Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,237,098 G→T 100% H12N (CAT→AAT)  folE ← GTP cyclohydrolase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,237,0980GT98.0% 841.3 / 7.3 248H12N (CAT→AAT) folEGTP cyclohydrolase I
Reads supporting (aligned to +/- strand):  ref base G (1/4);  new base T (118/125);  total (119/129)
Fisher's exact test for biased strand distribution p-value = 3.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 248 total aligned reads displayed.

TCAAACTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCAC  >  CP009273/2236951‑2237244
                                                                                                                                                   |                                                                                                                                                  
tCAAACTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTaa                                                                                                                                                  >  1:1853207/1‑150 (MQ=255)
  aaaCTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAAcc                                                                                                                                                >  2:4046065/1‑150 (MQ=255)
    aCTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAg                                                                                                                                              >  2:869069/1‑150 (MQ=255)
     cTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAgg                                                                                                                                             >  2:3689912/1‑150 (MQ=255)
      tGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAggg                                                                                                                                            >  1:3701918/1‑150 (MQ=255)
        tcatcaGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGcc                                                                                                                                          <  2:520352/150‑1 (MQ=255)
         catcaGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCg                                                                                                                                         <  1:2141841/150‑1 (MQ=255)
              gCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGcttct                                                                                                                                    <  1:2967212/150‑1 (MQ=255)
               ccAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGcttctt                                                                                                                                   >  1:3187137/1‑150 (MQ=255)
                 aGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTa                                                                                                                                 >  2:678678/1‑150 (MQ=255)
                 aGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTa                                                                                                                                 <  1:2532287/150‑1 (MQ=255)
                  ggTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGAAATAAGGCTTTTGCGCGTTTCGTTATCCAATTCATGCACGGGCGGGCGCAGCGGTGATACCAGTACTCGCGCAACAAACGCTTCATTAACCAGGGCCGcttcataac                                                                                                                                >  1:7318/1‑145 (MQ=255)
                     cGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGa                                                                                                                             >  1:1059326/1‑150 (MQ=255)
                     cGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGa                                                                                                                             <  1:2732556/150‑1 (MQ=255)
                       agaTTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGt                                                                                                                           <  2:3471740/150‑1 (MQ=255)
                       agaTTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGt                                                                                                                           <  2:528478/150‑1 (MQ=255)
                           tCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATg                                                                                                                       <  1:463860/150‑1 (MQ=255)
                             agcagcTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGc                                                                                                                     >  2:2328416/1‑150 (MQ=255)
                                agcTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCAtt                                                                                                                  >  1:3052878/1‑150 (MQ=255)
                                   tGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTAt                                                                                                               <  1:4491195/150‑1 (MQ=255)
                                    gCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATg                                                                                                              <  2:3009680/150‑1 (MQ=255)
                                      aTGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGAt                                                                                                            >  2:802092/1‑150 (MQ=255)
                                        gATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGAttt                                                                                                          >  2:2799268/1‑150 (MQ=255)
                                         aTTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTtc                                                                                                         <  1:1764572/150‑1 (MQ=255)
                                           ttCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCAGTTATGATTtctc                                                                                                       >  1:244958/1‑150 (MQ=255)
                                             cGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCt                                                                                                     <  2:3915136/150‑1 (MQ=255)
                                             cGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCt                                                                                                     <  2:3970210/150‑1 (MQ=255)
                                              ggTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTg                                                                                                    >  2:897433/1‑150 (MQ=255)
                                               gTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGc                                                                                                   >  2:1618862/1‑150 (MQ=255)
                                               gTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGc                                                                                                   >  2:491576/1‑150 (MQ=255)
                                                tCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCa                                                                                                  >  2:2571335/1‑150 (MQ=255)
                                                 cATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAg                                                                                                 >  1:1964131/1‑150 (MQ=255)
                                                     tGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGgtgt                                                                                             <  1:3510739/150‑1 (MQ=255)
                                                      gACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGtgtg                                                                                            <  1:4154268/150‑1 (MQ=255)
                                                        ccAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGAc                                                                                          <  2:1719255/150‑1 (MQ=255)
                                                          aGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGc                                                                                        >  1:3385647/1‑150 (MQ=255)
                                                          aGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGc                                                                                        >  2:4599254/1‑150 (MQ=255)
                                                          aGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGc                                                                                        >  1:2501381/1‑150 (MQ=255)
                                                          aGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCAGTTATGATTTCTCCTGCAGGTGTGACGc                                                                                        >  2:1500453/1‑150 (MQ=255)
                                                              aTAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTcc                                                                                    <  1:4547239/150‑1 (MQ=255)
                                                               tAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCg                                                                                   >  2:3565519/1‑150 (MQ=255)
                                                               tAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCg                                                                                   <  1:1918753/150‑1 (MQ=255)
                                                                aaGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGc                                                                                  >  1:316657/1‑150 (MQ=255)
                                                                   gCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCt                                                                               <  2:1976884/150‑1 (MQ=255)
                                                                   gCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCt                                                                               <  1:1126616/150‑1 (MQ=255)
                                                                     ttttGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGc                                                                             <  1:4143396/150‑1 (MQ=255)
                                                                      tttGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCg                                                                            <  1:718709/150‑1 (MQ=255)
                                                                      tttGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCg                                                                            <  1:1967297/150‑1 (MQ=255)
                                                                       ttGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGt                                                                           <  1:1327125/150‑1 (MQ=255)
                                                                        tGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTg                                                                          <  1:3523604/150‑1 (MQ=255)
                                                                          cgcgTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTggg                                                                        <  1:2056212/150‑1 (MQ=255)
                                                                           gcgTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTgggg                                                                       <  1:3995063/150‑1 (MQ=255)
                                                                              tttCCTTATCCATTTCATGCACGGGCGGGAGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGAGGGGATGTAGTATTTCTCCTGCAGGTGTGACGCCTCCGCCCAGCGTGGGGGCa                                                                    >  1:4469833/1‑150 (MQ=255)
                                                                               ttCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCAGCAGGTGTGACGCCTCCGCCCTGCGAGGGGGCaa                                                                   >  2:3356286/1‑150 (MQ=255)
                                                                                 cGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAg                                                                 <  2:2055445/150‑1 (MQ=255)
                                                                                    tATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGtta                                                              <  2:4446685/150‑1 (MQ=255)
                                                                                    tATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGtta                                                              >  1:4024670/1‑150 (MQ=255)
                                                                                    tATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGtta                                                              <  1:966670/150‑1 (MQ=255)
                                                                                     aTCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGttat                                                             <  2:4222727/150‑1 (MQ=255)
                                                                                         atatCATACACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGATTGATGGCAATTATGATTTCTCCAGCAGGTGTTACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTaat                                                         >  1:4466335/4‑149 (MQ=255)
                                                                                         aTTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAg                                                         <  1:2385344/150‑1 (MQ=255)
                                                                                         aTTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAg                                                         <  1:872878/150‑1 (MQ=255)
                                                                                           ttCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCt                                                       <  1:370303/150‑1 (MQ=255)
                                                                                               tGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGAtt                                                   <  2:3435931/150‑1 (MQ=255)
                                                                                                   cgggcgggCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACa                                               <  2:2892018/150‑1 (MQ=255)
                                                                                                       cgggcgCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCt                                           >  2:2159232/1‑150 (MQ=255)
                                                                                                        gggcgCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGGATTTATGATTTATCCTTCAGGTGTGGCGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTg                                          >  1:1193083/1‑150 (MQ=255)
                                                                                                        gggcgCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCCTTTATGATTTCTACTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTg                                          >  1:1684934/1‑150 (MQ=255)
                                                                                                            gcAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGatta                                      <  1:1453605/150‑1 (MQ=255)
                                                                                                             cAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGattat                                     >  2:29515/1‑150 (MQ=255)
                                                                                                             cAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGattat                                     >  2:29875/1‑150 (MQ=255)
                                                                                                              aGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGattatt                                    <  2:3200371/150‑1 (MQ=255)
                                                                                                              aGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGattatt                                    <  1:2676793/150‑1 (MQ=255)
                                                                                                                cGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCGTTACTGAGTGATGGCATTTATGATTTATCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCAGATTTACAGCCAGATTATtgt                                  >  1:1971094/1‑150 (MQ=255)
                                                                                                                cGGTGTTTCCAGTCCTCGCACAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATtgt                                  <  2:1758871/150‑1 (MQ=255)
                                                                                                                  gtgtTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGc                                >  2:1811755/1‑150 (MQ=255)
                                                                                                                  gtgtTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGc                                <  2:1277516/150‑1 (MQ=255)
                                                                                                                    gtTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGt                              >  1:3477890/1‑150 (MQ=255)
                                                                                                                    gtTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGt                              >  1:2812074/1‑150 (MQ=255)
                                                                                                                    gtTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATGTACAGCCTGATTATTGTGCGt                              >  2:3782272/1‑150 (MQ=255)
                                                                                                                      ttCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGa                            <  1:677527/150‑1 (MQ=255)
                                                                                                                       tCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAg                           <  1:680706/150‑1 (MQ=255)
                                                                                                                            tCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAggcggc                      <  2:499734/150‑1 (MQ=255)
                                                                                                                              cTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGgcgc                    >  2:4017287/1‑150 (MQ=255)
                                                                                                                                  cgcAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACAt                >  2:2821529/1‑150 (MQ=255)
                                                                                                                                    cAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATaa              <  2:110430/150‑1 (MQ=255)
                                                                                                                                    cAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATaa              >  2:1597635/1‑150 (MQ=255)
                                                                                                                                    cAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATaa              <  2:2424270/150‑1 (MQ=255)
                                                                                                                                     aaCTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAAt             <  1:3570146/150‑1 (MQ=255)
                                                                                                                                      aCTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATc            >  2:3159147/1‑150 (MQ=255)
                                                                                                                                         aaCGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAg         >  2:4351245/1‑150 (MQ=255)
                                                                                                                                          aCGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGt        <  2:4139235/150‑1 (MQ=255)
                                                                                                                                           cGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTa       >  2:79307/1‑150 (MQ=255)
                                                                                                                                            gCTTCATTAACCAGGGCCGCTTCGTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTAt      >  1:2224744/1‑150 (MQ=255)
                                                                                                                                             cTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTAtt     <  2:4288513/150‑1 (MQ=255)
                                                                                                                                              ttCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTc    <  1:3345128/150‑1 (MQ=255)
                                                                                                                                              ttCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATGTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTc    <  1:2775358/150‑1 (MQ=255)
                                                                                                                                              ttCATTAACCAGGGCCGCTTCTTTACTGAGTGAAGGCAATTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCAGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTc    >  2:2542554/1‑150 (MQ=255)
                                                                                                                                               tCATTAACCAGGGCAGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTca   <  1:2328522/150‑1 (MQ=255)
                                                                                                                                                cATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTcac  <  1:4136966/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TCAAACTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCAC  >  CP009273/2236951‑2237244

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCAAACTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCAC  >  CP009273/2236951‑2237244
                                                                                                                                                   |                                                                                                                                                  
TCAAACTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAA                                                                                                                                                  >  SRR19973072.1857219/1‑150 (MQ=60)
 CAAACTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAAC                                                                                                                                                 >  SRR19973072.4029060/1‑150 (MQ=60)
   AACTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCA                                                                                                                                               >  SRR19973072.3209889/1‑150 (MQ=60)
    ACTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAG                                                                                                                                              <  SRR19973072.3407850/150‑1 (MQ=60)
      TGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGG                                                                                                                                            >  SRR19973072.3709728/1‑150 (MQ=60)
      TGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGG                                                                                                                                            >  SRR19973072.3709718/1‑150 (MQ=60)
        TCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCC                                                                                                                                          <  SRR19973072.2106300/150‑1 (MQ=60)
        TCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCC                                                                                                                                          <  SRR19973072.2524216/150‑1 (MQ=60)
         CATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCG                                                                                                                                         <  SRR19973072.2146506/150‑1 (MQ=60)
         CATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCG                                                                                                                                         >  SRR19973072.545595/1‑150 (MQ=60)
          ATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGC                                                                                                                                        >  SRR19973072.1280278/1‑150 (MQ=60)
              GCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCT                                                                                                                                    <  SRR19973072.2973547/150‑1 (MQ=60)
               CCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTT                                                                                                                                   >  SRR19973072.3193858/1‑150 (MQ=60)
                 AGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTA                                                                                                                                 >  SRR19973072.3184135/1‑150 (MQ=60)
                 AGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTA                                                                                                                                 <  SRR19973072.2537790/150‑1 (MQ=60)
                  GGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTAC                                                                                                                                >  SRR19973072.7385/1‑150 (MQ=60)
                  GGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGAAATAAGGCTTTTGCGCGTTTCGTTATCCAATTCATGCACGGGCGGGCGCAGCGGTGATACCAGTACTCGCGCAACAAACGCTTCATTAACCAGGGCCGCTTCataac                                                                                                                                >  SRR19973072.7341/1‑145 (MQ=60)
                    TCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTG                                                                                                                              <  SRR19973072.1068994/150‑1 (MQ=60)
                     CGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGA                                                                                                                             >  SRR19973072.1061597/1‑150 (MQ=60)
                     CGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGA                                                                                                                             <  SRR19973072.2738385/150‑1 (MQ=60)
                      GAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAG                                                                                                                            >  SRR19973072.3685691/1‑150 (MQ=60)
                       AGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGT                                                                                                                           >  SRR19973072.2339587/1‑150 (MQ=60)
                         ATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGA                                                                                                                         <  SRR19973072.1705109/150‑1 (MQ=60)
                           TCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATG                                                                                                                       <  SRR19973072.2787001/150‑1 (MQ=60)
                           TCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATG                                                                                                                       <  SRR19973072.464934/150‑1 (MQ=60)
                             AGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGC                                                                                                                     <  SRR19973072.1919665/150‑1 (MQ=60)
                              GCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCA                                                                                                                    <  SRR19973072.2814838/150‑1 (MQ=60)
                                AGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATT                                                                                                                  >  SRR19973072.3059353/1‑150 (MQ=60)
                                AGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATT                                                                                                                  >  SRR19973072.3059332/1‑150 (MQ=60)
                                AGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATT                                                                                                                  >  SRR19973072.2717994/1‑150 (MQ=60)
                                AGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATT                                                                                                                  >  SRR19973072.2717954/1‑150 (MQ=60)
                                 GCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTT                                                                                                                 <  SRR19973072.2234969/150‑1 (MQ=60)
                                   TGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTAT                                                                                                               <  SRR19973072.4500682/150‑1 (MQ=60)
                                      ATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGAT                                                                                                            <  SRR19973072.3433181/150‑1 (MQ=60)
                                         ATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTC                                                                                                         <  SRR19973072.1768428/150‑1 (MQ=60)
                                           TTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCAGTTATGATTTCTC                                                                                                       >  SRR19973072.245548/1‑150 (MQ=60)
                                                TCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCATGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTTCA                                                                                                  >  SRR19973072.2521452/1‑150 (MQ=60)
                                                 CATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAG                                                                                                 >  SRR19973072.1275581/1‑150 (MQ=60)
                                                 CATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTATCCTGCAG                                                                                                 >  SRR19973072.1968330/1‑150 (MQ=60)
                                                   TATGACCAGCAATAAGGCTTTTTCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGT                                                                                               <  SRR19973072.4498442/150‑1 (MQ=60)
                                                     TGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGT                                                                                             <  SRR19973072.1191542/150‑1 (MQ=60)
                                                     TGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGT                                                                                             <  SRR19973072.3518103/150‑1 (MQ=60)
                                                     TGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGT                                                                                             <  SRR19973072.3518175/150‑1 (MQ=60)
                                                      GACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTG                                                                                            <  SRR19973072.248048/150‑1 (MQ=60)
                                                      GACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTG                                                                                            <  SRR19973072.4162637/150‑1 (MQ=60)
                                                      GACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTG                                                                                            <  SRR19973072.4162977/150‑1 (MQ=60)
                                                      GACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTG                                                                                            <  SRR19973072.4162525/150‑1 (MQ=60)
                                                          AGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGC                                                                                        >  SRR19973072.2506816/1‑150 (MQ=60)
                                                          AGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGC                                                                                        <  SRR19973072.690024/150‑1 (MQ=60)
                                                          AGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGC                                                                                        >  SRR19973072.3392516/1‑150 (MQ=60)
                                                          AGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGC                                                                                        >  SRR19973072.3392803/1‑150 (MQ=60)
                                                              ATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCC                                                                                    <  SRR19973072.4556827/150‑1 (MQ=60)
                                                              ATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCC                                                                                    <  SRR19973072.4556816/150‑1 (MQ=60)
                                                               TAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCG                                                                                   <  SRR19973072.1922834/150‑1 (MQ=60)
                                                                AAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGC                                                                                  >  SRR19973072.317378/1‑150 (MQ=60)
                                                                AAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCACCGC                                                                                  >  SRR19973072.787102/1‑150 (MQ=60)
                                                                 AGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCC                                                                                 >  SRR19973072.4147918/1‑150 (MQ=60)
                                                                  GGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTATTTTATTAGTGATGGCAATTTTGATTTCTCCAGCAGGTGTGACGCCTCCGCCC                                                                                >  SRR19973072.2442140/1‑150 (MQ=60)
                                                                   GCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCT                                                                               <  SRR19973072.2421020/150‑1 (MQ=60)
                                                                   GCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCT                                                                               <  SRR19973072.1128981/150‑1 (MQ=60)
                                                                     TTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGC                                                                             <  SRR19973072.4152086/150‑1 (MQ=60)
                                                                     TTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGC                                                                             <  SRR19973072.1816458/150‑1 (MQ=60)
                                                                      TTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCG                                                                            <  SRR19973072.1971500/150‑1 (MQ=60)
                                                                      TTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCG                                                                            <  SRR19973072.1971508/150‑1 (MQ=60)
                                                                      TTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCG                                                                            <  SRR19973072.720041/150‑1 (MQ=60)
                                                                      TTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCG                                                                            <  SRR19973072.720267/150‑1 (MQ=60)
                                                                      TTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCG                                                                            <  SRR19973072.720282/150‑1 (MQ=60)
                                                                       TTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGT                                                                           <  SRR19973072.1330026/150‑1 (MQ=60)
                                                                       TTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGT                                                                           <  SRR19973072.299788/150‑1 (MQ=60)
                                                                        TGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTG                                                                          <  SRR19973072.3531151/150‑1 (MQ=60)
                                                                        TGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTG                                                                          <  SRR19973072.3531005/150‑1 (MQ=60)
                                                                          CGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGG                                                                        <  SRR19973072.2060687/150‑1 (MQ=60)
                                                                          CGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGG                                                                        <  SRR19973072.2062051/150‑1 (MQ=60)
                                                                           GCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGG                                                                       <  SRR19973072.4003425/150‑1 (MQ=60)
                                                                              TTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCAGCGTGGGGGCA                                                                    >  SRR19973072.4479164/1‑150 (MQ=60)
                                                                              TTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCGTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCA                                                                    >  SRR19973072.4479254/1‑150 (MQ=60)
                                                                              TTTCCTTATCCATTTCATGCACGGGCGGGAGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGAGGGGATGTAGTATTTCTCCTGCAGGTGTGACGCCTCCGCCCAGCGTGGGGGCA                                                                    >  SRR19973072.4479264/1‑150 (MQ=60)
                                                                                 CGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAG                                                                 <  SRR19973072.3930447/150‑1 (MQ=60)
                                                                                   TTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTT                                                               >  SRR19973072.2895226/1‑150 (MQ=60)
                                                                                    TATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTA                                                              <  SRR19973072.968782/150‑1 (MQ=60)
                                                                                    TATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTA                                                              >  SRR19973072.4033129/1‑150 (MQ=60)
                                                                                    TATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTA                                                              >  SRR19973072.4033192/1‑150 (MQ=60)
                                                                                     ATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTAT                                                             <  SRR19973072.636541/150‑1 (MQ=60)
                                                                                      TCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATT                                                            <  SRR19973072.1968029/150‑1 (MQ=60)
                                                                                      TCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATT                                                            <  SRR19973072.1967872/150‑1 (MQ=60)
                                                                                       CCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTA                                                           >  SRR19973072.2565932/1‑150 (MQ=60)
                                                                                       CCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTA                                                           >  SRR19973072.201090/1‑150 (MQ=60)
                                                                                         ATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAG                                                         <  SRR19973072.2390498/150‑1 (MQ=60)
                                                                                         ATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAG                                                         >  SRR19973072.3300935/1‑150 (MQ=60)
                                                                                         ATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAG                                                         <  SRR19973072.874748/150‑1 (MQ=60)
                                                                                         ATATCATACACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGATTGATGGCAATTATGATTTCTCCAGCAGGTGTTACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAT                                                         >  SRR19973072.4475759/1‑150 (MQ=60)
                                                                                           TTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCT                                                       <  SRR19973072.3525847/150‑1 (MQ=60)
                                                                                           TTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCT                                                       <  SRR19973072.371111/150‑1 (MQ=60)
                                                                                               TGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATT                                                   <  SRR19973072.2751961/150‑1 (MQ=60)
                                                                                                GCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTT                                                  <  SRR19973072.2998911/150‑1 (MQ=60)
                                                                                                 CACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTA                                                 <  SRR19973072.2195578/150‑1 (MQ=60)
                                                                                                  ACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTAC                                                >  SRR19973072.1509422/1‑150 (MQ=60)
                                                                                                        GGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGGATTTATGATTTATCCTTCAGGTGTGGCGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTG                                          >  SRR19973072.1195609/1‑150 (MQ=60)
                                                                                                        GGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCCTTTATGATTTCTACTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTG                                          >  SRR19973072.1688620/1‑150 (MQ=60)
                                                                                                            GCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTA                                      <  SRR19973072.1456730/150‑1 (MQ=60)
                                                                                                             CAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCACCGCCCAGCGTGGGGGCAAAGTTATTAATCTGATTTACAGCCTGATTAT                                     >  SRR19973072.883564/1‑150 (MQ=60)
                                                                                                              AGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATT                                    >  SRR19973072.1798306/1‑150 (MQ=60)
                                                                                                              AGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATT                                    <  SRR19973072.2682504/150‑1 (MQ=60)
                                                                                                                CGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTGGTTACTGAGTGATGGCATTTATGATTTATGCTTCAGGTGTGACGCCACCGCCCTGCGTGGGGGCAAATTTATTAACCTGATTTACAGCCTGATTAattt                                  >  SRR19973072.1975284/1‑146 (MQ=60)
                                                                                                                CGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTTATGGGATTTATGATTTCTACGGCAGGTGTGACGCCGCCGCCCTGCGGGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGT                                  >  SRR19973072.1975305/1‑150 (MQ=60)
                                                                                                                CGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCGTTACTGAGTGATGGCATTTATGATTTATCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCAGATTTACAGCCAGATTATTGT                                  >  SRR19973072.1975315/1‑150 (MQ=60)
                                                                                                                   TGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCG                               <  SRR19973072.2256791/150‑1 (MQ=60)
                                                                                                                   TGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCG                               <  SRR19973072.4596298/150‑1 (MQ=60)
                                                                                                                    GTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGT                              >  SRR19973072.2818099/1‑150 (MQ=60)
                                                                                                                    GTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGT                              >  SRR19973072.3485155/1‑150 (MQ=60)
                                                                                                                      TTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGA                            <  SRR19973072.679034/150‑1 (MQ=60)
                                                                                                                      TTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGA                            <  SRR19973072.679190/150‑1 (MQ=60)
                                                                                                                       TCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAG                           <  SRR19973072.682216/150‑1 (MQ=60)
                                                                                                                           GTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTATCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGG                       >  SRR19973072.3731060/1‑150 (MQ=60)
                                                                                                                                 GCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACA                 <  SRR19973072.1374242/150‑1 (MQ=60)
                                                                                                                                     AACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAAT             <  SRR19973072.3577818/150‑1 (MQ=60)
                                                                                                                                     AACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAAT             <  SRR19973072.3577657/150‑1 (MQ=60)
                                                                                                                                      ACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATC            >  SRR19973072.4254758/1‑150 (MQ=60)
                                                                                                                                       CTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCA           <  SRR19973072.4536221/150‑1 (MQ=60)
                                                                                                                                        TAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGTTGGCATTTATGATTTATCCAGCAGGTGTGACGCCTCCCCCCTGCGGGGGGGCCAAGTTATTAAGCTGATTTAAAGCCTGATTATTGTGCGGGAGGCGGCGCACATAATCCA          >  SRR19973072.3499173/1‑150 (MQ=60)
                                                                                                                                            GCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCAGCGGGGGGGCAAAGGTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTAT      >  SRR19973072.1642163/1‑150 (MQ=60)
                                                                                                                                            GCTTCATTAACCAGGGCCGCTTCGTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTAT      >  SRR19973072.2229566/1‑150 (MQ=60)
                                                                                                                                              TTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTC    <  SRR19973072.3352220/150‑1 (MQ=60)
                                                                                                                                              TTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATGTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTC    <  SRR19973072.2781302/150‑1 (MQ=60)
                                                                                                                                               TCATTAACCAGGGCAGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCA   <  SRR19973072.2333510/150‑1 (MQ=60)
                                                                                                                                                CATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCAC  <  SRR19973072.4145565/150‑1 (MQ=60)
                                                                                                                                                CATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCAC  <  SRR19973072.4145647/150‑1 (MQ=60)
                                                                                                                                                   |                                                                                                                                                  
TCAAACTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCAC  >  CP009273/2236951‑2237244

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑