Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F86 I0 R1 593 297.4 9515568 94.4% 8982696 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,237,098 G→T 88.0% H12N (CAT→AAT)  folE ← GTP cyclohydrolase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,237,0980GT88.0% 597.9 / 75.9 218H12N (CAT→AAT) folEGTP cyclohydrolase I
Reads supporting (aligned to +/- strand):  ref base G (16/10);  new base T (79/113);  total (95/123)
Fisher's exact test for biased strand distribution p-value = 5.88e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.94e-01

Only 100 of 219 total aligned reads displayed.

CAAACTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGC‑GTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGC  >  CP009273/2236952‑2237247
                                                                                                                                                  |                                                                                                                                                      
cAAACTGTCATCAGCCAGGTCGAGATTCGGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAAc                                                                                                                                                     >  2:496069/1‑150 (MQ=255)
cAAACTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAAc                                                                                                                                                     <  2:3061732/150‑1 (MQ=255)
 aaaCTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAAcc                                                                                                                                                    <  1:4692880/150‑1 (MQ=255)
 aaaCCGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAAcc                                                                                                                                                    <  2:27560/150‑1 (MQ=255)
   aCTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAg                                                                                                                                                  <  1:68053/150‑1 (MQ=255)
   aCTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAg                                                                                                                                                  <  1:181861/150‑1 (MQ=255)
   aCTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAg                                                                                                                                                  <  1:181193/150‑1 (MQ=255)
   aCTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAg                                                                                                                                                  <  1:636145/150‑1 (MQ=255)
    cTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAgg                                                                                                                                                 <  1:1429422/150‑1 (MQ=255)
     tGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAggg                                                                                                                                                >  1:2257194/1‑150 (MQ=255)
      gTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGc                                                                                                                                               <  1:738609/150‑1 (MQ=255)
      gTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGc                                                                                                                                               >  1:3871687/1‑150 (MQ=255)
             gCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGcttct                                                                                                                                        <  2:3366130/150‑1 (MQ=255)
              ccAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGcttctt                                                                                                                                       >  2:1061373/1‑150 (MQ=255)
                aGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTa                                                                                                                                     <  2:4163192/150‑1 (MQ=255)
                 ggTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTAc                                                                                                                                    >  2:4255066/1‑150 (MQ=255)
                   tCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTg                                                                                                                                  <  1:1130719/150‑1 (MQ=255)
                     gagaTTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTGGTTACagag                                                                                                                                >  1:2837790/1‑150 (MQ=255)
                      agaTTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGt                                                                                                                               >  2:1659062/1‑150 (MQ=255)
                      agaTTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGt                                                                                                                               <  1:4130204/150‑1 (MQ=255)
                      agaTTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTAATGAGt                                                                                                                               >  1:2841984/1‑150 (MQ=255)
                        aTTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGTGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGa                                                                                                                             >  2:4583873/1‑150 (MQ=255)
                        aTTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGTGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGa                                                                                                                             >  2:2201287/1‑150 (MQ=255)
                        aTTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTGTTTACTGAGTGa                                                                                                                             >  1:3679378/1‑150 (MQ=255)
                        aTTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCAAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTAATGATTGa                                                                                                                             >  2:1935091/1‑150 (MQ=255)
                          tCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATg                                                                                                                           <  2:1207230/150‑1 (MQ=255)
                           cagcagCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATgg                                                                                                                          <  1:2365565/150‑1 (MQ=255)
                           cagcagCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATgg                                                                                                                          <  1:3518219/150‑1 (MQ=255)
                            agcagcTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCGTCTTTACTGAGTGTTGGc                                                                                                                         >  1:2540303/1‑150 (MQ=255)
                            agcagcTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGc                                                                                                                         <  1:1000737/150‑1 (MQ=255)
                                       gATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGAttt                                                                                                              <  1:3213998/150‑1 (MQ=255)
                                       gATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCGTGTTTACTTAGTGTTTGCGTTTTTGAttt                                                                                                              >  1:2449808/1‑150 (MQ=255)
                                       gATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCGTCTTTAATTATTGATGGCATTTTTTAttt                                                                                                              >  1:3347859/1‑150 (MQ=255)
                                       gATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGAttt                                                                                                              <  2:1793494/150‑1 (MQ=255)
                                        aTTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGAttta                                                                                                             >  2:3144063/1‑149 (MQ=255)
                                        aTTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCGTGTTTACTGAGTGATTGCAGTTAtttttta                                                                                                             >  1:1982147/1‑144 (MQ=255)
                                        aTTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCGTTACTGAGTGATGGCCTTTATGATTtc                                                                                                             >  1:1459945/1‑150 (MQ=255)
                                         tttCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTtct                                                                                                            <  1:432600/150‑1 (MQ=255)
                                            cGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCt                                                                                                         <  2:1050931/150‑1 (MQ=255)
                                             ggTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTg                                                                                                        <  2:3686667/150‑1 (MQ=255)
                                               tCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCa                                                                                                      >  1:1127108/1‑150 (MQ=255)
                                               tCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGTGTGATGGGATTTTTTATTTATCCTGCa                                                                                                      >  1:1466872/1‑150 (MQ=255)
                                                cATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAg                                                                                                     >  2:245616/1‑150 (MQ=255)
                                                 atatGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCGTCTTTTAAGAGTGATTGCCGTTTTTATTTGTCCAGCAgg                                                                                                    >  1:3177582/1‑150 (MQ=255)
                                                    tGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTTCTGAGTGATTGGATTTATTATTTATCCTGCCGgggt                                                                                                 >  2:4027370/1‑150 (MQ=255)
                                                    tGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGgtgt                                                                                                 >  2:1143310/1‑150 (MQ=255)
                                                     gACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGtgtg                                                                                                <  2:4502546/150‑1 (MQ=255)
                                                         aGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTATTTAATGATTGATGGCATTTATGTTTTATCCTGCAGGTGTGACGc                                                                                            >  1:2358220/1‑150 (MQ=255)
                                                           cAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCGTTACTGAGTGATGGCAAGTATGATTTCTCCGGCAGGTGTGTCGCCt                                                                                          >  2:1394157/1‑150 (MQ=255)
                                                           cAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCGTCTTTTCTGAGTGATTGGATTTTTTATTTCTACGGCAGGGGTGTCGccg                                                                                          >  1:3632362/1‑149 (MQ=255)
                                                             aTAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTcc                                                                                        <  2:1236422/150‑1 (MQ=255)
                                                             aTAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTcc                                                                                        <  2:4540828/150‑1 (MQ=255)
                                                              tAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCg                                                                                       <  2:4256941/150‑1 (MQ=255)
                                                                 ggCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATTGGATTTATTATTTATCCAGCAGGTGTGTCGCCTCCGccc                                                                                    >  1:603388/1‑150 (MQ=255)
                                                                  gCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCt                                                                                   <  1:963425/150‑1 (MQ=255)
                                                                  gCTTTTGCGCGTTTCGTTATCCATGTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCt                                                                                   <  2:4073174/150‑1 (MQ=255)
                                                                  gCTTGTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCt                                                                                   <  2:2598236/150‑1 (MQ=255)
                                                                   cTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTTATGGCATTTATGATTTATACAGCAGGTGTGAAGCCTccccccag                                                                                  >  1:4455248/1‑148 (MQ=255)
                                                                   cTTGTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTg                                                                                  <  2:3410532/150‑1 (MQ=255)
                                                                       tGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGC‑GTg                                                                             >  2:3905265/1‑150 (MQ=255)
                                                                        gcgcGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGC‑GTgg                                                                            <  2:1148374/150‑1 (MQ=255)
                                                                        gcgcGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGC‑GTgg                                                                            <  2:782459/150‑1 (MQ=255)
                                                                         cgcgTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGC‑GTggg                                                                           <  2:3120560/150‑1 (MQ=255)
                                                                          gcgTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTGTTTAAAGATTGATTGGCTGTATGTTTTATACATCAGGGGTGAAGCCTCCGCCCAGC‑GTgggg                                                                          >  1:1029562/1‑150 (MQ=255)
                                                                              ttCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGAGCAAATAACGCTTCATTAAACAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGC‑GTGGGGGCaa                                                                      <  1:1935091/150‑1 (MQ=255)
                                                                                   tATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGC‑GTGGGGGCAAAGtta                                                                 >  2:282437/1‑150 (MQ=255)
                                                                                   tATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTAATGAGTGTTGGCAGTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCAGC‑GTGGGGGCAAAGtta                                                                 >  1:1883188/1‑150 (MQ=255)
                                                                                    aTCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGC‑GTGGGGGCAAAGttat                                                                <  2:3520281/150‑1 (MQ=255)
                                                                                    aTCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGC‑GTGGGGGCAAAGttat                                                                <  2:1639216/150‑1 (MQ=255)
                                                                                     tCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGC‑GTGGGGGCAAAGttatt                                                               <  1:1659062/150‑1 (MQ=255)
                                                                                        aTTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTGTTTACTGAGTGGTGGGATTTATGATTTGTGCTGCAGGTGTGACGCCTCCGCCCTGC‑GTGGGGGCAAAGTTATTaat                                                            >  2:442610/1‑149 (MQ=255)
                                                                                        aTTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGC‑GTGGGGGCAAAGTTATTAAg                                                            <  2:232279/150‑1 (MQ=255)
                                                                                         tttCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGC‑GTGGGGGCAAAGTTATTAAGc                                                           <  2:1708344/150‑1 (MQ=255)
                                                                                               gCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGC‑GTGGGGGCAAAGTTATTAAGCTGAttt                                                     <  2:2257194/150‑1 (MQ=255)
                                                                                                cACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGC‑GTGGGGGCAAAGTTATTAAGCTGATTTa                                                    <  2:1640078/150‑1 (MQ=255)
                                                                                                 aCGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGC‑GTGGGGGCAAAGTTATTAAGCTGATTTAc                                                   <  1:124955/150‑1 (MQ=255)
                                                                                                 aCGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCGTGTTTAATTAGTTTTGGGAGTTTTGATTTATCCTTCAGGGGTGTCGCCGCCCCCCAGC‑GGGGGGGGGCAGGTATTAAACAGAGTTAc                                                   >  1:1600206/1‑150 (MQ=255)
                                                                                                    ggcgggcgCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCGTGGTTAAAGATTGGAGGCCGTTATTTTTTCTCCTTCAGGGGTGACGCCACCGCCCCGCGGTGGGGCCAAAG‑TAATAAACAGAATTACAGc                                                >  1:4214247/1‑150 (MQ=255)
                                                                                                      cgggcgCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGC‑GTGGGGGCAAAGTTATTAAGCTGATTTACAGCCt                                              <  2:945600/150‑1 (MQ=255)
                                                                                                       gggcgCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTATCCTGCAGGTGTGACGCCTCCGCCCTGC‑GTGGGGGCCAAGTTATTAAGCTGATTTACAGCCTg                                             >  1:3926141/1‑150 (MQ=255)
                                                                                                       gggcgCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGC‑GTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTg                                             <  2:3268572/150‑1 (MQ=255)
                                                                                                        ggcgCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGC‑GTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGa                                            <  1:883780/150‑1 (MQ=255)
                                                                                                           gcAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGC‑GTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGatta                                         <  1:496069/150‑1 (MQ=255)
                                                                                                           gcAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGC‑GTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGatta                                         <  2:3736168/150‑1 (MQ=255)
                                                                                                              gCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGC‑GTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATtg                                      <  2:541572/150‑1 (MQ=255)
                                                                                                              gCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCGTGTTTACAGAGTGATGGCATTTATGATTTATACAGCAGGTGTGACGCCGCCGCCCAGC‑CGGGGGGCCAAGTTAATAATCAGATTTACAGCCAGattattt                                      >  1:2887184/1‑149 (MQ=255)
                                                                                                                  tgtTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGGTGGGATTTATTATTTATCCTTCAGGTGTGTCGCCACCGCCCAGC‑GTGGGGGCAAAGTTATTAATCTGATTTACAGCCTGattatttttcg                                  >  1:1046049/1‑145 (MQ=255)
                                                                                                                   gtTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCAGC‑GTGGGGGCACAGTTATTAAGCTGATTTACAGCCTGATTATTTTGCGt                                 >  2:286664/1‑150 (MQ=255)
                                                                                                                    tttCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGTTGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGC‑GTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTg                                >  2:3368632/1‑150 (MQ=255)
                                                                                                                      tCCAGTCCTCGCGCACCTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGC‑GTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAg                              <  2:2567926/150‑1 (MQ=255)
                                                                                                                         aGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGGGTTGGCATTTATGTTTTGTCCGGCAGGGGTGTCGCCTACCCCCGGC‑GGGGGGGCAAAGTTATTAAGAGGTTTTACAGCCAGATTATTTTTCGTGAggcg                           >  2:1988776/1‑150 (MQ=255)
                                                                                                                         aGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTAATGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCAGC‑GTGGGGGCAAAGTTATTAAGCGGATTTAAAGCCTGATTATTTTGAGTGAggcg                           >  2:4374108/1‑150 (MQ=255)
                                                                                                                                   cAACTAACGCTTCATGAACCAGGGCCGCTTGTTTACTGATTGATGGGATTTATGATTTCTCCTGCAGGTGTGACGCCACCGCCCAGC‑GTGGGGGCAAAGTTATTAATCAGATTTAAAGCCAGATTATTTTTGGTGAGGCGGCGCACaaaa                 >  2:820131/1‑150 (MQ=255)
                                                                                                                                   cAACTAACGCTTCATGAACCAGGGCCGCGTGTTTAAAGAGTGGTGGCATTTATGATTTCTCCAGCCGGTGTGTCGCCGCCCCCCTGC‑GGGGGGGCAAACTTAATAAGATGATTTACAGCCTGAATATTTTGCGTGAGGCGGCGCACaaaa                 >  2:3996843/1‑150 (MQ=255)
                                                                                                                                    aaCTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGTTGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGC‑GTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGAGTGAGGCGGCGCACATAAt                >  2:3545653/1‑150 (MQ=255)
                                                                                                                                      cTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGC‑GTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATcc              <  1:4583744/150‑1 (MQ=255)
                                                                                                                                      cTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGC‑GTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATcc              <  2:4703533/150‑1 (MQ=255)
                                                                                                                                         aCGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGC‑GTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGt           <  1:2512609/150‑1 (MQ=255)
                                                                                                                                          cGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGC‑GTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTa          <  2:3650403/150‑1 (MQ=255)
                                                                                                                                                  gAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGC‑GTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGc  <  2:1986338/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                      
CAAACTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGC‑GTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGC  >  CP009273/2236952‑2237247

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATCAAACTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGC  >  CP009273/2236950‑2237247
                                                                                                                                                    |                                                                                                                                                     
ATCAAACTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTA                                                                                                                                                      >  SRR19973082.754/1‑150 (MQ=60)
  CAAACTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAAC                                                                                                                                                    >  SRR19973082.4194922/1‑150 (MQ=60)
   AAACTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACC                                                                                                                                                   <  SRR19973082.4721162/150‑1 (MQ=60)
   AAACTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACC                                                                                                                                                   <  SRR19973082.1727173/150‑1 (MQ=60)
    AACTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCA                                                                                                                                                  <  SRR19973082.2264570/150‑1 (MQ=60)
    AACTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCA                                                                                                                                                  >  SRR19973082.234113/1‑150 (MQ=60)
     ACTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAG                                                                                                                                                 <  SRR19973082.183401/150‑1 (MQ=60)
     ACTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAG                                                                                                                                                 <  SRR19973082.182728/150‑1 (MQ=60)
     ACTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAG                                                                                                                                                 <  SRR19973082.68792/150‑1 (MQ=60)
     ACTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAG                                                                                                                                                 <  SRR19973082.68789/150‑1 (MQ=60)
     ACTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAG                                                                                                                                                 <  SRR19973082.640577/150‑1 (MQ=60)
      CTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGG                                                                                                                                                <  SRR19973082.1439171/150‑1 (MQ=60)
       TGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGG                                                                                                                                               >  SRR19973082.2271361/1‑150 (MQ=60)
       TGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGG                                                                                                                                               <  SRR19973082.2748702/150‑1 (MQ=60)
        GTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGC                                                                                                                                              <  SRR19973082.743469/150‑1 (MQ=60)
        GTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGC                                                                                                                                              >  SRR19973082.3895428/1‑150 (MQ=60)
             CAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTT                                                                                                                                         <  SRR19973082.3902505/150‑1 (MQ=60)
                CCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTT                                                                                                                                      >  SRR19973082.3672899/1‑150 (MQ=60)
                CCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTT                                                                                                                                      >  SRR19973082.3112491/1‑150 (MQ=60)
                CCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTagt                                                                                                                                      >  SRR19973082.4005858/1‑147 (MQ=60)
                  AGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTA                                                                                                                                    <  SRR19973082.1352266/150‑1 (MQ=60)
                   GGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCgtgtgtaa                                                                                                                                   >  SRR19973082.3229384/1‑142 (MQ=60)
                     TCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTG                                                                                                                                 <  SRR19973082.1138256/150‑1 (MQ=60)
                     TCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTG                                                                                                                                 <  SRR19973082.1137977/150‑1 (MQ=60)
                      CGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACAGA                                                                                                                                >  SRR19973082.630445/1‑150 (MQ=60)
                       GAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTggttacagag                                                                                                                               >  SRR19973082.2855207/1‑140 (MQ=60)
                        AGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGT                                                                                                                              <  SRR19973082.4155497/150‑1 (MQ=60)
                        AGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTAATGAGT                                                                                                                              >  SRR19973082.1933726/1‑150 (MQ=60)
                        AGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCGTCTTTACAGAGT                                                                                                                              >  SRR19973082.2859416/1‑150 (MQ=60)
                        AGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCGTATTTAATGAGT                                                                                                                              >  SRR19973082.2859432/1‑150 (MQ=60)
                          ATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTGTTTACTGAGTGA                                                                                                                            >  SRR19973082.3702125/1‑150 (MQ=60)
                             CAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGG                                                                                                                         <  SRR19973082.3539678/150‑1 (MQ=60)
                             CAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGG                                                                                                                         <  SRR19973082.2380104/150‑1 (MQ=60)
                              AGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCGTCTTTACTGAGTGTTGGC                                                                                                                        >  SRR19973082.2556158/1‑150 (MQ=60)
                              AGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGC                                                                                                                        <  SRR19973082.1007706/150‑1 (MQ=60)
                              AGCAGCTGCATGATTTCGGTCATATGAACAGCAATAAGGCTATTGCGCGTTTCGTTATCCATTACATGCACTGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCACCTAACGCTACATCAACCAGGGCCGCTTattaaaagactgattgg                                                                                                                        >  SRR19973082.3350868/1‑133 (MQ=60)
                                 AGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATT                                                                                                                     <  SRR19973082.1543741/150‑1 (MQ=60)
                                         GATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCgtgtttacttagtgtttgcgtttttgattt                                                                                                             >  SRR19973082.2465064/1‑120 (MQ=60)
                                         GATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTGTTTACTTAGTTTTGGGATTTATGATTT                                                                                                             >  SRR19973082.977610/1‑150 (MQ=60)
                                         GATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTT                                                                                                             <  SRR19973082.3233628/150‑1 (MQ=60)
                                         GATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTT                                                                                                             <  SRR19973082.3233645/150‑1 (MQ=60)
                                         GATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCGTCTTTAATTATTGATGGCATTTTTTATTT                                                                                                             >  SRR19973082.3368561/1‑150 (MQ=60)
                                          ATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCGTGTTTACTGAGTGATtgcagttatttttta                                                                                                            >  SRR19973082.1994557/1‑135 (MQ=60)
                                          ATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCGTTACTGAGTGATGGCCTTTATGATTTC                                                                                                            >  SRR19973082.1469800/1‑150 (MQ=60)
                                           TTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCT                                                                                                           <  SRR19973082.435542/150‑1 (MQ=60)
                                             TCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCGTTAATGAGTGATGGCATGTATGATTTATCC                                                                                                         >  SRR19973082.3318446/1‑150 (MQ=60)
                                              CGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCT                                                                                                        <  SRR19973082.2426884/150‑1 (MQ=60)
                                               GGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTG                                                                                                       <  SRR19973082.1118881/150‑1 (MQ=60)
                                               GGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCGTCTTTTATGAGGGATGGCAGTTATGATTTCTCCCG                                                                                                       >  SRR19973082.4539390/1‑150 (MQ=60)
                                                 TCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCA                                                                                                     >  SRR19973082.1134631/1‑150 (MQ=60)
                                                 TCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGTGTGATGGGATTTTTTATTTATCCTGCA                                                                                                     >  SRR19973082.1476758/1‑150 (MQ=60)
                                                  CATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCGTCTTTAATGAGTGATGGCCTTTATGATTTCTCCTGCAG                                                                                                    >  SRR19973082.4744298/1‑150 (MQ=60)
                                                   ATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGG                                                                                                   <  SRR19973082.182863/150‑1 (MQ=60)
                                                   ATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCGTCTTTtaagagtgattgccgtttttatttgtccagcagg                                                                                                   >  SRR19973082.3196783/1‑116 (MQ=60)
                                                       GACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCGTCTTTACTGAGTGATGGCATTTATGATTTATCCTGCAGGTGTG                                                                                               >  SRR19973082.742732/1‑150 (MQ=60)
                                                         CCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCAGCAGGTGTGAC                                                                                             >  SRR19973082.322676/1‑150 (MQ=60)
                                                          CAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGTCG                                                                                            >  SRR19973082.1966899/1‑150 (MQ=60)
                                                          CAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACG                                                                                            <  SRR19973082.1016912/150‑1 (MQ=60)
                                                           AGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCCTTTATGTTTTCTCCTGCAGGTGTGACGC                                                                                           >  SRR19973082.2372737/1‑150 (MQ=60)
                                                           AGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTATTTAATGATTGATGGCATTTATGTTTTATCCTGCAGGTGTGACGC                                                                                           >  SRR19973082.2372724/1‑150 (MQ=60)
                                                             CAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTgggttaattagtgatggcctgttttttttatacaccacgtgtgtccaca                                                                                         >  SRR19973082.3202862/1‑101 (MQ=60)
                                                             CAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCGTCTTTTCTGAGTGATtggattttttatttctacggcaggggtgtcgccg                                                                                         >  SRR19973082.3654697/1‑116 (MQ=60)
                                                               ATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTAatttgtgttgggattttttatttataaagcaggtgtgtagccaac                                                                                       >  SRR19973082.1283153/1‑105 (MQ=60)
                                                                   GGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATTGGATTTATTATTTATCCAGCAGGTGTGTCGCCTCCGCCC                                                                                   >  SRR19973082.607676/1‑150 (MQ=60)
                                                                    GCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTTCTGTGTGTTTGCATTTATTATTTGTCCTGCAGGTGTGtaggctccgccca                                                                                  >  SRR19973082.2843175/1‑137 (MQ=60)
                                                                    GCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCT                                                                                  <  SRR19973082.970130/150‑1 (MQ=60)
                                                                     CTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTTATGGCATTTATGATTTATACAGCAGGTGTGAAGCCTCCCCCCAG                                                                                 >  SRR19973082.4482316/1‑150 (MQ=60)
                                                                            GCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTgtttaaagattgattggctgtatgttttatacatcaggggtgaagcctccgcccagcgtgggg                                                                          >  SRR19973082.1036687/1‑87 (MQ=60)
                                                                              GTTTCGTTATCCATTTCATGCACGGGCGGGCACAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGC                                                                        >  SRR19973082.3574919/1‑150 (MQ=60)
                                                                              GTTTCGTTATCCATTTCATGCACGGGCGGGCACAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGC                                                                        >  SRR19973082.1163617/1‑150 (MQ=60)
                                                                               TTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCA                                                                       <  SRR19973082.4626695/150‑1 (MQ=60)
                                                                                TTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGAGCAAATAACGCTTCATTAAACAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAA                                                                      <  SRR19973082.1947356/150‑1 (MQ=60)
                                                                                    TTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCGTGTTTAATGAGTGGTGGCATTTATGATTTCTCCAGCCGGTGTGTCGCCTCCGCCCAGCGTGGGGGCCAAGGT                                                                  >  SRR19973082.2532349/1‑150 (MQ=60)
                                                                                     TATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTA                                                                 >  SRR19973082.4292170/1‑150 (MQ=60)
                                                                                     TATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTAATGAGTGTTGGCAGTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCAGCGTGGGGGCAAAGTTA                                                                 >  SRR19973082.1895301/1‑150 (MQ=60)
                                                                                       TCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATT                                                               <  SRR19973082.1670108/150‑1 (MQ=60)
                                                                                       TCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATT                                                               <  SRR19973082.1451207/150‑1 (MQ=60)
                                                                                       TCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATT                                                               <  SRR19973082.1474198/150‑1 (MQ=60)
                                                                                       TCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACAGAGTGATTGGATTTATGATTTCTACAGCAGGGGTGAAGCCGCCGCCCAGCGTGGGGGCAAAGTTATT                                                               >  SRR19973082.2509487/1‑150 (MQ=60)
                                                                                       TCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATT                                                               <  SRR19973082.790480/150‑1 (MQ=60)
                                                                                               ATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGAT                                                       <  SRR19973082.4366286/150‑1 (MQ=60)
                                                                                               ATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGAT                                                       <  SRR19973082.3370141/150‑1 (MQ=60)
                                                                                                TGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATT                                                      <  SRR19973082.4521551/150‑1 (MQ=60)
                                                                                                   ACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCgtgtttaattagttttgggagttttgatttatccttcaggggtgtcgccgccccccagcgggggggggcaggtattaaacagagttac                                                   >  SRR19973082.1610754/1‑62 (MQ=60)
                                                                                                   ACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTAC                                                   <  SRR19973082.126090/150‑1 (MQ=60)
                                                                                                      GGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCgtggttaaagattggaggccgttattttttctccttcaggggtgacgccaccgccccgcggtggggccaaagtaataaacagaattacagc                                                >  SRR19973082.4239944/1‑59 (MQ=60)
                                                                                                      GGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGCTGGCATTTATGATTTCTCCAGCAGGTGTGACGCCACCCCCCAGCGTGGGGGCCAAGTTATTAAGCTGATTTACAGC                                                >  SRR19973082.1843100/1‑150 (MQ=60)
                                                                                                      GGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGC                                                <  SRR19973082.176608/150‑1 (MQ=60)
                                                                                                         GGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTTCTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTAAAGCCTG                                             >  SRR19973082.3949902/1‑150 (MQ=60)
                                                                                                         GGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTATCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCCAAGTTATTAAGCTGATTTACAGCCTG                                             >  SRR19973082.3950067/1‑150 (MQ=60)
                                                                                                         GGGCGCAGCGGTGTTTCCAGTCCTCGCGAAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTG                                             <  SRR19973082.95162/150‑1 (MQ=60)
                                                                                                          GGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGA                                            <  SRR19973082.889854/150‑1 (MQ=60)
                                                                                                          GGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGA                                            <  SRR19973082.738599/150‑1 (MQ=60)
                                                                                                             GCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTA                                         <  SRR19973082.499614/150‑1 (MQ=60)
                                                                                                             GCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTA                                         <  SRR19973082.499601/150‑1 (MQ=60)
                                                                                                                GCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCGTGTTTACAGAGTGATGGCATTTATGATTTATACAGCAGGTGTGACGCCGCCGCCCAGCCGGGGGGCCAAGTTAATAATCAGATTTACAGCCAGATTATTT                                      >  SRR19973082.2905008/1‑150 (MQ=60)
                                                                                                                    TGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTGTTTAATGAGTGATTGCATTTATTATTTATCCTGCAGGGGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTTATCTGATTTAaatccagaatatttttcg                                  >  SRR19973082.1053240/1‑132 (MQ=60)
                                                                                                                    TGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGGTGGGATTTATTATTTATCCTTCAGGTGTGTCGCCACCGCCCAGCGTGGGGGCAAAGTTATTAATCTGATTTACAGCCTGATTATTtttcg                                  >  SRR19973082.1053267/1‑145 (MQ=60)
                                                                                                                       TTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGA                               <  SRR19973082.1416473/150‑1 (MQ=60)
                                                                                                                                 CGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCAC                     <  SRR19973082.2902561/150‑1 (MQ=60)
                                                                                                                                     CAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAA                 <  SRR19973082.2257202/150‑1 (MQ=60)
                                                                                                                                        CTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCC              <  SRR19973082.4611574/150‑1 (MQ=60)
                                                                                                                                         TAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCA             <  SRR19973082.3710671/150‑1 (MQ=60)
                                                                                                                                           ACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGT           <  SRR19973082.2528291/150‑1 (MQ=60)
                                                                                                                                              CTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATT        <  SRR19973082.1660901/150‑1 (MQ=60)
                                                                                                                                                  ATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGGTTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACA    <  SRR19973082.1770945/150‑1 (MQ=60)
                                                                                                                                                    TAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGC  <  SRR19973082.1902672/150‑1 (MQ=60)
                                                                                                                                                    TAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGC  <  SRR19973082.4298960/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
ATCAAACTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGC  >  CP009273/2236950‑2237247

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑