Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A13 F35 I0 R1 271 8871.0 10354886 98.4% 10189207 136.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 4,905,178 T→G 11.0% F102V (TTC→GTC)  CIW80_RS24815 → coenzyme F390 synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226864,905,1780TG11.0% 209.6 / 2.4 116F102V (TTC→GTC) CIW80_RS24815coenzyme F390 synthetase
Reads supporting (aligned to +/- strand):  ref base T (47/53);  new base G (14/2);  total (61/55)
Fisher's exact test for biased strand distribution p-value = 2.66e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 124 total aligned reads displayed.

CAGTGGCCAATGATGGATAAAGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTT‑CAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGT‑CG‑T‑GGGC‑TTTTTGTTGTTAGCCCGCACGAGCA‑ACAGATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGACGGTTTATTTGCCAAAGAGCGGGTGGCGCT  >  NZ_CP022686/4905040‑4905317
                                                                                                                                           |                                                                                                                                                
cAGTGTCCAATGATGGATAAAGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTT‑CAAACCCTGCGTTGGCAGGtt                                                                                                                                                 <  5:959367/140‑1 (MQ=255)
cAGTGGCCAATTATGGATAAAGCGATAATGATGACGCACTTCGATGAGATTAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTT‑CAAACCCTGCGTTGGCAGGtt                                                                                                                                                 <  7:1131991/140‑1 (MQ=255)
aaGTGGCCAATGATGGATAAAGCGTTAATGATGAAGCACTTAGATGAGATGAATACCGCATGATTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTAGTGATTT‑CAAACCCTGCGTTGGCAGttt                                                                                                                                                 <  8:1103986/139‑1 (MQ=255)
  gttgCCAATGATGGATAAAGCGTTCATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTTCAATGCGCAGTGAGCAGAGGCGTGATTT‑CAAACCCTGAGTTGGCAGGTTCa                                                                                                                                               <  4:707685/137‑1 (MQ=255)
  gTGGCCAATGATGTATACAGCGTTAATGATGACGCACTTCGATGATATGAATACCGCTGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGATTCGTTATTT‑CTAAGCCTGCGTTGGCAGGTTCa                                                                                                                                               <  5:281188/140‑1 (MQ=255)
   tGGCCAATGATGGATAAAGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGTATT‑CTAACCACGCGTTGGCATGTTCat                                                                                                                                              >  5:1135791/1‑139 (MQ=255)
   tGGCCAATGATGGATAAAGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGCTTT‑AAAAACCAGCGTTGGGCGGTTAAg                                                                                                                                              >  8:423181/1‑140 (MQ=255)
   tGGCCAATGATGGATAAAGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTT‑CAAACCCAGCGGTGGCAGGTTCAg                                                                                                                                              >  3:682357/1‑140 (MQ=255)
   tGGCCAATGATGGATAAAGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATAT‑AAAACCCTTCCTGTGCAGGTTCac                                                                                                                                              >  2:807871/1‑139 (MQ=255)
       cAATGATGGATAAAGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTT‑CAAACCCTGCGTTGGCAGGTTCAGTGtt                                                                                                                                          <  6:697643/140‑1 (MQ=255)
        aaTGATGGATAAAGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTT‑AAAACACAGCGGTGGCCGGTTGAGttttt                                                                                                                                         >  7:213308/1‑139 (MQ=255)
          tgatgGATAAAGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTTAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTT‑CAAACCCTGCGTTGGCAGGTTCAGTGTTggg                                                                                                                                       <  4:41516/140‑1 (MQ=255)
               gATAAAGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGCTTT‑AAAACCCAGCGTTGGCAGGTTGATTTTTGGGCgttt                                                                                                                                  >  1:426774/1‑139 (MQ=255)
               gATAAAGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTT‑CAAACCCTGCGTTGGGAGGGTCAGTGTTGGGCgttt                                                                                                                                  >  4:963046/1‑139 (MQ=255)
               gATAAAGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGAGTT‑CAAACCCTTCGGTGGCAGGTTAAGTGTTtggggttc                                                                                                                                  >  8:907268/1‑135 (MQ=255)
                 tAAAGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTT‑CAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTtcat                                                                                                                                <  5:625876/140‑1 (MQ=255)
                 tAAAGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTT‑CAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTtcat                                                                                                                                <  3:515524/140‑1 (MQ=255)
                 tAAAGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGAAGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTT‑CAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTtcat                                                                                                                                <  6:1155451/140‑1 (MQ=255)
                   aaGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTT‑CAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTtcatca                                                                                                                              <  4:1316699/140‑1 (MQ=255)
                    aGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTT‑CAAACCCTGCGTTTGCAGGGTAACTGTTGGGCGTtaatgat                                                                                                                             >  6:631826/1‑137 (MQ=255)
                    aGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTT‑AAAACCCTGGGTTGGGAGGTTCAGTGTTGGGGTTTAATCAg                                                                                                                             >  6:210842/1‑140 (MQ=255)
                    aGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTT‑AAAACCCAGCGTTTGCACGTTCAGTGTTGGGCGTTAATCAg                                                                                                                             >  6:893218/1‑140 (MQ=255)
                     gCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGGCGTGATTT‑CAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAgg                                                                                                                            <  5:825983/140‑1 (MQ=255)
                      cGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTT‑CAAACCCGGCGTTGGCACGGTCAGGGTTGTGCTTTAATAAggg                                                                                                                           >  2:1046621/1‑139 (MQ=255)
                      cGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTT‑CAAACCCAGCGGTGGCAGGTTGAGTGTGTGGCTTTCATCAGGt                                                                                                                           >  4:50408/1‑140 (MQ=255)
                       gTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTT‑CAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTa                                                                                                                          <  7:282537/140‑1 (MQ=255)
                         tAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGCCGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGAATT‑AAAACCCAGCGTTGGCAGGTTAAGATTTGGGCTTTCATCACGTACa                                                                                                                        >  8:320371/1‑140 (MQ=255)
                         tAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTAGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTT‑CAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACa                                                                                                                        <  5:984470/140‑1 (MQ=255)
                         tAATGATGACGCACTTCGATGAGATGAATACCGCAGGGTTAAAGCGCGACGAACTTCTCGATGGTGCAATGCGCAGTGAGCAGAGTCGGGTTTTAAAAACCCTCCG‑TGTCAGGGTCACTGTGTGGCGTtcatcaaggacc                                                                                                                        >  2:84817/1‑134 (MQ=255)
                           atgatgACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTT‑CAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATc                                                                                                                      <  4:146548/140‑1 (MQ=255)
                              atgaCGCACTTCGATTAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTTCAATGCGCAGTGAGCAGAGTCGTGATTT‑CAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCggg                                                                                                                   <  3:530960/140‑1 (MQ=255)
                              atgaCGCACTTCGATTAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGAGCAATTCGCAGTGAGCAGAGTCGTGATTT‑CAAACCCTGCGTTGTCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCggg                                                                                                                   <  5:389148/140‑1 (MQ=255)
                              atgaCGCACTTCGATGAGATGAATACGTCAGGTTTTAAGCGAGACTAACTTCTCGATTGTGCAATGCGCAGTGAGAAGAGTCGTTATTT‑CAAACCCTGCGTTGGCAGTTTCAGTGTTTGGCTTTCATCAGGTACATCggg                                                                                                                   <  1:351246/140‑1 (MQ=255)
                              atgaCGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTT‑CAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCggg                                                                                                                   <  4:908245/140‑1 (MQ=255)
                                gaCGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTT‑CAAACCCAGCGTTGGCAAGGTCAGGGTTGGGCTTTCAAAAGGGACAACGGgtg                                                                                                                 >  7:631936/1‑139 (MQ=255)
                                gaCGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTT‑AAAACCCTGCGTTTGCAGGTTCAGTGTTGGGCTTTAATAAGGTACATAGGgtg                                                                                                                 >  8:594782/1‑139 (MQ=255)
                                     aCTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGGTTT‑AAAACCCAGCGGTGGGAGGGTCAGTGTTGGGGGTTGATCAGGTAAATCGGGgcgt‑cgg                                                                                                           >  4:1108089/1‑139 (MQ=255)
                                             gagatgaATACCGCTGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTT‑CAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGT‑CG‑T‑GGGC‑tttt                                                                                                <  4:998435/140‑1 (MQ=255)
                                             gagatgaATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTT‑CAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCTGGTACATCGGGTCGT‑CG‑T‑GGGC‑tttt                                                                                                <  3:874155/140‑1 (MQ=255)
                                             gagatgaATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTT‑CAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGT‑CG‑T‑GGGC‑tttt                                                                                                <  6:1013836/140‑1 (MQ=255)
                                               gatgaATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTT‑CAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGT‑CG‑T‑GGGC‑TTTttg                                                                                              <  1:1099258/140‑1 (MQ=255)
                                               gatgaATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTT‑CAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGT‑CG‑T‑GGGC‑TTTttg                                                                                              <  3:817902/140‑1 (MQ=255)
                                                atgaATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGGTTT‑CAAAACCTGCGTTGGGAGGGTCAGTGGTGGGCGGTCAAGAGGTTCAACCGGTCGT‑CG‑T‑GGGG‑gtttttt                                                                                             >  7:280061/1‑138 (MQ=255)
                                                   aaTACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTT‑CAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGGCGT‑CG‑T‑GGGC‑TTTTtgttgt                                                                                          <  8:1030938/140‑1 (MQ=255)
                                                   aaTACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTT‑CAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATAAGGTACATCGGGGCGT‑CG‑T‑TGGC‑GTTTttttgt                                                                                          >  6:423350/1‑140 (MQ=255)
                                                   aaTACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGGCGTGATTT‑CAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGT‑CG‑T‑GGGC‑TTTTtgttgt                                                                                          <  6:812303/140‑1 (MQ=255)
                                                     tACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGCGTCGTGGTTT‑CAAACCCGGCGGGGGCGGGTGGAGGGTTGGGGGTTCATCAGGTTCATCGGGGCGG‑GG‑G‑GGGg‑tttttgtttttt                                                                                        >  4:1074724/1‑139 (MQ=255)
                                                         gCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTT‑CAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGT‑CG‑T‑GGGC‑TTTTTGTTGTTTGcac                                                                                    <  4:8483/140‑3 (MQ=255)
                                                          cAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGGTTT‑GCAAACCGGCGTTGGGAGGTTGAGTGTTGGGGGTTGATCAGGTTCGTAGGGGCGTCCG‑G‑GGGG‑TTTTTttggtttaccg                                                                                    >  4:318648/1‑135 (MQ=255)
                                                          cAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGGTTT‑GAAAAACAGCGGTGGGAGGTTGAGGGGTGGGGGTTGATCAGGGACAACGGGGGGT‑CG‑G‑GGGG‑Gttttttttttttgacc                                                                                   >  1:469632/1‑134 (MQ=255)
                                                          cAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGGTTT‑CAAACCCTGCGTTGGCAGGTTCAGTGTTGGGGTTTCATCAGGTTCATCGGGTCGT‑CG‑G‑GGGC‑GTTTTGTTTTTAGCCCg                                                                                   >  6:870071/1‑140 (MQ=255)
                                                          cAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGGTTT‑AAAACCCAGCGTTGGCAGGGTCAGTGTTGGGCTTTGAAAAGGTAAATAGGGTCGT‑CG‑T‑GGGC‑Tttttttttttttcccc                                                                                   >  1:1281750/1‑134 (MQ=255)
                                                          cAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGGTTT‑AAAAACCGGCGGTTGCAGGTTGAGTGTTTGGCGTTGATAAGGTACAAACGGTCGT‑CC‑T‑GGGG‑GTTTTTTTTTTataccc                                                                                   >  3:784041/1‑135 (MQ=255)
                                                          cAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTT‑GAAACCCAGCGGTGGGAGGGTAAGTGTTGGGGGTTAATCAGGTACATCGGGTAGT‑AG‑G‑GGGCGTTTTTTTTTTTGGccc                                                                                    >  5:187150/1‑140 (MQ=255)
                                                          cAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTT‑CAAACCCAGCGTTGGGAGGTTGAGTGTTTGGGGTTGATGAAGTACATAGGGTCGT‑GGTT‑GGGG‑Ttttttttttttcccc                                                                                    >  6:226345/1‑135 (MQ=255)
                                                          cAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATACGCAGTGAGCAGAGTCGTGCTTT‑AAAACACAGCGTTGGGAGGTTCAGTGTTGGGGTTTCATCAGGTACAAAGGGGCGT‑GG‑T‑GGGC‑TTTTTTTTTTTAGcccc                                                                                   >  4:362985/1‑139 (MQ=255)
                                                             gTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGTATT‑CAAACCCAGCGGTGGGAAGTTAAGTTTTGTGCATTGATCAAGTACATCGGGTGGT‑CG‑G‑GGGG‑ATTTTTTTTTTAGGCCGccc                                                                                >  8:205974/1‑138 (MQ=255)
                                                             gTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGGTTT‑CAAACCCAGAGTTGGGAGGTTGAGTGTTGGGGTTTAATAAGTTACATCAGGTCGT‑CG‑G‑GGGG‑TTTTTTTTTTTAGCCCGccc                                                                                >  8:774169/1‑138 (MQ=255)
                                                              tttAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTT‑CAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGT‑CG‑T‑GGGC‑TTTTTGTTGTTAGCCCGCACg                                                                               <  5:544814/140‑1 (MQ=255)
                                                              tttAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTT‑CAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGT‑CG‑T‑GGGC‑TTTTTGTTGTTAGCCCGCACg                                                                               <  7:349254/140‑1 (MQ=255)
                                                                tAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTTAGCAGAGTCGTGATGT‑CAACCCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGT‑CG‑T‑GGGC‑TTTTTGTTGGTAGCCCGCACGAg                                                                             <  8:363103/140‑1 (MQ=255)
                                                                  aaGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGCTTT‑CAAAACCGGCGGTGGCCGGTTGAGTGGTGGGGTTTGAACAGGTGCATCGGGTCGT‑GG‑T‑GGGG‑TTTTTGTTTGTATCCCGCACCAGCa                                                                           >  6:610676/1‑140 (MQ=255)
                                                                   aGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTT‑CAAACCATGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGT‑CG‑T‑GGGC‑TTTTTGTTGTTAGCCCGCACGAGCa‑a                                                                         <  7:856829/140‑1 (MQ=255)
                                                                       cgacgaACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGCTTT‑AAAACCCAGCGGTGGGAGGGTGAGTGTTGGGGTTTCATCAGGGACAACCGGTCGT‑GG‑TGGGGG‑TGTTTTTTGTT‑TCCCGCCCGAGCA‑ACAGa                                                                     >  1:1048642/1‑140 (MQ=255)
                                                                       cgacgaACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTT‑AAAACCCAGCGGTTGGAGGTTGAGTGTTGGGGGTTGATCAGGTTCATGGGGGGGT‑CG‑T‑GGGG‑TTTTTTTTGTTTGCCCCCCCGCGCA‑CCata                                                                     >  2:1307524/1‑138 (MQ=255)
                                                                                 ctcGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTT‑CAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGT‑CG‑T‑GGGC‑TTTTTGTTGTTTTCAATAACTAGCA‑ACAGATGTGGgccga                                                           <  8:927985/140‑2 (MQ=255)
                                                                                   cGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATAT‑CAAACCCTGCGTTGGCAGGGTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGT‑CG‑T‑GGGC‑TTTTTGTTGTTCGCCCGCACGAGCA‑ACAGATGTGGGccgccg                                                         <  2:1288336/140‑1 (MQ=255)
                                                                                     tttGTGCAATGCGCAGTGAGCAGAGTCGGGATTT‑CAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCGTTCATCAGGTACAGCGGGTCGT‑CG‑T‑GGGC‑TTTTTGTTGTTAGCCCGCACGAGCA‑ACAGATGTGGGCCGCCGgt                                                       <  8:848367/139‑1 (MQ=255)
                                                                                         tgCAATGCGCAGTGAGCAGAGTCGTGATTT‑AAAACCCAGCGGTGGCAGGTTAAGTGGTGGGGTGTAATAAGGTTCATCGGGTCGT‑AG‑G‑GGGG‑TTTTTGTTTTTAGCCCGCACGCGCC‑ACAGGAGTGGGCCCCCGGtgtgg                                                   >  4:229789/1‑139 (MQ=255)
                                                                                            aaTGCGCAGTGAGCAGAGTCGTGGTTT‑TAAACCCAGCGTTGGGAGGTTCAGTGTTGGGGTTTGATAAGGTTCATCGGGTCGT‑CG‑G‑GGGG‑GTTTTTTTGTTTTGCCGCACGGGCACACACAT‑TGGGCCGCCGGGGTGCttg                                                >  2:343420/1‑138 (MQ=255)
                                                                                              tGCGCAGTGAGCAGAGTCGTGTTTT‑CAAACCCAGCGTTGGCGCGGTGAGGGTTGGGCTTTTATGAGGTACATCGGGTGGG‑CG‑G‑GGGG‑GTTTTTTTGTTTGCCCGCACGAGCA‑ACAGATGTTGGCCCCCGGTGTGCTGGcc                                              >  4:467797/1‑139 (MQ=255)
                                                                                              tGCGCAGTGAGCAGAGTCGTGGTTT‑GAAACCCTGCGTTTGGAGGTTTAGTGTTGGGGGTTGATAAGGGTAATCGGGGGGG‑CG‑G‑GGGC‑TGTTTGTTTTTTTCACGCACCAGGC‑CAAGATATGGGGCCCCGGggtggggggc                                              >  8:49756/1‑134 (MQ=255)
                                                                                               gcgcAGTGAGCAGAGTCGTGATTT‑CAAACCATGCGTTGGCAGGTTCAGTGTGTGGCTTTCATCAGGTACATCGGGTCGT‑CG‑T‑GGGC‑TTTTTGTTGTTAGCCCGCACGAGCA‑ACAGATGTGGGCCGCCGGTGTGCTGGCaa                                             <  2:344170/140‑1 (MQ=255)
                                                                                                 gcAGTGAGCAGAGTCGTGATTT‑AAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGT‑CG‑T‑GGGC‑TTGTTGTTGTTAGCCCGCACGAGCA‑ACTGATGTGGGCCGCCGGTGTGCTGGCaaaa                                           <  6:340392/140‑1 (MQ=255)
                                                                                                   aGTGAGCAGAGTCGTGATTT‑CAAACCCTGCGTTGGCAGGTTGAGTGTTGGGCTTTGATCAGGTGCATAGGGTCGT‑CG‑G‑GGGC‑TTTTTTTTGTTTTGCCGGACGCGCA‑AAAGATGTGGGCCGGCGGGGTGCGGGCAAAAgt                                         >  4:736475/1‑140 (MQ=255)
                                                                                                     tGAGCAGAGTCGTGATTT‑AAAACCCGGCGGGGGGAGGTTCAGTGTTGGGCGTTCATCAGGTAGATCGGGTCGT‑CG‑T‑GGGG‑TTTTTTTTGTTTACCCGCACACCCC‑ACAACTGTTTGCCGCCCGGGTGCAGGCCAAAatgt                                       >  8:604174/1‑140 (MQ=255)
                                                                                                            agTCGTGATTT‑CAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGT‑CG‑T‑GGGC‑TTTTTGTTGTTAGCCCGCACGAGCA‑ACAGATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGa                                <  2:411498/140‑1 (MQ=255)
                                                                                                            agTCGTGATTT‑CAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGT‑CG‑T‑GGGC‑TTTTTGTTGTTAGCCCGCACGAGCA‑ACAGATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGa                                <  7:903400/140‑1 (MQ=255)
                                                                                                             gTCGTGATTT‑CAAAACCTGCGTTGGCTTGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGG‑GG‑T‑GGGC‑TTTTTGTTGATAGCCCGCACGAGCA‑ACAGATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGAc                               <  6:798746/140‑1 (MQ=255)
                                                                                                                    ttt‑CAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGT‑CG‑T‑GGGC‑TTTTTGTTGTTAGCCCGCACGAGCA‑ACAGATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGACGGTTTAt                        <  3:1214876/140‑1 (MQ=255)
                                                                                                                       taaaaCCCTGCGTTGGCAGTTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGT‑CG‑T‑GGGC‑GTTTTTTTGTTATCCCCCACGGGCA‑ACCCATGTGGGCCGCCGGTGTGCTGGCAAAAGTGGTGCCCGGCGGGtttttt                      >  4:176397/3‑140 (MQ=255)
                                                                                                                         aaaCCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGT‑CG‑T‑GGGC‑TTTTTTTTGTTTTCCCGCACGAGCA‑ACAGATGTGGGCCGGCGGGGTGGTGGCAAAAGTGTTGCCCGACGGGGTATTTgg                    >  6:790502/1‑139 (MQ=255)
                                                                                                                            cccTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGT‑CG‑T‑GGTC‑TTTTTGTTGTTAGCCCGCACGAGCA‑ACAGATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGACGGTTTATTTGCCaa                 <  2:209668/140‑1 (MQ=255)
                                                                                                                             ccTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGT‑CG‑T‑GGGG‑TTTTTGTTGTTAGCCCGCCCGAGCA‑ACAGATGTGGGCCGGCGGTGTGCTGGCAAAAGTGTTGCCCGGCGGGTTTTTTGCCaaa                >  5:494386/1‑140 (MQ=255)
                                                                                                                               tGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGT‑CG‑T‑GGGC‑TTTTTGTTGTTAGCCCGCACGAGCA‑ACAGATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGACGGTTTATTTGCCAAaga              <  1:144744/140‑1 (MQ=255)
                                                                                                                               tGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGT‑CG‑T‑GGGC‑GTTTTGTTGTTAGCCCGCACGAGCC‑ACAGATGGGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGCCGGTTTATTTGCCAAaga              >  2:717045/1‑140 (MQ=255)
                                                                                                                               tGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGT‑CG‑T‑GGGC‑GTTTTGTTCTTAGGCCGCACGCGCC‑AAAGATGTGGGCCGCCGGTGTGCTGGCCAAAGTGTTGCCCGACGGTTTATTTGCCAAaga              >  5:511009/1‑140 (MQ=255)
                                                                                                                               tGCGTTGGCAGGTTCAGTGATGTGCATTCATCAGGTACATCGGGTCGT‑CG‑T‑GGGC‑TTTTTGTTGTTAGGCAGCACGCGGA‑AGAGCTGTGGGCCGCCGGTGTGCTGGCAAAAGTCTTGCCCGCGGGGTTTTTTGGCAAaga              >  8:717344/1‑140 (MQ=255)
                                                                                                                               tGCGTTGGCAGGTTCAGTGATGGGCTTTCATCAGGTACATCGGGTCGT‑CG‑T‑GGGC‑TTTTTGTTGTTAGCCCCCACGAGCA‑ACAGATGTGGGCCGACGGTGTGCTGGCAAAAGTGTTGCCCGACCGGTTATTAGCCAAaga              >  6:881976/1‑140 (MQ=255)
                                                                                                                                 cggtGGCAGGTTCAGACTTGGGCTTACATCAGGTACATCGGGTCGT‑CG‑T‑GGGC‑TTTTTGTTTTAATCACGCAAGACCA‑ACAGCTCAGGCCCCCCGGGGTGCTGGCAAAAGTGTTGCCCGACGGTTTATTTGCAAAagaac            >  2:918621/4‑138 (MQ=255)
                                                                                                                                 cGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGT‑CG‑T‑GGGC‑GTTTTGCTGTTAGCCCGCCCGAGCA‑ACAGATGTGGGCCGCCGGTGTGCTGGAAAAAGTGTTGCCCGACCGTTTATTTGCCAAAGAGc            >  7:785701/1‑140 (MQ=255)
                                                                                                                                     ggCAGGGTCAGTGTTGGGCTTTCATCAGGGACCTCGGGGCGG‑CG‑G‑GGGC‑TGTTTGGTGTTAGCCCGCACGAGCA‑ACAGATGTGGGACGCCGGTGTGCTGGCAAAAGTGTTGCCCGACGGTTTATTTGCCAAAGAGCGGGt        <  8:895858/140‑1 (MQ=255)
                                                                                                                                      gCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGT‑CG‑T‑GGGC‑TTTTTTTTGTTAGCCCGCACGAGGA‑ACAGATTTTGGCCGCCGGTGTGCTGTCCAAAGTGTTGCCCGACCGGTTATTTGCCAAATAGCGGCTg       >  1:1339413/1‑140 (MQ=255)
                                                                                                                                       cctgTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGT‑CG‑G‑GGGC‑TTTTTGTTGCTAGCCCGCACGAGCA‑ACAGATGTGGGCCGCCGGTGTGCTTGCAAAAGTGTTGCCCGACGGTTTATTTGCCAAAGAGCGGGTgg      <  7:1168280/137‑1 (MQ=255)
                                                                                                                                       cAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGT‑CG‑T‑GGGC‑TTTTTGTTGTTAGCCCGCACGAGCA‑ACAGATGTGGGCCGCCGGTGTGCTGGCAAAAGTGGTGCCCGACGGTTTATTTGCCCAAGAGAGGGTgg      >  8:251158/1‑140 (MQ=255)
                                                                                                                                       cAGGTTCAGTGTTGGGCTTTCATCACGTTCATCGGGTCGT‑CG‑T‑CGGC‑TTTTTGTTGTTAGCCCGCACGAGCA‑ACAGATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGACGGTTTATTTGCCAAAGAGCGGGTgg      <  1:540367/140‑1 (MQ=255)
                                                                                                                                         ggTTCATTGTTGGGCTTTCATCAGGTACATCGGGTCGT‑CG‑T‑GGGC‑TTTTTGTTGTTAGCCCGCACGAGCA‑ACAGATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGACGGTTTATTTGCCAAAGAGCGGGTGgcg    <  2:426774/140‑1 (MQ=255)
                                                                                                                                           ttCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGT‑CG‑T‑GGGC‑TTTTTTTTGTTAGCCCGCACGAGCA‑ACAGATGTGGGCCGCCGGTGTGCAGGCAAAAGTGTTGCCCGACGGTTTATTTGCCAAAGAGCGGGTGgcgcg  >  4:426607/1‑139 (MQ=255)
                                                                                                                                           ttCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGT‑CG‑T‑GGGC‑TTTTTGTTGTTAGCCCGCACGAGCA‑ACAGATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGCCGGTTTATTTTCCAAAGAGCGGGTGgcgca  >  7:402645/1‑139 (MQ=255)
                                                                                                                                           ttCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGT‑CG‑T‑GGGC‑TTTTTGTTGTTAGCCCGCACGAGCA‑ACAGATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGACGGTTTATTTGCCAAAGAGCGGGTGGCGCt  >  6:703827/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                                
CAGTGGCCAATGATGGATAAAGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTT‑CAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGT‑CG‑T‑GGGC‑TTTTTGTTGTTAGCCCGCACGAGCA‑ACAGATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGACGGTTTATTTGCCAAAGAGCGGGTGGCGCT  >  NZ_CP022686/4905040‑4905317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A