Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A0 F0 I1 R1 245 579.6 28867798 97.7% 28203838 96.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,115,116 +C coding (2013/2028 nt) b2974 ← putative endoglucanase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,115,1151.C99.5% 2575.7 / 0.3 651coding (2014/2028 nt)b2974putative endoglucanase
Reads supporting (aligned to +/- strand):  ref base . (0/3);  new base C (247/400);  total (248/403)
Fisher's exact test for biased strand distribution p-value = 2.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 703 total aligned reads displayed.

TCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TT‑CTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGT  >  NC_000913/3115020‑3115205
                                                                                                 |                                                                                          
tCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAt‑tt                                                                                             >  1:4436982/1‑96 (MQ=255)
 cGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTGAAGGATCAGCAGCGGCAT‑TT‑c                                                                                           >  2:1700046/1‑96 (MQ=255)
   gTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TT‑cct                                                                                         <  2:12565400/96‑3 (MQ=255)
   gTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TT‑cct                                                                                         <  2:4406643/96‑3 (MQ=255)
    tttGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TT‑cctg                                                                                        <  2:951754/96‑4 (MQ=255)
     ttGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TT‑cctgc                                                                                       <  1:857299/96‑5 (MQ=255)
     ttGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TT‑cctgc                                                                                       <  2:583794/96‑5 (MQ=255)
       gCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCgg                                                                                      >  2:13138372/1‑96 (MQ=255)
       gCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCgg                                                                                      <  1:6284109/96‑1 (MQ=255)
        cTGGTCTCTGCACGCAGCGGGATTGCATAAGGATCGTTTCCCACCTGAGTCAAATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGa                                                                                     <  1:6001969/96‑1 (MQ=255)
        cTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGa                                                                                     >  1:7198969/1‑96 (MQ=255)
          ggaaTCTGCACGCAGCGGGATTGCATAAGGATCGTTAGCCACATGAGTCACATATTCAAAGCCGTTAAGGATGAGCAGCGGCAT‑TTCCTGCGGATc                                                                                   >  1:12393402/4‑96 (MQ=255)
          ggTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATc                                                                                   <  2:7151137/96‑1 (MQ=255)
          ggTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATc                                                                                   <  2:7327234/96‑1 (MQ=255)
             aTCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCgag                                                                                <  1:3148773/96‑1 (MQ=255)
               cagCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGGCACATATTCAAAGCCGTTAAGGAGCAGCAGCGGCATGTC‑CTGCGGATCGAGAt                                                                              >  1:3560903/3‑96 (MQ=255)
                tGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATc                                                                             <  2:5990699/96‑1 (MQ=255)
                tGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATc                                                                             >  1:7286218/1‑96 (MQ=255)
                tGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATc                                                                             <  2:1959694/96‑1 (MQ=255)
                tGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATc                                                                             <  1:2720451/96‑1 (MQ=255)
                tGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATc                                                                             <  2:10367577/96‑1 (MQ=255)
                   aCGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGcc                                                                          <  1:2957675/96‑1 (MQ=255)
                    cGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCa                                                                         <  2:9061208/96‑1 (MQ=255)
                    cGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCa                                                                         >  1:10895738/1‑96 (MQ=255)
                     gCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCAt                                                                        <  1:1952025/96‑1 (MQ=255)
                      cAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCTGCAT‑TTCCTTCGGATCGAGATCGCCATa                                                                       <  1:13205743/96‑1 (MQ=255)
                      cAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATa                                                                       >  1:2229369/1‑96 (MQ=255)
                      cAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATa                                                                       <  1:10014663/96‑1 (MQ=255)
                       aGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATTTAGATCTCCATAg                                                                      <  1:13440659/96‑1 (MQ=255)
                       aGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAg                                                                      >  1:1364457/1‑96 (MQ=255)
                       aGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAg                                                                      <  2:12848135/96‑1 (MQ=255)
                        gCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATaac                                                                     <  1:10619954/96‑3 (MQ=255)
                          gggATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTa                                                                   <  1:9197495/96‑1 (MQ=255)
                            gATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACt                                                                 <  1:8982849/96‑1 (MQ=255)
                             aTTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACtt                                                                <  2:10706520/96‑1 (MQ=255)
                              ttGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTa                                                               <  2:10083885/96‑1 (MQ=255)
                              ttGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTa                                                               <  1:8329314/96‑1 (MQ=255)
                              ttGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTa                                                               <  1:7012386/96‑1 (MQ=255)
                                 cATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAAt                                                            >  2:8627210/1‑96 (MQ=255)
                                 cATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAAt                                                            <  1:6595215/96‑1 (MQ=255)
                                  aTAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATg                                                           >  2:8831049/1‑96 (MQ=255)
                                   tAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGc                                                          <  2:2231416/96‑1 (MQ=255)
                                    aaGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCt                                                         >  1:4613718/1‑96 (MQ=255)
                                     aGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTc                                                        >  1:14409529/1‑96 (MQ=255)
                                        aTCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAAc                                                     >  2:10594163/1‑96 (MQ=255)
                                        aTCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAAc                                                     >  2:640888/1‑96 (MQ=255)
                                         tCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACa                                                    <  2:6147304/96‑1 (MQ=255)
                                          cGTGACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACag                                                   <  1:6749635/96‑1 (MQ=255)
                                           gTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACaga                                                  >  2:2975138/1‑96 (MQ=255)
                                           gTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACaga                                                  <  1:5015680/96‑1 (MQ=255)
                                            ttACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACagag                                                 <  2:5647498/96‑1 (MQ=255)
                                            ttACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACagag                                                 <  2:14344291/96‑1 (MQ=255)
                                             tACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAgaga                                                >  1:4211328/1‑96 (MQ=255)
                                             tACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAgaga                                                <  2:13375115/96‑1 (MQ=255)
                                             tACCCACCTGAGTCACATATTCAAAGCCGTTAAAGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAgaga                                                >  2:14080800/1‑96 (MQ=255)
                                               cccACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATg                                              <  2:12312393/96‑1 (MQ=255)
                                                ccACCTGAGTCACATAGTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGAttc                                             >  1:4025786/1‑94 (MQ=255)
                                                 cACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGcc                                            <  1:13924991/96‑1 (MQ=255)
                                                  aCCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGccc                                           <  2:2605740/96‑1 (MQ=255)
                                                  aCCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCAGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGccc                                           <  2:11154017/96‑1 (MQ=255)
                                                   ccTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCg                                          >  1:10350853/1‑96 (MQ=255)
                                                    cTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGc                                         >  2:4072189/1‑96 (MQ=255)
                                                            caTATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATTGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCg                                 <  1:13026227/96‑1 (MQ=255)
                                                            caTATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCg                                 <  1:11520338/96‑1 (MQ=255)
                                                              tatTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGa                               >  2:7581497/1‑96 (MQ=255)
                                                               atTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGAt                              <  2:9641835/96‑1 (MQ=255)
                                                               atTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGAt                              <  2:12012056/96‑1 (MQ=255)
                                                                 tCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATgg                            <  2:6756607/96‑1 (MQ=255)
                                                                  cAAAGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGa                           >  1:5301245/1‑96 (MQ=255)
                                                                   aaaGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGaa                          <  1:11818710/96‑1 (MQ=255)
                                                                   aaaGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGaa                          <  2:9324020/96‑1 (MQ=255)
                                                                   aaaGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGAGGGaa                          >  2:7004208/1‑96 (MQ=255)
                                                                    aaGCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAg                         <  1:14138492/96‑1 (MQ=255)
                                                                      gCCGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTc                       <  2:11451342/96‑1 (MQ=255)
                                                                        cGTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGa                     <  2:4970980/96‑1 (MQ=255)
                                                                         gTTAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGaa                    <  2:1364457/96‑1 (MQ=255)
                                                                          ttAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAAc                   <  1:11116996/96‑1 (MQ=255)
                                                                          ttAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAAc                   >  2:3506462/1‑96 (MQ=255)
                                                                          ttAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAAc                   <  2:11763733/96‑1 (MQ=255)
                                                                          ttAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAAc                   <  1:6458078/96‑1 (MQ=255)
                                                                           tAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACg                  <  1:8022627/96‑1 (MQ=255)
                                                                           tAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACg                  >  1:7628524/1‑96 (MQ=255)
                                                                           tAAGGATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACg                  <  2:815124/96‑1 (MQ=255)
                                                                            aatgATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGc                 >  2:2434743/4‑96 (MQ=255)
                                                                               gATCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAgc              <  2:8380445/96‑1 (MQ=255)
                                                                                aTCAGCAGCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAgcg             >  2:5246295/1‑96 (MQ=255)
                                                                                  cagcagCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAgcgca           <  1:11374148/96‑2 (MQ=255)
                                                                                  cagcagCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCt           <  2:3270440/96‑1 (MQ=255)
                                                                                  cagcagCGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCt           <  1:3352245/96‑1 (MQ=255)
                                                                                   agcagcGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGGCGAACGCAGCGCTg          >  1:9533697/1‑96 (MQ=255)
                                                                                    gcagcGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTgn         <  1:5659250/96‑2 (MQ=255)
                                                                                    gcagcGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGt         <  1:4766549/96‑1 (MQ=255)
                                                                                    gcagcGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGt         >  2:4716594/1‑96 (MQ=255)
                                                                                      agcGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGttt       >  1:2584646/1‑96 (MQ=255)
                                                                                      agcGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGttt       >  1:9710978/1‑96 (MQ=255)
                                                                                      agcGGCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGAGGGAAGTCGAACGCAGCGCTGttt       >  2:3880407/1‑96 (MQ=255)
                                                                                       gcGGCAT‑TTCCTGCGGATCGAGATCGCCATCGCTACGTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTc      <  1:12714983/96‑1 (MQ=255)
                                                                                       gcGGCAT‑TTCCTACGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATTCCCGCAAAATCCGGATGGAAGGCGAACGCAGCGCTGTTTc      <  1:12042575/96‑1 (MQ=255)
                                                                                        cGGCAT‑ATCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTcc     >  1:2473523/1‑96 (MQ=255)
                                                                                          gCAT‑TTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGCGATGCCCGCAAACTCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTg   <  1:1736358/96‑1 (MQ=255)
                                                                                                 |                                                                                          
TCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAT‑TT‑CTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGT  >  NC_000913/3115020‑3115205

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 433 total aligned reads displayed.
AGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTT‑CTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGTAACCTGACCAT  >  NC_000913/3115016‑3115216
                                                                                                     |                                                                                                    
ATCTTCGGCTTGCTGGTATCTGCACGCCGCGGGATTGCAGAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCACAAGCGGCATNN‑N                                                                                                      >  SRR4302518.11054937/1‑101 (MQ=60)
AGCTTCGGTTTGCTGGTATCTGTACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTT‑C                                                                                                      >  SRR4302518.2687120/1‑101 (MQ=60)
AGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATNN‑N                                                                                                      >  SRR4302518.10312727/1‑101 (MQ=60)
 GCTTCGGTTTGCTGGGATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTT‑CC                                                                                                     >  SRR4302518.2217709/1‑101 (MQ=60)
  NNNCGGTCTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTT‑Cct                                                                                                    <  SRR4302518.9647661/101‑3 (MQ=60)
   TTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTT‑Cctg                                                                                                   >  SRR4302518.4811642/1‑98 (MQ=60)
   TTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTT‑Cctg                                                                                                   >  SRR4302518.6816286/1‑98 (MQ=60)
    TCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGC                                                                                                   <  SRR4302518.890436/101‑1 (MQ=60)
    TCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGC                                                                                                   <  SRR4302518.262410/101‑1 (MQ=60)
    NNNGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGC                                                                                                   <  SRR4302518.12990915/101‑1 (MQ=60)
     NNNTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCCCCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTT‑Cctgca                                                                                                 <  SRR4302518.11930640/101‑6 (MQ=60)
     NNNTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCG                                                                                                  <  SRR4302518.13175143/101‑1 (MQ=60)
     CGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCG                                                                                                  <  SRR4302518.4429283/101‑1 (MQ=60)
      GGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGG                                                                                                 <  SRR4302518.6604406/101‑1 (MQ=60)
       GTTTGCTGGTCTCTGCACGCAGCGGGATTGCATAAGGATCGTTTCCCACCTGAGTCAAATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGA                                                                                                <  SRR4302518.6306469/101‑1 (MQ=60)
        TTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCCCCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGAT                                                                                               <  SRR4302518.6929615/101‑1 (MQ=60)
        TTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAGAGCCGTTAAGGATCAGCAGCGGCATTT‑Ccagcggat                                                                                              >  SRR4302518.5116022/1‑93 (MQ=60)
        NNNGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGAT                                                                                               <  SRR4302518.10126660/101‑1 (MQ=60)
         nnnatggtgttTGCACGCAGCGGGATTGCATAAAGATCGTTACCCACCTGAGGCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATC                                                                                              <  SRR4302518.12362459/90‑1 (MQ=60)
         NNNCTGGTATCTGCACGCAGCGTGATTGAATAAGGATCTTTACCCACCTGATTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATC                                                                                              <  SRR4302518.12913456/101‑1 (MQ=60)
          TGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCG                                                                                             <  SRR4302518.6766478/101‑1 (MQ=60)
          TGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGANNN                                                                                             >  SRR4302518.13711157/1‑101 (MQ=60)
           NNNGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCACCGGCATTTCCTGCGGATCGA                                                                                            <  SRR4302518.9364907/101‑1 (MQ=60)
           GCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGA                                                                                            <  SRR4302518.3703471/101‑1 (MQ=60)
            CTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCNNN                                                                                           >  SRR4302518.7576401/1‑101 (MQ=60)
            CTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAG                                                                                           <  SRR4302518.5040160/101‑1 (MQ=60)
            CTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAG                                                                                           <  SRR4302518.3295967/101‑1 (MQ=60)
             TGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGGTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGA                                                                                          >  SRR4302518.7034108/1‑101 (MQ=60)
             NNNTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGA                                                                                          <  SRR4302518.13366288/101‑1 (MQ=60)
              NNNATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGTT                                                                                         <  SRR4302518.12033060/101‑1 (MQ=60)
              GGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGAT                                                                                         <  SRR4302518.1342718/101‑1 (MQ=60)
               GTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATC                                                                                        <  SRR4302518.2849781/101‑1 (MQ=60)
               GTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATC                                                                                        <  SRR4302518.3270902/101‑1 (MQ=60)
                NNNCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCG                                                                                       <  SRR4302518.7958734/101‑1 (MQ=60)
                NNNCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCG                                                                                       <  SRR4302518.10955454/101‑1 (MQ=60)
                 ATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATNNN                                                                                      >  SRR4302518.13776271/1‑101 (MQ=60)
                 ATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGC                                                                                      <  SRR4302518.7248266/101‑1 (MQ=60)
                  TCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCC                                                                                     <  SRR4302518.6937888/101‑1 (MQ=60)
                  TCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCC                                                                                     <  SRR4302518.3096366/101‑1 (MQ=60)
                  NNNGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGTC                                                                                     <  SRR4302518.14969679/101‑1 (MQ=60)
                  NNNGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCC                                                                                     <  SRR4302518.14668711/101‑1 (MQ=60)
                  NNNGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCC                                                                                     <  SRR4302518.14111409/101‑1 (MQ=60)
                    cacaaggAGCGGGATTGCATAAGGATCGTGACCCACCTGAGTCAAATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTATTGCGGATCGAGATCGCAAT                                                                                   <  SRR4302518.358/94‑1 (MQ=60)
                    NNNACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCAT                                                                                   <  SRR4302518.8800769/101‑1 (MQ=60)
                     NNNCGCAGCGGGATTGTATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCTGCATTTCCTTCGGATCGAGATCGCCATA                                                                                  <  SRR4302518.13814960/101‑1 (MQ=60)
                     GCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATA                                                                                  <  SRR4302518.949602/101‑1 (MQ=60)
                      CACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAG                                                                                 >  SRR4302518.5049126/1‑101 (MQ=60)
                      CACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATAGTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCANNN                                                                                 >  SRR4302518.14872497/1‑101 (MQ=60)
                      AACGCAGCGGGATTGCAACATGATCGTTACCCGCCGGAGTAACATATTCAGAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAG                                                                                 <  SRR4302518.113326/101‑1 (MQ=60)
                       NNNCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAAC                                                                                <  SRR4302518.11119025/101‑1 (MQ=60)
                       ACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGC                                                                                >  SRR4302518.4006711/1‑101 (MQ=60)
                        CGCAGCGGGATTGCATAAGGATCGTTATCGACCTGAGTCACATATTCAAAGCCGCTAAGGATCAGCACCGGCATTT‑Cctgcggagagagattaccatannn                                                                              >  SRR4302518.12642519/1‑77 (MQ=60)
                        CGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCT                                                                               >  SRR4302518.5514448/1‑101 (MQ=60)
                        CGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCT                                                                               <  SRR4302518.27702/101‑1 (MQ=60)
                         NNNGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTA                                                                              <  SRR4302518.9641112/101‑1 (MQ=60)
                         GCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTA                                                                              <  SRR4302518.4219842/101‑1 (MQ=60)
                          CAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTAC                                                                             <  SRR4302518.7312760/101‑1 (MQ=60)
                          CAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTAC                                                                             >  SRR4302518.2336962/1‑101 (MQ=60)
                          CAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTAC                                                                             >  SRR4302518.3327479/1‑101 (MQ=60)
                           NNNGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACT                                                                            <  SRR4302518.14003506/101‑1 (MQ=60)
                           NNNGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACT                                                                            <  SRR4302518.7521659/101‑1 (MQ=60)
                           NNNGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACT                                                                            <  SRR4302518.9417127/101‑1 (MQ=60)
                           AGCGGGATTGCATGAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTNNN                                                                            >  SRR4302518.11489907/1‑101 (MQ=60)
                            GCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTT                                                                           <  SRR4302518.4728783/101‑1 (MQ=60)
                            GCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTT                                                                           <  SRR4302518.6872163/101‑1 (MQ=60)
                             NNNGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTA                                                                          <  SRR4302518.7384346/101‑1 (MQ=60)
                             NNNGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTA                                                                          <  SRR4302518.8738949/101‑1 (MQ=60)
                              GGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAA                                                                         >  SRR4302518.2392286/1‑101 (MQ=60)
                              GGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTNNN                                                                         >  SRR4302518.13986349/1‑101 (MQ=60)
                              GGGATTGCATAAGGATCGTTACCCACCCGAGCCACATATTCAAAGCCGTTAAGGATCAGAAGCGGCATTTCCTGCGGAGCGAGATCaacagcgctagttaa                                                                         >  SRR4302518.4661054/1‑86 (MQ=60)
                               NNNTTGCATAAGGATCGTTACCCACCTGGGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAA                                                                        <  SRR4302518.10586370/101‑1 (MQ=60)
                                 ATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAANNN                                                                      >  SRR4302518.8400863/1‑101 (MQ=60)
                                  TTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAANNN                                                                     >  SRR4302518.10150918/1‑101 (MQ=60)
                                  NNNCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGT                                                                     <  SRR4302518.14525254/101‑1 (MQ=60)
                                   TGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCT                                                                    <  SRR4302518.7172994/101‑1 (MQ=60)
                                   NNNATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCT                                                                    <  SRR4302518.8488848/101‑1 (MQ=60)
                                   NNNATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCT                                                                    <  SRR4302518.9710531/101‑1 (MQ=60)
                                    GCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTC                                                                   <  SRR4302518.4940067/101‑1 (MQ=60)
                                    GAATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCCGCGGATCGAGATCGCCATAGCTACTTAAATGCTC                                                                   <  SRR4302518.2727019/101‑1 (MQ=60)
                                     CATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCA                                                                  <  SRR4302518.4767782/101‑1 (MQ=60)
                                     CATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCA                                                                  >  SRR4302518.1859524/1‑101 (MQ=60)
                                     CATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCA                                                                  >  SRR4302518.1502582/1‑101 (MQ=60)
                                     CATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCA                                                                  >  SRR4302518.5863631/1‑101 (MQ=60)
                                     CATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCA                                                                  <  SRR4302518.554667/101‑1 (MQ=60)
                                       TAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAAC                                                                <  SRR4302518.2991021/101‑1 (MQ=60)
                                       TAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAAC                                                                <  SRR4302518.3631386/101‑1 (MQ=60)
                                       TAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAAC                                                                <  SRR4302518.6555884/101‑1 (MQ=60)
                                       NNNGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAAC                                                                <  SRR4302518.12447245/101‑1 (MQ=60)
                                        NNNGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACG                                                               <  SRR4302518.14461518/101‑1 (MQ=60)
                                        AAGGATCGTTACCCCCCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACA                                                               <  SRR4302518.1484546/101‑1 (MQ=60)
                                        AAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGGGATCGCCATAGTTACTTAAATGCTCAACA                                                               >  SRR4302518.1041469/1‑101 (MQ=60)
                                        AAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGTTCANNN                                                               >  SRR4302518.10259852/1‑101 (MQ=60)
                                        AAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACA                                                               >  SRR4302518.4992421/1‑101 (MQ=60)
                                         AGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAANNN                                                              >  SRR4302518.15078704/1‑101 (MQ=60)
                                         AGGATCGTGACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAG                                                              <  SRR4302518.7101793/101‑1 (MQ=60)
                                          GGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGA                                                             <  SRR4302518.5276015/101‑1 (MQ=60)
                                          GGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGAGGCATTTCCTGCGGATCGATAGCGACATAGCTACTTAAATGCTCAAaagc                                                             >  SRR4302518.5624665/1‑97 (MQ=60)
                                           NNNCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAG                                                            <  SRR4302518.9085985/101‑1 (MQ=60)
                                           NNNCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAG                                                            <  SRR4302518.8289342/101‑1 (MQ=60)
                                           GATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAG                                                            <  SRR4302518.7069643/101‑1 (MQ=60)
                                             nnnttcCCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGAT                                                          <  SRR4302518.12170458/95‑1 (MQ=60)
                                             TCGTTCCCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGAT                                                          <  SRR4302518.2597074/101‑1 (MQ=60)
                                             TCGTTACCCACCTGAGTCACATATGCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGAT                                                          <  SRR4302518.6061169/101‑1 (MQ=60)
                                              NNNTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATG                                                         <  SRR4302518.14626467/101‑1 (MQ=60)
                                              CGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATTGCCATAGCTACTTAAATGCTCAACAGAGATG                                                         >  SRR4302518.6744233/1‑101 (MQ=60)
                                              CGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCGACAGAGNNN                                                         >  SRR4302518.8509530/1‑101 (MQ=60)
                                               GTTACCCACCTGAGTCACATCTTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGC                                                        <  SRR4302518.6574692/101‑1 (MQ=60)
                                               GTTACCCACCTGAGTAACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGC                                                        <  SRR4302518.3056528/101‑1 (MQ=60)
                                               ATTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGANNN                                                        >  SRR4302518.14455142/1‑101 (MQ=60)
                                                TTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCC                                                       <  SRR4302518.1912126/101‑1 (MQ=60)
                                                NNNCCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCC                                                       <  SRR4302518.14562203/101‑1 (MQ=60)
                                                 TACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCC                                                      >  SRR4302518.3691402/1‑101 (MQ=60)
                                                 TACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCC                                                      <  SRR4302518.2656318/101‑1 (MQ=60)
                                                 TACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGACGCCC                                                      >  SRR4302518.4417210/1‑101 (MQ=60)
                                                 NNNCCACCTGAGTCACATATTCAAAGCCGGTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTTCTTAAATGCTCAACAGAGATGCCC                                                      <  SRR4302518.8850570/101‑1 (MQ=60)
                                                     CTCCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAA                                                  <  SRR4302518.5323728/101‑1 (MQ=60)
                                                     CACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAA                                                  >  SRR4302518.4886545/1‑101 (MQ=60)
                                                      ACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAA                                                 >  SRR4302518.2971042/1‑101 (MQ=60)
                                                       CCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCANNN                                                >  SRR4302518.7878285/1‑101 (MQ=60)
                                                       CCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCANNN                                                >  SRR4302518.9798717/1‑101 (MQ=60)
                                                       CCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGATCAACAGAGATGCCCGCANNN                                                >  SRR4302518.10837625/1‑101 (MQ=60)
                                                        CTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAAT                                               >  SRR4302518.899883/1‑101 (MQ=60)
                                                          NNNTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCC                                             <  SRR4302518.10031811/101‑1 (MQ=60)
                                                          GAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCACCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAANNN                                             >  SRR4302518.9254830/1‑101 (MQ=60)
                                                           NNNCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCG                                            <  SRR4302518.12061778/101‑1 (MQ=60)
                                                           AGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCG                                            >  SRR4302518.5381630/1‑101 (MQ=60)
                                                            GTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGACCGCAAACACCGG                                           >  SRR4302518.6170085/1‑101 (MQ=60)
                                                              CACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAACTCCGGAT                                         >  SRR4302518.3385584/1‑101 (MQ=60)
                                                              CACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGNNN                                         >  SRR4302518.11373004/1‑101 (MQ=60)
                                                               NNNTATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATG                                        <  SRR4302518.7491289/101‑1 (MQ=60)
                                                               ACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATG                                        >  SRR4302518.4189022/1‑101 (MQ=60)
                                                               ACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATG                                        >  SRR4302518.5130241/1‑101 (MQ=60)
                                                                   NNNCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAGTGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAG                                    <  SRR4302518.13535591/101‑1 (MQ=60)
                                                                   NNNCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAG                                    <  SRR4302518.14781463/101‑1 (MQ=60)
                                                                   ATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAG                                    <  SRR4302518.4242384/101‑1 (MQ=60)
                                                                    TTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGT                                   <  SRR4302518.994439/101‑1 (MQ=60)
                                                                    TTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGT                                   <  SRR4302518.3908505/101‑1 (MQ=60)
                                                                    TTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGT                                   >  SRR4302518.1951688/1‑101 (MQ=60)
                                                                      CAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCG                                 <  SRR4302518.1167067/101‑1 (MQ=60)
                                                                      CAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGNNN                                 >  SRR4302518.11771177/1‑101 (MQ=60)
                                                                        NNNCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAA                               <  SRR4302518.7669800/101‑1 (MQ=60)
                                                                         NNNCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAAC                              <  SRR4302518.11637004/101‑1 (MQ=60)
                                                                         AGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGNNN                              >  SRR4302518.7597104/1‑101 (MQ=60)
                                                                         AGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAAC                              <  SRR4302518.6788590/101‑1 (MQ=60)
                                                                          NNNGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACG                             <  SRR4302518.14204020/101‑1 (MQ=60)
                                                                            CGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCA                           <  SRR4302518.1762515/101‑1 (MQ=60)
                                                                             GTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGNNN                          >  SRR4302518.13645647/1‑101 (MQ=60)
                                                                             GTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAC                          >  SRR4302518.4549660/1‑101 (MQ=60)
                                                                             GTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGAGGGAAGTCGAACGNNN                          >  SRR4302518.13676364/1‑101 (MQ=60)
                                                                             GTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGACATCGCCATAGGTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATTGAAGTCGATCGCAG                          >  SRR4302518.3812646/1‑101 (MQ=60)
                                                                              TTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCNNN                         >  SRR4302518.7731187/1‑101 (MQ=60)
                                                                              TTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGC                         <  SRR4302518.3141317/101‑1 (MQ=60)
                                                                              TTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGC                         >  SRR4302518.1538448/1‑101 (MQ=60)
                                                                              GTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGC                         <  SRR4302518.3114923/101‑1 (MQ=60)
                                                                               TAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGGTCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCG                        >  SRR4302518.2573398/1‑101 (MQ=60)
                                                                                AAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAGGTCGAACGCAGNNN                       >  SRR4302518.12540994/1‑101 (MQ=60)
                                                                                AAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGC                       >  SRR4302518.4302809/1‑101 (MQ=60)
                                                                                 NNNATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCA                      <  SRR4302518.11907063/101‑1 (MQ=60)
                                                                                 AGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCNNN                      >  SRR4302518.14061135/1‑101 (MQ=60)
                                                                                 AGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCNNN                      >  SRR4302518.10183995/1‑101 (MQ=60)
                                                                                 AGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCT                      <  SRR4302518.1341779/101‑1 (MQ=60)
                                                                                 AAGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGTAGTCGAACGCAGCNNN                      >  SRR4302518.12320170/1‑101 (MQ=60)
                                                                                   NATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGN                    <  SRR4302518.5946551/101‑1 (MQ=60)
                                                                                   GATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGAGCGGT                    >  SRR4302518.2311535/1‑101 (MQ=60)
                                                                                    NNNAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATTGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTT                   <  SRR4302518.12885254/101‑1 (MQ=60)
                                                                                    NNNAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTT                   <  SRR4302518.9515885/101‑1 (MQ=60)
                                                                                    ATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTT                   <  SRR4302518.4473973/101‑1 (MQ=60)
                                                                                    AACAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGGTACTTAAATGCTCAACAGAGATGCCCGCAAAAGCCGGATGGAAGTCGAACGCAGCGCTNNN                   >  SRR4302518.10629861/1‑101 (MQ=60)
                                                                                     TCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGNNN                  >  SRR4302518.12721955/1‑101 (MQ=60)
                                                                                     TCAGCAGCGGCATTTCCTGCGGATCGAGATAGCCATAGCTACTTAAATGCTCAGCAGAGATGCCAGCAAAATCCGGATGGAAGTCGAACTCAGCGCTGNNN                  >  SRR4302518.8373399/1‑101 (MQ=60)
                                                                                     NNNGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTT                  <  SRR4302518.14179961/101‑1 (MQ=60)
                                                                                      CAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGCTGGAAGTCGAACGCAGCGCTGTNNN                 >  SRR4302518.11351888/1‑101 (MQ=60)
                                                                                      CAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTC                 >  SRR4302518.1051149/1‑101 (MQ=60)
                                                                                      CAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTNNN                 >  SRR4302518.7784848/1‑101 (MQ=60)
                                                                                      CAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTNNN                 >  SRR4302518.11583595/1‑101 (MQ=60)
                                                                                       AGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGGACGCAGCGCTGTTTCC                >  SRR4302518.2409387/1‑101 (MQ=60)
                                                                                        NNNGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCT               <  SRR4302518.7646247/101‑1 (MQ=60)
                                                                                        GTAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTNNN               >  SRR4302518.12406520/1‑101 (MQ=60)
                                                                                        GCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCT               <  SRR4302518.2740077/101‑1 (MQ=60)
                                                                                         CAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGCGATGCCCGCAAACTCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTG              <  SRR4302518.1813765/101‑1 (MQ=60)
                                                                                          AGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCGCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGA             >  SRR4302518.4677794/1‑101 (MQ=60)
                                                                                          AGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGT             <  SRR4302518.6022595/101‑1 (MQ=60)
                                                                                          AGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCNNN             >  SRR4302518.11530687/1‑101 (MQ=60)
                                                                                          AGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCNNN             >  SRR4302518.11979249/1‑101 (MQ=60)
                                                                                           NNNGCATTTCCTGCGGATCTAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGAA            <  SRR4302518.13887091/101‑1 (MQ=60)
                                                                                             nnnatttcCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGTAAC          <  SRR4302518.8784667/93‑1 (MQ=60)
                                                                                             GGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGTNNN          >  SRR4302518.13072639/1‑101 (MQ=60)
                                                                                             GGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGTNNN          >  SRR4302518.13236205/1‑101 (MQ=60)
                                                                                             GGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGTAAC          >  SRR4302518.2176788/1‑101 (MQ=60)
                                                                                              GCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGTANNN         >  SRR4302518.8684303/1‑101 (MQ=60)
                                                                                              GCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGTAACC         <  SRR4302518.1238717/101‑1 (MQ=60)
                                                                                               tatttcCTGCGGATCGAGATCGCTATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGTAACCT        <  SRR4302518.760902/95‑1 (MQ=60)
                                                                                                ATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGCGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGTAACCTG       <  SRR4302518.4124624/101‑1 (MQ=60)
                                                                                                ATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGTAACNNN       >  SRR4302518.8206303/1‑101 (MQ=60)
                                                                                                ATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGTAACCTG       >  SRR4302518.2495902/1‑101 (MQ=60)
                                                                                                 TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGTAACCTGA      <  SRR4302518.665778/101‑1 (MQ=60)
                                                                                                 TTC‑CTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTGCTGTAACCTGAC     >  SRR4302518.3438081/1‑101 (MQ=60)
                                                                                                  TC‑CTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGTActctgnnn    >  SRR4302518.10904532/1‑93 (MQ=60)
                                                                                                  NN‑NTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGTAACCTGAAC    <  SRR4302518.11164409/101‑1 (MQ=60)
                                                                                                     CTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGTAACCTGACCAT  >  SRR4302518.6520447/1‑101 (MQ=60)
                                                                                                     |                                                                                                    
AGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTT‑CTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGTAACCTGACCAT  >  NC_000913/3115016‑3115216

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑