Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,195,449 A→T 100% E564D (GAA→GAT)  yehI → DUF4132 domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,195,4490AT97.7% 877.4 / 6.9 256E564D (GAA→GAT) yehIDUF4132 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (6/0);  new base T (127/122);  total (133/123)
Fisher's exact test for biased strand distribution p-value = 3.03e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 256 total aligned reads displayed.

CTGCACGCAATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGAAAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTTTCTCAG  >  CP009273/2195304‑2195595
                                                                                                                                                 |                                                                                                                                                  
ctgcACGCAATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATg                                                                                                                                                >  1:4514282/1‑150 (MQ=255)
  gcACGCAATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGAc                                                                                                                                              >  1:1614627/1‑150 (MQ=255)
   cACGCAATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACa                                                                                                                                             <  1:2606208/150‑1 (MQ=255)
   cACGCAATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACa                                                                                                                                             >  1:2155574/1‑150 (MQ=255)
        aaTTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAactac                                                                                                                                        <  2:309909/150‑1 (MQ=255)
           tCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTca                                                                                                                                     <  2:4077103/150‑1 (MQ=255)
           tCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTca                                                                                                                                     >  2:2171795/1‑150 (MQ=255)
            cACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTcac                                                                                                                                    <  1:584709/150‑1 (MQ=255)
             aCTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTcaca                                                                                                                                   <  1:1246761/150‑1 (MQ=255)
              ctctTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACAt                                                                                                                                  <  1:293149/150‑1 (MQ=255)
               tctTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACAtt                                                                                                                                 <  1:3898913/150‑1 (MQ=255)
                 tttGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTAt                                                                                                                               <  1:2400543/150‑1 (MQ=255)
                   tGTTCAGTGGCATTACTACCAGGGGCTGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACTGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCg                                                                                                                             <  2:1741168/150‑1 (MQ=255)
                     ttCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTc                                                                                                                           <  1:2450772/150‑1 (MQ=255)
                       cAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCg                                                                                                                         <  2:2828575/150‑1 (MQ=255)
                        aGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCgg                                                                                                                        >  1:1250885/1‑150 (MQ=255)
                         gTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGa                                                                                                                       <  2:1794349/150‑1 (MQ=255)
                              aTTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAaga                                                                                                                  >  1:1722608/1‑150 (MQ=255)
                              aTTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAaga                                                                                                                  >  2:110142/1‑150 (MQ=255)
                               ttACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAagag                                                                                                                 <  1:496972/150‑1 (MQ=255)
                               ttACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAagag                                                                                                                 <  1:2815800/150‑1 (MQ=255)
                                tactacCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAgt                                                                                                                >  2:4012799/1‑150 (MQ=255)
                                  ctacCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAgtgt                                                                                                              >  2:2362484/1‑150 (MQ=255)
                                   tacCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTc                                                                                                             >  1:925758/1‑150 (MQ=255)
                                    acCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCt                                                                                                            <  1:1282865/150‑1 (MQ=255)
                                       aGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGa                                                                                                         >  2:3607936/1‑150 (MQ=255)
                                       aGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGa                                                                                                         >  1:3619295/1‑150 (MQ=255)
                                           gCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCt                                                                                                     <  1:2795638/150‑1 (MQ=255)
                                             gggTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGt                                                                                                   >  2:1153036/1‑150 (MQ=255)
                                                ttGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGtatt                                                                                                >  1:307598/1‑150 (MQ=255)
                                                ttGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGtatt                                                                                                >  1:270015/1‑150 (MQ=255)
                                                ttGAAGAGATCCTGAATGGCTGGAAATCCCCGGAAGCACAAATAGCAGAACAGGCGCTCCGCAGCGGACACATAGAAGCGTTAATTAACATAATGGATAATGACAACTACTCACAGTATCGTCCGGACAAGAGTGTCTGGAACCTGtatt                                                                                                >  1:3695062/1‑150 (MQ=255)
                                                 tGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCCGtattt                                                                                               >  2:3064416/1‑150 (MQ=255)
                                                   aaGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGtatttat                                                                                             <  2:321349/150‑1 (MQ=255)
                                                      agaTCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTgg                                                                                          >  2:2554846/1‑150 (MQ=255)
                                                          ccTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGcaca                                                                                      >  1:4139619/1‑150 (MQ=255)
                                                          ccTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGcaca                                                                                      <  2:3390708/150‑1 (MQ=255)
                                                             gAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGtt                                                                                   <  2:1904505/150‑1 (MQ=255)
                                                                tGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGcc                                                                                >  2:2023568/1‑150 (MQ=255)
                                                                tGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGcc                                                                                >  2:945825/1‑150 (MQ=255)
                                                                 ggCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCcg                                                                               <  2:4241346/150‑1 (MQ=255)
                                                                  gCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCcgc                                                                              >  2:2491850/1‑150 (MQ=255)
                                                                  gCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCcgc                                                                              >  1:4366996/1‑150 (MQ=255)
                                                                   cTGGAACTCACCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAATCGTTAATTACCATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCcgcg                                                                             <  2:238539/150‑1 (MQ=255)
                                                                    tGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGt                                                                            >  1:1320677/1‑150 (MQ=255)
                                                                     ggAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTg                                                                           >  1:3045442/1‑150 (MQ=255)
                                                                       aaCTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTgag                                                                         <  2:2455433/150‑1 (MQ=255)
                                                                         cTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGAt                                                                       <  1:1900514/150‑1 (MQ=255)
                                                                         cTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGAt                                                                       <  1:2497017/150‑1 (MQ=255)
                                                                         cTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGACACAGTTGCCGCGTGAGAt                                                                       >  1:3368507/1‑150 (MQ=255)
                                                                         cTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGCGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGAt                                                                       <  2:2970433/150‑1 (MQ=255)
                                                                          tCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATg                                                                      <  2:834240/150‑1 (MQ=255)
                                                                          tCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATg                                                                      >  1:2539085/1‑150 (MQ=255)
                                                                           ccccGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATgg                                                                     >  2:521642/1‑150 (MQ=255)
                                                                           ccccGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATgg                                                                     <  2:2117921/150‑1 (MQ=255)
                                                                            cccGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGc                                                                    >  2:4259409/1‑150 (MQ=255)
                                                                            cccGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGc                                                                    >  2:655870/1‑150 (MQ=255)
                                                                             ccGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCt                                                                   <  1:1201866/150‑1 (MQ=255)
                                                                               ggAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCttt                                                                 <  1:4375117/150‑1 (MQ=255)
                                                                               ggAAGCACAATTAGCAGAACAGGCGCTCAGCAGCTGTCACATAGAAGCGTTAATTAAAATATGTGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCttt                                                                 <  2:3730762/150‑1 (MQ=255)
                                                                                gAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTg                                                                <  1:3149488/150‑1 (MQ=255)
                                                                                gAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTg                                                                <  1:4423364/150‑1 (MQ=255)
                                                                                 aaGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGa                                                               >  2:721690/1‑150 (MQ=255)
                                                                                 aaGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGa                                                               <  1:967951/150‑1 (MQ=255)
                                                                                  aGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGAc                                                              >  1:734847/1‑150 (MQ=255)
                                                                                    cacaATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCt                                                            >  2:1770936/1‑150 (MQ=255)
                                                                                     acaATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCtt                                                           <  2:1084232/150‑1 (MQ=255)
                                                                                       aaTTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCt                                                         >  1:548106/1‑150 (MQ=255)
                                                                                       aaTTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCt                                                         >  1:692720/1‑150 (MQ=255)
                                                                                            gCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTg                                                    <  2:4502819/150‑1 (MQ=255)
                                                                                              aGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCg                                                  >  1:3741813/1‑150 (MQ=255)
                                                                                              aGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCg                                                  >  2:2422554/1‑150 (MQ=255)
                                                                                              aGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCg                                                  >  1:4090926/1‑150 (MQ=255)
                                                                                              aGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCg                                                  >  2:4400244/1‑150 (MQ=255)
                                                                                              aGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCg                                                  >  1:3741620/1‑150 (MQ=255)
                                                                                               gAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGt                                                 >  2:789078/1‑150 (MQ=255)
                                                                                                   aGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGACAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCa                                             >  2:3708676/1‑150 (MQ=255)
                                                                                                   aGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGAAAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCa                                             >  2:4304902/1‑150 (MQ=255)
                                                                                                     gcgcTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAAt                                           <  1:2829314/150‑1 (MQ=255)
                                                                                                       gcTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATga                                         >  1:4503331/1‑150 (MQ=255)
                                                                                                         tCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGTAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATgaga                                       <  2:897020/150‑1 (MQ=255)
                                                                                                         tCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATgaga                                       <  2:897039/150‑1 (MQ=255)
                                                                                                         tCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGAAAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATgaga                                       >  2:2676421/1‑150 (MQ=255)
                                                                                                          ccGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGaa                                      <  1:4547736/150‑1 (MQ=255)
                                                                                                          ccGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGaa                                      <  2:1647621/150‑1 (MQ=255)
                                                                                                           cGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGaaa                                     >  2:1291674/1‑150 (MQ=255)
                                                                                                             cAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGacaaa                                   >  2:1992275/1‑150 (MQ=255)
                                                                                                                cGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCa                                >  2:388141/1‑150 (MQ=255)
                                                                                                                    cacaTAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAATGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTg                            >  1:1113312/1‑150 (MQ=255)
                                                                                                                         aGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTcgc                       >  2:4633491/1‑150 (MQ=255)
                                                                                                                         aGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTcgc                       <  1:4170840/150‑1 (MQ=255)
                                                                                                                           aaGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCgg                     >  2:328458/1‑150 (MQ=255)
                                                                                                                             gCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGt                   >  1:1808083/1‑150 (MQ=255)
                                                                                                                                  aattaaCATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGa              >  1:1008906/1‑150 (MQ=255)
                                                                                                                                     taaCATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTa           <  2:3619295/150‑1 (MQ=255)
                                                                                                                                      aaCATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTAt          <  1:42903/150‑1 (MQ=255)
                                                                                                                                       aCATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTAtt         >  1:1985087/1‑150 (MQ=255)
                                                                                                                                        cATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTCttt        >  2:1444531/1‑150 (MQ=255)
                                                                                                                                           atGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTTtct     <  2:3851432/150‑1 (MQ=255)
                                                                                                                                            tGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTTtctc    <  2:4139619/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
CTGCACGCAATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGAAAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTTTCTCAG  >  CP009273/2195304‑2195595

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATGCTGCACGCAATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGAAAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTTTCTCAGTAT  >  CP009273/2195301‑2195598
                                                                                                                                                    |                                                                                                                                                     
ATGCTGCACGCAATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATA                                                                                                                                                      >  SRR19973072.908909/1‑150 (MQ=60)
   CTGCACGCAATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATG                                                                                                                                                   >  SRR19973072.4523809/1‑150 (MQ=60)
   CTGCACGCAATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATG                                                                                                                                                   >  SRR19973072.864766/1‑150 (MQ=60)
   CTGCACGCAATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATG                                                                                                                                                   >  SRR19973072.4523813/1‑150 (MQ=60)
   CTGCACGCAATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACAGATGGGATAATG                                                                                                                                                   >  SRR19973072.4523789/1‑150 (MQ=60)
     GCACGCAATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGAC                                                                                                                                                 >  SRR19973072.1618128/1‑150 (MQ=60)
      CACGCAATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACA                                                                                                                                                <  SRR19973072.2611827/150‑1 (MQ=60)
      CACGCAATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACA                                                                                                                                                >  SRR19973072.2160266/1‑150 (MQ=60)
        CGCAATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGCTAATGACAAC                                                                                                                                              >  SRR19973072.2820311/1‑150 (MQ=60)
        CGCAATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAAC                                                                                                                                              >  SRR19973072.941179/1‑150 (MQ=60)
         GCAATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACT                                                                                                                                             <  SRR19973072.3869077/150‑1 (MQ=60)
           AATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTAC                                                                                                                                           <  SRR19973072.544702/150‑1 (MQ=60)
           AATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTAC                                                                                                                                           <  SRR19973072.544563/150‑1 (MQ=60)
           AATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTAC                                                                                                                                           <  SRR19973072.1856678/150‑1 (MQ=60)
               CACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCAC                                                                                                                                       <  SRR19973072.4370244/150‑1 (MQ=60)
               CACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCAC                                                                                                                                       <  SRR19973072.586070/150‑1 (MQ=60)
               CACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCAC                                                                                                                                       <  SRR19973072.586297/150‑1 (MQ=60)
                ACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACA                                                                                                                                      <  SRR19973072.1249426/150‑1 (MQ=60)
                 CTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACAT                                                                                                                                     <  SRR19973072.293840/150‑1 (MQ=60)
                  TCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATT                                                                                                                                    <  SRR19973072.3907031/150‑1 (MQ=60)
                   CTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTA                                                                                                                                   <  SRR19973072.2208848/150‑1 (MQ=60)
                    TTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTAT                                                                                                                                  <  SRR19973072.2405731/150‑1 (MQ=60)
                    TTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTAT                                                                                                                                  <  SRR19973072.2405175/150‑1 (MQ=60)
                        TTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTC                                                                                                                              <  SRR19973072.2456097/150‑1 (MQ=60)
                          CAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCG                                                                                                                            <  SRR19973072.1606023/150‑1 (MQ=60)
                           AGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGG                                                                                                                           >  SRR19973072.1253569/1‑150 (MQ=60)
                            GTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGA                                                                                                                          >  SRR19973072.2460629/1‑150 (MQ=60)
                              GGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAA                                                                                                                        <  SRR19973072.344580/150‑1 (MQ=60)
                                 ATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGA                                                                                                                     >  SRR19973072.1726380/1‑150 (MQ=60)
                                 ATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGA                                                                                                                     >  SRR19973072.1726370/1‑150 (MQ=60)
                                 ATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGA                                                                                                                     >  SRR19973072.44937/1‑150 (MQ=60)
                                  TTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAG                                                                                                                    <  SRR19973072.2821833/150‑1 (MQ=60)
                                  TTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAG                                                                                                                    <  SRR19973072.498147/150‑1 (MQ=60)
                                  TTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAG                                                                                                                    <  SRR19973072.4611605/150‑1 (MQ=60)
                                     CTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGT                                                                                                                 >  SRR19973072.2925168/1‑150 (MQ=60)
                                      TACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTC                                                                                                                >  SRR19973072.927771/1‑150 (MQ=60)
                                      TACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTC                                                                                                                >  SRR19973072.2542401/1‑150 (MQ=60)
                                       ACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCT                                                                                                               <  SRR19973072.1285634/150‑1 (MQ=60)
                                       ACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCT                                                                                                               <  SRR19973072.1286827/150‑1 (MQ=60)
                                       ACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCT                                                                                                               <  SRR19973072.1251338/150‑1 (MQ=60)
                                       ACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCT                                                                                                               <  SRR19973072.1251342/150‑1 (MQ=60)
                                       ACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCT                                                                                                               <  SRR19973072.4546652/150‑1 (MQ=60)
                                        CCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTG                                                                                                              <  SRR19973072.4139231/150‑1 (MQ=60)
                                        CCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTG                                                                                                              <  SRR19973072.1795344/150‑1 (MQ=60)
                                          AGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGA                                                                                                            >  SRR19973072.309843/1‑150 (MQ=60)
                                          AGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGA                                                                                                            >  SRR19973072.3626917/1‑150 (MQ=60)
                                          AGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGA                                                                                                            >  SRR19973072.1131040/1‑150 (MQ=60)
                                              GCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCT                                                                                                        <  SRR19973072.4059562/150‑1 (MQ=60)
                                              GCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCT                                                                                                        <  SRR19973072.2801621/150‑1 (MQ=60)
                                              GCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCT                                                                                                        <  SRR19973072.2801620/150‑1 (MQ=60)
                                                GGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGT                                                                                                      <  SRR19973072.1677808/150‑1 (MQ=60)
                                                   TTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATT                                                                                                   >  SRR19973072.270653/1‑150 (MQ=60)
                                                   TTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATT                                                                                                   >  SRR19973072.308308/1‑150 (MQ=60)
                                                   TTGAAGAGATCCTGAATGGCTGGAAATCCCCGGAAGCACAAATAGCAGAACAGGCGCTCCGCAGCGGACACATAGAAGCGTTAATTAACATAATGGATAATGACAACTACTCACAGTATCGTCCGGACAAGAGTGTCTGGAACCTGTATT                                                                                                   >  SRR19973072.3702850/1‑150 (MQ=60)
                                                    TGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTT                                                                                                  <  SRR19973072.1963711/150‑1 (MQ=60)
                                                    TGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGAAAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTT                                                                                                  >  SRR19973072.3503537/1‑150 (MQ=60)
                                                          GATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGC                                                                                            >  SRR19973072.2480354/1‑150 (MQ=60)
                                                             CCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACA                                                                                         >  SRR19973072.4148304/1‑150 (MQ=60)
                                                              CTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAG                                                                                        <  SRR19973072.193829/150‑1 (MQ=60)
                                                                     GCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGC                                                                                 >  SRR19973072.4376173/1‑150 (MQ=60)
                                                                     GCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGC                                                                                 >  SRR19973072.4376205/1‑150 (MQ=60)
                                                                       TGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGT                                                                               >  SRR19973072.2023827/1‑150 (MQ=60)
                                                                       TGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGT                                                                               >  SRR19973072.1323565/1‑150 (MQ=60)
                                                                        GGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTG                                                                              >  SRR19973072.3051887/1‑150 (MQ=60)
                                                                         GAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGA                                                                             <  SRR19973072.2562629/150‑1 (MQ=60)
                                                                         GAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGA                                                                             <  SRR19973072.2562595/150‑1 (MQ=60)
                                                                         GAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGA                                                                             >  SRR19973072.555388/1‑150 (MQ=60)
                                                                          AACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAG                                                                            <  SRR19973072.2635924/150‑1 (MQ=60)
                                                                          AACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAG                                                                            <  SRR19973072.2635952/150‑1 (MQ=60)
                                                                           ACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGA                                                                           <  SRR19973072.3068364/150‑1 (MQ=60)
                                                                           ACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGA                                                                           <  SRR19973072.3068334/150‑1 (MQ=60)
                                                                            CTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGAT                                                                          >  SRR19973072.2830160/1‑150 (MQ=60)
                                                                            CTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGAT                                                                          <  SRR19973072.2502445/150‑1 (MQ=60)
                                                                            CTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGAT                                                                          <  SRR19973072.1904570/150‑1 (MQ=60)
                                                                            CTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGACACAGTTGCCGCGTGAGAT                                                                          >  SRR19973072.3375644/1‑150 (MQ=60)
                                                                             TCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATG                                                                         >  SRR19973072.2544601/1‑150 (MQ=60)
                                                                                CCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCT                                                                      >  SRR19973072.1102382/1‑150 (MQ=60)
                                                                                CCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCT                                                                      <  SRR19973072.1204349/150‑1 (MQ=60)
                                                                                CCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCT                                                                      <  SRR19973072.1204421/150‑1 (MQ=60)
                                                                                  GGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTT                                                                    >  SRR19973072.2902200/1‑150 (MQ=60)
                                                                                  GGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTT                                                                    <  SRR19973072.4384391/150‑1 (MQ=60)
                                                                                  GGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTT                                                                    <  SRR19973072.4384354/150‑1 (MQ=60)
                                                                                  GGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTT                                                                    <  SRR19973072.4384309/150‑1 (MQ=60)
                                                                                   GAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTG                                                                   <  SRR19973072.4432684/150‑1 (MQ=60)
                                                                                   GAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTG                                                                   <  SRR19973072.3156135/150‑1 (MQ=60)
                                                                                    AAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGA                                                                  <  SRR19973072.970066/150‑1 (MQ=60)
                                                                                     AGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGAC                                                                 >  SRR19973072.736425/1‑150 (MQ=60)
                                                                                     AGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGAC                                                                 >  SRR19973072.736393/1‑150 (MQ=60)
                                                                                      GCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTAACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACC                                                                <  SRR19973072.4199123/150‑1 (MQ=60)
                                                                                          AATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCT                                                            >  SRR19973072.2955255/1‑150 (MQ=60)
                                                                                          AATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCT                                                            >  SRR19973072.694243/1‑150 (MQ=60)
                                                                                          AATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCT                                                            >  SRR19973072.549399/1‑150 (MQ=60)
                                                                                           ATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTG                                                           <  SRR19973072.1290271/150‑1 (MQ=60)
                                                                                                CAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGC                                                      >  SRR19973072.1803554/1‑150 (MQ=60)
                                                                                                 AGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCG                                                     >  SRR19973072.3749684/1‑150 (MQ=60)
                                                                                                 AGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCG                                                     >  SRR19973072.3749491/1‑150 (MQ=60)
                                                                                                 AGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCG                                                     >  SRR19973072.4099508/1‑150 (MQ=60)
                                                                                                 AGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCG                                                     >  SRR19973072.3749659/1‑150 (MQ=60)
                                                                                                        GCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAAT                                              <  SRR19973072.2835371/150‑1 (MQ=60)
                                                                                                         CGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATG                                             <  SRR19973072.655279/150‑1 (MQ=60)
                                                                                                          GCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGA                                            >  SRR19973072.4512842/1‑150 (MQ=60)
                                                                                                           CTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAG                                           >  SRR19973072.3795832/1‑150 (MQ=60)
                                                                                                           CTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAG                                           >  SRR19973072.2709809/1‑150 (MQ=60)
                                                                                                             CCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAA                                         <  SRR19973072.4557324/150‑1 (MQ=60)
                                                                                                               TCAGCGGTCACATAGAAGCGTTAATTAACATATTGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGATTTGACCTTCTGGCTGCGTATCAATGAGAAAA                                       <  SRR19973072.415323/150‑1 (MQ=60)
                                                                                                                   CGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCA                                   >  SRR19973072.1206209/1‑150 (MQ=60)
                                                                                                                    GGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCAT                                  >  SRR19973072.3719111/1‑150 (MQ=60)
                                                                                                                    GGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCAT                                  >  SRR19973072.4373672/1‑150 (MQ=60)
                                                                                                                       CACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAATGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTG                               >  SRR19973072.1115663/1‑150 (MQ=60)
                                                                                                                       CACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTG                               >  SRR19973072.1267858/1‑150 (MQ=60)
                                                                                                                          ATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTC                            >  SRR19973072.3669466/1‑150 (MQ=60)
                                                                                                                            AGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGC                          <  SRR19973072.4621663/150‑1 (MQ=60)
                                                                                                                            AGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGC                          <  SRR19973072.4179574/150‑1 (MQ=60)
                                                                                                                                GCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGT                      >  SRR19973072.1812026/1‑150 (MQ=60)
                                                                                                                                   TTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAG                   <  SRR19973072.2415679/150‑1 (MQ=60)
                                                                                                                                     AATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGA                 >  SRR19973072.1011104/1‑150 (MQ=60)
                                                                                                                                       TTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACT               <  SRR19973072.3719945/150‑1 (MQ=60)
                                                                                                                                        TAACATATGGGACAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTA              <  SRR19973072.793836/150‑1 (MQ=60)
                                                                                                                                         AACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTAT             <  SRR19973072.3120884/150‑1 (MQ=60)
                                                                                                                                         AACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTAT             <  SRR19973072.43024/150‑1 (MQ=60)
                                                                                                                                          ACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATT            >  SRR19973072.3242554/1‑150 (MQ=60)
                                                                                                                                          ACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATT            >  SRR19973072.1989351/1‑150 (MQ=60)
                                                                                                                                              ATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTTTCT        >  SRR19973072.4115175/1‑150 (MQ=60)
                                                                                                                                                 GGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTTTCTCAG     >  SRR19973072.4152591/1‑150 (MQ=60)
                                                                                                                                                    TAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTTTCTCAGTAT  <  SRR19973072.2732659/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
ATGCTGCACGCAATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGAAAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTTTCTCAGTAT  >  CP009273/2195301‑2195598

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑