Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F121 I0 R1 856 273.6 9081716 94.1% 8545894 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,450,335 C→T 89.9% intergenic (‑44/+159) sixA ← / ← fadJ phosphohistidine phosphatase/fused enoyl‑CoA hydratase and epimerase and isomerase/3‑hydroxyacyl‑CoA dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,450,3350CT89.9% 742.6 / 52.9 238intergenic (‑44/+159)sixA/fadJphosphohistidine phosphatase/fused enoyl‑CoA hydratase and epimerase and isomerase/3‑hydroxyacyl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (4/20);  new base T (94/119);  total (99/139)
Fisher's exact test for biased strand distribution p-value = 9.01e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 239 total aligned reads displayed.

TCGCCATCAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATA  >  CP009273/2450186‑2450481
                                                                                                                                                     |                                                                                                                                                  
tCGCCATCAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATc                                                                                                                                                    >  1:1424423/1‑150 (MQ=255)
   ccATCAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGa                                                                                                                                                 >  1:1574995/1‑150 (MQ=255)
   ccATCAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGa                                                                                                                                                 >  2:2007693/1‑150 (MQ=255)
      tCAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAAc                                                                                                                                              <  1:785833/150‑1 (MQ=255)
       cAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAAcc                                                                                                                                             <  2:908294/150‑1 (MQ=255)
       cAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAAcc                                                                                                                                             >  1:447634/1‑150 (MQ=255)
       cAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAAcc                                                                                                                                             >  2:2629779/1‑150 (MQ=255)
         ggCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCag                                                                                                                                           >  2:191141/1‑150 (MQ=255)
           cGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCagat                                                                                                                                         <  1:1670862/150‑2 (MQ=255)
           cGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCagat                                                                                                                                         <  2:1718626/150‑2 (MQ=255)
           cGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCagat                                                                                                                                         <  2:3466774/150‑2 (MQ=255)
            gagaTTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCagata                                                                                                                                        >  2:3126848/1‑148 (MQ=255)
            gagaTTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCagata                                                                                                                                        >  1:3289550/1‑148 (MQ=255)
             agaTTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGagaa                                                                                                                                       <  2:1071623/150‑1 (MQ=255)
              gaTTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGataaa                                                                                                                                      <  1:308813/150‑1 (MQ=255)
                  cGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGAtaaagaaa                                                                                                                                  <  2:3696466/150‑1 (MQ=255)
                    tCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAAc                                                                                                                                <  1:1041327/150‑1 (MQ=255)
                    tCACAACCATGAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAAc                                                                                                                                <  1:2626137/150‑1 (MQ=255)
                     cacaACCATTTGTGGTCAGAGGACGAACGGAATCACTGGGGGCTTCGAGGGCTGCGTCGGCGTGACGCATGATTAAAACTTGCATATTGCTCAGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACg                                                                                                                               <  2:3843639/150‑1 (MQ=255)
                            aTTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCg                                                                                                                        <  2:613172/150‑1 (MQ=255)
                            aTTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCg                                                                                                                        <  2:2097935/150‑1 (MQ=255)
                            aTTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCg                                                                                                                        <  2:1125106/150‑1 (MQ=255)
                               aGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCa                                                                                                                     >  2:3525474/1‑150 (MQ=255)
                                gTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCAt                                                                                                                    <  1:3444169/150‑1 (MQ=255)
                                 tGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCAtt                                                                                                                   <  2:363750/150‑1 (MQ=255)
                                 tGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGGAAAAGGTGGCCGGCAtt                                                                                                                   >  1:4509274/1‑150 (MQ=255)
                                   gTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATtgt                                                                                                                 <  2:2486247/150‑1 (MQ=255)
                                         ggACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTa                                                                                                           <  1:243850/150‑1 (MQ=255)
                                            cGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATcc                                                                                                        >  2:250798/1‑150 (MQ=255)
                                             gAACGGAATCACTGGCGGCATCGAGGGCTGAGTCTCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAAACAGATTCGCCAGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCa                                                                                                       >  2:2065734/1‑150 (MQ=255)
                                              aaCGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCAt                                                                                                      >  1:1003859/1‑150 (MQ=255)
                                                cGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTc                                                                                                    <  1:4048352/150‑1 (MQ=255)
                                                       aCTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAAt                                                                                             >  1:4090897/1‑150 (MQ=255)
                                                          ggcggcATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGaa                                                                                          <  1:2891393/150‑1 (MQ=255)
                                                            cggcATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATAGTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAAc                                                                                        <  1:1865968/150‑1 (MQ=255)
                                                               cATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCt                                                                                     >  1:2143341/1‑150 (MQ=255)
                                                                aTCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAATAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTg                                                                                    <  1:3871524/150‑1 (MQ=255)
                                                                    aGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGtttt                                                                                <  2:1434768/150‑1 (MQ=255)
                                                                    aGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGtttt                                                                                <  2:529626/150‑1 (MQ=255)
                                                                    aGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATAGTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGtttt                                                                                <  2:4096240/150‑1 (MQ=255)
                                                                      ggCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGtttttt                                                                              <  1:3347943/150‑1 (MQ=255)
                                                                       gCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTa                                                                             <  1:4278990/150‑1 (MQ=255)
                                                                       gCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTa                                                                             <  1:957713/150‑1 (MQ=255)
                                                                          gCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCt                                                                          <  1:3427740/150‑1 (MQ=255)
                                                                           cGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTc                                                                         <  1:3564066/150‑1 (MQ=255)
                                                                           cGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTc                                                                         <  2:2502116/150‑1 (MQ=255)
                                                                              cGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGAGTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAAt                                                                      <  2:3768265/150‑1 (MQ=255)
                                                                                 cGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGc                                                                   >  2:1371501/1‑150 (MQ=255)
                                                                                  gTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCg                                                                  <  2:1003785/150‑1 (MQ=255)
                                                                                  gTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCg                                                                  >  2:1638726/1‑150 (MQ=255)
                                                                                    gACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTa                                                                <  1:722797/150‑1 (MQ=255)
                                                                                     aCGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTaa                                                               >  1:1204746/1‑150 (MQ=255)
                                                                                     aCGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGAGTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTaa                                                               <  2:3184209/150‑1 (MQ=255)
                                                                                      cGCATGATAAAATCTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAg                                                              <  2:3801835/150‑1 (MQ=255)
                                                                                      cGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAg                                                              <  2:337235/150‑1 (MQ=255)
                                                                                      cGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAg                                                              <  2:2281166/150‑1 (MQ=255)
                                                                                       gCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGt                                                             <  2:905133/150‑1 (MQ=255)
                                                                                       gCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGt                                                             <  2:3289624/150‑1 (MQ=255)
                                                                                       gCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGt                                                             >  2:4411641/1‑150 (MQ=255)
                                                                                       gCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGt                                                             >  1:4229675/1‑150 (MQ=255)
                                                                                         aTGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGtat                                                           <  2:2405297/150‑1 (MQ=255)
                                                                                           gATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACATCAATGGCGTAAGTATAg                                                         >  2:4098662/1‑150 (MQ=255)
                                                                                            aTAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGt                                                        >  2:2808645/1‑150 (MQ=255)
                                                                                            aTAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGt                                                        <  1:4410320/150‑1 (MQ=255)
                                                                                             tAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCCATGGCGTAAGTATAGTc                                                       >  2:3254843/1‑150 (MQ=255)
                                                                                             tAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTc                                                       <  1:326169/150‑1 (MQ=255)
                                                                                             tAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTAACTCAATGGCGTAAGTATAGTc                                                       >  1:1543174/1‑150 (MQ=255)
                                                                                              aaaaaCTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCa                                                      >  2:3055020/1‑150 (MQ=255)
                                                                                              aaaaaCTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCa                                                      >  2:3056712/1‑150 (MQ=255)
                                                                                              aaaaaCTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCa                                                      <  2:3072552/150‑1 (MQ=255)
                                                                                                   cTTGCATATTGCACCGCTTGTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCAGTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATg                                                 <  2:2397512/150‑1 (MQ=255)
                                                                                                      gCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGt                                              >  1:3170896/1‑150 (MQ=255)
                                                                                                       cATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGtt                                             >  2:2179096/1‑150 (MQ=255)
                                                                                                        atatTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGttt                                            <  2:4012667/150‑1 (MQ=255)
                                                                                                         tatTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTa                                           <  2:2469003/150‑1 (MQ=255)
                                                                                                         tatTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTa                                           >  1:4523054/1‑150 (MQ=255)
                                                                                                            tGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACAt                                        <  1:2994565/150‑1 (MQ=255)
                                                                                                             gCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACAtt                                       >  1:2915341/1‑150 (MQ=255)
                                                                                                             gCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACAtt                                       >  1:1704903/1‑150 (MQ=255)
                                                                                                              cACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACAttt                                      >  2:4516389/1‑150 (MQ=255)
                                                                                                              cACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACAttt                                      <  1:2225899/150‑1 (MQ=255)
                                                                                                                ccGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGTTATAGAACACGGTGGCCGGCATTTTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCTTTTACATTTTg                                    <  2:3203272/150‑1 (MQ=255)
                                                                                                                   cTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGAcc                                 <  2:1193873/150‑1 (MQ=255)
                                                                                                                       tGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAaca                             >  1:1163889/1‑150 (MQ=255)
                                                                                                                        gTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAacac                            <  2:1701958/150‑1 (MQ=255)
                                                                                                                            aCCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCg                        >  1:295759/1‑150 (MQ=255)
                                                                                                                            aCCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCg                        <  1:2027977/150‑1 (MQ=255)
                                                                                                                            aCCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCg                        <  1:3622475/150‑1 (MQ=255)
                                                                                                                               aGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCa                     <  2:2886653/150‑1 (MQ=255)
                                                                                                                                     gCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAgc               >  1:3368516/1‑150 (MQ=255)
                                                                                                                                     gCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAgc               >  1:2231030/1‑150 (MQ=255)
                                                                                                                                     gCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAgc               >  1:3571955/1‑150 (MQ=255)
                                                                                                                                     gCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAgc               >  1:1360988/1‑150 (MQ=255)
                                                                                                                                      cccGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAgcg              >  1:1931251/1‑150 (MQ=255)
                                                                                                                                      cccGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAgcg              <  2:721395/150‑1 (MQ=255)
                                                                                                                                           tttctttcATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGAt         >  1:4462424/1‑150 (MQ=255)
                                                                                                                                               tttcATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCat     <  1:3137342/150‑1 (MQ=255)
                                                                                                                                               tttcATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCat     >  2:4250829/1‑150 (MQ=255)
                                                                                                                                                  cATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCAtata  <  2:3676219/150‑1 (MQ=255)
                                                                                                                                                  cATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCAtata  <  1:776786/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
TCGCCATCAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATA  >  CP009273/2450186‑2450481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCGCCATCAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATA  >  CP009273/2450186‑2450481
                                                                                                                                                     |                                                                                                                                                  
TCGCCATCAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATT                                                                                                                                                    >  SRR19973080.2582864/1‑150 (MQ=60)
TCGCCATCAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATC                                                                                                                                                    >  SRR19973080.1432789/1‑150 (MQ=60)
TCGCCATCAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATA                                                                                                                                                    >  SRR19973080.1432845/1‑150 (MQ=60)
 CGCCATCAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTT                                                                                                                                                   >  SRR19973080.4222031/1‑150 (MQ=60)
  GCCATCAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTG                                                                                                                                                  <  SRR19973080.3523687/150‑1 (MQ=60)
   CCATCAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGA                                                                                                                                                 >  SRR19973080.1584182/1‑150 (MQ=60)
   CCATCAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGA                                                                                                                                                 >  SRR19973080.1582327/1‑150 (MQ=60)
      TCAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAAC                                                                                                                                              <  SRR19973080.790356/150‑1 (MQ=60)
       CAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACC                                                                                                                                             >  SRR19973080.450442/1‑150 (MQ=60)
           CGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGAT                                                                                                                                         <  SRR19973080.1680620/150‑1 (MQ=60)
           CGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGAT                                                                                                                                         <  SRR19973080.1682120/150‑1 (MQ=60)
            GAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATA                                                                                                                                        >  SRR19973080.3307594/1‑150 (MQ=60)
            GAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATA                                                                                                                                        >  SRR19973080.3307951/1‑150 (MQ=60)
              GATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAA                                                                                                                                      <  SRR19973080.310766/150‑1 (MQ=60)
               ATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAG                                                                                                                                     >  SRR19973080.1201059/1‑150 (MQ=60)
                    TCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAAC                                                                                                                                <  SRR19973080.2640367/150‑1 (MQ=60)
                    TCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAAC                                                                                                                                <  SRR19973080.603214/150‑1 (MQ=60)
                    TCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAAC                                                                                                                                <  SRR19973080.1047479/150‑1 (MQ=60)
                    TCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAAC                                                                                                                                <  SRR19973080.1924896/150‑1 (MQ=60)
                    TCACAACCATGAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAAC                                                                                                                                <  SRR19973080.2640357/150‑1 (MQ=60)
                            ATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCG                                                                                                                        >  SRR19973080.2243205/1‑150 (MQ=60)
                              TAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGC                                                                                                                      >  SRR19973080.4509642/1‑150 (MQ=60)
                                GTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCAT                                                                                                                    <  SRR19973080.3462978/150‑1 (MQ=60)
                                 TGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGGAAAAGGTGGCCGGCATT                                                                                                                   >  SRR19973080.4533613/1‑150 (MQ=60)
                                   GTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGT                                                                                                                 >  SRR19973080.4229989/1‑150 (MQ=60)
                                         GGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTA                                                                                                           <  SRR19973080.245533/150‑1 (MQ=60)
                                           ACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATC                                                                                                         >  SRR19973080.4406463/1‑150 (MQ=60)
                                              AACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCAT                                                                                                      <  SRR19973080.4533/150‑1 (MQ=60)
                                              AACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCAT                                                                                                      <  SRR19973080.2281710/150‑1 (MQ=60)
                                              AACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCAT                                                                                                      >  SRR19973080.1009870/1‑150 (MQ=60)
                                                CGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTC                                                                                                    >  SRR19973080.3464418/1‑150 (MQ=60)
                                                CGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTC                                                                                                    <  SRR19973080.4070499/150‑1 (MQ=60)
                                                       ACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAAT                                                                                             >  SRR19973080.4113164/1‑150 (MQ=60)
                                                         TGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGA                                                                                           <  SRR19973080.3864999/150‑1 (MQ=60)
                                                          GGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAA                                                                                          <  SRR19973080.2907902/150‑1 (MQ=60)
                                                          GGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAA                                                                                          <  SRR19973080.2907187/150‑1 (MQ=60)
                                                            CGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATAGTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAAC                                                                                        <  SRR19973080.1876395/150‑1 (MQ=60)
                                                               CATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCT                                                                                     >  SRR19973080.2155363/1‑150 (MQ=60)
                                                               CATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCT                                                                                     >  SRR19973080.2152739/1‑150 (MQ=60)
                                                                ATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAATGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTG                                                                                    >  SRR19973080.1518456/1‑150 (MQ=60)
                                                                ATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAATAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTG                                                                                    <  SRR19973080.3892857/150‑1 (MQ=60)
                                                                 TCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGT                                                                                   <  SRR19973080.2203420/150‑1 (MQ=60)
                                                                 TCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGT                                                                                   <  SRR19973080.4487588/150‑1 (MQ=60)
                                                                      GGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTT                                                                              <  SRR19973080.3366148/150‑1 (MQ=60)
                                                                       GCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTA                                                                             <  SRR19973080.4302354/150‑1 (MQ=60)
                                                                       GCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTA                                                                             <  SRR19973080.963673/150‑1 (MQ=60)
                                                                       GCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTA                                                                             <  SRR19973080.963530/150‑1 (MQ=60)
                                                                          GCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCT                                                                          <  SRR19973080.3446362/150‑1 (MQ=60)
                                                                          GCGTCGCCGTGACGCATGATAAAAACTTGCATAGTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCT                                                                          <  SRR19973080.3446388/150‑1 (MQ=60)
                                                                           CGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTC                                                                         <  SRR19973080.3583780/150‑1 (MQ=60)
                                                                              CGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAAT                                                                      <  SRR19973080.3638951/150‑1 (MQ=60)
                                                                                  GTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCG                                                                  <  SRR19973080.1405750/150‑1 (MQ=60)
                                                                                  GTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCG                                                                  <  SRR19973080.3212585/150‑1 (MQ=60)
                                                                                   TGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGT                                                                 <  SRR19973080.2046165/150‑1 (MQ=60)
                                                                                    GACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTA                                                                <  SRR19973080.1203695/150‑1 (MQ=60)
                                                                                    GACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTA                                                                <  SRR19973080.726950/150‑1 (MQ=60)
                                                                                    GACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTA                                                                <  SRR19973080.726885/150‑1 (MQ=60)
                                                                                     ACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAA                                                               >  SRR19973080.1212021/1‑150 (MQ=60)
                                                                                       GCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGT                                                             >  SRR19973080.1963743/1‑150 (MQ=60)
                                                                                       GCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGT                                                             >  SRR19973080.4252708/1‑150 (MQ=60)
                                                                                       GCATGATAAAAACTTGCATATTGCACCGATTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGT                                                             <  SRR19973080.746038/150‑1 (MQ=60)
                                                                                         ATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTAT                                                           <  SRR19973080.3448952/150‑1 (MQ=60)
                                                                                         ATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAAAGGTGGCCGGCATTGTGCCTTATGCATTCACCGAATGAAACGCTGTTTTTTAACTCAATTGCGTAAGTAT                                                           >  SRR19973080.3424043/1‑150 (MQ=60)
                                                                                         ATGATAAAAACTTGCATATTGCACCACTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTAT                                                           >  SRR19973080.2880215/1‑150 (MQ=60)
                                                                                            ATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGT                                                        >  SRR19973080.4082348/1‑150 (MQ=60)
                                                                                            ATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGT                                                        <  SRR19973080.4434310/150‑1 (MQ=60)
                                                                                            ATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCAGTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGT                                                        <  SRR19973080.4434318/150‑1 (MQ=60)
                                                                                             TAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTC                                                       <  SRR19973080.328936/150‑1 (MQ=60)
                                                                                             TAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTC                                                       <  SRR19973080.328887/150‑1 (MQ=60)
                                                                                             TAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTC                                                       <  SRR19973080.328162/150‑1 (MQ=60)
                                                                                             TAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTAACTCAATGGCGTAAGTATAGTC                                                       >  SRR19973080.1552105/1‑150 (MQ=60)
                                                                                              AAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCA                                                      >  SRR19973080.2980374/1‑150 (MQ=60)
                                                                                               AAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAA                                                     >  SRR19973080.4287042/1‑150 (MQ=60)
                                                                                               AAAACTTGCATATTACACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAA                                                     >  SRR19973080.1318427/1‑150 (MQ=60)
                                                                                                  ACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTTAGTATAGTCAATAT                                                  >  SRR19973080.1621683/1‑150 (MQ=60)
                                                                                                  ACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTAATTCCAATTAAACCAGAGAAAGAAAACGGTGGCCGGCCTTGTGGCTTATACATTAACCGAAATAAACGCTGGTTTTTTCCTCAATGGCGTAAGTATTGTCAATAT                                                  >  SRR19973080.848905/1‑150 (MQ=60)
                                                                                                   CTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATG                                                 >  SRR19973080.1124568/1‑150 (MQ=60)
                                                                                                      GCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGT                                              >  SRR19973080.3188386/1‑150 (MQ=60)
                                                                                                       CATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTT                                             >  SRR19973080.2970028/1‑150 (MQ=60)
                                                                                                         TATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTA                                           >  SRR19973080.4547346/1‑150 (MQ=60)
                                                                                                         TATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTA                                           >  SRR19973080.4547427/1‑150 (MQ=60)
                                                                                                            TGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACAT                                        <  SRR19973080.3010697/150‑1 (MQ=60)
                                                                                                            TGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACAT                                        <  SRR19973080.3383142/150‑1 (MQ=60)
                                                                                                             GCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATT                                       >  SRR19973080.2931224/1‑150 (MQ=60)
                                                                                                             GCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATT                                       >  SRR19973080.1714814/1‑150 (MQ=60)
                                                                                                             GCACCGCTTGTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATT                                       >  SRR19973080.1714929/1‑150 (MQ=60)
                                                                                                              CACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTT                                      <  SRR19973080.3866170/150‑1 (MQ=60)
                                                                                                              CACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTT                                      <  SRR19973080.2238164/150‑1 (MQ=60)
                                                                                                              CACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTTAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTT                                      <  SRR19973080.3089656/150‑1 (MQ=60)
                                                                                                                  GCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTAAATGGCGTAAGTATATTCAATATGCGTTTACATTTTTAC                                  >  SRR19973080.2432178/1‑150 (MQ=60)
                                                                                                                       TGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACA                             >  SRR19973080.1170795/1‑150 (MQ=60)
                                                                                                                          TAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTC                          >  SRR19973080.1477240/1‑150 (MQ=60)
                                                                                                                            ACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCG                        <  SRR19973080.2039500/150‑1 (MQ=60)
                                                                                                                            ACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCG                        >  SRR19973080.298570/1‑150 (MQ=60)
                                                                                                                            ACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCG                        >  SRR19973080.297645/1‑150 (MQ=60)
                                                                                                                            ACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCG                        <  SRR19973080.3642498/150‑1 (MQ=60)
                                                                                                                              CAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGTCCAACACTCCGCC                      >  SRR19973080.4077714/1‑150 (MQ=60)
                                                                                                                                 ATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATT                   <  SRR19973080.2540685/150‑1 (MQ=60)
                                                                                                                                   TCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCA                 >  SRR19973080.900915/1‑150 (MQ=60)
                                                                                                                                     GCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGC               >  SRR19973080.3180283/1‑150 (MQ=60)
                                                                                                                                     GCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGC               <  SRR19973080.4317701/150‑1 (MQ=60)
                                                                                                                                     GCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGC               >  SRR19973080.1369133/1‑150 (MQ=60)
                                                                                                                                     GCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGC               >  SRR19973080.3591714/1‑150 (MQ=60)
                                                                                                                                     GCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGC               >  SRR19973080.3591682/1‑150 (MQ=60)
                                                                                                                                     GCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGC               >  SRR19973080.3386776/1‑150 (MQ=60)
                                                                                                                                     GCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGC               >  SRR19973080.3180200/1‑150 (MQ=60)
                                                                                                                                     GCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGC               >  SRR19973080.2243314/1‑150 (MQ=60)
                                                                                                                                      CCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCG              >  SRR19973080.1942159/1‑150 (MQ=60)
                                                                                                                                        CGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCG            >  SRR19973080.166033/1‑150 (MQ=60)
                                                                                                                                          TTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGA          <  SRR19973080.3144056/150‑1 (MQ=60)
                                                                                                                                           TTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGAT         >  SRR19973080.4486606/1‑150 (MQ=60)
                                                                                                                                           TTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGAT         >  SRR19973080.397974/1‑150 (MQ=60)
                                                                                                                                               TTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCAT     <  SRR19973080.3154611/150‑1 (MQ=60)
                                                                                                                                                 TCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATAT   >  SRR19973080.1921338/1‑150 (MQ=60)
                                                                                                                                                  CATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATA  <  SRR19973080.781229/150‑1 (MQ=60)
                                                                                                                                                  CATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATA  <  SRR19973080.781230/150‑1 (MQ=60)
                                                                                                                                                  CATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATA  <  SRR19973080.1025178/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                  
TCGCCATCAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATA  >  CP009273/2450186‑2450481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑