Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F78 I0 R1 626 241.3 10389380 77.7% 8072548 149.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,265,064 C→A 35.0% intergenic (‑95/‑335) yjcB ← / → yjcC putative inner membrane protein/putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,265,0640CA35.0% 377.6 / 253.4 250intergenic (‑95/‑335)yjcB/yjcCputative inner membrane protein/putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base C (82/80);  new base A (37/51);  total (119/131)
Fisher's exact test for biased strand distribution p-value = 2.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 254 total aligned reads displayed.

CACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCT  >  CP009273/4264918‑4265213
                                                                                                                                                  |                                                                                                                                                     
cACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAcaca                                                                                                                                                    >  2:1058146/1‑150 (MQ=255)
  cTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACaaa                                                                                                                                                  >  2:1524912/1‑150 (MQ=255)
  cTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACaaa                                                                                                                                                  <  2:4225707/150‑1 (MQ=255)
  cTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACaaa                                                                                                                                                  >  1:2093030/1‑150 (MQ=255)
   tAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACaaaa                                                                                                                                                 <  2:3198786/150‑1 (MQ=255)
    aagaagTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACaaaaa                                                                                                                                                >  2:2321869/1‑150 (MQ=255)
     agaagTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAAt                                                                                                                                               >  1:600091/1‑150 (MQ=255)
         gTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGaaa                                                                                                                                           <  2:3975583/150‑1 (MQ=255)
          ttGCCAGCGAATAAGAACCTCGCCTGTGGTGAGGGTAGCAATCAATGAATTCCTCTTTAAAGTGCCGTTGGCCTGGACAGCGCGGCGTTGTTCACGGGTGGCGTGGTAAACTCTGGATATCGCGGGGATTGCAGAACACATAATTGTAac                                                                                                                                          <  2:1919471/150‑1 (MQ=255)
          ttGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAac                                                                                                                                          >  2:3041186/1‑150 (MQ=255)
           tGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAaca                                                                                                                                         <  2:2478708/150‑1 (MQ=255)
           tGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTACAGAACACAAAAATGAAaca                                                                                                                                         <  1:2619883/150‑1 (MQ=255)
            gCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAacac                                                                                                                                        <  1:981159/150‑1 (MQ=255)
            gCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAacac                                                                                                                                        <  2:3784372/150‑1 (MQ=255)
            gCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAacac                                                                                                                                        >  1:633638/1‑150 (MQ=255)
                    agaagaaCCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCtgtttg                                                                                                                                >  2:1147877/1‑150 (MQ=255)
                         aaCCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCa                                                                                                                           >  1:2449051/1‑150 (MQ=255)
                         aaCCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCa                                                                                                                           >  2:4443397/1‑150 (MQ=255)
                          aCCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCAt                                                                                                                          >  2:1267352/1‑150 (MQ=255)
                               gCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAAtt                                                                                                                     <  2:2254449/150‑1 (MQ=255)
                               gCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAAtt                                                                                                                     <  1:4335488/150‑1 (MQ=255)
                                ccAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAAttt                                                                                                                    <  2:4951738/150‑1 (MQ=255)
                                 cAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAAtttt                                                                                                                   <  1:2190526/150‑1 (MQ=255)
                                  aGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTtg                                                                                                                  <  1:2809723/150‑1 (MQ=255)
                                    tggtgAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTtgtg                                                                                                                >  1:1948587/1‑150 (MQ=255)
                                     ggtgAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGa                                                                                                               >  1:2543036/1‑150 (MQ=255)
                                          gggTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACtat                                                                                                          <  2:3986972/150‑1 (MQ=255)
                                           ggTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACtata                                                                                                         >  2:5131894/1‑150 (MQ=255)
                                            gTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTatat                                                                                                        <  1:3624495/150‑1 (MQ=255)
                                              aGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATca                                                                                                      <  1:1228680/150‑1 (MQ=255)
                                                  aTCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAat                                                                                                  >  2:788152/1‑150 (MQ=255)
                                                  aTCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAat                                                                                                  >  2:543706/1‑150 (MQ=255)
                                                    cAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAattg                                                                                                >  1:103828/1‑150 (MQ=255)
                                                    cAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAattg                                                                                                <  1:3027061/150‑1 (MQ=255)
                                                    cAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAattg                                                                                                <  1:762847/150‑1 (MQ=255)
                                                       aCAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAattgatt                                                                                             <  2:3741345/150‑1 (MQ=255)
                                                        cAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAattgattg                                                                                            <  1:1267352/150‑1 (MQ=255)
                                                        cAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAattgattg                                                                                            <  2:3630755/150‑1 (MQ=255)
                                                         aagtCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGAttgt                                                                                           <  2:4703032/147‑1 (MQ=255)
                                                         aaTTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGAttgt                                                                                           <  2:612325/150‑1 (MQ=255)
                                                           ttCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGAttgttt                                                                                         <  2:4095165/150‑1 (MQ=255)
                                                             ccTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGAttgtttgt                                                                                       >  1:1484529/1‑150 (MQ=255)
                                                                ctGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAg                                                                                    <  1:4102489/150‑1 (MQ=255)
                                                                  gAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGcc                                                                                  >  1:5042614/1‑150 (MQ=255)
                                                                     aaGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCata                                                                               >  2:3798292/1‑150 (MQ=255)
                                                                     aaGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCata                                                                               <  2:3355512/150‑1 (MQ=255)
                                                                     aaGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCata                                                                               <  2:2145153/150‑1 (MQ=255)
                                                                     aaGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCata                                                                               >  2:1203339/1‑150 (MQ=255)
                                                                        agCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTa                                                                            >  2:187264/1‑150 (MQ=255)
                                                                          ccGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAgg                                                                          >  2:4614381/1‑150 (MQ=255)
                                                                            gATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGcc                                                                        >  1:1296980/1‑150 (MQ=255)
                                                                            gATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGcc                                                                        <  1:1058146/150‑1 (MQ=255)
                                                                                cccTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGATTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGa                                                                    >  1:2906832/1‑150 (MQ=255)
                                                                                cccTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGa                                                                    >  2:4141643/1‑150 (MQ=255)
                                                                                  cTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACt                                                                  <  1:1299640/150‑1 (MQ=255)
                                                                                   tGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACtt                                                                 <  1:4308681/150‑1 (MQ=255)
                                                                                   tGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACtt                                                                 >  2:2830424/1‑150 (MQ=255)
                                                                                   tGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACtt                                                                 <  1:5118505/150‑1 (MQ=255)
                                                                                   tGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACtt                                                                 >  1:5020486/1‑150 (MQ=255)
                                                                                    ggACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACttt                                                                <  2:2040665/150‑1 (MQ=255)
                                                                                    ggACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACttt                                                                <  2:2041383/150‑1 (MQ=255)
                                                                                     gACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACtttt                                                               >  2:3767221/1‑150 (MQ=255)
                                                                                     gACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACtttt                                                               <  1:1541117/150‑1 (MQ=255)
                                                                                     gACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACtttt                                                               <  1:4143194/150‑1 (MQ=255)
                                                                                      aCAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGCTTGTTTGTTAGCCATATTAGGCCGTGACTTTTa                                                              <  2:4709182/150‑1 (MQ=255)
                                                                                           gcggcgTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCt                                                         <  1:1763392/150‑1 (MQ=255)
                                                                                           gcggcgTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGAGTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCt                                                         >  2:3872691/1‑150 (MQ=255)
                                                                                           gcggcgTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTGTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGACGTGACTTTTATTGCt                                                         >  2:2636642/1‑150 (MQ=255)
                                                                                              gcgTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTa                                                      <  2:150778/150‑1 (MQ=255)
                                                                                                  tgttCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGa                                                  <  1:3385338/150‑1 (MQ=255)
                                                                                                    ttCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGAtt                                                >  1:673726/1‑150 (MQ=255)
                                                                                                      cACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTAt                                              <  1:221154/150‑1 (MQ=255)
                                                                                                       aCGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTAtg                                             <  1:4533378/150‑1 (MQ=255)
                                                                                                           gAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGt                                         <  2:1988698/150‑1 (MQ=255)
                                                                                                             ggCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGttt                                       <  1:528292/150‑1 (MQ=255)
                                                                                                                gTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTc                                    >  2:2322911/1‑150 (MQ=255)
                                                                                                                  ggTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAg                                  <  1:237767/150‑1 (MQ=255)
                                                                                                                     acacTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCAGATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTgg                               >  2:3098875/1‑150 (MQ=255)
                                                                                                                     acacTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTgg                               <  2:1172676/150‑1 (MQ=255)
                                                                                                                      cacTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGc                              <  1:840333/150‑1 (MQ=255)
                                                                                                                       acTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTggaa                             >  1:1986262/1‑148 (MQ=255)
                                                                                                                         tctGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCAtt                           >  1:4407045/1‑150 (MQ=255)
                                                                                                                            ggCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAg                        >  1:4000548/1‑150 (MQ=255)
                                                                                                                             gCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAgg                       <  2:1443001/150‑1 (MQ=255)
                                                                                                                             gCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAgg                       <  2:4038744/150‑1 (MQ=255)
                                                                                                                             gCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAgg                       <  1:2922359/150‑1 (MQ=255)
                                                                                                                                aTCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCa                    <  2:617270/150‑1 (MQ=255)
                                                                                                                                aTCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCa                    >  1:466275/1‑150 (MQ=255)
                                                                                                                                     ggggCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCt               <  1:3955824/150‑1 (MQ=255)
                                                                                                                                        gCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTcc            <  1:4340858/150‑1 (MQ=255)
                                                                                                                                         cTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTccc           <  1:3314562/150‑1 (MQ=255)
                                                                                                                                           tGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCccgc         >  1:3250506/1‑150 (MQ=255)
                                                                                                                                           tGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCCTTAAGGGCATATCTTCccgc         >  2:3322270/1‑150 (MQ=255)
                                                                                                                                             cAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAAGTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCccgccg       >  2:4840455/1‑150 (MQ=255)
                                                                                                                                             cAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTATGGGCATATCTTCccgccg       >  1:3775758/1‑150 (MQ=255)
                                                                                                                                              aGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTATGTGAAATATATCACAAATGAATGTTTGTTAGCCATATTAGTCCGTGAATTTTATTGCTGTCCAGAGTAGGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCcgccgc      >  1:4268537/1‑150 (MQ=255)
                                                                                                                                               gAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACACTTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTAGAGATTATGTGGTTTTTCAGTGGCATTAAGGGCCTATCTTCCCgccgcc     >  2:2735880/1‑150 (MQ=255)
                                                                                                                                                aaCACAAAAATGAACCACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCct    <  1:3823572/150‑1 (MQ=255)
                                                                                                                                                aaCACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCct    <  2:2839206/150‑1 (MQ=255)
                                                                                                                                                aaCACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCct    >  2:4414019/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
CACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCT  >  CP009273/4264918‑4265213

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A