Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F98 I0 R1	799	294.1	9752082	92.2%	8991419	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,450,335	C→T	73.7%	intergenic (‑44/+159)	sixA ← / ← fadJ	phosphohistidine phosphatase/fused enoyl‑CoA hydratase and epimerase and isomerase/3‑hydroxyacyl‑CoA dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,450,335	0	C	T	73.7%	449.7 / 178.1	246	intergenic (‑44/+159)	sixA/fadJ	phosphohistidine phosphatase/fused enoyl‑CoA hydratase and epimerase and isomerase/3‑hydroxyacyl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand): ref base C (26/38); new base T (84/96); total (112/134)
Fisher's exact test for biased strand distribution p-value = 4.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 247 total aligned reads displayed.

CGCCATCAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCT  >  CP009273/2450187‑2450484

 gCCATCAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTg                                                                                                                                                     >  1:3790237/1‑150 (MQ=255)

 gCCATCAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTg                                                                                                                                                     >  2:3056537/1‑150 (MQ=255)

  ccATCAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGa                                                                                                                                                    >  2:451817/1‑150 (MQ=255)

   cATCAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGaa                                                                                                                                                   <  1:1061689/150‑1 (MQ=255)

   cATCAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGaa                                                                                                                                                   >  2:2243275/1‑150 (MQ=255)

   cATCAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGaa                                                                                                                                                   >  1:1537613/1‑150 (MQ=255)

   cATCAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGaa                                                                                                                                                   <  1:4525869/150‑1 (MQ=255)

   cATCAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGaa                                                                                                                                                   >  2:752946/1‑150 (MQ=255)

     tCAGGCGAGATTCGTCACAACCATGAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAAc                                                                                                                                                 <  2:3617522/150‑1 (MQ=255)

               ttCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGataaaga                                                                                                                                       >  1:2045964/1‑150 (MQ=255)

                 cGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGAtaaagaaa                                                                                                                                     <  1:4677620/150‑1 (MQ=255)

                 cGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGAtaaagaaa                                                                                                                                     <  2:4729643/150‑1 (MQ=255)

                  gTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGaaaa                                                                                                                                    >  2:2376473/1‑150 (MQ=255)

                   tCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAAc                                                                                                                                   <  1:2722794/150‑1 (MQ=255)

                   tCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAAc                                                                                                                                   <  2:4477066/150‑1 (MQ=255)

                     acaACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACgg                                                                                                                                 >  1:2751422/1‑150 (MQ=255)

                     acaACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACgg                                                                                                                                 <  2:1432294/150‑1 (MQ=255)

                      caACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGt                                                                                                                                <  1:4683978/150‑1 (MQ=255)

                       aaCCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTg                                                                                                                               <  1:3941392/150‑1 (MQ=255)

                          cATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGcc                                                                                                                            <  1:2721055/150‑1 (MQ=255)

                            ttAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCgg                                                                                                                          >  2:579692/1‑150 (MQ=255)

                              aGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCa                                                                                                                        <  2:2296780/150‑1 (MQ=255)

                               gTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCAt                                                                                                                       <  1:528835/150‑1 (MQ=255)

                               gTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCAt                                                                                                                       <  1:1156917/150‑1 (MQ=255)

                                tGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCAtt                                                                                                                      <  2:3291180/150‑1 (MQ=255)

                                 ggTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATtg                                                                                                                     >  1:1633192/1‑150 (MQ=255)

                                   tCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGAGTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGACAACGGTGGCCGGCATtgtg                                                                                                                   >  2:901941/1‑150 (MQ=255)

                                    cAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGc                                                                                                                  >  1:3520335/1‑150 (MQ=255)

                                      taggttgAACGGAATCACTTGCTGCATCGATTGCTGAGTCGACTTTACGCATGATATAATCTTTCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACAAGATAAAGAAAACGGTGGCCGGCATTGTGCCt                                                                                                                <  2:1822476/144‑1 (MQ=255)

                                        ggACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAAAGGTGGCCGGCATTGTGCCTTa                                                                                                              >  1:4171568/1‑150 (MQ=255)

                                            gAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAGAGAAAACGGTGGCCGGCATTGTGCCTTATCCa                                                                                                          >  2:1274521/1‑150 (MQ=255)

                                            gAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCa                                                                                                          >  2:712483/1‑150 (MQ=255)

                                             aaCGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCAGTGTGCCTTATccct                                                                                                         >  1:723762/1‑148 (MQ=255)

                                                ggAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCa                                                                                                      >  1:1003343/1‑150 (MQ=255)

                                                ggAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCa                                                                                                      >  2:1170987/1‑150 (MQ=255)

                                                ggAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCa                                                                                                      <  2:2042774/150‑1 (MQ=255)

                                                  aaTCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCAcc                                                                                                    <  2:4872494/150‑1 (MQ=255)

                                                     cACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGaa                                                                                                 <  1:1382145/150‑1 (MQ=255)

                                                      aCTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAAt                                                                                                >  2:2947154/1‑150 (MQ=255)

                                                      aCTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAAt                                                                                                >  2:678697/1‑150 (MQ=255)

                                                       cTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATACAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATg                                                                                               <  2:1556517/150‑1 (MQ=255)

                                                       cTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATg                                                                                               <  1:750610/150‑1 (MQ=255)

                                                         ggcggcATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGaa                                                                                             >  2:4657680/1‑150 (MQ=255)

                                                          gcggcATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGaaa                                                                                            <  1:4407043/150‑1 (MQ=255)

                                                              cATCGAGGGCTGCGTCGCCGTGACGCATGATAAAATCTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCt                                                                                        <  1:954100/150‑1 (MQ=255)

                                                                tCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGt                                                                                      <  1:2258443/150‑1 (MQ=255)

                                                                  gAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGttt                                                                                    <  1:1833897/150‑1 (MQ=255)

                                                                   aGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTATTTAAACTGAAACCAGAGAAAGAAAACGGGGGCCGGCCTAGTGCCGTATCCAATCACCGAATGAAACGCTGtttt                                                                                   >  1:1295874/1‑150 (MQ=255)

                                                                       cTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTAc                                                                               <  2:1443016/150‑1 (MQ=255)

                                                                        tGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTAcc                                                                              <  2:3139460/150‑1 (MQ=255)

                                                                         gCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCt                                                                             <  1:518905/150‑1 (MQ=255)

                                                                         gCGTCGCCGTGACGCATGATAAAAACTTGAATATTGCACCGCTTTTGTTAACCAGATTCGCCCTTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCt                                                                             <  1:4255140/150‑1 (MQ=255)

                                                                             cGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTTCCTCAAt                                                                         >  2:4565777/1‑150 (MQ=255)

                                                                               ccGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATgg                                                                       <  2:3424201/150‑1 (MQ=255)

                                                                                cGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGc                                                                      >  2:4157402/1‑150 (MQ=255)

                                                                                       cATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGta                                                               <  1:4851322/150‑1 (MQ=255)

                                                                                        aTGATAAAAACTTGCATAGTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGtat                                                              <  2:1851067/150‑1 (MQ=255)

                                                                                         tGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGtata                                                             <  2:2514277/150‑1 (MQ=255)

                                                                                         tGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGtata                                                             <  2:3885647/150‑1 (MQ=255)

                                                                                            tAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTc                                                          >  1:4267563/1‑150 (MQ=255)

                                                                                             aaaaaCTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCa                                                         <  1:1630800/150‑1 (MQ=255)

                                                                                             aaaaaCTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCa                                                         >  1:1999076/1‑150 (MQ=255)

                                                                                             aaaaaCTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCa                                                         <  2:4815717/150‑1 (MQ=255)

                                                                                             aaaaaCTTGCAGATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCa                                                         <  2:1564718/150‑1 (MQ=255)

                                                                                                 aCTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAatat                                                     <  2:1747139/150‑1 (MQ=255)

                                                                                                  cTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATg                                                    >  2:4171815/1‑150 (MQ=255)

                                                                                                  cTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGACACCAGAGAAAGAAAACGGTGGCCGGCCTTGTGCCTTATCCATTAACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATg                                                    >  2:2657662/1‑150 (MQ=255)

                                                                                                      cATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGtt                                                <  1:298951/150‑1 (MQ=255)

                                                                                                       atatTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGttt                                               >  1:3902645/1‑150 (MQ=255)

                                                                                                        tatTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTTCCTCAATGGCGTAAGTATAGTCAATATGCGTTTa                                              >  2:4845986/1‑150 (MQ=255)

                                                                                                          ttGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACAAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATACATTCACCGAATGAAACGATGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTAACa                                            >  2:1561787/1‑150 (MQ=255)

                                                                                                            gCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACAtt                                          <  1:926400/150‑1 (MQ=255)

                                                                                                                 gCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGAc                                     <  1:1161745/150‑1 (MQ=255)

                                                                                                                   ttttGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCa                                   >  2:2634233/1‑150 (MQ=255)

                                                                                                                    tttGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCaa                                  >  1:3287044/1‑150 (MQ=255)

                                                                                                                        ttAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACt                              >  1:974753/1‑150 (MQ=255)

                                                                                                                        ttAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACt                              <  2:1529124/150‑1 (MQ=255)

                                                                                                                        ttAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACt                              >  1:3416705/1‑150 (MQ=255)

                                                                                                                          aaCCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTcc                            <  2:2575335/150‑1 (MQ=255)

                                                                                                                          aaCCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTcc                            >  1:2163309/1‑150 (MQ=255)

                                                                                                                          aaCCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTcc                            <  1:3056537/150‑1 (MQ=255)

                                                                                                                          aaCCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTcc                            <  1:3376204/150‑1 (MQ=255)

                                                                                                                               gATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCAt                       >  2:2746691/1‑150 (MQ=255)

                                                                                                                                aTTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCAtt                      >  2:2195330/1‑150 (MQ=255)

                                                                                                                                   cGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAg                   >  1:936258/1‑150 (MQ=255)

                                                                                                                                    gCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAgc                  >  1:3304286/1‑150 (MQ=255)

                                                                                                                                     cccGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAgcg                 >  1:442358/1‑150 (MQ=255)

                                                                                                                                       cGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGcgcg               <  1:2356847/150‑1 (MQ=255)

                                                                                                                                        gTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCgg              >  2:1387301/1‑150 (MQ=255)

                                                                                                                                        gTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCgg              >  2:3844520/1‑150 (MQ=255)

                                                                                                                                         ttttCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGa             <  2:1657925/150‑1 (MQ=255)

                                                                                                                                         ttttCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGa             <  2:930116/150‑1 (MQ=255)

                                                                                                                                           ttctttcATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGAtt           <  2:3992114/150‑1 (MQ=255)

                                                                                                                                           ttctttcATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGAtt           <  1:186047/150‑1 (MQ=255)

                                                                                                                                            tctttcATTAGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGAGTc          >  1:616096/1‑150 (MQ=255)

                                                                                                                                             ctttcATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCa         <  2:43339/150‑1 (MQ=255)

                                                                                                                                             ctttcATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCa         >  1:2679099/1‑150 (MQ=255)

                                                                                                                                               ttcATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCata       >  1:688448/1‑150 (MQ=255)

                                                                                                                                                 cATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCAtata     >  1:2319035/1‑150 (MQ=255)

                                                                                                                                                    cTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCt  >  1:2858888/1‑150 (MQ=255)

CGCCATCAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCT  >  CP009273/2450187‑2450484

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TCGCCATCAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCT  >  CP009273/2450186‑2450484

TCGCCATCAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATT                                                                                                                                                       <  SRR19973081.494955/150‑1 (MQ=60)

TCGCCATCAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATT                                                                                                                                                       <  SRR19973081.494861/150‑1 (MQ=60)

 CGCCATCAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCT                                                                                                                                                      <  SRR19973081.902204/150‑1 (MQ=60)

  GCCATCAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTG                                                                                                                                                     >  SRR19973081.1335727/1‑150 (MQ=60)

  GCCATCAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTG                                                                                                                                                     >  SRR19973081.3813521/1‑150 (MQ=60)

   CCATCAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGA                                                                                                                                                    >  SRR19973081.1539130/1‑150 (MQ=60)

    CATCAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAA                                                                                                                                                   >  SRR19973081.294681/1‑150 (MQ=60)

    CATCAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAA                                                                                                                                                   >  SRR19973081.1547733/1‑150 (MQ=60)

    CATCAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAA                                                                                                                                                   >  SRR19973081.1547645/1‑150 (MQ=60)

    CATCAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAA                                                                                                                                                   <  SRR19973081.2761635/150‑1 (MQ=60)

    CATCAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAA                                                                                                                                                   <  SRR19973081.2037161/150‑1 (MQ=60)

    CATCAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAA                                                                                                                                                   <  SRR19973081.1068784/150‑1 (MQ=60)

    CATCAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAA                                                                                                                                                   <  SRR19973081.4553265/150‑1 (MQ=60)

    CATCAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAA                                                                                                                                                   <  SRR19973081.2083458/150‑1 (MQ=60)

       CAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACC                                                                                                                                                <  SRR19973081.3954735/150‑1 (MQ=60)

         GGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAG                                                                                                                                              >  SRR19973081.3158641/1‑150 (MQ=60)

           CGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGAT                                                                                                                                            <  SRR19973081.4799676/150‑1 (MQ=60)

               ATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAG                                                                                                                                        <  SRR19973081.3914431/150‑1 (MQ=60)

                TTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGA                                                                                                                                       >  SRR19973081.2058848/1‑150 (MQ=60)

                  CGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAA                                                                                                                                     <  SRR19973081.2867274/150‑1 (MQ=60)

                  CGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAA                                                                                                                                     <  SRR19973081.4050396/150‑1 (MQ=60)

                  CGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAA                                                                                                                                     <  SRR19973081.4704362/150‑1 (MQ=60)

                  CGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAA                                                                                                                                     <  SRR19973081.4706002/150‑1 (MQ=60)

                  CGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAA                                                                                                                                     <  SRR19973081.1575477/150‑1 (MQ=60)

                    TCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAAC                                                                                                                                   <  SRR19973081.2739610/150‑1 (MQ=60)

                      ACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGG                                                                                                                                 >  SRR19973081.2768343/1‑150 (MQ=60)

                       CAACCATTAGTGGTCAGAGGACTAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGAGTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGT                                                                                                                                <  SRR19973081.3310903/150‑1 (MQ=60)

                       CAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGT                                                                                                                                <  SRR19973081.4712346/150‑1 (MQ=60)

                       CAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGT                                                                                                                                <  SRR19973081.4712391/150‑1 (MQ=60)

                       CAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGT                                                                                                                                <  SRR19973081.4712385/150‑1 (MQ=60)

                        AACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTG                                                                                                                               <  SRR19973081.3965543/150‑1 (MQ=60)

                        AACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTG                                                                                                                               <  SRR19973081.1238993/150‑1 (MQ=60)

                           CATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCC                                                                                                                            <  SRR19973081.2737860/150‑1 (MQ=60)

                                GTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCAT                                                                                                                       <  SRR19973081.1164379/150‑1 (MQ=60)

                                GTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCAT                                                                                                                       <  SRR19973081.532600/150‑1 (MQ=60)

                                  GGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTG                                                                                                                     >  SRR19973081.1643803/1‑150 (MQ=60)

                                  GGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTG                                                                                                                     >  SRR19973081.4306310/1‑150 (MQ=60)

                                     CAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGC                                                                                                                  >  SRR19973081.3541870/1‑150 (MQ=60)

                                         GGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAAAGGTGGCCGGCATTGTGCCTTA                                                                                                              >  SRR19973081.4197073/1‑150 (MQ=60)

                                              AACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCAGTGTGCCTTATCCCT                                                                                                         >  SRR19973081.728548/1‑150 (MQ=60)

                                                 GGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCA                                                                                                      >  SRR19973081.1010146/1‑150 (MQ=60)

                                                 GGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCA                                                                                                      >  SRR19973081.1932580/1‑150 (MQ=60)

                                                      CACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAA                                                                                                 <  SRR19973081.1391413/150‑1 (MQ=60)

                                                        CTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATG                                                                                               <  SRR19973081.755490/150‑1 (MQ=60)

                                                        CGGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATG                                                                                               <  SRR19973081.755450/150‑1 (MQ=60)

                                                         TGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGA                                                                                              <  SRR19973081.3856906/150‑1 (MQ=60)

                                                           GCGGCATCGATGGCTGCGTCGCAGTGACGAATGATAAAAAATTGCATATTGCACCGCTTTTTTTAACCAGTTTCGCCCGTTTTCTTTCATTTGAAACAAGATAAAGAAAACGGTGGCCGTCATTGTGCCTTATACATTCACCGAATGAAA                                                                                            <  SRR19973081.4187248/150‑1 (MQ=60)

                                                           GCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAA                                                                                            <  SRR19973081.1967056/150‑1 (MQ=60)

                                                           GCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAA                                                                                            <  SRR19973081.4434411/150‑1 (MQ=60)

                                                           GCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAA                                                                                            <  SRR19973081.4433586/150‑1 (MQ=60)

                                                               CATCGAGGGCTGCGTCGCCGTGACGCATGATAAAATCTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCT                                                                                        <  SRR19973081.960564/150‑1 (MQ=60)

                                                                 TCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGT                                                                                      <  SRR19973081.2272714/150‑1 (MQ=60)

                                                                 TCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGGTTTATTTAATATGAAAACAGAGAAAGAAAAAGGTGGCCGGCCTTGTGCCGTATCCAATCACCGAATGAAAAGCTGT                                                                                      >  SRR19973081.3033016/1‑150 (MQ=60)

                                                                  CGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTT                                                                                     >  SRR19973081.3374562/1‑150 (MQ=60)

                                                                  CGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTT                                                                                     >  SRR19973081.3095981/1‑150 (MQ=60)

                                                                   GAGGGCTGCGTCGCCGTGACGCTTGATAAAATCTTGCATATTGCACCGCTTTTTTTAACCTGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTT                                                                                    <  SRR19973081.1846064/150‑1 (MQ=60)

                                                                   GAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTT                                                                                    <  SRR19973081.1845819/150‑1 (MQ=60)

                                                                    AGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTATTTAAACTGAAACCAGAGAAAGAAAACGGGGGCCGGCCTAGTGCCGTATCCAATCACCGAATGAAACGCTGTTTT                                                                                   >  SRR19973081.1304507/1‑150 (MQ=60)

                                                                        CTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTAC                                                                               <  SRR19973081.1165638/150‑1 (MQ=60)

                                                                        CTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTAC                                                                               <  SRR19973081.1165296/150‑1 (MQ=60)

                                                                         TGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACC                                                                              <  SRR19973081.775644/150‑1 (MQ=60)

                                                                         TGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAAACAGAGAAAGAAAACGGTGGCCCGCATTTTGCCATATCCAATAACCGAATGAAACGCAGTTTTTTAAC                                                                              >  SRR19973081.3457790/1‑150 (MQ=60)

                                                                          GCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCT                                                                             <  SRR19973081.522598/150‑1 (MQ=60)

                                                                          GCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCT                                                                             <  SRR19973081.522515/150‑1 (MQ=60)

                                                                          GCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCT                                                                             <  SRR19973081.521672/150‑1 (MQ=60)

                                                                          GCGTCGCCGTGACGCATGATAAAAACTTGAATATTGCACCGCTTTTGTTAACCAGATTCGCCCTTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCT                                                                             <  SRR19973081.4281115/150‑1 (MQ=60)

                                                                           CGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTATTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTC                                                                            >  SRR19973081.4330555/1‑150 (MQ=60)

                                                                            GTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCA                                                                           >  SRR19973081.2154092/1‑150 (MQ=60)

                                                                                 CGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGC                                                                      <  SRR19973081.4454451/150‑1 (MQ=60)

                                                                                    GACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTA                                                                   <  SRR19973081.644968/150‑1 (MQ=60)

                                                                                      CGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAG                                                                 <  SRR19973081.139050/150‑1 (MQ=60)

                                                                                        CATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTA                                                               <  SRR19973081.4880444/150‑1 (MQ=60)

                                                                                         ATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGAGTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTAT                                                              <  SRR19973081.3589214/150‑1 (MQ=60)

                                                                                           GATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAATAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGACTGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAG                                                            >  SRR19973081.3436287/1‑150 (MQ=60)

                                                                                             TAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTC                                                          >  SRR19973081.4293595/1‑150 (MQ=60)

                                                                                             TAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTC                                                          >  SRR19973081.4293361/1‑150 (MQ=60)

                                                                                              AAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCA                                                         >  SRR19973081.2011561/1‑150 (MQ=60)

                                                                                              AAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCA                                                         <  SRR19973081.1641395/150‑1 (MQ=60)

                                                                                              AAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCA                                                         <  SRR19973081.1641387/150‑1 (MQ=60)

                                                                                                 AACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATA                                                      <  SRR19973081.271361/150‑1 (MQ=60)

                                                                                                 AACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATA                                                      <  SRR19973081.271118/150‑1 (MQ=60)

                                                                                                  ACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTATTTCATATTAAACAAGAGAAAAAAAAAGGTGGgccgcattttgacgtaaacattcaacaaaataaaaggtgtttttttgctcaaatgcggaagtatattaaaaat                                                     >  SRR19973081.1740542/1‑77 (MQ=58)

                                                                                                   CTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATG                                                    <  SRR19973081.974539/150‑1 (MQ=60)

                                                                                                       CATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTT                                                >  SRR19973081.2748627/1‑150 (MQ=60)

                                                                                                       CATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTT                                                <  SRR19973081.301195/150‑1 (MQ=60)

                                                                                                       CATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTATTTCATATGAAACCAGAGAAAGAAAAAGGTGGCCGGCATTGTGCCTTATACATTCACCGAATGAAACGCTGTTTTTTTACTCAAAGGCGTAAGTATATTAAATATTCGTT                                                >  SRR19973081.701096/1‑150 (MQ=60)

                                                                                                       CATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTATTTAATCTTAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCAATCACCCAATGAAACGCTGTTTTTTACCTCAAAGTCGTAAGTATAGTAAATATGAGTT                                                >  SRR19973081.701016/1‑150 (MQ=60)

                                                                                                        ATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTT                                               >  SRR19973081.3926610/1‑150 (MQ=60)

                                                                                                           TTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTAAA                                            >  SRR19973081.2583550/1‑150 (MQ=60)

                                                                                                            TGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACAT                                           >  SRR19973081.4518201/1‑150 (MQ=60)

                                                                                                             GCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATT                                          <  SRR19973081.932635/150‑1 (MQ=60)

                                                                                                                  GCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGAC                                     >  SRR19973081.2879137/1‑150 (MQ=60)

                                                                                                                  GCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGAC                                     >  SRR19973081.1734399/1‑150 (MQ=60)

                                                                                                                  GCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGAC                                     <  SRR19973081.1169226/150‑1 (MQ=60)

                                                                                                                  GCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGAC                                     <  SRR19973081.1056366/150‑1 (MQ=60)

                                                                                                                   CTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACC                                    >  SRR19973081.1118991/1‑150 (MQ=60)

                                                                                                                    TTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAATAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCA                                   <  SRR19973081.533308/150‑1 (MQ=60)

                                                                                                                    TTTTGTTAACCAGAGTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCA                                   <  SRR19973081.533001/150‑1 (MQ=60)

                                                                                                                     TTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAA                                  >  SRR19973081.3307054/1‑150 (MQ=60)

                                                                                                                       TGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACA                                >  SRR19973081.1758404/1‑150 (MQ=60)

                                                                                                                         TTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACT                              >  SRR19973081.981382/1‑150 (MQ=60)

                                                                                                                         TTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACT                              >  SRR19973081.3437721/1‑150 (MQ=60)

                                                                                                                         TTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACT                              <  SRR19973081.2737977/150‑1 (MQ=60)

                                                                                                                           AACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCC                            >  SRR19973081.2177087/1‑150 (MQ=60)

                                                                                                                           AACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCC                            <  SRR19973081.3396958/150‑1 (MQ=60)

                                                                                                                           AACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCC                            <  SRR19973081.3075346/150‑1 (MQ=60)

                                                                                                                           AACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCC                            <  SRR19973081.940114/150‑1 (MQ=60)

                                                                                                                                 ATTCGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATT                      >  SRR19973081.2342090/1‑150 (MQ=60)

                                                                                                                                 ATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATT                      <  SRR19973081.3511467/150‑1 (MQ=60)

                                                                                                                                    CGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAG                   >  SRR19973081.4197965/1‑150 (MQ=60)

                                                                                                                                    CGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAG                   >  SRR19973081.943372/1‑150 (MQ=60)

                                                                                                                                    CGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAG                   >  SRR19973081.942576/1‑150 (MQ=60)

                                                                                                                                    CGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAG                   >  SRR19973081.942574/1‑150 (MQ=60)

                                                                                                                                    CGCCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTACGCCATTCAG                   >  SRR19973081.1003950/1‑150 (MQ=60)

                                                                                                                                     GCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATATTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGC                  >  SRR19973081.3325554/1‑150 (MQ=60)

                                                                                                                                     GCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGC                  >  SRR19973081.3324461/1‑150 (MQ=60)

                                                                                                                                      CCCGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCG                 >  SRR19973081.445367/1‑150 (MQ=60)

                                                                                                                                        CGTTTTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCG               <  SRR19973081.2227522/150‑1 (MQ=60)

                                                                                                                                        CGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCG               <  SRR19973081.2371502/150‑1 (MQ=60)

                                                                                                                                            TTCTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATT           <  SRR19973081.187698/150‑1 (MQ=60)

                                                                                                                                             TCTTTCATTAGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGAGTC          >  SRR19973081.620402/1‑150 (MQ=60)

                                                                                                                                              CTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCA         >  SRR19973081.2695722/1‑150 (MQ=60)

                                                                                                                                              CTTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCA         <  SRR19973081.2289915/150‑1 (MQ=60)

                                                                                                                                               TTTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCAT        >  SRR19973081.522708/1‑150 (MQ=60)

                                                                                                                                                TTCATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATA       >  SRR19973081.693075/1‑150 (MQ=60)

                                                                                                                                                  CATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATA     >  SRR19973081.2333552/1‑150 (MQ=60)

                                                                                                                                                  CATTTGAAACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATA     >  SRR19973081.2333548/1‑150 (MQ=60)

                                                                                                                                                     CTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCT  >  SRR19973081.2876325/1‑150 (MQ=60)

TCGCCATCAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCT  >  CP009273/2450186‑2450484

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`