Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,265,064 C→A 100% intergenic (‑95/‑335) yjcB ← / → yjcC putative inner membrane protein/putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,265,0640CA99.6% 804.7 / ‑4.3 241intergenic (‑95/‑335)yjcB/yjcCputative inner membrane protein/putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base A (115/125);  total (115/126)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 242 total aligned reads displayed.

CTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCG  >  CP009273/4264920‑4265208
                                                                                                                                                |                                                                                                                                                
cTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAaaca                                                                                                                                               >  1:18461/1‑148 (MQ=255)
 tAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACaa                                                                                                                                              <  2:3330373/148‑1 (MQ=255)
   agaagTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACaaaa                                                                                                                                            <  2:1433203/148‑1 (MQ=255)
      agTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATg                                                                                                                                         >  2:849580/1‑148 (MQ=255)
      agTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATg                                                                                                                                         <  2:270/148‑1 (MQ=255)
      agTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATg                                                                                                                                         >  1:1778642/1‑148 (MQ=255)
        ttGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCACACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGaa                                                                                                                                       <  2:2879466/148‑1 (MQ=255)
        ttGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGaa                                                                                                                                       <  1:1147057/148‑1 (MQ=255)
          gCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAac                                                                                                                                     <  2:614836/148‑1 (MQ=255)
          gCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAac                                                                                                                                     <  1:2943275/148‑1 (MQ=255)
            cAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAacac                                                                                                                                   <  1:499682/148‑1 (MQ=255)
              gCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACActc                                                                                                                                 >  2:730462/1‑148 (MQ=255)
              gCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACActc                                                                                                                                 <  2:1664542/148‑1 (MQ=255)
               cGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACActct                                                                                                                                >  1:11735/1‑148 (MQ=255)
               cGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACActct                                                                                                                                <  2:1786039/148‑1 (MQ=255)
                gaagaagaaCCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGGGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCtg                                                                                                                               <  1:2562240/148‑1 (MQ=255)
                  agaagaaCCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCtgtt                                                                                                                             >  1:2352451/1‑148 (MQ=255)
                  agaagaaCCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCtgtt                                                                                                                             <  1:1282769/148‑1 (MQ=255)
                  agaagaaCCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCtgtt                                                                                                                             <  2:3227392/148‑1 (MQ=255)
                      gaaCCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCtgtttgta                                                                                                                         <  2:2424608/148‑2 (MQ=255)
                       aaCCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCtgtttgtat                                                                                                                        <  1:2822993/148‑3 (MQ=255)
                                 gtggtgAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAAtttt                                                                                                              >  1:2923583/1‑148 (MQ=255)
                                 gtggtgAGGGTAGCCATCAAACAAGTCCTCTGAAAAGAGCCGAGGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAAtttt                                                                                                              <  2:1595606/148‑1 (MQ=255)
                                  tggtgAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTtg                                                                                                             >  1:2960817/1‑148 (MQ=255)
                                      gAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGaa                                                                                                         <  2:2564576/148‑1 (MQ=255)
                                        gggTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACt                                                                                                       <  2:140564/148‑1 (MQ=255)
                                        gggTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACt                                                                                                       <  2:2914749/148‑1 (MQ=255)
                                           tAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACtata                                                                                                    <  2:2896334/148‑1 (MQ=255)
                                           tAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACtata                                                                                                    >  1:807548/1‑148 (MQ=255)
                                           tAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACtata                                                                                                    <  2:3241065/148‑1 (MQ=255)
                                                aTCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATcaca                                                                                               <  1:567960/148‑1 (MQ=255)
                                                 tCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACaa                                                                                              >  1:2113534/1‑148 (MQ=255)
                                                 tCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACaa                                                                                              <  1:857075/148‑1 (MQ=255)
                                                 tCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACaa                                                                                              <  1:2638259/148‑1 (MQ=255)
                                                  cAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAat                                                                                             <  1:636535/148‑1 (MQ=255)
                                                   aaaCAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAatt                                                                                            >  2:1113901/1‑148 (MQ=255)
                                                    aaCAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAattg                                                                                           <  2:1858854/148‑1 (MQ=255)
                                                     aCAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAattaa                                                                                          >  1:3531338/1‑146 (MQ=255)
                                                       aaTTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAattgatt                                                                                        >  2:1216642/1‑148 (MQ=255)
                                                        aTTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAattgattg                                                                                       >  2:2252109/1‑148 (MQ=255)
                                                        aTTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAattgattg                                                                                       >  2:1063670/1‑148 (MQ=255)
                                                        aTTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAattgattg                                                                                       >  2:47415/1‑148 (MQ=255)
                                                        aTTCCTCTGAAAAGAGCCGAAGCCCTGGACAGCGCGTCGTTTTTAACGGGAGGCGTGTTAAACTCTGCCTATCGCGGGGCTTGCAGAAAACAAAAATGAAAAACTCTGTTTGTATAATTAATTTTTTCAACTATATCACAatttattg                                                                                       >  2:773418/1‑148 (MQ=255)
                                                         ttCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGAttgt                                                                                      <  1:2734112/148‑1 (MQ=255)
                                                             tctGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGAttgtttgt                                                                                  >  1:566598/1‑148 (MQ=255)
                                                              ctGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATtgtttgtt                                                                                 <  1:1956966/148‑1 (MQ=255)
                                                              ctGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATtgtttgtt                                                                                 <  1:1208674/148‑1 (MQ=255)
                                                                gAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAg                                                                               <  1:1400114/148‑1 (MQ=255)
                                                                 aaaaGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGc                                                                              >  2:553023/1‑148 (MQ=255)
                                                                 aaaaGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGc                                                                              <  1:2713865/148‑1 (MQ=255)
                                                                   aaGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCa                                                                            >  1:8724/1‑148 (MQ=255)
                                                                   aaGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCa                                                                            >  1:1609361/1‑148 (MQ=255)
                                                                   aaGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCa                                                                            >  2:669753/1‑148 (MQ=255)
                                                                    agagCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCat                                                                           <  2:1562237/148‑1 (MQ=255)
                                                                    agagCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCat                                                                           >  1:2862311/1‑148 (MQ=255)
                                                                     gagCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCata                                                                          >  1:1719597/1‑148 (MQ=255)
                                                                      agCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCatat                                                                         >  1:2301653/1‑148 (MQ=255)
                                                                      agCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCatat                                                                         >  1:2121076/1‑148 (MQ=255)
                                                                      agCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCatat                                                                         >  1:908012/1‑148 (MQ=255)
                                                                       gCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATAtt                                                                        <  1:2417079/148‑1 (MQ=255)
                                                                        ccGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGTGCTTGCAGAAAACAAATATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACTATTGATTGTTTGTTATCCATATTa                                                                       <  1:3509150/148‑1 (MQ=255)
                                                                        ccGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTa                                                                       <  2:2034510/148‑1 (MQ=255)
                                                                        ccGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAAGTGATTGTTTGTTAGCCATATTa                                                                       >  2:3366701/1‑148 (MQ=255)
                                                                          gATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAgg                                                                     <  1:293454/148‑1 (MQ=255)
                                                                          gATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAgg                                                                     >  1:2260786/1‑148 (MQ=255)
                                                                           aTGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGc                                                                    >  1:1387477/1‑148 (MQ=255)
                                                                                cTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGa                                                               <  1:3441184/148‑1 (MQ=255)
                                                                                  ggACAGCGCGGCGTTGTTCACGGGATGCGTGGTACACTCTTGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTTAAATATATCACAAGTGATTGTTTGTTAGCCATATTAGGCCGTGACt                                                             >  1:2922115/1‑148 (MQ=255)
                                                                                  ggACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACt                                                             <  2:1979895/148‑1 (MQ=255)
                                                                                   gACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAAttt                                                                                                               >  1:2549558/1‑97 (MQ=255)
                                                                                   gACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAAttt                                                                                                               <  2:2549558/97‑1 (MQ=255)
                                                                                   gACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTATGCCGTGACtt                                                            >  1:938306/1‑148 (MQ=255)
                                                                                    aCAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACttt                                                           <  2:2193480/148‑1 (MQ=255)
                                                                                    aCAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACttt                                                           >  1:1977349/1‑148 (MQ=255)
                                                                                       gcgaGGCGTTGTTCACGGGATGCGTGGTTCACTCTTGAGATCTCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTAt                                                        <  2:3293563/148‑1 (MQ=255)
                                                                                          cggcgTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGc                                                     >  2:1236061/1‑148 (MQ=255)
                                                                                               ttgttCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTAc                                                <  1:3309844/148‑1 (MQ=255)
                                                                                                tgttCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACa                                               >  2:2712435/1‑148 (MQ=255)
                                                                                                   tCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGAt                                            >  2:3089777/1‑148 (MQ=255)
                                                                                                     aCGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTa                                          <  1:730463/148‑1 (MQ=255)
                                                                                                       gggAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTAtg                                        <  2:2352451/148‑1 (MQ=255)
                                                                                                          atgCGTGGTACACTCTGGCTATCGAGGGTCTTTCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTgg                                     <  1:3115659/146‑1 (MQ=255)
                                                                                                          aGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTgg                                     <  1:774382/148‑1 (MQ=255)
                                                                                                            gCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGtt                                   <  2:2113534/148‑1 (MQ=255)
                                                                                                                ggTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTc                               <  2:3074764/148‑1 (MQ=255)
                                                                                                                  tACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAg                             <  1:222632/148‑1 (MQ=255)
                                                                                                                  tACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAg                             <  1:2019307/148‑1 (MQ=255)
                                                                                                                  tACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAg                             <  1:1423712/148‑1 (MQ=255)
                                                                                                                        ctGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCAt                       >  1:3428820/1‑148 (MQ=255)
                                                                                                                        ctGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCAt                       <  2:234111/148‑1 (MQ=255)
                                                                                                                         tGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCAtt                      <  2:11735/148‑1 (MQ=255)
                                                                                                                          ggCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTa                     <  2:3499837/148‑1 (MQ=255)
                                                                                                                           gCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTaa                    >  2:3349791/1‑148 (MQ=255)
                                                                                                                           gCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTaa                    >  2:704065/1‑148 (MQ=255)
                                                                                                                             tATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAgg                  <  2:507573/148‑1 (MQ=255)
                                                                                                                              aTCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAggg                 >  1:1302880/1‑148 (MQ=255)
                                                                                                                               tCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGc                >  1:382239/1‑148 (MQ=255)
                                                                                                                                       cTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTc        <  2:3461486/148‑1 (MQ=255)
                                                                                                                                        ttGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTcc       <  1:310659/148‑1 (MQ=255)
                                                                                                                                            aGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACGTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCccgcc   >  2:2512300/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                
CTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCG  >  CP009273/4264920‑4265208

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCT  >  CP009273/4264916‑4265213
                                                                                                                                                    |                                                                                                                                                     
GGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAA                                                                                                                                                      <  SRR19973067.747514/150‑1 (MQ=60)
 GCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAAC                                                                                                                                                     <  SRR19973067.763181/150‑1 (MQ=60)
  CACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACA                                                                                                                                                    >  SRR19973067.18739/1‑150 (MQ=60)
  CACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACA                                                                                                                                                    <  SRR19973067.1588460/150‑1 (MQ=60)
   ACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAA                                                                                                                                                   >  SRR19973067.871013/1‑150 (MQ=60)
   ACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAA                                                                                                                                                   >  SRR19973067.871012/1‑150 (MQ=60)
    CTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAA                                                                                                                                                  >  SRR19973067.141807/1‑150 (MQ=60)
     TAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAA                                                                                                                                                 <  SRR19973067.571456/150‑1 (MQ=60)
      AAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAA                                                                                                                                                >  SRR19973067.512017/1‑150 (MQ=60)
      AAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAA                                                                                                                                                >  SRR19973067.511040/1‑150 (MQ=60)
        GAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATG                                                                                                                                              >  SRR19973067.281236/1‑150 (MQ=60)
        GAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATG                                                                                                                                              >  SRR19973067.1790680/1‑150 (MQ=60)
         AAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGA                                                                                                                                             <  SRR19973067.2076335/150‑1 (MQ=60)
          AGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAA                                                                                                                                            >  SRR19973067.1176855/1‑150 (MQ=60)
           GTTGCCAGCGAAGAAGAACCACTCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTTGACAGCGCGGAGTTGTTCACGGGACGCCTGGTACACACTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAA                                                                                                                                           >  SRR19973067.2422528/1‑150 (MQ=60)
            TTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAAC                                                                                                                                          <  SRR19973067.81077/150‑1 (MQ=60)
            TTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAAC                                                                                                                                          <  SRR19973067.81082/150‑1 (MQ=60)
            TTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAAC                                                                                                                                          <  SRR19973067.1154745/150‑1 (MQ=60)
             TGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACA                                                                                                                                         <  SRR19973067.2151538/150‑1 (MQ=60)
              GCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACAC                                                                                                                                        <  SRR19973067.2961923/150‑1 (MQ=60)
              GCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACAC                                                                                                                                        <  SRR19973067.2961928/150‑1 (MQ=60)
                CAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTC                                                                                                                                      <  SRR19973067.503115/150‑1 (MQ=60)
                CAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTC                                                                                                                                      <  SRR19973067.2517555/150‑1 (MQ=60)
                CAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTC                                                                                                                                      <  SRR19973067.769124/150‑1 (MQ=60)
                 AGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCT                                                                                                                                     <  SRR19973067.2244679/150‑1 (MQ=60)
                 AGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAAAGAAACACTCT                                                                                                                                     >  SRR19973067.11920/1‑150 (MQ=60)
                  GCTAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTG                                                                                                                                    <  SRR19973067.2825557/150‑1 (MQ=60)
                    GAAGATGAACCACGCGAGTGGTGTGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGGGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTT                                                                                                                                  <  SRR19973067.2578982/150‑1 (MQ=60)
                    GAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTT                                                                                                                                  >  SRR19973067.1236670/1‑150 (MQ=60)
                    GAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTT                                                                                                                                  >  SRR19973067.1236742/1‑150 (MQ=60)
                    GAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTT                                                                                                                                  >  SRR19973067.362834/1‑150 (MQ=60)
                    GAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGGT                                                                                                                                  >  SRR19973067.2367805/1‑150 (MQ=60)
                      AGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTG                                                                                                                                >  SRR19973067.1342475/1‑150 (MQ=60)
                      AGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTG                                                                                                                                <  SRR19973067.1291511/150‑1 (MQ=60)
                          GAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATC                                                                                                                            >  SRR19973067.2707662/1‑150 (MQ=60)
                           AACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCA                                                                                                                           <  SRR19973067.2841156/150‑1 (MQ=60)
                                CGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAAT                                                                                                                      <  SRR19973067.1678754/150‑1 (MQ=60)
                                 GCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATT                                                                                                                     <  SRR19973067.1974258/150‑1 (MQ=60)
                                   CAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTT                                                                                                                   >  SRR19973067.2942163/1‑150 (MQ=60)
                                   CAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTT                                                                                                                   >  SRR19973067.2942166/1‑150 (MQ=60)
                                    AGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTG                                                                                                                  <  SRR19973067.3503419/150‑1 (MQ=60)
                                    AGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTG                                                                                                                  >  SRR19973067.2979519/1‑150 (MQ=60)
                                      TGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTG                                                                                                                <  SRR19973067.2128349/150‑1 (MQ=60)
                                      TGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTG                                                                                                                <  SRR19973067.2318614/150‑1 (MQ=60)
                                           AGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTA                                                                                                           >  SRR19973067.3441333/1‑150 (MQ=60)
                                             GGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATA                                                                                                         >  SRR19973067.813099/1‑150 (MQ=60)
                                             GGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATA                                                                                                         <  SRR19973067.2859663/150‑1 (MQ=60)
                                                AGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCA                                                                                                      >  SRR19973067.3198923/1‑150 (MQ=60)
                                                AGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCA                                                                                                      >  SRR19973067.2568489/1‑150 (MQ=60)
                                                 GCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCAC                                                                                                     <  SRR19973067.365493/150‑1 (MQ=60)
                                                   CATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAA                                                                                                   >  SRR19973067.2127608/1‑150 (MQ=60)
                                                    ATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAAT                                                                                                  <  SRR19973067.1197617/150‑1 (MQ=60)
                                                    ATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAAT                                                                                                  <  SRR19973067.571654/150‑1 (MQ=60)
                                                    ATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAAT                                                                                                  >  SRR19973067.2896464/1‑150 (MQ=60)
                                                    ATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAAT                                                                                                  >  SRR19973067.2894640/1‑150 (MQ=60)
                                                     TCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATT                                                                                                 <  SRR19973067.1933767/150‑1 (MQ=60)
                                                     TCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATT                                                                                                 <  SRR19973067.2655319/150‑1 (MQ=60)
                                                     TCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATT                                                                                                 <  SRR19973067.862842/150‑1 (MQ=60)
                                                      CAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTG                                                                                                <  SRR19973067.640813/150‑1 (MQ=60)
                                                       AAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGA                                                                                               >  SRR19973067.2010332/1‑150 (MQ=60)
                                                       AAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTAA                                                                                               >  SRR19973067.3553661/1‑150 (MQ=60)
                                                         ACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATT                                                                                             >  SRR19973067.3091694/1‑150 (MQ=60)
                                                          CAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTG                                                                                            >  SRR19973067.235773/1‑150 (MQ=60)
                                                             TTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTT                                                                                         <  SRR19973067.2751727/150‑1 (MQ=60)
                                                             TTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTT                                                                                         <  SRR19973067.2751759/150‑1 (MQ=60)
                                                               CCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGT                                                                                       <  SRR19973067.2536639/150‑1 (MQ=60)
                                                               CCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGT                                                                                       >  SRR19973067.570288/1‑150 (MQ=60)
                                                                  CTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAG                                                                                    <  SRR19973067.1216619/150‑1 (MQ=60)
                                                                  CTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAG                                                                                    <  SRR19973067.1970274/150‑1 (MQ=60)
                                                                    GAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCC                                                                                  <  SRR19973067.1409569/150‑1 (MQ=60)
                                                                    GAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCC                                                                                  <  SRR19973067.1409615/150‑1 (MQ=60)
                                                                     AATAGAGCCGATGCCCTGGACAGCGCGTCGTTTTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCA                                                                                 <  SRR19973067.942237/150‑1 (MQ=60)
                                                                     AAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCA                                                                                 >  SRR19973067.8861/1‑150 (MQ=60)
                                                                     AAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCA                                                                                 >  SRR19973067.1620413/1‑150 (MQ=60)
                                                                     AAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCA                                                                                 <  SRR19973067.2731355/150‑1 (MQ=60)
                                                                      AAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCAT                                                                                >  SRR19973067.2880655/1‑150 (MQ=60)
                                                                       AAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATA                                                                               >  SRR19973067.1731375/1‑150 (MQ=60)
                                                                        AGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACCCTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATAT                                                                              <  SRR19973067.1765828/150‑1 (MQ=60)
                                                                        AGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATAT                                                                              >  SRR19973067.2316823/1‑150 (MQ=60)
                                                                        AGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATAT                                                                              >  SRR19973067.2135223/1‑150 (MQ=60)
                                                                        AGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATAT                                                                              >  SRR19973067.2956133/1‑150 (MQ=60)
                                                                        AGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATAACAATTGATTGTTTGTTAGCCATAT                                                                              >  SRR19973067.914025/1‑150 (MQ=60)
                                                                           GCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAG                                                                           >  SRR19973067.3542450/1‑150 (MQ=60)
                                                                           GCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAG                                                                           <  SRR19973067.2432911/150‑1 (MQ=60)
                                                                            CCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGTGCTTGCAGAAAACAAATATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACTATTGATTGTTTGTTATCCATATTAGG                                                                          <  SRR19973067.3531372/150‑1 (MQ=60)
                                                                            CCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGG                                                                          >  SRR19973067.2275785/1‑150 (MQ=60)
                                                                             CGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGC                                                                         >  SRR19973067.1396914/1‑150 (MQ=60)
                                                                              GATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCC                                                                        <  SRR19973067.595896/150‑1 (MQ=60)
                                                                              GATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCC                                                                        <  SRR19973067.295338/150‑1 (MQ=60)
                                                                              GATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCC                                                                        <  SRR19973067.295282/150‑1 (MQ=60)
                                                                              GATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCC                                                                        >  SRR19973067.2146068/1‑150 (MQ=60)
                                                                               ATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCG                                                                       >  SRR19973067.1296473/1‑150 (MQ=60)
                                                                                   CCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGAC                                                                   >  SRR19973067.1509439/1‑150 (MQ=60)
                                                                                   CCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGAC                                                                   >  SRR19973067.1509036/1‑150 (MQ=60)
                                                                                   CCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGGGAC                                                                   >  SRR19973067.1779235/1‑150 (MQ=60)
                                                                                    CTGGACAGCGCGGCGTTGTTCACGGGATGCGTGGTACACTCTTGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTTAAATATATCACAAGTGATTGTTTGTTAGCCATATTAGGCCGTGACT                                                                  >  SRR19973067.2940692/1‑150 (MQ=60)
                                                                                    CTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACT                                                                  <  SRR19973067.3463086/150‑1 (MQ=60)
                                                                                    CTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACT                                                                  <  SRR19973067.3463107/150‑1 (MQ=60)
                                                                                    CTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACT                                                                  <  SRR19973067.3463082/150‑1 (MQ=60)
                                                                                    CTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACT                                                                  >  SRR19973067.2586543/1‑150 (MQ=60)
                                                                                    CTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATGAGGCCGTGACT                                                                  >  SRR19973067.2940913/1‑150 (MQ=60)
                                                                                     TGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGagatcggaagagcacacgtctgaactccagtcacgtattctcgcatctc                                                                 >  SRR19973067.2566237/1‑101 (MQ=60)
                                                                                     TGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTATGCCGTGACTT                                                                 >  SRR19973067.944685/1‑150 (MQ=60)
                                                                                      GGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTT                                                                >  SRR19973067.1990743/1‑150 (MQ=60)
                                                                                          AGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATT                                                            >  SRR19973067.86317/1‑150 (MQ=60)
                                                                                           GCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGCGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTG                                                           <  SRR19973067.901372/150‑1 (MQ=60)
                                                                                                   TTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAG                                                   <  SRR19973067.3330741/150‑1 (MQ=60)
                                                                                                     GTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGAT                                                 <  SRR19973067.2089143/150‑1 (MQ=60)
                                                                                                         ACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATG                                             <  SRR19973067.735538/150‑1 (MQ=60)
                                                                                                         ACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATG                                             <  SRR19973067.735537/150‑1 (MQ=60)
                                                                                                              ATGCGTGGTACACTCTGGCTATCGAGGGTCTTTCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTT                                        <  SRR19973067.3135465/150‑1 (MQ=60)
                                                                                                              AGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTT                                        <  SRR19973067.2629727/150‑1 (MQ=60)
                                                                                                              AGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTT                                        <  SRR19973067.1358157/150‑1 (MQ=60)
                                                                                                              AGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTT                                        <  SRR19973067.779753/150‑1 (MQ=60)
                                                                                                                     GTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGT                                 <  SRR19973067.1834654/150‑1 (MQ=60)
                                                                                                                      TACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTG                                <  SRR19973067.224256/150‑1 (MQ=60)
                                                                                                                      TACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTG                                <  SRR19973067.1433327/150‑1 (MQ=60)
                                                                                                                      TACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTG                                <  SRR19973067.2032872/150‑1 (MQ=60)
                                                                                                                        CACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGC                              >  SRR19973067.66247/1‑150 (MQ=60)
                                                                                                                          CTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCAT                            >  SRR19973067.3450638/1‑150 (MQ=60)
                                                                                                                                CTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGG                      >  SRR19973067.1311767/1‑150 (MQ=60)
                                                                                                                                 TATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGC                     >  SRR19973067.3137826/1‑150 (MQ=60)
                                                                                                                                 TATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGC                     >  SRR19973067.384903/1‑150 (MQ=60)
                                                                                                                                 TATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGC                     >  SRR19973067.384902/1‑150 (MQ=60)
                                                                                                                                          GCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCC            >  SRR19973067.3033804/1‑150 (MQ=60)
                                                                                                                                            TTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCG          <  SRR19973067.312696/150‑1 (MQ=60)
                                                                                                                                             TGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGC         >  SRR19973067.2307358/1‑150 (MQ=60)
                                                                                                                                               CAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCG       <  SRR19973067.1864144/150‑1 (MQ=60)
                                                                                                                                               CAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCG       >  SRR19973067.3133850/1‑150 (MQ=60)
                                                                                                                                                 GAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCC     >  SRR19973067.2169506/1‑150 (MQ=60)
                                                                                                                                                  AAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCT    <  SRR19973067.665554/150‑1 (MQ=60)
                                                                                                                                                    AACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCT  >  SRR19973067.1615000/1‑150 (MQ=60)
                                                                                                                                                    AACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCT  >  SRR19973067.242654/1‑150 (MQ=60)
                                                                                                                                                    AACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCT  >  SRR19973067.3385727/1‑150 (MQ=60)
                                                                                                                                                    AACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCT  <  SRR19973067.1245320/150‑1 (MQ=60)
                                                                                                                                                    AACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGAGAACTATATAACAATTGATTGTTTGTTAGCCATATTAGGCAGTGACTTTTATTGCTCTACAGATTATGTGGTTTTTCAGTGGCATTAAGGTCATATCTTCACGCCGCCTCT  >  SRR19973067.873902/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
GGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCT  >  CP009273/4264916‑4265213

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑