Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F86 I0 R1 593 297.4 9515568 94.4% 8982696 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,179,205 G→T 86.4% A24E (GCG→GAG)  rcnR ← transcriptional repressor of rcnA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,179,2050GT86.4% 411.7 / 60.7 153A24E (GCG→GAG) rcnRtranscriptional repressor of rcnA
Reads supporting (aligned to +/- strand):  ref base G (9/12);  new base T (40/92);  total (49/104)
Fisher's exact test for biased strand distribution p-value = 3.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 155 total aligned reads displayed.

GTTTTAGCTCATCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGG  >  CP009273/2179056‑2179354
                                                                                                                                                     |                                                                                                                                                     
      gCTCATCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGa                                                                                                                                                 >  1:3648711/1‑150 (MQ=255)
          aTCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTg                                                                                                                                             >  1:2076902/1‑150 (MQ=255)
          aTCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACGTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTg                                                                                                                                             <  1:3455756/150‑1 (MQ=255)
           tCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTgg                                                                                                                                            >  1:1740014/1‑150 (MQ=255)
            cccccTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGc                                                                                                                                           <  1:3632140/150‑1 (MQ=255)
               ccTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCt                                                                                                                                        <  2:4165841/150‑1 (MQ=255)
               ccTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCt                                                                                                                                        >  2:2485010/1‑150 (MQ=255)
               ccTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCt                                                                                                                                        >  2:2484874/1‑150 (MQ=255)
               ccTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCt                                                                                                                                        >  2:2556219/1‑150 (MQ=255)
                cTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTg                                                                                                                                       <  1:2559072/150‑1 (MQ=255)
                cTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTg                                                                                                                                       >  2:596180/1‑150 (MQ=255)
                 tGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCCGa                                                                                                                                      <  2:1481598/150‑1 (MQ=255)
                   gTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAAt                                                                                                                                    >  2:2106424/1‑150 (MQ=255)
                      aaCGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCtt                                                                                                                                 <  2:1989977/150‑1 (MQ=255)
                       aCGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTa                                                                                                                                <  1:2403153/150‑1 (MQ=255)
                           tgtgTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTgg                                                                                                                            <  1:3949676/150‑1 (MQ=255)
                            gtgtTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGc                                                                                                                           <  1:1309631/150‑1 (MQ=255)
                               ttCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCAcg                                                                                                                        <  1:3331923/150‑1 (MQ=255)
                                 ccGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCAcgcg                                                                                                                      >  1:207552/1‑150 (MQ=255)
                                 ccGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCAcgcg                                                                                                                      <  2:3002720/150‑1 (MQ=255)
                                       gATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCa                                                                                                                <  2:2123242/150‑1 (MQ=255)
                                       gATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCa                                                                                                                <  1:4303008/150‑1 (MQ=255)
                                        aTGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAg                                                                                                               <  1:3307234/150‑1 (MQ=255)
                                        aTGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAg                                                                                                               <  1:4095593/150‑1 (MQ=255)
                                          gACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGtt                                                                                                             <  1:301990/150‑1 (MQ=255)
                                                 aaTCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGttt                                                                                                      <  1:513442/150‑1 (MQ=255)
                                                    cACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATc                                                                                                   <  2:807093/150‑1 (MQ=255)
                                                     aCTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCa                                                                                                  >  1:3930533/1‑150 (MQ=255)
                                                     aCTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCa                                                                                                  <  1:1193215/150‑1 (MQ=255)
                                                      cTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCAc                                                                                                 <  1:886720/150‑1 (MQ=255)
                                                      cTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCAc                                                                                                 <  1:3257841/150‑1 (MQ=255)
                                                              aTCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTa                                                                                         >  2:4449220/1‑150 (MQ=255)
                                                               tCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTAt                                                                                        <  1:1888935/150‑1 (MQ=255)
                                                               tCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGAGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTAt                                                                                        >  2:2162188/1‑150 (MQ=255)
                                                                 aGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATga                                                                                      >  1:3564598/1‑150 (MQ=255)
                                                                 aGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGAGTGTATga                                                                                      >  2:2850607/1‑150 (MQ=255)
                                                                    ccGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGAc                                                                                   >  1:563271/1‑150 (MQ=255)
                                                                     cGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACa                                                                                  <  1:4124472/150‑1 (MQ=255)
                                                                       tttACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATg                                                                                >  1:3561784/1‑150 (MQ=255)
                                                                        ttACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCAGTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATgg                                                                               <  1:2780538/150‑1 (MQ=255)
                                                                           ccGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCaa                                                                            <  2:3981595/150‑1 (MQ=255)
                                                                            cgcgCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAac                                                                           <  2:2049482/150‑1 (MQ=255)
                                                                                ccACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCt                                                                       <  2:3649929/150‑1 (MQ=255)
                                                                                ccACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCt                                                                       >  1:1094822/1‑150 (MQ=255)
                                                                                ccACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCt                                                                       <  1:172323/150‑1 (MQ=255)
                                                                                 cACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTg                                                                      <  2:2596713/150‑1 (MQ=255)
                                                                                 cACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTg                                                                      <  1:4604241/150‑1 (MQ=255)
                                                                                  aCGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTgg                                                                     <  2:481971/150‑1 (MQ=255)
                                                                                    ggATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGtt                                                                   <  2:1412866/150‑1 (MQ=255)
                                                                                     gATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTa                                                                  >  1:2104144/1‑150 (MQ=255)
                                                                                      aTAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTaa                                                                 <  1:3111339/150‑1 (MQ=255)
                                                                                      aTAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTaa                                                                 <  1:3842094/150‑1 (MQ=255)
                                                                                         gcagcaATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACaa                                                              <  2:3066669/150‑1 (MQ=255)
                                                                                          cagcaATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGTCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAg                                                             <  1:2990712/150‑1 (MQ=255)
                                                                                          cagcaATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAg                                                             <  1:2990882/150‑1 (MQ=255)
                                                                                           agcaATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGa                                                            <  1:4519550/150‑1 (MQ=255)
                                                                                            gcaATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGaa                                                           <  2:3140392/150‑1 (MQ=255)
                                                                                            gcaATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGaa                                                           <  2:3139631/150‑1 (MQ=255)
                                                                                             caATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAat                                                          <  2:2466754/150‑1 (MQ=255)
                                                                                             caATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAat                                                          <  1:4166620/150‑1 (MQ=255)
                                                                                             caATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAat                                                          <  1:1780266/150‑1 (MQ=255)
                                                                                             caATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAat                                                          >  2:4135656/1‑150 (MQ=255)
                                                                                               aTCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAatat                                                        <  1:218008/150‑1 (MQ=255)
                                                                                                 cTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGa                                                      >  1:2935251/1‑150 (MQ=255)
                                                                                                  tgttgtAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGaa                                                     >  1:3458733/1‑150 (MQ=255)
                                                                                                   gttgtAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGaaa                                                    <  1:177964/150‑1 (MQ=255)
                                                                                                    ttgtAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGaaaa                                                   >  1:4320571/1‑150 (MQ=255)
                                                                                                     tgtAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGaaaaa                                                  <  1:2184508/150‑1 (MQ=255)
                                                                                                       tAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATc                                                <  2:3183875/150‑1 (MQ=255)
                                                                                                        aaatCTGCAGCGCAGTCGTGCGGCTCGTCGATCATTTTCTTGAGCTCCACGACCTGGCCATGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAATTCa                                               <  2:2773718/150‑1 (MQ=255)
                                                                                                        aaaaCTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCa                                               >  1:2511216/1‑150 (MQ=255)
                                                                                                        aaaaCTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCa                                               <  2:2802894/150‑1 (MQ=255)
                                                                                                          aaCTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATa                                             <  2:4542506/150‑1 (MQ=255)
                                                                                                           aCTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAg                                            <  2:3082491/150‑1 (MQ=255)
                                                                                                           aCTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAg                                            >  1:3119707/1‑150 (MQ=255)
                                                                                                              gCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCAc                                         <  2:3652906/150‑1 (MQ=255)
                                                                                                               cAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACt                                        >  1:507783/1‑150 (MQ=255)
                                                                                                               cAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACt                                        <  2:1818468/150‑1 (MQ=255)
                                                                                                               cAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACt                                        <  1:437000/150‑1 (MQ=255)
                                                                                                                aGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTa                                       <  1:1473875/150‑1 (MQ=255)
                                                                                                                aGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTa                                       <  1:1473511/150‑1 (MQ=255)
                                                                                                                  cgcATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTAtt                                     <  2:1740014/150‑1 (MQ=255)
                                                                                                                  cgcATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTAtt                                     <  1:660847/150‑1 (MQ=255)
                                                                                                                  cgcATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTAtt                                     >  1:2950544/1‑150 (MQ=255)
                                                                                                                    cATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTaa                                   <  1:2277861/150‑1 (MQ=255)
                                                                                                                     aTTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAAt                                  <  2:607959/150‑1 (MQ=255)
                                                                                                                       tCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCt                                <  2:849783/150‑1 (MQ=255)
                                                                                                                       tCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCt                                <  2:850172/150‑1 (MQ=255)
                                                                                                                       tCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCt                                <  2:3865607/150‑1 (MQ=255)
                                                                                                                       tCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCt                                <  1:228572/150‑1 (MQ=255)
                                                                                                                          tGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACt                             <  1:2556219/150‑1 (MQ=255)
                                                                                                                          tGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACt                             <  1:2556206/150‑1 (MQ=255)
                                                                                                                           gCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTg                            <  1:2106424/150‑1 (MQ=255)
                                                                                                                                    cgAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGgtagt                   <  1:596180/150‑1 (MQ=255)
                                                                                                                                     gAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGgtagtc                  >  2:115446/1‑149 (MQ=255)
                                                                                                                                      aGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAACAATCATAGCACTATTAATCTACTGGGGGGTCGTAt                 >  2:2384429/1‑150 (MQ=255)
                                                                                                                                      aGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTAt                 <  1:3771946/150‑1 (MQ=255)
                                                                                                                                         aTTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAg              >  1:1158580/1‑150 (MQ=255)
                                                                                                                                             tCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTAc          <  1:2485010/150‑1 (MQ=255)
                                                                                                                                              cTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACt         >  1:421884/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GTTTTAGCTCATCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGG  >  CP009273/2179056‑2179354

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTTTTAGCTCATCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGG  >  CP009273/2179056‑2179354
                                                                                                                                                     |                                                                                                                                                     
GTTTTAGCTCATCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCG                                                                                                                                                       >  SRR19973082.4156538/1‑150 (MQ=60)
 TTTTAGCTCATCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTC                                                                                                                                                      <  SRR19973082.598439/150‑1 (MQ=60)
      GCTCATCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGA                                                                                                                                                 >  SRR19973082.3671195/1‑150 (MQ=60)
      GCTCATCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGA                                                                                                                                                 >  SRR19973082.3672422/1‑150 (MQ=60)
       CTCATCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGAC                                                                                                                                                >  SRR19973082.1900782/1‑150 (MQ=60)
          ATCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTG                                                                                                                                             <  SRR19973082.2185189/150‑1 (MQ=60)
          ATCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTG                                                                                                                                             >  SRR19973082.2090125/1‑150 (MQ=60)
          ATCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTG                                                                                                                                             >  SRR19973082.881982/1‑150 (MQ=60)
          ATCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTG                                                                                                                                             <  SRR19973082.3477196/150‑1 (MQ=60)
          ATCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACGTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTG                                                                                                                                             <  SRR19973082.3477005/150‑1 (MQ=60)
           TCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGG                                                                                                                                            >  SRR19973082.1751552/1‑150 (MQ=60)
           TCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGG                                                                                                                                            >  SRR19973082.3364448/1‑150 (MQ=60)
            CCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGC                                                                                                                                           <  SRR19973082.3654472/150‑1 (MQ=60)
                CTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTG                                                                                                                                       <  SRR19973082.2575036/150‑1 (MQ=60)
                CTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGGTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTG                                                                                                                                       <  SRR19973082.2574633/150‑1 (MQ=60)
                 TGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGA                                                                                                                                      <  SRR19973082.3653558/150‑1 (MQ=60)
                   GTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAATCTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAAT                                                                                                                                    <  SRR19973082.2551287/150‑1 (MQ=60)
                       ACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTA                                                                                                                                <  SRR19973082.2418014/150‑1 (MQ=60)
                       ACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTA                                                                                                                                <  SRR19973082.1971969/150‑1 (MQ=60)
                           TGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGG                                                                                                                            <  SRR19973082.3973678/150‑1 (MQ=60)
                            GTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGC                                                                                                                           <  SRR19973082.4266927/150‑1 (MQ=60)
                            GTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGC                                                                                                                           <  SRR19973082.1318681/150‑1 (MQ=60)
                               TTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACG                                                                                                                        <  SRR19973082.3352535/150‑1 (MQ=60)
                               TTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACG                                                                                                                        <  SRR19973082.3349781/150‑1 (MQ=60)
                                 CCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCG                                                                                                                      >  SRR19973082.209255/1‑150 (MQ=60)
                                       GATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCA                                                                                                                <  SRR19973082.4329118/150‑1 (MQ=60)
                                       GATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCA                                                                                                                <  SRR19973082.4329024/150‑1 (MQ=60)
                                        ATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAG                                                                                                               <  SRR19973082.3327674/150‑1 (MQ=60)
                                        ATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAG                                                                                                               <  SRR19973082.4120685/150‑1 (MQ=60)
                                         TGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGT                                                                                                              <  SRR19973082.1846899/150‑1 (MQ=60)
                                          GACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTT                                                                                                             <  SRR19973082.304142/150‑1 (MQ=60)
                                               TTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCCTTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTCCTGGCACGCGCTGTCAGTTTCTGT                                                                                                        >  SRR19973082.208825/1‑150 (MQ=60)
                                                 AATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTT                                                                                                      <  SRR19973082.517103/150‑1 (MQ=60)
                                                 AATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTT                                                                                                      <  SRR19973082.517283/150‑1 (MQ=60)
                                                     ACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCA                                                                                                  <  SRR19973082.1200693/150‑1 (MQ=60)
                                                     ACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCA                                                                                                  <  SRR19973082.1201171/150‑1 (MQ=60)
                                                     ACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCA                                                                                                  >  SRR19973082.3954465/1‑150 (MQ=60)
                                                      CTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCAC                                                                                                 <  SRR19973082.3277885/150‑1 (MQ=60)
                                                      CTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCAC                                                                                                 <  SRR19973082.892826/150‑1 (MQ=60)
                                                            GCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTG                                                                                           >  SRR19973082.4619737/1‑150 (MQ=60)
                                                               TCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTAT                                                                                        <  SRR19973082.1901075/150‑1 (MQ=60)
                                                                 AGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGA                                                                                      >  SRR19973082.3584922/1‑150 (MQ=60)
                                                                 AGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGA                                                                                      >  SRR19973082.3586201/1‑150 (MQ=60)
                                                                    CCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGAC                                                                                   >  SRR19973082.567298/1‑150 (MQ=60)
                                                                     CGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACA                                                                                  <  SRR19973082.4149735/150‑1 (MQ=60)
                                                                       TTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATG                                                                                >  SRR19973082.3583374/1‑150 (MQ=60)
                                                                        TGACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCAGTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGG                                                                               <  SRR19973082.2797340/150‑1 (MQ=60)
                                                                            CGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAAC                                                                           >  SRR19973082.2831935/1‑150 (MQ=60)
                                                                                CCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCT                                                                       <  SRR19973082.173798/150‑1 (MQ=60)
                                                                                CCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCT                                                                       >  SRR19973082.1102246/1‑150 (MQ=60)
                                                                                 CACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTG                                                                      <  SRR19973082.4632193/150‑1 (MQ=60)
                                                                                 CACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTG                                                                      <  SRR19973082.1636324/150‑1 (MQ=60)
                                                                                    GGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTT                                                                   >  SRR19973082.3125714/1‑150 (MQ=60)
                                                                                     GATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTA                                                                  <  SRR19973082.2218213/150‑1 (MQ=60)
                                                                                     GATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTA                                                                  >  SRR19973082.2117540/1‑150 (MQ=60)
                                                                                      ATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAA                                                                 <  SRR19973082.3130266/150‑1 (MQ=60)
                                                                                      ATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAA                                                                 <  SRR19973082.3865722/150‑1 (MQ=60)
                                                                                          CAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGTCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAG                                                             <  SRR19973082.3009166/150‑1 (MQ=60)
                                                                                          CAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAG                                                             <  SRR19973082.3009336/150‑1 (MQ=60)
                                                                                          CAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAG                                                             <  SRR19973082.3008997/150‑1 (MQ=60)
                                                                                           AGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGA                                                            >  SRR19973082.3019997/1‑150 (MQ=60)
                                                                                           AGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGA                                                            <  SRR19973082.4547046/150‑1 (MQ=60)
                                                                                           AGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGA                                                            <  SRR19973082.4546961/150‑1 (MQ=60)
                                                                                             CAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAAT                                                          <  SRR19973082.1792004/150‑1 (MQ=60)
                                                                                             CAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAAT                                                          <  SRR19973082.4192123/150‑1 (MQ=60)
                                                                                               ATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATAT                                                        <  SRR19973082.219770/150‑1 (MQ=60)
                                                                                                 CTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGA                                                      >  SRR19973082.2953392/1‑150 (MQ=60)
                                                                                                  TGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAA                                                     >  SRR19973082.3479992/1‑150 (MQ=60)
                                                                                                   GTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAA                                                    <  SRR19973082.179478/150‑1 (MQ=60)
                                                                                                    TTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAA                                                   >  SRR19973082.4346647/1‑150 (MQ=60)
                                                                                                     TGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAA                                                  <  SRR19973082.2198361/150‑1 (MQ=60)
                                                                                                        AAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCA                                               >  SRR19973082.2526889/1‑150 (MQ=60)
                                                                                                         AAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCAT                                              <  SRR19973082.4041097/150‑1 (MQ=60)
                                                                                                           ACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAG                                            >  SRR19973082.3138647/1‑150 (MQ=60)
                                                                                                           ACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAG                                            >  SRR19973082.3868552/1‑150 (MQ=60)
                                                                                                               CAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACT                                        >  SRR19973082.511399/1‑150 (MQ=60)
                                                                                                               CAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACT                                        >  SRR19973082.511115/1‑150 (MQ=60)
                                                                                                               CAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACT                                        <  SRR19973082.439991/150‑1 (MQ=60)
                                                                                                                AGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTA                                       <  SRR19973082.1483429/150‑1 (MQ=60)
                                                                                                                AGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTA                                       <  SRR19973082.1483796/150‑1 (MQ=60)
                                                                                                                  CGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATT                                     <  SRR19973082.665406/150‑1 (MQ=60)
                                                                                                                  CGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATT                                     >  SRR19973082.2968768/1‑150 (MQ=60)
                                                                                                                    CATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAA                                   <  SRR19973082.2292098/150‑1 (MQ=60)
                                                                                                                     ATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAAT                                  <  SRR19973082.2343444/150‑1 (MQ=60)
                                                                                                                       TCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCT                                <  SRR19973082.1359083/150‑1 (MQ=60)
                                                                                                                       TCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCT                                <  SRR19973082.230379/150‑1 (MQ=60)
                                                                                                                          TGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACT                             <  SRR19973082.2572154/150‑1 (MQ=60)
                                                                                                                          TGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACT                             <  SRR19973082.2572167/150‑1 (MQ=60)
                                                                                                                           GCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTG                            <  SRR19973082.2119834/150‑1 (MQ=60)
                                                                                                                                  GTCGAGCATTTTTTTGAGTTGCTCGACCTGGCCTTGATTCTTACTGGCACGCGATTTAAGTTTCTGTTTATGACGTATTGTATGAGTCATGGCAATACCTGGTTTAAAAGTTTATGAAAAATCATAGCACTATTTATCTACTGGGGGGTA                     <  SRR19973082.2110725/150‑1 (MQ=56)
                                                                                                                                   TCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAG                    <  SRR19973082.635814/150‑1 (MQ=60)
                                                                                                                                    CGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGT                   <  SRR19973082.600421/150‑1 (MQ=60)
                                                                                                                                    CGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGT                   >  SRR19973082.3167369/1‑150 (MQ=60)
                                                                                                                                      AGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTAT                 <  SRR19973082.3795241/150‑1 (MQ=60)
                                                                                                                                      AGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTAT                 >  SRR19973082.1782043/1‑150 (MQ=60)
                                                                                                                                         ATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAG              >  SRR19973082.1166197/1‑150 (MQ=60)
                                                                                                                                         ATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAG              >  SRR19973082.3574671/1‑150 (MQ=60)
                                                                                                                                             TCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTAC          <  SRR19973082.2500524/150‑1 (MQ=60)
                                                                                                                                             TCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTAC          <  SRR19973082.2500388/150‑1 (MQ=60)
                                                                                                                                              CTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACT         >  SRR19973082.424689/1‑150 (MQ=60)
                                                                                                                                                     TCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGG  <  SRR19973082.2520952/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
GTTTTAGCTCATCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGG  >  CP009273/2179056‑2179354

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑