Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,265,064 C→A 100% intergenic (‑95/‑335) yjcB ← / → yjcC putative inner membrane protein/putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,265,0640CA100.0% 746.9 / NA 227intergenic (‑95/‑335)yjcB/yjcCputative inner membrane protein/putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (96/131);  total (96/131)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 230 total aligned reads displayed.

ACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCG  >  CP009273/4264919‑4265208
                                                                                                                                                 |                                                                                                                                                
 cTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAaaca                                                                                                                                               >  1:518420/1‑148 (MQ=255)
  tAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACaa                                                                                                                                              <  1:2769247/148‑1 (MQ=255)
   aagaagTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACaaa                                                                                                                                             <  1:3426786/148‑1 (MQ=255)
    agaagTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACCATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACaaaa                                                                                                                                            <  2:1886257/148‑1 (MQ=255)
    agaagTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACaaaa                                                                                                                                            >  2:664282/1‑148 (MQ=255)
    agaagTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACaaaa                                                                                                                                            >  2:663841/1‑148 (MQ=255)
     gaagTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACaaaaa                                                                                                                                           >  2:2303155/1‑148 (MQ=255)
     gaagTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGGACACTCTGGCTATCGCGGGGCTTGCAGAAAACaaaaa                                                                                                                                           <  1:445380/148‑1 (MQ=255)
      aagTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAAt                                                                                                                                          >  2:862921/1‑148 (MQ=255)
         ttGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGaa                                                                                                                                       >  2:1489794/1‑148 (MQ=255)
            ccAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGCGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAaca                                                                                                                                    >  1:1566378/1‑148 (MQ=255)
            ccAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAaca                                                                                                                                    <  1:786141/148‑1 (MQ=255)
            ccAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAaca                                                                                                                                    <  1:3289854/148‑1 (MQ=255)
            ccAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAaca                                                                                                                                    <  2:3407122/148‑1 (MQ=255)
                cGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACActct                                                                                                                                >  2:412140/1‑148 (MQ=255)
                 gaagaagaaCCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCtg                                                                                                                               <  2:2772251/148‑1 (MQ=255)
                 gaagaagaaCCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCtg                                                                                                                               <  1:3270271/148‑1 (MQ=255)
                   agaagaaCCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCtgtt                                                                                                                             >  1:171261/1‑148 (MQ=255)
                    gaagaaCCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCtgttt                                                                                                                            >  1:1846507/1‑148 (MQ=255)
                     aagaaCCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCtgtttg                                                                                                                           <  1:2793509/148‑1 (MQ=255)
                        aaCCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCtgtttgtat                                                                                                                        >  1:786917/1‑146 (MQ=255)
                        aaCCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCtgtttgtat                                                                                                                        >  2:2625009/1‑146 (MQ=255)
                          ccACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCa                                                                                                                      >  2:2135379/1‑148 (MQ=255)
                          ccACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCa                                                                                                                      <  1:1280796/148‑1 (MQ=255)
                          ccACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCa                                                                                                                      >  2:1847819/1‑148 (MQ=255)
                          ccACGCCAGTGGTGAGGGTAGCCATCAAACAAGTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCa                                                                                                                      <  2:1178683/148‑1 (MQ=255)
                             cGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTa                                                                                                                   <  1:1184345/148‑1 (MQ=255)
                             cGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTa                                                                                                                   <  1:2934602/148‑1 (MQ=255)
                             cGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTa                                                                                                                   >  2:1989461/1‑148 (MQ=255)
                               ccAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAAt                                                                                                                 <  1:1796542/148‑1 (MQ=255)
                                 aGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAAttt                                                                                                               >  1:1621901/1‑148 (MQ=255)
                                    ggtgAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTtgt                                                                                                            <  1:2534318/148‑1 (MQ=255)
                                     gtgAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTtgtg                                                                                                           >  2:1207601/1‑148 (MQ=255)
                                       gAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGaa                                                                                                         <  2:2071624/148‑1 (MQ=255)
                                       gAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGaa                                                                                                         <  2:301247/148‑1 (MQ=255)
                                         gggTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACt                                                                                                       <  1:38/148‑1 (MQ=255)
                                           gTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACtat                                                                                                     >  1:1933417/1‑148 (MQ=255)
                                             aGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTatat                                                                                                   <  2:1599085/148‑1 (MQ=255)
                                              gCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATc                                                                                                  >  1:1060041/1‑148 (MQ=255)
                                                 aTCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATcaca                                                                                               <  2:3268541/148‑1 (MQ=255)
                                                 aTCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATcaca                                                                                               <  1:1677004/148‑1 (MQ=255)
                                                    aaaCAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAatt                                                                                            >  1:3382568/1‑148 (MQ=255)
                                                     aaCAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAattg                                                                                           >  1:2557247/1‑148 (MQ=255)
                                                       cAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAattgat                                                                                         >  2:1536480/1‑148 (MQ=255)
                                                       cAAGTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAattgat                                                                                         <  2:1341255/148‑1 (MQ=255)
                                                        aaTTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAattgatt                                                                                        <  1:1490077/148‑1 (MQ=255)
                                                        aaTTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAattgatt                                                                                        <  2:2722962/148‑1 (MQ=255)
                                                         aTTCCTCTGAAAAGAGCGGATGCCCGGGACAGCGCGGCGTTGGTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAtttgattg                                                                                       <  2:1004401/148‑1 (MQ=255)
                                                            ccTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGAttgttt                                                                                    >  2:228828/1‑148 (MQ=255)
                                                              tctGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGAttgtttgt                                                                                  <  2:2676357/148‑1 (MQ=255)
                                                              tctGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGAttgtttgt                                                                                  <  2:1638249/148‑1 (MQ=255)
                                                               ctGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATtgtttgtt                                                                                 <  2:459476/148‑1 (MQ=255)
                                                                 gAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAg                                                                               >  2:587450/1‑148 (MQ=255)
                                                                  aaaaGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGc                                                                              <  1:2287345/148‑1 (MQ=255)
                                                                  aaaaGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGc                                                                              <  2:705336/148‑1 (MQ=255)
                                                                  aaaaGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGc                                                                              >  1:172935/1‑148 (MQ=255)
                                                                    aaGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCa                                                                            <  1:3062036/148‑1 (MQ=255)
                                                                    aaGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCa                                                                            <  1:2085821/148‑1 (MQ=255)
                                                                      gagCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCata                                                                          <  1:1247936/148‑1 (MQ=255)
                                                                      gagCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCata                                                                          >  1:2576190/1‑148 (MQ=255)
                                                                        gCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATAtt                                                                        <  1:3180768/148‑1 (MQ=255)
                                                                             tGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGcc                                                                   <  1:2787800/148‑1 (MQ=255)
                                                                                    gACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACtt                                                            >  2:1144421/1‑148 (MQ=255)
                                                                                     aCAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACttt                                                           <  2:3029761/148‑1 (MQ=255)
                                                                                      cAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACtttt                                                          >  2:3107103/1‑148 (MQ=255)
                                                                                      cAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACGATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACtttt                                                          <  2:1203316/148‑1 (MQ=255)
                                                                                          gcggcgTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGAATTTTATTg                                                      <  1:2514053/148‑1 (MQ=255)
                                                                                           cggcgTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGc                                                     <  2:2770252/148‑1 (MQ=255)
                                                                                            ggcgTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCt                                                    <  1:1485285/148‑1 (MQ=255)
                                                                                             gcgTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTg                                                   <  1:2625009/148‑1 (MQ=255)
                                                                                                 tgttCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACa                                               >  1:3088809/1‑148 (MQ=255)
                                                                                                  gttCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAg                                              <  1:1759148/148‑1 (MQ=255)
                                                                                                    tCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGAt                                            >  2:2632420/1‑148 (MQ=255)
                                                                                                    tCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGAt                                            <  2:3241104/148‑1 (MQ=255)
                                                                                                       cGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTAt                                         <  1:203846/148‑1 (MQ=255)
                                                                                                         ggAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTAtgt                                       <  2:2249283/148‑1 (MQ=255)
                                                                                                         ggAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTAtgt                                       >  2:2548904/1‑148 (MQ=255)
                                                                                                          gAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTAtgtg                                      <  2:1727864/148‑1 (MQ=255)
                                                                                                          gAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTAtgtg                                      <  2:3010047/148‑1 (MQ=255)
                                                                                                          gAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTAtgtg                                      >  1:298280/1‑148 (MQ=255)
                                                                                                           aGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTgg                                     <  2:416581/148‑1 (MQ=255)
                                                                                                             gCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGtt                                   <  1:2031942/148‑1 (MQ=255)
                                                                                                             gCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGtt                                   <  1:1668200/148‑1 (MQ=255)
                                                                                                                 ggTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTc                               <  1:1129859/148‑1 (MQ=255)
                                                                                                                 ggTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTc                               <  1:1331979/148‑1 (MQ=255)
                                                                                                                      acTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTgg                          >  2:753182/1‑148 (MQ=255)
                                                                                                                          tGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCCTATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCAtt                      >  1:2386408/1‑148 (MQ=255)
                                                                                                                           ggCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTa                     >  1:1618793/1‑148 (MQ=255)
                                                                                                                             cTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAg                   <  2:2903542/148‑1 (MQ=255)
                                                                                                                                tcacgGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGc                <  2:1469710/145‑1 (MQ=255)
                                                                                                                                  gcgGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCat              >  1:2037712/1‑148 (MQ=255)
                                                                                                                                  gcgGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCat              <  2:1924568/148‑1 (MQ=255)
                                                                                                                                   cgGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCata             <  2:1133471/148‑1 (MQ=255)
                                                                                                                                    ggggCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCatat            <  1:830845/148‑1 (MQ=255)
                                                                                                                                       gCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCtt         <  2:3300181/148‑1 (MQ=255)
                                                                                                                                       gCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCtt         >  2:33205/1‑148 (MQ=255)
                                                                                                                                       gCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCtt         >  2:1654587/1‑148 (MQ=255)
                                                                                                                                        cTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTc        <  1:2302819/148‑1 (MQ=255)
                                                                                                                                        cTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTc        <  2:508285/148‑1 (MQ=255)
                                                                                                                                           gCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCccg     <  1:1198917/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
ACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCG  >  CP009273/4264919‑4265208

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCT  >  CP009273/4264915‑4265213
                                                                                                                                                     |                                                                                                                                                     
CGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAA                                                                                                                                                       >  SRR19973065.3104567/1‑150 (MQ=60)
  GCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAAC                                                                                                                                                     <  SRR19973065.591930/150‑1 (MQ=60)
  CCACTAATAAGTTGCCTGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAAGTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAAC                                                                                                                                                     <  SRR19973065.773140/150‑1 (MQ=60)
   CACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACA                                                                                                                                                    <  SRR19973065.2135053/150‑1 (MQ=60)
   CACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACA                                                                                                                                                    <  SRR19973065.2135039/150‑1 (MQ=60)
   CACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACA                                                                                                                                                    >  SRR19973065.521843/1‑150 (MQ=60)
      TAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAA                                                                                                                                                 <  SRR19973065.2786450/150‑1 (MQ=60)
       AAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAA                                                                                                                                                <  SRR19973065.3447698/150‑1 (MQ=60)
       AAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAA                                                                                                                                                >  SRR19973065.2569161/1‑150 (MQ=60)
        AGAAGTTGCCAGCGAAGATGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAAT                                                                                                                                               <  SRR19973065.2578939/150‑1 (MQ=60)
        AGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAAT                                                                                                                                               <  SRR19973065.2578412/150‑1 (MQ=60)
         GAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGGACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATG                                                                                                                                              <  SRR19973065.448464/150‑1 (MQ=60)
              TGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGCGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACA                                                                                                                                         >  SRR19973065.1576791/1‑150 (MQ=60)
                CCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACT                                                                                                                                       <  SRR19973065.3309614/150‑1 (MQ=60)
                CCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACT                                                                                                                                       <  SRR19973065.791366/150‑1 (MQ=60)
                CCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACT                                                                                                                                       <  SRR19973065.791352/150‑1 (MQ=60)
                CCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACT                                                                                                                                       <  SRR19973065.3309873/150‑1 (MQ=60)
                  AGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCT                                                                                                                                     <  SRR19973065.1788281/150‑1 (MQ=60)
                   GCGAATAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTG                                                                                                                                    <  SRR19973065.3028048/150‑1 (MQ=60)
                   GCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTG                                                                                                                                    <  SRR19973065.1248438/150‑1 (MQ=60)
                   GCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTG                                                                                                                                    <  SRR19973065.1269112/150‑1 (MQ=60)
                   GCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTG                                                                                                                                    <  SRR19973065.3028194/150‑1 (MQ=60)
                     GAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTT                                                                                                                                  >  SRR19973065.172632/1‑150 (MQ=60)
                     GAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTT                                                                                                                                  >  SRR19973065.2315663/1‑150 (MQ=60)
                     GAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTT                                                                                                                                  <  SRR19973065.3290112/150‑1 (MQ=60)
                      AAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTT                                                                                                                                 >  SRR19973065.1858674/1‑150 (MQ=60)
                         AAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTA                                                                                                                              <  SRR19973065.2810821/150‑1 (MQ=60)
                          AGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTAT                                                                                                                             >  SRR19973065.792133/1‑150 (MQ=60)
                          AGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTAT                                                                                                                             >  SRR19973065.1739319/1‑150 (MQ=60)
                           GAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTC                                                                                                                            >  SRR19973065.1572489/1‑150 (MQ=60)
                              CCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATT                                                                                                                         <  SRR19973065.1289252/150‑1 (MQ=60)
                                 CGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAAT                                                                                                                      >  SRR19973065.63346/1‑150 (MQ=60)
                                 CGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAAT                                                                                                                      <  SRR19973065.2952401/150‑1 (MQ=60)
                                 CGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAAT                                                                                                                      <  SRR19973065.1191844/150‑1 (MQ=60)
                                   CCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTT                                                                                                                    >  SRR19973065.695522/1‑150 (MQ=60)
                                   CCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTT                                                                                                                    >  SRR19973065.1632773/1‑150 (MQ=60)
                                   CCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTT                                                                                                                    <  SRR19973065.1807232/150‑1 (MQ=60)
                                   CCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTT                                                                                                                    <  SRR19973065.1808341/150‑1 (MQ=60)
                                   CCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTT                                                                                                                    >  SRR19973065.1632254/1‑150 (MQ=60)
                                        GGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGA                                                                                                               <  SRR19973065.2550220/150‑1 (MQ=60)
                                        GGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGA                                                                                                               <  SRR19973065.2546476/150‑1 (MQ=60)
                                        GGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGA                                                                                                               <  SRR19973065.1951353/150‑1 (MQ=60)
                                          TGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAAC                                                                                                             <  SRR19973065.3284059/150‑1 (MQ=60)
                                            AGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTA                                                                                                           <  SRR19973065.1670053/150‑1 (MQ=60)
                                             GGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTAT                                                                                                          <  SRR19973065.39/150‑1 (MQ=60)
                                             GGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTAT                                                                                                          >  SRR19973065.1945994/1‑150 (MQ=60)
                                             GGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTAT                                                                                                          >  SRR19973065.1946073/1‑150 (MQ=60)
                                                TAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATC                                                                                                       >  SRR19973065.1067068/1‑150 (MQ=60)
                                                TAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATC                                                                                                       >  SRR19973065.2829467/1‑150 (MQ=60)
                                                TAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATC                                                                                                       <  SRR19973065.897366/150‑1 (MQ=60)
                                                    CATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAA                                                                                                   <  SRR19973065.2052801/150‑1 (MQ=60)
                                                    CATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACACTGTTTGTATCATTAATTTTGTGAACTATATCACAA                                                                                                   >  SRR19973065.181134/1‑150 (MQ=60)
                                                     ATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAAT                                                                                                  <  SRR19973065.1688976/150‑1 (MQ=60)
                                                     ATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAAT                                                                                                  <  SRR19973065.1688220/150‑1 (MQ=60)
                                                      TCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATT                                                                                                 >  SRR19973065.3403250/1‑150 (MQ=60)
                                                      TCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATT                                                                                                 <  SRR19973065.2152731/150‑1 (MQ=60)
                                                       CAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTG                                                                                                >  SRR19973065.2573239/1‑150 (MQ=60)
                                                         AACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGAT                                                                                              >  SRR19973065.252135/1‑150 (MQ=60)
                                                         AACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGAT                                                                                              >  SRR19973065.252214/1‑150 (MQ=60)
                                                          ACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATT                                                                                             >  SRR19973065.3368718/1‑150 (MQ=60)
                                                            AATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGT                                                                                           <  SRR19973065.1499340/150‑1 (MQ=60)
                                                            AATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGT                                                                                           <  SRR19973065.1499629/150‑1 (MQ=60)
                                                             ATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTT                                                                                          >  SRR19973065.1711214/1‑150 (MQ=60)
                                                             ATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTT                                                                                          <  SRR19973065.2168745/150‑1 (MQ=60)
                                                                CCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCACGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGT                                                                                       >  SRR19973065.1479132/1‑150 (MQ=60)
                                                                    TGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGC                                                                                   >  SRR19973065.2790154/1‑150 (MQ=60)
                                                                    TGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGC                                                                                   >  SRR19973065.174317/1‑150 (MQ=60)
                                                                      AAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCA                                                                                 <  SRR19973065.2301759/150‑1 (MQ=60)
                                                                        AAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATA                                                                               <  SRR19973065.2099192/150‑1 (MQ=60)
                                                                        AAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATA                                                                               >  SRR19973065.2592258/1‑150 (MQ=60)
                                                                        AAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATA                                                                               <  SRR19973065.3080756/150‑1 (MQ=60)
                                                                          GAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATT                                                                             <  SRR19973065.356506/150‑1 (MQ=60)
                                                                          GAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATT                                                                             <  SRR19973065.1256155/150‑1 (MQ=60)
                                                                          GAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATT                                                                             <  SRR19973065.356938/150‑1 (MQ=60)
                                                                            gcggtTGCCCTGGACTGGGCGGAGTTGTTCTCGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAATATGAAACACTCTGTTTGTATCATTTATTTTGTGAACTATCTCACAATTGATTGTTTGTTAGCCATATTAG                                                                           <  SRR19973065.3199806/145‑1 (MQ=60)
                                                                            GCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAG                                                                           <  SRR19973065.3199988/150‑1 (MQ=60)
                                                                             CCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGG                                                                          <  SRR19973065.1162491/150‑1 (MQ=60)
                                                                                 TGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGT                                                                      <  SRR19973065.2805085/150‑1 (MQ=60)
                                                                                       GGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTTAACTATATCACAATTGATTGTTTGTTATCCATATTAGTCCGTTACTTT                                                                >  SRR19973065.3029275/1‑150 (MQ=60)
                                                                                           AGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATT                                                            <  SRR19973065.3221647/150‑1 (MQ=60)
                                                                                              GCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGAATTTTATTGCT                                                         <  SRR19973065.2529849/150‑1 (MQ=60)
                                                                                                GGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGT                                                       <  SRR19973065.1494759/150‑1 (MQ=60)
                                                                                                GGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGT                                                       <  SRR19973065.1494740/150‑1 (MQ=60)
                                                                                                 GCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTA                                                      <  SRR19973065.2641242/150‑1 (MQ=60)
                                                                                                 GCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTA                                                      <  SRR19973065.2641250/150‑1 (MQ=60)
                                                                                                   GTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACA                                                    >  SRR19973065.3107670/1‑150 (MQ=60)
                                                                                                      GTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGAT                                                 <  SRR19973065.1770704/150‑1 (MQ=60)
                                                                                                      GTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGAT                                                 <  SRR19973065.1770321/150‑1 (MQ=60)
                                                                                                           CGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGT                                            <  SRR19973065.205409/150‑1 (MQ=60)
                                                                                                            GGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTG                                           >  SRR19973065.300207/1‑150 (MQ=60)
                                                                                                            GGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTG                                           >  SRR19973065.299349/1‑150 (MQ=60)
                                                                                                              GAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTCGGCCGTGACTTTTATTGCTGTACAGATTATGTGGT                                         >  SRR19973065.436005/1‑150 (MQ=60)
                                                                                                                 GCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTT                                      <  SRR19973065.1679363/150‑1 (MQ=60)
                                                                                                                 GCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTT                                      <  SRR19973065.2044983/150‑1 (MQ=60)
                                                                                                                 GCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTT                                      <  SRR19973065.2045016/150‑1 (MQ=60)
                                                                                                                  CGTGGTACACTCTGGCTATCGCGGGGGTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTT                                     <  SRR19973065.1096197/150‑1 (MQ=60)
                                                                                                                     GGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAG                                  <  SRR19973065.3098706/150‑1 (MQ=60)
                                                                                                                     GGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAG                                  >  SRR19973065.1612912/1‑150 (MQ=60)
                                                                                                                     GGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAG                                  <  SRR19973065.1340790/150‑1 (MQ=60)
                                                                                                                     GGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAG                                  <  SRR19973065.2808985/150‑1 (MQ=60)
                                                                                                                     GGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAG                                  <  SRR19973065.2808880/150‑1 (MQ=60)
                                                                                                                     GGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAG                                  <  SRR19973065.1137201/150‑1 (MQ=60)
                                                                                                                       TACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTG                                >  SRR19973065.1684964/1‑150 (MQ=60)
                                                                                                                          ACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCA                             >  SRR19973065.1319254/1‑150 (MQ=60)
                                                                                                                          ACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCA                             <  SRR19973065.2718406/150‑1 (MQ=60)
                                                                                                                            TCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCCTATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATT                           >  SRR19973065.2401377/1‑150 (MQ=60)
                                                                                                                            TCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATT                           <  SRR19973065.952714/150‑1 (MQ=60)
                                                                                                                             CTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTA                          >  SRR19973065.1629281/1‑150 (MQ=60)
                                                                                                                             CTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTA                          >  SRR19973065.1629651/1‑150 (MQ=60)
                                                                                                                                 CTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGG                      <  SRR19973065.1217211/150‑1 (MQ=60)
                                                                                                                                    TCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCAT                   <  SRR19973065.1198736/150‑1 (MQ=60)
                                                                                                                                    TCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCAT                   >  SRR19973065.2050769/1‑150 (MQ=60)
                                                                                                                                    TCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCAGATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCAT                   >  SRR19973065.2050848/1‑150 (MQ=60)
                                                                                                                                      GCGGGGCTTGCAGAAAACAAAAATTAAACACTCTGTTTTTATAATTAATTGTGTGAACTATATCTCAATTTATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATAT                 <  SRR19973065.1986427/150‑1 (MQ=60)
                                                                                                                                      GCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATAT                 <  SRR19973065.3432730/150‑1 (MQ=60)
                                                                                                                                        GGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCT               >  SRR19973065.802906/1‑150 (MQ=60)
                                                                                                                                        GGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCT               <  SRR19973065.836272/150‑1 (MQ=60)
                                                                                                                                            CTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCC           <  SRR19973065.2317303/150‑1 (MQ=60)
                                                                                                                                            CTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCC           <  SRR19973065.2317639/150‑1 (MQ=60)
                                                                                                                                              TGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGC         >  SRR19973065.491809/1‑150 (MQ=60)
                                                                                                                                               GCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCC        <  SRR19973065.1206575/150‑1 (MQ=60)
                                                                                                                                                   AAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGAGTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCT    >  SRR19973065.127711/1‑150 (MQ=60)
                                                                                                                                                    AAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTC   >  SRR19973065.290161/1‑150 (MQ=60)
                                                                                                                                                     AACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCT  <  SRR19973065.3026357/150‑1 (MQ=60)
                                                                                                                                                     AACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCT  <  SRR19973065.3413034/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
CGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCT  >  CP009273/4264915‑4265213

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑