Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F2 I225 R1
|
227 |
21.4 |
1179702 |
97.1% |
1145490 |
86.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NZ_CP009273 |
1,300,524 |
Δ1 bp |
coding (504/1005 nt) |
oppF → |
murein tripeptide/oligopeptide ABC transporter ATP‑binding protein OppF |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NZ_CP009273 | 1,300,522 | 0 | G | . | 100.0%
| 63.3
/ NA
| 16 | coding (502/1005 nt) | oppF | murein tripeptide/oligopeptide ABC transporter ATP‑binding protein OppF |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base . (6/10); total (6/10) |
GAAAGTCGGGTTATTGCCTAACCTGATTAACCGCTATCCGCATGAGTTCTCTGGTGGGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGC > NZ_CP009273/1300467‑1300603
|
gAAAGTCGGGTTATTGCCTAACCTGATTAACCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGt < 1:315837/84‑1 (MQ=255)
gAAAGTCGGGTTATTGCCTAACCTGATTAACCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGt > 2:315837/1‑84 (MQ=255)
gAAAGTCGGGTTATTGCCTAACCTGATTAACCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCtt > 1:377964/1‑90 (MQ=255)
ccTAACCTGATTAACCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGc < 1:21959/90‑1 (MQ=255)
tAACCTGATTAACCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTAt < 1:60514/53‑1 (MQ=255)
tAACCTGATTAACCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTAt > 2:60514/1‑53 (MQ=255)
gATTAACCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGAtt < 1:437102/87‑1 (MQ=255)
gATTAACCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGAtt < 1:558617/87‑1 (MQ=255)
gATTAACCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGAtt > 2:437102/1‑87 (MQ=255)
gATTAACCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGAtt > 2:558617/1‑87 (MQ=255)
gATTAACCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATc < 1:18851/90‑1 (MQ=255)
gATTAACCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATc < 2:20641/90‑1 (MQ=255)
aTCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGcc > 1:84150/1‑90 (MQ=255)
aTGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTc < 2:377964/90‑1 (MQ=255)
aTGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTc < 2:519999/90‑1 (MQ=255)
ttCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGgcgc < 1:126419/90‑1 (MQ=255)
|
GAAAGTCGGGTTATTGCCTAACCTGATTAACCGCTATCCGCATGAGTTCTCTGGTGGGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGC > NZ_CP009273/1300467‑1300603
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
GCGTGAAGGCGATGATGCTGAAAGTCGGGTTATTGCCTAACCTGATTAACCGCTATCCGCATGAGTTCTCTGGTGGGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGCTGGACGTGTCAATTCAGGCA > NZ_CP009273/1300448‑1300623
|
gcagcgtcagatgtgtataagagacaGGGTTATTGCCTAACCTGATTAACCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCG < SRR3722109.61238/74‑1 (MQ=60)
CGATGATGCTGAAAGTCGGGTTATTGCCTAACCTGATTAACCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTT > SRR3722109.383319/1‑100 (MQ=60)
GATGCTGAAAGTCGGGTTATTGCCTAACCTGATTAACCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTC < SRR3722109.319993/100‑1 (MQ=60)
CCTAACCTGATTAACCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTG < SRR3722109.22246/100‑1 (MQ=60)
CCTGATTAACCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATG < SRR3722109.443567/100‑1 (MQ=60)
CCTGATTAACCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATG < SRR3722109.567086/100‑1 (MQ=60)
GATTAACCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGC < SRR3722109.19096/100‑1 (MQ=60)
ATTAACCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCC > SRR3722109.85183/1‑100 (MQ=60)
TTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGCTGGACGTGTC < SRR3722109.127976/100‑1 (MQ=60)
GCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGCTGGACGTGTCAATTCAGGCA < SRR3722109.82287/100‑1 (MQ=60)
|
GCGTGAAGGCGATGATGCTGAAAGTCGGGTTATTGCCTAACCTGATTAACCGCTATCCGCATGAGTTCTCTGGTGGGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGCTGGACGTGTCAATTCAGGCA > NZ_CP009273/1300448‑1300623
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
