Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F86 I0 R1 593 297.4 9515568 94.4% 8982696 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,450,335 C→T 93.2% intergenic (‑44/+159) sixA ← / ← fadJ phosphohistidine phosphatase/fused enoyl‑CoA hydratase and epimerase and isomerase/3‑hydroxyacyl‑CoA dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,450,3350CT93.2% 835.0 / 30.3 248intergenic (‑44/+159)sixA/fadJphosphohistidine phosphatase/fused enoyl‑CoA hydratase and epimerase and isomerase/3‑hydroxyacyl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (4/13);  new base T (84/147);  total (88/160)
Fisher's exact test for biased strand distribution p-value = 4.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 248 total aligned reads displayed.

GCCATCAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATA  >  CP009273/2450188‑2450481
                                                                                                                                                   |                                                                                                                                                  
gCCATCAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTg                                                                                                                                                  <  1:1916933/150‑1 (MQ=255)
 ccATCAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGa                                                                                                                                                 <  2:2525919/150‑1 (MQ=255)
     cAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAAcc                                                                                                                                             >  2:4641823/1‑150 (MQ=255)
     cAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAAcc                                                                                                                                             >  2:2200759/1‑150 (MQ=255)
       ggCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCag                                                                                                                                           >  1:3370461/1‑150 (MQ=255)
       ggCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCag                                                                                                                                           <  1:493698/150‑1 (MQ=255)
       ggCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCag                                                                                                                                           <  1:3817305/150‑1 (MQ=255)
        gCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCaga                                                                                                                                          <  1:3800334/150‑1 (MQ=255)
           agaTTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGataa                                                                                                                                       <  1:606979/150‑1 (MQ=255)
            gaTTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGataaa                                                                                                                                      <  2:2451163/150‑1 (MQ=255)
             aTTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGataaag                                                                                                                                     <  1:1436216/150‑1 (MQ=255)
              ttCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGataaaga                                                                                                                                    <  2:1842484/150‑1 (MQ=255)
                  tCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAAc                                                                                                                                <  2:3966363/150‑1 (MQ=255)
                    acaACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCATTTTCTTTCATTTGAAACCAGATAAAGAAAACgg                                                                                                                              <  2:2592630/150‑1 (MQ=255)
                     caACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGt                                                                                                                             <  2:1610568/150‑1 (MQ=255)
                      aaCCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTg                                                                                                                            <  2:3254461/150‑1 (MQ=255)
                        ccATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTTTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGc                                                                                                                          <  2:492175/150‑1 (MQ=255)
                               tGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCAtt                                                                                                                   <  1:4497178/150‑1 (MQ=255)
                               tGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCAtt                                                                                                                   >  2:2967034/1‑150 (MQ=255)
                               tGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCAtt                                                                                                                   <  1:2114388/150‑1 (MQ=255)
                                 gTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATtgt                                                                                                                 <  2:3264348/150‑1 (MQ=255)
                                   cAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGc                                                                                                               >  1:835277/1‑150 (MQ=255)
                                   cAGAGGACGAACGGAATCACTGACGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGc                                                                                                               <  1:472915/150‑1 (MQ=255)
                                    agagGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGcc                                                                                                              >  2:1371414/1‑150 (MQ=255)
                                      agGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCtt                                                                                                            >  2:3958758/1‑150 (MQ=255)
                                         aCGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATc                                                                                                         <  1:1002728/150‑1 (MQ=255)
                                         aCGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCACTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATc                                                                                                         <  2:2660160/150‑1 (MQ=255)
                                          cGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATcc                                                                                                        <  2:2148068/150‑1 (MQ=255)
                                            accGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCTATTGAAACCAGATAAAGAAAACGGTGGCCGGCAGGGTGCCTTATCCAt                                                                                                      <  1:2060176/148‑1 (MQ=255)
                                              cGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTc                                                                                                    >  2:1922114/1‑150 (MQ=255)
                                              cGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTc                                                                                                    >  2:349649/1‑150 (MQ=255)
                                               ggAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCa                                                                                                   <  1:263297/150‑1 (MQ=255)
                                                 aaTCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCAcc                                                                                                 <  1:1390452/150‑1 (MQ=255)
                                                 aaTCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCAcc                                                                                                 <  2:867027/150‑1 (MQ=255)
                                                   tCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGa                                                                                               <  2:762119/150‑1 (MQ=255)
                                                      cTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATg                                                                                            <  2:3371286/150‑1 (MQ=255)
                                                      cTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATg                                                                                            >  1:1058552/1‑150 (MQ=255)
                                                       tGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGa                                                                                           <  2:556075/150‑1 (MQ=255)
                                                              aTCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTg                                                                                    <  2:1742786/150‑1 (MQ=255)
                                                                 gAGGGCTGCGTCGCCGTGACGCGTGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGttt                                                                                 <  2:1231825/150‑1 (MQ=255)
                                                                 gAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGttt                                                                                 >  1:3808302/1‑150 (MQ=255)
                                                                  aGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGtttt                                                                                >  2:530327/1‑150 (MQ=255)
                                                                     gCTGCGTCGCCGTGACGCTTGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACAAGATAAAGAAAACGGTGGCCGGCTTTGTGCCTTATCCATTCACCGAATGAATCGCTGTTTTTTa                                                                             <  2:2306841/150‑1 (MQ=255)
                                                                     gCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTa                                                                             <  1:2909906/150‑1 (MQ=255)
                                                                      cTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTAc                                                                            <  2:1702579/150‑1 (MQ=255)
                                                                          gTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCa                                                                        <  1:4196580/150‑1 (MQ=255)
                                                                                gTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCg                                                                  <  1:1636827/150‑1 (MQ=255)
                                                                                gTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCAGTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCg                                                                  <  1:1282124/150‑1 (MQ=255)
                                                                                  gACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTa                                                                >  2:4284232/1‑150 (MQ=255)
                                                                                   aCGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTaa                                                               >  2:3792640/1‑150 (MQ=255)
                                                                                      cATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGta                                                            <  2:4509801/150‑1 (MQ=255)
                                                                                       aTGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGtat                                                           <  2:2174639/150‑1 (MQ=255)
                                                                                        tGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGtata                                                          >  2:2765916/1‑150 (MQ=255)
                                                                                         gATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAg                                                         <  2:3777219/150‑1 (MQ=255)
                                                                                         gATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAg                                                         <  1:2377135/150‑1 (MQ=255)
                                                                                          aTAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGt                                                        >  1:3033365/1‑150 (MQ=255)
                                                                                           tAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTc                                                       <  2:3177853/150‑1 (MQ=255)
                                                                                            aaaaaCTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCa                                                      >  1:1751347/1‑150 (MQ=255)
                                                                                            aaaaaCTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCa                                                      <  2:408190/150‑1 (MQ=255)
                                                                                              taaCTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAat                                                    >  2:1761050/2‑150 (MQ=255)
                                                                                              aaaCTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAat                                                    >  2:714267/1‑150 (MQ=255)
                                                                                               aaCTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTATTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCGTATACATTAACCGAAAGAAACGCGGTTTTTTAACTCAATGGCGTAAGTATATTCAata                                                   >  1:4234723/1‑150 (MQ=255)
                                                                                                aCTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAatat                                                  <  2:4438589/150‑1 (MQ=255)
                                                                                                  ttGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGc                                                <  2:4336498/150‑1 (MQ=255)
                                                                                                  ttGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGc                                                <  2:1955029/150‑1 (MQ=255)
                                                                                                       tatTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTa                                           <  2:1091362/150‑1 (MQ=255)
                                                                                                       tatTGCACCGCTTTTGTTAACCAGAGTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTa                                           <  2:3389347/150‑1 (MQ=255)
                                                                                                        atTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTAc                                          >  1:4523736/1‑150 (MQ=255)
                                                                                                        atTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTAc                                          <  1:729576/150‑1 (MQ=255)
                                                                                                          tGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATTGCGTAAGTATAGTCAATATGCGTTTACAt                                        >  1:4027340/1‑150 (MQ=255)
                                                                                                          tGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACAt                                        <  2:4587085/150‑1 (MQ=255)
                                                                                                            cACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACAttt                                      <  2:3668601/150‑1 (MQ=255)
                                                                                                             aCCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCAGTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACAtttt                                     <  2:528924/150‑1 (MQ=255)
                                                                                                              ccGCTTTTGTTAACCAGATGCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTg                                    <  2:4491809/150‑1 (MQ=255)
                                                                                                                  ttttGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCa                                >  1:3109787/1‑150 (MQ=255)
                                                                                                                   tttGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCaa                               <  1:2408703/150‑1 (MQ=255)
                                                                                                                    ttGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAATTATAGTCAATATGCGTTTACATTTTGACCAac                              >  1:1180790/1‑150 (MQ=255)
                                                                                                                      gTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAacac                            >  2:3342514/1‑150 (MQ=255)
                                                                                                                        tAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTc                          <  2:267732/150‑1 (MQ=255)
                                                                                                                         aaCCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTcc                         <  2:2863140/150‑1 (MQ=255)
                                                                                                                          aCCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCg                        <  1:3788940/150‑1 (MQ=255)
                                                                                                                             aGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCa                     <  1:2003184/150‑1 (MQ=255)
                                                                                                                             aGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCa                     <  2:1305175/150‑1 (MQ=255)
                                                                                                                             aGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCa                     >  2:4227602/1‑150 (MQ=255)
                                                                                                                               aTTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCAtt                   <  1:4084311/150‑1 (MQ=255)
                                                                                                                                  cGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAg                <  1:1224535/150‑1 (MQ=255)
                                                                                                                                  cGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAg                <  1:3620624/150‑1 (MQ=255)
                                                                                                                                    cccGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAgcg              <  1:2175896/150‑1 (MQ=255)
                                                                                                                                      cGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGcgcg            <  1:1159583/150‑1 (MQ=255)
                                                                                                                                        ttttCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGa          >  1:3564685/1‑150 (MQ=255)
                                                                                                                                         tttctttcATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGAt         >  1:1901406/1‑150 (MQ=255)
                                                                                                                                          ttctttcATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGAtt        >  1:1972753/1‑150 (MQ=255)
                                                                                                                                          ttctttcATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGAtt        <  2:1825775/150‑1 (MQ=255)
                                                                                                                                           tctttcATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTc       >  1:98188/1‑150 (MQ=255)
                                                                                                                                            ctttcATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCa      <  2:4065717/150‑1 (MQ=255)
                                                                                                                                              ttcATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCata    <  1:862070/150‑1 (MQ=255)
                                                                                                                                               tcATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCatat   >  1:1753997/1‑150 (MQ=255)
                                                                                                                                               tcATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCatat   <  2:3195187/150‑1 (MQ=255)
                                                                                                                                               tcATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCatat   <  1:3467452/150‑1 (MQ=255)
                                                                                                                                                cATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCAtata  >  2:4173235/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GCCATCAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATA  >  CP009273/2450188‑2450481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCGCCATCAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGC  >  CP009273/2450186‑2450483
                                                                                                                                                     |                                                                                                                                                    
TCGCCATCAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATT                                                                                                                                                      <  SRR19973082.262011/150‑1 (MQ=60)
TCGCCATCAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATT                                                                                                                                                      <  SRR19973082.259817/150‑1 (MQ=60)
TCGCCATCAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATT                                                                                                                                                      <  SRR19973082.259726/150‑1 (MQ=60)
TCGCCATCAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATT                                                                                                                                                      >  SRR19973082.1713898/1‑150 (MQ=60)
 CGCCATCAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTT                                                                                                                                                     <  SRR19973082.2715223/150‑1 (MQ=60)
 CGCCATCAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGAGTCGCCCGTTTTCTTTCATTT                                                                                                                                                     <  SRR19973082.2379612/150‑1 (MQ=60)
  GCCATCAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCTCTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTG                                                                                                                                                    <  SRR19973082.657/150‑1 (MQ=60)
  GCCATCAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTG                                                                                                                                                    <  SRR19973082.982065/150‑1 (MQ=60)
  GCCATCAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTG                                                                                                                                                    <  SRR19973082.1929142/150‑1 (MQ=60)
  GCCATCAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTG                                                                                                                                                    <  SRR19973082.1928770/150‑1 (MQ=60)
  GCCATCAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTG                                                                                                                                                    >  SRR19973082.4465513/1‑150 (MQ=60)
    CATCAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAA                                                                                                                                                  >  SRR19973082.2322454/1‑150 (MQ=60)
      TCAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAAC                                                                                                                                                <  SRR19973082.3711384/150‑1 (MQ=60)
      TCAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAAC                                                                                                                                                <  SRR19973082.780050/150‑1 (MQ=60)
      TCAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATAGTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAAC                                                                                                                                                <  SRR19973082.779978/150‑1 (MQ=60)
         GGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATACAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAG                                                                                                                                             >  SRR19973082.1310164/1‑150 (MQ=60)
         GGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAG                                                                                                                                             >  SRR19973082.3391304/1‑150 (MQ=60)
         GGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAG                                                                                                                                             <  SRR19973082.3840841/150‑1 (MQ=60)
         GGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAG                                                                                                                                             <  SRR19973082.3841414/150‑1 (MQ=60)
         GGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAG                                                                                                                                             <  SRR19973082.497214/150‑1 (MQ=60)
          GCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGA                                                                                                                                            <  SRR19973082.3823869/150‑1 (MQ=60)
          GCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGA                                                                                                                                            <  SRR19973082.3823794/150‑1 (MQ=60)
             AGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAA                                                                                                                                         <  SRR19973082.611289/150‑1 (MQ=60)
               ATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAG                                                                                                                                       >  SRR19973082.4532343/1‑150 (MQ=60)
               ATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAG                                                                                                                                       <  SRR19973082.1445987/150‑1 (MQ=60)
                    TCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAAC                                                                                                                                  >  SRR19973082.4613683/1‑150 (MQ=60)
                     CACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACG                                                                                                                                 >  SRR19973082.1443403/1‑150 (MQ=60)
                            ATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCG                                                                                                                          >  SRR19973082.1934471/1‑150 (MQ=60)
                               AGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCA                                                                                                                       >  SRR19973082.588288/1‑150 (MQ=60)
                               AGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCA                                                                                                                       >  SRR19973082.4766927/1‑150 (MQ=60)
                                 TGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATT                                                                                                                     <  SRR19973082.2033702/150‑1 (MQ=60)
                                 TGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATT                                                                                                                     <  SRR19973082.2127848/150‑1 (MQ=60)
                                 TGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATT                                                                                                                     <  SRR19973082.4524520/150‑1 (MQ=60)
                                     CAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGC                                                                                                                 >  SRR19973082.840837/1‑150 (MQ=60)
                                     CAGAGGACGAACGGAATCACTGACGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGC                                                                                                                 <  SRR19973082.3444330/150‑1 (MQ=60)
                                     CAGAGGACGAACGGAATCACTGACGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGC                                                                                                                 <  SRR19973082.476254/150‑1 (MQ=60)
                                           ACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATC                                                                                                           <  SRR19973082.1009712/150‑1 (MQ=60)
                                           ACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATC                                                                                                           <  SRR19973082.2418810/150‑1 (MQ=60)
                                            CGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCC                                                                                                          <  SRR19973082.1758966/150‑1 (MQ=60)
                                            CGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCC                                                                                                          <  SRR19973082.4163976/150‑1 (MQ=60)
                                            CGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCC                                                                                                          <  SRR19973082.4163896/150‑1 (MQ=60)
                                              tcCGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTTACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCTATTGAAACCAGATAAAGAAAACGGTGGCCGGCAGGGTGCCTTATCCAT                                                                                                        <  SRR19973082.2073302/148‑1 (MQ=60)
                                              AACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCAT                                                                                                        <  SRR19973082.2074453/150‑1 (MQ=60)
                                                 GGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCA                                                                                                     >  SRR19973082.3633811/1‑150 (MQ=60)
                                                 GGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCA                                                                                                     <  SRR19973082.265282/150‑1 (MQ=60)
                                                 GGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCA                                                                                                     <  SRR19973082.3362071/150‑1 (MQ=60)
                                                  GAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCAC                                                                                                    >  SRR19973082.4090644/1‑150 (MQ=60)
                                                   AATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACC                                                                                                   <  SRR19973082.3806203/150‑1 (MQ=60)
                                                   AATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACC                                                                                                   <  SRR19973082.1399998/150‑1 (MQ=60)
                                                        CTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAATCTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAACACGGTGGCCGGCCTTGTGCCTTATCCAGTCACCGACTG                                                                                              >  SRR19973082.3466755/1‑150 (MQ=60)
                                                        CTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATG                                                                                              >  SRR19973082.1065831/1‑150 (MQ=60)
                                                        CTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATG                                                                                              >  SRR19973082.1065813/1‑150 (MQ=60)
                                                         TGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGA                                                                                             >  SRR19973082.861217/1‑150 (MQ=60)
                                                          GGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAA                                                                                            <  SRR19973082.1261866/150‑1 (MQ=60)
                                                             GGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACG                                                                                         <  SRR19973082.2989747/150‑1 (MQ=60)
                                                                   GAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTT                                                                                   >  SRR19973082.3831793/1‑150 (MQ=60)
                                                                    AGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTT                                                                                  >  SRR19973082.2168041/1‑150 (MQ=60)
                                                                     GGGCTGCGTCGCCGTGACGCATGATAAAAACCTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTT                                                                                 <  SRR19973082.3072449/150‑1 (MQ=60)
                                                                       GCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTA                                                                               <  SRR19973082.2927887/150‑1 (MQ=60)
                                                                           CGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTC                                                                           <  SRR19973082.1254560/150‑1 (MQ=60)
                                                                            GTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCA                                                                          <  SRR19973082.4222200/150‑1 (MQ=60)
                                                                                CCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGG                                                                      <  SRR19973082.3988971/150‑1 (MQ=60)
                                                                                  GTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCG                                                                    <  SRR19973082.1647703/150‑1 (MQ=60)
                                                                                  GTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCAGTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCG                                                                    <  SRR19973082.1290970/150‑1 (MQ=60)
                                                                                       GCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGT                                                               <  SRR19973082.1429614/150‑1 (MQ=60)
                                                                                           GATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAG                                                           <  SRR19973082.2392560/150‑1 (MQ=60)
                                                                                           GATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAG                                                           >  SRR19973082.1177642/1‑150 (MQ=60)
                                                                                           GATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAG                                                           <  SRR19973082.2391713/150‑1 (MQ=60)
                                                                                            ATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGT                                                          >  SRR19973082.3052024/1‑150 (MQ=60)
                                                                                              AAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCA                                                        >  SRR19973082.1762958/1‑150 (MQ=60)
                                                                                              AAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCA                                                        <  SRR19973082.3089352/150‑1 (MQ=60)
                                                                                                 AACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTATTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATACATTAACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATA                                                     >  SRR19973082.1861278/1‑150 (MQ=60)
                                                                                                 AACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTATTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCGTATACATTAACCGAAAGAAACGCGGTTTTTTAACTCAATGGCGTAAGTATATTCAATA                                                     >  SRR19973082.4260516/1‑150 (MQ=60)
                                                                                                   CTTGCATATTGCACCGCTTTTGTTAACCAGAGTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATG                                                   <  SRR19973082.1549108/150‑1 (MQ=60)
                                                                                                     TGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCG                                                 >  SRR19973082.245474/1‑150 (MQ=60)
                                                                                                       CATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTT                                               >  SRR19973082.198289/1‑150 (MQ=60)
                                                                                                        ATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTT                                              <  SRR19973082.1807784/150‑1 (MQ=60)
                                                                                                          ATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTAC                                            <  SRR19973082.734410/150‑1 (MQ=60)
                                                                                                          ATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTAC                                            >  SRR19973082.4551251/1‑150 (MQ=60)
                                                                                                            TGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATTGCGTAAGTATAGTCAATATGCGTTTACAT                                          >  SRR19973082.4052005/1‑150 (MQ=60)
                                                                                                              CACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTT                                        >  SRR19973082.3826431/1‑150 (MQ=60)
                                                                                                              CACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGACAATATGCGTTTACATTT                                        >  SRR19973082.3822583/1‑150 (MQ=60)
                                                                                                                CCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGAGTTTACATTTTG                                      >  SRR19973082.3513354/1‑150 (MQ=60)
                                                                                                                    TTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCA                                  >  SRR19973082.3128709/1‑150 (MQ=60)
                                                                                                                     TTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAA                                 <  SRR19973082.2423631/150‑1 (MQ=60)
                                                                                                                      TTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAATTATAGTCAATATGCGTTTACATTTTGACCAAC                                >  SRR19973082.1188577/1‑150 (MQ=60)
                                                                                                                        GTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACAC                              >  SRR19973082.3839461/1‑150 (MQ=60)
                                                                                                                        GTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACAC                              <  SRR19973082.424110/150‑1 (MQ=60)
                                                                                                                          TAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCCTTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTC                            >  SRR19973082.499037/1‑150 (MQ=60)
                                                                                                                          TAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTC                            >  SRR19973082.4670559/1‑150 (MQ=60)
                                                                                                                           AACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCC                           <  SRR19973082.1144590/150‑1 (MQ=60)
                                                                                                                            ACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCG                          <  SRR19973082.3812333/150‑1 (MQ=60)
                                                                                                                               AGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATATTCAATATGCGTTTACATTTTGACCAACACTCCGCCA                       >  SRR19973082.948015/1‑150 (MQ=60)
                                                                                                                               AGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCA                       <  SRR19973082.4413399/150‑1 (MQ=60)
                                                                                                                               AGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCA                       <  SRR19973082.2015864/150‑1 (MQ=60)
                                                                                                                               AGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCA                       <  SRR19973082.2015770/150‑1 (MQ=60)
                                                                                                                               AGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCA                       <  SRR19973082.2015807/150‑1 (MQ=60)
                                                                                                                                 ATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATT                     <  SRR19973082.4109337/150‑1 (MQ=60)
                                                                                                                                 ATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATT                     <  SRR19973082.1708882/150‑1 (MQ=60)
                                                                                                                                    CGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAG                  <  SRR19973082.3642854/150‑1 (MQ=60)
                                                                                                                                    CGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAG                  <  SRR19973082.1232877/150‑1 (MQ=60)
                                                                                                                                    CGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAG                  <  SRR19973082.1232851/150‑1 (MQ=60)
                                                                                                                                      CCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCG                <  SRR19973082.2189473/150‑1 (MQ=60)
                                                                                                                                      CCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCG                <  SRR19973082.2189686/150‑1 (MQ=60)
                                                                                                                                       CCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGC               <  SRR19973082.2874780/150‑1 (MQ=60)
                                                                                                                                        CGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCG              <  SRR19973082.1167231/150‑1 (MQ=60)
                                                                                                                                        CGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCG              <  SRR19973082.1167201/150‑1 (MQ=60)
                                                                                                                                          TTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGA            >  SRR19973082.3586288/1‑150 (MQ=60)
                                                                                                                                           TTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGAT           >  SRR19973082.1913581/1‑150 (MQ=60)
                                                                                                                                            TTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATT          >  SRR19973082.1985109/1‑150 (MQ=60)
                                                                                                                                             TCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTC         >  SRR19973082.99160/1‑150 (MQ=60)
                                                                                                                                                TTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATA      <  SRR19973082.867929/150‑1 (MQ=60)
                                                                                                                                                 TCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATAT     >  SRR19973082.1764543/1‑150 (MQ=60)
                                                                                                                                                 TCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATAT     >  SRR19973082.1765626/1‑150 (MQ=60)
                                                                                                                                                 TCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATAT     <  SRR19973082.3488746/150‑1 (MQ=60)
                                                                                                                                                 TCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATAT     <  SRR19973082.2893993/150‑1 (MQ=60)
                                                                                                                                                   ATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAG   <  SRR19973082.3982794/150‑1 (MQ=60)
                                                                                                                                                   ATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGAAAGTATAGACAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAG   >  SRR19973082.1779955/1‑150 (MQ=60)
                                                                                                                                                    TTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGC  >  SRR19973082.3333729/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
TCGCCATCAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGC  >  CP009273/2450186‑2450483

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑