Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,237,098 G→T 100% H12N (CAT→AAT)  folE ← GTP cyclohydrolase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,237,0980GT98.7% 722.4 / 0.5 227H12N (CAT→AAT) folEGTP cyclohydrolase I
Reads supporting (aligned to +/- strand):  ref base G (3/0);  new base T (99/123);  total (104/123)
Fisher's exact test for biased strand distribution p-value = 9.17e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 227 total aligned reads displayed.

AAACTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGT  >  CP009273/2236953‑2237238
                                                                                                                                                 |                                                                                                                                            
aaaCTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTaa                                                                                                                                            <  2:967421/148‑1 (MQ=255)
aaaCTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTaa                                                                                                                                            >  2:1342370/1‑148 (MQ=255)
  ccccccatcaCCCAGCTCGAGACGCACCAGCTCCATGATTTCGGTCATATGACCAGCAAGAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAAcc                                                                                                                                          <  1:1875261/143‑1 (MQ=255)
  aCTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAAcc                                                                                                                                          >  2:3489228/1‑148 (MQ=255)
      tcatcaGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAggg                                                                                                                                      >  1:1777336/1‑148 (MQ=255)
      tcatcaGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAggg                                                                                                                                      <  2:1136733/148‑1 (MQ=255)
      tcatcaGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAggg                                                                                                                                      >  1:1704941/1‑148 (MQ=255)
         tcaGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCg                                                                                                                                   <  1:3246978/148‑1 (MQ=255)
             ccAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGcttc                                                                                                                               <  2:3161640/148‑1 (MQ=255)
             ccAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGcttc                                                                                                                               >  2:2655311/1‑148 (MQ=255)
             ccAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGcttc                                                                                                                               <  2:1946620/148‑1 (MQ=255)
              cAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGcttct                                                                                                                              <  1:1529875/148‑1 (MQ=255)
               aGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGcttctt                                                                                                                             >  1:132667/1‑148 (MQ=255)
                 gTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTa                                                                                                                           <  1:1048916/148‑1 (MQ=255)
                 gTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTa                                                                                                                           <  1:820429/148‑1 (MQ=255)
                  tCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATAAACCAGGGCCGCGTGTTTaa                                                                                                                          >  1:1263429/1‑147 (MQ=255)
                      gaTTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAg                                                                                                                      <  1:1087200/148‑1 (MQ=255)
                         tCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGa                                                                                                                   <  2:2066160/148‑1 (MQ=255)
                         tCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGa                                                                                                                   <  2:2066056/148‑1 (MQ=255)
                         tCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTAATGAGTGa                                                                                                                   >  1:2585365/1‑148 (MQ=255)
                         tCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTATTTAATGAGTGa                                                                                                                   >  1:356509/1‑148 (MQ=255)
                          cagcagCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGAt                                                                                                                  <  2:1317113/148‑1 (MQ=255)
                          cagcagCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGAt                                                                                                                  <  2:2795237/148‑1 (MQ=255)
                            gcagcTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATgg                                                                                                                >  2:787271/1‑148 (MQ=255)
                               gcTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCAt                                                                                                             >  2:1124937/1‑148 (MQ=255)
                               gcTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCAt                                                                                                             <  1:2617457/148‑1 (MQ=255)
                                cTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCAtt                                                                                                            <  2:3400978/148‑1 (MQ=255)
                                  gCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTa                                                                                                          <  1:3522401/148‑1 (MQ=255)
                                   cATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTTCTTAGTGATTGGATTTAt                                                                                                         >  1:2458640/1‑148 (MQ=255)
                                    aTGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATg                                                                                                        <  1:2607237/148‑1 (MQ=255)
                                     tGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGa                                                                                                       <  1:2727008/148‑1 (MQ=255)
                                     tGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGa                                                                                                       <  1:278583/148‑1 (MQ=255)
                                     tGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGa                                                                                                       <  1:284713/148‑1 (MQ=255)
                                     tGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGa                                                                                                       <  1:1130334/148‑1 (MQ=255)
                                     tGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGa                                                                                                       <  2:1616641/148‑1 (MQ=255)
                                       aTTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGAtt                                                                                                     >  2:151889/1‑148 (MQ=255)
                                        tttCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGAttt                                                                                                    <  2:2963117/148‑1 (MQ=255)
                                        tttCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGAttt                                                                                                    <  1:2009746/148‑1 (MQ=255)
                                         ttCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTtc                                                                                                   <  1:2655311/148‑1 (MQ=255)
                                         ttCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGAGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGAtttg                                                                                                   >  2:221499/1‑147 (MQ=255)
                                            ggTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTcc                                                                                                <  1:96962/148‑1 (MQ=255)
                                             gTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCAt                                                                                                             <  2:2089441/134‑1 (MQ=255)
                                             gTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCAt                                                                                                             >  1:2089441/1‑134 (MQ=255)
                                               cATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCGTCTTTACTGAGTGATTGGCTTTATGATTTATCCTGc                                                                                             >  1:767730/1‑148 (MQ=255)
                                                  atGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAgg                                                                                          >  2:2600067/1‑148 (MQ=255)
                                                  atGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAgg                                                                                          >  2:405335/1‑148 (MQ=255)
                                                  atGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAgg                                                                                          <  1:822749/148‑1 (MQ=255)
                                                   tGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGgt                                                                                         >  2:2153094/1‑148 (MQ=255)
                                                   tGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGgt                                                                                         <  1:1078751/148‑1 (MQ=255)
                                                   tGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGgt                                                                                         <  1:3177572/148‑1 (MQ=255)
                                                    gACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCCTTTATGTTTTCTCCTGCAGgtg                                                                                        >  1:555873/1‑148 (MQ=255)
                                                    gACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGgtg                                                                                        <  1:1230084/148‑1 (MQ=255)
                                                     aCCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCGGCAGgtgt                                                                                       >  2:1280751/1‑148 (MQ=255)
                                                      ccATCAATAAGGCTTTTGCGCGTTTCGTGATCCATTTCATGCTCGGGCGGTCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCTCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGtgtg                                                                                      <  1:3000148/148‑1 (MQ=255)
                                                       cAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGa                                                                                     <  2:1469992/148‑1 (MQ=255)
                                                         gCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGTGTGATGGCATTTATGATTTCTCCAGAAGGTGTGACg                                                                                   >  2:734259/1‑148 (MQ=255)
                                                            aTAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCt                                                                                <  2:301813/148‑1 (MQ=255)
                                                             tAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTc                                                                               <  1:858770/148‑1 (MQ=255)
                                                                ggCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATTATTTATCCATCAGGTGTGACGCCTCCGc                                                                            >  1:1437987/1‑148 (MQ=255)
                                                                ggCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGc                                                                            <  2:1783796/148‑1 (MQ=255)
                                                                ggCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGc                                                                            >  2:2401754/1‑148 (MQ=255)
                                                                 gCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTTAGTTATGGGATTTATGATTTATCCTGAAGGTGTGACGCCTCCGcc                                                                           >  1:3396477/1‑148 (MQ=255)
                                                                 gCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGcc                                                                           <  2:3411793/148‑1 (MQ=255)
                                                                 gCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGcc                                                                           >  2:1489811/1‑148 (MQ=255)
                                                                 gCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTAATTAGTGATTGGATTTATGATTTATCCTGCAGGTGTGACGCCTCCGcc                                                                           >  1:742869/1‑148 (MQ=255)
                                                                  cTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGccc                                                                          <  1:496154/148‑1 (MQ=255)
                                                                    tttGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTg                                                                        >  2:1000243/1‑148 (MQ=255)
                                                                          cgTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTggg                                                                  >  1:2034369/1‑148 (MQ=255)
                                                                          cgTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTggg                                                                  >  2:2718667/1‑148 (MQ=255)
                                                                             ttCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTATCCTGCAGGTGTGACGCCTCCGCCCAGCGTGGGGGc                                                               >  1:2353705/1‑148 (MQ=255)
                                                                                 ttATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAg                                                           >  1:2275376/1‑148 (MQ=255)
                                                                                 ttATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAg                                                           >  2:1761376/1‑148 (MQ=255)
                                                                                  tATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGt                                                          >  2:1684234/1‑148 (MQ=255)
                                                                                    tCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGtta                                                        >  2:2514567/1‑148 (MQ=255)
                                                                                     ccATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGttat                                                       >  2:2322215/1‑148 (MQ=255)
                                                                                       aTTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGttatta                                                     <  1:348679/148‑1 (MQ=255)
                                                                                        tttCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTaa                                                    <  1:2226654/148‑1 (MQ=255)
                                                                                            aTGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTg                                                <  1:2153094/148‑1 (MQ=255)
                                                                                              gCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGAt                                              <  2:864059/148‑1 (MQ=255)
                                                                                               cACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGAtt                                             <  2:2895752/148‑1 (MQ=255)
                                                                                                    gcgggcgCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCAGCCGGTGTGACGCCTCCGCCCAGCGTTGGGGCAAAGTTATTAAGCTGATTTACAg                                        >  2:79549/1‑148 (MQ=255)
                                                                                                            aGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGTCTGatta                                <  2:3164132/148‑1 (MQ=255)
                                                                                                            aGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGTCTGatta                                <  2:3165917/148‑1 (MQ=255)
                                                                                                             gCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGattat                               <  2:1777291/148‑1 (MQ=255)
                                                                                                               ggTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATtg                             <  2:432457/148‑1 (MQ=255)
                                                                                                                    ttCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTTATGGCATTTATTATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGGGGGGGGGCCAAGTTATTAAGCTGTTTTACAGCCTGATTATTTTGCGt                        >  2:1325420/1‑148 (MQ=255)
                                                                                                                    ttCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCGTGTTTTGTGTGTGTTTGGGTTTATGATTTATCCTGCAGGGGTGTCGCCACCCCCCAGCGGGGGGGCCCAGTTATTAATCTGATTTAAATCCAGATTATTTTGGGt                        >  1:3303165/1‑148 (MQ=255)
                                                                                                                     tCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTg                       <  2:2305079/148‑1 (MQ=255)
                                                                                                                        aGTCCTCGCGCAACTACCGCGTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGAGTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAgg                    <  2:3285361/148‑1 (MQ=255)
                                                                                                                          tCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCGTTAATGAGTGATGGCCTTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGGTATTAAGCTGATTTACAGCCAGATTATTGTGGGTGAggcg                  >  1:2885466/1‑148 (MQ=255)
                                                                                                                                  cAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACAt          >  2:3222795/1‑148 (MQ=255)
                                                                                                                                  cAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACAt          <  1:1607222/148‑1 (MQ=255)
                                                                                                                                  cAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACAt          <  1:1761461/148‑1 (MQ=255)
                                                                                                                                   aaCTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATa         <  1:614096/148‑1 (MQ=255)
                                                                                                                                    aCTAACGCTTCATTAACCAGGGCCGCTTGTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGGGTGACGCCGCCCCCCAGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCGGATTATTTTTCGTGAGGCGGCGCACaaaa        >  1:2283221/1‑148 (MQ=255)
                                                                                                                                     cTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGTTGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGGGGGGGCCAAGTTATTAATCTGATTTAAAGCCAGATTATTTTGCGTGAGGCGGCGCACATaaa       >  2:3327271/1‑147 (MQ=255)
                                                                                                                                     cTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAAt       <  1:1684234/148‑1 (MQ=255)
                                                                                                                                      tAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGGGGCGCACATAAta      >  2:3386142/1‑147 (MQ=255)
                                                                                                                                      tAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGGGGGGGCAAAGGTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATc      >  2:1601895/1‑148 (MQ=255)
                                                                                                                                          gCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGt  <  2:1704941/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                            
AAACTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGT  >  CP009273/2236953‑2237238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CATCAAACTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAG  >  CP009273/2236949‑2237237
                                                                                                                                                     |                                                                                                                                           
CATCAAACTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATT                                                                                                                                             <  SRR19973066.3041783/150‑1 (MQ=60)
CATCAAACTGTCATCAGACAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGAACAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATT                                                                                                                                             <  SRR19973066.1254334/150‑1 (MQ=60)
   CAAACTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAAC                                                                                                                                          <  SRR19973066.1676337/150‑1 (MQ=60)
   CAAACTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGAGCAACTAACGCTTCATTAAC                                                                                                                                          <  SRR19973066.3318273/150‑1 (MQ=60)
   CAAACTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGAGCAACTAACGCTTCATTAAC                                                                                                                                          <  SRR19973066.3318248/150‑1 (MQ=60)
     AACTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCTCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCA                                                                                                                                        >  SRR19973066.1759869/1‑150 (MQ=60)
     AACTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCA                                                                                                                                        <  SRR19973066.2121248/150‑1 (MQ=60)
      ccccccatcacccagctcgagacgcacCAGCTCCATGATTTCGGTCATATGACCAGCAAGAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAG                                                                                                                                       <  SRR19973066.1888649/123‑1 (MQ=60)
      ACTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAG                                                                                                                                       <  SRR19973066.2094626/150‑1 (MQ=60)
      ACTGACATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCACCTAACGCTTCATTAACCAG                                                                                                                                       >  SRR19973066.3024021/1‑150 (MQ=60)
        TGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGG                                                                                                                                     >  SRR19973066.1717316/1‑150 (MQ=60)
        TGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGG                                                                                                                                     >  SRR19973066.1790000/1‑150 (MQ=60)
        TGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGG                                                                                                                                     >  SRR19973066.1790046/1‑150 (MQ=60)
          TCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCC                                                                                                                                   <  SRR19973066.1891183/150‑1 (MQ=60)
          TCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCC                                                                                                                                   <  SRR19973066.1890996/150‑1 (MQ=60)
          TCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCC                                                                                                                                   <  SRR19973066.584753/150‑1 (MQ=60)
          TCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCC                                                                                                                                   <  SRR19973066.2293992/150‑1 (MQ=60)
          TCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGATTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCC                                                                                                                                   >  SRR19973066.739998/1‑150 (MQ=60)
           CATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCG                                                                                                                                  >  SRR19973066.99796/1‑150 (MQ=60)
           CATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCG                                                                                                                                  >  SRR19973066.99809/1‑150 (MQ=60)
             TCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCT                                                                                                                                <  SRR19973066.3267640/150‑1 (MQ=60)
             TCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCT                                                                                                                                <  SRR19973066.3268380/150‑1 (MQ=60)
                 CCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTT                                                                                                                            >  SRR19973066.133964/1‑150 (MQ=60)
                  CAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTT                                                                                                                           <  SRR19973066.1540739/150‑1 (MQ=60)
                  CAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTT                                                                                                                           <  SRR19973066.1540819/150‑1 (MQ=60)
                  CAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTT                                                                                                                           <  SRR19973066.245379/150‑1 (MQ=60)
                    GGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATAAACCAGGGCCGCgtgtttaa                                                                                                                         >  SRR19973066.1272550/1‑142 (MQ=60)
                    GGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATAAACCAGGGCCGCGTCTTTAA                                                                                                                         >  SRR19973066.1271610/1‑150 (MQ=60)
                     GTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACT                                                                                                                        <  SRR19973066.1056625/150‑1 (MQ=60)
                     GTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACT                                                                                                                        <  SRR19973066.2573144/150‑1 (MQ=60)
                     GTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACT                                                                                                                        <  SRR19973066.826427/150‑1 (MQ=60)
                      TCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTG                                                                                                                       <  SRR19973066.351923/150‑1 (MQ=60)
                       CGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGA                                                                                                                      <  SRR19973066.129153/150‑1 (MQ=60)
                        GAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAG                                                                                                                     <  SRR19973066.3235746/150‑1 (MQ=60)
                          GATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTG                                                                                                                   <  SRR19973066.1095132/150‑1 (MQ=60)
                           ATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTAATGAGTGA                                                                                                                  >  SRR19973066.2602885/1‑150 (MQ=60)
                           ATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTATTTAATGAGTGA                                                                                                                  >  SRR19973066.359287/1‑150 (MQ=60)
                           ATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCGTCTTTAATGAGTGT                                                                                                                  >  SRR19973066.2418008/1‑150 (MQ=60)
                                   GCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTT                                                                                                          <  SRR19973066.2635133/150‑1 (MQ=60)
                                   GCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTT                                                                                                          <  SRR19973066.2634824/150‑1 (MQ=60)
                                     TGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTTCTTAGTGATTGGATTTAT                                                                                                        >  SRR19973066.2475398/1‑150 (MQ=60)
                                      GCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATG                                                                                                       <  SRR19973066.3545850/150‑1 (MQ=60)
                                      GCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATG                                                                                                       >  SRR19973066.1810399/1‑150 (MQ=60)
                                       CATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGA                                                                                                      >  SRR19973066.2333837/1‑150 (MQ=60)
                                        ATGATTTCGGTCATATGACCAGCAATATGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGAT                                                                                                     <  SRR19973066.548255/150‑1 (MQ=60)
                                        ATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGAT                                                                                                     <  SRR19973066.2624864/150‑1 (MQ=60)
                                        ATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGAT                                                                                                     <  SRR19973066.2625097/150‑1 (MQ=60)
                                         TGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATT                                                                                                    <  SRR19973066.286769/150‑1 (MQ=60)
                                         TGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATT                                                                                                    <  SRR19973066.280613/150‑1 (MQ=60)
                                         TGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATT                                                                                                    <  SRR19973066.2745346/150‑1 (MQ=60)
                                         TGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATT                                                                                                    <  SRR19973066.1138447/150‑1 (MQ=60)
                                         TGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATT                                                                                                    <  SRR19973066.1506124/150‑1 (MQ=60)
                                            TTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCT                                                                                                 <  SRR19973066.2023836/150‑1 (MQ=60)
                                             TTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTC                                                                                                <  SRR19973066.2673119/150‑1 (MQ=60)
                                             TTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTC                                                                                                <  SRR19973066.2239701/150‑1 (MQ=60)
                                               CGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCGTTACTGAGTGtttgcatttagatcggaacag                                                                                              >  SRR19973066.2103841/1‑129 (MQ=60)
                                                GGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTG                                                                                             <  SRR19973066.98004/150‑1 (MQ=60)
                                                GGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTG                                                                                             <  SRR19973066.97999/150‑1 (MQ=60)
                                                GGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTG                                                                                             <  SRR19973066.405602/150‑1 (MQ=60)
                                                 GTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTGTTTAATGAGTGATGGCATTTATTTTTTCTCCTGC                                                                                            >  SRR19973066.773414/1‑150 (MQ=60)
                                                 GTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCGTCTTTACTGAGTGATTGGCTTTATGATTTATCCTGC                                                                                            >  SRR19973066.773417/1‑150 (MQ=60)
                                                  TCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCA                                                                                           <  SRR19973066.1057043/150‑1 (MQ=60)
                                                   CATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAG                                                                                          <  SRR19973066.2821900/150‑1 (MQ=60)
                                                      ATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCCTTTATGTTTTCTCCTGCAGGTG                                                                                       >  SRR19973066.559860/1‑150 (MQ=60)
                                                      ATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTG                                                                                       <  SRR19973066.828756/150‑1 (MQ=60)
                                                      ATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTG                                                                                       <  SRR19973066.828711/150‑1 (MQ=60)
                                                       TGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGT                                                                                      <  SRR19973066.1086637/150‑1 (MQ=60)
                                                       TGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGT                                                                                      <  SRR19973066.1087034/150‑1 (MQ=60)
                                                       TGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGT                                                                                      <  SRR19973066.3198718/150‑1 (MQ=60)
                                                       TGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGT                                                                                      <  SRR19973066.2877002/150‑1 (MQ=60)
                                                        GACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTG                                                                                     <  SRR19973066.1238822/150‑1 (MQ=60)
                                                        GACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTG                                                                                     <  SRR19973066.20413/150‑1 (MQ=60)
                                                          CCATCAATAAGGCTTTTGCGCGTTTCGTGATCCATTTCATGCTCGGGCGGTCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCTCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGAC                                                                                   <  SRR19973066.3020070/150‑1 (MQ=60)
                                                          CCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGAC                                                                                   <  SRR19973066.2617664/150‑1 (MQ=60)
                                                               AATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTC                                                                              <  SRR19973066.2983584/150‑1 (MQ=60)
                                                                ATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCC                                                                             <  SRR19973066.1133032/150‑1 (MQ=60)
                                                                 TAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCG                                                                            <  SRR19973066.864833/150‑1 (MQ=60)
                                                                 TAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCG                                                                            <  SRR19973066.864956/150‑1 (MQ=60)
                                                                  AAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATTATTTATCCATCAGGTGTGACGCCTCCGC                                                                           >  SRR19973066.1448231/1‑150 (MQ=60)
                                                                   AGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTTAGTTATGGGATTTATGATTTATCCTGAAGGTGTGACGCCTCCGCC                                                                          >  SRR19973066.3419136/1‑150 (MQ=60)
                                                                   AGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTAATTAGTGATTGGATTTATGATTTATCCTGCAGGTGTGACGCCTCCGCC                                                                          >  SRR19973066.748384/1‑150 (MQ=60)
                                                                   AGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCGTCTTTTCTGAGTGATGGCATTTATTATTTATCCTTCAGGTGTGACGCCTCCGCC                                                                          >  SRR19973066.2173969/1‑150 (MQ=60)
                                                                     GCTTTTGCGCGTTTCGTGATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCT                                                                        <  SRR19973066.2813646/150‑1 (MQ=60)
                                                                      CTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTG                                                                       <  SRR19973066.499854/150‑1 (MQ=60)
                                                                        TTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCgtgtttaatgagttattggagttattttttatacatcaggtgtgtcgccaacccccagcg                                                                     >  SRR19973066.930978/1‑90 (MQ=60)
                                                                            CGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGG                                                                 >  SRR19973066.2048563/1‑150 (MQ=60)
                                                                               GTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGC                                                              <  SRR19973066.78386/150‑1 (MQ=60)
                                                                               GTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGC                                                              <  SRR19973066.78359/150‑1 (MQ=60)
                                                                               GTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTATCCTGCAGGTGTGACGCCTCCGCCCAGCGTGGGGGC                                                              >  SRR19973066.2369689/1‑150 (MQ=60)
                                                                                TTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCGCCGCCCTGCGTGGGGGCA                                                             >  SRR19973066.264398/1‑150 (MQ=60)
                                                                                   CGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAG                                                          >  SRR19973066.2291010/1‑150 (MQ=60)
                                                                                   CGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCGTCTTTACTGAGTGATGGCCTGTATGATTTCTCCTGCAGGGGTGACGCCACCGCCCGGCGTGGGGGCAAAG                                                          >  SRR19973066.2290925/1‑150 (MQ=60)
                                                                                       ATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCTCAACTAACGCTTCATTAACCAGGGCCGCTTgttttcggcgtgttgggggttttgatttgtcctgcaggtggttcgccgccccccagcgggggggccaagttat                                                      >  SRR19973066.3423970/1‑77 (MQ=60)
                                                                                        TCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATT                                                     <  SRR19973066.529756/150‑1 (MQ=60)
                                                                                           ATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAG                                                  <  SRR19973066.351397/150‑1 (MQ=60)
                                                                                            TTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGC                                                 <  SRR19973066.2242041/150‑1 (MQ=60)
                                                                                             TTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCT                                                <  SRR19973066.3544303/150‑1 (MQ=60)
                                                                                                ATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGAT                                             <  SRR19973066.2168041/150‑1 (MQ=60)
                                                                                                     CGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACA                                        <  SRR19973066.1149127/150‑1 (MQ=60)
                                                                                                      GGGCGGGCGCAGCTGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAG                                       <  SRR19973066.1519164/150‑1 (MQ=60)
                                                                                                          GGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTGTTTACTGAGTGATGGCATTTATGTTTTCTACTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTG                                   >  SRR19973066.276287/1‑150 (MQ=60)
                                                                                                            GCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGAT                                 <  SRR19973066.1360598/150‑1 (MQ=60)
                                                                                                             CGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATT                                <  SRR19973066.1695521/150‑1 (MQ=60)
                                                                                                                   GGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCgtggtttatgtgtggtgggggttttgttttctcatgcaggggtgtggccgcccccccgcggggggggcaagttatttatctgttttaaagccagatttttttg                          >  SRR19973066.1216189/1‑47 (MQ=60)
                                                                                                                   GGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCGGGGTTACTGAGTGATGGGAGTTTTGATTTATCCAGCAGGTGTGTCCCCGCCCCCCCGGGTGGGGGGCAAGTTATTAATCTGATTTTAAGCCTGATTATTTTG                          >  SRR19973066.1216107/1‑150 (MQ=27)
                                                                                                                      GTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCgtgttttgtgtgtgtttgggtttatgatttatcctgcaggggtgtcgccaccccccagcgggggggcccagttattaatctgatttaaatccagattattttgggt                       >  SRR19973066.3325016/1‑44 (MQ=60)
                                                                                                                      GTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGGATTTATGATTTCTCCTGCAGGTGTGACGCCACCGCCCTGCGGGGGGGCAAAGTTATTAATCTGATTTACAGCCTGATTATTTTGCGT                       >  SRR19973066.3553854/1‑150 (MQ=60)
                                                                                                                            AGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCGTTAATGAGTGATGGCCTTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGGTATTAAGCTGATTTACAGCCAGATTATTGTGGGTGAGGCG                 >  SRR19973066.2904778/1‑150 (MQ=60)
                                                                                                                                    CGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACAT         <  SRR19973066.3318151/150‑1 (MQ=60)
                                                                                                                                    CGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACGGAGTGTTGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGGGGGGGCACAGTTATTAAGCTGATTTTAAGCCAGATTATTTTGCGTGTGGCGGCGCccaa         >  SRR19973066.2698854/1‑146 (MQ=60)
                                                                                                                                      CAACTAACGCTTCATTAACCAGGGCCGCTTGTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGGGTGACGCCGCCCCCCAGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCGGATTATTTTTCGTGAGGCGGCGCACAAAA       >  SRR19973066.2298901/1‑150 (MQ=60)
                                                                                                                                      CAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAA       <  SRR19973066.1774107/150‑1 (MQ=60)
                                                                                                                                      CAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAA       <  SRR19973066.1618880/150‑1 (MQ=60)
                                                                                                                                      CAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAA       <  SRR19973066.1774020/150‑1 (MQ=60)
                                                                                                                                      CAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAA       <  SRR19973066.3397919/150‑1 (MQ=60)
                                                                                                                                       AACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAAT      <  SRR19973066.618396/150‑1 (MQ=60)
                                                                                                                                         CTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCC    <  SRR19973066.1696723/150‑1 (MQ=60)
                                                                                                                                         CTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCC    <  SRR19973066.1696503/150‑1 (MQ=60)
                                                                                                                                           AACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAG  <  SRR19973066.1249454/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                           
CATCAAACTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAG  >  CP009273/2236949‑2237237

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑