Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F159 I0 R1 879 305.9 9588012 96.6% 9262019 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,179,205 G→T 100% A24E (GCG→GAG)  rcnR ← transcriptional repressor of rcnA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,179,2050GT96.6% 598.0 / 10.4 175A24E (GCG→GAG) rcnRtranscriptional repressor of rcnA
Reads supporting (aligned to +/- strand):  ref base G (5/1);  new base T (72/97);  total (77/98)
Fisher's exact test for biased strand distribution p-value = 8.82e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 175 total aligned reads displayed.

TTAGCTCATCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGG  >  CP009273/2179059‑2179351
                                                                                                                                                  |                                                                                                                                                  
ttAGCTCATCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCa                                                                                                                                                 <  2:4370122/150‑1 (MQ=255)
ttAGCTCATCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCa                                                                                                                                                 <  2:4370026/150‑1 (MQ=255)
ttAGCTCATCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATGTTCTTGAGCTCCa                                                                                                                                                 >  2:415066/1‑150 (MQ=255)
 tAGCTCATCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCAc                                                                                                                                                <  1:1346915/150‑1 (MQ=255)
   gCTCATCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGa                                                                                                                                              >  2:2653847/1‑150 (MQ=255)
     tCATCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGAcc                                                                                                                                            <  2:3437031/150‑1 (MQ=255)
        tCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTgg                                                                                                                                         >  2:639947/1‑150 (MQ=255)
        tCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTgg                                                                                                                                         <  1:1252012/150‑1 (MQ=255)
            ccTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCt                                                                                                                                     <  1:3690016/150‑1 (MQ=255)
            ccTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCt                                                                                                                                     <  2:2554579/150‑1 (MQ=255)
               ggTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGaa                                                                                                                                  >  1:2793084/1‑150 (MQ=255)
                gTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAAt                                                                                                                                 <  1:1045370/150‑1 (MQ=255)
                   aaCGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCtt                                                                                                                              <  1:1516231/150‑1 (MQ=255)
                    aCGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTa                                                                                                                             >  1:3723265/1‑150 (MQ=255)
                     cGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTAc                                                                                                                            >  1:1124240/1‑150 (MQ=255)
                      gATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACt                                                                                                                           >  1:3755802/1‑150 (MQ=255)
                      gATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACt                                                                                                                           <  2:2329013/150‑1 (MQ=255)
                       aTGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTg                                                                                                                          >  1:3851171/1‑150 (MQ=255)
                           gtTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCAc                                                                                                                      <  2:3141027/150‑1 (MQ=255)
                            ttCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCAcg                                                                                                                     >  1:1002317/1‑150 (MQ=255)
                              ccGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCAcgcg                                                                                                                   >  1:2596618/1‑150 (MQ=255)
                              ccGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCAcgcg                                                                                                                   >  1:4770031/1‑150 (MQ=255)
                              ccGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCAcgcg                                                                                                                   >  1:4767207/1‑150 (MQ=255)
                                   aGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTc                                                                                                              >  2:2219283/1‑150 (MQ=255)
                                     aTGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGATCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAg                                                                                                            >  2:1081202/1‑150 (MQ=255)
                                          cTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCt                                                                                                       <  1:1918606/150‑1 (MQ=255)
                                            ttAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGt                                                                                                     <  1:4171840/150‑1 (MQ=255)
                                             tAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGtt                                                                                                    <  2:215830/150‑1 (MQ=255)
                                               aTCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTa                                                                                                  <  1:879035/150‑1 (MQ=255)
                                               aTCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTa                                                                                                  >  1:722683/1‑150 (MQ=255)
                                               aTCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTa                                                                                                  >  1:3111268/1‑150 (MQ=255)
                                                tCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTAt                                                                                                 >  1:1862946/1‑150 (MQ=255)
                                                 cACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATc                                                                                                <  1:594416/150‑1 (MQ=255)
                                                  aCTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCa                                                                                               <  2:1920656/150‑1 (MQ=255)
                                                   cTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCAc                                                                                              <  1:1702322/150‑1 (MQ=255)
                                                       ccGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGAt                                                                                          <  2:2464828/150‑1 (MQ=255)
                                                        cGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGAtt                                                                                         <  1:2185538/150‑1 (MQ=255)
                                                         gCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTg                                                                                        >  1:2001446/1‑150 (MQ=255)
                                                          cATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGt                                                                                       <  2:3904185/150‑1 (MQ=255)
                                                          cATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGt                                                                                       >  2:3308811/1‑150 (MQ=255)
                                                           aTCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTa                                                                                      <  1:3967707/150‑1 (MQ=255)
                                                            tCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTAt                                                                                     <  1:1103169/150‑1 (MQ=255)
                                                             cAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATg                                                                                    >  2:4122037/1‑150 (MQ=255)
                                                              aGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATga                                                                                   >  1:3834523/1‑150 (MQ=255)
                                                               gACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATgag                                                                                  <  1:182712/150‑1 (MQ=255)
                                                                  cGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACa                                                                               <  2:1001814/150‑1 (MQ=255)
                                                                         cgcgCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAac                                                                        >  1:2031934/1‑150 (MQ=255)
                                                                          gcgcCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAaca                                                                       <  2:1794161/150‑1 (MQ=255)
                                                                           cgcCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAacac                                                                      <  1:1843399/150‑1 (MQ=255)
                                                                            gcCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACAcc                                                                     <  1:4079110/150‑1 (MQ=255)
                                                                              cACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGTCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTg                                                                   <  1:4507780/150‑1 (MQ=255)
                                                                                  gATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTa                                                               <  2:3182446/150‑1 (MQ=255)
                                                                                   aTAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTaa                                                              <  2:4075977/150‑1 (MQ=255)
                                                                                      gcagcaATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACaa                                                           >  2:2912789/1‑150 (MQ=255)
                                                                                        agcaATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGa                                                         <  1:2976353/150‑1 (MQ=255)
                                                                                        agcaATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGa                                                         <  2:4749176/150‑1 (MQ=255)
                                                                                          caATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAat                                                       <  2:608202/150‑1 (MQ=255)
                                                                                          caATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAat                                                       <  2:733555/150‑1 (MQ=255)
                                                                                              cTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGa                                                   >  2:3018145/1‑150 (MQ=255)
                                                                                                  tgtAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGATTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGaaaaa                                               >  2:3798027/1‑150 (MQ=255)
                                                                                                    tAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATc                                             <  1:2267710/150‑1 (MQ=255)
                                                                                                     aaaaCTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCa                                            >  1:3570776/1‑150 (MQ=255)
                                                                                                        aCTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAg                                         <  2:409691/150‑1 (MQ=255)
                                                                                                          tGCAGCGCATTCGTGCGGCTCGTCGAGCTTTTTCTTGAGCTCCACGACCTGGCACTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCa                                       <  2:3588103/150‑1 (MQ=255)
                                                                                                           gCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCAc                                      >  2:158882/1‑150 (MQ=255)
                                                                                                            cAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACt                                     >  1:2035255/1‑150 (MQ=255)
                                                                                                             aGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTa                                    >  1:4251989/1‑150 (MQ=255)
                                                                                                             aGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTa                                    >  2:2220017/1‑150 (MQ=255)
                                                                                                              gcgcATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTAt                                   <  1:2219283/150‑1 (MQ=255)
                                                                                                               cgcATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTAtt                                  <  2:783954/150‑1 (MQ=255)
                                                                                                               cgcATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTAtt                                  >  1:3441575/1‑150 (MQ=255)
                                                                                                               cgcATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTAtt                                  >  1:3441417/1‑150 (MQ=255)
                                                                                                                gcATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTa                                 <  1:2953790/150‑1 (MQ=255)
                                                                                                                 cATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTaa                                >  1:2146009/1‑150 (MQ=255)
                                                                                                                  aTTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAAt                               <  1:3082885/150‑1 (MQ=255)
                                                                                                                   ttCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATc                              <  1:3046851/150‑1 (MQ=255)
                                                                                                                    tCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCt                             <  1:3068312/150‑1 (MQ=255)
                                                                                                                     cGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTa                            <  2:3014042/150‑1 (MQ=255)
                                                                                                                     cGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTa                            <  2:3398973/150‑1 (MQ=255)
                                                                                                                      gTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTAc                           >  2:2251688/1‑150 (MQ=255)
                                                                                                                        gCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTg                         <  2:3682032/150‑1 (MQ=255)
                                                                                                                          ggCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTCTTAATCTACgggg                       >  2:4459383/1‑150 (MQ=255)
                                                                                                                           gCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTgggg                      <  1:3389568/150‑1 (MQ=255)
                                                                                                                           gCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTgggg                      <  1:3389894/150‑1 (MQ=255)
                                                                                                                               gtcgAGAATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTAGTGGGGGgta                  >  1:3363361/1‑150 (MQ=255)
                                                                                                                                  gAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGgtagta               <  2:2690873/150‑1 (MQ=255)
                                                                                                                                   aGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTAt              <  2:4350328/150‑1 (MQ=255)
                                                                                                                                    gCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATc             <  1:2653847/150‑1 (MQ=255)
                                                                                                                                    gCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATc             >  1:3094162/1‑150 (MQ=255)
                                                                                                                                      aTTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAg           >  1:3878497/1‑150 (MQ=255)
                                                                                                                                        tttCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGt         >  2:1611652/1‑150 (MQ=255)
                                                                                                                                          tCTTGAGCTCCACGACCTGGCCCTGAATCGTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTAc       >  2:4271803/1‑150 (MQ=255)
                                                                                                                                           cTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACt      <  2:3474800/150‑1 (MQ=255)
                                                                                                                                             tGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTgg    <  2:310343/150‑1 (MQ=255)
                                                                                                                                              gAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTggg   <  1:4409227/150‑1 (MQ=255)
                                                                                                                                              gAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTggg   >  1:2124409/1‑150 (MQ=255)
                                                                                                                                              gAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTggg   <  2:3909678/150‑1 (MQ=255)
                                                                                                                                               aGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTgggg  >  1:660283/1‑150 (MQ=255)
                                                                                                                                               aGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTgggg  >  2:2012826/1‑150 (MQ=255)
                                                                                                                                               aGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTgggg  >  2:2012551/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
TTAGCTCATCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGG  >  CP009273/2179059‑2179351

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTTTTAGCTCATCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGG  >  CP009273/2179056‑2179351
                                                                                                                                                     |                                                                                                                                                  
GTTTTAGCTCATCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCT                                                                                                                                                    >  SRR19973078.4099208/1‑150 (MQ=60)
 TTTTAGCTCATCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTC                                                                                                                                                   <  SRR19973078.3371803/150‑1 (MQ=60)
 TTTTAGCTCATCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTC                                                                                                                                                   <  SRR19973078.2935527/150‑1 (MQ=60)
 TTTTAGCTCATCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTC                                                                                                                                                   <  SRR19973078.529722/150‑1 (MQ=60)
    TAGCTCATCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCAC                                                                                                                                                <  SRR19973078.1355337/150‑1 (MQ=60)
     AGCTCATCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACG                                                                                                                                               >  SRR19973078.619136/1‑150 (MQ=60)
        TCATCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACC                                                                                                                                            >  SRR19973078.595152/1‑150 (MQ=60)
           TCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGG                                                                                                                                         <  SRR19973078.1259761/150‑1 (MQ=60)
             CCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCC                                                                                                                                       >  SRR19973078.3494597/1‑150 (MQ=60)
               CCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCT                                                                                                                                     <  SRR19973078.3711123/150‑1 (MQ=60)
                 TGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGA                                                                                                                                   >  SRR19973078.4488168/1‑150 (MQ=60)
                  GGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAA                                                                                                                                  >  SRR19973078.2808614/1‑150 (MQ=60)
                  GGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAA                                                                                                                                  >  SRR19973078.659443/1‑150 (MQ=60)
                   GTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAAT                                                                                                                                 <  SRR19973078.1051838/150‑1 (MQ=60)
                    TGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATC                                                                                                                                >  SRR19973078.3762627/1‑150 (MQ=60)
                      AACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTT                                                                                                                              <  SRR19973078.1525395/150‑1 (MQ=60)
                       ACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTA                                                                                                                             >  SRR19973078.3744615/1‑150 (MQ=60)
                        CGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTAC                                                                                                                            >  SRR19973078.1131037/1‑150 (MQ=60)
                         GATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACT                                                                                                                           >  SRR19973078.3777367/1‑150 (MQ=60)
                          ATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTG                                                                                                                          >  SRR19973078.3873202/1‑150 (MQ=60)
                             TGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCA                                                                                                                       <  SRR19973078.4403555/150‑1 (MQ=60)
                               TTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACG                                                                                                                     >  SRR19973078.1008576/1‑150 (MQ=60)
                                 CCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCG                                                                                                                   >  SRR19973078.2611474/1‑150 (MQ=60)
                                 CCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCG                                                                                                                   >  SRR19973078.4793945/1‑150 (MQ=60)
                                 CCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCG                                                                                                                   >  SRR19973078.4796773/1‑150 (MQ=60)
                                    TCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTT                                                                                                                <  SRR19973078.1175930/150‑1 (MQ=60)
                                     CAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTT                                                                                                               >  SRR19973078.4328497/1‑150 (MQ=60)
                                         TGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGT                                                                                                           <  SRR19973078.2599629/150‑1 (MQ=60)
                                          GACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTT                                                                                                          >  SRR19973078.3703077/1‑150 (MQ=60)
                                            CCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTC                                                                                                        >  SRR19973078.1872408/1‑150 (MQ=60)
                                             CTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCT                                                                                                       >  SRR19973078.4056093/1‑150 (MQ=60)
                                             CTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCT                                                                                                       <  SRR19973078.1929919/150‑1 (MQ=60)
                                             CTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCT                                                                                                       <  SRR19973078.1929871/150‑1 (MQ=60)
                                               TTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGT                                                                                                     <  SRR19973078.4195655/150‑1 (MQ=60)
                                               TTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGT                                                                                                     <  SRR19973078.4195545/150‑1 (MQ=60)
                                                TAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTT                                                                                                    <  SRR19973078.2856695/150‑1 (MQ=60)
                                                 AATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTT                                                                                                   >  SRR19973078.2687537/1‑150 (MQ=60)
                                                  ATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTA                                                                                                  >  SRR19973078.3128768/1‑150 (MQ=60)
                                                  ATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTA                                                                                                  <  SRR19973078.884450/150‑1 (MQ=60)
                                                  ATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTA                                                                                                  >  SRR19973078.727085/1‑150 (MQ=60)
                                                   TCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTAT                                                                                                 >  SRR19973078.1874082/1‑150 (MQ=60)
                                                    CACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATC                                                                                                >  SRR19973078.1453289/1‑150 (MQ=60)
                                                    CACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATC                                                                                                <  SRR19973078.598322/150‑1 (MQ=60)
                                                     ACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCA                                                                                               <  SRR19973078.3810865/150‑1 (MQ=60)
                                                      CTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCAC                                                                                              <  SRR19973078.1712687/150‑1 (MQ=60)
                                                           CGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATT                                                                                         <  SRR19973078.2198327/150‑1 (MQ=60)
                                                            GCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTG                                                                                        >  SRR19973078.2013058/1‑150 (MQ=60)
                                                            GCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTG                                                                                        >  SRR19973078.2012531/1‑150 (MQ=60)
                                                            GCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTG                                                                                        >  SRR19973078.3213752/1‑150 (MQ=60)
                                                              ATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTA                                                                                      <  SRR19973078.3990120/150‑1 (MQ=60)
                                                               TCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTAT                                                                                     <  SRR19973078.1109891/150‑1 (MQ=60)
                                                               TCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTAT                                                                                     <  SRR19973078.1109879/150‑1 (MQ=60)
                                                               TCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTAT                                                                                     <  SRR19973078.1109849/150‑1 (MQ=60)
                                                                 AGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGA                                                                                   >  SRR19973078.3858108/1‑150 (MQ=60)
                                                                 AGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGA                                                                                   >  SRR19973078.3856492/1‑150 (MQ=60)
                                                                  GACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAG                                                                                  <  SRR19973078.184239/150‑1 (MQ=60)
                                                                   ACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGA                                                                                 >  SRR19973078.1837494/1‑150 (MQ=60)
                                                                     CGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACA                                                                               <  SRR19973078.95672/150‑1 (MQ=60)
                                                                            CGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAAC                                                                        >  SRR19973078.2043829/1‑150 (MQ=60)
                                                                            CGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAAC                                                                        >  SRR19973078.2043859/1‑150 (MQ=60)
                                                                             GCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACA                                                                       <  SRR19973078.3406848/150‑1 (MQ=60)
                                                                              CGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACAC                                                                      <  SRR19973078.1854455/150‑1 (MQ=60)
                                                                               GCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACC                                                                     <  SRR19973078.4102358/150‑1 (MQ=60)
                                                                                 CACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGTCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTG                                                                   <  SRR19973078.4533290/150‑1 (MQ=60)
                                                                                 CACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTG                                                                   <  SRR19973078.4532563/150‑1 (MQ=60)
                                                                                    GGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTT                                                                >  SRR19973078.676610/1‑150 (MQ=60)
                                                                                           AGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGA                                                         <  SRR19973078.2993333/150‑1 (MQ=60)
                                                                                            GCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAA                                                        >  SRR19973078.222750/1‑150 (MQ=60)
                                                                                              AATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATA                                                      <  SRR19973078.268277/150‑1 (MQ=60)
                                                                                                       TAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATC                                             <  SRR19973078.2280808/150‑1 (MQ=60)
                                                                                                        AAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCA                                            >  SRR19973078.3590872/1‑150 (MQ=60)
                                                                                                               CAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACT                                     >  SRR19973078.2047188/1‑150 (MQ=60)
                                                                                                                AGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGTATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTA                                    >  SRR19973078.2982724/1‑150 (MQ=60)
                                                                                                                AGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTA                                    >  SRR19973078.4276176/1‑150 (MQ=60)
                                                                                                                 GCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTAT                                   <  SRR19973078.2232222/150‑1 (MQ=60)
                                                                                                                 GCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTAT                                   <  SRR19973078.2232541/150‑1 (MQ=60)
                                                                                                                  CGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATT                                  >  SRR19973078.156941/1‑150 (MQ=60)
                                                                                                                  CGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATT                                  >  SRR19973078.3461076/1‑150 (MQ=60)
                                                                                                                  CGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATT                                  >  SRR19973078.3461197/1‑150 (MQ=60)
                                                                                                                  CGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATT                                  >  SRR19973078.3461235/1‑150 (MQ=60)
                                                                                                                   GCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTA                                 <  SRR19973078.2970652/150‑1 (MQ=60)
                                                                                                                   GCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTA                                 >  SRR19973078.3914060/1‑150 (MQ=60)
                                                                                                                    CATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAA                                >  SRR19973078.2158212/1‑150 (MQ=60)
                                                                                                                    CATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAA                                >  SRR19973078.2158590/1‑150 (MQ=60)
                                                                                                                     ATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAAT                               <  SRR19973078.3098388/150‑1 (MQ=60)
                                                                                                                     ATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAAT                               <  SRR19973078.3100290/150‑1 (MQ=60)
                                                                                                                     ATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAAT                               >  SRR19973078.3307926/1‑150 (MQ=60)
                                                                                                                      TTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATC                              <  SRR19973078.3064122/150‑1 (MQ=60)
                                                                                                                      TTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAACC                              <  SRR19973078.3065170/150‑1 (MQ=60)
                                                                                                                       TCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCT                             <  SRR19973078.3085671/150‑1 (MQ=60)
                                                                                                                            CGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTAatgt                        >  SRR19973078.311263/1‑146 (MQ=60)
                                                                                                                              GCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGG                      <  SRR19973078.3409297/150‑1 (MQ=60)
                                                                                                                              GCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGG                      <  SRR19973078.3408992/150‑1 (MQ=60)
                                                                                                                              GCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGG                      <  SRR19973078.3408967/150‑1 (MQ=60)
                                                                                                                                  GTCGAGAATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTAGTGGGGGGTA                  >  SRR19973078.3382611/1‑150 (MQ=60)
                                                                                                                                       GCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATC             >  SRR19973078.3111593/1‑150 (MQ=60)
                                                                                                                                       GCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATC             <  SRR19973078.2130801/150‑1 (MQ=60)
                                                                                                                                       GCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATC             <  SRR19973078.2668968/150‑1 (MQ=60)
                                                                                                                                        CATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCA            <  SRR19973078.4537165/150‑1 (MQ=60)
                                                                                                                                         ATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAG           >  SRR19973078.3900624/1‑150 (MQ=60)
                                                                                                                                                 GAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGG   >  SRR19973078.2136894/1‑150 (MQ=60)
                                                                                                                                                 GAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGG   <  SRR19973078.4433980/150‑1 (MQ=60)
                                                                                                                                                  AGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGG  >  SRR19973078.664474/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                  
GTTTTAGCTCATCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCAGACCGTTTACCGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGG  >  CP009273/2179056‑2179351

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑