Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F121 I0 R1 856 273.6 9081716 94.1% 8545894 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,195,449 A→T 84.4% E564D (GAA→GAT)  yehI → DUF4132 domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,195,4490AT84.4% 612.7 / 94.6 212E564D (GAA→GAT) yehIDUF4132 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (19/14);  new base T (68/111);  total (87/125)
Fisher's exact test for biased strand distribution p-value = 5.29e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 216 total aligned reads displayed.

TGCTGCACGCAATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGAAAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTTTCTCAGTAT  >  CP009273/2195302‑2195598
                                                                                                                                                   |                                                                                                                                                     
     cACGCAATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACa                                                                                                                                                >  2:4433741/1‑150 (MQ=255)
         cAATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAacta                                                                                                                                            <  2:3566135/150‑1 (MQ=255)
           aTTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAactact                                                                                                                                          <  2:2754018/150‑1 (MQ=255)
              cACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGAAAATGACAACTACTcac                                                                                                                                       >  2:2585689/1‑150 (MQ=255)
              cACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGAAAATGACAACTACTcac                                                                                                                                       <  2:3434230/150‑1 (MQ=255)
               aCTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGAAAATGACAACTACTcaca                                                                                                                                      >  1:1280497/1‑150 (MQ=255)
                   tttGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTATTTAACATATGGGATAATGACAACTACTCACATTAt                                                                                                                                  <  1:1004247/150‑1 (MQ=255)
                        tCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTcc                                                                                                                             <  2:2664708/150‑1 (MQ=255)
                         cAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCg                                                                                                                            >  1:3222612/1‑150 (MQ=255)
                           gTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGAAAATGACAACTACTCACATTATCGTCCGGa                                                                                                                          >  2:3319263/1‑150 (MQ=255)
                                aTTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAaga                                                                                                                     <  1:4055690/150‑1 (MQ=255)
                                 ttACTACCTGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAagag                                                                                                                    <  2:4161024/150‑1 (MQ=255)
                                 ttACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAagag                                                                                                                    <  1:4465430/150‑1 (MQ=255)
                                  tactacCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAgt                                                                                                                   <  2:2401529/150‑1 (MQ=255)
                                  tactacCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAgt                                                                                                                   >  2:755553/1‑150 (MQ=255)
                                   actacCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAgtg                                                                                                                  >  1:1934143/1‑150 (MQ=255)
                                   actacCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAgtg                                                                                                                  <  1:575096/150‑1 (MQ=255)
                                     tacCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTc                                                                                                                <  2:2075092/150‑1 (MQ=255)
                                       ccAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTg                                                                                                              <  1:585212/150‑1 (MQ=255)
                                         aGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGa                                                                                                            <  1:1125411/150‑1 (MQ=255)
                                         aGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTCAATTAACATATGGGAAAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGa                                                                                                            <  1:1028633/150‑1 (MQ=255)
                                          ggggCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGaa                                                                                                           >  2:3032243/1‑150 (MQ=255)
                                             gCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCt                                                                                                        <  2:715743/150‑1 (MQ=255)
                                             gCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCt                                                                                                        <  2:3517347/150‑1 (MQ=255)
                                             gCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCt                                                                                                        <  2:2502178/150‑1 (MQ=255)
                                                ggTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGta                                                                                                     <  2:3984575/150‑1 (MQ=255)
                                                ggTTGAAGAGATCCTGAAGGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTACCATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGta                                                                                                     <  2:3984426/150‑1 (MQ=255)
                                                  ttGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGtatt                                                                                                   <  1:861372/150‑1 (MQ=255)
                                                     aaGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGAAAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGtatttat                                                                                                <  2:637747/150‑1 (MQ=255)
                                                       gagaTCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGAAAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTg                                                                                              >  1:3675050/1‑150 (MQ=255)
                                                        agaTCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGAAAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTgg                                                                                             <  1:2992243/150‑1 (MQ=255)
                                                         gaTCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGc                                                                                            <  1:3770106/150‑1 (MQ=255)
                                                            ccTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGcaca                                                                                         >  2:2851662/1‑150 (MQ=255)
                                                               gAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGtt                                                                                      >  1:3289421/1‑150 (MQ=255)
                                                                 aTGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACAGAGACGCGTTAATGAACCTATGGGATAATGACAACTACTCACAGTATCGTCCGGACAAGAGAGTCTGGACCCTGGATTTATTGGCACAGTTGc                                                                                    >  2:4000940/1‑150 (MQ=255)
                                                                  tGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTTACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGcc                                                                                   <  2:2861504/150‑1 (MQ=255)
                                                                   ggCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCcg                                                                                  <  2:188056/150‑1 (MQ=255)
                                                                      tGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGACGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGt                                                                               >  2:3109170/1‑150 (MQ=255)
                                                                      tGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGACACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGt                                                                               >  2:1530438/1‑150 (MQ=255)
                                                                        gAACTCCCCGGAAGCACAATGAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTga                                                                             <  1:594059/150‑1 (MQ=255)
                                                                         aaCTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTgag                                                                            <  2:3595790/150‑1 (MQ=255)
                                                                                cGGAAGCAAAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGTGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCtt                                                                     <  2:2323820/150‑1 (MQ=255)
                                                                                  gAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTg                                                                   <  2:1101588/150‑1 (MQ=255)
                                                                                  gAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTg                                                                   >  1:1229045/1‑150 (MQ=255)
                                                                                  gAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATGATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTg                                                                   <  2:1317491/150‑1 (MQ=255)
                                                                                     gCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCCCATAGAAGCGTTAATTAACATATGGGAAAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGAcc                                                                >  1:3165577/1‑150 (MQ=255)
                                                                                     gCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGAAAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGAcc                                                                >  1:897537/1‑150 (MQ=255)
                                                                                      cacaATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCt                                                               <  2:2976547/150‑1 (MQ=255)
                                                                                       acaATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCtt                                                              >  1:975821/1‑150 (MQ=255)
                                                                                       acaATTAGCAGAACAGGCGCTCCGCAGCGGTCACAGAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCtt                                                              <  2:1009155/150‑1 (MQ=255)
                                                                                        caATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGAAAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTc                                                             <  1:406467/150‑1 (MQ=255)
                                                                                           ttAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTgg                                                          <  2:3711135/150‑1 (MQ=255)
                                                                                             aGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCt                                                        <  1:3742279/150‑1 (MQ=255)
                                                                                                aGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCg                                                     >  1:4497339/1‑150 (MQ=255)
                                                                                                  aaCAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTa                                                   <  1:1484141/150‑1 (MQ=255)
                                                                                                    cAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATc                                                 <  1:1776030/150‑1 (MQ=255)
                                                                                                    cAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATc                                                 <  1:2023212/150‑1 (MQ=255)
                                                                                                      ggCGCTCCGCAGCGGTCACAGAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCaa                                               >  2:1163106/1‑150 (MQ=255)
                                                                                                        cgcTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATg                                             <  2:2392662/150‑1 (MQ=255)
                                                                                                           tCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATgaga                                          <  2:197482/150‑1 (MQ=255)
                                                                                                            ccGCAGCGGTCACATAGAATCTTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGaa                                         <  1:4439680/150‑1 (MQ=255)
                                                                                                            ccGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGaa                                         <  2:1430497/150‑1 (MQ=255)
                                                                                                               cAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGaaaaa                                      >  2:323281/1‑150 (MQ=255)
                                                                                                               cAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGaaaaa                                      <  2:3126070/150‑1 (MQ=255)
                                                                                                                aGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAg                                     >  1:3362268/1‑150 (MQ=255)
                                                                                                                aGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAg                                     >  1:2917257/1‑150 (MQ=255)
                                                                                                                 gCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGc                                    <  2:1555608/150‑1 (MQ=255)
                                                                                                                  cGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCa                                   <  2:1480318/150‑1 (MQ=255)
                                                                                                                   ggTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCAt                                  >  2:1689055/1‑150 (MQ=255)
                                                                                                                    gTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATc                                 <  1:744665/150‑1 (MQ=255)
                                                                                                                    gTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATc                                 >  2:4063144/1‑150 (MQ=255)
                                                                                                                     tCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCt                                <  1:2371286/150‑1 (MQ=255)
                                                                                                                      cacaTAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTg                               <  2:1102555/150‑1 (MQ=255)
                                                                                                                       acaTAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGt                              >  1:1847203/1‑150 (MQ=255)
                                                                                                                        caTAGAAGCGTTAATTAACATATGGGAAAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGtt                             <  1:3234235/150‑1 (MQ=255)
                                                                                                                         aTAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTc                            <  2:1726742/150‑1 (MQ=255)
                                                                                                                         aTAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTc                            >  1:3957786/1‑150 (MQ=255)
                                                                                                                          tAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTcg                           >  1:1721207/1‑150 (MQ=255)
                                                                                                                             aaGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCgg                        >  1:1606020/1‑150 (MQ=255)
                                                                                                                             aaGCGTTAATTAACATATGGGAAAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCgg                        >  1:4125097/1‑150 (MQ=255)
                                                                                                                             aaGCGTTAATTAACATATGGGAAAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCgg                        >  1:4125291/1‑150 (MQ=255)
                                                                                                                              aGCGTTAATTAACATATGGGAAAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCggg                       >  1:1375642/1‑150 (MQ=255)
                                                                                                                                cGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTg                     >  1:2179091/1‑150 (MQ=255)
                                                                                                                                cGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTg                     <  1:2028400/150‑1 (MQ=255)
                                                                                                                                 gTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGa                    >  2:624622/1‑150 (MQ=255)
                                                                                                                                 gTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGa                    >  1:2694808/1‑150 (MQ=255)
                                                                                                                                  ttaattaaCATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAg                   <  1:4394388/150‑1 (MQ=255)
                                                                                                                                   taattaaCATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAgg                  <  2:3877932/150‑1 (MQ=255)
                                                                                                                                   taattaaCATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAgg                  >  2:2202705/1‑150 (MQ=255)
                                                                                                                                   taattaaCATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAgg                  >  2:2201950/1‑150 (MQ=255)
                                                                                                                                    aattaaCATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGa                 >  1:2924566/1‑150 (MQ=255)
                                                                                                                                       taaCATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTa              >  1:1390744/1‑150 (MQ=255)
                                                                                                                                          cATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTAttt           <  1:2622114/150‑1 (MQ=255)
                                                                                                                                           atatGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTAtttt          >  1:188113/1‑150 (MQ=255)
                                                                                                                                           atatGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTAtttt          <  1:3066416/150‑1 (MQ=255)
                                                                                                                                            tatGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTTtc         >  1:2641194/1‑150 (MQ=255)
                                                                                                                                             atGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTTtct        <  2:3941974/150‑1 (MQ=255)
                                                                                                                                             atGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTTtct        <  2:3292705/150‑1 (MQ=255)
                                                                                                                                              tGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTTtctc       <  2:2923822/150‑1 (MQ=255)
                                                                                                                                                  aaaaTGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTTTCTCAGTa   >  2:817290/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
TGCTGCACGCAATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGAAAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTTTCTCAGTAT  >  CP009273/2195302‑2195598

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATGCTGCACGCAATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGAAAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTTTCTCAGTAT  >  CP009273/2195301‑2195598
                                                                                                                                                    |                                                                                                                                                     
ATGCTGCACGCAATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATA                                                                                                                                                      <  SRR19973080.2131713/150‑1 (MQ=60)
ATGCTGCACGCAATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATA                                                                                                                                                      <  SRR19973080.2692155/150‑1 (MQ=60)
ATGCTGCACGCAATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATA                                                                                                                                                      <  SRR19973080.3535857/150‑1 (MQ=60)
ATGCTGCACGCAATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATA                                                                                                                                                      <  SRR19973080.4009284/150‑1 (MQ=60)
  GCTGCACGCAATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAAT                                                                                                                                                    <  SRR19973080.2982357/150‑1 (MQ=60)
      CACGCAATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGAAAATGACA                                                                                                                                                <  SRR19973080.121999/150‑1 (MQ=60)
       ACGCAATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAA                                                                                                                                               >  SRR19973080.4049588/1‑150 (MQ=60)
                ACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGAAAATGACAACTACTCACA                                                                                                                                      <  SRR19973080.4394816/150‑1 (MQ=60)
                ACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGAAAATGACAACTACTCACA                                                                                                                                      >  SRR19973080.1288243/1‑150 (MQ=60)
                   CTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTA                                                                                                                                   <  SRR19973080.1933415/150‑1 (MQ=60)
                    TTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTATTTAACATATGGGATAATGACAACTACTCACATTAT                                                                                                                                  <  SRR19973080.1010261/150‑1 (MQ=60)
                    TTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTAT                                                                                                                                  <  SRR19973080.1457298/150‑1 (MQ=60)
                    TTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTAT                                                                                                                                  <  SRR19973080.1457273/150‑1 (MQ=60)
                         TCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCC                                                                                                                             <  SRR19973080.569409/150‑1 (MQ=60)
                          CAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCG                                                                                                                            <  SRR19973080.1607192/150‑1 (MQ=60)
                          CAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCG                                                                                                                            >  SRR19973080.3240384/1‑150 (MQ=60)
                                 ATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGA                                                                                                                     <  SRR19973080.4077856/150‑1 (MQ=60)
                                  TTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAG                                                                                                                    <  SRR19973080.4489623/150‑1 (MQ=60)
                                    ACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGATGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTG                                                                                                                  <  SRR19973080.578926/150‑1 (MQ=60)
                                    ACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTG                                                                                                                  >  SRR19973080.4118927/1‑150 (MQ=60)
                                    ACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTG                                                                                                                  >  SRR19973080.1945079/1‑150 (MQ=60)
                                    ACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTG                                                                                                                  <  SRR19973080.578691/150‑1 (MQ=60)
                                    ACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTG                                                                                                                  >  SRR19973080.1944602/1‑150 (MQ=60)
                                      TACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTC                                                                                                                >  SRR19973080.2771662/1‑150 (MQ=60)
                                        CCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTG                                                                                                              <  SRR19973080.588889/150‑1 (MQ=60)
                                        CCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTG                                                                                                              <  SRR19973080.588860/150‑1 (MQ=60)
                                          AGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGA                                                                                                            <  SRR19973080.1131901/150‑1 (MQ=60)
                                          AGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGAAAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGA                                                                                                            <  SRR19973080.1034717/150‑1 (MQ=60)
                                          AGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTCAATTAACATATGGGAAAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGA                                                                                                            <  SRR19973080.1034743/150‑1 (MQ=60)
                                           GGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAA                                                                                                           >  SRR19973080.4232783/1‑150 (MQ=60)
                                            GGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGAAAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAAC                                                                                                          >  SRR19973080.3731853/1‑150 (MQ=60)
                                                GGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGT                                                                                                      >  SRR19973080.1108893/1‑150 (MQ=60)
                                                   TTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATT                                                                                                   <  SRR19973080.866578/150‑1 (MQ=60)
                                                   TTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATT                                                                                                   <  SRR19973080.866616/150‑1 (MQ=60)
                                                   TTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATT                                                                                                   <  SRR19973080.866656/150‑1 (MQ=60)
                                                        GAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGAAAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTG                                                                                              >  SRR19973080.3695282/1‑150 (MQ=60)
                                                         AGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGAAAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGG                                                                                             <  SRR19973080.3008367/150‑1 (MQ=60)
                                                          GATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGC                                                                                            <  SRR19973080.3790625/150‑1 (MQ=60)
                                                          GATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGC                                                                                            <  SRR19973080.3790601/150‑1 (MQ=60)
                                                            TCCTGAATGGCTGGAACTCCCCGGAAGCACAATTATCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCAC                                                                                          <  SRR19973080.963628/150‑1 (MQ=60)
                                                             CCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACA                                                                                         <  SRR19973080.3249793/150‑1 (MQ=60)
                                                              CTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAG                                                                                        <  SRR19973080.4460075/150‑1 (MQ=60)
                                                               TGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGT                                                                                       >  SRR19973080.3197943/1‑150 (MQ=60)
                                                                GAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTT                                                                                      >  SRR19973080.3307465/1‑150 (MQ=60)
                                                                 AATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTG                                                                                     <  SRR19973080.4080660/150‑1 (MQ=60)
                                                                         GAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGA                                                                             <  SRR19973080.597705/150‑1 (MQ=60)
                                                                         GAACTCCCCGGAAGCACAATGAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGA                                                                             <  SRR19973080.597740/150‑1 (MQ=60)
                                                                          AACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAG                                                                            >  SRR19973080.1780941/1‑150 (MQ=60)
                                                                            CTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGAT                                                                          <  SRR19973080.3134071/150‑1 (MQ=60)
                                                                                 CGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATGTGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTT                                                                     <  SRR19973080.3149252/150‑1 (MQ=60)
                                                                                   GAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTG                                                                   >  SRR19973080.1236479/1‑150 (MQ=60)
                                                                                      GCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCCCATAGAAGCGTTAATTAACATATGGGAAAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACC                                                                >  SRR19973080.3183032/1‑150 (MQ=60)
                                                                                      GCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGAAAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACC                                                                >  SRR19973080.903035/1‑150 (MQ=60)
                                                                                        ACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTT                                                              >  SRR19973080.981729/1‑150 (MQ=60)
                                                                                        ACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTT                                                              <  SRR19973080.2688595/150‑1 (MQ=60)
                                                                                         CAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGAAAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTC                                                             <  SRR19973080.408913/150‑1 (MQ=60)
                                                                                             TAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGC                                                         >  SRR19973080.2084784/1‑150 (MQ=60)
                                                                                              AGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCT                                                        >  SRR19973080.1264509/1‑150 (MQ=60)
                                                                                              AGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCT                                                        <  SRR19973080.3762728/150‑1 (MQ=60)
                                                                                               GCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGAAAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTG                                                       >  SRR19973080.3879944/1‑150 (MQ=60)
                                                                                               GCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGAAAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTG                                                       >  SRR19973080.2008857/1‑150 (MQ=60)
                                                                                                 AGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCG                                                     >  SRR19973080.4521634/1‑150 (MQ=60)
                                                                                                 AGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACAGATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCG                                                     <  SRR19973080.604115/150‑1 (MQ=60)
                                                                                                   AACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTA                                                   <  SRR19973080.1492632/150‑1 (MQ=60)
                                                                                                     CAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATC                                                 <  SRR19973080.1786208/150‑1 (MQ=60)
                                                                                                     CAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATC                                                 <  SRR19973080.2034715/150‑1 (MQ=60)
                                                                                                     CAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGAAAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATC                                                 <  SRR19973080.3350606/150‑1 (MQ=60)
                                                                                                           CTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAG                                           <  SRR19973080.1669199/150‑1 (MQ=60)
                                                                                                             CCGCAGCGGTCACATAGAATCTTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAA                                         <  SRR19973080.4463785/150‑1 (MQ=60)
                                                                                                                CAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAA                                      >  SRR19973080.4500238/1‑150 (MQ=60)
                                                                                                                 AGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAG                                     >  SRR19973080.3380510/1‑150 (MQ=60)
                                                                                                                 AGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAG                                     >  SRR19973080.2933145/1‑150 (MQ=60)
                                                                                                                 AGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAG                                     >  SRR19973080.2933173/1‑150 (MQ=60)
                                                                                                                     GTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATC                                 <  SRR19973080.748890/150‑1 (MQ=60)
                                                                                                                     GTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATC                                 <  SRR19973080.3021982/150‑1 (MQ=60)
                                                                                                                      TCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCT                                <  SRR19973080.2384474/150‑1 (MQ=60)
                                                                                                                      TCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCT                                <  SRR19973080.2384424/150‑1 (MQ=60)
                                                                                                                      TCACATAGAAGCGTTAATTAACATATGGGAAAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCT                                <  SRR19973080.3294979/150‑1 (MQ=60)
                                                                                                                        ACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGT                              >  SRR19973080.1857588/1‑150 (MQ=60)
                                                                                                                         CATAGAAGCGTTAATTAACATATGGGAAAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTT                             <  SRR19973080.3252055/150‑1 (MQ=60)
                                                                                                                          ATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTC                            >  SRR19973080.3979585/1‑150 (MQ=60)
                                                                                                                          ATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTC                            >  SRR19973080.38060/1‑150 (MQ=60)
                                                                                                                           TAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCG                           >  SRR19973080.1731173/1‑150 (MQ=60)
                                                                                                                              AAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGG                        >  SRR19973080.1615419/1‑150 (MQ=60)
                                                                                                                              AAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGG                        >  SRR19973080.1841652/1‑150 (MQ=60)
                                                                                                                              AAGCGTTAATTAACATATGGGAAAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGG                        >  SRR19973080.4147458/1‑150 (MQ=60)
                                                                                                                              AAGCGTTAATTAACATATGGGAAAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGG                        >  SRR19973080.4147513/1‑150 (MQ=60)
                                                                                                                              AAGCGTTAATTAACATATGGGAAAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGG                        >  SRR19973080.4147652/1‑150 (MQ=60)
                                                                                                                               AGCGTTAATTAACATATGGGAAAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGG                       >  SRR19973080.1383861/1‑150 (MQ=60)
                                                                                                                               AGCGTTAATTAACATATGGGAAAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGG                       >  SRR19973080.1383960/1‑150 (MQ=60)
                                                                                                                               AGCGTTAATTAACATATGGGAAAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGG                       >  SRR19973080.1383854/1‑150 (MQ=60)
                                                                                                                               AGCGTTAATTAACATATGGGAAAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGG                       >  SRR19973080.2645784/1‑150 (MQ=60)
                                                                                                                                 CGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTG                     >  SRR19973080.4478059/1‑150 (MQ=60)
                                                                                                                                 CGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTG                     <  SRR19973080.2039923/150‑1 (MQ=60)
                                                                                                                                 CGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTG                     >  SRR19973080.2191225/1‑150 (MQ=60)
                                                                                                                                 CGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTG                     <  SRR19973080.2039895/150‑1 (MQ=60)
                                                                                                                                  GTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGA                    >  SRR19973080.2709547/1‑150 (MQ=60)
                                                                                                                                   TTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAG                   <  SRR19973080.4418315/150‑1 (MQ=60)
                                                                                                                                     AATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGA                 >  SRR19973080.2940484/1‑150 (MQ=60)
                                                                                                                                      ATTAACATATGGGAAAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGAC                <  SRR19973080.2599782/150‑1 (MQ=60)
                                                                                                                                        TAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTA              >  SRR19973080.1399015/1‑150 (MQ=60)
                                                                                                                                           CATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTT           <  SRR19973080.2636318/150‑1 (MQ=60)
                                                                                                                                            ATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTTT          <  SRR19973080.3083106/150‑1 (MQ=60)
                                                                                                                                            ATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTTT          >  SRR19973080.189394/1‑150 (MQ=60)
                                                                                                                                            ATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTTT          >  SRR19973080.3656357/1‑150 (MQ=60)
                                                                                                                                            ATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTTT          >  SRR19973080.189549/1‑150 (MQ=60)
                                                                                                                                             TATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTTTC         >  SRR19973080.2655446/1‑150 (MQ=60)
                                                                                                                                             TATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTTTC         >  SRR19973080.2654788/1‑150 (MQ=60)
                                                                                                                                                    TAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTTTCTCAGTAT  <  SRR19973080.2793355/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
ATGCTGCACGCAATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGAAAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTTTCTCAGTAT  >  CP009273/2195301‑2195598

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑