Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,265,064 C→A 100% intergenic (‑95/‑335) yjcB ← / → yjcC putative inner membrane protein/putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,265,0640CA97.1% 784.5 / 13.3 242intergenic (‑95/‑335)yjcB/yjcCputative inner membrane protein/putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base C (6/1);  new base A (103/132);  total (109/133)
Fisher's exact test for biased strand distribution p-value = 4.79e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.97e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 247 total aligned reads displayed.

GCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCT  >  CP009273/4264917‑4265213
                                                                                                                                                   |                                                                                                                                                     
gCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAaaac                                                                                                                                                     <  2:1935734/150‑1 (MQ=255)
  aCTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACaa                                                                                                                                                   <  1:3176400/150‑1 (MQ=255)
    tAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACaaaa                                                                                                                                                 >  1:1548748/1‑150 (MQ=255)
    tAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACaaaa                                                                                                                                                 >  1:2170865/1‑150 (MQ=255)
     aagaagTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACaaaaa                                                                                                                                                <  1:2821391/150‑1 (MQ=255)
      agaagTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAAt                                                                                                                                               <  2:669568/150‑1 (MQ=255)
       gaagTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATg                                                                                                                                              >  1:2298167/1‑150 (MQ=255)
        aagTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGa                                                                                                                                             >  2:363792/1‑150 (MQ=255)
         agTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGaa                                                                                                                                            >  2:2838458/1‑150 (MQ=255)
          gTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGaaa                                                                                                                                           >  1:573408/1‑150 (MQ=255)
          gTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGaaa                                                                                                                                           >  2:3056728/1‑150 (MQ=255)
              ccAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACAct                                                                                                                                       >  1:589598/1‑150 (MQ=255)
                 gCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCtg                                                                                                                                    <  1:985323/150‑1 (MQ=255)
                   gaagaagaaCCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCtgtt                                                                                                                                  >  2:2243768/1‑150 (MQ=255)
                    aagaagaaCCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCtgttt                                                                                                                                 >  1:3808598/1‑150 (MQ=255)
                    aagaagaaCCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCtgttt                                                                                                                                 <  1:1464781/150‑1 (MQ=255)
                       aagaaCCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCtgtttgtt                                                                                                                              >  1:4006904/1‑150 (MQ=255)
                        agaaCCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTgtttgttt                                                                                                                             >  2:3884753/1‑150 (MQ=255)
                        agaaCCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTgtttgttt                                                                                                                             <  2:3377927/150‑1 (MQ=255)
                        agaaCCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTgtttgttt                                                                                                                             <  1:3459216/150‑1 (MQ=255)
                         gaaCCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTc                                                                                                                            >  2:2151920/1‑150 (MQ=255)
                          aaCCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCa                                                                                                                           <  2:2954257/150‑1 (MQ=255)
                          aaCCACGCCAGTGGGGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGGACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCa                                                                                                                           >  2:2736674/1‑150 (MQ=255)
                           aCCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCAt                                                                                                                          <  1:4307532/150‑1 (MQ=255)
                            ccACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGGTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCAtt                                                                                                                         <  2:3922313/150‑1 (MQ=255)
                               cGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAAt                                                                                                                      <  2:2422674/150‑1 (MQ=255)
                                gCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCTCGGAGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTACATTAAtt                                                                                                                     >  2:3749600/1‑150 (MQ=255)
                                   aGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTtg                                                                                                                  >  2:1478932/1‑150 (MQ=255)
                                     tggtgAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTtgtg                                                                                                                >  2:143131/1‑150 (MQ=255)
                                       gtgAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGaa                                                                                                              >  1:677090/1‑150 (MQ=255)
                                        tgAGGGTATCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAAc                                                                                                             <  1:645485/150‑1 (MQ=255)
                                         gAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACt                                                                                                            <  2:4005259/150‑1 (MQ=255)
                                         gAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACt                                                                                                            <  1:1343212/150‑1 (MQ=255)
                                          aGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACta                                                                                                           <  2:4396024/150‑1 (MQ=255)
                                          aGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACta                                                                                                           <  1:4327192/150‑1 (MQ=255)
                                             gTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTatat                                                                                                        <  2:1381386/150‑1 (MQ=255)
                                              tAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATc                                                                                                       <  2:3829247/150‑1 (MQ=255)
                                              tAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATc                                                                                                       >  1:1340353/1‑150 (MQ=255)
                                               aGCCATAAAACAAATCCTCTGAAAACAGCCGAAGCCCTGGACACCGAGGCGAAGATCAAGGGAGGCGAGGTACACTCTGGCTATCGCGGGTCTTGCAGAAAACAAAAATGAAAAACACTTATTGAATCATTAAATTTTTCAACAATATca                                                                                                      >  1:3040462/1‑150 (MQ=255)
                                                  cATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACaa                                                                                                   >  2:4073967/1‑150 (MQ=255)
                                                  cATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACaa                                                                                                   >  1:1835403/1‑150 (MQ=255)
                                                  cATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACaa                                                                                                   >  2:4580106/1‑150 (MQ=255)
                                                     cAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACTattg                                                                                                >  2:1266176/1‑150 (MQ=255)
                                                     cAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAattg                                                                                                <  1:3277192/150‑1 (MQ=255)
                                                       aaCAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAattgat                                                                                              >  2:1854470/1‑150 (MQ=255)
                                                        aCAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAattgatt                                                                                             <  2:891843/150‑1 (MQ=255)
                                                         cAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAattgattg                                                                                            <  1:3071085/150‑1 (MQ=255)
                                                          aaTTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGAttgt                                                                                           <  1:243172/150‑1 (MQ=255)
                                                            ttCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGAttgttt                                                                                         >  2:3857252/1‑150 (MQ=255)
                                                              ccTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGAttgtttgt                                                                                       <  1:748302/150‑1 (MQ=255)
                                                                tctGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTa                                                                                     <  2:2634711/150‑1 (MQ=255)
                                                                 ctGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAg                                                                                    <  2:3369949/150‑1 (MQ=255)
                                                                 ctGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAg                                                                                    <  2:1407611/150‑1 (MQ=255)
                                                                     aaaGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCat                                                                                >  2:3935700/1‑150 (MQ=255)
                                                                      aaGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCata                                                                               <  1:4520937/150‑1 (MQ=255)
                                                                       agagCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCatat                                                                              <  2:2357289/150‑1 (MQ=255)
                                                                       agagCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCatat                                                                              <  2:2357367/150‑1 (MQ=255)
                                                                        gagCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATAtt                                                                             <  1:3516697/150‑1 (MQ=255)
                                                                         agCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTa                                                                            >  2:3272434/1‑150 (MQ=255)
                                                                            cGATGCCCTGGACAGCGCGGCGTTGTTCTCGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACTCTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGc                                                                         <  1:3870307/150‑1 (MQ=255)
                                                                             gATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGcc                                                                        >  1:148374/1‑150 (MQ=255)
                                                                             gATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGcc                                                                        <  2:2173791/150‑1 (MQ=255)
                                                                             gATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTGGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGcc                                                                        >  2:3402864/1‑150 (MQ=255)
                                                                              aTGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCg                                                                       >  1:1884477/1‑150 (MQ=255)
                                                                               tGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGt                                                                      <  1:1214009/150‑1 (MQ=255)
                                                                               tGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGt                                                                      >  1:4210006/1‑150 (MQ=255)
                                                                                    tGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACtt                                                                 >  2:4295361/1‑150 (MQ=255)
                                                                                      gACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACtttt                                                               <  2:2452523/150‑1 (MQ=255)
                                                                                      gACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACtttt                                                               >  2:1316961/1‑150 (MQ=255)
                                                                                       aCAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTa                                                              >  1:3619959/1‑150 (MQ=255)
                                                                                        cAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTAt                                                             >  2:1679512/1‑150 (MQ=255)
                                                                                           cgcgGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGc                                                          >  2:1262826/1‑150 (MQ=255)
                                                                                            gcggcgTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCt                                                         <  2:805471/150‑1 (MQ=255)
                                                                                            gcggcgTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCt                                                         <  1:2841789/150‑1 (MQ=255)
                                                                                                cgTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTAc                                                     <  2:467405/150‑1 (MQ=255)
                                                                                                   tgttCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGa                                                  >  1:3577492/1‑150 (MQ=255)
                                                                                                   tgttCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGa                                                  >  1:2916058/1‑150 (MQ=255)
                                                                                                      tCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCATAAAACAAAAATGAAACACTCTTTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTa                                               <  2:1093758/150‑1 (MQ=255)
                                                                                                      tCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTa                                               <  2:4209703/150‑1 (MQ=255)
                                                                                                       caagggAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATAAGGCCGTGACGTTTATTGCTGTACAGAGTAt                                              >  1:223996/4‑150 (MQ=255)
                                                                                                       cACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTAt                                              >  1:2023366/1‑150 (MQ=255)
                                                                                                           ggAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTgg                                          <  1:2895632/150‑1 (MQ=255)
                                                                                                            gAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGt                                         <  2:2220235/150‑1 (MQ=255)
                                                                                                            gAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGt                                         >  1:1667542/1‑150 (MQ=255)
                                                                                                             aGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGtt                                        <  2:4364727/150‑1 (MQ=255)
                                                                                                             aGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGtt                                        >  2:3464117/1‑150 (MQ=255)
                                                                                                                 gTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTc                                    <  1:2850342/150‑1 (MQ=255)
                                                                                                                 gTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTc                                    <  2:733058/150‑1 (MQ=255)
                                                                                                                     tACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTg                                <  1:4106415/150‑1 (MQ=255)
                                                                                                                     tACACTCTGGCGATCGCGGGGCTTGCAGAAAACTAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTg                                <  2:775040/150‑1 (MQ=255)
                                                                                                                      acacTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTgg                               <  1:4360162/150‑1 (MQ=255)
                                                                                                                        acTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCa                             <  1:3931938/150‑1 (MQ=255)
                                                                                                                         ctctGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCAt                            >  2:59393/1‑150 (MQ=255)
                                                                                                                           ctGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTa                          <  1:3123514/150‑1 (MQ=255)
                                                                                                                            tGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTaa                         <  1:2592941/150‑1 (MQ=255)
                                                                                                                             ggCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAg                        >  2:2550996/1‑150 (MQ=255)
                                                                                                                              gCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAgg                       <  1:3227314/150‑1 (MQ=255)
                                                                                                                                   cgcgGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCata                  <  1:878551/150‑1 (MQ=255)
                                                                                                                                        ggCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTc             <  1:4554492/150‑1 (MQ=255)
                                                                                                                                              cAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCccgccg       >  2:1738310/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
GCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCT  >  CP009273/4264917‑4265213

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCT  >  CP009273/4264915‑4265213
                                                                                                                                                     |                                                                                                                                                     
CGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACTCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAA                                                                                                                                                       >  SRR19973072.3619738/1‑150 (MQ=60)
CGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAA                                                                                                                                                       >  SRR19973072.530506/1‑150 (MQ=60)
CGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAA                                                                                                                                                       >  SRR19973072.2038906/1‑150 (MQ=60)
CGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAA                                                                                                                                                       >  SRR19973072.1796675/1‑150 (MQ=60)
CGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAA                                                                                                                                                       >  SRR19973072.264465/1‑150 (MQ=60)
  GCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAAC                                                                                                                                                     <  SRR19973072.950630/150‑1 (MQ=60)
   CACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACA                                                                                                                                                    <  SRR19973072.4252998/150‑1 (MQ=60)
   CACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACA                                                                                                                                                    <  SRR19973072.1457096/150‑1 (MQ=60)
    ACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAA                                                                                                                                                   <  SRR19973072.3183096/150‑1 (MQ=60)
      TAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAA                                                                                                                                                 >  SRR19973072.1552030/1‑150 (MQ=60)
      TAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAA                                                                                                                                                 >  SRR19973072.2175597/1‑150 (MQ=60)
       AAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAA                                                                                                                                                <  SRR19973072.2827439/150‑1 (MQ=60)
         GAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATG                                                                                                                                              >  SRR19973072.2303081/1‑150 (MQ=60)
           AGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAA                                                                                                                                            >  SRR19973072.3757609/1‑150 (MQ=60)
            GTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAA                                                                                                                                           >  SRR19973072.574747/1‑150 (MQ=60)
              TGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACA                                                                                                                                         >  SRR19973072.57899/1‑150 (MQ=60)
                CCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACT                                                                                                                                       >  SRR19973072.590966/1‑150 (MQ=60)
                CCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACT                                                                                                                                       <  SRR19973072.3182592/150‑1 (MQ=60)
                   GCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTG                                                                                                                                    <  SRR19973072.987475/150‑1 (MQ=60)
                   GCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTG                                                                                                                                    <  SRR19973072.987474/150‑1 (MQ=60)
                   GCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTG                                                                                                                                    <  SRR19973072.190096/150‑1 (MQ=60)
                     GAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTT                                                                                                                                  >  SRR19973072.3528946/1‑150 (MQ=60)
                     GAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTT                                                                                                                                  >  SRR19973072.2178527/1‑150 (MQ=60)
                     GAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTT                                                                                                                                  <  SRR19973072.3107327/150‑1 (MQ=60)
                      AAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTT                                                                                                                                 >  SRR19973072.3816572/1‑150 (MQ=60)
                      AAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTT                                                                                                                                 <  SRR19973072.1467930/150‑1 (MQ=60)
                        GAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGT                                                                                                                               >  SRR19973072.3852335/1‑150 (MQ=60)
                         AAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTT                                                                                                                              >  SRR19973072.4015300/1‑150 (MQ=60)
                          AGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTT                                                                                                                             <  SRR19973072.3466469/150‑1 (MQ=60)
                          AGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTT                                                                                                                             <  SRR19973072.3899263/150‑1 (MQ=60)
                          AGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTT                                                                                                                             <  SRR19973072.3466464/150‑1 (MQ=60)
                          AGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTT                                                                                                                             >  SRR19973072.2071815/1‑150 (MQ=60)
                             ACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCAT                                                                                                                          <  SRR19973072.4316530/150‑1 (MQ=60)
                             ACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCAT                                                                                                                          <  SRR19973072.4316537/150‑1 (MQ=60)
                              CCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATT                                                                                                                         <  SRR19973072.412291/150‑1 (MQ=60)
                               CACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTA                                                                                                                        <  SRR19973072.1828959/150‑1 (MQ=60)
                                     AGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTG                                                                                                                  >  SRR19973072.4550229/1‑150 (MQ=60)
                                        GGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGA                                                                                                               <  SRR19973072.2803904/150‑1 (MQ=60)
                                        GGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGA                                                                                                               <  SRR19973072.2803910/150‑1 (MQ=60)
                                        GGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGA                                                                                                               >  SRR19973072.2405614/1‑150 (MQ=60)
                                         GTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAA                                                                                                              >  SRR19973072.678594/1‑150 (MQ=60)
                                          TGAGGGTATCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAAC                                                                                                             <  SRR19973072.646949/150‑1 (MQ=60)
                                           GAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACT                                                                                                            <  SRR19973072.1346155/150‑1 (MQ=60)
                                           GAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACT                                                                                                            <  SRR19973072.1346156/150‑1 (MQ=60)
                                           GAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCACTAATTTTGTGAACT                                                                                                            <  SRR19973072.642196/150‑1 (MQ=60)
                                            AGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTA                                                                                                           <  SRR19973072.4336241/150‑1 (MQ=60)
                                             GGGTAGCGATCTAACATTTGCTCTGAAAAGATCCGATGCACTGGTCAGCGCGGCGTTGTTCACTGGAGGGGTTGTACACTGTGGCTTTCGCGGGGCTTGGATAAAACAAAAATTAATCACTCTGTTTGTTTCATTAATTTTGTGTACTAT                                                                                                          <  SRR19973072.534217/150‑1 (MQ=53)
                                                TAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATC                                                                                                       >  SRR19973072.3062750/1‑150 (MQ=60)
                                                TAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATC                                                                                                       >  SRR19973072.1343286/1‑150 (MQ=60)
                                                 AGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCA                                                                                                      >  SRR19973072.3311024/1‑150 (MQ=60)
                                                 AGCCATAAAACAAATCCTCTGAAAACAGCCGAAGCCCTGGACACCGAGGCGAAGATCAAGGGAGGCGAGGTACACTCTGGCTATCGCGGGTCTTGCAGAAAACAAAAATGAAAaacacttattgaatcattaaatttttcaacaatatca                                                                                                      >  SRR19973072.3046901/1‑113 (MQ=43)
                                                    CATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAA                                                                                                   >  SRR19973072.1839391/1‑150 (MQ=60)
                                                       CAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTG                                                                                                <  SRR19973072.3284149/150‑1 (MQ=60)
                                                       CAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTG                                                                                                <  SRR19973072.3283860/150‑1 (MQ=60)
                                                       CAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTG                                                                                                >  SRR19973072.1857533/1‑150 (MQ=60)
                                                       CAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTG                                                                                                <  SRR19973072.445981/150‑1 (MQ=60)
                                                        AAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGA                                                                                               >  SRR19973072.3102225/1‑150 (MQ=60)
                                                          ACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATT                                                                                             <  SRR19973072.4147345/150‑1 (MQ=60)
                                                          ACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATT                                                                                             <  SRR19973072.604731/150‑1 (MQ=60)
                                                           CAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTG                                                                                            <  SRR19973072.3077573/150‑1 (MQ=60)
                                                            AATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGT                                                                                           <  SRR19973072.243761/150‑1 (MQ=60)
                                                              TTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTT                                                                                         <  SRR19973072.2405353/150‑1 (MQ=60)
                                                                CCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGT                                                                                       <  SRR19973072.749899/150‑1 (MQ=60)
                                                                   CTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAG                                                                                    >  SRR19973072.4598622/1‑150 (MQ=60)
                                                                       AAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCAT                                                                                <  SRR19973072.2844533/150‑1 (MQ=60)
                                                                       AAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCAG                                                                                >  SRR19973072.1519154/1‑150 (MQ=60)
                                                                        AAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATA                                                                               <  SRR19973072.4530480/150‑1 (MQ=60)
                                                                         AGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACACTTGATTGTTTGTTAGCCAGAT                                                                              >  SRR19973072.2465529/1‑150 (MQ=60)
                                                                          GAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATT                                                                             <  SRR19973072.3524073/150‑1 (MQ=60)
                                                                          GAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATT                                                                             <  SRR19973072.3524081/150‑1 (MQ=60)
                                                                           AGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTA                                                                            <  SRR19973072.2880829/150‑1 (MQ=60)
                                                                            GCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGACACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAG                                                                           <  SRR19973072.4504066/150‑1 (MQ=60)
                                                                              CGATGCCCTGGACAGCGCGGCGTTGTTCTCGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACTCTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGC                                                                         <  SRR19973072.3878351/150‑1 (MQ=60)
                                                                               GATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTTTTTGTTAGCAATATTATGCC                                                                        >  SRR19973072.148715/1‑150 (MQ=60)
                                                                               GATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCC                                                                        >  SRR19973072.3185192/1‑150 (MQ=60)
                                                                               GATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCC                                                                        >  SRR19973072.148752/1‑150 (MQ=60)
                                                                               GATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCC                                                                        >  SRR19973072.1680258/1‑150 (MQ=60)
                                                                                ATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCG                                                                       >  SRR19973072.3326165/1‑150 (MQ=60)
                                                                                ATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCG                                                                       >  SRR19973072.1888517/1‑150 (MQ=60)
                                                                                ATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCG                                                                       >  SRR19973072.4000261/1‑150 (MQ=60)
                                                                                 TGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGT                                                                      >  SRR19973072.4218802/1‑150 (MQ=60)
                                                                                 TGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGT                                                                      <  SRR19973072.1216605/150‑1 (MQ=60)
                                                                                  GCCCTGGACAGCGCGGCGTTGTTCTCGGGAGGCGTGGTACACTCTGTCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTG                                                                     <  SRR19973072.1380486/150‑1 (MQ=60)
                                                                                    CCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGAC                                                                   >  SRR19973072.1997143/1‑150 (MQ=60)
                                                                                     CTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACT                                                                  <  SRR19973072.1567677/150‑1 (MQ=60)
                                                                                         ACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTA                                                              >  SRR19973072.3627298/1‑150 (MQ=60)
                                                                                         ACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTA                                                              >  SRR19973072.3627583/1‑150 (MQ=60)
                                                                                              GCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCT                                                         <  SRR19973072.1530782/150‑1 (MQ=60)
                                                                                              GCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCT                                                         <  SRR19973072.2847870/150‑1 (MQ=60)
                                                                                              GCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCT                                                         <  SRR19973072.832436/150‑1 (MQ=60)
                                                                                                    TTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAG                                                   <  SRR19973072.740576/150‑1 (MQ=60)
                                                                                                     TGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGA                                                  >  SRR19973072.3585025/1‑150 (MQ=60)
                                                                                                     TGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGA                                                  >  SRR19973072.2922304/1‑150 (MQ=60)
                                                                                                     TGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGA                                                  >  SRR19973072.3034128/1‑150 (MQ=60)
                                                                                                        TCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTA                                               <  SRR19973072.4082520/150‑1 (MQ=60)
                                                                                                         CACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTAT                                              >  SRR19973072.2027742/1‑150 (MQ=60)
                                                                                                         CAAGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATAAGGCCGTGACGTTTATTGCTGTACAGAGTAT                                              >  SRR19973072.224555/1‑150 (MQ=60)
                                                                                                            GGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTG                                           >  SRR19973072.2922787/1‑150 (MQ=60)
                                                                                                            GGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTG                                           <  SRR19973072.3634565/150‑1 (MQ=60)
                                                                                                             GGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGG                                          <  SRR19973072.2902025/150‑1 (MQ=60)
                                                                                                             GGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGG                                          <  SRR19973072.2901835/150‑1 (MQ=60)
                                                                                                              GAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGT                                         >  SRR19973072.1671197/1‑150 (MQ=60)
                                                                                                                   GTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTC                                    <  SRR19973072.2856433/150‑1 (MQ=60)
                                                                                                                   GTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTC                                    <  SRR19973072.2856834/150‑1 (MQ=60)
                                                                                                                   GTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTC                                    <  SRR19973072.2856430/150‑1 (MQ=60)
                                                                                                                       TACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTG                                <  SRR19973072.746017/150‑1 (MQ=60)
                                                                                                                       TACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTG                                <  SRR19973072.4115039/150‑1 (MQ=60)
                                                                                                                        ACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGG                               <  SRR19973072.4369304/150‑1 (MQ=60)
                                                                                                                        ACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGG                               <  SRR19973072.4369318/150‑1 (MQ=60)
                                                                                                                          ACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCA                             <  SRR19973072.3940146/150‑1 (MQ=60)
                                                                                                                          ACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCA                             <  SRR19973072.3940089/150‑1 (MQ=60)
                                                                                                                            TCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATT                           <  SRR19973072.2218386/150‑1 (MQ=60)
                                                                                                                             CTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTA                          <  SRR19973072.3130107/150‑1 (MQ=60)
                                                                                                                              TGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAA                         <  SRR19973072.2598534/150‑1 (MQ=60)
                                                                                                                                GCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGG                       <  SRR19973072.3234153/150‑1 (MQ=60)
                                                                                                                                GCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGG                       >  SRR19973072.1238058/1‑150 (MQ=60)
                                                                                                                                GCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGG                       <  SRR19973072.2284877/150‑1 (MQ=60)
                                                                                                                                     CGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATA                  <  SRR19973072.880437/150‑1 (MQ=60)
                                                                                                                                     CGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATA                  <  SRR19973072.3208246/150‑1 (MQ=60)
                                                                                                                                     CGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATA                  <  SRR19973072.432487/150‑1 (MQ=60)
                                                                                                                                          GGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTC             <  SRR19973072.4564087/150‑1 (MQ=60)
                                                                                                                                                   AAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCT    <  SRR19973072.1170066/150‑1 (MQ=60)
                                                                                                                                                    AAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAAGTGATTGTTTGTTAGCAATATTAGGCCGTGACTTTTATTTCTGTAGAGATTATGTGGTTTTTCAGTGGCATTAAGGTCATATCTTCCCGCCGCCTC   >  SRR19973072.988317/1‑150 (MQ=60)
                                                                                                                                                     CACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCT  <  SRR19973072.655183/150‑1 (MQ=60)
                                                                                                                                                     AACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCT  <  SRR19973072.3316195/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
CGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCT  >  CP009273/4264915‑4265213

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑