Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F78 I0 R1 626 241.3 10389380 77.7% 8072548 149.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,195,449 A→T 92.9% E564D (GAA→GAT)  yehI → DUF4132 domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,195,4490AT92.9% 774.4 / 38.5 225E564D (GAA→GAT) yehIDUF4132 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (9/7);  new base T (106/103);  total (115/110)
Fisher's exact test for biased strand distribution p-value = 7.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

Only 100 of 227 total aligned reads displayed.

TGCTGCACGCAATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGAAAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTTTCTCAG  >  CP009273/2195302‑2195595
                                                                                                                                                   |                                                                                                                                                  
 gctgcACGCAATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAAt                                                                                                                                                 <  1:2022406/150‑1 (MQ=255)
  ctgcACGCAATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATg                                                                                                                                                <  1:877848/150‑1 (MQ=255)
   tgcACGCAATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGa                                                                                                                                               >  1:3098650/1‑150 (MQ=255)
    gcACGCAATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGAc                                                                                                                                              <  2:751256/150‑1 (MQ=255)
     cACGCAATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACa                                                                                                                                             >  1:697244/1‑150 (MQ=255)
             tCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTca                                                                                                                                     >  2:2116805/1‑150 (MQ=255)
             tCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTca                                                                                                                                     >  2:3766739/1‑150 (MQ=255)
             tCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTca                                                                                                                                     >  2:1897444/1‑150 (MQ=255)
               aCTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTcaca                                                                                                                                   >  2:3520712/1‑150 (MQ=255)
                 tctTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACAtt                                                                                                                                 <  1:1292432/150‑1 (MQ=255)
                 tctTTGTTCAGAGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCATCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACAtt                                                                                                                                 <  2:4864959/150‑1 (MQ=255)
                    ttGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATc                                                                                                                              <  1:1705657/150‑1 (MQ=255)
                           gTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGa                                                                                                                       <  2:821855/150‑1 (MQ=255)
                             ggCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGaaa                                                                                                                     <  2:2641755/150‑1 (MQ=255)
                                ataactacCAGGGTCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTAACATAGAATCGTTAATAAACATATGGGATAATGACAACTACACACATTATCGTCCGGAAAaga                                                                                                                  >  1:4357938/4‑150 (MQ=255)
                                aTTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAaga                                                                                                                  <  1:4444459/150‑1 (MQ=255)
                                aTTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAaga                                                                                                                  >  1:2893662/1‑150 (MQ=255)
                                 ttACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAagag                                                                                                                 <  2:780795/150‑1 (MQ=255)
                                  tactacCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGAAAATGACAACTACTCACATTATCGTCCGGAAAAGAgt                                                                                                                <  2:2384619/150‑1 (MQ=255)
                                  tactacCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCGCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGAAAATGACAACTACTCACATTATCGTCCGGAAAAGAgt                                                                                                                <  2:4965405/150‑1 (MQ=255)
                                     tacCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTc                                                                                                             <  2:3862905/150‑1 (MQ=255)
                                     tacCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTc                                                                                                             >  2:453488/1‑150 (MQ=255)
                                     tacCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTc                                                                                                             >  2:4102480/1‑150 (MQ=255)
                                        cAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGACAAGAGTGTCTgg                                                                                                          >  2:3168599/1‑150 (MQ=255)
                                         aGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGa                                                                                                         >  1:165790/1‑150 (MQ=255)
                                            ggCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAAcc                                                                                                      <  1:1155636/150‑1 (MQ=255)
                                            ggCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAAcc                                                                                                      <  1:2655915/150‑1 (MQ=255)
                                            ggCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAAcc                                                                                                      <  2:418405/150‑1 (MQ=255)
                                                    gAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGtattta                                                                                              >  1:982961/1‑150 (MQ=255)
                                                    gAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGtattta                                                                                              >  2:3207135/1‑150 (MQ=255)
                                                    gAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGtattta                                                                                              <  1:4613253/150‑1 (MQ=255)
                                                     aaGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGtatttat                                                                                             <  1:2815593/150‑1 (MQ=255)
                                                      agagATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGtatttatt                                                                                            >  2:2164541/1‑150 (MQ=255)
                                                      agagATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGtatttatt                                                                                            >  2:3582759/1‑150 (MQ=255)
                                                      agagATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGtatttatt                                                                                            <  1:5144171/150‑1 (MQ=255)
                                                        agaTCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTgg                                                                                          >  2:4271667/1‑150 (MQ=255)
                                                          aTCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGca                                                                                        <  2:1665318/150‑1 (MQ=255)
                                                            ccTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGcaca                                                                                      >  2:2596302/1‑150 (MQ=255)
                                                            ccTCAATGGCTGGAACTCCCCGGAAGCACAAATCGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATAATCGACCGGAAAAGAGGGTCTGGAACCGGTATTAATTGGcaca                                                                                      >  2:5174254/1‑150 (MQ=255)
                                                              tGACTGGCTGGAACTCCCCGGAAGCACAACTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGt                                                                                    <  2:4373667/150‑1 (MQ=255)
                                                                aaTGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTg                                                                                  >  1:4803879/1‑150 (MQ=255)
                                                                  tGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGACAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGcc                                                                                >  2:3132980/1‑150 (MQ=255)
                                                                  tGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGcc                                                                                <  1:4160643/150‑1 (MQ=255)
                                                                  tGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGcc                                                                                <  1:3932627/150‑1 (MQ=255)
                                                                   ggCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCcg                                                                               >  2:344066/1‑150 (MQ=255)
                                                                      tGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGt                                                                            <  2:1648947/150‑1 (MQ=255)
                                                                      tGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGAAAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGt                                                                            >  2:2079362/1‑150 (MQ=255)
                                                                         aaCTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTgag                                                                         <  1:78607/150‑1 (MQ=255)
                                                                         aaCTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTgag                                                                         >  1:3427449/1‑150 (MQ=255)
                                                                          aCTCCCCGGAAGCACACTTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTgaga                                                                        <  2:1576630/150‑1 (MQ=255)
                                                                           cTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGAt                                                                       <  1:3908720/150‑1 (MQ=255)
                                                                           cTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGAt                                                                       >  2:1301488/1‑150 (MQ=255)
                                                                            tCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATg                                                                      <  2:4471676/150‑1 (MQ=255)
                                                                             ccccGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATgg                                                                     >  2:3973246/1‑150 (MQ=255)
                                                                              cccGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGc                                                                    <  1:3520861/150‑1 (MQ=255)
                                                                              cccGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGc                                                                    <  1:3520693/150‑1 (MQ=255)
                                                                                cGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGAAAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCtt                                                                  <  1:1963198/150‑1 (MQ=255)
                                                                                 ggAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCttt                                                                 <  2:1993333/150‑1 (MQ=255)
                                                                                 ggAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCttt                                                                 >  1:3306389/1‑150 (MQ=255)
                                                                                   aaGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGa                                                               >  1:100803/1‑150 (MQ=255)
                                                                                   aaGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGa                                                               >  2:3003995/1‑150 (MQ=255)
                                                                                    aGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGAc                                                              >  1:812290/1‑150 (MQ=255)
                                                                                       acaATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCtt                                                           <  2:2767172/150‑1 (MQ=255)
                                                                                        caATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTc                                                          <  1:248827/150‑1 (MQ=255)
                                                                                        caATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTc                                                          <  2:3277670/150‑1 (MQ=255)
                                                                                         aaTTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCt                                                         <  1:2984803/150‑1 (MQ=255)
                                                                                          aTTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTg                                                        >  1:3151029/1‑150 (MQ=255)
                                                                                          aTTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTg                                                        >  1:2346085/1‑150 (MQ=255)
                                                                                          aTTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTg                                                        >  1:4421710/1‑150 (MQ=255)
                                                                                               cAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGc                                                   <  2:728178/150‑1 (MQ=255)
                                                                                                  aaCAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTa                                                >  1:2710299/1‑150 (MQ=255)
                                                                                                     aGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCa                                             >  2:937964/1‑150 (MQ=255)
                                                                                                     aGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCa                                             >  2:3110588/1‑150 (MQ=255)
                                                                                                     aGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCa                                             >  2:218471/1‑150 (MQ=255)
                                                                                                       gcgcTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAAt                                           <  1:2538797/150‑1 (MQ=255)
                                                                                                        cgcTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATg                                          <  1:4536230/150‑1 (MQ=255)
                                                                                                         gcTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATga                                         <  1:4684310/150‑1 (MQ=255)
                                                                                                         gcTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATga                                         >  2:1233248/1‑150 (MQ=255)
                                                                                                           tCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATgaga                                       >  2:5092171/1‑150 (MQ=255)
                                                                                                           tCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATgaga                                       >  2:1942441/1‑150 (MQ=255)
                                                                                                             cGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGAAAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGaaa                                     <  1:62189/150‑1 (MQ=255)
                                                                                                              gCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGaaaa                                    <  2:894519/150‑1 (MQ=255)
                                                                                                               cAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACAGTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGaaaaa                                   >  2:3608446/1‑150 (MQ=255)
                                                                                                                aGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAg                                  >  2:2964802/1‑150 (MQ=255)
                                                                                                                   ggTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCAt                               >  2:4980592/1‑150 (MQ=255)
                                                                                                                    gTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTCTTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATc                              >  2:1705729/1‑150 (MQ=255)
                                                                                                                      cacaTAGAAGCGTTAATTAACATATGGGATAATGACAACGACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTg                            <  2:2698603/150‑1 (MQ=255)
                                                                                                                         aTAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTc                         >  1:2492883/1‑150 (MQ=255)
                                                                                                                          tAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTcg                        >  2:4677804/1‑150 (MQ=255)
                                                                                                                           aGAAGCGTTAATTAACATATGGGAAAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTcgc                       >  1:3642208/1‑150 (MQ=255)
                                                                                                                           aGAAGCGTTAATTAACATATGGGAAAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTcgc                       >  1:2753113/1‑150 (MQ=255)
                                                                                                                                 gTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGa                 >  2:1133386/1‑150 (MQ=255)
                                                                                                                                  ttaattaaCATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAg                >  1:547034/1‑150 (MQ=255)
                                                                                                                                  ttaattaaCATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAg                <  1:3940624/150‑1 (MQ=255)
                                                                                                                                   taattaaCATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAgg               >  2:3035346/1‑150 (MQ=255)
                                                                                                                                   taattaaCATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAgg               >  1:2319701/1‑150 (MQ=255)
                                                                                                                                          cATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTAttt        >  2:305701/1‑150 (MQ=255)
                                                                                                                                          cATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTAttt        <  1:1513144/150‑1 (MQ=255)
                                                                                                                                           atatGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTAtttt       <  2:2256730/150‑1 (MQ=255)
                                                                                                                                                ggATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTTTCTCAg  <  1:1041919/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TGCTGCACGCAATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGAAAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTTTCTCAG  >  CP009273/2195302‑2195595

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GATGCTGCACGCAATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGAAAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTTTCTCAGTA  >  CP009273/2195300‑2195597
                                                                                                                                                     |                                                                                                                                                    
GATGCTGCACGCAATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGAT                                                                                                                                                      <  SRR19973083.3978768/150‑1 (MQ=60)
  TGCTGCACGCAATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAA                                                                                                                                                    >  SRR19973083.1996494/1‑150 (MQ=60)
  NGCTGCACGCAATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAAAAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAA                                                                                                                                                    >  SRR19973083.4648063/1‑150 (MQ=60)
   GCTGCACGCAATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAAT                                                                                                                                                   <  SRR19973083.2032739/150‑1 (MQ=60)
   GCTGCACGCAATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAAT                                                                                                                                                   <  SRR19973083.3322978/150‑1 (MQ=60)
    CTGCACGCAATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATG                                                                                                                                                  >  SRR19973083.2215958/1‑150 (MQ=60)
    CTGCACGCAATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATG                                                                                                                                                  <  SRR19973083.881957/150‑1 (MQ=60)
     TGCACGCAATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGA                                                                                                                                                 >  SRR19973083.3113512/1‑150 (MQ=60)
     TGCACGCAATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGA                                                                                                                                                 >  SRR19973083.3113497/1‑150 (MQ=60)
       CACGCAATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACA                                                                                                                                               >  SRR19973083.700719/1‑150 (MQ=60)
       CACGCAATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACA                                                                                                                                               >  SRR19973083.700863/1‑150 (MQ=60)
       CACGCAATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGAAAATGACA                                                                                                                                               >  SRR19973083.626431/1‑150 (MQ=60)
               TCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCA                                                                                                                                       <  SRR19973083.26168/150‑1 (MQ=60)
                  CTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACAT                                                                                                                                    <  SRR19973083.1359151/150‑1 (MQ=60)
                   TCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATT                                                                                                                                   <  SRR19973083.1298667/150‑1 (MQ=60)
                   TATTTGTTCTGTGGAATTACTACCAGTGGCGTGTTGAAGAGATCCTGAATGGCTGGAACTCCAAGGAAGCAAAATTTGCAGACCAGGCTATCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATT                                                                                                                                   <  SRR19973083.1298867/150‑1 (MQ=60)
                    CTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTA                                                                                                                                  <  SRR19973083.2541604/150‑1 (MQ=60)
                      TTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATC                                                                                                                                <  SRR19973083.1713998/150‑1 (MQ=60)
                      TTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATC                                                                                                                                <  SRR19973083.1714052/150‑1 (MQ=60)
                      TTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATC                                                                                                                                <  SRR19973083.360746/150‑1 (MQ=60)
                      TTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATC                                                                                                                                >  SRR19973083.2391240/1‑150 (MQ=60)
                       TGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCG                                                                                                                               <  SRR19973083.954291/150‑1 (MQ=60)
                         TTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTC                                                                                                                             <  SRR19973083.4910942/150‑1 (MQ=60)
                                  ATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGA                                                                                                                    >  SRR19973083.2907851/1‑150 (MQ=60)
                                  ATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGA                                                                                                                    <  SRR19973083.4463786/150‑1 (MQ=60)
                                  ATAACTACCAGGGTCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTAACATAGAATCGTTAATAAACATATGGGATAATGACAACTACACACATTATCGTCCGGAAAAGA                                                                                                                    >  SRR19973083.4376849/1‑150 (MQ=60)
                                    TACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGT                                                                                                                  >  SRR19973083.844285/1‑150 (MQ=60)
                                    TACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGT                                                                                                                  <  SRR19973083.2758276/150‑1 (MQ=60)
                                           AGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGA                                                                                                           >  SRR19973083.2794400/1‑150 (MQ=60)
                                           AGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGA                                                                                                           >  SRR19973083.166754/1‑150 (MQ=60)
                                              GGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCCCATTATCGTCCGGAAAAGAGTGTCTGGAACC                                                                                                        <  SRR19973083.1970436/150‑1 (MQ=60)
                                              GGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACC                                                                                                        <  SRR19973083.2669248/150‑1 (MQ=60)
                                              GGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACC                                                                                                        <  SRR19973083.2669269/150‑1 (MQ=60)
                                              GGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACC                                                                                                        <  SRR19973083.4568215/150‑1 (MQ=60)
                                              GGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACC                                                                                                        <  SRR19973083.1161392/150‑1 (MQ=60)
                                                    TTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATT                                                                                                  <  SRR19973083.3323207/150‑1 (MQ=60)
                                                     TGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTT                                                                                                 <  SRR19973083.3761747/150‑1 (MQ=60)
                                                     TGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTT                                                                                                 <  SRR19973083.1520558/150‑1 (MQ=60)
                                                      GAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATATAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTA                                                                                                <  SRR19973083.2347613/150‑1 (MQ=60)
                                                      GAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTA                                                                                                >  SRR19973083.987841/1‑150 (MQ=60)
                                                      GAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTA                                                                                                <  SRR19973083.4633167/150‑1 (MQ=60)
                                                       AAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTAT                                                                                               <  SRR19973083.2829538/150‑1 (MQ=60)
                                                        AGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATT                                                                                              <  SRR19973083.5166029/150‑1 (MQ=60)
                                                          AGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGG                                                                                            >  SRR19973083.473577/1‑150 (MQ=60)
                                                          AGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGG                                                                                            >  SRR19973083.3095810/1‑150 (MQ=60)
                                                                  AATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTG                                                                                    >  SRR19973083.4824433/1‑150 (MQ=60)
                                                                    TGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCC                                                                                  <  SRR19973083.3950387/150‑1 (MQ=60)
                                                                    TGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCC                                                                                  <  SRR19973083.4178079/150‑1 (MQ=60)
                                                                    TGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCC                                                                                  <  SRR19973083.4178991/150‑1 (MQ=60)
                                                                    TGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCC                                                                                  <  SRR19973083.3950135/150‑1 (MQ=60)
                                                                     GGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCG                                                                                 >  SRR19973083.1598161/1‑150 (MQ=60)
                                                                           AACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAG                                                                           >  SRR19973083.3443224/1‑150 (MQ=60)
                                                                           AACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAG                                                                           <  SRR19973083.79171/150‑1 (MQ=60)
                                                                           AACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAG                                                                           <  SRR19973083.79182/150‑1 (MQ=60)
                                                                           AACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAG                                                                           <  SRR19973083.345724/150‑1 (MQ=60)
                                                                           AACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAG                                                                           <  SRR19973083.2969985/150‑1 (MQ=60)
                                                                           AACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGAAAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAG                                                                           >  SRR19973083.1983719/1‑150 (MQ=60)
                                                                            ACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGA                                                                          <  SRR19973083.5180473/150‑1 (MQ=60)
                                                                             CTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGAT                                                                         <  SRR19973083.3926115/150‑1 (MQ=60)
                                                                             CTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGAGGTAACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGAT                                                                         <  SRR19973083.3926214/150‑1 (MQ=60)
                                                                                CCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGC                                                                      <  SRR19973083.3536841/150‑1 (MQ=60)
                                                                                CCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGC                                                                      <  SRR19973083.3536860/150‑1 (MQ=60)
                                                                                CCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGC                                                                      <  SRR19973083.3537009/150‑1 (MQ=60)
                                                                                  CGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGAAAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTT                                                                    <  SRR19973083.1973226/150‑1 (MQ=60)
                                                                                   GGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTT                                                                   >  SRR19973083.3321917/1‑150 (MQ=60)
                                                                                   GGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGAAAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTT                                                                   >  SRR19973083.1111383/1‑150 (MQ=60)
                                                                                     AAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGA                                                                 >  SRR19973083.101475/1‑150 (MQ=60)
                                                                                      AGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGAC                                                                >  SRR19973083.816165/1‑150 (MQ=60)
                                                                                          CAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTC                                                            <  SRR19973083.250394/150‑1 (MQ=60)
                                                                                          CAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTC                                                            <  SRR19973083.250165/150‑1 (MQ=60)
                                                                                           AATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCT                                                           <  SRR19973083.2999174/150‑1 (MQ=60)
                                                                                           AATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCT                                                           <  SRR19973083.2999264/150‑1 (MQ=60)
                                                                                            ATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTG                                                          <  SRR19973083.2887637/150‑1 (MQ=60)
                                                                                            ATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTG                                                          >  SRR19973083.4440956/1‑150 (MQ=60)
                                                                                            ATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTG                                                          >  SRR19973083.2358155/1‑150 (MQ=60)
                                                                                            ATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTG                                                          >  SRR19973083.3166075/1‑150 (MQ=60)
                                                                                             TTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGG                                                         <  SRR19973083.500011/150‑1 (MQ=60)
                                                                                               AGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCT                                                       <  SRR19973083.2503326/150‑1 (MQ=60)
                                                                                                GCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTG                                                      <  SRR19973083.3998695/150‑1 (MQ=60)
                                                                                                  AGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCG                                                    <  SRR19973083.4473076/150‑1 (MQ=60)
                                                                                                  AGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAAGGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCG                                                    <  SRR19973083.4473040/150‑1 (MQ=60)
                                                                                                    AACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTA                                                  >  SRR19973083.637895/1‑150 (MQ=60)
                                                                                                    AACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTA                                                  >  SRR19973083.2723858/1‑150 (MQ=60)
                                                                                                    AACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTA                                                  >  SRR19973083.93682/1‑150 (MQ=60)
                                                                                                         GCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAAT                                             <  SRR19973083.2551674/150‑1 (MQ=60)
                                                                                                          CGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATG                                            <  SRR19973083.4555903/150‑1 (MQ=60)
                                                                                                           GCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGA                                           <  SRR19973083.4704418/150‑1 (MQ=60)
                                                                                                           GCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGA                                           <  SRR19973083.4450659/150‑1 (MQ=60)
                                                                                                               CGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGAAAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAA                                       <  SRR19973083.2693438/150‑1 (MQ=60)
                                                                                                               CGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGAAAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAA                                       <  SRR19973083.62659/150‑1 (MQ=60)
                                                                                                                 CAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAA                                     <  SRR19973083.1260576/150‑1 (MQ=60)
                                                                                                                     GGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCAT                                 >  SRR19973083.4340782/1‑150 (MQ=60)
                                                                                                                           ATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTC                           >  SRR19973083.2505598/1‑150 (MQ=60)
                                                                                                                             AGAAGCGTTAATTAACATATGGGAAAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGC                         >  SRR19973083.2766820/1‑150 (MQ=60)
                                                                                                                             AGAAGCGTTAATTAACATATGGGAAAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGC                         >  SRR19973083.3658870/1‑150 (MQ=60)
                                                                                                                              GAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCG                        >  SRR19973083.3170120/1‑150 (MQ=60)
                                                                                                                                 GCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGT                     <  SRR19973083.1023558/150‑1 (MQ=60)
                                                                                                                                    TTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAG                  >  SRR19973083.549770/1‑150 (MQ=60)
                                                                                                                                    TTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAG                  >  SRR19973083.549961/1‑150 (MQ=60)
                                                                                                                                    TTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAG                  <  SRR19973083.3958168/150‑1 (MQ=60)
                                                                                                                                     TAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGG                 >  SRR19973083.2331602/1‑150 (MQ=60)
                                                                                                                                         TAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTA             >  SRR19973083.4436115/1‑150 (MQ=60)
                                                                                                                                            CATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTT          <  SRR19973083.1520765/150‑1 (MQ=60)
                                                                                                                                                  GGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTTTCTCAG    <  SRR19973083.3657267/150‑1 (MQ=60)
                                                                                                                                                  GGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTTTCTCAG    <  SRR19973083.1047156/150‑1 (MQ=60)
                                                                                                                                                   GATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTTTCTCAGT   >  SRR19973083.5068469/1‑150 (MQ=60)
                                                                                                                                                   GATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTTTCTCAGT   >  SRR19973083.5066639/1‑150 (MQ=60)
                                                                                                                                                   GATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTTTCTCAGT   >  SRR19973083.4825224/1‑150 (MQ=60)
                                                                                                                                                    ATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTTTCTCAGTA  >  SRR19973083.107458/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
GATGCTGCACGCAATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGAAAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTTTCTCAGTA  >  CP009273/2195300‑2195597

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑