Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A14 F65 I0 R1 86 259.7 8019954 97.7% 7835495 136.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,877,923 A→G 100% Q24R (CAG→CGG)  rnpA → protein C5 component of RNase P

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,877,9230AG99.1% 1099.0 / ‑0.5 316Q24R (CAG→CGG) rnpAprotein C5 component of RNase P
Reads supporting (aligned to +/- strand):  ref base A (1/2);  new base G (187/125);  total (189/127)
Fisher's exact test for biased strand distribution p-value = 5.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 329 total aligned reads displayed.

CTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGAC  >  CP009273/3877786‑3878058
                                                                                                                                         |                                                                                                                                       
cTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCagcggc                                                                                                                                       <  1:791508/140‑1 (MQ=255)
cTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCagcggc                                                                                                                                       <  1:885969/140‑1 (MQ=255)
cTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCagcggc                                                                                                                                       <  2:2108938/140‑1 (MQ=255)
cTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCGTGTTAACTCCCAGTCAATTCACATTCGTCTTCCagcggc                                                                                                                                       >  1:2137998/1‑140 (MQ=255)
  ggCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCGGCca                                                                                                                                     >  2:2447265/1‑140 (MQ=255)
     aCGTCGTCTTGCTAAAGGCCGAGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCTCGCATTTCCCAGGGTGTTACGATTGTTACCTCCCAGTCAATTCACATTCGTCTTCCAGCGGCcacac                                                                                                                                  <  2:943307/140‑2 (MQ=255)
       gtcgtcgtGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTTACATTCGTCTTCCAGCGGCCACAACg                                                                                                                                >  1:2578006/1‑140 (MQ=255)
        tcgtcgtGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCGGCCACAACgg                                                                                                                               >  1:3937611/1‑140 (MQ=255)
        tcgtcgtGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCGGCCACAACgg                                                                                                                               >  1:1719333/1‑140 (MQ=255)
        tcgtcgtGCTAAAGGCCGCGCTCGTCTGACCGTGTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTAACATTCGTCTTCCAGCGGCCACAACgg                                                                                                                               >  1:1402327/1‑140 (MQ=255)
                   aaGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCGGCCACAACGGGCTGGCAcgcc                                                                                                                    >  2:1278763/1‑140 (MQ=255)
                   aaGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCGGCCACAACGGGCTGGCAcgcc                                                                                                                    >  2:2795168/1‑140 (MQ=255)
                   aaGGCCGCGCTCGTCTGACCGTTTCGAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACAGTCGTCTTCCAGCGGCCACACCGGGCTGGCAcgcc                                                                                                                    >  2:2577257/1‑140 (MQ=255)
                        cgcgCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCGGCCACAACGGGCTGGCACGCCGCaaa                                                                                                               >  2:186857/1‑140 (MQ=255)
                        cgcgCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGATAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTAACATTCGTCTTCCAGCGGCCCCAACGGGCTGGCACGCGGCaaa                                                                                                               >  2:1997866/1‑140 (MQ=255)
                         gcgcTCGTCTGACCGTTTCTAAGGAATAAAGCTAACCCCTGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCGGCCACAACGGGCTGGCACGCCGCAAAt                                                                                                              >  1:961045/1‑140 (MQ=255)
                          cgcTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCGGCCACAACGGGCTGGCACGCCGCAAAtt                                                                                                             >  2:1096884/1‑140 (MQ=255)
                               gTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCGGCCACAACGGGCTGGCACGCCGCAAATTACCAt                                                                                                        >  2:3076515/1‑140 (MQ=255)
                                         ttCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCGGCCACAACGGGCTGGCACGCCGCACATTACCATTCTCGgccgc                                                                                              >  2:3765520/1‑140 (MQ=255)
                                         ttCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCGGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGgccgc                                                                                              >  2:3476945/1‑140 (MQ=255)
                                         ttCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCGGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGgccgc                                                                                              >  2:3208468/1‑140 (MQ=255)
                                             aaGTAATAAAGCTAACCCCTGAGTGGTTAAGCTCGCATTTCCCAGGGAGTGACGCTTGTTAACTCCCAGTCAATTCACATTCGTCGTCCAGCGGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGa                                                                                          <  2:101805/140‑1 (MQ=255)
                                              aGTAATAAAGCTAACCCCTGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCGGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGaa                                                                                         <  2:2632528/140‑1 (MQ=255)
                                              aGTAATAAAGCTAACCCCTGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCGGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGaa                                                                                         <  1:2994273/140‑1 (MQ=255)
                                              aGTAATAAAGCTAACCCCTGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCGGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGaa                                                                                         <  2:2905372/140‑1 (MQ=255)
                                                   taaAGCTAACCCCTGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCGGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGc                                                                                    >  1:2362572/1‑140 (MQ=255)
                                                   taaAGCTAACCCCTGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCGGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGc                                                                                    <  1:637723/140‑1 (MQ=255)
                                                        gtAACCCCGGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCGGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTgggg                                                                               <  2:2964459/139‑1 (MQ=255)
                                                        cTAACCCCTGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCGGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTgggg                                                                               <  1:3497165/140‑1 (MQ=255)
                                                        cTAACCCCTGAGTGGTTAAGCTCGCATTACCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCGGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTgggg                                                                               <  2:3053549/140‑1 (MQ=255)
                                                          aaCCCCTGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCGGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCa                                                                             >  1:676811/1‑140 (MQ=255)
                                                          aaCCCCTGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCGGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCa                                                                             >  1:3561735/1‑140 (MQ=255)
                                                          aaCCCCTGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCGGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCa                                                                             >  1:1664688/1‑140 (MQ=255)
                                                          aaCCCCTGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCGGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCa                                                                             >  1:312934/1‑140 (MQ=255)
                                                           aCCCCTGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCGGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCAt                                                                            >  2:3578427/1‑140 (MQ=255)
                                                             cccTGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCGGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATcc                                                                          <  2:1629129/140‑1 (MQ=255)
                                                                tGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCGGCCACAACGGGCTGGCACGCCGCTCAGTCCCCTACTCGGCCGCCTGACTTCGCTGGGGCCTCCCCg                                                                       >  2:2237738/1‑140 (MQ=255)
                                                                tGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCGGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCg                                                                       >  2:3187757/1‑140 (MQ=255)
                                                                  aGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCTCAGTCAATGCACATTCGTCTTCCAGCGGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTa                                                                     >  2:2332547/1‑140 (MQ=255)
                                                                  aGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCGGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCCTCCCCGTa                                                                     >  2:3321326/1‑140 (MQ=255)
                                                                  aGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCGGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTa                                                                     >  2:1162657/1‑140 (MQ=255)
                                                                  aGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCGGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTa                                                                     >  2:785866/1‑140 (MQ=255)
                                                                  aGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCGGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTa                                                                     >  2:1928684/1‑140 (MQ=255)
                                                                  aGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCGGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTa                                                                     >  2:2589722/1‑140 (MQ=255)
                                                                  aGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCGGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTa                                                                     >  2:1132368/1‑140 (MQ=255)
                                                                   gTGGTTAAGCTCGCATTTCCGAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCGGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTAt                                                                    <  2:518852/140‑1 (MQ=255)
                                                                   gTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCACTTCACATTCGTCTTCCAGCGGCCACAACGGGCTGGCACGCCGCAAATGACCAGTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTAt                                                                    <  2:1510078/140‑1 (MQ=255)
                                                                   gTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCGGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTAt                                                                    <  1:1096884/140‑1 (MQ=255)
                                                                   gTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCGGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTAt                                                                    <  2:312934/140‑1 (MQ=255)
                                                                   gTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCGGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTAt                                                                    <  1:1285661/140‑1 (MQ=255)
                                                                   gTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCGGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTAt                                                                    <  2:3715040/140‑1 (MQ=255)
                                                                   gTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCGGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTAt                                                                    <  2:1664688/140‑1 (MQ=255)
                                                                   gTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCGGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTAt                                                                    <  2:1467405/140‑1 (MQ=255)
                                                                   gTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCGGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTAt                                                                    <  1:3457942/140‑1 (MQ=255)
                                                                   gTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCGGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTAt                                                                    <  1:1032389/140‑1 (MQ=255)
                                                                   gTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCGGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTAt                                                                    <  2:1419761/140‑1 (MQ=255)
                                                                   gTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCGGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTAt                                                                    <  1:446588/140‑1 (MQ=255)
                                                                   gTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCGGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTAt                                                                    <  1:3838577/140‑1 (MQ=255)
                                                                   gTGGTTAAGCTCGCATGTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCGGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTAt                                                                    <  2:2279711/140‑1 (MQ=255)
                                                                       ttAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCGGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGt                                                                >  2:3503481/1‑140 (MQ=255)
                                                                         aaGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCTCATTCGTCTTCCAGCGGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGCATTCGCTGGGGCATCCCCGTATCGGTCt                                                              >  1:1221111/1‑140 (MQ=255)
                                                                              cGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCGGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAg                                                         >  1:3407605/1‑140 (MQ=255)
                                                                                cATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCGGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTc                                                       >  1:2554964/1‑140 (MQ=255)
                                                                                cATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCGGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTc                                                       >  1:2784051/1‑140 (MQ=255)
                                                                                 aTTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCGGCCACACCGGGCTGGCACGCCGCAAATTACCATTCGCGGCCGCCTGTATTCGCTGGGGCATCCCCGTCTCGGTCTTACAGTCg                                                      >  2:2806676/1‑140 (MQ=255)
                                                                                         gggAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCGGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGaaa                                              <  2:2510525/140‑1 (MQ=255)
                                                                                          ggAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCGGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGaaaa                                             >  1:1472024/1‑140 (MQ=255)
                                                                                           gAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCGGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCCTCCCCGTATCGGTCTTACAGTCGCCAAGaaaaa                                            >  2:869214/1‑140 (MQ=255)
                                                                                              ttACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCGGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGt                                         >  2:3441843/1‑140 (MQ=255)
                                                                                                aCGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCGGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTc                                       >  1:2468149/1‑140 (MQ=255)
                                                                                                   cTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCGGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGAc                                    >  1:1914262/1‑140 (MQ=255)
                                                                                                    ttGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCGGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGAcg                                   >  1:3536100/1‑140 (MQ=255)
                                                                                                    ttGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCGGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTATTACAGTCGCCAAGAAAAACGTTCGAcg                                   <  1:85782/140‑1 (MQ=255)
                                                                                                      gTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCGGCCACAACGGGCTGGCACGCCGCCAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGAcgcg                                 >  1:1974821/1‑140 (MQ=255)
                                                                                                      gTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCGGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGAcgcg                                 >  1:3206499/1‑140 (MQ=255)
                                                                                                      gTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCGGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGAcgcg                                 >  1:528997/1‑140 (MQ=255)
                                                                                                      gTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCGGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGAcgcg                                 >  1:3449561/1‑140 (MQ=255)
                                                                                                      gTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCGGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGAcgcg                                 >  2:3670204/1‑140 (MQ=255)
                                                                                                      gTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCGGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGAcgcg                                 >  2:3410306/1‑140 (MQ=255)
                                                                                                      gTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCGGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGAcgcg                                 >  1:3191643/1‑140 (MQ=255)
                                                                                                      gTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCGGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGAcgcg                                 >  1:3097989/1‑140 (MQ=255)
                                                                                                      gTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCGGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGAcgcg                                 >  1:3057399/1‑140 (MQ=255)
                                                                                                      gTTAACTACCAGTCAATTCACATTCGTCATCAAGCGGCCACAACGGGATGGCACGCCGCAAATTACAATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGACTTACAGTCGCCAAGAAAAACCTTCGAcgag                                 >  2:3899074/1‑138 (MQ=255)
                                                                                                         aaCTCCCAGTCAATTCACATTCGTCTTCCAGCGGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGccc                              <  1:1433166/140‑1 (MQ=255)
                                                                                                            tCCCAGTCAATTCACATTCGTCTTCCAGCGGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATg                           >  1:2222088/1‑140 (MQ=255)
                                                                                                              ccAGTCAATTCACATTCGTCTTCCAGCGGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGaa                         <  2:3281670/140‑1 (MQ=255)
                                                                                                                aGTCAATTCACATTCGTCTTCCAGCGGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTAt                                                                    <  1:1793618/95‑1 (MQ=255)
                                                                                                                aGTCAATTCACATTCGTCTTCCAGCGGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACg                       >  1:280932/1‑140 (MQ=255)
                                                                                                                aGTCAATTCACATTCGTCTTCCAGCGGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACg                       >  1:3303049/1‑140 (MQ=255)
                                                                                                                aGTCAATTCACATTCGTCTTCCAGCGGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACg                       >  2:1779983/1‑140 (MQ=255)
                                                                                                                  tCAATTCACATTCGTCTTCCAGCGGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCa                     <  1:1651486/140‑1 (MQ=255)
                                                                                                                      ttCACATTCGTCTTCCAGCGGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCg                 >  2:1696728/1‑140 (MQ=255)
                                                                                                                      ttCACATTCGTCTTCCAGCGGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCg                 >  2:3846894/1‑140 (MQ=255)
                                                                                                                      ttCACATTCGTCTTCCAGCGGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCg                 >  1:3333428/1‑140 (MQ=255)
                                                                                                                           atccGTCTTCCAGCGGCCACACCGGGCTGGCACGCCCCAAAATACCATACACGCCCGCCTGAATTCGCTCCGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAAACGGATTa            <  2:3595076/137‑1 (MQ=255)
                                                                                                                            ttCGTCTTCCAGCGGCCACATCGGGCAGGCACGGCGCCAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCTGTCTTACAGTCGCCCAGAAAAACGTTCGACGCGCCCATGAACGCACTCGGATTaa           >  2:617732/1‑140 (MQ=255)
                                                                                                                                tCTTCCAGCGGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTCTCGGTCTTACAGTCGCCCAGAAAAACGTTCGACGCGCCCATGAACGCAAGCGGATTAAACGt       >  1:2903933/1‑140 (MQ=255)
                                                                                                                                tCTTCCAGCGGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGt       >  2:283802/1‑140 (MQ=255)
                                                                                                                                tCTTCCAGCGGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGt       >  1:1136778/1‑140 (MQ=255)
                                                                                                                                     cagcggCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGAc  >  1:2004848/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                       
CTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGAC  >  CP009273/3877786‑3878058

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A