Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A0 F0 I1 R1 245 579.6 28867798 97.7% 28203838 96.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,537,405 +GC intergenic (+61/‑394) b3407 → / → feoA hypothetical protein/ferrous iron transport protein A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,537,4041.C98.8% 1536.0 / 7.4 408intergenic (+60/‑395)b3407/feoAhypothetical protein/ferrous iron transport protein A
Reads supporting (aligned to +/- strand):  ref base . (1/4);  new base C (209/194);  total (210/198)
Fisher's exact test for biased strand distribution p-value = 2.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01
*NC_0009133,537,4042.G98.8% 1509.3 / 7.2 407intergenic (+60/‑395)b3407/feoAhypothetical protein/ferrous iron transport protein A
Reads supporting (aligned to +/- strand):  ref base . (1/4);  new base G (208/194);  total (209/198)
Fisher's exact test for biased strand distribution p-value = 2.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.24e-01

Only 100 of 457 total aligned reads displayed.

GATGCGCTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CA‑‑C‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATA  >  NC_000913/3537309‑3537490
                                                                                                  ||                                                                                        
gatgCGCTGGCGGCGGCAATGGGCAAAAAACGTTAAGCGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGACAGATAAGGCGTT‑‑Ca                                                                                            <  1:12882995/96‑1 (MQ=255)
 atgCGCTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCAGGAAATCGTAGGGCAGATAAGGCGTT‑‑CA‑‑c                                                                                         <  2:13914130/96‑1 (MQ=255)
 atgCGCTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CA‑‑c                                                                                         <  1:6431758/96‑1 (MQ=255)
 atgCGCTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CA‑‑c                                                                                         <  1:11923854/96‑1 (MQ=255)
  tgCGCTCGCCGCGCCAATGGTCAAAAAACGTGAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑Catg                                                                                          <  1:10645225/96‑3 (MQ=255)
    cgcTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CA‑‑cgcc                                                                                      <  2:9618731/96‑4 (MQ=255)
     gcTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CA‑‑cgccg                                                                                     >  2:801544/1‑92 (MQ=255)
           ggcggcAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGC‑‑CGCATCTg                                                                               >  2:8641709/1‑96 (MQ=38)
           ggcggcAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGC‑‑CGCATCTg                                                                               >  1:9190077/1‑96 (MQ=38)
           ggcggcAATGGGAAAAACACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCCCGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGC‑‑CGCATCTg                                                                               <  2:7181253/96‑1 (MQ=37)
             tggcAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGC‑‑CGCATCTGGc                                                                             <  1:13485033/95‑1 (MQ=38)
             cggcAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGC‑‑CGCATCTGGc                                                                             >  1:12423837/1‑96 (MQ=38)
             cggcAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGC‑‑CGCATCTGGc                                                                             <  2:8923398/96‑1 (MQ=38)
             acgcAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGC‑‑CGCATCTGGc                                                                             >  1:11776763/3‑96 (MQ=37)
               gcAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGC‑‑CGCATCTGGCaa                                                                           <  1:10456006/96‑1 (MQ=38)
                cAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGC‑‑CGCATCTGGCaac                                                                          >  1:7462566/1‑96 (MQ=38)
                cAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGC‑‑CGCATCGGGCaac                                                                          >  2:4076119/1‑96 (MQ=37)
                cAATGGGCAAAAAACGTTAAACCCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGC‑‑CGCATCTGGCaac                                                                          <  2:1723597/96‑1 (MQ=37)
                 aaggggCAAAAATCTGTAAACCCCGGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGTCAGATAAGCCGTT‑‑CACGC‑‑CGCATCTGGCaacg                                                                         <  1:9763808/93‑1 (MQ=21)
                 aaTGGGCAAAAAACGTTAAACGCCCGTACTGGCCTATGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGC‑‑CGCATCTGGCaacg                                                                         <  1:12726204/96‑1 (MQ=37)
                 aaTGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGC‑‑CGCATCTGGCaacg                                                                         >  1:7167482/1‑96 (MQ=38)
                 aaTGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGC‑‑CGCATCTGGCaacg                                                                         >  2:9885685/1‑96 (MQ=38)
                 aaTGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGC‑‑CGCATCTGGCaacg                                                                         <  2:11757040/96‑1 (MQ=38)
                  aTGGGCGAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGC‑‑CGCATCTTGCaacga                                                                        >  2:11238057/1‑96 (MQ=37)
                   tGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGC‑‑CGCATCTGGCaacgaa                                                                       >  1:10027496/1‑96 (MQ=38)
                    gggCAAAAAACGTTAAACGCCGGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGC‑‑CGCATCTGGCaacgaac                                                                      <  2:1281604/96‑1 (MQ=37)
                    gggCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGGAGGGCAGATAAGGCGTT‑‑CACGC‑‑CGCATCTGGCaacgaac                                                                      >  1:3648793/1‑96 (MQ=38)
                      gCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCCCGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGC‑‑CGCATCTGGCAACGAACGc                                                                    <  1:11646992/96‑1 (MQ=37)
                      gCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGC‑‑CGCATCTGGCAACGAACGc                                                                    >  1:623662/1‑96 (MQ=38)
                       cAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGC‑‑CGCATCTGGCAACGAACGcc                                                                   <  2:6164017/96‑1 (MQ=38)
                       cAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGC‑‑CGCATCTGGCAACGAACGcc                                                                   <  1:11397932/96‑1 (MQ=38)
                       cAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGC‑‑CGCATCGGGCAACGAACGcc                                                                   >  1:4673339/1‑96 (MQ=37)
                        aaaaaaCGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGC‑‑CGCATCTGGCAACGAACGCCt                                                                  >  1:8413563/1‑96 (MQ=38)
                        aaaaaaCGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGC‑‑CGCATCTGGCAACGAACGCCt                                                                  <  1:6843436/96‑1 (MQ=38)
                         aaaaaCGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCTCGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGC‑‑CGCATCTGGCAACGAACGCCtt                                                                 <  2:8269603/96‑1 (MQ=37)
                         aaaaaCGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGC‑‑CGCATCTGGCAACGAACGCCtt                                                                 >  1:13617744/1‑96 (MQ=38)
                           aaaCGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGC‑‑CGCATCTGGCAACGAACGCCTTGc                                                               >  2:4351842/1‑96 (MQ=38)
                           aaaCGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGC‑‑CGCATCTGGCAACGAACGCCTTGc                                                               <  2:4925089/96‑1 (MQ=38)
                             aCGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGC‑‑CGCATCTGGCAACGAACGCCTTGCCt                                                             <  1:8728217/96‑1 (MQ=38)
                             aCGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGC‑‑CGCATCTGGCAACGAACGCCTTGCCt                                                             >  1:1740499/1‑96 (MQ=38)
                                ttAACCGCCCGTACTGGCTTACGGTTCGAATTTGCACGAAATCGTAGGGAAGATAAGGCGTT‑‑CACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGAc                                                          <  2:7019668/96‑1 (MQ=37)
                                      gCCCGTACGGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATaaaa                                                    <  1:11081108/96‑1 (MQ=37)
                                       cccGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAg                                                   >  2:1200915/1‑96 (MQ=38)
                                       cccGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAg                                                   <  2:4209614/96‑1 (MQ=38)
                                       cccGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAg                                                   >  1:3489469/1‑96 (MQ=38)
                                        ccGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGt                                                  <  2:3216294/96‑1 (MQ=38)
                                         cGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTg                                                 >  1:2762473/1‑96 (MQ=38)
                                         cGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTg                                                 <  1:14414402/96‑1 (MQ=38)
                                          gTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGC‑‑CGCATCTGGGAACGAACGCCTTGCCTGACATAAAAGTGc                                                >  1:6656850/1‑96 (MQ=37)
                                          gTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGc                                                >  1:9633707/1‑96 (MQ=38)
                                          gTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGc                                                >  2:1992786/1‑96 (MQ=38)
                                           tACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGcc                                               <  1:3194244/96‑1 (MQ=38)
                                           tACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGcc                                               >  2:123400/1‑96 (MQ=38)
                                             cTGGCCTACGGTTGGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCgg                                             <  1:801544/96‑1 (MQ=37)
                                             cTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCgg                                             <  2:2583222/96‑1 (MQ=38)
                                             cTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCgg                                             >  1:7066150/1‑96 (MQ=38)
                                              tGGCCTACGGTTCGAATTTGCACGAAATCGTAGGACTGATAGGGCGTT‑‑CACGC‑‑CGTGTCTGGCACCGACCGCCTTGCCTGACATAAAAGTGCCGga                                            <  1:14016314/96‑1 (MQ=255)
                                               ggacTACGGTTCGAATATGCACGAAAACGTAGGGCAGATAAGGCGTC‑‑CACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGgag                                           <  2:9288209/93‑1 (MQ=37)
                                                 ccTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGaa                                         >  1:1307519/1‑96 (MQ=38)
                                                 ccTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGaa                                         >  2:5563089/1‑96 (MQ=38)
                                                 ccTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGaa                                         >  1:3110714/1‑96 (MQ=38)
                                                  cTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAat                                        <  2:5143369/96‑1 (MQ=38)
                                                  cTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAat                                        <  1:252/96‑1 (MQ=38)
                                                  cTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAat                                        <  2:13575552/96‑1 (MQ=38)
                                                    aCGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAatat                                      >  1:8249849/1‑96 (MQ=38)
                                                    aCGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAatat                                      >  1:10221503/1‑96 (MQ=38)
                                                    aCGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAatat                                      >  2:7657836/1‑96 (MQ=38)
                                                     cGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATAtc                                     >  2:4995154/1‑96 (MQ=38)
                                                      ggTTCGATTTGCCACGAATTCGGGGGGCAGATAGGGCGTT‑‑CACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATAtct                                    <  2:2517701/96‑1 (MQ=25)
                                                      ggTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATAtct                                    >  2:7512605/1‑96 (MQ=38)
                                                      ggTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATAtct                                    <  2:5803315/96‑1 (MQ=38)
                                                      ggTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATAtct                                    >  1:14182972/1‑96 (MQ=38)
                                                        ttCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTcc                                  <  1:5843358/96‑1 (MQ=38)
                                                        tacGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTcc                                  >  1:9379476/3‑96 (MQ=37)
                                                          cGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCgg                                >  1:1622614/1‑96 (MQ=38)
                                                           gAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGc                               >  2:14287117/1‑96 (MQ=38)
                                                           gAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGc                               >  1:2260018/1‑96 (MQ=38)
                                                            aaTTTCCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCa                              <  2:8367098/96‑1 (MQ=37)
                                                             aTTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCAt                             >  1:10859975/1‑96 (MQ=38)
                                                              tttGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCAtt                            <  2:10706970/96‑1 (MQ=38)
                                                                   aCGAAATAGTGGGGCAGATAAGGCTTTAACG‑‑C‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTAt                       <  1:5168090/96‑1 (MQ=255)
                                                                    cGAAATCGTAGGGCAGATAAGGCGGT‑‑CACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCGGGAGAATATCTCCGGCATTTTTAtt                      >  1:2103956/1‑96 (MQ=37)
                                                                     gAAATCGTAGGGCAGATAAGGCGTT‑‑CACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGCGAATATCTCCGGCATTTTTATTc                     >  2:6776146/1‑96 (MQ=37)
                                                                     gAAATCGTAGGGCAGATAAGGCGTT‑‑CACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTc                     >  2:10272824/1‑96 (MQ=38)
                                                                         tCGTAGGTCAGATAAGGCGTT‑‑CACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCcaca                 <  2:11123443/96‑1 (MQ=37)
                                                                         tCGTAGGGCAGATAAGGCGTT‑‑CACGC‑‑CGCATCTGGCAACGACCGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCcaca                 <  2:3700231/96‑1 (MQ=37)
                                                                         tCGTAGGGCAGATAAGGCGTC‑‑CACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCcaca                 <  1:121263/96‑1 (MQ=38)
                                                                         tCGTAGGCTAGATAAGGCGTC‑‑CACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCcaca                 <  1:4899630/96‑1 (MQ=37)
                                                                          cGTAGGGCAGATAAGGCGTT‑‑CACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAg                <  2:10156708/96‑1 (MQ=38)
                                                                           gTAGGGCAGATAAGGCGTT‑‑CACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGc               <  2:13414578/96‑1 (MQ=38)
                                                                           gTAGGGCAGATAAGGCGTT‑‑CA‑‑CGACGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGc               <  1:10120247/96‑1 (MQ=39)
                                                                            tAGGGCAGATAAGGCGTT‑‑CACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGcc              <  2:2324196/96‑1 (MQ=38)
                                                                             aGGGCAGATAAGGCGTT‑‑CACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCa             >  2:13662829/1‑96 (MQ=38)
                                                                             aGGGCAGATAAGGCGTT‑‑CACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCa             <  1:4255037/96‑1 (MQ=38)
                                                                               ggCAGATAAGGCGTT‑‑CACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCaaa           <  2:6453585/96‑1 (MQ=38)
                                                                                gCAGATAAGGCGTT‑‑CACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAAc          >  1:5433771/1‑96 (MQ=39)
                                                                                   gATAAGGCGTTCCCG‑‑C‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCa       <  2:2831817/96‑1 (MQ=255)
                                                                                    aTAAGGCGTT‑‑CACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCat      <  1:4351842/96‑1 (MQ=39)
                                                                                     tacggCGTT‑‑CACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCata     <  1:4151525/93‑1 (MQ=39)
                                                                                       aGGCGTT‑‑CACTC‑‑CGCATCTGGCAACGAACTCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCataat   <  1:388168/96‑1 (MQ=255)
                                                                                                  ||                                                                                        
GATGCGCTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTT‑‑CA‑‑C‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATA  >  NC_000913/3537309‑3537490

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 301 total aligned reads displayed.
GATGGATGCGCTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCA‑‑C‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATT  >  NC_000913/3537305‑3537505
                                                                                                      |                                                                                                      
NNNGGATGCGCTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCA‑‑C                                                                                                        <  SRR4302518.9774444/101‑1 (MQ=60)
GATGGATGCGCTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCA‑‑C                                                                                                        <  SRR4302518.6760566/101‑1 (MQ=60)
 NNNGATGCGCTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCA‑‑C‑‑G                                                                                                     <  SRR4302518.14895224/101‑1 (MQ=60)
 ATGGATGCGCTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCA‑‑C‑‑G                                                                                                     <  SRR4302518.332973/101‑1 (MQ=60)
 ATGGATGCGCTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCA‑‑C‑‑G                                                                                                     >  SRR4302518.55785/1‑101 (MQ=60)
 ATGGATGCGCTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCA‑‑C‑‑G                                                                                                     >  SRR4302518.5409016/1‑101 (MQ=60)
   GGATGCGCTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCA‑‑Cgcc                                                                                                     <  SRR4302518.1388578/101‑4 (MQ=60)
   GGATGCGCTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCA‑‑C‑‑NNN                                                                                                   >  SRR4302518.8902838/1‑101 (MQ=60)
    NNNGCGCTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCA‑‑Cgcgg                                                                                                    <  SRR4302518.11088892/101‑5 (MQ=60)
     ATGCGCTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCA‑‑Cgcnnn                                                                                                   >  SRR4302518.8722342/1‑96 (MQ=60)
      nnngcgcGCGGCGTCAACGGGCAAAAAACGTCAAACGCCCGTACTGGCCTACGGTTGGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCA                                                                                                  <  SRR4302518.13941390/94‑1 (MQ=60)
        CGCTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATC                                                                                                >  SRR4302518.7263821/1‑101 (MQ=60)
        CGCTCGCGGCGTCACTGGGCAAAAAACGTTAAACGCCCGCCCTGGCCTACGGTCCGAATTTGCACGAAATCGTAGGGAAGATAAGGCGTTCACGC‑‑CGCATC                                                                                                <  SRR4302518.96769/101‑1 (MQ=60)
         GTTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCA‑‑Cgccgcannn                                                                                               >  SRR4302518.11209862/1‑92 (MQ=60)
         GCTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGATATCGTAGGGCAGATAAGGCGTTCA‑‑Cgccgcannn                                                                                               >  SRR4302518.7467743/1‑92 (MQ=60)
         GCTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCA‑‑Cgccgcatcg                                                                                               >  SRR4302518.2547375/1‑92 (MQ=60)
          ctgtgggccGCAATGGGCAAAAGACCTTGAACGCCCGTACTGGCCTACTGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTG                                                                                              <  SRR4302518.4443789/92‑1 (MQ=60)
          NNNGCGGCGGCAATGGGCAAAAAACGTTAACCGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCATAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTG                                                                                              <  SRR4302518.12931341/101‑1 (MQ=60)
          CTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTG                                                                                              >  SRR4302518.4131753/1‑101 (MQ=60)
          CTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTG                                                                                              >  SRR4302518.3361729/1‑101 (MQ=60)
          CTGGCGGCGGCAATGGGAAAAAACCGTTAAACGCCCGTACTGGCCTACGGTGCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTG                                                                                              <  SRR4302518.196718/101‑1 (MQ=60)
           TGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCA‑‑Cgccgcatcnnn                                                                                             >  SRR4302518.7813220/1‑90 (MQ=60)
           TGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTGGCACGAAATCGTAGGGCAGATAAGGCGTTCA‑‑Cgccgcatcnnn                                                                                             >  SRR4302518.13715667/1‑90 (MQ=60)
           GGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATGGGAGGGCAGATAAGGCGTTCA‑‑Cgccgcatcnnn                                                                                             >  SRR4302518.11921913/1‑90 (MQ=60)
             GCGGCGGCAAGGGGCAAAAAACGTTAAACGCCCGTACGGGCCTACGGGCCGAATTTGCACGAAATCGTAAGGCAGATAATGCGTTCA‑‑Cgccgcatccgnnn                                                                                           >  SRR4302518.10019824/1‑88 (MQ=60)
              NNNCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAA                                                                                          <  SRR4302518.10945817/101‑1 (MQ=60)
              CGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAA                                                                                          >  SRR4302518.5158870/1‑101 (MQ=60)
               GGCGGCAACGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAAC                                                                                         <  SRR4302518.5673066/101‑1 (MQ=60)
                nnngcaaggggcaaaaatctgtaaaccccgGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGTCAGATAAGCCGTTCACGC‑‑CGCATCTGGCAACG                                                                                        <  SRR4302518.10232270/71‑1 (MQ=57)
                NNNGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTATGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACG                                                                                        <  SRR4302518.13320581/101‑1 (MQ=60)
                 acGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAANNN                                                                                       >  SRR4302518.12334256/3‑101 (MQ=60)
                 CGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAANNN                                                                                       >  SRR4302518.13009045/1‑101 (MQ=60)
                 CGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑AGCATCTGGCAACGA                                                                                       >  SRR4302518.2031598/1‑101 (MQ=60)
                  GGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAA                                                                                      <  SRR4302518.1079161/101‑1 (MQ=60)
                   NNNATGGGCAAAAAACTTTAACCGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGAAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAAC                                                                                     <  SRR4302518.12614466/101‑1 (MQ=60)
                   GCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAAC                                                                                     <  SRR4302518.3950896/101‑1 (MQ=60)
                    NNNTGGACAAAAAACGTTAACCCCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACG                                                                                    <  SRR4302518.13567496/101‑1 (MQ=60)
                    CAATTGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAAGTTGCACGAAATCGTAGGTCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACG                                                                                    <  SRR4302518.7027202/101‑1 (MQ=60)
                    CAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGANNN                                                                                    >  SRR4302518.7847284/1‑101 (MQ=60)
                    CAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGANNN                                                                                    >  SRR4302518.13232634/1‑101 (MQ=60)
                    CAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACG                                                                                    <  SRR4302518.4666255/101‑1 (MQ=60)
                     NNNGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCCCGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGC                                                                                   <  SRR4302518.12195232/101‑1 (MQ=60)
                     AATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAANNN                                                                                   >  SRR4302518.7543977/1‑101 (MQ=60)
                     AATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGC                                                                                   >  SRR4302518.2397262/1‑101 (MQ=60)
                      NNNGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCC                                                                                  <  SRR4302518.11931853/101‑1 (MQ=60)
                      ATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACNNN                                                                                  >  SRR4302518.12559383/1‑101 (MQ=60)
                      ACGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGGAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACNNN                                                                                  >  SRR4302518.12378465/1‑101 (MQ=60)
                       agcGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCT                                                                                 <  SRR4302518.7205566/98‑1 (MQ=60)
                       TGGGCAAAAAACGTTAAAGGCCCGTACTGGCCTACGGTTAGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGNNN                                                                                 >  SRR4302518.10651590/1‑101 (MQ=60)
                       TGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGNNN                                                                                 >  SRR4302518.10503670/1‑101 (MQ=60)
                        NNNCAAAAAACGTTACGCGACCGTACTGGCCTACGATTAGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTT                                                                                <  SRR4302518.10425339/101‑1 (MQ=58)
                        GGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCACCGAACGCCTT                                                                                >  SRR4302518.5710742/1‑101 (MQ=60)
                        GGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTT                                                                                >  SRR4302518.5275727/1‑101 (MQ=60)
                        GGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGGAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTT                                                                                >  SRR4302518.3819873/1‑101 (MQ=60)
                         GGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCNNN                                                                               >  SRR4302518.14149642/1‑101 (MQ=60)
                          GCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGC                                                                              >  SRR4302518.4464552/1‑101 (MQ=60)
                          GCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGC                                                                              >  SRR4302518.647854/1‑101 (MQ=60)
                           CAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTNNN                                                                             >  SRR4302518.11102613/1‑101 (MQ=60)
                           CAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCGGGCAACGAACGCCTTGCC                                                                             >  SRR4302518.4919201/1‑101 (MQ=60)
                            nnngacCGTTACACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCTT                                                                            <  SRR4302518.11116353/95‑1 (MQ=60)
                            AAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGNNN                                                                            >  SRR4302518.8825923/1‑101 (MQ=60)
                            AAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGNNN                                                                            >  SRR4302518.15102056/1‑101 (MQ=60)
                            AAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCT                                                                            >  SRR4302518.3070469/1‑101 (MQ=60)
                            AAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCT                                                                            >  SRR4302518.3738499/1‑101 (MQ=60)
                             AAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCNNN                                                                           >  SRR4302518.14242171/1‑101 (MQ=60)
                             AAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTG                                                                           >  SRR4302518.3644971/1‑101 (MQ=60)
                             AAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTG                                                                           >  SRR4302518.5231800/1‑101 (MQ=60)
                              NNNACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGA                                                                          <  SRR4302518.12479603/101‑1 (MQ=60)
                               AAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGAC                                                                         <  SRR4302518.4054891/101‑1 (MQ=60)
                                nnnattaatctCCCGTACTTGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACA                                                                        <  SRR4302518.12482610/90‑1 (MQ=60)
                                ACCGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGNNN                                                                        >  SRR4302518.8170639/1‑101 (MQ=60)
                                AACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACA                                                                        >  SRR4302518.7283809/1‑101 (MQ=60)
                                 ACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACAT                                                                       >  SRR4302518.1818142/1‑101 (MQ=60)
                                  NNNTAACCGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATA                                                                      <  SRR4302518.13106396/101‑1 (MQ=60)
                                    TTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAA                                                                    >  SRR4302518.1412452/1‑101 (MQ=60)
                                     TAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATANNN                                                                   >  SRR4302518.10666137/1‑101 (MQ=60)
                                     TAAACGCCAGTACTGGCCTGCGGTTCGAATTTGCACGAATTCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAA                                                                   <  SRR4302518.6148322/101‑1 (MQ=60)
                                     NNNACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAA                                                                   <  SRR4302518.9435694/101‑1 (MQ=60)
                                     NNNACGCCCGTACGGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAA                                                                   <  SRR4302518.11599427/101‑1 (MQ=60)
                                      nnntgcctGTACTGGCATACGGTTCGAATTTGCTCAAAATCGTTGGGCAGTTAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTTCGTGACATAAAAG                                                                  <  SRR4302518.13791673/93‑1 (MQ=40)
                                      AAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGTACGCCTTGCCTGACATAANNN                                                                  >  SRR4302518.13383049/1‑101 (MQ=60)
                                       NNNGCCCGTCCTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGT                                                                 <  SRR4302518.13492095/101‑1 (MQ=60)
                                       NNNGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGT                                                                 <  SRR4302518.7492448/101‑1 (MQ=60)
                                       NNNGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGT                                                                 <  SRR4302518.12059290/101‑1 (MQ=60)
                                       AACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGG                                                                 >  SRR4302518.6596596/1‑101 (MQ=60)
                                        NNNCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTG                                                                <  SRR4302518.15083891/101‑1 (MQ=60)
                                        NNNCCCGGACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTT                                                                <  SRR4302518.13706844/101‑1 (MQ=60)
                                         nnnccgtctgGGCCTAGGGTTCGACTTTGCACGAAATCGTAGGGCAGATAAGGCGTCCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGC                                                               <  SRR4302518.10187189/91‑1 (MQ=60)
                                          nnncttcCTGGCCTCCGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCC                                                              <  SRR4302518.10090776/94‑1 (MQ=60)
                                           CCCGTCCTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCTTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCG                                                             <  SRR4302518.1685783/101‑1 (MQ=60)
                                           CCCGTCCTGGCCTACGGTTCGAATTGGCACGAAATCGAAGGGCAGATAGGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCG                                                             <  SRR4302518.5068698/101‑1 (MQ=60)
                                           CCCGTACTGGCCTACGGTTCGAATTTGCCCGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCG                                                             <  SRR4302518.7158710/101‑1 (MQ=60)
                                            ctgttCTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGTCAGATAAGGCTTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGG                                                            <  SRR4302518.1222609/96‑1 (MQ=60)
                                            CCGTCCTGGCCTACGGTTGGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGG                                                            <  SRR4302518.832652/101‑1 (MQ=60)
                                            CCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACTCCTTGCCTAACATAAAAGTGCCGG                                                            >  SRR4302518.4076858/1‑101 (MQ=60)
                                             NNNACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGACTGATAGGGCGTTCACGC‑‑CGTGTCTGGCACCGACCGCCTTGCCTGACATAAAAGTGCCGGA                                                           <  SRR4302518.14657728/101‑1 (MQ=58)
                                             CGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCNNN                                                           >  SRR4302518.14682592/1‑101 (MQ=60)
                                             CGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCNNN                                                           >  SRR4302518.12281152/1‑101 (MQ=60)
                                             CGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGA                                                           >  SRR4302518.2893401/1‑101 (MQ=60)
                                              gaactggcctagggttaaaattggcacaacATCGTAGGGCAGATAAGCCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAG                                                          <  SRR4302518.50527/71‑1 (MQ=60)
                                              NNNCTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAG                                                          <  SRR4302518.14394617/101‑1 (MQ=60)
                                              GTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGGAACGAACGCCTTGCCTGACATAAAAGTGCCGGAG                                                          >  SRR4302518.7001056/1‑101 (MQ=60)
                                              GTACTGGCCTACGGTTCGAATTTGCACGAAATCGTA‑GGCAGATAAGGCGGTCACGC‑‑CGCATCTGGCCACGAATGCCTTGCCTGACATAAAAGGGCCGGNNN                                                         >  SRR4302518.10594957/1‑101 (MQ=60)
                                               TACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGNNN                                                         >  SRR4302518.8402289/1‑101 (MQ=60)
                                                CCTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAA                                                        <  SRR4302518.6573454/101‑1 (MQ=60)
                                                ACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAA                                                        >  SRR4302518.4537425/1‑101 (MQ=60)
                                                 CTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGNNN                                                       >  SRR4302518.9312830/1‑101 (MQ=60)
                                                 CTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAAT                                                       <  SRR4302518.281/101‑1 (MQ=60)
                                                 CTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAAT                                                       >  SRR4302518.3328172/1‑101 (MQ=60)
                                                 CTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAAT                                                       >  SRR4302518.691454/1‑101 (MQ=60)
                                                  TGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGANNN                                                      >  SRR4302518.7657198/1‑101 (MQ=60)
                                                   NNNCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATAT                                                     <  SRR4302518.10932047/101‑1 (MQ=60)
                                                     CCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCT                                                   >  SRR4302518.3256610/1‑101 (MQ=60)
                                                     CCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCT                                                   >  SRR4302518.1361285/1‑101 (MQ=60)
                                                     CCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATANNN                                                   >  SRR4302518.9086049/1‑101 (MQ=60)
                                                      CTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATNNN                                                  >  SRR4302518.8508309/1‑101 (MQ=60)
                                                      CTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTC                                                  >  SRR4302518.3538105/1‑101 (MQ=60)
                                                      CTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTC                                                  >  SRR4302518.4142958/1‑101 (MQ=60)
                                                      CTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTC                                                  <  SRR4302518.4214472/101‑1 (MQ=60)
                                                       TACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCC                                                 >  SRR4302518.6783812/1‑101 (MQ=60)
                                                       TACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCC                                                 <  SRR4302518.6139380/101‑1 (MQ=60)
                                                        CCGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCG                                                <  SRR4302518.4517184/101‑1 (MQ=60)
                                                        ACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTNNN                                                >  SRR4302518.14816683/1‑101 (MQ=60)
                                                        ACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTNNN                                                >  SRR4302518.10703945/1‑101 (MQ=60)
                                                         NNNTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGG                                               <  SRR4302518.8738137/101‑1 (MQ=60)
                                                         CGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGG                                               <  SRR4302518.4788932/101‑1 (MQ=60)
                                                          GGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCNNN                                              >  SRR4302518.14833760/1‑101 (MQ=60)
                                                          GGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCNNN                                              >  SRR4302518.11696725/1‑101 (MQ=60)
                                                          GGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGC                                              >  SRR4302518.580285/1‑101 (MQ=60)
                                                            TTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGggagaatctccgcnnn                                            >  SRR4302518.10341581/1‑85 (MQ=60)
                                                             TCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATT                                           >  SRR4302518.3269708/1‑101 (MQ=60)
                                                             TCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATT                                           >  SRR4302518.1885970/1‑101 (MQ=60)
                                                              nnnattttaccaaaatggtagggaatgataggtgtccccgC‑‑CGCATCGGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTT                                          <  SRR4302518.7420266/61‑1 (MQ=60)
                                                              CGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTT                                          >  SRR4302518.1693659/1‑101 (MQ=60)
                                                               nnnttggcgcgaaatcggagggcaaaaagggcgtccacgC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTT                                         <  SRR4302518.10130200/62‑1 (MQ=60)
                                                               GAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTT                                         >  SRR4302518.2318635/1‑101 (MQ=60)
                                                               GAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTT                                         >  SRR4302518.1757577/1‑101 (MQ=60)
                                                               GAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATNNN                                         >  SRR4302518.11507230/1‑101 (MQ=60)
                                                                AATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTT                                        <  SRR4302518.5398151/101‑1 (MQ=60)
                                                                AATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATATCCGGCCTTTTT                                        >  SRR4302518.2573419/1‑101 (MQ=60)
                                                                 ATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTNNN                                       >  SRR4302518.11369551/1‑101 (MQ=60)
                                                                 AATTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTNNN                                       >  SRR4302518.14962991/1‑101 (MQ=60)
                                                                  TTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTNNN                                      >  SRR4302518.14007781/1‑101 (MQ=60)
                                                                   TTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATT                                     <  SRR4302518.5787271/101‑1 (MQ=60)
                                                                   TTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATT                                     >  SRR4302518.434812/1‑101 (MQ=60)
                                                                   TTGCACGAAATCGTAGGGCAGATAAGGCGGTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTTACATAAAAGTGCCGGAGGATATCTCCGGgctttttttt                                     >  SRR4302518.4227576/1‑91 (MQ=60)
                                                                   NNNCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATT                                     <  SRR4302518.7421963/101‑1 (MQ=60)
                                                                     GCACGAACTCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGACCGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCC                                   <  SRR4302518.7102213/101‑1 (MQ=60)
                                                                     GCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGGGCCGGAGAATATCTCCGGCgcttttatnnn                                   >  SRR4302518.12980374/1‑90 (MQ=60)
                                                                       CCGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCAC                                 <  SRR4302518.4005505/101‑1 (MQ=60)
                                                                       ACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCNNN                                 >  SRR4302518.14403407/1‑101 (MQ=60)
                                                                       ACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCAC                                 >  SRR4302518.3805759/1‑101 (MQ=60)
                                                                        CGAAATCGTAGGGCAGATAAGGCGTCCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACA                                <  SRR4302518.126211/101‑1 (MQ=60)
                                                                        CGAAATCGTAGGGCAGATAAGGCGGTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCGGGAGAATATCTCCGGCATTTTTATTCCACA                                >  SRR4302518.2202781/1‑101 (MQ=60)
                                                                        CGAAATCGTAGGCTAGATAAGGCGTCCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACA                                <  SRR4302518.5155060/101‑1 (MQ=60)
                                                                         NNNATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAG                               <  SRR4302518.7610655/101‑1 (MQ=60)
                                                                         NNNATCGAAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAG                               <  SRR4302518.10230069/101‑1 (MQ=60)
                                                                         GAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAG                               >  SRR4302518.971129/1‑101 (MQ=60)
                                                                         GAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAG                               <  SRR4302518.2349114/101‑1 (MQ=60)
                                                                         GAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAG                               >  SRR4302518.2716766/1‑101 (MQ=60)
                                                                          NNNTCGTAGGGCAGATAAGGCGTTCA‑‑CGACGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGC                              <  SRR4302518.10598805/101‑1 (MQ=60)
                                                                          NNNTCGAAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGC                              <  SRR4302518.13480731/101‑1 (MQ=60)
                                                                          AAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGC                              >  SRR4302518.6608786/1‑101 (MQ=60)
                                                                          AAATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGC                              >  SRR4302518.6416224/1‑101 (MQ=60)
                                                                            NNNGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATGAAACTTCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCA                            <  SRR4302518.9777804/101‑1 (MQ=60)
                                                                            NNNGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGCGAATATCTCCGGCATTTTTATTCCACAGCCA                            <  SRR4302518.12771520/101‑1 (MQ=60)
                                                                            ATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCA                            <  SRR4302518.2084324/101‑1 (MQ=60)
                                                                            ATCGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCA                            <  SRR4302518.4464432/101‑1 (MQ=60)
                                                                             nnngagggaAGATAAGGCGTTCACGC‑‑CGCAGCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAA                           <  SRR4302518.12084626/92‑1 (MQ=60)
                                                                              NNNAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAA                          <  SRR4302518.8549640/101‑1 (MQ=60)
                                                                              CGTAGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCCTTTTTATTCCACAGCCNNN                          >  SRR4302518.11348287/1‑101 (MQ=60)
                                                                              CGTAGGGCAGAGAAGGCGTCCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAA                          <  SRR4302518.651209/101‑1 (MQ=60)
                                                                               nnntgaCAGATAATGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAAC                         <  SRR4302518.8452274/95‑1 (MQ=60)
                                                                               NNNGGGCAGATAAGGCGTGCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAAC                         <  SRR4302518.11546407/101‑1 (MQ=60)
                                                                                 AGGGCAGATACGGCGTTCA‑‑CGACGCTTCTGCCAACGAACGCCTTCCCTGACATAAAAGTGCCGGAGAATATCTTCGTAATTTTTATTCCACAGCCAACCTC                       <  SRR4302518.3679733/101‑1 (MQ=56)
                                                                                 AGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAANNN                       >  SRR4302518.9352980/1‑101 (MQ=60)
                                                                                 AGGGCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAANNN                       >  SRR4302518.9921753/1‑101 (MQ=60)
                                                                                  GGGCAGATAAGGCGTCCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCA                      <  SRR4302518.1775053/101‑1 (MQ=60)
                                                                                    GCAGATACGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATA                    <  SRR4302518.4353067/101‑1 (MQ=60)
                                                                                    GCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCNNN                    >  SRR4302518.8866238/1‑101 (MQ=60)
                                                                                    GCAGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATA                    >  SRR4302518.5710992/1‑101 (MQ=60)
                                                                                      nnntaaggcgtctacgC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAAT                  <  SRR4302518.9552121/85‑1 (MQ=60)
                                                                                      AGATAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCACACTCATNNN                  >  SRR4302518.12189977/1‑101 (MQ=60)
                                                                                        ctaaggcgtccacgC‑‑CGCATCTGGCACCGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAACCTCATAATAT                <  SRR4302518.2695940/87‑1 (MQ=60)
                                                                                         AAAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATA               <  SRR4302518.4388675/101‑1 (MQ=60)
                                                                                          nnngctttcacgC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATAT              <  SRR4302518.12550039/89‑1 (MQ=60)
                                                                                          AAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGGGGATATTTCCGGCATTTTTATTCCACAGCCAAACTCCTAATANNN              >  SRR4302518.10060952/1‑101 (MQ=60)
                                                                                          AAGGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCGTTTTTTTTCCACAGCCAAACTCATAATATAT              >  SRR4302518.29488/1‑101 (MQ=60)
                                                                                            GGCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTC            <  SRR4302518.4575614/101‑1 (MQ=60)
                                                                                             GCGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGGGCGGGGATATCTCCGGCCTTTTTATTGCACAGCCCAACTCATAATATATTTC           >  SRR4302518.2695301/1‑101 (MQ=48)
                                                                                              nnnccacgC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCG          <  SRR4302518.10010828/93‑1 (MQ=60)
                                                                                              CGTTCACGC‑‑CGCATGTGGCAGCGAACGCCTTGCCTGACATAAAAGTGTCGGAGAATATTTCCGGCATTTTTATTCCACGGCCAAAGTCGTATTATGTTCCG          >  SRR4302518.2929362/1‑101 (MQ=56)
                                                                                              CGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTNNN          >  SRR4302518.10337046/1‑101 (MQ=60)
                                                                                              CGTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCG          >  SRR4302518.3626716/1‑101 (MQ=60)
                                                                                               GTTCACGC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGG         >  SRR4302518.3071883/1‑101 (MQ=60)
                                                                                                 NNN‑‑C‑‑CGCATCTGGCAACGAACGCCTTGCATGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAAT     <  SRR4302518.10567739/101‑1 (MQ=60)
                                                                                                  cacgC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGCGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATAATCCGGNNN      >  SRR4302518.14224074/5‑101 (MQ=60)
                                                                                                  cacgC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCTCAGCCAAACTCATAATATATTCCGGNNN      >  SRR4302518.10550058/5‑101 (MQ=60)
                                                                                                   acgC‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAAT     <  SRR4302518.2427583/98‑1 (MQ=60)
                                                                                                      N‑‑NNCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATT  <  SRR4302518.7898575/101‑1 (MQ=60)
                                                                                                      |                                                                                                      
GATGGATGCGCTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCA‑‑C‑‑CGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATT  >  NC_000913/3537305‑3537505

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑