Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F181 I0 R1 1262 323.1 10297870 97.5% 10040423 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,215,927 C→A 17.9% intergenic (+119/‑65) ebgR → / → ebgA transcriptional repressor/evolved beta‑D‑galactosidase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,215,9270CA17.9% 911.2 / 127.0 318intergenic (+119/‑65)ebgR/ebgAtranscriptional repressor/evolved beta‑D‑galactosidase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (142/117);  new base A (39/20);  total (181/137)
Fisher's exact test for biased strand distribution p-value = 1.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 329 total aligned reads displayed.

AAATTAAAACTGCGCGGCACGACCCGTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGC  >  CP009273/3215779‑3216076
                                                                                                                                                    |                                                                                                                                                     
 aaTTAAAACTGCGCGGCACGACCCGTTAAATCCCCTTACACACTGTCCGGCAATCGGTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTccc                                                                                                                                                     >  2:4192674/1‑150 (MQ=255)
  aTTAAAACTGCGCGGCACGACCCGTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTcccc                                                                                                                                                    <  2:3516965/150‑1 (MQ=255)
   ttAAAACTGCGCGGCACGACCCGTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCa                                                                                                                                                   <  2:2156137/150‑1 (MQ=255)
        aCTGCGCGGCACGACCCGTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATTCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAAATCCCCAGCAAt                                                                                                                                              >  2:5016570/1‑150 (MQ=255)
        aCTGCGCGGCACGACCCGTTAAATCCCCTGACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAAt                                                                                                                                              <  2:3587278/150‑1 (MQ=255)
           gcgcGGCACGACCCGTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTac                                                                                                                                           >  2:3852959/1‑150 (MQ=255)
            cgcgGCACGACCCGTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTACCCAGCAATTaca                                                                                                                                          <  2:623500/150‑1 (MQ=255)
               ggCACGACCCGTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTTCTAAACTCCCCAGCAATTACACaa                                                                                                                                       >  1:201959/1‑150 (MQ=255)
                  aCGACCCGTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACt                                                                                                                                    <  1:4535102/150‑1 (MQ=255)
                    gACCCGTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTCCTTTTTATTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTAc                                                                                                                                  >  2:3154083/1‑150 (MQ=255)
                       ccGTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTAccct                                                                                                                               >  2:3127768/1‑148 (MQ=255)
                       ccGTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCAt                                                                                                                               <  1:3015053/150‑1 (MQ=255)
                        cGTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATc                                                                                                                              <  2:2849742/150‑1 (MQ=255)
                        cGTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTACCCAGCAATTACACAAACTACCATc                                                                                                                              >  2:4889917/1‑150 (MQ=255)
                        cGTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTACCCAGCAATTACACAAACTACCATc                                                                                                                              <  1:4483109/150‑1 (MQ=255)
                             aaTCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCAt                                                                                                                         <  2:2252350/150‑1 (MQ=255)
                                ccccTTACACACTGTCCGGCACTCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTACCCAGCAATTACACAAACTACCATCACCATGaa                                                                                                                      <  2:1986486/150‑1 (MQ=255)
                                 cccTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAAt                                                                                                                     <  1:3213941/150‑1 (MQ=255)
                                   cTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACGTTTTAGTAAAAATTTTACTAAACTCCCCAGCACTTACACAAACTACCATCACCATGAATgg                                                                                                                   >  1:819103/1‑150 (MQ=255)
                                     tACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTATTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATTGtt                                                                                                                 >  1:4656479/1‑150 (MQ=255)
                                     tACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGtt                                                                                                                 <  1:3810374/150‑1 (MQ=255)
                                       cacacTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTcc                                                                                                               <  2:4486607/150‑1 (MQ=255)
                                       cacacTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTAACATCACCATGAATGGTTcc                                                                                                               >  2:2455725/1‑150 (MQ=255)
                                         cacTGTCCGGCACTCGTTTTTGCCGGACAGTGCTGCCGGTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACGTTTTAGGAAAAATTTTACTAAACTCCCCAGCACTTACACAAACAACCATCACCATGCATGGGTCCGa                                                                                                             >  2:1648905/1‑150 (MQ=255)
                                           cTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGAtt                                                                                                           <  2:4297760/150‑1 (MQ=255)
                                             gtacGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGTTCCAGTTAAAGTAAATGCAATTAACTGCTAAATTTTATTAAAAATTTTAATAAACAACCCAGCAATTAAACAAAAAAACAACACAAAGAAATGTTACGATTtc                                                                                                         >  2:2780488/4‑150 (MQ=255)
                                             gTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTACCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTtc                                                                                                         <  2:1050784/150‑1 (MQ=255)
                                               ccGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTTTTGCAGTTAAAGTAAATTGATTTAACTGCGACATTTTAGTAAAAAATTTACAAAAAAACCCAGCAAATACACAAACAAACAAAACCAAGAATTGTTACGATTtctc                                                                                                       >  1:937719/1‑150 (MQ=255)
                                                cGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTtctct                                                                                                      <  1:2347415/150‑1 (MQ=255)
                                                   cAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTAGTGATCCAGTTAAAGTAAAAGCATTTACCTGCTACTTTTTAGTAAAAATTTTAATAAAATACCCAGCAATTACACAAACTACCATCACCAAGAAAGGTTCCGATTTCTCTCTa                                                                                                   >  1:2843552/1‑150 (MQ=255)
                                                     aTCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTAcc                                                                                                 <  1:3364526/150‑1 (MQ=255)
                                                         tttttGCCGGACAGTGCTGCCGTTTATTTTGGTGATGCAGTTAAAGTAAATTCATTTAACATGTACTTTTTATTAAAAATTTTAATAAACTACCCAGCAATTACACAAAATACCAACACCAAGAATGGTTGCGAGTTATCTATAACGGGa                                                                                             >  1:578145/1‑150 (MQ=255)
                                                         tttttGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGa                                                                                             <  2:2721957/150‑1 (MQ=255)
                                                              gCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGccc                                                                                        >  2:2983263/1‑150 (MQ=255)
                                                              gCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACAAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGccc                                                                                        >  2:1272757/1‑150 (MQ=255)
                                                                        gctgcCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGc                                                                              >  2:4994648/1‑150 (MQ=255)
                                                                         ctgcCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCt                                                                             <  1:1084769/150‑1 (MQ=255)
                                                                          tgcCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTg                                                                            >  1:775943/1‑150 (MQ=255)
                                                                          tgcCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTg                                                                            <  1:1916377/150‑1 (MQ=255)
                                                                              gTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGaa                                                                        <  1:605785/150‑1 (MQ=255)
                                                                               tttATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGATACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATAGCTGGGaaa                                                                       >  2:2106950/1‑150 (MQ=255)
                                                                                    tttCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCAAGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACAtt                                                                  >  2:4585053/1‑150 (MQ=255)
                                                                                     ttCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTc                                                                 >  1:4369068/1‑150 (MQ=255)
                                                                                      tCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCa                                                                >  1:1673224/1‑150 (MQ=255)
                                                                                       cGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAg                                                               >  2:1371005/1‑150 (MQ=255)
                                                                                       cGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTACCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAg                                                               >  1:200668/1‑150 (MQ=255)
                                                                                            tCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACCTTCAGCTCAc                                                          >  1:2782564/1‑150 (MQ=255)
                                                                                            tCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCAc                                                          <  1:4174122/150‑1 (MQ=255)
                                                                                            tCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCAc                                                          >  1:1612138/1‑150 (MQ=255)
                                                                                              cAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCAccc                                                        <  1:1349976/150‑1 (MQ=255)
                                                                                                gTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCAc                                                      >  2:346238/1‑150 (MQ=255)
                                                                                                gTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCAc                                                      <  2:3775170/150‑1 (MQ=255)
                                                                                                gTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACAGTCAGCTCACCCAc                                                      >  1:3394990/1‑150 (MQ=255)
                                                                                                  tAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGa                                                    >  1:4250027/1‑150 (MQ=255)
                                                                                                  tAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGa                                                    <  1:3971145/150‑1 (MQ=255)
                                                                                                  naaaGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGa                                                    >  1:4045965/2‑150 (MQ=255)
                                                                                                   aaaGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGaa                                                   <  1:1206060/150‑1 (MQ=255)
                                                                                                      gTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAAc                                                <  1:1158154/150‑1 (MQ=255)
                                                                                                       tAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAAcc                                               <  2:4520376/150‑1 (MQ=255)
                                                                                                       tAAATGCATTTACCTGCGACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAAcc                                               <  2:4084675/150‑1 (MQ=255)
                                                                                                        aaaTGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCg                                              >  1:317128/1‑150 (MQ=255)
                                                                                                        aaaTGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTACCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCg                                              <  2:1556128/150‑1 (MQ=255)
                                                                                                         aaTGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGa                                             >  2:4435774/1‑150 (MQ=255)
                                                                                                            gCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACtt                                          >  1:681016/1‑150 (MQ=255)
                                                                                                            gCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACtt                                          >  1:4396281/1‑150 (MQ=255)
                                                                                                            gCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTACCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACtt                                          >  1:5100022/1‑150 (MQ=255)
                                                                                                               tttACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTgcg                                       <  2:3285443/150‑1 (MQ=255)
                                                                                                               tttACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTgcg                                       >  2:1912110/1‑150 (MQ=255)
                                                                                                                ttACCTGCTACTTTTTAGTAAAAATTTTACTAAACTACCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTgcgc                                      >  2:3638979/1‑150 (MQ=255)
                                                                                                                  aCCTGCTACTTTTTAGTAAAAATTTTACTAAACTACCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGcgccg                                    >  2:4982650/1‑150 (MQ=255)
                                                                                                                     tGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCgcgt                                 >  2:2513549/1‑150 (MQ=255)
                                                                                                                      gCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCgcgtg                                <  2:2502363/150‑1 (MQ=255)
                                                                                                                       cTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCgcgtgc                               <  1:2137943/150‑1 (MQ=255)
                                                                                                                       cTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCgcgtgc                               <  2:4015072/150‑1 (MQ=255)
                                                                                                                       cTACTTTTTAGTAAAAATTTTACTAAACTACCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCgcgtgc                               >  2:1136601/1‑150 (MQ=255)
                                                                                                                        tACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAAGTACACAAACTACCCTCACCAGGAATGGTTCCGATGTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCCCGAAAACCGACTTGCGCCgcgtgcg                              <  2:3666832/150‑1 (MQ=255)
                                                                                                                         aCTTTTTAGTAAAAATTTTACTAAACTACCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCgcgtgcgt                             >  2:844825/1‑150 (MQ=255)
                                                                                                                           tttttAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGACTGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTAc                           <  2:3743695/150‑1 (MQ=255)
                                                                                                                           tttttAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTAc                           >  1:2321905/1‑150 (MQ=255)
                                                                                                                            ttttAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTCTGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACt                          >  2:2611093/1‑150 (MQ=255)
                                                                                                                            ttttAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACt                          >  1:4884553/1‑150 (MQ=255)
                                                                                                                             tttAGTAAAAATTTTACTAAACTCCCCAGCAATAACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACtt                         >  1:2556989/1‑150 (MQ=255)
                                                                                                                              ttAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACttt                        <  1:3127796/150‑1 (MQ=255)
                                                                                                                                   aaaaaTTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTc                   <  2:1808157/150‑1 (MQ=255)
                                                                                                                                     aaaTTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCat                 <  2:4097452/150‑1 (MQ=255)
                                                                                                                                         tttACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGa             >  2:593153/1‑150 (MQ=255)
                                                                                                                                          ttACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGAt            >  1:3620800/1‑150 (MQ=255)
                                                                                                                                           tACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGAtt           >  1:1721836/1‑150 (MQ=255)
                                                                                                                                            aCTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTc          >  2:2389744/1‑150 (MQ=255)
                                                                                                                                              tAAACTACCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTg        >  2:3107276/1‑150 (MQ=255)
                                                                                                                                               aaaCTCCCCAGCATTTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGt       <  2:5148158/150‑1 (MQ=255)
                                                                                                                                               aaaCTACCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGt       >  2:572934/1‑150 (MQ=255)
                                                                                                                                               aaaCTACCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGt       >  2:2681641/1‑150 (MQ=255)
                                                                                                                                                aaCTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGTTTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGtt      <  2:4696817/150‑1 (MQ=255)
                                                                                                                                                aaCTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGtt      <  2:475324/150‑1 (MQ=255)
                                                                                                                                                 aCTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTg     <  2:1712828/150‑1 (MQ=255)
                                                                                                                                                  cTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTgc    >  2:4377625/1‑150 (MQ=255)
                                                                                                                                                    ccccAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTgcgc  >  2:4970571/1‑150 (MQ=255)
                                                                                                                                                    ccccAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTgcgc  >  1:3587143/1‑150 (MQ=255)
                                                                                                                                                    ccccAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTgcgc  >  2:889887/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
AAATTAAAACTGCGCGGCACGACCCGTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGC  >  CP009273/3215779‑3216076

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A