Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F13 I0 R1 206 15500.2 9478722 98.3% 9317583 135.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 4,905,178 T→G 20.2% F102V (TTC→GTC)  CIW80_RS24815 → coenzyme F390 synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226864,905,1780TG20.2% 151.1 / 12.6 102F102V (TTC→GTC) CIW80_RS24815coenzyme F390 synthetase
Reads supporting (aligned to +/- strand):  ref base T (29/50);  new base G (21/2);  total (50/52)
Fisher's exact test for biased strand distribution p-value = 2.60e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 106 total aligned reads displayed.

CAGTGGCCAATGATGGATAAAGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCC‑TGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGT‑CGTGGGCTTTTTGTTGTTAGCCCGCACGAGCAACA‑GATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGACGGTTTATTTGCCAAAGAGCGGGTGGCGCT  >  NZ_CP022686/4905040‑4905317
                                                                                                                                           |                                                                                                                                             
aaGTGGCCAATGATGGATAAAGCGGTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAATGCGCGACGAACTTCTCGATTGTGCAATGCGATGTGAGCAGAGTCGTGATTTCAAACAC‑TTCGTTGGCAGGtt                                                                                                                                              <  8:819571/139‑1 (MQ=255)
   tGGCCAATGATGGATAAAGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGGTTTCAAACCC‑AGCGTTGGCAGGTTAAg                                                                                                                                           >  3:163563/1‑140 (MQ=255)
   tGGCCAATGATGGATAAAGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGCTTTAAAACCC‑AGCGGTGGCCGGTTCAg                                                                                                                                           >  3:768491/1‑140 (MQ=255)
   tGGCCAATGATGGATAAAGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTAAAACCC‑AGACTTGGGAGGGTCAg                                                                                                                                           >  3:444364/1‑140 (MQ=255)
   tGGCCAATGATGGATAAAGCGTTAAAGAAGACGCACTTCGATGAGATGAATACCGCAGGATTAAAGCGCGACGAACTTCTAGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCA‑AGAGTTGGCAGCTTCaa                                                                                                                                           >  4:1106418/1‑139 (MQ=255)
   tGGCAAATGATGGATAAAGCGTTAATGATGCCGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGAGTTAAAACAC‑TGCGTTTGCAGGGTCAg                                                                                                                                           >  8:455671/1‑140 (MQ=255)
     gCCAATGATGGATAAAGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGGTTTAAAACCC‑TGCGGTGGGAGGTTGAg                                                                                                                                           >  6:125310/1‑138 (MQ=255)
     gCCAATGATGGATAAAGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTGAAAAAC‑AGCGGTTGCAGGttgat                                                                                                                                           >  5:839938/1‑135 (MQ=255)
       cAATGATTGATAAAGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCC‑TGCGTTGGCAGGTTCAgtg                                                                                                                                         <  5:1160900/138‑1 (MQ=255)
       cAATGATGGATAAATCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCC‑TGCGTTGGCAGGTTCAGTGtt                                                                                                                                       <  4:111822/140‑1 (MQ=255)
       cAATGATGGATAAAGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGTTTTCAAACCC‑AGCGTTGGCCGGTTCAgtg                                                                                                                                         >  1:111100/1‑138 (MQ=255)
       cAATGATGGATAAAGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCC‑TGCGTTGGCAGGTTCAgtg                                                                                                                                         <  1:1014940/138‑1 (MQ=255)
          tgatgGATAAAGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCC‑TGCGTTGGCAGGTTCAGTGTTg                                                                                                                                      <  1:2732/138‑1 (MQ=255)
          tgatgGATAAAGAGTTAATGATGACGCACTTCGCTGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCC‑TGCGTTGGCAGGTTCAGTGTTggg                                                                                                                                    <  3:313920/140‑1 (MQ=255)
            atgGATAAAGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCC‑TGCGTTGGCAGGTTCAGTGTTGGGCt                                                                                                                                  <  4:831166/140‑1 (MQ=255)
               gATAAAGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCTGTGAGCAGAGTCGTGTTTTAAAACAC‑GTCGTTGGCAGGTTGCTTGTTggtggttg                                                                                                                               >  8:142207/1‑134 (MQ=255)
                aTAAAGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCC‑TGCGTTGGCAGGTTCAGTGTTGGGCttt                                                                                                                                <  2:1132416/138‑1 (MQ=255)
                 tacaGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGACCTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCC‑TGCGTTGGCAGGTTCAGTGTTGGGCTTtc                                                                                                                               <  2:1153858/135‑1 (MQ=255)
                 tAAAGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCC‑TGCGTTGGCAGGTTCAGTGTTGGGCTTtcat                                                                                                                             <  7:509082/140‑1 (MQ=255)
                    aGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGCTTTCAAACCC‑TGCGTTGGCAGGGTGAGTGTTGGGGTTTGATCAg                                                                                                                          >  4:1209106/1‑140 (MQ=255)
                    aGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCC‑TGCGTTGGCAGGTTCAGTGTTGGGCTTtcatc                                                                                                                            <  6:251831/138‑1 (MQ=255)
                     gCGTTAATGATGCCGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCC‑TGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAgg                                                                                                                         <  8:693566/140‑1 (MQ=255)
                      ggTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCC‑TGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAg                                                                                                                          <  5:51037/137‑1 (MQ=255)
                        ttAATGATGCCGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGGTTTCAAACCC‑AACGGTTGCAGGTTCAGTGGTGGGCTTTCATAAggg                                                                                                                        >  2:1017320/1‑137 (MQ=255)
                        ttAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGCTTTCAAACCC‑AGCGTTGGGAGGTTAAGTGGTGGGCGGTGATCAGGt                                                                                                                        >  6:5328/1‑138 (MQ=255)
                         tAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCC‑TGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACa                                                                                                                     <  3:370667/140‑1 (MQ=255)
                         acatgaggACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCC‑TGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTa                                                                                                                       <  6:3960/136‑1 (MQ=255)
                          aaTGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTAAAACAC‑AGGCTTGGCCTGTTCAGTGTTGGGGGTTGATAAGGTaa                                                                                                                      >  5:884738/1‑137 (MQ=255)
                           atgatgACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCC‑AGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACa                                                                                                                     >  2:125460/1‑138 (MQ=255)
                           atgatgACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTAAAACCC‑AGCGTTGGCACGTTAAGGGTTTGGCTTTGATCAgctcca                                                                                                                     >  1:847694/1‑133 (MQ=255)
                            tgatgaCGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGTGTTAAAACCC‑AGAGGGTGCAGTGTAAGTGTTTGGCGTTCATCAGGTTCaa                                                                                                                    >  5:573280/1‑137 (MQ=255)
                              atgaCGCACTTCGATGAGATGAATACCGCAGGTTTAATGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCC‑TGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCg                                                                                                                  <  6:85297/138‑1 (MQ=255)
                              atgaCGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCC‑TGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCggg                                                                                                                <  4:923616/140‑1 (MQ=255)
                              atgaCGCACTTAGATGAGATGAATACCTCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTTAGCAGAGTCGTGATTTCAAACCC‑TGCGTTGGCATGTTCAGTGTTGGGCTTTCATCAGGTACATCg                                                                                                                  <  2:847541/138‑1 (MQ=255)
                                  cGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTAAAACCC‑TGCCTTGGGAGGGTCAGTTTTTGGGTTTAATGAGGGTCAACggggg                                                                                                              >  5:820113/1‑136 (MQ=255)
                                  cGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGAGTTCAAACCC‑TGCGTTGGCAGGGTAAGTGTTGGGCGTTCATCAGGGACATCGGgtc                                                                                                              >  2:946385/1‑138 (MQ=255)
                                    cACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCC‑TGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGtcgt‑cg                                                                                                         <  4:356311/140‑1 (MQ=255)
                                          gatgagatgaATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAAACC‑AGCGGTGGCAGGGTCAGTGTTGGGGGTTCATCAGGTTCATCGGGTGGT‑gggggg                                                                                                     >  2:228707/1‑138 (MQ=255)
                                             gagatgaATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCC‑TGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGT‑CGTGGGCtttt                                                                                                <  3:1006741/140‑1 (MQ=255)
                                             gagatgaATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGGGATTTCAAACCC‑TGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGT‑CGTGGGCtttt                                                                                                <  8:45490/140‑1 (MQ=255)
                                                        cGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATTCGCAGTGAGCAGAGTCGTGGTTTAAAACCCAGGCGTGGGCGGTGGCCGGGTGGGGGTTGC‑TCACGTACCTCGGGGAGT‑CGGGGGCGTTTTGGTGTTAGcg                                                                                     >  8:321959/1‑139 (MQ=255)
                                                         gCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGGTTTAAAACAC‑AGCGGTGGGAGGGGGTGTGTTGGGGTTTATTAAGGTTACTCGGGTCGT‑GGGGGGGGTTTTtttgttttg                                                                                      >  5:228108/1‑135 (MQ=255)
                                                          cAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCC‑TGCGTTGGCAGGTTCAGTGTTGGGCTTtcatca                                                                                                                           <  1:237517/101‑1 (MQ=255)
                                                          cAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCC‑TGCGTTGGCAGGTTCAGTGTTGGGCTTtcatca                                                                                                                           >  2:237517/1‑101 (MQ=255)
                                                          cAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCC‑GGCGTTGGCACGGTAAGTGGTTGGCGTTAAAAAAGTTCAAAGGGTAGA‑AGTGGGCGTTTTGTTTTTTGcc                                                                                     >  2:725788/1‑138 (MQ=255)
                                                          cAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGAGTTAAAACAC‑TGCGTTGGCAGGGTGTGTGGTGGGGGTGGATCAGGTACATCGGGTCGT‑GGGGGGCGTTTTtttgttttaccc                                                                                   >  4:210935/1‑134 (MQ=255)
                                                            tgTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCC‑TGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGG‑CGTGGGCTTTTTGTTGTTAGCCCg                                                                                   <  5:516932/137‑1 (MQ=255)
                                                            ggTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAAACC‑AGCGTTGGGAGGTTGAGTGTTTGGCTTTGATCAGGTAAATCGGGTCGG‑CGGGGGGGTTTTTTTGTTatgccc                                                                                   >  2:401229/1‑133 (MQ=255)
                                                            ggTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTAAAACCC‑TGCGTTGGCCGGGTAAGTGTTGGGGATTCATCATGTACATCGGGTCGG‑CGGGGGGGttttttttttttcccc                                                                                   >  6:155052/1‑132 (MQ=255)
                                                              tttAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGTGTCGTGATTTCAAACCC‑TGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGT‑CGTTGGCTTTTTGTTGTTAGCCCGCa                                                                                 <  5:195765/138‑1 (MQ=255)
                                                              tttAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCC‑TGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGT‑CGTGGGCTTTTTGTTGTTAGCCCGCa                                                                                 <  1:55794/138‑1 (MQ=255)
                                                              tttAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCC‑TGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGT‑CGTGGGCTTTTTGTTGTTAGCCCGCACg                                                                               <  3:1208177/140‑1 (MQ=255)
                                                              tttAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCC‑TGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGT‑CGTGGGCTTTTTGTTGTTAGCCCGCACg                                                                               <  3:566253/140‑1 (MQ=255)
                                                              tttAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCC‑TGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGGCGT‑CGTGGGCTTTTTGTTGTTAGCCCGCa                                                                                 <  5:836113/138‑1 (MQ=255)
                                                              tttAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCC‑TGCGTTGGCAGGGTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGT‑CGTGGGCTTTTTGTTGTTAGCCCGCa                                                                                 <  5:331659/138‑1 (MQ=255)
                                                              tttAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGGGATTTCAAACCC‑TGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGT‑CGTGGGCTTTTTGTTGTTAGCCCGCACg                                                                               <  7:595518/140‑1 (MQ=255)
                                                              tttAAAGCGCGACGAACTTCTCGATTGTGCAAAGCTCAGTGAGCAGAGTCGTGATTTCAAACCC‑TGCGTTGGCATGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGT‑CGTGGGCTTTTTGTTGTTAGCCAGCACg                                                                               <  3:94528/140‑1 (MQ=255)
                                                              gttACAGCGCGCCGCACTTCTCGCTTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCC‑TGCGTTGGCAGGTTCAGTGGTGGGCTTTCATCAGGTACATCGGGTCGT‑CGTTGGCTTTTTGTTGTTAGCCCGCACg                                                                               <  4:624896/139‑1 (MQ=255)
                                                                 aaaGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCC‑TGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGT‑CGTGGGCTTTTTGTTGTTAGCCCGCACGAGc                                                                            <  3:956512/140‑1 (MQ=255)
                                                                   aGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCC‑TGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGT‑CGTGGGCTTTTTGTTGTTAGCCCGCACGAGc                                                                            <  5:483847/138‑1 (MQ=255)
                                                                   aGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCC‑TGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGT‑CGTGGGCTTTTTGTTGTTAGCCCGCACGAGCaa                                                                          <  4:778754/140‑1 (MQ=255)
                                                                   aGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCC‑TGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGT‑CGTGGGCTTTTTGTTGTTAGCCCGCACGAGCaa                                                                          <  3:256643/140‑1 (MQ=255)
                                                                     cgcgACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGGTTTAAAACCC‑TGCGGTGGCAGGGTGAGTGTTGGGCTTTCATCAGGTGAATCGGGTCGT‑CGGGGGGGTTTTTTTTTTTGCCCCCCCCAGCAAcc                                                                        >  8:769490/1‑139 (MQ=255)
                                                                       cgacgaACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTGAAACCC‑GGCGGTGGCAGGGTAAGTGTTGGGCGTTCATCAAGTACCTAGGGTCGT‑CGTGGGCTTTTTGTTGTTTGCCCGCACGCGCAACA‑Ga                                                                     >  4:919408/1‑140 (MQ=255)
                                                                       cgacgaACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCC‑AGCGGTGGGAGGGTCAGTGGTGGGCGTTAATAAGGTACATAGGGTAGT‑CGTGGGATTTTTTTTTTTAGCCCGCCCCCGCAACaaa                                                                      >  3:986893/1‑138 (MQ=255)
                                                                       cgacgaACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTAAAACCC‑AGCGTTGGCAGGTTCAGTGTTGGGCTTTAATAAGGTACATCGGGTCGT‑CGTGGGCGTTTTTTTTTTTTCCCGCACCAGCAACaca                                                                      >  4:956512/1‑138 (MQ=255)
                                                                       cgacgaACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGAGTTAAAAACC‑AGCGGTGGCAGGGTTAGTGTAGGGCGTTAAAAAGGTGCATAGGGTCGTAGGTGGGGTTTTT‑TTTTTATACCCCACGAGCcacaca                                                                      >  8:1103990/1‑138 (MQ=255)
                                                                         acgaACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGTTTTCAAACCC‑AGCGTTGGGAGGGTGAGTGTTGGGGTTTCATAAGGTTCATCGGGTCGT‑CGTGGGGTTTTTTTTTTTTGCCCGCACGAGCCACA‑Ga                                                                     >  2:643322/1‑138 (MQ=255)
                                                                                         tgCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCC‑TGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGT‑CGTGGGCTTTTTGTTGTTAGCCCGCACGAGCAACA‑GATGTGGGCCGCCGgtgt                                                     <  5:194635/138‑1 (MQ=255)
                                                                                         tgCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCC‑TGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGT‑CGTGGGCTTTTTGTTGTTAGCCCGCACGAGCAACA‑GATGTGGGCCGCCGgtgt                                                     <  2:251321/138‑1 (MQ=255)
                                                                                         ggCAATGCGCAGGTAGCAGAGGCGTGATTTCAAACCC‑TGCGTTGGCAGGTTAAGTGTTTGGCTTTCATCATGTACATCTGGTAGT‑CGGGTGCTTTTTGTTGTTAGCCCTCAAGAGCAACA‑GATGTGTGCCGCCGgggt                                                     <  5:385231/137‑1 (MQ=255)
                                                                                           cAATGCGCAGTGAGCAGAGTCGTGAGTTCAAACCC‑AGCGGTGGGAGGGGAAGTGGTGGGGTTTAATCAGGTAGAGAGGGTGGT‑GGGGGGGTTTTTTTTGTTTTGCCGCGAGAGCCACA‑GATTGGGGGCGCCCGggtgg                                                   >  2:597485/1‑137 (MQ=255)
                                                                                               gcgcAGTGAGCAGAGTCGTGATTTCAAACCC‑TGCGTTGGCAGGTTCAGTGTTGTGCTTTCATCAGGTACATCGTGTCGT‑CGTGGGCTTTTTGTTGTTAGCCCGCAAGATCAACA‑GATGTGGGCCGCCGGTGTGCTGGc                                               <  6:481676/138‑1 (MQ=255)
                                                                                               gcgcAGTGAGCAGAGTAGGGATTTCAAACCC‑TGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGT‑CGTGGGCTTTTTGTTGTTAGCCCGCACGAGCAACA‑GATGTGGGCCGCCGGTGTGCTGGc                                               <  6:817251/138‑1 (MQ=255)
                                                                                                cgcAGTGAGCAGAGTCGTGATTTGAAACCC‑AGCGTTGGCAGGGTCAGTGTTGGGGTTTCCTCAGGGACATAGGGTCGT‑CGGGGGGGTTTTTTTGTTAGGACCCACCGCCCACA‑AAGGTGGGCCGCCGGTgtggtgtgc                                              >  2:502510/1‑135 (MQ=255)
                                                                                                            agTCGTGATTTAAAAACC‑GGCGGTGGCAGGGTCATTGTTGGGCTTTCATCAGGTACATCGGGTCGG‑GGTGGGGGTTTTTTTTTTTTACCCCCCCCGGAACACGAT‑TGGGCCGCCGCTGTTCGGGCAAAAGTTTTGgcccc                                >  4:892222/1‑138 (MQ=255)
                                                                                                             gTCCTGATTTCAAACCC‑TGCGTTGGCAGCGTCAGGGGTGGGCGTTCATCCGGTACACCGGGTCGT‑CGTGGGCGTTTTGTTGTTAGCCCGAACGAGCAACA‑GATGCGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGAc                               <  8:545893/140‑1 (MQ=255)
                                                                                                                    tttCAAACCC‑TGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGT‑CGTGGGCTTTTTGTTGTTAGCCCGCACGAGCAACA‑GATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGACGGTTTAt                        <  7:409675/140‑1 (MQ=255)
                                                                                                                          aCCC‑TGCGTTGGCAGGTTCAGTGTTGGGCTTTGATCAGGTACATCGGGTCGT‑CGTTGGCTTTTTGTTGTTAGCCCGCACGAGCAACA‑GATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGACGGTTTATTTGc                    <  2:401804/138‑1 (MQ=255)
                                                                                                                          aCCC‑TGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGT‑CGTGGGCTTTTTGTTGTTAGCCCGCACGAGCCACA‑GTTGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGACGGTTTATTTGc                    >  6:579040/1‑138 (MQ=255)
                                                                                                                          aCCC‑TGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGT‑CGTGGGCTTTTTGTTGTTAGCCCGCACGAGCAACA‑GATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGACGGTTTATTTGc                    >  2:283269/1‑138 (MQ=255)
                                                                                                                           ccc‑TGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGT‑CGTGGGCGTTTTGTTGTTAGCCCGCACGTGCCCCA‑GATGGGGGGCGCCGGTGTGCTGGCAAAAGTGTTGGCCGACGGGTTATTTGCCaa                 >  3:314764/1‑140 (MQ=255)
                                                                                                                            cc‑TGCGTTTGCAGGTTCAGTGTTGGTCTTTCATCAGGTACATCTGGTCGT‑CGTGGGCTTTTTGTGGTTAGCCCGCACGAGCAACA‑GATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGACGGTTTATTTGCCa                  <  5:156522/138‑1 (MQ=255)
                                                                                                                            cc‑TGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGCCGT‑CGTGGGCTTTTTGTTGTTAGCCCGCACGAGCAACA‑GCTGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGACGGTTTATTTGCCa                  >  6:999726/1‑138 (MQ=255)
                                                                                                                               tGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGT‑CGTGGGGTTTTTGTTGTTTGACCCCCCGCGCCACA‑GATTGGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGCCGGTTTATTTGGCaaaac              >  7:51544/1‑138 (MQ=255)
                                                                                                                               tGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGT‑CGTGGGCTTTTTGTTGTTAGCACGCACGAGCAACA‑GAAGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGACGGGTTATTTGCCAAaga              >  8:47372/1‑140 (MQ=255)
                                                                                                                               tGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGT‑CGTGGGCTGTTTGTTGTTTGCCCGCACGAGCAACA‑GCTGTTGGCCGCCGGGGGGCTGGCAAAACTGTTGCACGCCGGTTTATTTGCCAAagc              >  4:51134/1‑139 (MQ=255)
                                                                                                                                 cGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGT‑CGTGGGGTTTTTGTTGTTAGCCCGCACGAGCAACA‑GATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGGCCGCCGGGTTATTTGCCAAAGAGc            >  3:962532/1‑140 (MQ=255)
                                                                                                                                  gTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGT‑CGGGGGCTTTTTGTTTTTAGCCCGCACGAGCAACA‑TATGTGGGGCGCCGGTTGGCTGGCAAAATTGTTGCCCGACGGTTTATTTGCCAAagag             >  1:673484/1‑138 (MQ=255)
                                                                                                                                   ttGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGT‑CGTGGGGTTTTTGTTGTTAGCCCGCACGAGCAACA‑GATTTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGACGGTTTATTTGCCAAAGAGc            >  1:255945/1‑138 (MQ=255)
                                                                                                                                    tGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGGCGT‑CGTGGGCTTTTTGTTGTTAGCCCGCACGAGCAACA‑GATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGACGGTTTATTTGCCAAAGAGCggg         <  3:982151/140‑1 (MQ=255)
                                                                                                                                     ggCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGT‑CGTGGGCTTTTTGTTGTTAGCCCGCACGAGCAACA‑GCGGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGCCGGGTTATTTGCGAAAGAGCgg          >  6:505785/1‑138 (MQ=255)
                                                                                                                                       cAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGT‑CGTGGGCTTTTTGTTGTTAGCCCGCACGAGCAACA‑GATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGACGGTTTATTTGCCAAAGAGCGGGt        <  5:737332/138‑1 (MQ=255)
                                                                                                                                       cAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGT‑CGTGGGCATTTTGTTGTTAGCCCGCACCAGCAACA‑GATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGCCCGGTGATTTGCCAAAGCGCGGGttg      >  8:1057060/1‑138 (MQ=255)
                                                                                                                                         ggTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGT‑GGTGGGGTTTTTGTTGTATGCCCCCACGAGCAACA‑GAATTGGGCCGCCGGTGTTCTGGAAAAAGTGTTGACCCACGGTTTATTTGCCAAATGCCGGGTgg      >  5:890019/1‑138 (MQ=255)
                                                                                                                                         ggTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGT‑CGTGGGCTTTTTGTTGTTAGCCCGCACGAGCAACA‑GATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGACGGTTTATTTGCCAAAGAGCGGGTgg      <  1:825566/138‑1 (MQ=255)
                                                                                                                                         ggTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGT‑CGTGGGCTTTTTGTTGTTAGCCCGCACGAGCAACA‑GATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGACGGTTTATTTGCCAAAGAGCGGGTGgcg    <  7:248285/140‑1 (MQ=255)
                                                                                                                                         ggTGCAGTGGTGGGCTGTCATCAGGTACCTCGGGTCGG‑CGTGGGCTTTTTGTTGTTAGCCCGCACGAGCAACA‑GATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGACGGTTTATTTGCCAAAGAGCGGGTgg      <  6:1117892/138‑1 (MQ=255)
                                                                                                                                           ttCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGT‑CGTGGGCTTTTTGTTGTTAGCCCGCACGAGCAACA‑GATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGACGGTTTATTTGCCAAAGAGCGGGTGGCGCt  >  4:977954/1‑140 (MQ=255)
                                                                                                                                           ttCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGT‑CGTGGGCTTTTTGTTGTTAGCCCGCAAGAGCAACA‑AATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGACGGTTTATTTGCCAAAGAGAGGGTGgcgcg  >  4:1083189/1‑139 (MQ=255)
                                                                                                                                           |                                                                                                                                             
CAGTGGCCAATGATGGATAAAGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCC‑TGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGT‑CGTGGGCTTTTTGTTGTTAGCCCGCACGAGCAACA‑GATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGACGGTTTATTTGCCAAAGAGCGGGTGGCGCT  >  NZ_CP022686/4905040‑4905317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A