Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F98 I0 R1	799	294.1	9752082	92.2%	8991419	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,179,205	G→T	66.3%	A24E (GCG→GAG)	rcnR ←	transcriptional repressor of rcnA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,179,205	0	G	T	66.3%	182.3 / 207.0	172	A24E (GCG→GAG)	rcnR	transcriptional repressor of rcnA
Reads supporting (aligned to +/- strand): ref base G (26/32); new base T (50/64); total (76/96)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 173 total aligned reads displayed.

TTTAGCTCATCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGG  >  CP009273/2179058‑2179352

tttAGCTCATCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGcc                                                                                                                                                   >  2:2875946/1‑150 (MQ=255)

   aGCTCATCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCAca                                                                                                                                                <  1:3982536/150‑2 (MQ=255)

    gCTCATCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGa                                                                                                                                               >  1:3209037/1‑150 (MQ=255)

      tCATCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGAcc                                                                                                                                             >  1:4556573/1‑150 (MQ=255)

       cATCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCt                                                                                                                                            <  1:3604395/150‑1 (MQ=255)

         tCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTgg                                                                                                                                          <  1:4680704/150‑1 (MQ=255)

         tCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTgg                                                                                                                                          >  1:157155/1‑150 (MQ=255)

          cccccTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGc                                                                                                                                         <  1:1104758/150‑1 (MQ=255)

            cccTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGccc                                                                                                                                       >  1:1320157/1‑150 (MQ=255)

            cccTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGccc                                                                                                                                       >  2:3197754/1‑150 (MQ=255)

                   gAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCt                                                                                                                                <  1:1720468/150‑1 (MQ=255)

                    aaCGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCtt                                                                                                                               <  1:91858/150‑1 (MQ=255)

                       gATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACt                                                                                                                            <  2:3027797/150‑1 (MQ=255)

                           tgtTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCa                                                                                                                        >  2:154650/1‑150 (MQ=255)

                           tgtTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCa                                                                                                                        >  1:3619347/1‑150 (MQ=255)

                            gtTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCAc                                                                                                                       <  2:1997408/150‑1 (MQ=255)

                                  tCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCtt                                                                                                                 >  1:4334488/1‑150 (MQ=255)

                                    aGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTc                                                                                                               <  1:3389285/150‑1 (MQ=255)

                                     gATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCa                                                                                                              <  1:4395248/150‑1 (MQ=255)

                                      aTGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAg                                                                                                             >  1:2908718/1‑150 (MQ=255)

                                          ccTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTc                                                                                                         >  1:2420513/1‑150 (MQ=255)

                                            tttAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTg                                                                                                       <  2:1109781/150‑1 (MQ=255)

                                            tttAATCACTACCCGCATCAGACCGTATACCGCGCCACGGATAGCAGCAATCTGTAGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTg                                                                                                       >  2:3512369/1‑150 (MQ=255)

                                              tAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGtt                                                                                                     <  2:3869549/150‑1 (MQ=255)

                                              tAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGtt                                                                                                     <  2:663253/150‑1 (MQ=255)

                                              tAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGtt                                                                                                     >  2:254169/1‑150 (MQ=255)

                                               aaTCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGttt                                                                                                    <  2:3250529/150‑1 (MQ=255)

                                                aTCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTa                                                                                                   <  1:2106579/150‑1 (MQ=255)

                                                 tCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTAt                                                                                                  >  2:2309438/1‑150 (MQ=255)

                                                  cACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATc                                                                                                 <  1:1247883/150‑1 (MQ=255)

                                                   aCTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCa                                                                                                >  1:4256152/1‑150 (MQ=255)

                                                    cTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCAc                                                                                               >  1:2467186/1‑150 (MQ=255)

                                                      tCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACgg                                                                                             >  1:1423760/1‑150 (MQ=255)

                                                      tCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACgg                                                                                             >  1:1423770/1‑150 (MQ=255)

                                                      tCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTGCAGTTTCTGTTTATCACgg                                                                                             >  2:1274881/1‑150 (MQ=255)

                                                       cccGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGa                                                                                            >  1:1943351/1‑150 (MQ=255)

                                                           cATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGt                                                                                        <  1:4392608/150‑1 (MQ=255)

                                                            aTCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTa                                                                                       <  1:154650/150‑1 (MQ=255)

                                                             tCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTAt                                                                                      <  1:1267338/150‑1 (MQ=255)

                                                                  ccatttACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGAc                                                                                 >  1:3459498/4‑150 (MQ=255)

                                                                  ccGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGAc                                                                                 >  1:3450721/1‑150 (MQ=255)

                                                                  ccGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGAc                                                                                 <  2:4113629/150‑1 (MQ=255)

                                                                    gTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACAt                                                                               <  2:4393010/150‑1 (MQ=255)

                                                                      ttACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATgg                                                                             <  1:1380008/150‑1 (MQ=255)

                                                                      ttACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATgg                                                                             >  1:2474873/1‑150 (MQ=255)

                                                                      ttACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATgg                                                                             <  2:629458/150‑1 (MQ=255)

                                                                              ccACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCt                                                                     <  1:3362636/150‑1 (MQ=255)

                                                                                 cGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGt                                                                  >  1:1517479/1‑150 (MQ=255)

                                                                                  tgATATCAGCTATCTGTTGTAATACTGCAGCGCATTCGTGCGGCTCGTCGAGGATTTTCTTGAGCTCCTCGACCTGGCCCTGAATCTTACTGGCACGCTCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGtt                                                                 <  2:2731230/149‑1 (MQ=255)

                                                                                  ggATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGtt                                                                 >  2:250259/1‑150 (MQ=255)

                                                                                   gATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTa                                                                >  1:553914/1‑150 (MQ=255)

                                                                                    aTAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGAGGCTCGTCGAGCATTTTCTTGATCTCCAAGACCTGGCCCTGAATCTTACTGGCACGCGAATTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGAAACACCTTGTTaa                                                               >  2:812068/1‑150 (MQ=255)

                                                                                      agcagcAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACa                                                             <  2:427412/150‑1 (MQ=255)

                                                                                           caATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAat                                                        >  1:3435119/1‑150 (MQ=255)

                                                                                            aaTCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAata                                                       >  1:4589362/1‑150 (MQ=255)

                                                                                            aaTCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAata                                                       <  1:3197754/150‑1 (MQ=255)

                                                                                             aTCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAatat                                                      <  2:4334488/150‑1 (MQ=255)

                                                                                             aTCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAatat                                                      >  1:2678909/1‑150 (MQ=255)

                                                                                                tgttgtAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGaa                                                   >  2:1435344/1‑150 (MQ=255)

                                                                                                tgttgtAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGaa                                                   >  2:3896108/1‑150 (MQ=255)

                                                                                                    gtAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAAt                                               <  2:2377955/150‑1 (MQ=255)

                                                                                                     tAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATc                                              <  1:3861509/150‑1 (MQ=255)

                                                                                                     tAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATc                                              >  2:1279214/1‑150 (MQ=255)

                                                                                                       aaaCTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCAt                                            >  1:2237657/1‑150 (MQ=255)

                                                                                                        aaCTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATa                                           <  2:4256152/150‑1 (MQ=255)

                                                                                                        aaCTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATa                                           <  2:1321052/150‑1 (MQ=255)

                                                                                                          cTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTGTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGc                                         <  1:4393149/150‑1 (MQ=255)

                                                                                                           tGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCa                                        <  1:2373709/150‑1 (MQ=255)

                                                                                                             cAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACt                                      >  1:3358195/1‑150 (MQ=255)

                                                                                                             cAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACt                                      <  1:100540/150‑1 (MQ=255)

                                                                                                             cAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACt                                      >  1:4214462/1‑150 (MQ=255)

                                                                                                              aGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTa                                     >  1:1874764/1‑150 (MQ=255)

                                                                                                                 gcATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTa                                  <  2:1898899/150‑1 (MQ=255)

                                                                                                                   aTTCGTGCGTCTAGTCGATCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGTCACGCTCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACTTGTCATCACCTGGTTAACATTTATATGAAAATTCATAGCACTATTAAt                                <  1:3807096/150‑1 (MQ=255)

                                                                                                                     tCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCt                              <  1:3261189/150‑1 (MQ=255)

                                                                                                                     tCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCt                              <  2:3227667/150‑1 (MQ=255)

                                                                                                                     tCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCt                              <  2:4710487/150‑1 (MQ=255)

                                                                                                                      cGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTa                             >  1:2491641/1‑150 (MQ=255)

                                                                                                                       gTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTAc                            >  1:713700/1‑150 (MQ=255)

                                                                                                                       gTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTGTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATCTGAAAAATCATAGCACTATTAATCGAc                            >  2:4315912/1‑150 (MQ=255)

                                                                                                                        tGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACt                           >  1:2361632/1‑150 (MQ=255)

                                                                                                                          cGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTgg                         <  2:1530324/150‑1 (MQ=255)

                                                                                                                          cGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTgg                         <  2:220719/150‑1 (MQ=255)

                                                                                                                                 tcgAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGgtag                  <  2:4041678/150‑1 (MQ=255)

                                                                                                                                  cgAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGgtagt                 <  2:4021052/150‑1 (MQ=255)

                                                                                                                                  cgAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGgtagt                 >  1:217480/1‑150 (MQ=255)

                                                                                                                                   gAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGgtagta                <  2:1868834/150‑1 (MQ=255)

                                                                                                                                   gAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGgtagta                <  2:2061133/150‑1 (MQ=255)

                                                                                                                                     gCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATc              <  2:3823251/150‑1 (MQ=255)

                                                                                                                                      cATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCa             <  1:1360716/150‑1 (MQ=255)

                                                                                                                                       aTTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAg            <  2:3530414/150‑1 (MQ=255)

                                                                                                                                       aTTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAg            <  2:70611/150‑1 (MQ=255)

                                                                                                                                       aTTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAg            <  2:70685/150‑1 (MQ=255)

                                                                                                                                         tttCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGt          >  2:3146310/1‑150 (MQ=255)

                                                                                                                                         tttCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGt          <  2:368505/150‑1 (MQ=255)

                                                                                                                                            cTTGATCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTAAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAACTCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACt       >  2:4642405/1‑150 (MQ=255)

                                                                                                                                            cTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCCGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTCCt       >  2:2190227/1‑150 (MQ=255)

                                                                                                                                             ttGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTg      <  1:873064/150‑1 (MQ=255)

                                                                                                                                             ttGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTg      <  2:157155/150‑1 (MQ=255)

                                                                                                                                                 gcgcCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTggggg  <  1:4295124/150‑1 (MQ=255)

TTTAGCTCATCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGG  >  CP009273/2179058‑2179352

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TTAGCTCATCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGG  >  CP009273/2179059‑2179352

TTAGCTCATCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCA                                                                                                                                                  >  SRR19973081.1455803/1‑150 (MQ=60)

  AGCTCATCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACA                                                                                                                                                <  SRR19973081.4006807/150‑1 (MQ=60)

  AGCTCATCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACA                                                                                                                                                <  SRR19973081.4006689/150‑1 (MQ=60)

   GCTCATCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGA                                                                                                                                               >  SRR19973081.3228440/1‑150 (MQ=60)

     TCATCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACC                                                                                                                                             >  SRR19973081.4584200/1‑150 (MQ=60)

     TCATCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACC                                                                                                                                             >  SRR19973081.4584193/1‑150 (MQ=60)

      CATCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCT                                                                                                                                            <  SRR19973081.3626233/150‑1 (MQ=60)

        TCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGG                                                                                                                                          <  SRR19973081.4709106/150‑1 (MQ=60)

        TCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGG                                                                                                                                          >  SRR19973081.4178668/1‑150 (MQ=60)

        TCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGG                                                                                                                                          >  SRR19973081.157867/1‑150 (MQ=60)

        TCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGG                                                                                                                                          >  SRR19973081.158608/1‑150 (MQ=60)

         CCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGC                                                                                                                                         >  SRR19973081.4045437/1‑150 (MQ=60)

         CCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGC                                                                                                                                         <  SRR19973081.1112031/150‑1 (MQ=60)

           CCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCC                                                                                                                                       >  SRR19973081.1329892/1‑150 (MQ=60)

           CCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCC                                                                                                                                       >  SRR19973081.1328991/1‑150 (MQ=60)

                  GAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCT                                                                                                                                <  SRR19973081.1731749/150‑1 (MQ=60)

                   AACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTT                                                                                                                               <  SRR19973081.92843/150‑1 (MQ=60)

                   AACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTT                                                                                                                               <  SRR19973081.92823/150‑1 (MQ=60)

                       ATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTG                                                                                                                           >  SRR19973081.2591462/1‑150 (MQ=60)

                       ATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTG                                                                                                                           >  SRR19973081.2591461/1‑150 (MQ=60)

                          TGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCA                                                                                                                        >  SRR19973081.3641233/1‑150 (MQ=60)

                                 TCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTT                                                                                                                 >  SRR19973081.4360801/1‑150 (MQ=60)

                                   AGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTC                                                                                                               <  SRR19973081.3410265/150‑1 (MQ=60)

                                   AGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTC                                                                                                               <  SRR19973081.3410125/150‑1 (MQ=60)

                                    GATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCA                                                                                                              <  SRR19973081.4421766/150‑1 (MQ=60)

                                     ATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAG                                                                                                             <  SRR19973081.638644/150‑1 (MQ=60)

                                     ATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAG                                                                                                             >  SRR19973081.2926636/1‑150 (MQ=60)

                                         CCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTC                                                                                                         >  SRR19973081.2435366/1‑150 (MQ=60)

                                           TTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTG                                                                                                       >  SRR19973081.2466105/1‑150 (MQ=60)

                                             TAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTT                                                                                                     <  SRR19973081.1269938/150‑1 (MQ=60)

                                               ATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTA                                                                                                   <  SRR19973081.4586574/150‑1 (MQ=60)

                                               ATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTA                                                                                                   <  SRR19973081.2119988/150‑1 (MQ=60)

                                                 CACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATC                                                                                                 <  SRR19973081.1256098/150‑1 (MQ=60)

                                                 CACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATC                                                                                                 <  SRR19973081.4288751/150‑1 (MQ=60)

                                                  ACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCA                                                                                                >  SRR19973081.4282131/1‑150 (MQ=60)

                                                   CTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCAC                                                                                               >  SRR19973081.2482498/1‑150 (MQ=60)

                                                     TCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGG                                                                                             >  SRR19973081.1433287/1‑150 (MQ=60)

                                                     TCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGG                                                                                             >  SRR19973081.1433297/1‑150 (MQ=60)

                                                      CCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGA                                                                                            >  SRR19973081.1955672/1‑150 (MQ=60)

                                                        CGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATT                                                                                          >  SRR19973081.2392681/1‑150 (MQ=60)

                                                          CATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGT                                                                                        <  SRR19973081.4419114/150‑1 (MQ=60)

                                                           ATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTA                                                                                       <  SRR19973081.156092/150‑1 (MQ=60)

                                                           ATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTA                                                                                       >  SRR19973081.3035036/1‑150 (MQ=60)

                                                           ATCAGACCGTGTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTT                                                                                       <  SRR19973081.2606296/150‑1 (MQ=60)

                                                            TCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTAT                                                                                      <  SRR19973081.1275718/150‑1 (MQ=60)

                                                             CAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATG                                                                                     >  SRR19973081.39946/1‑150 (MQ=60)

                                                             CAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATG                                                                                     >  SRR19973081.40079/1‑150 (MQ=60)

                                                              AGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGA                                                                                    >  SRR19973081.4739022/1‑150 (MQ=60)

                                                                 CCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGAC                                                                                 >  SRR19973081.3471946/1‑150 (MQ=60)

                                                                 CCATTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGAC                                                                                 >  SRR19973081.3480763/1‑150 (MQ=60)

                                                                   GTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACAT                                                                               <  SRR19973081.2373376/150‑1 (MQ=60)

                                                                     TTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGG                                                                             <  SRR19973081.1389268/150‑1 (MQ=60)

                                                                     TTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGG                                                                             >  SRR19973081.2490012/1‑150 (MQ=60)

                                                                     TTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGG                                                                             <  SRR19973081.1389260/150‑1 (MQ=60)

                                                                     TTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGG                                                                             >  SRR19973081.2490245/1‑150 (MQ=60)

                                                                          GCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACA                                                                        <  SRR19973081.3226244/150‑1 (MQ=60)

                                                                             CCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCT                                                                     <  SRR19973081.3383280/150‑1 (MQ=60)

                                                                             CCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCT                                                                     <  SRR19973081.3383279/150‑1 (MQ=60)

                                                                                CGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGT                                                                  <  SRR19973081.4374736/150‑1 (MQ=60)

                                                                                CGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGT                                                                  >  SRR19973081.1527452/1‑150 (MQ=60)

                                                                                  GATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTA                                                                >  SRR19973081.557862/1‑150 (MQ=60)

                                                                                   ATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAA                                                               >  SRR19973081.1843505/1‑150 (MQ=60)

                                                                                   ATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAA                                                               >  SRR19973081.1983461/1‑150 (MQ=60)

                                                                                    TAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAAC                                                              <  SRR19973081.3263158/150‑1 (MQ=60)

                                                                                          CAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAAT                                                        >  SRR19973081.3456253/1‑150 (MQ=60)

                                                                                          CAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAAT                                                        >  SRR19973081.3457258/1‑150 (MQ=60)

                                                                                           AATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATA                                                       >  SRR19973081.4617212/1‑150 (MQ=60)

                                                                                           AATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATA                                                       <  SRR19973081.3217122/150‑1 (MQ=60)

                                                                                            ATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATAT                                                      >  SRR19973081.2695531/1‑150 (MQ=60)

                                                                                            ATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATAT                                                      <  SRR19973081.2915295/150‑1 (MQ=60)

                                                                                              CTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGA                                                    >  SRR19973081.360667/1‑150 (MQ=60)

                                                                                                  TGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAA                                                >  SRR19973081.3629764/1‑150 (MQ=60)

                                                                                                    TAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATC                                              <  SRR19973081.3885246/150‑1 (MQ=60)

                                                                                                      AAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCAT                                            >  SRR19973081.2251828/1‑150 (MQ=60)

                                                                                                      AAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCAT                                            >  SRR19973081.3657661/1‑150 (MQ=60)

                                                                                                         CTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTGTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGC                                         <  SRR19973081.4419661/150‑1 (MQ=60)

                                                                                                         CTGCAGCGCATTCGTGCGGCTAGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGTCACGCGCTTTCAGTTTCTTTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGC                                         <  SRR19973081.4421253/150‑1 (MQ=60)

                                                                                                          TGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCA                                        <  SRR19973081.2388421/150‑1 (MQ=60)

                                                                                                            CAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACT                                      <  SRR19973081.101584/150‑1 (MQ=60)

                                                                                                            CAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACT                                      <  SRR19973081.3538637/150‑1 (MQ=60)

                                                                                                            CAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACT                                      >  SRR19973081.3378813/1‑150 (MQ=60)

                                                                                                            CAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACT                                      >  SRR19973081.4240211/1‑150 (MQ=60)

                                                                                                             AGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTA                                     >  SRR19973081.1430872/1‑150 (MQ=60)

                                                                                                             AGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTA                                     >  SRR19973081.1886845/1‑150 (MQ=60)

                                                                                                             AGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTA                                     >  SRR19973081.1886879/1‑150 (MQ=60)

                                                                                                               CGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCATGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATT                                   >  SRR19973081.1555279/1‑150 (MQ=60)

                                                                                                               CGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATT                                   <  SRR19973081.2182672/150‑1 (MQ=60)

                                                                                                                  ATTCGTGCGTCTAGTCGATCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGTCACGCTCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACTTGTCATCACCTGGTTAACATTTATATGAAAATTCATAGCACTATTAAT                                <  SRR19973081.3830500/150‑1 (MQ=60)

                                                                                                                    TCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCT                              <  SRR19973081.3280945/150‑1 (MQ=60)

                                                                                                                    TCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCT                              <  SRR19973081.3280887/150‑1 (MQ=60)

                                                                                                                     CGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTA                             <  SRR19973081.4325653/150‑1 (MQ=60)

                                                                                                                     CGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTA                             >  SRR19973081.2507151/1‑150 (MQ=60)

                                                                                                                      GTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTAC                            >  SRR19973081.718442/1‑150 (MQ=60)

                                                                                                                       TGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACT                           >  SRR19973081.2376305/1‑150 (MQ=60)

                                                                                                                       TGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACT                           >  SRR19973081.2376090/1‑150 (MQ=60)

                                                                                                                            CTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGG                      >  SRR19973081.2613258/1‑150 (MQ=60)

                                                                                                                                 CGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGT                 >  SRR19973081.219795/1‑150 (MQ=60)

                                                                                                                                 CGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGT                 >  SRR19973081.219329/1‑150 (MQ=60)

                                                                                                                                  GAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTA                >  SRR19973081.2451701/1‑150 (MQ=60)

                                                                                                                                  GAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTA                <  SRR19973081.1814052/150‑1 (MQ=60)

                                                                                                                                     CATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCA             <  SRR19973081.1369853/150‑1 (MQ=60)

                                                                                                                                      ATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAG            >  SRR19973081.1220794/1‑150 (MQ=60)

                                                                                                                                            TTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTG      <  SRR19973081.878828/150‑1 (MQ=60)

                                                                                                                                                GCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGG  <  SRR19973081.4321288/150‑1 (MQ=60)

TTAGCTCATCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGG  >  CP009273/2179059‑2179352

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`