Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,115,116 +C coding (2013/2028 nt) b2974 ← putative endoglucanase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,115,1151.C99.2% 825.4 / ‑1.5 254coding (2014/2028 nt)b2974putative endoglucanase
Reads supporting (aligned to +/- strand):  ref base . (1/1);  new base C (132/120);  total (133/121)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.74e-01

Only 100 of 270 total aligned reads displayed.

TTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATT‑T‑CTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCC  >  NC_000913/3115025‑3115202
                                                                                            |                                                                                       
   cTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATT‑T‑cctgc                                                                                    <  1:2340552/93‑5 (MQ=255)
     ggTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATT‑TCCTGCgg                                                                                   >  2:2876291/1‑93 (MQ=255)
      gTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATT‑TCCTGCGGa                                                                                  <  2:2139715/93‑1 (MQ=255)
      gTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATT‑TCCTGCGGa                                                                                  >  1:5030239/1‑93 (MQ=255)
       tATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATT‑TCCTGCGGAt                                                                                 >  2:2451696/1‑93 (MQ=255)
        aTCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATT‑TCCTGCGGATc                                                                                <  2:6020040/93‑1 (MQ=255)
          cTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATT‑TCCTGCGGATCga                                                                              <  1:4018374/93‑1 (MQ=255)
          cTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATT‑TCCTGCGGATCga                                                                              <  1:1724065/93‑1 (MQ=255)
          cTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATT‑TCCTGCGGATCga                                                                              <  2:2030022/93‑1 (MQ=255)
           tGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATT‑TCCTGCGGATCgag                                                                             >  2:3606315/1‑93 (MQ=255)
             cACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATT‑TCCTGCGGATCGAGAt                                                                           <  1:6116084/93‑1 (MQ=255)
                gCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATT‑TCCTGCGGATCGAGATCGc                                                                        >  1:2321184/1‑93 (MQ=255)
                 cAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATT‑TCCTGCGGATCGAGATCGcc                                                                       <  2:1769308/93‑1 (MQ=255)
                   gCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATT‑TCCTGCGGATCGAGATCGCCAt                                                                     >  2:2766397/1‑93 (MQ=255)
                   gCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATT‑TCCTGCGGATCGAGATCGCCAt                                                                     >  1:95750/1‑93 (MQ=255)
                   gCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATT‑TCCTGCGGATCGAGATCGCCAt                                                                     >  1:1143202/1‑93 (MQ=255)
                    cGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATT‑TCCTGCGGATCGAGATCGCCATa                                                                    <  2:5924182/93‑1 (MQ=255)
                    cGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATT‑TCCTGCGGATCGAGATCGCCATa                                                                    <  1:4306317/93‑1 (MQ=255)
                    agggATTGCATAAGGATCGTTACCGACGTGAGTCTCATATTCACAGCCGTTAAGGATCAGCAGCTTCATT‑TCCTGCGGATCGAGATCGCCATa                                                                    <  1:6215416/92‑1 (MQ=255)
                     gggATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATT‑TCCTGCGGATCGAGATCGCCATAg                                                                   <  2:4653031/93‑1 (MQ=255)
                     gggATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATT‑TCCTGCGGATCGAGATCGCCATAg                                                                   >  2:2926722/1‑93 (MQ=255)
                      ggATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAATGCCGTTAAGGATCAGCAGCGGCATT‑TCCTGCGGATCGAGATCGCCATAGc                                                                  >  1:3375515/1‑93 (MQ=255)
                       gATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATT‑TCCTGCGGATCGAGATCGCCATAGCt                                                                 >  2:3714469/1‑93 (MQ=255)
                         ttGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATT‑TCCTGCGGATCGAGATCGCCATAGCTAc                                                               >  2:5612456/1‑93 (MQ=255)
                         ttGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATT‑TCCTGCGGATCGAGATCGCCATAGCTAc                                                               >  1:3139620/1‑93 (MQ=255)
                         ttGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATT‑TCCTGCGGATCGAGATCGCCATAGCTAc                                                               <  1:2952845/93‑1 (MQ=255)
                         ttGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATT‑TCCTGCGGATCGAGATCGCCATAGCTAc                                                               >  2:1575949/1‑93 (MQ=255)
                            cATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATT‑TCCTGCGGATCGAGATCGCCATAGCTACTTa                                                            >  2:4842192/1‑93 (MQ=255)
                            cATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATT‑TCCTGCGGATCGAGATCGCCATAGCTACTTa                                                            >  2:1062581/1‑93 (MQ=255)
                              tAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATT‑TCCTGCGGATCGAGATCGCCATAGCTACTTaaa                                                          <  1:5542167/93‑1 (MQ=255)
                              tAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATT‑TCCTGCGGATCGAGATCGCCATAGCTACTTaaa                                                          >  1:4095397/1‑93 (MQ=255)
                              tAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATT‑TCCTGCGGATCGAGATCGCCATAGCTACTTaaa                                                          >  2:5187434/1‑93 (MQ=255)
                                aGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATT‑TCCTGCGGATCGAGATCGCCATAGCTACTTAAATg                                                        >  2:5738011/1‑93 (MQ=255)
                                  gATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATT‑TCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCt                                                      <  1:3757434/93‑1 (MQ=255)
                                   aTCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATT‑TCCTGCGGATCGAGATCGCCATAGCTACTTAAATtctc                                                     <  1:1649112/93‑1 (MQ=255)
                                   aTCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATT‑TCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTc                                                     <  1:1522283/93‑1 (MQ=255)
                                   aTCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATT‑TCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTc                                                     <  1:4841259/93‑1 (MQ=255)
                                   aTCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATT‑TCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTc                                                     >  2:5428922/1‑93 (MQ=255)
                                    tCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATT‑TCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCa                                                    >  2:407872/1‑93 (MQ=255)
                                     cGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATT‑TCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCaa                                                   <  2:5484542/93‑1 (MQ=255)
                                      gTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATT‑TCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAAc                                                  <  2:427114/93‑1 (MQ=255)
                                      gTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATT‑TCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAAc                                                  >  1:3267034/1‑93 (MQ=255)
                                          cccACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATT‑TCCTGCGGATCGAGATCGCCATAGGTACTTAAATGCTCAACagag                                              <  2:2490055/93‑1 (MQ=255)
                                           cccccTGAGCCACATATTCAAAGCCGTTACGGATCAGCAGCGGAATT‑TCCTGCGGATCGAGATCGCCATAGCTACTTCACTGCTCTACAgaga                                             >  2:463075/4‑93 (MQ=255)
                                           ccACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATT‑TCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAgaga                                             >  2:1836821/1‑93 (MQ=255)
                                            cACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATT‑TCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGAt                                            >  1:2103919/1‑93 (MQ=255)
                                            cACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATT‑TCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGAt                                            <  2:129965/93‑1 (MQ=255)
                                             cccTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATT‑TCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATg                                           <  2:2958479/92‑1 (MQ=255)
                                             aCCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATT‑TCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATg                                           >  1:5239704/1‑93 (MQ=255)
                                              ccTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATT‑TCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGc                                          >  1:5811968/1‑93 (MQ=255)
                                               cTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATT‑TCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGcc                                         <  1:3834270/93‑1 (MQ=255)
                                                tGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATT‑TCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGccc                                        >  1:3965555/1‑93 (MQ=255)
                                                tGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATT‑TCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGccc                                        <  1:2902395/93‑1 (MQ=255)
                                                tGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATT‑TCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGccc                                        <  2:2324156/93‑1 (MQ=255)
                                                   gTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATT‑TCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCa                                     >  2:6220555/1‑93 (MQ=255)
                                                    tCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATT‑TCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCaa                                    >  2:751692/1‑93 (MQ=255)
                                                    tCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATT‑TCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCaa                                    >  1:3861238/1‑93 (MQ=255)
                                                      acaTATTCAAAGCCGTTAAGGATCAGCAGCGGCATT‑TCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCaaaa                                  >  1:2611921/1‑93 (MQ=255)
                                                      acaTATTCAAAGCCGTTAAGGATCAGCAGCGGCATT‑TCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCaaaa                                  >  1:1891425/1‑93 (MQ=255)
                                                      acaTATTCAAAGCCGTTAAGGATCAGCAGCGGCATT‑TCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCaaaa                                  >  2:4441393/1‑93 (MQ=255)
                                                       caTATTCAAAGCCGTTAAGGATCAGCAGCGGCATT‑TCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAAt                                 <  2:6045991/93‑1 (MQ=255)
                                                       caTATTCAAAGCCGTTAAGGATCAGCAGCGGCATT‑TCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAAt                                 >  1:796444/1‑93 (MQ=255)
                                                         tatTGAACGCCGTTAGGGATCAGCAGCGGCATT‑TCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATcc                               <  1:5764661/93‑1 (MQ=255)
                                                         tatTCAAAGCCGTTAAGGATCAGCAGCGGCATT‑TCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATcc                               >  1:1672816/1‑93 (MQ=255)
                                                         tatTCAAAGCCGTTAAGGATCAGCAGCGGCATT‑TCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATcc                               >  2:1035931/1‑93 (MQ=255)
                                                            tCAAAGCCGTTAAGGATCAGCAGCGGCATT‑TCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGa                            >  2:3264537/1‑93 (MQ=255)
                                                               aaGCCGTTAAGGATCAGCAGCGGCATT‑TCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATgg                         <  1:1376276/93‑1 (MQ=255)
                                                               aaGCCGTTAAGGATCAGCAGCGGCATT‑TCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATgg                         <  1:5512820/93‑1 (MQ=255)
                                                               aaGCCGTTAAGGATCAGCAGCGGCATT‑TCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATgg                         <  2:2283885/93‑1 (MQ=255)
                                                               aaGCCGTTAAGGATCAGCAGCGGCATT‑TCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATgg                         >  2:2603656/1‑93 (MQ=255)
                                                                aGCCGTTAAGGATCAGCAGCGGCATT‑TCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGa                        >  2:4375794/1‑93 (MQ=255)
                                                                aGCCGTTAAGGATCAGCAGCGGCATT‑TCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGa                        <  1:3507562/93‑1 (MQ=255)
                                                                   cGTTAAGGATCAGCAGCGGCATT‑TCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGt                     <  1:4505972/93‑1 (MQ=255)
                                                                    gTTAAGGATCAGCAGCGGCATT‑TCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTc                    <  1:344063/93‑1 (MQ=255)
                                                                    gTTAAGGATCAGCAGCGGCATT‑TCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTc                    <  2:2219406/93‑1 (MQ=255)
                                                                     ttAAGGATCAGCAGCGGCATT‑TCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCg                   >  1:5782192/1‑93 (MQ=255)
                                                                     ttAAGGATCAGCAGCGGCATT‑TCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCg                   <  1:4175740/93‑1 (MQ=255)
                                                                     ttAAGGATCAGCAGCGGCATT‑TCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCg                   >  1:823823/1‑93 (MQ=255)
                                                                     ttAAGGATCAGCAGCGGCATT‑TCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCg                   >  2:380877/1‑93 (MQ=255)
                                                                      tAAGGATCAGCAGCGGCATT‑TCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGa                  <  1:4650796/93‑1 (MQ=255)
                                                                      tAAGGATCAGCAGCGGCATT‑TCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGa                  >  2:1350214/1‑93 (MQ=255)
                                                                       aaGGATCAGCAGCGGCATTGC‑CTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAAATGCCCGCAAAATCCGGATGGAAGTCGaa                 <  2:1432553/93‑1 (MQ=255)
                                                                       aaGGATCAGCAGCGGCATT‑TCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGaa                 <  1:5414042/93‑1 (MQ=255)
                                                                       aaGGATCAGCAGCGGCATT‑TCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGaa                 <  2:3671952/93‑1 (MQ=255)
                                                                       aaGGATCAGCAGCGGCATT‑TCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGaa                 <  1:3337303/93‑1 (MQ=255)
                                                                        aGGATCAGCAGCGGCATT‑TCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAAc                <  1:2652573/93‑1 (MQ=255)
                                                                         ggATCAGCAGCGGCATT‑TCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACg               >  2:5026647/1‑93 (MQ=255)
                                                                           aTCAGCAGCGGCATT‑TCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCa             >  2:436127/1‑93 (MQ=255)
                                                                           aTCAGCAGCGGCATT‑TCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCa             <  2:120944/93‑1 (MQ=255)
                                                                           aTCAGCAGCGGCATT‑TCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCa             <  1:4300468/93‑1 (MQ=255)
                                                                           aTCAGCAGCGGCATT‑TCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCa             <  1:2208130/93‑1 (MQ=255)
                                                                              agcagcGGCATT‑TCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAgcg          >  1:6033602/1‑93 (MQ=255)
                                                                              agcagaGGCATT‑TCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTAAACAGAGATGCCCGCAAAATCCGGATGGAAGCCGAACGCAgcg          >  1:4347534/1‑93 (MQ=255)
                                                                                  gcGGCATT‑TCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGt      >  1:5043589/1‑93 (MQ=255)
                                                                                  gcGGCATT‑TCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGt      >  1:1781796/1‑93 (MQ=255)
                                                                                  gcGGCATT‑TCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGt      <  1:2489682/93‑1 (MQ=255)
                                                                                  gcGGCATT‑TCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGt      <  1:610153/93‑1 (MQ=255)
                                                                                  gcGGCATT‑TCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGt      >  2:4102598/1‑93 (MQ=255)
                                                                                    ggCATT‑TCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGttt    >  2:3135974/1‑93 (MQ=255)
                                                                                      cATT‑TCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTcc  <  1:5643511/93‑1 (MQ=255)
                                                                                            |                                                                                       
TTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATT‑T‑CTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCC  >  NC_000913/3115025‑3115202

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTT‑CTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGTAACC  >  NC_000913/3115025‑3115209
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TTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTT‑Cct                                                                                             <  SRR4302533.6006916/94‑3 (MQ=60)
 TGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTT‑Cctg                                                                                            <  SRR4302533.29661/94‑4 (MQ=60)
  GCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACGTATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGC                                                                                            <  SRR4302533.3476425/94‑1 (MQ=60)
  GCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGC                                                                                            >  SRR4302533.4271295/1‑94 (MQ=60)
  GCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGC                                                                                            <  SRR4302533.3180292/94‑1 (MQ=60)
   CTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCG                                                                                           <  SRR4302533.2355044/94‑1 (MQ=60)
   CTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCG                                                                                           >  SRR4302533.966233/1‑94 (MQ=60)
   CTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCG                                                                                           <  SRR4302533.15500/94‑1 (MQ=60)
     GGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGA                                                                                         >  SRR4302533.5060695/1‑94 (MQ=60)
      GTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGAT                                                                                        <  SRR4302533.2433025/94‑1 (MQ=60)
      GTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGAT                                                                                        <  SRR4302533.2094291/94‑1 (MQ=60)
      GTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGAT                                                                                        >  SRR4302533.6310789/1‑94 (MQ=60)
       TATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATC                                                                                       <  SRR4302533.3293577/94‑1 (MQ=60)
       TATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATC                                                                                       <  SRR4302533.2527487/94‑1 (MQ=60)
       TATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATC                                                                                       <  SRR4302533.5459326/94‑1 (MQ=60)
        ATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCG                                                                                      <  SRR4302533.4813707/94‑1 (MQ=60)
         TCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGA                                                                                     <  SRR4302533.6228766/94‑1 (MQ=60)
          CTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAG                                                                                    <  SRR4302533.1734524/94‑1 (MQ=60)
          CTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAG                                                                                    <  SRR4302533.786771/94‑1 (MQ=60)
          CTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAG                                                                                    <  SRR4302533.3772329/94‑1 (MQ=60)
          CTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAG                                                                                    <  SRR4302533.4042841/94‑1 (MQ=60)
           TGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGA                                                                                   <  SRR4302533.327340/94‑1 (MQ=60)
            GCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGAT                                                                                  <  SRR4302533.3876663/94‑1 (MQ=60)
             CACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGTTC                                                                                 >  SRR4302533.3873757/1‑94 (MQ=60)
             CACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATC                                                                                 <  SRR4302533.6152982/94‑1 (MQ=60)
             CACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATC                                                                                 <  SRR4302533.1002930/94‑1 (MQ=60)
               CGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGC                                                                               >  SRR4302533.2335552/1‑94 (MQ=60)
                  AGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCAT                                                                            >  SRR4302533.1150019/1‑94 (MQ=60)
                  AGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCAT                                                                            >  SRR4302533.4339810/1‑94 (MQ=60)
                  AGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCAT                                                                            >  SRR4302533.96351/1‑94 (MQ=60)
                   GCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATA                                                                           <  SRR4302533.5568707/94‑1 (MQ=60)
                    CGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAGGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAG                                                                          >  SRR4302533.6103809/1‑94 (MQ=60)
                    CGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAG                                                                          <  SRR4302533.4332227/94‑1 (MQ=60)
                    CGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAG                                                                          >  SRR4302533.904660/1‑94 (MQ=60)
                    CGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAG                                                                          <  SRR4302533.5616744/94‑1 (MQ=60)
                    CGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAG                                                                          <  SRR4302533.2555370/94‑1 (MQ=60)
                    AGGGATTGCATAAGGATCGTTACCGACGTGAGTCTCATATTCACAGCCGTTAAGGATCAGCAGCTTCATTTCCTGCGGATCGAGATCGCCATAG                                                                          <  SRR4302533.6252686/94‑1 (MQ=60)
                     GGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAATGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGC                                                                         >  SRR4302533.3396361/1‑94 (MQ=60)
                     GGGATTGCATAAGGAGCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGC                                                                         <  SRR4302533.1843469/94‑1 (MQ=60)
                        ATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTAC                                                                      >  SRR4302533.3158921/1‑94 (MQ=60)
                         TTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACT                                                                     <  SRR4302533.2970901/94‑1 (MQ=60)
                           TCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTA                                                                   <  SRR4302533.3990394/94‑1 (MQ=60)
                             ATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAA                                                                 <  SRR4302533.5479625/94‑1 (MQ=60)
                             ATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAA                                                                 >  SRR4302533.4120329/1‑94 (MQ=60)
                             ATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAA                                                                 >  SRR4302533.2120410/1‑94 (MQ=60)
                             ATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAA                                                                 >  SRR4302533.5005062/1‑94 (MQ=60)
                             ATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAA                                                                 >  SRR4302533.1526570/1‑94 (MQ=60)
                              TAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAAT                                                                <  SRR4302533.5575639/94‑1 (MQ=60)
                              TAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAAT                                                                >  SRR4302533.4434001/1‑94 (MQ=60)
                              TAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAAT                                                                >  SRR4302533.5085958/1‑94 (MQ=60)
                              TAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAAT                                                                >  SRR4302533.1674282/1‑94 (MQ=60)
                               AAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCTAGATCGCCATAGCTACTTAAATG                                                               >  SRR4302533.5712288/1‑94 (MQ=60)
                               AAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATG                                                               <  SRR4302533.5009321/94‑1 (MQ=60)
                                 GGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCT                                                             <  SRR4302533.6181082/94‑1 (MQ=60)
                                 GGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCT                                                             <  SRR4302533.2221317/94‑1 (MQ=60)
                                 GGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCT                                                             >  SRR4302533.1792117/1‑94 (MQ=60)
                                  GATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTC                                                            >  SRR4302533.3974425/1‑94 (MQ=60)
                                  GATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTC                                                            <  SRR4302533.3780468/94‑1 (MQ=60)
                                   ATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATTCTCA                                                           <  SRR4302533.1659105/94‑1 (MQ=60)
                                   ATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCA                                                           <  SRR4302533.4870602/94‑1 (MQ=60)
                                   ATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCA                                                           <  SRR4302533.1531447/94‑1 (MQ=60)
                                   ATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCA                                                           <  SRR4302533.6144019/94‑1 (MQ=60)
                                    TCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAA                                                          <  SRR4302533.4285924/94‑1 (MQ=60)
                                     CGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAAC                                                         >  SRR4302533.3287195/1‑94 (MQ=60)
                                     CGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAAC                                                         >  SRR4302533.3450606/1‑94 (MQ=60)
                                     CGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAAC                                                         >  SRR4302533.2904977/1‑94 (MQ=60)
                                      GTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACA                                                        <  SRR4302533.4154816/94‑1 (MQ=60)
                                      GTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACA                                                        <  SRR4302533.3641904/94‑1 (MQ=60)
                                       TTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAG                                                       >  SRR4302533.2267125/1‑94 (MQ=60)
                                          CCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGA                                                    >  SRR4302533.5350182/1‑94 (MQ=60)
                                           CCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGAT                                                   <  SRR4302533.2022551/94‑1 (MQ=60)
                                           CCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGAT                                                   >  SRR4302533.2116890/1‑94 (MQ=60)
                                           CCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGAT                                                   >  SRR4302533.5814304/1‑94 (MQ=60)
                                            CACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATG                                                  >  SRR4302533.2956763/1‑94 (MQ=60)
                                            CACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATG                                                  >  SRR4302533.5271207/1‑94 (MQ=60)
                                             CCCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGC                                                 <  SRR4302533.1768649/94‑1 (MQ=60)
                                             ACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGC                                                 >  SRR4302533.5847071/1‑94 (MQ=60)
                                             ACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAAAAGAGATGC                                                 >  SRR4302533.1427534/1‑94 (MQ=60)
                                              CCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCC                                                >  SRR4302533.4326519/1‑94 (MQ=60)
                                               CTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCC                                               >  SRR4302533.3354017/1‑94 (MQ=60)
                                               CTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCC                                               <  SRR4302533.3857701/94‑1 (MQ=60)
                                               CTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCC                                               >  SRR4302533.3989714/1‑94 (MQ=60)
                                                TGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCG                                              <  SRR4302533.97584/94‑1 (MQ=60)
                                                TGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCG                                              <  SRR4302533.2920223/94‑1 (MQ=60)
                                                  AGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCA                                            >  SRR4302533.414276/1‑94 (MQ=60)
                                                   GTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAA                                           >  SRR4302533.3884808/1‑94 (MQ=60)
                                                     CACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAA                                         >  SRR4302533.2627952/1‑94 (MQ=60)
                                                     CACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAA                                         >  SRR4302533.3387707/1‑94 (MQ=60)
                                                     CACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAA                                         >  SRR4302533.1902970/1‑94 (MQ=60)
                                                      ACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAAT                                        >  SRR4302533.801130/1‑94 (MQ=60)
                                                      ACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAAT                                        >  SRR4302533.5072215/1‑94 (MQ=60)
                                                        ATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCC                                      >  SRR4302533.1682927/1‑94 (MQ=60)
                                                        ATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCC                                      >  SRR4302533.919353/1‑94 (MQ=60)
                                                         TATTGAACGCCGTTAGGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCG                                     <  SRR4302533.5799449/94‑1 (MQ=60)
                                                          ATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGG                                    <  SRR4302533.2224200/94‑1 (MQ=60)
                                                           TTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGA                                   >  SRR4302533.4808745/1‑94 (MQ=60)
                                                           TTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGA                                   >  SRR4302533.3834970/1‑94 (MQ=60)
                                                            TCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAACTACTTAAATGGTCAACAGAGATGCCCGCAAAATCCGGAT                                  >  SRR4302533.2952029/1‑94 (MQ=60)
                                                             CAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATG                                 >  SRR4302533.2652978/1‑94 (MQ=60)
                                                             CAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATG                                 >  SRR4302533.1730135/1‑94 (MQ=60)
                                                              AAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGG                                >  SRR4302533.3143139/1‑94 (MQ=60)
                                                              AAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGG                                >  SRR4302533.5755914/1‑94 (MQ=60)
                                                              AAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGG                                <  SRR4302533.614987/94‑1 (MQ=60)
                                                               AAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGA                               <  SRR4302533.5546099/94‑1 (MQ=60)
                                                               AAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGA                               <  SRR4302533.1384557/94‑1 (MQ=60)
                                                                AGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAA                              <  SRR4302533.3918800/94‑1 (MQ=60)
                                                                AGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAA                              >  SRR4302533.1377616/1‑94 (MQ=60)
                                                                AGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAA                              <  SRR4302533.3529192/94‑1 (MQ=60)
                                                                AGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAA                              <  SRR4302533.5414769/94‑1 (MQ=60)
                                                                 GCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAG                             <  SRR4302533.4072210/94‑1 (MQ=60)
                                                                 GCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAG                             >  SRR4302533.347689/1‑94 (MQ=60)
                                                                  CCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGT                            <  SRR4302533.519611/94‑1 (MQ=60)
                                                                  CCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGT                            >  SRR4302533.5841947/1‑94 (MQ=60)
                                                                   CGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTC                           <  SRR4302533.4533266/94‑1 (MQ=60)
                                                                   CGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTC                           >  SRR4302533.82765/1‑94 (MQ=60)
                                                                    GTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCG                          >  SRR4302533.5817113/1‑94 (MQ=60)
                                                                    GTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCG                          >  SRR4302533.4062404/1‑94 (MQ=60)
                                                                    GTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCG                          >  SRR4302533.828657/1‑94 (MQ=60)
                                                                    GTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCG                          <  SRR4302533.346166/94‑1 (MQ=60)
                                                                     TTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGA                         <  SRR4302533.2354937/94‑1 (MQ=60)
                                                                     TTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGA                         <  SRR4302533.4201042/94‑1 (MQ=60)
                                                                      TAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAA                        <  SRR4302533.4678975/94‑1 (MQ=60)
                                                                       AAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAAC                       <  SRR4302533.5446598/94‑1 (MQ=60)
                                                                       AAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAAC                       <  SRR4302533.3357926/94‑1 (MQ=60)
                                                                        AGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACG                      <  SRR4302533.2668869/94‑1 (MQ=60)
                                                                        AGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATACGGATGGAAGTCGAACG                      >  SRR4302533.5631831/1‑94 (MQ=60)
                                                                          GATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCA                    >  SRR4302533.434193/1‑94 (MQ=60)
                                                                           ATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAG                   >  SRR4302533.2489517/1‑94 (MQ=60)
                                                                           ATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAG                   <  SRR4302533.2221750/94‑1 (MQ=60)
                                                                           ATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAG                   <  SRR4302533.4326331/94‑1 (MQ=60)
                                                                           ATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAG                   >  SRR4302533.6050046/1‑94 (MQ=60)
                                                                             CAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCG                 >  SRR4302533.6070165/1‑94 (MQ=60)
                                                                             CAGCAGAGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTAAACAGAGATGCCCGCAAAATCCGGATGGAAGCCGAACGCAGCG                 >  SRR4302533.4373742/1‑94 (MQ=60)
                                                                              AGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGC                >  SRR4302533.156497/1‑94 (MQ=60)
                                                                                CAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTG              <  SRR4302533.2847037/94‑1 (MQ=60)
                                                                                 AGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGT             >  SRR4302533.5841341/1‑94 (MQ=60)
                                                                                 AGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGT             >  SRR4302533.5074112/1‑94 (MQ=60)
                                                                                 AGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGT             >  SRR4302533.1792637/1‑94 (MQ=60)
                                                                                 AGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGT             >  SRR4302533.170414/1‑94 (MQ=60)
                                                                                  GCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTT            <  SRR4302533.2505014/94‑1 (MQ=60)
                                                                                  GCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTT            <  SRR4302533.613741/94‑1 (MQ=60)
                                                                                   CGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTT           >  SRR4302533.4563814/1‑94 (MQ=60)
                                                                                    GGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTC          >  SRR4302533.3388366/1‑94 (MQ=60)
                                                                                     GCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCC         >  SRR4302533.4478562/1‑94 (MQ=60)
                                                                                      CATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCT        <  SRR4302533.5677625/94‑1 (MQ=60)
                                                                                        TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGT      <  SRR4302533.2941139/94‑1 (MQ=60)
                                                                                        TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGT      <  SRR4302533.1271399/94‑1 (MQ=60)
                                                                                        TTC‑CTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGTA     <  SRR4302533.2303844/94‑1 (MQ=60)
                                                                                        TTC‑CTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGTA     <  SRR4302533.3667655/94‑1 (MQ=60)
                                                                                        TTC‑CTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGTA     <  SRR4302533.5302570/94‑1 (MQ=60)
                                                                                        TTC‑CTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGTA     <  SRR4302533.1227365/94‑1 (MQ=60)
                                                                                         TC‑CTGCGGATCGAGATCGCGATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGTAA    <  SRR4302533.833142/94‑1 (MQ=60)
                                                                                         TC‑CTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGTAA    >  SRR4302533.5130650/1‑94 (MQ=60)
                                                                                          C‑CTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGTAAC   >  SRR4302533.1705674/1‑94 (MQ=60)
                                                                                          C‑CTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGTAAC   >  SRR4302533.3747469/1‑94 (MQ=60)
                                                                                            CTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCGGGATGGAAGTCGAACGCAGCGCTGTTTCCTGTAACC  >  SRR4302533.3402520/1‑94 (MQ=60)
                                                                                            |                                                                                             
TTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTT‑CTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGTAACC  >  NC_000913/3115025‑3115209

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑