Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F36 I0 R1	229	12610.8	9774876	98.4%	9618477	135.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP022686	4,905,178	T→G	16.0%	F102V (TTC→GTC)	CIW80_RS24815 →	coenzyme F390 synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP022686	4,905,178	0	T	G	16.0%	181.8 / 4.5	107	F102V (TTC→GTC)	CIW80_RS24815	coenzyme F390 synthetase
Reads supporting (aligned to +/- strand): ref base T (36/51); new base G (17/3); total (53/54)
Fisher's exact test for biased strand distribution p-value = 4.37e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 112 total aligned reads displayed.

CAGTGGCCAATGATGGATAAAGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGTC‑G‑TGGGCTTTTTGTTGTTAGCCCGCACGAGCAACAGATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGACGGTTTATTTGCCAAAGAGCGGGTGG  >  NZ_CP022686/4905040‑4905313

cAGTGGCCAATGATGGATAAAGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCTCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCCTGCGTTGGCAGGtt                                                                                                                                          <  7:168515/140‑1 (MQ=255)

cAGTGGCCAATGATGGATAAAGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCTTGATTTCAAACCCTGCGTTGGCAGGtt                                                                                                                                          <  4:1203777/140‑1 (MQ=255)

cAGTGGCCAATGATGGATAAAGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCCTGCGTTGGCAGGtt                                                                                                                                          <  6:804729/140‑1 (MQ=255)

   tGGCCAATGATGGATAAAGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGAGCAGTGAGCAGAGTCGTGATTTCAAACCCAGCATTGGGAGGTTCat                                                                                                                                       >  6:1107821/1‑139 (MQ=255)

       cAATGATGGATAAAGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCCTGCGTTGGCAGGTTCAGTGtt                                                                                                                                   <  7:438213/140‑1 (MQ=255)

        aaTGATGGATAAAGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTGAAACCCAGCGTTGGCAGGTTCAGTGTTg                                                                                                                                  >  8:686348/1‑140 (MQ=255)

          tgatgGATAAAGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCCTGCGTTGGCAGGTTCAGTGTTg                                                                                                                                  <  1:529587/138‑1 (MQ=255)

          tgatgGATAAAGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCCTGCGTTGGCAGGTTCAGTGTTg                                                                                                                                  <  1:535463/138‑1 (MQ=255)

            ttgGATAAAGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCATACGCTGCGTTGGCAGGTTCAGTGTTGGGCt                                                                                                                              <  3:1185194/139‑1 (MQ=255)

             tgGATAAAGCGTTAATGATGATGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTGAAACCCAGCGGTGGGCGGGTAAGTGTTgggggt                                                                                                                             >  3:1047166/1‑137 (MQ=255)

               gATAAAGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACTAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTtc                                                                                                                           <  3:1229324/140‑1 (MQ=255)

               gATAAAGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTtc                                                                                                                           <  5:873902/140‑1 (MQ=255)

               gATAAAGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTtc                                                                                                                           <  3:187174/140‑1 (MQ=255)

               gATAAAGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTAAAACCCTGCGTGTGCAGGTTCGGTGTTgggggttt                                                                                                                           >  5:1038088/1‑135 (MQ=255)

                aTAAAGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGGGTTCAAAACCCGCGTGGGGAGGTGCACTGGTgggggttga                                                                                                                          >  5:331730/1‑134 (MQ=255)

                 tAAAGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTtc                                                                                                                           <  1:1145314/138‑1 (MQ=255)

                 tAAAGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTtc                                                                                                                           <  2:286754/138‑1 (MQ=255)

                  aaaGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTAAAAACCAGCGTTGGGAGGTTAAGTTTTgggggttcg                                                                                                                          >  2:132590/1‑132 (MQ=255)

                    aGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCCTGCGTTGGGAGGTTCAGTGTTGGGGTTtgatgct                                                                                                                      >  6:15968/1‑137 (MQ=255)

                    aGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAAACCTGCGGTGGGAGGTTCAGTGTTGGGCTTTCATCAg                                                                                                                      >  8:788582/1‑140 (MQ=255)

                     gCGTTAATGATGACGCACGTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGTACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCTTGATTTCATAGACTGCGTTGGCAGGTTCAGTGTTGGGCTTtcatcatg                                                                                                                     <  4:269298/140‑3 (MQ=255)

                      cGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGCTTTCAAACCCAGCGTTGGGAGGTTCAGTGTTGGGCTTTGATCAGGt                                                                                                                    >  5:168721/1‑140 (MQ=255)

                         tAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACa                                                                                                                 <  4:650344/140‑1 (MQ=255)

                          aaTGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAACGCGCGACGACATTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGGATTCTAACCCTTCGTTGGCTGGTTCACTGTTGGGCATTGAGCAGGTTCag                                                                                                                >  8:217484/1‑139 (MQ=255)

                          aaTGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACACTGCGTTGGCAGGTTGAGGGTTGGGCGTTCATCCGGTACAt                                                                                                                >  4:1159608/1‑140 (MQ=255)

                          aaTGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTAAAACCCAACGTTGGGACGTTCAGTTTTGGGCATTCCACAAGTACAt                                                                                                                >  3:483777/1‑140 (MQ=255)

                          aaTGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGAATTAAAACACCGCGGTGGGAGGGTAAGGGTTGGGCGATCATAAGCTACaa                                                                                                                >  3:1098371/1‑139 (MQ=255)

                           atgatgACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTAAAAACCAGCGTTGGCAGGGTCAGTGTTTGGGTTTGATCAGGTACa                                                                                                                 >  1:959201/1‑138 (MQ=255)

                           atgatgACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGAGTTCAAACCCAGCGTTGGCAGGTTAAGTGTTGGGGGTTAGTCAGGTAcc                                                                                                                 >  2:703083/1‑137 (MQ=255)

                              atgaCGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGACCTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCGTGCGTTGGCATGTTCAGTGTTGGGCTTTCATCAGGTACATCggg                                                                                                            <  7:217433/140‑1 (MQ=255)

                                gaCGCGCTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGGGTTCAACCCCAGCGGTGGGAGGGTCAGTGGTGGGCTTTGATCAGGTACAACggggg                                                                                                          >  6:23346/1‑138 (MQ=255)

                                gaCGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTAAAACCCGGCGTTGGGAGGTTCAGTGGTGGGGTTTTATCAGGTACATCCGgtg                                                                                                          >  5:1131941/1‑139 (MQ=255)

                                  cGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGGTTTAAAACCCAGCGTTGGCAGGGTGAGTGTTTGGCGTTCATAAGGTACATCGGgtg                                                                                                          >  1:1250156/1‑137 (MQ=255)

                                  cGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTGAAACCCGGCGTTGGGCGGGTCAGTGTTGGGGGTTAATAAGGTACATCGGgtc                                                                                                          >  1:1241478/1‑138 (MQ=255)

                                    cACTTCGATGAGATGAATACCGCCGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTTATTTCAAACCCTGCGTTGGCAGGTTCAGTGTGGGGCTTTCATCAGGTACATCGGGtcgtc‑g                                                                                                     <  8:793620/140‑1 (MQ=255)

                                       ttCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGTC‑G‑Tgg                                                                                                 <  7:414534/140‑1 (MQ=255)

                                       ttCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTATCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTAAAACCCTGCGTTGGGAAGGTCAGTGTTGGGGTTTTCTCAGGTAGATCGGGtggtc‑ggtg                                                                                                  >  4:100979/1‑137 (MQ=255)

                                         cGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGGTTTCAAAACCAGCGGTGGGAGGTTAAGTGTTGGGCTGTGAACAGGTAAAAAGGGTCGTC‑G‑ggggg                                                                                               >  7:620989/1‑139 (MQ=255)

                                             gagatgaATACGGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCCTTCGTTGGGAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGTC‑G‑TGGGCtttt                                                                                           <  5:1066777/140‑1 (MQ=255)

                                             gagatgaATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGTC‑G‑TGGGCtt                                                                                             <  2:1176426/138‑1 (MQ=255)

                                                 tgaATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGGTTTAAAACCCAGCGGTGGGAGGGTGAGTGGTGGGGGTTGATCAGGTTCATCGGGGCGGG‑G‑GGGGGGTTttgtt                                                                                       >  7:833818/1‑140 (MQ=255)

                                                     tACCGCAGGTTTAAAGCGCGACGCACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCCAGCGTGGGCAGGGTGAGGGGTGGGGGTTATTAAGTGTCATCGGGTCGGC‑G‑TGGGGGTtttttttt                                                                                     >  1:600882/1‑136 (MQ=255)

                                                          cAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGTTTTAAAACCCAGCGTTTGGAGGGTCAGTGTTTGGGTTTGATAAGGTACATCGGGGCGTC‑G‑GGGGGGttttttttttttaccc                                                                              >  6:856832/1‑134 (MQ=255)

                                                          cAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGTTTTAAAAAACAGCGTTGGCAGGTTCAGTGTTGGGGTTTCATCAGGGACAACGGGTCGTCGG‑GGGGGTTTTTTTTTTTTGccc                                                                               >  5:8075/1‑140 (MQ=255)

                                                          cAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGGTTTCAAAACCGGCGGGGGGCGGGTGAGTGTGGGGGGTTCATCAGGGACATCGGGGCGTC‑G‑GGGGGGttttttttttttaccc                                                                              >  3:154683/1‑134 (MQ=255)

                                                          cAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTAAAACCCCGCGTTGGCAGGGTGATTGTTGGGCTTTACTCAGGTAAATCGGGTAGCC‑G‑GTGTCGTTTTTTTTTTAACCCg                                                                              >  4:426664/1‑140 (MQ=255)

                                                           aGGTTTAAAGGGCGCCGAACTTCTCGAATTTGCAATGTGCATTGATCAGATTCGTGAATTCAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTGCATCAGGTACATCGGGTCGGC‑G‑TGGGCGTTTTGTTGTTAGCCCGc                                                                             <  8:1144778/140‑1 (MQ=255)

                                                            ggTTTAACGCGCGACGAACTTCTCTATTGTGAAATGAGCAGTGAGCAGAGTCGTGATTTCAAACCCTGCGTTGGCAGTTTCAGTGTTGGGCTTTCATCAGGTACATCGGGGCGTC‑G‑TGGGCTTTTTGTTGTTAGCCCg                                                                              <  1:679002/138‑1 (MQ=255)

                                                            ggTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGTGTTTAAACCCAGCGTTGGCAGGTTGATTGTTGGGGGTTCATCAGGTTCAAAGGGTCGTG‑G‑TGGGGGTTTTTTTTTTTGcccc                                                                              >  1:1038117/1‑137 (MQ=255)

                                                            ggTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGGTTTAAAACCCTGCGGTGGGAGGTTCAGTGTTGGGGGTTGATCAGGGTCATCGGGTCGTG‑G‑GGGGGGtttttttttttgaccc                                                                              >  2:628092/1‑132 (MQ=255)

                                                            ggTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCCAACCCAGCGGTGGGAGGGTCAGGGGTGGGGTTTCATCAGGTTCATGGGGTGGTC‑G‑TGGGgtttttgtttttttcccc                                                                              >  2:504418/1‑132 (MQ=255)

                                                            ggTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGTGTCGTC‑G‑TGGGCTTTTTGTTGTTAGCCCg                                                                              <  1:1085185/138‑1 (MQ=255)

                                                            ggTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGTC‑G‑TGGGCTTTTTGTTGTTAGCCCGCa                                                                            <  7:861588/140‑1 (MQ=255)

                                                            ggTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGTC‑G‑TGGGCTTTTTGTTGTTAGCCCGCa                                                                            <  5:789266/140‑1 (MQ=255)

                                                            ggTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGTC‑G‑TGGGCTTTTTGTTGTTAGCCCGCa                                                                            <  3:1184847/140‑1 (MQ=255)

                                                            ggTTTAAAGCGCGACGAACTTCTCGATAGTGCAATGCGCAGTGAGCAGAGTCGTGGATGTAAACCCGCCGGTGGCCCGTTCAGTGTTGGGCGTTCATCACGGACAACGGGGCCTC‑GAGGGGGTTTTTtttgtttcccc                                                                               >  1:105551/1‑133 (MQ=255)

                                                            ggTTTAAAGCGCGACGAACGTCTCGATGGTGCAATGCGCAGTGAGCAGAGTCGGGATTTCAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGTC‑G‑TGGGCTTTTTGTTGTTAGCCCGCa                                                                            <  5:268233/140‑1 (MQ=255)

                                                              tttAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGCTTTAAAACCCGGCGGTGGCGGTGTGATAGTTGGGGGTTGATCAGGTAAAACTGGTGGTG‑G‑TTGGGGGTTTGTTTTTTGACCGCACg                                                                          >  4:485783/1‑140 (MQ=255)

                                                              tttAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGTC‑G‑TGGGCTTTTTGTTGTTAGCCCGCa                                                                            <  1:532785/138‑1 (MQ=255)

                                                                 aaaGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGTC‑G‑TGGGCTTTTTGTTGTTAGCCCGCAAGAGc                                                                       <  3:211771/140‑1 (MQ=255)

                                                                  aaGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGTTTTAAAACCCAGCATTGGCAGGTTCAGTTTTGGGGGTTCATGAGGGACATCGGGTCGTG‑G‑TGGGCGTTTTGTTTTTTGCGCGCACGAGCa                                                                      >  4:286458/1‑140 (MQ=255)

                                                                   cgcgcGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCCTGCGTTGGCAGGTGCATTGTTGGGCTTTCATCCGGTACATCGGGGCGTC‑G‑TGGGCTTTTTGTTGTTAGCCAGCACGAGCaa                                                                     <  7:814729/139‑1 (MQ=255)

                                                                   accgcgACCAACTTCTCGATTGTGAAATGCGCAGTGAGCAGATTCGTGATATCAAACCCGGCGTTGGCAGGGGCCGTGTTGTGATTTCATCAGGTACATCGGGGCGTC‑G‑GGGGCTTTTTGTTGTTAGCCCGCACGAGCaa                                                                     <  5:309749/138‑1 (MQ=255)

                                                                   aGCGCGACGAACTTCTCGATTGTGCAATTCGCAGTGAGCAGAGTCGTGATTTCAACCCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGGCGTC‑G‑GGGGCGTTTTGTAGTTAGCCCGCACGAGc                                                                       <  1:760136/138‑1 (MQ=255)

                                                                   aGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGTC‑G‑TGGGCTTTTTGTTGTTAGCCCGCACGAGCaa                                                                     <  5:856832/140‑1 (MQ=255)

                                                                   aGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGTC‑G‑TGGGCTTTTTGTTGTTAGCCCGCACGAGCaa                                                                     <  6:131179/140‑1 (MQ=255)

                                                                   aGCGCGACGAACTTCTCGATTGTGCAATGCGCAGAGAGCAGAGTCGTGATTTCAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGTC‑G‑TGGGCTTTTTGTTGTTAGCCCGCACGAGCaa                                                                     <  7:1162345/140‑1 (MQ=255)

                                                                    gcgcGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGTC‑G‑TGGGCTTTTTGTTGTTAGCCCGCACGAGCAAc                                                                    <  3:207180/140‑1 (MQ=255)

                                                                       cgacgaACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGTTTTAAAACCCAGCGGTGGCAGGGTGAGTGTTGGGGGTTGATACGGTACATCGGGTCGTA‑G‑GGGGGTGTTTGTTGGTTGCCCGCCCCAGCAACAGa                                                                 >  8:1038510/1‑140 (MQ=255)

                                                                       cgacgaACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGCTTTAAAAACCTGCGGTGGCAGGTTGAGTGTTGGGCGTTTATCAAGTACATACGGTCGTC‑G‑TGTGGTTGTTTTTTTTATACCACACGCGCaaaaaa                                                                 >  3:884271/1‑136 (MQ=255)

                                                                       cgacgaACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCCAGCGTTGGGAGGTTAAGTGTTGGGCTTTCATCAGGTAGATCGGGTGGTG‑G‑TGGGGGTTTTTTTTTTATACCCCCCGAGCcacaca                                                                 >  4:358672/1‑138 (MQ=255)

                                                                        gacgaACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTAAAACCCGGCGTTGGCAGGTTCAGTGTTGGGCGTTCATCAGGTAAATAGGGTCGTC‑G‑TGGGGGTTTTTTTTTTAGACCCCCCCCGCCACAGAt                                                                >  4:822824/1‑140 (MQ=255)

                                                                         acgaACTTCTCGATTGTGCAATGCGCAGTGAGCAAAGTCGTGATTTCAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGTC‑G‑TGGGCAGTTTGTTGTTTGCCCCCACGAGCcacaca                                                                 >  1:1089073/1‑136 (MQ=255)

                                                                              cTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGTC‑G‑TGGTCTTTTTGTTGTTAGCCCGCACGAGCAACAGATGTgg                                                            <  2:263918/138‑1 (MQ=255)

                                                                              cTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCCTGAGTTGGCAGGGTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGTC‑G‑TGGGCTTTTTGTTGTTAGCCCGCACGAGCAACAGATGTgg                                                            <  1:603876/138‑1 (MQ=255)

                                                                              attatcGTTTGTGCAATGCGCAGTGAGCAGAGTCGTGATATCAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGTGTCGTC‑G‑TGGGCTTTTTGTTGTTAGCCCGCACGAGCAACAGATGTGGgc                                                          <  5:44064/136‑1 (MQ=255)

                                                                                   cGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCCTGCGTTGGCAGGTTCAGTGGTGGGCTTTCATCAGGTACATCGGGTCGTC‑G‑TTGGCTTTTTGTTGTTAGCCCGCACGAGCAACAGATGTGGgccgc                                                       <  1:1205693/138‑1 (MQ=255)

                                                                                        gtgCATTGCGCTGTGAGCTGAGTCGTGATTTCAACCCCTGCGTTGGCTGGTTCAGTGGTGGGCTTTCATCAGGTTCATCGGGTCGTC‑G‑TGGGCTTTTTGTTGTTAGGCCGCAGGAGCAACAGATGTGGGCCGCCGGtgtg                                                <  5:118861/140‑1 (MQ=255)

                                                                                         tgCAATGCGCAGTGAGCAGAGTCGTGATTTAAAACCCTGCGTTGGCCGGGTAATTGTTGGGCTTTAATCAGGGACAACGGGGCGTC‑G‑GGGGGATTTTTTTGTTTGCCCGCACGCGCACCAACTGTGGGCAGCCGGtgtgg                                               >  8:163536/1‑139 (MQ=255)

                                                                                          gCAATGCGCAGTGAGCAGAGGCGTGATTTCAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGGC‑G‑TGGGCTTTTTGTTGTTAGCCCGCACGAGCAACAGATGTGGGCCGCCGGTGTGCt                                              <  4:1088446/140‑1 (MQ=255)

                                                                                              tGCGCAGTGAGCAGAGTCGTGCTTTCAAACCCGGGGGTGGGAGGTTAATGGTTGGGGTTTCCTCAGGTACATAGGGGCGGA‑G‑TGGGGTGTTTTTTTTTTGCCCCCACCAGGAACAGTTGTGGGGCGCCGGTTTTCTgggc                                          >  4:971962/1‑138 (MQ=255)

                                                                                                  cAGTGAGCAGAGTCGTGGTTTAAAACCCAGCGGTGGCAGGTTCAGTGTTGGGGGTTCATCAGGGACAAAGGGTGGGA‑G‑TGGGGATTTTTTTGTTAGCCCCCACGCACAACAGATGTTGGGCGCCCGGGtggtgggcaa                                        >  2:347893/1‑134 (MQ=255)

                                                                                                                    tttCACACCCTGCGTTGGCAGGTTCAGTCTTGGGCGTTCATCAGGTACATCGGGGCGTC‑G‑TGGGCTTTTTGTTGTTAGCCCGCACGAGCAACAGATGTGGGCCGCCGGTGTGCTGGCAAAAGGGTTGCCCGACGGTTTAt                    <  3:985033/140‑1 (MQ=255)

                                                                                                                    tttCAAACCCGGCGTGGGCAGGTTCAGTGGTGGGCTTTCATCAGGTACATCGGGTCGGC‑G‑TGGGCGTTTTGTTGTTAGCCCGCACGAGCAACAGATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGACGGTTTAt                    <  6:988870/140‑1 (MQ=255)

                                                                                                                     ttCAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGGCGGC‑G‑TGGGCTTTTTGTTGTTAGCCCGCACGAGCAACAGATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGACGGTTTAtt                   <  4:41683/140‑1 (MQ=255)

                                                                                                                        aaaCCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGTC‑G‑TGGGGTTTTTGTTGTTAGCCCGCCCGAGCAACAGATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGACGGTTTATTTGc                >  3:1209098/1‑140 (MQ=255)

                                                                                                                        aaaCCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGTC‑G‑TGGGCTTTTTGTTGTTAGCCCGCACGAGCAACAGATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGACGGTTTATTTGc                <  7:686348/140‑1 (MQ=255)

                                                                                                                         aaCCCTGCGTTGGCAGGTGCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGTC‑G‑TGGGCTTTTTGTTGTTAGCCCGCACGAGCAACAGATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGACGGTTTATTTGcc               <  4:738651/140‑1 (MQ=255)

                                                                                                                          aCCCTGCGTTGGCATGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGTC‑G‑TGTGCTTTTTGTTGTTCGCCCGCACGAGCAACAGATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGACGGTTTATTTGCCa              <  5:277752/140‑1 (MQ=255)

                                                                                                                          aCCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGTC‑G‑TGGGCTTTTTGTTGTTAGCCCGCACGAGCAACAGATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGACGGTTTAttttc                >  2:1142553/1‑136 (MQ=255)

                                                                                                                           cccTGCGTTGGCAGGGTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGTC‑G‑TGGGCTTTTTGTTGTTAGCCCGCACGAGCAACAGATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGACGGTTTATTTGcc               <  1:232546/138‑1 (MQ=255)

                                                                                                                              tGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGTC‑G‑TGGGCTTTTTGTTGTTAGCCCGCACGAGCAACAGATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGACGGTTTATTTGCCAAaga          >  5:551603/1‑140 (MQ=255)

                                                                                                                                cGTTGTCAGGTTCCGTGTTGGGCTATCATCCGGTACATCGGGTCGGC‑G‑TGGGCTCTTTGTTGTTAGCCCGCACGAGCAACAGCTGCGGGCCGCCGGTGTGCTGGCAAAAGTGCTGCCCGACGGGTTATTTGCCAAaga          <  2:986548/138‑1 (MQ=255)

                                                                                                                                cGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGTC‑G‑TGGGCTTTTTGTTGTTAGCCCGCACGAGCAACAGATGTGGgc                                                          <  8:354318/90‑1 (MQ=255)

                                                                                                                                cGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGTC‑G‑TGGGCTTTTTGTTGTTAGCCCGCACGAGCAACAGATGTGGgc                                                          >  7:354318/1‑90 (MQ=255)

                                                                                                                                  ttGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGTC‑G‑TGGGCTGTTTGTTGTTAGCCCGCACGAGCAACAGATGTGGGCCGGCGGGGGGCTGGCAAAAGTGTTTCCCTCCGGTTTTTTTGCCAAagagg        >  2:1184807/1‑137 (MQ=255)

                                                                                                                                   tGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGTC‑G‑GGGGCTTTTTGTTGGTAGCCCGCACGAGCAACAGATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGACGGTTTATTTGCCAAAGAGCggg     <  6:807301/140‑1 (MQ=255)

                                                                                                                                    ggCAGGTTCAGGGTTGGGCTTTCATCAGGTACTATGGGTCGTC‑G‑GGGGCTTTTTGTTGGTAGCCCGCTCGAGCAACAGATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGACGGTTTATTTGCCAAAGAGCGGGt    <  8:667698/140‑1 (MQ=255)

                                                                                                                                      cAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGTC‑G‑TGGGCTTTTTGTTGTTAGCCCGCACGAGCAACAGATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGACGGTTTATTTGCCAAAGAGCGGGTgg  <  6:776541/140‑1 (MQ=255)

                                                                                                                                      cAGGTGCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGTC‑G‑TGGGCTCTTTGTTGTTAGCCCGCACGAGCAACAGATGGGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGACGGTTTATTTGCCAAAGAGCGGGTgg  <  5:286363/140‑1 (MQ=255)

CAGTGGCCAATGATGGATAAAGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAGGTACATCGGGTCGTC‑G‑TGGGCTTTTTGTTGTTAGCCCGCACGAGCAACAGATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGACGGTTTATTTGCCAAAGAGCGGGTGG  >  NZ_CP022686/4905040‑4905313

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A