Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A0 F0 I1 R1 12 93.4 3336394 91.1% 3039454 143.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,768,131 A→T pseudogene (911/945 nt) rhsJ → Rhs‑family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,768,1310AT97.8% 309.1 / NA 92pseudogene (911/945 nt)rhsJRhs‑family protein
Reads supporting (aligned to +/- strand):  ref base A (0/2);  new base T (49/40);  total (50/42)
Fisher's exact test for biased strand distribution p-value = 2.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAGGAACTAATGGTAAGTTAAAAATGGATACAGTGCTAACAGCACCAACGAACCTTAATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCAAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTAC  >  NC_000913/3767984‑3768277
                                                                                                                                                   |                                                                                                                                                  
gAGGAACTAATGGTAAGTTAAAAATGGATACAGTGCTAACAGCACCAACGAACCTTAATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAg                                                                                                                                                  <  2:844205/150‑1 (MQ=255)
gAGGAACTAATGGTAAGTTAAAAATGGATACAGTGCTAACAGCACCAACGAACCTTAATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAg                                                                                                                                                  <  2:890838/150‑1 (MQ=255)
 aGGAACTAATGGTAAGTTAAAAATGGATACAGTGCTAACAGCACCAACGAACCTTAATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAgg                                                                                                                                                 <  1:785912/150‑1 (MQ=255)
 aGGAACTAATGGTAAGTTAAAAATGGATACAGTGCTAACAGCACCAACGAACCTTAATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAgg                                                                                                                                                 <  2:924401/150‑1 (MQ=255)
        aaTGGTAAGTTAAAAATGGATACAGTGCTAACAGCACCAACGAACCTTAATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCa                                                                                                                                          >  4:26719/1‑150 (MQ=255)
          tGGTAAGTTAAAAATGGATACAGTGCTAACAGCACCAACGAACCTTAATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGa                                                                                                                                        >  4:175774/1‑150 (MQ=255)
           ggTAAGTTAAAAATGGATACAGTGCTAACAGCACCAACGAACCTTAATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGaa                                                                                                                                       >  4:162015/1‑150 (MQ=255)
           ggTAAGTTAAAAATGGATACAGTGCTAACAGCACCAACGAACCTTAATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGaa                                                                                                                                       >  1:1106659/1‑150 (MQ=255)
            gTAAGTTAAAAATGGATACAGTGCTAACAGCACCAACGAACCTTAATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGaaa                                                                                                                                      >  2:513964/1‑150 (MQ=255)
             tAAGTTAAAAATGGATACAGTGCTAACAGCACCAACGAACCTTAATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGATCAGTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGaaaa                                                                                                                                     >  1:315436/1‑150 (MQ=255)
                gTTAAAAATGGATACAGTGCTAACAGCACCAACGAACCTTAATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAAcc                                                                                                                                  <  4:388031/150‑1 (MQ=255)
                gTTAAAAATGGATACAGTGCTAACAGCACCAACGAACCTTAATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAAcc                                                                                                                                  <  2:283695/150‑1 (MQ=255)
                 ttAAAAATGGATACAGTGCTAACAGCACCAACGAACCTTAATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAAccc                                                                                                                                 >  2:939534/1‑150 (MQ=255)
                  tAAAAATGGATACAGTGCTAACAGCACCAACGAACCTTAATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCa                                                                                                                                <  2:266945/150‑1 (MQ=255)
                   aaaaaTGGATACAGTGCTAACAGCACCAACGAACCTTAATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAg                                                                                                                               <  2:698044/150‑1 (MQ=255)
                      aaTGGATACAGTGCTAACAGCACCAACGAACCTTAATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGa                                                                                                                            <  1:939534/150‑1 (MQ=255)
                      aaTGGATACAGTGCTAACAGCACCAACGAACCTTAATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGa                                                                                                                            <  1:530703/150‑1 (MQ=255)
                      aaTGGATACAGTGCTAACAGCACCAACGAACCTTAATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGa                                                                                                                            <  1:430246/150‑1 (MQ=255)
                       atagATACAGTGCTAACAGCACCAACGAAACTTAATTCAAAAAAGGTTCTAATGGCTATAATAACAAACGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGat                                                                                                                           <  4:50346/147‑1 (MQ=255)
                            tACAGTGCTAACAGCACCAACGAACCTTAATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAg                                                                                                                      <  1:240175/150‑1 (MQ=255)
                            tACAGTGCTAACAGCACCAACGAACCTTAATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAg                                                                                                                      >  4:180574/1‑150 (MQ=255)
                               aGTGCTAACAGCACCAACGAACCTTAATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATa                                                                                                                   >  3:448265/1‑150 (MQ=255)
                               aGTGCTAACAGCACCAACGAACCTTAATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATa                                                                                                                   >  1:806274/1‑150 (MQ=255)
                                  gCTAACAGCACCAACGAACCTTAATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGt                                                                                                                <  2:771637/150‑1 (MQ=255)
                                   cTAACAGCACCAACGAACCTTAATTCAAATAAGGATCTAATGGCTATCACAACAAAAGACTTTGGTAATTGGATAACATATGTGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCAAGTGATATAAGATAGAtt                                                                                                               <  4:21006/150‑1 (MQ=255)
                                    tAACAGCACCAACGAACCTTAATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTg                                                                                                              <  1:513964/150‑1 (MQ=255)
                                     aaCAGCACCAACGAACCTTAATTCAAATAAGGATCTACTGGCTATAACAACAAAAGACCTCGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGACAGGTCGt                                                                                                             >  2:728700/1‑150 (MQ=255)
                                     aaCAGCACCAACGAACCTTAATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCCCCCCATTTCTAATAGGCCAGGTACGCCACAACAACCCAGTGATATAAGCTAgctcgc                                                                                                             >  2:728784/1‑145 (MQ=255)
                                      aCAGCACCAACGAACCTTAATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTa                                                                                                            >  1:881884/1‑150 (MQ=255)
                                      aCAGCACCAACGAACCTTAATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTa                                                                                                            >  2:737123/1‑150 (MQ=255)
                                      aCAGCACCAACGAACCTTAATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTa                                                                                                            >  4:337367/1‑150 (MQ=255)
                                          cACCAACGAACCTTAATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATg                                                                                                        >  1:999134/1‑150 (MQ=255)
                                              aaCGAACCTTAATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATg                                                                                                    >  2:425044/1‑150 (MQ=255)
                                                 gAACCTTAATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGaaa                                                                                                 <  3:318349/150‑1 (MQ=255)
                                                     cTTAATTCAAATAGGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCAt                                                                                             <  2:11231/150‑1 (MQ=255)
                                                        aaTACAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCt                                                                                          <  1:66170/150‑1 (MQ=255)
                                                         aTTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCtt                                                                                         >  1:112701/1‑150 (MQ=255)
                                                         aTTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCtt                                                                                         >  2:852118/1‑150 (MQ=255)
                                                         aTTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCtt                                                                                         >  2:26901/1‑150 (MQ=255)
                                                          ttCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTg                                                                                        >  1:294098/1‑150 (MQ=255)
                                                          ttCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTg                                                                                        >  3:511261/1‑150 (MQ=255)
                                                           tCAAATAAGGATCTAATGGCTATAAAAACAAAAAACTTTGGAAAGTGGATGAGATAAGGGACATCTGCGCACCCCATTTCTTTTATTCAAAAAACGAAAAAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGt                                                                                       <  4:75778/150‑1 (MQ=255)
                                                             aaaTAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTa                                                                                     >  4:67004/1‑150 (MQ=255)
                                                               aTAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACt                                                                                   <  4:55467/150‑1 (MQ=255)
                                                               aTAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATAAGGGCCATCTGGGCACCCCATTTCTAATATGCAAAAAAAAAAAAAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACt                                                                                   <  4:55458/150‑1 (MQ=255)
                                                                 aaGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCtt                                                                                         >  1:1037537/1‑142 (MQ=255)
                                                                 aaGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCtt                                                                                         <  2:1037537/142‑1 (MQ=255)
                                                                 aaGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTAt                                                                                 >  1:1090465/1‑150 (MQ=255)
                                                                     aTCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCGGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTAttttt                                                                             <  1:356666/150‑1 (MQ=255)
                                                                         aaTGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGaaaaa                                                                                                                                    >  3:174634/1‑91 (MQ=255)
                                                                         aaTGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGaaaaa                                                                                                                                    <  4:174634/91‑1 (MQ=255)
                                                                          aTGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTAtt                                                                        >  3:244241/1‑150 (MQ=255)
                                                                           tGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTAttt                                                                       <  2:66038/150‑1 (MQ=255)
                                                                           tGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAAGAAAAAAAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTAttt                                                                       <  2:315436/150‑1 (MQ=255)
                                                                                  aacaacAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCtttt                                                                >  2:1152665/1‑150 (MQ=255)
                                                                                  aacaacAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCtttt                                                                >  2:952572/1‑150 (MQ=255)
                                                                                    caacaaAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTAt                                                              >  4:147444/1‑150 (MQ=255)
                                                                                     aacaaAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATACGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTAtt                                                             >  2:104750/1‑150 (MQ=255)
                                                                                      acaaAAGACCTTTGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTAttt                                                            >  2:371817/1‑150 (MQ=255)
                                                                                       caaAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTAt                                                                                                     >  4:270264/1‑108 (MQ=255)
                                                                                       caaAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTAt                                                                                                     <  3:270264/108‑1 (MQ=255)
                                                                                        aaaaGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTAttt                                                                       >  1:627182/1‑137 (MQ=255)
                                                                                        aaaaGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTAttt                                                                       <  2:627182/137‑1 (MQ=255)
                                                                                        aaaaGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGt                                                          <  3:175774/150‑1 (MQ=255)
                                                                                        aaaaGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGt                                                          >  2:837501/1‑150 (MQ=255)
                                                                                           aGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCaa                                                       >  2:781964/1‑150 (MQ=255)
                                                                                           aGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCaa                                                       >  2:997983/1‑150 (MQ=255)
                                                                                            gACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTTTCTTTTATTTGTCAAt                                                      >  4:407361/1‑150 (MQ=255)
                                                                                               cTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGc                                                   >  2:2651/1‑150 (MQ=255)
                                                                                                ttGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTgaa                                                  >  4:305384/1‑148 (MQ=255)
                                                                                                ttGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCa                                                  >  3:190114/1‑150 (MQ=255)
                                                                                                ttGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCa                                                  >  1:926779/1‑150 (MQ=255)
                                                                                                  ggTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATg                                                <  3:469132/150‑1 (MQ=255)
                                                                                                      aTTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGt                                                                                       <  2:800821/107‑1 (MQ=255)
                                                                                                      aTTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGt                                                                                       >  1:800821/1‑107 (MQ=255)
                                                                                                                   tGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATa                               <  3:67004/150‑1 (MQ=255)
                                                                                                                      gCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATaaaa                            <  2:1123105/150‑1 (MQ=255)
                                                                                                                      gCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCGTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATaaaa                            <  2:527776/150‑1 (MQ=255)
                                                                                                                        cATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGAGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATaaaaca                          >  1:443089/1‑150 (MQ=255)
                                                                                                                          tCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAAc                                                                                                                                   >  4:449931/1‑43 (MQ=255)
                                                                                                                          tCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAAc                                                                                                                                   <  3:449931/43‑1 (MQ=255)
                                                                                                                           cTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATaaaacaaaa                       <  4:202362/150‑1 (MQ=255)
                                                                                                                           cTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATaaaacaaaa                       <  2:999134/150‑1 (MQ=255)
                                                                                                                            tGGGCACCCCATTTCTAATATGCTCGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATaaaacaaaac                      >  4:118981/1‑150 (MQ=255)
                                                                                                                            tGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAAATTCTTGGTGCTGATGTGAAAATaaaacaaaac                      >  1:833925/1‑150 (MQ=255)
                                                                                                                                cACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGaaa                  <  2:806274/150‑1 (MQ=255)
                                                                                                                                         tCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCGTGCTTGTTACTGTTTTAGTAGTGCTCTTTTATTTTGCAATTGCATTGTGCTGATGGGAAAATAAAACAAAACGAAATGATGATgg         >  1:581767/1‑150 (MQ=255)
                                                                                                                                          cTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATggg        <  1:93399/150‑1 (MQ=255)
                                                                                                                                           tAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTAttt                                                            >  2:202362/1‑97 (MQ=255)
                                                                                                                                           tAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTAttt                                                            <  1:202362/97‑1 (MQ=255)
                                                                                                                                               atGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTa   <  3:26719/150‑1 (MQ=255)
                                                                                                                                                tGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTAc  >  3:291418/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GAGGAACTAATGGTAAGTTAAAAATGGATACAGTGCTAACAGCACCAACGAACCTTAATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCAAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTAC  >  NC_000913/3767984‑3768277

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGCGAGGAACTAATGGTAAGTTAAAAATGGATACAGTGCTAACAGCACCAACGAACCTTAATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCAAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTAC  >  NC_000913/3767981‑3768277
                                                                                                                                                      |                                                                                                                                                  
GGCGAGGAACTAATGGTAAGTTAAAAATGGATACAGTGCTAACAGCACCAACGAACCTTAATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCT                                                                                                                                                    >  K00180:692:HY2HLBBXX:6:2118:17401:46627/1‑151 (MQ=60)
    AGGAACTAATGGTAAGTTAAAAATGGATACAGTGCTAACAGCACCAACGAACCTTAATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGT                                                                                                                                                <  K00180:692:HY2HLBBXX:6:2120:20101:13816/151‑1 (MQ=60)
             TGGTAAGTTAAAAATGGATACAGTGCTAACAGCACCAACGAACCTTAATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAA                                                                                                                                       >  K00180:692:HY2HLBBXX:6:2223:5193:42495/1‑151 (MQ=60)
               GTAAGTTAAAAATGGATACAGTGCTAACAGCACCAACGAACCTTAATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGATCAGTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAA                                                                                                                                     >  K00180:692:HY2HLBBXX:6:1202:9475:46012/1‑151 (MQ=60)
                  actgttctctaactttgtgctaaataatctttttttttttcaagcagaagacggcatacgagattgtggcttgtgactggagttcagacgtgtgctcttccgatctATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACC                                                                                                                                  <  K00180:692:HY2HLBBXX:8:2214:32096:46627/45‑1 (MQ=60)
                         AATGGATACAGTGCTAACAGCACCAACGAACCTTAATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGAT                                                                                                                           <  K00180:692:HY2HLBBXX:6:1213:10490:43163/151‑1 (MQ=60)
                         AATGGATACAGTGCTAACAGCACCAACGAACCTTAATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGAT                                                                                                                           <  K00180:692:HY2HLBBXX:6:1223:19380:15803/151‑1 (MQ=60)
                         AATGGATACAGTGCTAACAGCACCAACGAACCTTAATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGAT                                                                                                                           <  K00180:692:HY2HLBBXX:6:2207:24586:41528/151‑1 (MQ=60)
                               TACAGTGCTAACAGCACCAACGAACCTTAATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGA                                                                                                                     <  K00180:692:HY2HLBBXX:6:1123:24931:21764/151‑1 (MQ=60)
                                 CAGTGCTAACAGCACCAACGAACCTTAATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATA                                                                                                                   >  K00180:692:HY2HLBBXX:6:2122:27803:11899/1‑151 (MQ=60)
                                 CAGTGCTAACAGCACCAACGAACCTTAATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATA                                                                                                                   >  K00180:692:HY2HLBBXX:8:2214:11363:30046/1‑151 (MQ=60)
                                       TAACAGCACCAACGAACCTTAATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGT                                                                                                             <  K00180:692:HY2HLBBXX:6:1221:31294:30714/151‑1 (MQ=60)
                                        AACAGCACCAACGAACCTTAATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTA                                                                                                            >  K00180:692:HY2HLBBXX:6:2202:15605:3776/1‑151 (MQ=60)
                                            GCACCAACGAACCTTAATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATG                                                                                                        >  K00180:692:HY2HLBBXX:6:2213:7040:28991/1‑151 (MQ=60)
                                                gtggcttgtgactggagttcagacgtgtgctcttccgatctACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATG                                                                                                    <  K00180:692:HY2HLBBXX:8:2104:21440:9526/110‑1 (MQ=60)
                                                    GAACCTTAATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAG                                                                                                <  K00180:692:HY2HLBBXX:8:2114:16650:19513/151‑1 (MQ=60)
                                                           AATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTT                                                                                         >  K00180:692:HY2HLBBXX:6:1111:20425:34565/1‑151 (MQ=60)
                                                           AATACAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTT                                                                                         <  K00180:692:HY2HLBBXX:6:1107:3955:15522/151‑1 (MQ=60)
                                                            ATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTG                                                                                        >  K00180:692:HY2HLBBXX:8:2228:18203:17139/1‑151 (MQ=60)
                                                            ATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTG                                                                                        >  K00180:692:HY2HLBBXX:6:1128:25733:42917/1‑151 (MQ=60)
                                                                   TAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGagatcgg                                                                                 >  K00180:692:HY2HLBBXX:6:2217:27306:13675/1‑144 (MQ=60)
                                                                   TAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTAT                                                                                 >  K00180:692:HY2HLBBXX:6:2222:28513:12884/1‑151 (MQ=60)
                                                                        ATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCGGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTT                                                                            <  K00180:692:HY2HLBBXX:6:1206:21805:45467/151‑1 (MQ=60)
                                                                           TAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACagatcggaagagcacacgtctgaactccagtcacaagccacaatctcgtatgccgtct                                                                         >  K00180:692:HY2HLBBXX:8:1211:32177:20217/1‑93 (MQ=60)
                                                                            AATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATT                                                                        >  K00180:692:HY2HLBBXX:8:1226:5964:17456/1‑151 (MQ=60)
                                                                                         catacgagattgtggcttgtgactggagttcagacgtgtgctcttccgatcTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTG                                                           <  K00180:692:HY2HLBBXX:6:1119:32522:42935/100‑1 (MQ=60)
                                                                                          CAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGATTGTTACTATTTTTTTATTTCagatcggaagag                                                          >  K00180:692:HY2HLBBXX:6:2105:21359:4602/1‑139 (MQ=60)
                                                                                           AAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTC                                                         <  K00180:692:HY2HLBBXX:8:1211:28392:34196/151‑1 (MQ=60)
                                                                                                  CTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCA                                                  >  K00180:692:HY2HLBBXX:6:2206:6431:30327/1‑151 (MQ=60)
                                                                                                  CTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCA                                                  >  K00180:692:HY2HLBBXX:8:1214:14357:37660/1‑151 (MQ=60)
                                                                                                  CTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAG                                                                                                                               >  K00180:692:HY2HLBBXX:6:2116:18791:37748/1‑74 (MQ=60)
                                                                                                     GGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGG                                               <  K00180:692:HY2HLBBXX:8:2219:7415:1472/151‑1 (MQ=60)
                                                                                                        AATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTAgatcggaagagcacacgtctgaactccagtcacaagccaca                                            >  K00180:692:HY2HLBBXX:6:2121:5051:33000/1‑110 (MQ=60)
                                                                                                                      TGGGCCATCTAGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAA                              <  K00180:692:HY2HLBBXX:8:1115:2128:29202/151‑1 (MQ=60)
                                                                                                                          CCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGAGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACA                          >  K00180:692:HY2HLBBXX:6:1215:6563:3301/1‑151 (MQ=60)
                                                                                                                              CTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAAATTCTTGGTGCTGATGTGAAAATAAAACAAAAC                      >  K00180:692:HY2HLBBXX:6:2124:25763:47436/1‑151 (MQ=60)
                                                                                                                                           TTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCGTGCTTGTTACTGTTTTAGTAGTGCTCTTTTATTTTGCAATTGCATTGTGCTGATGGGAAAATAAAACAAAACGAAATGATGATGG         >  K00180:692:HY2HLBBXX:6:1228:13971:22045/1‑151 (MQ=60)
                                                                                                                                             CTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGG       <  K00180:692:HY2HLBBXX:6:1109:15635:39277/151‑1 (MQ=60)
                                                                                                                                                  ATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTAC  >  K00180:692:HY2HLBBXX:8:2108:12652:32560/1‑151 (MQ=60)
                                                                                                                                                  ATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTAC  <  K00180:692:HY2HLBBXX:8:1106:5122:46838/151‑1 (MQ=60)
                                                                                                                                                      |                                                                                                                                                  
GGCGAGGAACTAATGGTAAGTTAAAAATGGATACAGTGCTAACAGCACCAACGAACCTTAATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCAAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTCTAC  >  NC_000913/3767981‑3768277

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑