Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,195,449 A→T 100% E564D (GAA→GAT)  yehI → DUF4132 domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,195,4490AT100.0% 575.7 / NA 172E564D (GAA→GAT) yehIDUF4132 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (92/80);  total (92/80)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 173 total aligned reads displayed.

CTGCACGCAATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGAAAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTTTCTC  >  CP009273/2195304‑2195593
                                                                                                                                                 |                                                                                                                                                
  gcACGCAATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATg                                                                                                                                              >  2:1689119/1‑148 (MQ=255)
   cACGCAATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGa                                                                                                                                             >  2:2758405/1‑148 (MQ=255)
      gCAATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACaa                                                                                                                                          <  1:2448637/148‑1 (MQ=255)
      gCAATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACaa                                                                                                                                          <  2:2910742/148‑1 (MQ=255)
       cAATTCACTCTTTGTTCAGTTGCATTACTACCAGGGGAGTGTTGAAGTGATCCTGAATGGCTTGAACTCCCCGGAAGCACAATTATCAGAACAGGCGCTCCTCATCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAac                                                                                                                                         <  1:2601360/148‑1 (MQ=255)
       cAATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAac                                                                                                                                         <  1:2601362/148‑1 (MQ=255)
        aaTTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAact                                                                                                                                        >  1:3313441/1‑148 (MQ=255)
        aaTTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAact                                                                                                                                        <  1:2545679/148‑1 (MQ=255)
         aTTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAacta                                                                                                                                       <  2:1138461/148‑1 (MQ=255)
         aTTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAacta                                                                                                                                       <  2:2793787/148‑1 (MQ=255)
          ttCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAactac                                                                                                                                      >  2:2027855/1‑148 (MQ=255)
          ttCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAactac                                                                                                                                      >  2:70376/1‑148 (MQ=255)
           tCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAactact                                                                                                                                     <  2:2498793/148‑1 (MQ=255)
           tCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAactact                                                                                                                                     >  2:2528734/1‑148 (MQ=255)
            cACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTc                                                                                                                                    >  1:578409/1‑148 (MQ=255)
              ctctTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTcac                                                                                                                                  >  1:318134/1‑148 (MQ=255)
               tctTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTcaca                                                                                                                                 >  1:1867827/1‑148 (MQ=255)
               tctTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTcaca                                                                                                                                 >  1:3154727/1‑148 (MQ=255)
               tctTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTcaca                                                                                                                                 >  1:2194905/1‑148 (MQ=255)
                  ttGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGTAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTa                                                                                                                              <  2:1357085/148‑1 (MQ=255)
                     ttCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCg                                                                                                                           >  2:2035735/1‑148 (MQ=255)
                      tCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGt                                                                                                                          <  2:3063748/148‑1 (MQ=255)
                       cAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTc                                                                                                                         <  1:1095672/148‑1 (MQ=255)
                       cAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTc                                                                                                                         <  1:2074579/148‑1 (MQ=255)
                            gCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGaa                                                                                                                    >  1:217012/1‑148 (MQ=255)
                             cATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGaaa                                                                                                                   <  2:2952472/148‑1 (MQ=255)
                             cATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGaaa                                                                                                                   >  2:2944183/1‑148 (MQ=255)
                              aTTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGaaaa                                                                                                                  >  2:360909/1‑148 (MQ=255)
                                tactacCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCTCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATAATCGTCCGGAAAaga                                                                                                                >  2:1908495/1‑148 (MQ=255)
                                tactacCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAaga                                                                                                                >  2:3236672/1‑148 (MQ=255)
                                 actacCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTGCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAagag                                                                                                               >  1:1954210/1‑148 (MQ=255)
                                   tacCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAgtg                                                                                                             <  2:918456/148‑1 (MQ=255)
                                   tacCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAgtg                                                                                                             <  2:566412/148‑1 (MQ=255)
                                       aGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTg                                                                                                         >  1:438212/1‑148 (MQ=255)
                                       aGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTg                                                                                                         >  1:439033/1‑148 (MQ=255)
                                        ggggCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTgg                                                                                                        >  2:3064121/1‑148 (MQ=255)
                                         gggCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGa                                                                                                       <  2:932464/148‑1 (MQ=255)
                                         gggCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGa                                                                                                       >  2:2930892/1‑148 (MQ=255)
                                               gTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGt                                                                                                 <  1:3279001/148‑1 (MQ=255)
                                                ttGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGta                                                                                                <  1:2027855/148‑1 (MQ=255)
                                                ttGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGta                                                                                                >  2:177989/1‑148 (MQ=255)
                                                ttGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGta                                                                                                <  2:2016763/148‑1 (MQ=255)
                                                 tGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGtat                                                                                               <  2:1619409/148‑1 (MQ=255)
                                                 tGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGtat                                                                                               >  1:1979123/1‑148 (MQ=255)
                                                 tGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGtat                                                                                               <  1:3064121/148‑1 (MQ=255)
                                                   aaGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGtattt                                                                                             >  1:2812652/1‑148 (MQ=255)
                                                     gagaTCCTGAATGGCTGGAACTCCCCGGAAGCACCATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGACCCTGtatttat                                                                                           >  1:1696340/1‑148 (MQ=255)
                                                     gagaTCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGtatttat                                                                                           >  1:1028238/1‑148 (MQ=255)
                                                     gagaTCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGtatttat                                                                                           <  1:1340193/148‑1 (MQ=255)
                                                     gagaTCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGtatttat                                                                                           <  1:1340212/148‑1 (MQ=255)
                                                      agaTCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGtatttatt                                                                                          <  1:905543/148‑1 (MQ=255)
                                                      agaTCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGtatttatt                                                                                          >  2:1953197/1‑148 (MQ=255)
                                                       gaTCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTg                                                                                         <  2:1234867/148‑1 (MQ=255)
                                                             taaTGGCTGGAACTCACCGGAAGCACAATTAGCAGATCAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACTTATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAg                                                                                   <  1:205432/147‑1 (MQ=255)
                                                               aTGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACAGAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGtt                                                                                 >  2:164210/1‑148 (MQ=255)
                                                                tGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTg                                                                                >  2:144840/1‑148 (MQ=255)
                                                                tGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTg                                                                                <  1:1633044/148‑1 (MQ=255)
                                                                  gCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGcc                                                                              >  2:1303684/1‑148 (MQ=255)
                                                                  gCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGcc                                                                              >  2:2952147/1‑148 (MQ=255)
                                                                     ggAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCcgcg                                                                           <  1:2312402/148‑1 (MQ=255)
                                                                     ggAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCcgcg                                                                           >  2:3026758/1‑148 (MQ=255)
                                                                      gAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGt                                                                          >  1:2413404/1‑148 (MQ=255)
                                                                      gAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGt                                                                          >  2:555008/1‑148 (MQ=255)
                                                                           ccccGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGAt                                                                     <  1:2787045/148‑1 (MQ=255)
                                                                             ccGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATgg                                                                   <  1:856182/148‑1 (MQ=255)
                                                                             ccGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATgg                                                                   <  1:2944183/148‑1 (MQ=255)
                                                                                   gCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGa                                                             >  1:1826112/1‑148 (MQ=255)
                                                                                        aTTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTc                                                        >  2:1737807/1‑148 (MQ=255)
                                                                                         ttAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCt                                                       >  1:1634293/1‑148 (MQ=255)
                                                                                         ttAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCt                                                       >  1:1634209/1‑148 (MQ=255)
                                                                                         ttAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATAGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCt                                                       >  2:1324041/1‑148 (MQ=255)
                                                                                            gCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGc                                                    >  1:2256967/1‑148 (MQ=255)
                                                                                             cAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCt                                                   >  1:611145/1‑148 (MQ=255)
                                                                                                aaCAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCg                                                >  1:199670/1‑148 (MQ=255)
                                                                                                 aCAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGt                                               >  1:2916904/1‑148 (MQ=255)
                                                                                                    ggCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATc                                            >  2:1505474/1‑148 (MQ=255)
                                                                                                     gcgcTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCa                                           <  1:713226/148‑1 (MQ=255)
                                                                                                        cTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATg                                        >  2:436134/1‑148 (MQ=255)
                                                                                                        cTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATg                                        >  2:435934/1‑148 (MQ=255)
                                                                                                        cTCCGCAGCGGTCACATAGAAGCGTTAATGAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATg                                        <  2:2529489/148‑1 (MQ=255)
                                                                                                          ccGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATgag                                      >  1:629128/1‑148 (MQ=255)
                                                                                                               gCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGaaaaa                                 <  1:144840/148‑1 (MQ=255)
                                                                                                                cGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAg                                >  2:2873853/1‑148 (MQ=255)
                                                                                                                  gTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCa                              <  2:668117/148‑1 (MQ=255)
                                                                                                                    cacaTAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATc                            <  1:987815/148‑1 (MQ=255)
                                                                                                                     acaTAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCt                           <  1:1007635/148‑1 (MQ=255)
                                                                                                                      caTAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTg                          <  2:1459815/148‑1 (MQ=255)
                                                                                                                        tAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGtt                        >  1:54820/1‑148 (MQ=255)
                                                                                                                        tAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGtt                        <  2:118008/148‑1 (MQ=255)
                                                                                                                          gAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTcg                      <  2:1524107/148‑1 (MQ=255)
                                                                                                                            aGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTcgcg                    <  2:2916904/148‑1 (MQ=255)
                                                                                                                            aGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTcgcg                    >  2:2719953/1‑148 (MQ=255)
                                                                                                                              cGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCggg                  >  2:2454822/1‑148 (MQ=255)
                                                                                                                                 taattaaCATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGa               <  2:2286279/148‑1 (MQ=255)
                                                                                                                                 taattaaCATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGa               <  2:1954210/148‑1 (MQ=255)
                                                                                                                                   attaaCATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAgg             <  1:2758405/148‑1 (MQ=255)
                                                                                                                                          tatGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTAttt      >  2:1718663/1‑148 (MQ=255)
                                                                                                                                           atGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTAtttt     <  2:1396939/148‑1 (MQ=255)
                                                                                                                                             gggATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTTtct   >  2:1278141/1‑148 (MQ=255)
                                                                                                                                              ggATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTTtctc  >  2:2046555/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CTGCACGCAATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGAAAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTTTCTC  >  CP009273/2195304‑2195593

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GATGCTGCACGCAATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGAAAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTTTCTCAGT  >  CP009273/2195300‑2195596
                                                                                                                                                     |                                                                                                                                                   
GATGCTGCACGCAATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGAT                                                                                                                                                     >  SRR19973070.2107884/1‑150 (MQ=60)
GATGCTGCACGCAATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGAT                                                                                                                                                     <  SRR19973070.2975548/150‑1 (MQ=60)
   GCTGCACGCAATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAAT                                                                                                                                                  <  SRR19973070.1313514/150‑1 (MQ=60)
    CTGCACGCAATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATG                                                                                                                                                 >  SRR19973070.2301487/1‑150 (MQ=60)
    CTGCACGCAATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATG                                                                                                                                                 <  SRR19973070.2709635/150‑1 (MQ=60)
        ACGCAATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAA                                                                                                                                             <  SRR19973070.2349601/150‑1 (MQ=60)
          GCAATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACT                                                                                                                                           >  SRR19973070.3334627/1‑150 (MQ=60)
          GCAATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACT                                                                                                                                           <  SRR19973070.2464656/150‑1 (MQ=60)
           CAATTCACTCTTTGTTCAGTTGCATTACTACCAGGGGAGTGTTGAAGTGATCCTGAATGGCTTGAACTCCCCGGAAGCACAATTATCAGAACAGGCGCTCCTCATCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTA                                                                                                                                          <  SRR19973070.2618344/150‑1 (MQ=60)
           CAATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTA                                                                                                                                          <  SRR19973070.2618346/150‑1 (MQ=60)
           CAATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTA                                                                                                                                          <  SRR19973070.2617655/150‑1 (MQ=60)
            AATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTAC                                                                                                                                         <  SRR19973070.2562321/150‑1 (MQ=60)
             ATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACT                                                                                                                                        <  SRR19973070.1800449/150‑1 (MQ=60)
             ATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACT                                                                                                                                        >  SRR19973070.1076958/1‑150 (MQ=60)
              TTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTC                                                                                                                                       >  SRR19973070.582385/1‑150 (MQ=60)
                CACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCAC                                                                                                                                     >  SRR19973070.320443/1‑150 (MQ=60)
                 ACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACA                                                                                                                                    >  SRR19973070.3175114/1‑150 (MQ=60)
                 ACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACA                                                                                                                                    >  SRR19973070.1880580/1‑150 (MQ=60)
                 ACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACA                                                                                                                                    >  SRR19973070.2209395/1‑150 (MQ=60)
                           CAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCG                                                                                                                          <  SRR19973070.1674389/150‑1 (MQ=60)
                           CAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCG                                                                                                                          <  SRR19973070.1103226/150‑1 (MQ=60)
                           CAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCG                                                                                                                          <  SRR19973070.2088541/150‑1 (MQ=60)
                             GTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGA                                                                                                                        >  SRR19973070.328118/1‑150 (MQ=60)
                              TGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAA                                                                                                                       >  SRR19973070.218592/1‑150 (MQ=60)
                                 CATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAGG                                                                                                                    >  SRR19973070.3172497/1‑150 (MQ=60)
                                   TTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTGCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAG                                                                                                                  >  SRR19973070.1967356/1‑150 (MQ=60)
                                         CCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTG                                                                                                            >  SRR19973070.441391/1‑150 (MQ=60)
                                         CCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTG                                                                                                            <  SRR19973070.967100/150‑1 (MQ=60)
                                         CCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTG                                                                                                            >  SRR19973070.442220/1‑150 (MQ=60)
                                                   GTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTAT                                                                                                  >  SRR19973070.1992505/1‑150 (MQ=60)
                                                   GTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTAT                                                                                                  <  SRR19973070.3300069/150‑1 (MQ=60)
                                                    TTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATT                                                                                                 <  SRR19973070.2041566/150‑1 (MQ=60)
                                                     TGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTT                                                                                                >  SRR19973070.2830841/1‑150 (MQ=60)
                                                     TGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTT                                                                                                <  SRR19973070.3083748/150‑1 (MQ=60)
                                                     TGAAGAGATCATGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCTGTCACATATAAGCGTTAATAAACATAAGGGATAATGACAACAACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACATGTATTT                                                                                                >  SRR19973070.2829972/1‑150 (MQ=60)
                                                       AAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACCATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGACCCTGTATTTAT                                                                                              >  SRR19973070.1708017/1‑150 (MQ=60)
                                                       AAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTAT                                                                                              <  SRR19973070.620301/150‑1 (MQ=60)
                                                       AAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTAT                                                                                              >  SRR19973070.1035523/1‑150 (MQ=60)
                                                        AGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATT                                                                                             <  SRR19973070.563886/150‑1 (MQ=60)
                                                         GAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTG                                                                                            <  SRR19973070.1349402/150‑1 (MQ=60)
                                                         GAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTG                                                                                            <  SRR19973070.1349421/150‑1 (MQ=60)
                                                          AGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGG                                                                                           <  SRR19973070.911972/150‑1 (MQ=60)
                                                           GATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGC                                                                                          >  SRR19973070.1121336/1‑150 (MQ=60)
                                                                 TAATGGCTGGAACTCACCGGAAGCACAATTAGCAGATCAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACTTATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTT                                                                                    <  SRR19973070.206972/150‑1 (MQ=60)
                                                                 GAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTT                                                                                    <  SRR19973070.207249/150‑1 (MQ=60)
                                                                    TGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCC                                                                                 <  SRR19973070.1644961/150‑1 (MQ=60)
                                                                    TGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCC                                                                                 <  SRR19973070.1644370/150‑1 (MQ=60)
                                                                     GGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCG                                                                                <  SRR19973070.136668/150‑1 (MQ=60)
                                                                        TGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGT                                                                             >  SRR19973070.2429299/1‑150 (MQ=60)
                                                                         GGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTG                                                                            <  SRR19973070.2327773/150‑1 (MQ=60)
                                                                               CCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGCAGAGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGG                                                                      <  SRR19973070.2804984/150‑1 (MQ=60)
                                                                               CCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGG                                                                      <  SRR19973070.2804765/150‑1 (MQ=60)
                                                                                 CCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCT                                                                    <  SRR19973070.2963148/150‑1 (MQ=60)
                                                                                 CCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCT                                                                    <  SRR19973070.862092/150‑1 (MQ=60)
                                                                                     AAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGA                                                                >  SRR19973070.1838671/1‑150 (MQ=60)
                                                                                      AGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGAC                                                               >  SRR19973070.447304/1‑150 (MQ=60)
                                                                                       GCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACC                                                              <  SRR19973070.706855/150‑1 (MQ=60)
                                                                                          CAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTC                                                           >  SRR19973070.198927/1‑150 (MQ=60)
                                                                                           AATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCT                                                          >  SRR19973070.1645626/1‑150 (MQ=60)
                                                                                           AATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCT                                                          >  SRR19973070.1645542/1‑150 (MQ=60)
                                                                                              TAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGC                                                       >  SRR19973070.2271937/1‑150 (MQ=60)
                                                                                               AGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCT                                                      >  SRR19973070.615431/1‑150 (MQ=60)
                                                                                                  AGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCG                                                   >  SRR19973070.201188/1‑150 (MQ=60)
                                                                                                   GAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGT                                                  >  SRR19973070.2937994/1‑150 (MQ=60)
                                                                                                   GAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGT                                                  >  SRR19973070.2935750/1‑150 (MQ=60)
                                                                                                   GAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGT                                                  >  SRR19973070.396749/1‑150 (MQ=60)
                                                                                                       AGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCA                                              >  SRR19973070.2045732/1‑150 (MQ=60)
                                                                                                         GCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAAT                                            <  SRR19973070.718317/150‑1 (MQ=60)
                                                                                                            CTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAG                                         >  SRR19973070.633588/1‑150 (MQ=60)
                                                                                                                 CAGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGATCTTCTGGCTGCGTATCAATGAGAAAAA                                    <  SRR19973070.1996447/150‑1 (MQ=60)
                                                                                                                  AGCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAG                                   >  SRR19973070.2153148/1‑150 (MQ=60)
                                                                                                                   GCGGTCACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGC                                  <  SRR19973070.146079/150‑1 (MQ=60)
                                                                                                                        CACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTG                             <  SRR19973070.994854/150‑1 (MQ=60)
                                                                                                                         ACATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGT                            <  SRR19973070.1014809/150‑1 (MQ=60)
                                                                                                                          CATAGAAGCGTTAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTT                           >  SRR19973070.55464/1‑150 (MQ=60)
                                                                                                                                     TAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGG                <  SRR19973070.3046034/150‑1 (MQ=60)
                                                                                                                                     TAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGG                <  SRR19973070.2225018/150‑1 (MQ=60)
                                                                                                                                     TAATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGG                <  SRR19973070.1870695/150‑1 (MQ=60)
                                                                                                                                      AATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGA               >  SRR19973070.3329243/1‑150 (MQ=60)
                                                                                                                                       ATTAACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGAC              <  SRR19973070.2776103/150‑1 (MQ=60)
                                                                                                                                           ACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATT          >  SRR19973070.2515956/1‑150 (MQ=60)
                                                                                                                                           ACATATGGGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATT          >  SRR19973070.792441/1‑150 (MQ=60)
                                                                                                                                                  GGATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTTTCTCAG   <  SRR19973070.1625462/150‑1 (MQ=60)
                                                                                                                                                   GATAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTTTCTCAGT  >  SRR19973070.861622/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                   
GATGCTGCACGCAATTCACTCTTTGTTCAGTGGCATTACTACCAGGGGCGGGTTGAAGAGATCCTGAATGGCTGGAACTCCCCGGAAGCACAATTAGCAGAACAGGCGCTCCGCAGCGGTCACATAGAAGCGTTAATTAACATATGGGAAAATGACAACTACTCACATTATCGTCCGGAAAAGAGTGTCTGGAACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTTTCTCAGT  >  CP009273/2195300‑2195596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑