Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F534 I1 R1 1235 230.2 7611948 95.1% 7238962 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 2,450,335 C→T intergenic (‑44/+159) sixA ← / ← fadJ phosphohistidine phosphatase/fused enoyl‑CoA hydratase and epimerase and isomerase/3‑hydroxyacyl‑CoA dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,450,3350CT99.6% 752.8 / ‑4.7 224intergenic (‑44/+159)sixA/fadJphosphohistidine phosphatase/fused enoyl‑CoA hydratase and epimerase and isomerase/3‑hydroxyacyl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (99/124);  total (100/124)
Fisher's exact test for biased strand distribution p-value = 4.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 224 total aligned reads displayed.

CCATCAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATA  >  CP009273/2450189‑2450479
                                                                                                                                                  |                                                                                                                                                
ccATCAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGa                                                                                                                                               >  1:562904/1‑150 (MQ=255)
ccATCAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGa                                                                                                                                               >  2:2739907/1‑150 (MQ=255)
 cATCAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGat                                                                                                                                              <  1:96709/150‑2 (MQ=255)
    cAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATAcc                                                                                                                                           >  2:1651529/1‑150 (MQ=255)
     aGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCa                                                                                                                                          <  1:233792/150‑1 (MQ=255)
          agaTTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGataa                                                                                                                                     <  2:1436280/150‑1 (MQ=255)
            aTTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGataaag                                                                                                                                   <  2:511068/150‑1 (MQ=255)
            aTTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCAATTGATACCAGataaag                                                                                                                                   >  1:272537/1‑150 (MQ=255)
                  cacaACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACg                                                                                                                             >  2:3307114/1‑150 (MQ=255)
                  cacaACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACg                                                                                                                             <  1:3233785/150‑1 (MQ=255)
                  cacaACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACg                                                                                                                             >  1:2265020/1‑150 (MQ=255)
                  cacaACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACg                                                                                                                             <  1:2862082/150‑1 (MQ=255)
                    caACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAACGAAAACGGt                                                                                                                           <  2:1981879/150‑1 (MQ=255)
                      aCCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTgg                                                                                                                         <  2:577173/150‑1 (MQ=255)
                      aCCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCCGCGTCGCCGTGACGCATGATAAAAACTTGCAGATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTgg                                                                                                                         <  1:3052533/150‑1 (MQ=255)
                        cATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGcc                                                                                                                       <  1:1706661/150‑1 (MQ=255)
                         aTTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCg                                                                                                                      <  2:295371/150‑1 (MQ=255)
                            aGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGTGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCa                                                                                                                   >  1:1085234/1‑150 (MQ=255)
                            aGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCa                                                                                                                   >  2:286521/1‑150 (MQ=255)
                                 tCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATtgtg                                                                                                              >  2:2424505/1‑150 (MQ=255)
                                    gagGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGcca                                                                                                           <  2:2607078/150‑2 (MQ=255)
                                    gagGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCt                                                                                                           <  2:2278463/150‑1 (MQ=255)
                                       gACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTAt                                                                                                        >  2:2225991/1‑150 (MQ=255)
                                        aCGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATc                                                                                                       >  2:668763/1‑150 (MQ=255)
                                        aCGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATc                                                                                                       <  1:1286489/150‑1 (MQ=255)
                                         cGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATcc                                                                                                      >  2:147547/1‑150 (MQ=255)
                                         cGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATcc                                                                                                      >  2:2061789/1‑150 (MQ=255)
                                         cGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATcc                                                                                                      <  1:194003/150‑1 (MQ=255)
                                          gAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCa                                                                                                     <  2:1485536/150‑1 (MQ=255)
                                               gAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCAc                                                                                                <  1:2930918/150‑1 (MQ=255)
                                                aaTCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCAcc                                                                                               <  1:3349686/150‑1 (MQ=255)
                                                  tCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGa                                                                                             <  2:3786864/150‑1 (MQ=255)
                                                    aCTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAAt                                                                                           >  1:2787976/1‑150 (MQ=255)
                                                    aCTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAAt                                                                                           <  2:1870769/150‑1 (MQ=255)
                                                      tGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGa                                                                                         >  2:762285/1‑150 (MQ=255)
                                                         cggcATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAAc                                                                                      >  2:1192727/1‑150 (MQ=255)
                                                          ggcATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACg                                                                                     <  1:859567/150‑1 (MQ=255)
                                                             aTCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTg                                                                                  <  2:1372507/150‑1 (MQ=255)
                                                             aTCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTg                                                                                  <  2:371255/150‑1 (MQ=255)
                                                              tCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGt                                                                                 <  1:998026/150‑1 (MQ=255)
                                                                gAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGttt                                                                               <  2:251219/150‑1 (MQ=255)
                                                                  gggCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGttttt                                                                             <  2:2265020/150‑1 (MQ=255)
                                                                    gCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTa                                                                           <  2:1512428/150‑1 (MQ=255)
                                                                     cTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTAc                                                                          >  1:1438695/1‑150 (MQ=255)
                                                                         gTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCa                                                                      <  2:2138444/150‑1 (MQ=255)
                                                                         gTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCa                                                                      >  2:2313005/1‑150 (MQ=255)
                                                                           cGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAAt                                                                    <  1:836218/150‑1 (MQ=255)
                                                                             ccGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATgg                                                                  <  2:1756506/150‑1 (MQ=255)
                                                                             ccGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATgg                                                                  <  1:287534/150‑1 (MQ=255)
                                                                               gTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCg                                                                <  1:2861273/150‑1 (MQ=255)
                                                                                 gACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTa                                                              >  1:3055253/1‑150 (MQ=255)
                                                                                 gACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTa                                                              >  1:1625191/1‑150 (MQ=255)
                                                                                  aCGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTaa                                                             >  2:3187010/1‑150 (MQ=255)
                                                                                      aTGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGtat                                                         >  1:1473512/1‑150 (MQ=255)
                                                                                      aTGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGtat                                                         >  1:3574966/1‑150 (MQ=255)
                                                                                         aTAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGt                                                      >  1:843850/1‑150 (MQ=255)
                                                                                         aTAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGt                                                      >  1:104778/1‑150 (MQ=255)
                                                                                         aTAAAAACTTGCATAGTGCACCGCTTTTGTGAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGt                                                      <  1:3269269/150‑1 (MQ=255)
                                                                                               aCTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAatat                                                <  1:3606371/150‑1 (MQ=255)
                                                                                               aCTTGCATAGTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAatat                                                <  1:372357/150‑1 (MQ=255)
                                                                                                cTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATg                                               <  2:3172730/150‑1 (MQ=255)
                                                                                                cTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATg                                               >  2:2180898/1‑150 (MQ=255)
                                                                                                cTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATg                                               <  2:1889177/150‑1 (MQ=255)
                                                                                                  tGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCg                                             >  1:773944/1‑150 (MQ=255)
                                                                                                  tGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCg                                             <  1:147555/150‑1 (MQ=255)
                                                                                                  tGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCg                                             <  1:2061789/150‑1 (MQ=255)
                                                                                                   gCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGt                                            >  1:2302883/1‑150 (MQ=255)
                                                                                                    cATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGtt                                           >  2:1942297/1‑150 (MQ=255)
                                                                                                    cATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGtt                                           <  2:3462919/150‑1 (MQ=255)
                                                                                                    cATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGtt                                           >  1:1841373/1‑150 (MQ=255)
                                                                                                     atatTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGttt                                          <  2:2651029/150‑1 (MQ=255)
                                                                                                      tatTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTa                                         >  2:3282730/1‑150 (MQ=255)
                                                                                                       atTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTAc                                        >  2:120408/1‑150 (MQ=255)
                                                                                                         tGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACAt                                      <  1:3386181/150‑1 (MQ=255)
                                                                                                             ccGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTg                                  <  1:3410749/150‑1 (MQ=255)
                                                                                                             ccGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTg                                  <  1:2225991/150‑1 (MQ=255)
                                                                                                             ccGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTCACATTTTg                                  <  2:3447233/150‑1 (MQ=255)
                                                                                                               gCTTTTGTTAACCAGATTCGCCCGTTATCTTTCATTTGATAACAGATAAAGAAAACGGTGGCCGGCATAGTGCCTTATCCATTCAACGAATGAAACGCAGTTTTTTACCTCAATGGCGTAAAAATAGTCAATATGCGTTTACATTTTGAc                                >  1:1679731/1‑150 (MQ=255)
                                                                                                                cTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGAcc                               >  1:3252380/1‑150 (MQ=255)
                                                                                                                   ttGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAac                            >  1:576920/1‑150 (MQ=255)
                                                                                                                   ttGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAac                            <  1:529302/150‑1 (MQ=255)
                                                                                                                   ttGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAac                            <  1:2436068/150‑1 (MQ=255)
                                                                                                                    tGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAaca                           >  1:2287461/1‑150 (MQ=255)
                                                                                                                      ttAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACt                         <  2:3060785/150‑1 (MQ=255)
                                                                                                                       tAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTc                        >  2:3788333/1‑150 (MQ=255)
                                                                                                                       tAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTc                        >  2:2972010/1‑150 (MQ=255)
                                                                                                                         aCCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTTAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCg                      <  2:1881391/150‑1 (MQ=255)
                                                                                                                          ccAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGc                     >  2:1594730/1‑150 (MQ=255)
                                                                                                                             gATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCAt                  >  1:3456466/1‑150 (MQ=255)
                                                                                                                                tCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCa               >  1:1171039/1‑150 (MQ=255)
                                                                                                                                  gCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAgc             >  2:2215131/1‑150 (MQ=255)
                                                                                                                                     cGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGcgcg          >  1:513649/1‑150 (MQ=255)
                                                                                                                                     cGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGcgcg          <  1:1060586/150‑1 (MQ=255)
                                                                                                                                      gTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCgg         >  1:2586347/1‑150 (MQ=255)
                                                                                                                                        tttctttcATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGAt       >  2:3666162/1‑150 (MQ=255)
                                                                                                                                        tttctttcATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGAt       <  2:1085234/150‑1 (MQ=255)
                                                                                                                                          tctttcATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTc     >  1:2167910/1‑150 (MQ=255)
                                                                                                                                           ctttcATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCa    <  1:1375489/150‑1 (MQ=255)
                                                                                                                                            tttcATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCat   <  1:3799368/150‑1 (MQ=255)
                                                                                                                                             ttcATTTGATACCAGATAAAGAAAACGGTGGCCGGCCTTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGATTACATTTTGACCAACACTCCGCCATACAGCGCGGATTCata  >  2:2421353/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
CCATCAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATA  >  CP009273/2450189‑2450479

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCATCAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGC  >  CP009273/2450189‑2450483
                                                                                                                                                  |                                                                                                                                                    
CCATCAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGA                                                                                                                                                   >  SRR19973061.564379/1‑150 (MQ=60)
     AGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCA                                                                                                                                              <  SRR19973061.1614268/150‑1 (MQ=60)
     AGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCA                                                                                                                                              <  SRR19973061.234469/150‑1 (MQ=60)
        CGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGAT                                                                                                                                           >  SRR19973061.1708684/1‑150 (MQ=60)
         GAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATA                                                                                                                                          <  SRR19973061.188459/150‑1 (MQ=60)
         GAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATA                                                                                                                                          <  SRR19973061.188552/150‑1 (MQ=60)
          AGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAA                                                                                                                                         >  SRR19973061.2295592/1‑150 (MQ=60)
            ATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCAATTGATACCAGATAAAG                                                                                                                                       >  SRR19973061.273283/1‑150 (MQ=60)
                  CACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACG                                                                                                                                 <  SRR19973061.3242094/150‑1 (MQ=60)
                  CACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACG                                                                                                                                 <  SRR19973061.2869411/150‑1 (MQ=60)
                  CACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACG                                                                                                                                 >  SRR19973061.2270734/1‑150 (MQ=60)
                  CACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACG                                                                                                                                 >  SRR19973061.1119469/1‑150 (MQ=60)
                  CACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACG                                                                                                                                 >  SRR19973061.1760908/1‑150 (MQ=60)
                    CAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGT                                                                                                                               <  SRR19973061.1227155/150‑1 (MQ=60)
                     AACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTG                                                                                                                              <  SRR19973061.2334400/150‑1 (MQ=60)
                      ACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGG                                                                                                                             <  SRR19973061.3060551/150‑1 (MQ=60)
                      ACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGG                                                                                                                             >  SRR19973061.2192636/1‑150 (MQ=60)
                      ACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCCGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGG                                                                                                                             <  SRR19973061.3060512/150‑1 (MQ=60)
                      ACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCCGCGTCGCCGTGACGCATGATAAAAACTTGCAGATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGG                                                                                                                             <  SRR19973061.3060418/150‑1 (MQ=60)
                       CCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGC                                                                                                                            <  SRR19973061.805818/150‑1 (MQ=60)
                        CATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCC                                                                                                                           <  SRR19973061.1710945/150‑1 (MQ=60)
                            AGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGTGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCA                                                                                                                       >  SRR19973061.1088014/1‑150 (MQ=60)
                                GTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGT                                                                                                                   <  SRR19973061.1068509/150‑1 (MQ=60)
                                        ACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATC                                                                                                           <  SRR19973061.1289678/150‑1 (MQ=60)
                                         CGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCC                                                                                                          <  SRR19973061.2557140/150‑1 (MQ=60)
                                         CGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCC                                                                                                          <  SRR19973061.194584/150‑1 (MQ=60)
                                              GGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCA                                                                                                     >  SRR19973061.1391129/1‑150 (MQ=60)
                                               GAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCAC                                                                                                    >  SRR19973061.3713098/1‑150 (MQ=60)
                                               GAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCAC                                                                                                    <  SRR19973061.2938450/150‑1 (MQ=60)
                                                AATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACC                                                                                                   <  SRR19973061.3358308/150‑1 (MQ=60)
                                                 ATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCG                                                                                                  >  SRR19973061.2657836/1‑150 (MQ=60)
                                                    ACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAAT                                                                                               >  SRR19973061.2795144/1‑150 (MQ=60)
                                                        GCGGCATCGAGGGCTGCGTCGCAGTGACGCATGATAAAAACTTGCATTTTGCACCGATTTTGTTAACCAGATTCGCACGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAA                                                                                           <  SRR19973061.852377/150‑1 (MQ=60)
                                                          GGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACG                                                                                         <  SRR19973061.861797/150‑1 (MQ=60)
                                                            CATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCT                                                                                       <  SRR19973061.1061785/150‑1 (MQ=60)
                                                             ATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTG                                                                                      <  SRR19973061.565319/150‑1 (MQ=60)
                                                              TCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGT                                                                                     <  SRR19973061.1317761/150‑1 (MQ=60)
                                                              TCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGT                                                                                     <  SRR19973061.1000570/150‑1 (MQ=60)
                                                                 AGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTT                                                                                  <  SRR19973061.945163/150‑1 (MQ=60)
                                                                 AGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTT                                                                                  <  SRR19973061.2856895/150‑1 (MQ=60)
                                                                     CTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTAC                                                                              <  SRR19973061.33183/150‑1 (MQ=60)
                                                                     CTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTAC                                                                              >  SRR19973061.1442326/1‑150 (MQ=60)
                                                                           CGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAAT                                                                        <  SRR19973061.838394/150‑1 (MQ=60)
                                                                             CCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGG                                                                      <  SRR19973061.288307/150‑1 (MQ=60)
                                                                             CCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGG                                                                      <  SRR19973061.288212/150‑1 (MQ=60)
                                                                              CGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGC                                                                     <  SRR19973061.3786556/150‑1 (MQ=60)
                                                                               GTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCG                                                                    <  SRR19973061.2868600/150‑1 (MQ=60)
                                                                               GTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCG                                                                    <  SRR19973061.2867815/150‑1 (MQ=60)
                                                                               GTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCG                                                                    <  SRR19973061.2868649/150‑1 (MQ=60)
                                                                                 GACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTA                                                                  >  SRR19973061.3063148/1‑150 (MQ=60)
                                                                                 GACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTA                                                                  <  SRR19973061.2373662/150‑1 (MQ=60)
                                                                                 GACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTA                                                                  >  SRR19973061.1629245/1‑150 (MQ=60)
                                                                                 GACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTA                                                                  <  SRR19973061.3416234/150‑1 (MQ=60)
                                                                                     CATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTA                                                              <  SRR19973061.1353999/150‑1 (MQ=60)
                                                                                      ATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTAT                                                             >  SRR19973061.3599836/1‑150 (MQ=60)
                                                                                      ATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTAT                                                             >  SRR19973061.3379636/1‑150 (MQ=60)
                                                                                      ATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTAT                                                             >  SRR19973061.3584133/1‑150 (MQ=60)
                                                                                      ATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTAT                                                             >  SRR19973061.1477213/1‑150 (MQ=60)
                                                                                         ATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGT                                                          >  SRR19973061.910343/1‑150 (MQ=60)
                                                                                         ATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGT                                                          >  SRR19973061.105129/1‑150 (MQ=60)
                                                                                         ATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGT                                                          >  SRR19973061.846050/1‑150 (MQ=60)
                                                                                         ATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGT                                                          >  SRR19973061.3345920/1‑150 (MQ=60)
                                                                                         ATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGT                                                          >  SRR19973061.845454/1‑150 (MQ=60)
                                                                                         ATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGT                                                          <  SRR19973061.3441137/150‑1 (MQ=60)
                                                                                         ATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGT                                                          >  SRR19973061.2021019/1‑150 (MQ=60)
                                                                                         ATAAAAACTTGCATAGTGCACCGCTTTTGTGAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGT                                                          <  SRR19973061.3277690/150‑1 (MQ=60)
                                                                                          TAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTTGCTCAATGGCGGAAGGATAGTA                                                         >  SRR19973061.3550846/1‑150 (MQ=60)
                                                                                          TAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTC                                                         >  SRR19973061.627738/1‑150 (MQ=60)
                                                                                          TAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTC                                                         >  SRR19973061.627758/1‑150 (MQ=60)
                                                                                            AAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAA                                                       >  SRR19973061.2250682/1‑150 (MQ=60)
                                                                                            AAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAA                                                       >  SRR19973061.2251234/1‑150 (MQ=60)
                                                                                               ACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATAT                                                    <  SRR19973061.3615606/150‑1 (MQ=60)
                                                                                               ACTTGCATAGTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATAT                                                    <  SRR19973061.373368/150‑1 (MQ=60)
                                                                                                 TTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGC                                                  <  SRR19973061.2758481/150‑1 (MQ=60)
                                                                                                  TGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCG                                                 <  SRR19973061.149964/150‑1 (MQ=60)
                                                                                                  TGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCG                                                 <  SRR19973061.2066975/150‑1 (MQ=60)
                                                                                                  TGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCG                                                 >  SRR19973061.775981/1‑150 (MQ=60)
                                                                                                  TGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCG                                                 <  SRR19973061.148027/150‑1 (MQ=60)
                                                                                                  TGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCG                                                 <  SRR19973061.148019/150‑1 (MQ=60)
                                                                                                   GCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGT                                                >  SRR19973061.2308718/1‑150 (MQ=60)
                                                                                                    CATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTT                                               >  SRR19973061.1845982/1‑150 (MQ=60)
                                                                                                       ATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTAC                                            >  SRR19973061.2932725/1‑150 (MQ=60)
                                                                                                        TTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATATCAGATAAAGAAAACGGTGGCCGTCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACA                                           <  SRR19973061.1655677/150‑1 (MQ=60)
                                                                                                         TGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACAT                                          <  SRR19973061.3394880/150‑1 (MQ=60)
                                                                                                             CCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTG                                      <  SRR19973061.3419508/150‑1 (MQ=60)
                                                                                                             CCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTG                                      <  SRR19973061.2231574/150‑1 (MQ=60)
                                                                                                               GCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGAC                                    >  SRR19973061.1453212/1‑150 (MQ=60)
                                                                                                               GCTTTTGTTAACCAGATTCGCCCGTTATCTTTCATTTGATAACAGATAAAGAAAACGGTGGCCGGCATAGTGCCTTATCCATTCAACGAATGAAACGCAGTTTTTTACCTCAATGGCGTAAAAATAGTCAATATGCGTTTACATTTTGAC                                    >  SRR19973061.1683952/1‑150 (MQ=60)
                                                                                                                CTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACC                                   >  SRR19973061.3260748/1‑150 (MQ=60)
                                                                                                                 TTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCA                                  <  SRR19973061.3274975/150‑1 (MQ=60)
                                                                                                                   TTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAAC                                >  SRR19973061.578425/1‑150 (MQ=60)
                                                                                                                   TTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAAC                                <  SRR19973061.530703/150‑1 (MQ=60)
                                                                                                                   TTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAAC                                <  SRR19973061.2442282/150‑1 (MQ=60)
                                                                                                                    TGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACA                               >  SRR19973061.2946142/1‑150 (MQ=60)
                                                                                                                    TGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACA                               >  SRR19973061.52062/1‑150 (MQ=60)
                                                                                                                    TGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACA                               >  SRR19973061.50846/1‑150 (MQ=60)
                                                                                                                    TGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACA                               >  SRR19973061.2293245/1‑150 (MQ=60)
                                                                                                                    TGTTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATAGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACA                               >  SRR19973061.2293121/1‑150 (MQ=60)
                                                                                                                      TTAACCAGATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACT                             <  SRR19973061.3186542/150‑1 (MQ=60)
                                                                                                                             GATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCAT                      >  SRR19973061.3465331/1‑150 (MQ=60)
                                                                                                                             GATTCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACATCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCAT                      >  SRR19973061.3466453/1‑150 (MQ=60)
                                                                                                                                TCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCA                   >  SRR19973061.1174002/1‑150 (MQ=60)
                                                                                                                                TCGCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCA                   >  SRR19973061.2094846/1‑150 (MQ=60)
                                                                                                                                  GCCCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGC                 >  SRR19973061.97233/1‑150 (MQ=60)
                                                                                                                                    CCGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGC               <  SRR19973061.541403/150‑1 (MQ=60)
                                                                                                                                     CGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGTCATTGTGCCTTATCCATACACCGAATGAAACTCTGAATTTTACCTCAATTGCGTAAGAATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCAATTCATCGCG              >  SRR19973061.514982/1‑150 (MQ=60)
                                                                                                                                     CGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCG              <  SRR19973061.2430697/150‑1 (MQ=60)
                                                                                                                                     CGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCG              >  SRR19973061.515003/1‑150 (MQ=60)
                                                                                                                                     CGTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCG              <  SRR19973061.1063311/150‑1 (MQ=60)
                                                                                                                                      GTTTTCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGG             >  SRR19973061.2592975/1‑150 (MQ=60)
                                                                                                                                          TCTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTC         >  SRR19973061.2173351/1‑150 (MQ=60)
                                                                                                                                           CTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCA        <  SRR19973061.325800/150‑1 (MQ=60)
                                                                                                                                           CTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCA        <  SRR19973061.1378964/150‑1 (MQ=60)
                                                                                                                                           CTTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTGACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCA        <  SRR19973061.3522554/150‑1 (MQ=60)
                                                                                                                                            TTTCATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCAT       <  SRR19973061.3809053/150‑1 (MQ=60)
                                                                                                                                                ATTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAG   >  SRR19973061.1275321/1‑150 (MQ=60)
                                                                                                                                                 TTTGATACCAGATAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGC  <  SRR19973061.83525/150‑1 (MQ=60)
                                                                                                                                                  |                                                                                                                                                    
CCATCAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGAGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGC  >  CP009273/2450189‑2450483

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑