Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F98 I0 R1 799 294.1 9752082 92.2% 8991419 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,237,098 G→T 62.0% H12N (CAT→AAT)  folE ← GTP cyclohydrolase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,237,0980GT62.0% 115.5 / 268.1 193H12N (CAT→AAT) folEGTP cyclohydrolase I
Reads supporting (aligned to +/- strand):  ref base G (36/37);  new base T (58/62);  total (94/99)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.20e-01

Only 100 of 194 total aligned reads displayed.

CAAACTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGC  >  CP009273/2236952‑2237247
                                                                                                                                                  |                                                                                                                                                     
cAAACTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAAc                                                                                                                                                    >  2:3753743/1‑150 (MQ=255)
 aaaCTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAAcc                                                                                                                                                   <  1:52467/150‑1 (MQ=255)
       tcatcaGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGcc                                                                                                                                             >  2:3106705/1‑150 (MQ=255)
       tcatcaGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGcc                                                                                                                                             <  2:139777/150‑1 (MQ=255)
          tcaGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGct                                                                                                                                          >  2:4486936/1‑150 (MQ=255)
            aGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGcttc                                                                                                                                        <  2:492913/150‑1 (MQ=255)
               cAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCttt                                                                                                                                     <  1:3272771/150‑1 (MQ=255)
               cAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCttt                                                                                                                                     <  1:927855/150‑1 (MQ=255)
               cAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCttt                                                                                                                                     <  2:412457/150‑1 (MQ=255)
               cAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCttt                                                                                                                                     <  1:3627312/150‑1 (MQ=255)
                  gTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACt                                                                                                                                  <  1:107213/150‑1 (MQ=255)
                     gagaTTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAg                                                                                                                               >  2:2997012/1‑150 (MQ=255)
                     gagaTTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAg                                                                                                                               <  1:3465340/150‑1 (MQ=255)
                     gagaTTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTaaagag                                                                                                                               >  2:2018733/1‑145 (MQ=255)
                     gagaTTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTAATGAg                                                                                                                               >  2:2018929/1‑150 (MQ=255)
                      agaTTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTTCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGt                                                                                                                              >  2:82600/1‑150 (MQ=255)
                      agaTTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGt                                                                                                                              <  1:2580062/150‑1 (MQ=255)
                        aTTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGa                                                                                                                            >  1:1287072/1‑150 (MQ=255)
                          tCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCGTGTTTTATGAGTGAtt                                                                                                                          >  1:845855/1‑149 (MQ=255)
                            agcagcTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGc                                                                                                                        <  1:2616484/150‑1 (MQ=255)
                            agcagcTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCGTCTTTACTGTGTGTTggg                                                                                                                        >  1:2022577/1‑149 (MQ=255)
                              cagcTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCAt                                                                                                                      <  1:423831/150‑1 (MQ=255)
                              cagcTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCAt                                                                                                                      <  2:560260/150‑1 (MQ=255)
                               agcTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCagt                                                                                                                     >  2:1118498/1‑148 (MQ=255)
                               agcTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCAtt                                                                                                                     >  2:4648798/1‑150 (MQ=255)
                                 cTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATTGCATTTa                                                                                                                   >  1:623388/1‑150 (MQ=255)
                                 cTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTa                                                                                                                   <  1:3108253/150‑1 (MQ=255)
                                 cTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCGTTACTGAGTGATGGCATTTa                                                                                                                   >  1:3054771/1‑150 (MQ=255)
                                  tGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTAt                                                                                                                  <  2:2452602/150‑1 (MQ=255)
                                   gCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATg                                                                                                                 <  1:246441/150‑1 (MQ=255)
                                        aTTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCGTCTTTACTGAGTGATGGCCTTTATGATTtc                                                                                                            >  2:1216508/1‑150 (MQ=255)
                                         tttCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAGCCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTtct                                                                                                           >  2:1165449/1‑150 (MQ=255)
                                          ttCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTtctc                                                                                                          >  2:4711249/1‑150 (MQ=255)
                                          ttCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTATTTACTGAGTGATTGCATTTATGATTtctc                                                                                                          >  1:3997196/1‑150 (MQ=255)
                                              gTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTGTTTTATGAGTGTTGGCCTTTATGTTTTATCCAGc                                                                                                      >  1:2622395/1‑150 (MQ=255)
                                              gTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTAAAGAGTGATGGCAATTATGATTtctacatc                                                                                                      >  1:2780132/1‑145 (MQ=255)
                                              gTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGc                                                                                                      >  2:2650678/1‑150 (MQ=255)
                                               tCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTATCCTGCa                                                                                                     >  2:4741780/1‑150 (MQ=255)
                                               tCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTACATTAAACAGGGCCGCTTCATTACTGAGTTATTTCATTTATTATTtctaattca                                                                                                     >  2:4741074/1‑144 (MQ=255)
                                                    tGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTTCTTAGTGTTTGGATTTATGATTTCTACTGCAGgtgt                                                                                                >  1:3390474/1‑150 (MQ=255)
                                                    tGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGgtgt                                                                                                <  1:1123688/150‑1 (MQ=255)
                                                     gACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGtgtg                                                                                               <  1:335555/150‑1 (MQ=255)
                                                      aCCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGa                                                                                              >  2:1153283/1‑150 (MQ=255)
                                                      aCCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGa                                                                                              <  2:3662683/150‑1 (MQ=255)
                                                           cAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCt                                                                                         <  1:2089742/150‑1 (MQ=255)
                                                             aTAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTcc                                                                                       <  2:2782360/150‑1 (MQ=255)
                                                             aTAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTGTTTACTGAGTGATGGCAGTTATTATTTCTCCTGCAGGTGTGTCGCCTcc                                                                                       >  1:3630629/1‑150 (MQ=255)
                                                                    ttttGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGGATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCAGc                                                                                >  2:3340669/1‑150 (MQ=255)
                                                                    ttttGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACAGAGTGATGGCATTTATGATTTCTCCTGCAGGGGTGACGCCTCCCCCCAGc                                                                                >  1:4701712/1‑150 (MQ=255)
                                                                     tttGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCg                                                                               <  2:1066516/150‑1 (MQ=255)
                                                                     tttGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCg                                                                               <  1:543583/150‑1 (MQ=255)
                                                                      ttGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGt                                                                              <  2:4512713/150‑1 (MQ=255)
                                                                         cgcgTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTggg                                                                           <  2:1470843/150‑1 (MQ=255)
                                                                              ttCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGTTGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCaa                                                                      >  2:2390668/1‑150 (MQ=255)
                                                                                cGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGAAGCCTCCGCCCAGCGTGGGGGCAAAg                                                                    >  2:4560685/1‑150 (MQ=255)
                                                                                cGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAg                                                                    >  2:4694375/1‑150 (MQ=255)
                                                                                 gTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTGTTTACTGTGTTTTGGGCTTTATGATTTATCCTGCAGGGGTGACGCCACCCCCCTGCGGGGGGGCAAAGt                                                                   >  1:4661290/1‑150 (MQ=255)
                                                                                  ttATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCGTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCACAGtt                                                                  >  2:1117282/1‑150 (MQ=255)
                                                                                   tATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTGTTTACTGAGTGGTGGGAGTTATTATTTCTCCAGCAGGTGTGACGCCTCCGCCCAGCGTGGGGGCAAAAtta                                                                 >  1:384544/1‑150 (MQ=255)
                                                                                    aTCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCCAAGttat                                                                >  2:299900/1‑150 (MQ=255)
                                                                                    aTCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGttat                                                                >  2:2730668/1‑150 (MQ=255)
                                                                                     tCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTACCCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGttatt                                                               <  1:1165449/150‑1 (MQ=255)
                                                                                     tCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGttatt                                                               <  2:2291099/150‑1 (MQ=255)
                                                                                      ccATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGttatta                                                              <  1:1794735/150‑1 (MQ=255)
                                                                                         tttCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGc                                                           <  1:1540105/150‑1 (MQ=255)
                                                                                           tCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTg                                                         <  2:4213058/150‑1 (MQ=255)
                                                                                            cATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCGTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGGGACGCCTCCGCCCTGCGTGGGGGCAAAGGTATTAAGCTGa                                                        >  2:259500/1‑150 (MQ=255)
                                                                                             aTGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTGGTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGAt                                                       >  1:3523747/1‑150 (MQ=255)
                                                                                             aTGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGAt                                                       <  1:80188/150‑1 (MQ=255)
                                                                                              tGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGAtt                                                      <  2:130237/150‑1 (MQ=255)
                                                                                                cACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTa                                                    <  1:1505656/150‑1 (MQ=255)
                                                                                                 aCGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTAc                                                   >  2:2399862/1‑150 (MQ=255)
                                                                                                 aCGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTAc                                                   <  1:1202348/150‑1 (MQ=255)
                                                                                                  cgggcgggCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACa                                                  <  1:2868538/150‑1 (MQ=255)
                                                                                                  cgggcgggCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACa                                                  <  1:434458/150‑1 (MQ=255)
                                                                                                  cgggcgggCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACa                                                  <  1:487371/150‑1 (MQ=255)
                                                                                                  cgggcgggCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACGGCGGGGTGGCAGTTATTATTTGTCCTGCAGGTGTGACGCCGCCCCCCAGCGGGGGGGCCAAGGTATTAAGCTGATTTaaa                                                  >  1:1408930/1‑148 (MQ=255)
                                                                                                  cgggcgggCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTTATGAGTGTTGGGATTTATTTTTTATCCTGCAGGTGTGTCGCCACCCCCCAGCGGGGGGGCACAGTTATTAAGCTGATTTaaa                                                  >  1:3814151/1‑148 (MQ=255)
                                                                                                   gggcgggcGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAg                                                 <  1:1111854/150‑1 (MQ=255)
                                                                                                   gggcgggcGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAg                                                 <  1:257223/150‑1 (MQ=255)
                                                                                                       gggcgCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCACGAACCAGGGCCGCTTCTTTACTGCGTGATGGCCTTTATGATTTCGCCTGCAGGGGTGACGCCACCGCCCTGCGGGGGGGCCAAGTTATTAAGCTGATTTACAGCCTg                                             >  2:485393/1‑150 (MQ=255)
                                                                                                        ggcgCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTATTTACTTAGTTTTTGCATTTATGTTTTCTACTGCAGGTGTGAAGCCACCGCCCAGCGTGGGGGGAAAGTTATTAATCAGATTTAAATCCTGa                                            >  1:2192977/1‑150 (MQ=255)
                                                                                                           gcAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCATTACTGAGTGATTGCATTTATTATTTGTCCTTCAGGTGTTAAGCCTACGCCCAGCGTGGGGGCAAAGTTATTAAGCTGATTTAAAGCCTGatta                                         >  2:1795221/1‑150 (MQ=255)
                                                                                                                   gtTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGt                                 <  1:4143386/150‑1 (MQ=255)
                                                                                                                    tttCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTg                                <  2:1533388/150‑1 (MQ=255)
                                                                                                                      tCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTATCCTGCAGGTGTGACGCCTCCGCCCAGCGTGGGGGCAAAGTTATTAATCTGATTTACAGCCTGATTATTGTGCGTGAg                              >  2:784673/1‑150 (MQ=255)
                                                                                                                        cAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAggc                            <  1:3163054/150‑1 (MQ=255)
                                                                                                                          gTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTAATGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAggcgg                          >  2:3186231/1‑150 (MQ=255)
                                                                                                                            ccTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCCTTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGgcggcg                        >  2:4382908/1‑150 (MQ=255)
                                                                                                                            ccTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGgcggcg                        <  1:4269432/150‑1 (MQ=255)
                                                                                                                             cTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTGTTTAATTAGTGATGGCAGTTATGTTTTGTCCTGCAGGTGTGACGCCACCGCCCAGCGTGGGGGCAAAGTTATTAAGCTTATTTAAAGCCAGATTATTTTGCGTGAGGCGgcgc                       >  1:2257379/1‑150 (MQ=255)
                                                                                                                             cTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTGTTTAATGAGTGTTGGCATTTATGATTTCTCCAGCAGGTGTGACGCCACCCCCCAGCGTGGGGGCAAAGTTATTAAGCTGATTTAAAGCCAGAATATTGTGCGGGAGGCGgcgc                       >  1:2256691/1‑150 (MQ=255)
                                                                                                                                    aaCTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAAt                <  2:2429148/150‑1 (MQ=255)
                                                                                                                                      cTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATcc              >  2:1940069/1‑150 (MQ=255)
                                                                                                                                           gCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTAt         <  2:2092957/150‑1 (MQ=255)
                                                                                                                                           gCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTAt         <  2:395552/150‑1 (MQ=255)
                                                                                                                                           gCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTAt         <  2:947707/150‑1 (MQ=255)
                                                                                                                                           gCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTAAAGCCTGATTATTGTGCGTGTGGCGGCGCACATAATCCAGTAt         >  2:2167439/1‑150 (MQ=255)
                                                                                                                                               cATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTcac     <  1:2708531/150‑1 (MQ=255)
                                                                                                                                                  gAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGc  <  2:419075/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
CAAACTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGC  >  CP009273/2236952‑2237247

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAACTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGC  >  CP009273/2236953‑2237247
                                                                                                                                                 |                                                                                                                                                     
AAACTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACC                                                                                                                                                   <  SRR19973081.53083/150‑1 (MQ=60)
AAACTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACC                                                                                                                                                   <  SRR19973081.2494791/150‑1 (MQ=60)
              CAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTT                                                                                                                                     <  SRR19973081.3292604/150‑1 (MQ=60)
              CAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTT                                                                                                                                     <  SRR19973081.3292465/150‑1 (MQ=60)
              CAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTT                                                                                                                                     <  SRR19973081.934105/150‑1 (MQ=60)
              CAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTT                                                                                                                                     <  SRR19973081.3649228/150‑1 (MQ=60)
              CAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTT                                                                                                                                     <  SRR19973081.3649221/150‑1 (MQ=60)
              CAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTT                                                                                                                                     <  SRR19973081.3389443/150‑1 (MQ=60)
              CAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTT                                                                                                                                     <  SRR19973081.221008/150‑1 (MQ=60)
                 GTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACT                                                                                                                                  <  SRR19973081.108306/150‑1 (MQ=60)
                 GTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACT                                                                                                                                  <  SRR19973081.154353/150‑1 (MQ=60)
                    GAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAG                                                                                                                               <  SRR19973081.3486631/150‑1 (MQ=60)
                     AGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGT                                                                                                                              <  SRR19973081.2596129/150‑1 (MQ=60)
                       ATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGA                                                                                                                            >  SRR19973081.1295622/1‑150 (MQ=60)
                         TCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCgtgttttatgagtgatt                                                                                                                          >  SRR19973081.851320/1‑133 (MQ=60)
                           AGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGC                                                                                                                        <  SRR19973081.2632789/150‑1 (MQ=60)
                           AGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTAATGAGTGATGGC                                                                                                                        >  SRR19973081.2323724/1‑150 (MQ=60)
                           AGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCGTCTTTACTGtgtgttggg                                                                                                                        >  SRR19973081.2035213/1‑141 (MQ=60)
                             CAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCAT                                                                                                                      <  SRR19973081.426641/150‑1 (MQ=60)
                               GCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTT                                                                                                                    <  SRR19973081.1499143/150‑1 (MQ=60)
                                CTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATTGCATTTA                                                                                                                   >  SRR19973081.627731/1‑150 (MQ=60)
                                CTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTA                                                                                                                   <  SRR19973081.3127300/150‑1 (MQ=60)
                                CTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTA                                                                                                                   <  SRR19973081.680908/150‑1 (MQ=60)
                                CTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCGTTACTGAGTGATGGCATTTA                                                                                                                   >  SRR19973081.3073570/1‑150 (MQ=60)
                                 TGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTAT                                                                                                                  <  SRR19973081.224112/150‑1 (MQ=60)
                                 TGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTAT                                                                                                                  <  SRR19973081.2668518/150‑1 (MQ=60)
                                  GCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATG                                                                                                                 <  SRR19973081.248461/150‑1 (MQ=60)
                                    ATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTGTTTAATGAGTGGTTGCAGTTATGAT                                                                                                               >  SRR19973081.438043/1‑150 (MQ=60)
                                    ATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTTATGGCAGTTATGAT                                                                                                               >  SRR19973081.523630/1‑150 (MQ=60)
                                         TTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTATTTACTGAGTGATTGCATTTATGATTTCTC                                                                                                          >  SRR19973081.4021509/1‑150 (MQ=60)
                                           CGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCgtgttttattagttttggcagttatgatttatgca                                                                                                        >  SRR19973081.29799/1‑115 (MQ=60)
                                             GTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTgttttatgagtgttggcctttatgttttatccagc                                                                                                      >  SRR19973081.2638738/1‑115 (MQ=60)
                                             GTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTAAAGAGTGATGGCAATTATGATTTCTacatc                                                                                                      >  SRR19973081.2797146/1‑145 (MQ=60)
                                                   TGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTTCTTAGTGTTTGGATTTATGATTTCTACTGCAGGTGT                                                                                                >  SRR19973081.3411319/1‑150 (MQ=60)
                                                   TGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGT                                                                                                <  SRR19973081.1131029/150‑1 (MQ=60)
                                                    GACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTG                                                                                               <  SRR19973081.337938/150‑1 (MQ=60)
                                                          CAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCT                                                                                         <  SRR19973081.2103031/150‑1 (MQ=60)
                                                          CAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCT                                                                                         <  SRR19973081.2102976/150‑1 (MQ=60)
                                                            ATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTGTTTACTGAGTGATGGCAGTTATTATTTCTCCTGCAGGTGTGTCGCCTCC                                                                                       >  SRR19973081.3652558/1‑150 (MQ=60)
                                                            ATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATTATTTCTCCTGCAGGTGTGACGCCTCC                                                                                       >  SRR19973081.3652545/1‑150 (MQ=60)
                                                            ATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTATTTACAGAGTGtttggaattatgttttatccatcaggtttgtcgccacc                                                                                       >  SRR19973081.1663688/1‑112 (MQ=60)
                                                              AAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTAATGAGTGATGGCATTTATGATTTCTCCAGCAGGTGTGACGCCacccc                                                                                     >  SRR19973081.3085483/1‑145 (MQ=60)
                                                                  CTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCgtgtttaattagtgatgggatttaatatttatacatcagggtttaagccaccccccag                                                                                 >  SRR19973081.898743/1‑92 (MQ=60)
                                                                   TTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACAGAGTGATGGCATTTATGATTTCTCCTGCAGGGGTGACGCCTCCCCCCAGC                                                                                >  SRR19973081.4730216/1‑150 (MQ=60)
                                                                    TTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCG                                                                               <  SRR19973081.547462/150‑1 (MQ=60)
                                                                    TTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCG                                                                               <  SRR19973081.546584/150‑1 (MQ=60)
                                                                     TTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGT                                                                              <  SRR19973081.3477354/150‑1 (MQ=60)
                                                                          CGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCGTGTTTACTGAGTTATTGCATTTATTATTTATCCTTCAGGTGTGACGCCACCCCCCAGCGTGGGGG                                                                         >  SRR19973081.125247/1‑150 (MQ=60)
                                                                             TTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAA                                                                      <  SRR19973081.1170671/150‑1 (MQ=60)
                                                                                GTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTGTTTACTGTGTTTTGGGCTTTATGATTTATCCTGCAGGGGTGACGCCACCCCCCTGCGGGGGGGCAAAGT                                                                   >  SRR19973081.4689583/1‑150 (MQ=60)
                                                                                 TTATCCATTTCCTGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTT                                                                  <  SRR19973081.4204513/150‑1 (MQ=60)
                                                                                 TTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTT                                                                  <  SRR19973081.3141781/150‑1 (MQ=60)
                                                                                  TATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATTGGATTTTTGATTTGTCCTGCAGGTGTGAAGCCACCCCCCAGCGTGGGGGCCAAGTTA                                                                 >  SRR19973081.2632148/1‑150 (MQ=60)
                                                                                  TATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCGTCGTTACTGAGTGTTGGCATTTATGATTTCTCCAGCAGGTGTGACGCCTCCGCCCAGCGTGGGGGCCAAGTTA                                                                 >  SRR19973081.2633502/1‑150 (MQ=60)
                                                                                  TATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTGTTTACTGAGTGGTGGGAGTTATTATTTCTCCAGCAGGTGTGACGCCTCCGCCCAGCGTGGGGGCAAAATTA                                                                 >  SRR19973081.387050/1‑150 (MQ=60)
                                                                                  TATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGTTGGGCTTTTTGATTTATCCTGCAGGTGTGACGCCACCGCCCAGCGTGGGGGGCAAGTTA                                                                 >  SRR19973081.387057/1‑150 (MQ=60)
                                                                                   ATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCgtggtttatgggtgttggggtttttggtttgtactgccggggtgtcgccgccccccagcgtggggggccaggtat                                                                >  SRR19973081.60029/1‑75 (MQ=60)
                                                                                    TCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTACCCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATT                                                               <  SRR19973081.1172941/150‑1 (MQ=60)
                                                                                    TCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATT                                                               <  SRR19973081.1173366/150‑1 (MQ=60)
                                                                                     CCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCGTCTTTACTGAGTGTTGGCATTTATTATTTCTACAGCAGGTGTGtcgccaccccccagccgggggggcaagttatta                                                              >  SRR19973081.932352/1‑117 (MQ=60)
                                                                                     CCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCGTCTTTACAGAGTGTTGGGATTTATTATTTATACAGCAGGGGTGACGCCTCCGCCCAGCGGGGGGGGAAATTTATTT                                                              >  SRR19973081.1335861/1‑150 (MQ=60)
                                                                                     CCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTA                                                              <  SRR19973081.4274091/150‑1 (MQ=60)
                                                                                     CCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTA                                                              <  SRR19973081.1806464/150‑1 (MQ=60)
                                                                                        TTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGC                                                           <  SRR19973081.3540384/150‑1 (MQ=60)
                                                                                        TTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGC                                                           <  SRR19973081.1550148/150‑1 (MQ=60)
                                                                                          TCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCGTCTTTAaagggtgttgggaattaggatttagccagcaggggtgaccccaccgcccagcgggggggcccaggtattaagctg                                                         >  SRR19973081.605193/1‑75 (MQ=60)
                                                                                            ATGCACGGGCGGGCGCAGCGGTGTTTCCAGTTCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGAT                                                       <  SRR19973081.83502/150‑1 (MQ=60)
                                                                                            ATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTGGTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCACCGCCCAGCGTGGGGGCAAAGTTATTAATCTGAT                                                       >  SRR19973081.3545293/1‑150 (MQ=60)
                                                                                            ATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGAT                                                       <  SRR19973081.81073/150‑1 (MQ=60)
                                                                                               CACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTA                                                    <  SRR19973081.1515585/150‑1 (MQ=60)
                                                                                                ACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTAC                                                   <  SRR19973081.3685991/150‑1 (MQ=60)
                                                                                                ACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTAC                                                   <  SRR19973081.1210069/150‑1 (MQ=60)
                                                                                                 CGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACA                                                  <  SRR19973081.490813/150‑1 (MQ=60)
                                                                                                 CGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACA                                                  <  SRR19973081.2886067/150‑1 (MQ=60)
                                                                                                 CGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACA                                                  <  SRR19973081.437378/150‑1 (MQ=60)
                                                                                                 CGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACGGCGGGGTGGCAGTTATTATTTGTCCTGCAGGTGTGACGCCGCCCCCCAGCGGGGGGGCCAAGGTATTAAGCTGATTTAAA                                                  >  SRR19973081.1418362/1‑150 (MQ=60)
                                                                                                 CGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTTATGAGTGTTGGGATTTATTTTTTATCCTGCAGGTGTGTCGCCACCCCCCAGCGGGGGGGCACAGTTATTAAGCTGATTTAAA                                                  >  SRR19973081.3837596/1‑150 (MQ=60)
                                                                                                  GGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAG                                                 <  SRR19973081.259306/150‑1 (MQ=60)
                                                                                                  GGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAG                                                 <  SRR19973081.1119213/150‑1 (MQ=60)
                                                                                                  GGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAG                                                 <  SRR19973081.1119158/150‑1 (MQ=60)
                                                                                                       GGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTATTTACTTAGTTTTTGCATTTATGTTTTCTACTGCAGGTGTGAAGCCACCGCCCAGCGTGGGGGGAAAGTTATTAATCAGATTTAAATCCTGA                                            >  SRR19973081.2206929/1‑150 (MQ=60)
                                                                                                           CAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTAATGAGTGttgtgagtttttatttatacagcaggtgtgtagccaccccccagcgtggggggcaagttattaatatgatttaaaaccagattat                                        >  SRR19973081.3332162/1‑65 (MQ=60)
                                                                                                           CAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCGTGTGTACTTAGTGATTGGGTTTTTTATTTCTACAGCAGGTGTGTCGCCACCCCCCAGCGGGGGGGGGAAGTTATTAAGCTGATTTACAGCCAGATTAT                                        >  SRR19973081.3332215/1‑150 (MQ=38)
                                                                                                               GGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCGTGTTTAATGAGGGATGGCATgttttttttatacatcaggtgtgaagccaccccccagcggggggggccagttattaataggttttaaagccagattattttt                                    >  SRR19973081.3268828/1‑68 (MQ=54)
                                                                                                                  GTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGT                                 <  SRR19973081.4168682/150‑1 (MQ=60)
                                                                                                                       CAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGC                            <  SRR19973081.3182323/150‑1 (MQ=60)
                                                                                                                           CCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCG                        <  SRR19973081.4300536/150‑1 (MQ=60)
                                                                                                                           CCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCG                        <  SRR19973081.4855585/150‑1 (MQ=60)
                                                                                                                           CCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCG                        <  SRR19973081.4295468/150‑1 (MQ=60)
                                                                                                                            CTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTGTTTAATTAGTGATGGCAGTTATGTTTTGTCCTGCAGGTGTGACGCCACCGCCCAGCGTGGGGGCAAAGTTATTAAGCTTATTTAAAGCCAGATTATTTTGCGTGAGGCGGCGC                       >  SRR19973081.2271643/1‑150 (MQ=60)
                                                                                                                            CTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTGTTTAATGAGTGTTGGCATTTATGATTTCTCCAGCAGGTGTGACGCCACCCCCCAGCGTGGGGGCAAAGTTATTAAGCTGATTTAAAGCCAGAATATTGTGCGGGAGGCGGCGC                       >  SRR19973081.2270954/1‑150 (MQ=60)
                                                                                                                            CTCGCGCAACTAACGCTTCATTAACCAGGGCCGCGGCTTTACTGAGTGGTGGCCTTTATTCTTTCTCCAGCAGGTGTGACGCCTCCCCCCAGCGGGGGGGCCAAGGTATTAAGCTGATTTACAGCCTGATTATTTTGCGTGAGGCGGCGC                       >  SRR19973081.2271057/1‑150 (MQ=60)
                                                                                                                                 GCAACTAACGCTTCATGAACCAGGGCCGCTTGTTTTATGAGTGATGGGATTTATTATTTGTCCTGCAGGTGTGACGCCACCCCCCAGCGTGGGGGCCAAGTTATTAATATGATTTAAAGCCAGATTATTGTGCGTGTGGCGGCGCACCTA                  >  SRR19973081.2742004/1‑150 (MQ=60)
                                                                                                                                   AACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAAT                <  SRR19973081.28496/150‑1 (MQ=60)
                                                                                                                                    ACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATC               <  SRR19973081.4080100/150‑1 (MQ=60)
                                                                                                                                       AACGCTTCATTAACCAGGGCCGCTTCTTTACTTGGTTATGGCATTTATTATTTCTCCTGCAGGTGTTTCGCCAACCCCCAGCGTGGGGGCAAAGTTATTAAAATGATTTTAAGCCATATTATTGTGCGTGAGGCGGCGCcactaaaacag            >  SRR19973081.4821336/1‑139 (MQ=46)
                                                                                                                                         CGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTA          <  SRR19973081.4717805/150‑1 (MQ=60)
                                                                                                                                             TCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCA      <  SRR19973081.1817255/150‑1 (MQ=60)
                                                                                                                                              CATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCAC     <  SRR19973081.2725279/150‑1 (MQ=60)
                                                                                                                                                TTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAG   <  SRR19973081.1598002/150‑1 (MQ=60)
                                                                                                                                                 TAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGC  <  SRR19973081.4314106/150‑1 (MQ=60)
                                                                                                                                                 TAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGC  <  SRR19973081.4314123/150‑1 (MQ=60)
                                                                                                                                                 |                                                                                                                                                     
AAACTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGC  >  CP009273/2236953‑2237247

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑