Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F2 I195 R1
|
222 |
27.1 |
1551108 |
96.0% |
1489063 |
86.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NZ_CP009273 |
1,300,524 |
Δ1 bp |
coding (504/1005 nt) |
oppF → |
murein tripeptide/oligopeptide ABC transporter ATP‑binding protein OppF |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NZ_CP009273 | 1,300,522 | 0 | G | . | 100.0%
| 58.7
/ NA
| 15 | coding (502/1005 nt) | oppF | murein tripeptide/oligopeptide ABC transporter ATP‑binding protein OppF |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base . (7/8); total (7/8) |
GAAGTTCGCGAGCGCGTGAAGGCGATGATGCTGAAAGTCGGGTTATTGCCTAACCTGATTAACCGCTATCCGCATGAGTTCTCTGGTGGGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCG > NZ_CP009273/1300435‑1300602
|
gAAGTTCGCGAGCGCGTGAAGGCGATGATGCTGAAAGTCGGGTTATTGCCTAACCTGATTAACCGCTATCCGCATGAGTTCTCtggtggc < 2:608566/90‑2 (MQ=255)
cgAGCGCGTGAAGGCGATGATGCTGAAAGTCGGGTTATTGCCTAACCTGATTAACCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAg < 2:160594/90‑1 (MQ=255)
gAGCGCGTGAAGGCGATGATGCTGAAAGTCGGGTTATTGCCTAACCTGATTAACCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGc < 2:552461/90‑1 (MQ=255)
atgatgCTGAAAGTCGGGTTATTGCCTAACCTGATTAACCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTgcgc > 1:389650/1‑90 (MQ=255)
gatgCTGAAAGTCGGGTTATTGCCTAACCTGATTAACCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGt < 2:547737/90‑1 (MQ=255)
atgCTGAAAGTCGGGTTATTGCCTAACCTGATTAACCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTg < 2:368455/90‑1 (MQ=255)
tGAAAGTCGGGTTATTGCCTAACCTGATTAACCGCTATCCGCATGAGTTCTCGGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGctct > 1:669107/1‑90 (MQ=255)
gggTTATTGCCTAACCTGATTAACCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTg > 2:396345/1‑90 (MQ=255)
tATTGCCTAACCTGATTAACCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCtt < 1:635648/79‑1 (MQ=255)
tATTGCCTAACCTGATTAACCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCtt > 2:635648/1‑79 (MQ=255)
tGCCTAACCTGATTAACCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGaa < 2:766350/90‑1 (MQ=255)
cTAACCTGATTAACCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCt > 2:141345/1‑90 (MQ=255)
ccTGATTAACCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGAtt < 1:418891/90‑1 (MQ=255)
ccGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGtgat < 1:329918/90‑1 (MQ=255)
cATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGgtgt > 1:751665/1‑90 (MQ=255)
gTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGCGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGgcg > 1:533840/1‑90 (MQ=255)
|
GAAGTTCGCGAGCGCGTGAAGGCGATGATGCTGAAAGTCGGGTTATTGCCTAACCTGATTAACCGCTATCCGCATGAGTTCTCTGGTGGGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCG > NZ_CP009273/1300435‑1300602
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
ACGCCAGGAAGTTCGCGAGCGCGTGAAGGCGATGATGCTGAAAGTCGGGTTATTGCCTAACCTGATTAACCGCTATCCGCATGAGTTCTCTGGTGGGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGCTGGACGTGTCAATT > NZ_CP009273/1300428‑1300617
|
ACGCCAGGAAGTTCGCGAGCGCGTGAAGGCGATGATGCTGAAAGTCGGGTTATTGCCTAACCTGATTAACCGCTATCCGCATGAGTTCTCTGGT‑GGCAGT < SRR3722073.168899/100‑1 (MQ=60)
CGTGAAGGCGATGATGCTGAAAGTCGGGTTATTGCCTAACCTGATTAACCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGC > SRR3722073.397948/1‑100 (MQ=60)
GCGATGATGCTGAAAGTCGGGTTATTGCCTAACCTGATTAACCGCTATCCGCATGAGTTCTCGGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCT > SRR3722073.682801/1‑100 (MQ=60)
GAAAGTCGGGTTATTGCCTAACCTGATTAACCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAAC < SRR3722073.648779/100‑1 (MQ=60)
CCTGATTAACCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATG < SRR3722073.427844/100‑1 (MQ=60)
CCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGT < SRR3722073.336927/100‑1 (MQ=60)
CCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGT > SRR3722073.766885/1‑100 (MQ=60)
ATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGCGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCG > SRR3722073.544982/1‑100 (MQ=60)
CTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGCTGGACGTGTCAATT > SRR3722073.250577/1‑100 (MQ=60)
|
ACGCCAGGAAGTTCGCGAGCGCGTGAAGGCGATGATGCTGAAAGTCGGGTTATTGCCTAACCTGATTAACCGCTATCCGCATGAGTTCTCTGGTGGGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGCTGGACGTGTCAATT > NZ_CP009273/1300428‑1300617
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
