGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGtt                                                                                                                     <  2:75333/104‑1 (MQ=255)

                                          gCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATATTTTTATATAAAATGTAGACAAAAATTCGTAAAAATTaat                                                                                >  6:207002/1‑137 (MQ=255)

                                          gCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTaat                                                                                >  5:81845/1‑137 (MQ=255)

                                          gCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTaa                                                                                 <  6:132669/138‑2 (MQ=255)

                                          gCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTaa                                                                                 <  8:124217/138‑2 (MQ=255)

                                          gCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTaa                                                                                 <  1:123563/138‑2 (MQ=255)

                                          gCCTGCTTTACAAGTCACAATCCCGCTAATTCAGATTAAGAATACGCTTTCATCATAAATCATGATACCGTTTTCGTCAACT‑ATAT‑TAT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTaat                                                                                >  4:218361/1‑137 (MQ=255)

                                          gCCTGCGTTACCAGTCACAATCCCGCTTAATCAGAATAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAAATCGTaaaaataaa                                                                                 <  2:342391/138‑5 (MQ=255)

                                               cTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTTATATAACATGTAGACAAAAATTCGTAAAAATTAATtgtg                                                                            >  3:193839/1‑138 (MQ=255)

                                               cTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATtgtg                                                                            <  5:157871/138‑1 (MQ=255)

                                               cTTTACCAGTCACAATCCCGCATATTCAGATTAAGAATAAGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATtgtg                                                                            <  2:129027/138‑1 (MQ=255)

                                                  tACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaa                                                                          <  5:28321/137‑1 (MQ=255)

                                                  tACCAGTCACAATCCCGCATATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaa                                                                         <  6:28021/138‑1 (MQ=255)

                                                    ccAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                                                                       <  7:253747/138‑3 (MQ=255)

                                                       gTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACtt                                                                    >  8:304629/1‑138 (MQ=255)

                                                        tCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTca                                                                  <  5:255628/139‑1 (MQ=255)

                                                        tCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTca                                                                  <  4:352789/139‑1 (MQ=255)

                                                         cacaATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTca                                                                  >  1:134043/1‑138 (MQ=255)

                                                         cacaATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACT‑AT‑T‑TTTATAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATAGTGAAATACtttc                                                                  >  6:255628/1‑136 (MQ=255)

                                                          acaATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTcaca                                                                <  8:64825/139‑1 (MQ=255)

                                                             aTCCCGCTTATTCAGATTAAGACTACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                             <  6:338324/139‑1 (MQ=255)

                                                             aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                             <  7:100576/139‑1 (MQ=255)

                                                             aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                             <  6:83431/139‑1 (MQ=255)

                                                             aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                             <  3:164042/139‑1 (MQ=255)

                                                             aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                             <  1:127838/139‑1 (MQ=255)

                                                             aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                             <  1:165625/139‑1 (MQ=255)

                                                             aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                             <  7:310102/139‑1 (MQ=255)

                                                             aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                             <  1:107999/139‑1 (MQ=255)

                                                             aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                             <  7:270484/139‑1 (MQ=255)

                                                             aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTATTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTATACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                             <  5:264657/139‑1 (MQ=255)

                                                             aTCCCGCTTATTCAGATTAAGAATACGCATTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTATATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                             <  3:105568/139‑1 (MQ=255)

                                                             aTCCCACTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                             <  4:92503/139‑1 (MQ=255)

                                                              tCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGAGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATTTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                             >  1:57595/1‑138 (MQ=255)

                                                                ccGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATc                                                           <  2:305959/138‑1 (MQ=255)

                                                                cagCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTAGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATc                                                           <  1:278359/136‑1 (MQ=255)

                                                                 cGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TATTTAAATATTTTGATATAACATTTAGACAAAAATACGTAAAAATTAATTGTGATATACTTCACAataaggt                                                         >  3:257578/1‑135 (MQ=255)

                                                                  gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                             <  2:263562/134‑1 (MQ=255)

                                                                  gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                             <  2:310993/134‑1 (MQ=255)

                                                                  gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                             >  1:310993/1‑134 (MQ=255)

                                                                  gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                             >  1:263562/1‑134 (MQ=255)

                                                                  gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                                                        <  7:304629/139‑1 (MQ=255)

                                                                  gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                                                        <  6:364596/139‑1 (MQ=255)

                                                                  gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                                                        <  8:341688/139‑1 (MQ=255)

                                                                  gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGGTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                                                        <  6:47472/139‑1 (MQ=255)

                                                                   attATTCATATTAAGAATAATATTTCATCATTAATAATGATAGAGTTTTAGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAAAATTTAGTCAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                                                        <  2:134043/137‑1 (MQ=255)

                                                                      aTTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCt                                                      <  1:69233/137‑1 (MQ=255)

                                                                       ttCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCtata                                                   <  4:48731/139‑1 (MQ=255)

                                                                          aGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACt                                                 <  6:81845/138‑1 (MQ=255)

                                                                            aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                                                                       >  8:199013/1‑112 (MQ=255)

                                                                            aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                                                                       <  7:194478/114‑3 (MQ=255)

                                                                            aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                                                                       <  7:199013/114‑3 (MQ=255)

                                                                            aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                                                                       >  8:194478/1‑112 (MQ=255)

                                                                            aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                             >  1:17858/1‑124 (MQ=255)

                                                                            aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                             <  2:17858/124‑1 (MQ=255)

                                                                            aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATg                                              >  8:161162/1‑139 (MQ=255)

                                                                            aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATg                                              >  1:87316/1‑139 (MQ=255)

                                                                             ttaagaATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGc                                             >  3:70744/4‑139 (MQ=255)

                                                                                      cGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCAt                                     >  1:274451/1‑138 (MQ=255)

                                                                                      cGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCAt                                     >  8:138125/1‑138 (MQ=255)

                                                                                             atcatAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGaaagaa                              <  6:328454/138‑1 (MQ=255)

                                                                                                 tAAATCATGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTATTTAGTTTGATATAACATGTAGACAAAAATTCTTAAAAATTAATTGTGATATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAg                         >  2:97765/1‑139 (MQ=255)

                                                                                                       aTGATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGaa                      >  6:231667/1‑136 (MQ=255)

                                                                                                         gATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTATACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATc                                                           >  7:250073/1‑97 (MQ=255)

                                                                                                         gATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATc                                                           <  8:250073/97‑1 (MQ=255)

                                                                                                         gATAGCGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTTTACTATGCTCAATCATGAAAGAAAGCAGGaaaaga                  >  2:186308/1‑136 (MQ=255)

                                                                                                              cGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATg             >  1:49510/1‑138 (MQ=255)

                                                                                                              cGTTTTCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATg             >  5:104385/1‑138 (MQ=255)

                                                                                                                   tCGTCAACTATT‑T‑TTT‑TAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACt       >  1:364571/1‑139 (MQ=255)

                                                                                                                        aactatt‑t‑ttt‑tAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGc  <  2:87316/133‑1 (MQ=255)

||

GGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTGAGAATACGCTTTCATCATAAATTATGATAGCGTTTTCGTCAACT‑GT‑T‑TTT‑TAGTTAAATAGTTTGATAAAACTTTTAGACAAAAATTCGTAAAAACTATTTGTGAAAAACTTCACAATATCGTGCTATACTATGCTCAACCATCAAAGAAAGCAGGAAAAAACAATGGAAACTCAAGC  >  CP006881/3722943‑3723200

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A