Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F142 I0 R1 859 274.6 8723680 96.4% 8409627 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,215,927 C→A 22.9% intergenic (+119/‑65) ebgR → / → ebgA transcriptional repressor/evolved beta‑D‑galactosidase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,215,9270CA22.9% 523.1 / 119.3 223intergenic (+119/‑65)ebgR/ebgAtranscriptional repressor/evolved beta‑D‑galactosidase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (82/88);  new base A (27/26);  total (109/114)
Fisher's exact test for biased strand distribution p-value = 7.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 229 total aligned reads displayed.

CAAATTAAAACTGCGCGGCACGACCCGTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCG  >  CP009273/3215778‑3216075
                                                                                                                                                     |                                                                                                                                                    
 aaaTTAAAACTGCGCGGCACGACCCGTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTcc                                                                                                                                                     >  2:3946255/1‑150 (MQ=255)
  aaTTAAAACTGCGCGGCACGACCCGTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTccc                                                                                                                                                    <  1:213023/150‑1 (MQ=255)
    ttAAAACTGCGCGGCACGACCCGTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCa                                                                                                                                                  <  2:753583/150‑1 (MQ=255)
    ttAAAACTGCGCGGCACGACCCGTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCa                                                                                                                                                  <  1:3840205/150‑1 (MQ=255)
       aaaCTGCGCGGCACGACCCGTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCa                                                                                                                                               <  1:3669149/150‑1 (MQ=255)
        aaCTGCGCGGCACGACCCGTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCaa                                                                                                                                              <  1:2717655/150‑1 (MQ=255)
         aCTGCGCGGCACGACCCGTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTACCCAGCAAt                                                                                                                                             <  1:1603606/150‑1 (MQ=255)
         aCTGCGCGGCACGACCCGTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTACCCAGCAAt                                                                                                                                             <  1:4251543/150‑1 (MQ=255)
          cTGCGCGGCACGACCCGTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTTCCTGCTACTTTTTAGTAAAAATTTTACTAAAATCCCCCGCAAtt                                                                                                                                            >  2:576983/1‑150 (MQ=255)
             cgcgGCACGACCCGTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTGTATTTTAGTGATACACTTAAAGTAAATTCAGTTAAAAGCGACATTTTATTAAAAATTTTAAAAAAATAACCACCAATTaaa                                                                                                                                         >  1:504213/1‑148 (MQ=255)
                 gCACGACCCGTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACaaa                                                                                                                                     <  1:3817391/150‑1 (MQ=255)
                    cGACCCGTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTTGGGTTGCAGTTAAAGTAAATTGATTTTCCTGCTAAATTTTATTAAAAAATTTTATAAAAAACCCACCCATTACAaaaaaaa                                                                                                                                  >  1:572050/1‑147 (MQ=255)
                    cGACCCGTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTa                                                                                                                                  <  1:2879167/150‑1 (MQ=255)
                    cGACCCGTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTAACTGCTACTTTTTAGTAAAAATTTTACAAAACTCCCCAGCCATTACACAAAcga                                                                                                                                  >  2:3151782/1‑148 (MQ=255)
                    cGACCCGTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTAACAGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTa                                                                                                                                  >  2:314669/1‑150 (MQ=255)
                     gACCCGTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTGTATTTTAGTGATCCAGTTAAATTAAATGCATTTACCTGCGACGTTTTATTAAAAAATTTACTAAACAACCCAGCAAATAAACCAAcaaa                                                                                                                                 >  1:2799886/1‑147 (MQ=255)
                        ccGTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTACCCAGCAATTACACAAACTACCAt                                                                                                                              <  2:936717/150‑1 (MQ=255)
                         cGTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATc                                                                                                                             <  2:1541762/150‑1 (MQ=255)
                            tAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCAcc                                                                                                                          <  2:2227289/150‑1 (MQ=255)
                                tCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTGTATTTTCGTGGTCCAGTTAAAGTAAATGCATTTACCGGCTACGTTTTAGTTAAAATTTTTCTAAACTCCCCAGCAAGTACACAAACTACCATCACCATGa                                                                                                                      >  2:3509968/1‑150 (MQ=255)
                                 ccccTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTACCCAGCAATTACACAAACTACCATCACCATGaa                                                                                                                     <  1:3182733/150‑1 (MQ=255)
                                        cacacTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTcc                                                                                                              <  1:2865602/150‑1 (MQ=255)
                                        cacacTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTcc                                                                                                              <  1:3567524/150‑1 (MQ=255)
                                        cacacTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTAGTGATCCAGTTAAATTAAATGCATTTTACTGCTACTTTTTATTAAAAATTTTACTAAAATCCCCAGCAATTACACAAAATACCATCCCCAAGAATGGTTcc                                                                                                              >  2:859408/1‑150 (MQ=255)
                                        cacacTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTAGTGATCCAGTTAAAGTAAATGCATTTACCTGCGACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTAACATCACCATGAATGGTTcc                                                                                                              >  2:2487841/1‑150 (MQ=255)
                                           acTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGCTCCAGTTAAAGTAAATGCATTTACCTGCGACTTTTTAGTAAAAATTTTACTAAACTACCCCGCACTTACACAAACAACCAACACCCAGAATGGTTCCGAt                                                                                                           >  2:2033721/1‑150 (MQ=255)
                                           acTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAATTAAATGCCTTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCCATTACACAAACTAACATAACCATGAATGGTTCCGAt                                                                                                           >  2:2437398/1‑150 (MQ=255)
                                           acTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGAt                                                                                                           <  2:3227463/150‑1 (MQ=255)
                                           acTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTACCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGAt                                                                                                           <  2:738083/150‑1 (MQ=255)
                                           acTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTACCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGAt                                                                                                           <  2:739358/150‑1 (MQ=255)
                                             tGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTAGTGGTACAGTTAAATTAAATGAATTTTACTGCTAAATTTTTTTTAAAATTTTTCTAAACTAACCAGCAATTAAACAAAATTACAACACCAATAATGtttgggcttt                                                                                                         >  2:3287805/1‑143 (MQ=255)
                                                 cGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCCTCACCATGAATGGTTCCGATTtctct                                                                                                     <  2:1475068/150‑1 (MQ=255)
                                                    cAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTa                                                                                                  <  2:1709079/150‑1 (MQ=255)
                                                           ttttGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAg                                                                                           <  2:621698/150‑1 (MQ=255)
                                                             ttGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAAAGCATTTAACTTCTACTTTTTATTAAAAATTTTACTAAACAACCCAGCAATTACACAAACAACCATCAACATGAAATGTTCCGATTTCTCTCTACCGGGAGGc                                                                                         >  2:2390170/1‑150 (MQ=255)
                                                                 cGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTa                                                                                     <  2:728401/150‑1 (MQ=255)
                                                                     cAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGaa                                                                                 <  2:1329591/150‑1 (MQ=255)
                                                                      aGTGCTGCCGTTTATTTTCGTGTTCCACTTAAAGTAAATTCAATTAACTGCGACGTTTTAGTAAAAAATTTACTAAAAAACCCACCCAATACACAAACAACCAACACCAAGAATGGGTCCGCTTTCTCTCTACCCGGCGGCCCTAAGaaa                                                                                >  2:3820956/1‑149 (MQ=255)
                                                                      aGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATTCATTTAACTGCAAAATTTTAGTAAAAATTTTAAAAAACAACCCAGCAATTAAACAAAAAAACAACACCAAGTATGGTTCCGATTTCTATAGAACGCGAGGCCCTATGAAt                                                                                >  2:560283/1‑150 (MQ=255)
                                                                       gTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTACCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATc                                                                               <  1:2907791/150‑1 (MQ=255)
                                                                        tgctgcCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCg                                                                              <  1:1866054/150‑1 (MQ=255)
                                                                           tgcCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTg                                                                           >  1:2088524/1‑150 (MQ=255)
                                                                           tgcCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTACCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTg                                                                           <  1:2349984/150‑1 (MQ=255)
                                                                              cGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGa                                                                        <  1:3938137/150‑1 (MQ=255)
                                                                              cGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGa                                                                        >  1:351882/1‑150 (MQ=255)
                                                                                 ttATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTACCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGaaaa                                                                     >  2:1503698/1‑150 (MQ=255)
                                                                                  tATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAAc                                                                    <  2:2430290/150‑1 (MQ=255)
                                                                                  tATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAAc                                                                    <  1:3110680/150‑1 (MQ=255)
                                                                                  tATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTACCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAAc                                                                    >  1:4201189/1‑150 (MQ=255)
                                                                                    ttttCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACAt                                                                  <  1:2485523/150‑1 (MQ=255)
                                                                                    ttttCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACAt                                                                  <  1:308619/150‑1 (MQ=255)
                                                                                         gTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGc                                                             >  1:4358523/1‑150 (MQ=255)
                                                                                          tGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTACCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCt                                                            <  2:2555964/150‑1 (MQ=255)
                                                                                           gATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTACCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTc                                                           >  1:2739106/1‑150 (MQ=255)
                                                                                              ccAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCAcc                                                        >  2:1863213/1‑150 (MQ=255)
                                                                                              ccAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCAcc                                                        <  1:3982697/150‑1 (MQ=255)
                                                                                                aGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCa                                                      >  2:3433531/1‑150 (MQ=255)
                                                                                                   tAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGa                                                   <  1:797063/150‑1 (MQ=255)
                                                                                                   tAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGa                                                   >  2:1113506/1‑150 (MQ=255)
                                                                                                   tAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGa                                                   <  1:795769/150‑1 (MQ=255)
                                                                                                     aaGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTACCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAATACATTAAGCTCACCCACGaaa                                                 >  1:1392172/1‑150 (MQ=255)
                                                                                                      aGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGaaaa                                                >  2:297363/1‑150 (MQ=255)
                                                                                                            tGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACt                                          <  1:4263822/150‑1 (MQ=255)
                                                                                                             gCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACtt                                         <  2:1961646/150‑1 (MQ=255)
                                                                                                             gCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACtt                                         <  1:3093946/150‑1 (MQ=255)
                                                                                                                 tgaCCTGCTACTTTGTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACCAACTACCATCACCAGGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTgcgc                                     <  2:4066365/148‑1 (MQ=255)
                                                                                                                  tACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGcgcc                                    >  2:3133977/1‑150 (MQ=255)
                                                                                                                    ccTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGcgccgc                                  >  1:1927663/1‑150 (MQ=255)
                                                                                                                      tGCTACTTTTTAGTAAAAATTTTACTAAACTACCCAGCAATTACACAAACTACCATCACCATGAATGTAACCGATTTCTCTCTACCGGGAGGACCTATCAATAGATGGGAAAACATACAGCTCACCCAAGAAAACCGACTTTCTCAgcgt                                >  1:1433539/1‑150 (MQ=255)
                                                                                                                        cTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCgcgggc                              >  2:2686848/1‑150 (MQ=255)
                                                                                                                        cTACTTTTTAGTAAAAATTTTACTAAACTACCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCgcgtgc                              >  2:2203898/1‑150 (MQ=255)
                                                                                                                         tACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCgcgtgcg                             <  2:4102818/150‑1 (MQ=255)
                                                                                                                         tACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCgcgtgcg                             >  1:347860/1‑150 (MQ=255)
                                                                                                                         tACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCgcgtgcg                             >  1:347066/1‑150 (MQ=255)
                                                                                                                         tACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCgcgtgcg                             >  2:1626623/1‑150 (MQ=255)
                                                                                                                         tACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCgcgtgcg                             >  2:1626144/1‑150 (MQ=255)
                                                                                                                          aCTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCgcgtgcgt                            >  2:3441430/1‑150 (MQ=255)
                                                                                                                          aCTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCgcgtgcgt                            >  2:2824254/1‑150 (MQ=255)
                                                                                                                           cTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTa                           >  2:3768428/1‑150 (MQ=255)
                                                                                                                             ttttAGTAAAAATTTTACTAAACTACCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACt                         <  2:92345/150‑1 (MQ=255)
                                                                                                                                tAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACtttt                      >  1:4041412/1‑150 (MQ=255)
                                                                                                                                 aGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACttttt                     >  1:1844327/1‑150 (MQ=255)
                                                                                                                                 aGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATACAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACttttt                     >  1:1699588/1‑150 (MQ=255)
                                                                                                                                   tAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCCCGAAAACCGACTTGCGCCGCGTGCGTACttttttt                   <  1:1122103/150‑1 (MQ=255)
                                                                                                                                     aaaaTTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCa                 >  1:685044/1‑150 (MQ=255)
                                                                                                                                      aaaTTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCat                <  1:4104030/150‑1 (MQ=255)
                                                                                                                                      aaaTTTTACTAAACTACCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCat                <  1:4293476/150‑1 (MQ=255)
                                                                                                                                       aaTTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCata               >  1:632519/1‑150 (MQ=255)
                                                                                                                                       aaTTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCata               >  1:917709/1‑150 (MQ=255)
                                                                                                                                         ttttACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATg             >  2:469784/1‑150 (MQ=255)
                                                                                                                                         ttttACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATg             <  1:4169643/150‑1 (MQ=255)
                                                                                                                                               tAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTg       >  1:2966078/1‑150 (MQ=255)
                                                                                                                                               tAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTg       >  1:1098262/1‑150 (MQ=255)
                                                                                                                                                aaaCTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGt      <  2:504213/150‑1 (MQ=255)
                                                                                                                                                aaaCTACCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGt      >  1:1542330/1‑150 (MQ=255)
                                                                                                                                                  aCTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTg    >  2:3911026/1‑150 (MQ=255)
                                                                                                                                                  aCTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTg    >  2:3909652/1‑150 (MQ=255)
                                                                                                                                                  aCTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTg    >  1:3359685/1‑150 (MQ=255)
                                                                                                                                                  aCTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGAATTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGttt    >  1:3359688/1‑149 (MQ=255)
                                                                                                                                                    tCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTgcg  >  1:1564629/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CAAATTAAAACTGCGCGGCACGACCCGTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCG  >  CP009273/3215778‑3216075

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A