Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F202 I0 R1 1397 275.7 9089454 95.2% 8653160 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,215,927 C→A 10.5% intergenic (+119/‑65) ebgR → / → ebgA transcriptional repressor/evolved beta‑D‑galactosidase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,215,9270CA10.5% 761.1 / 46.3 230intergenic (+119/‑65)ebgR/ebgAtranscriptional repressor/evolved beta‑D‑galactosidase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (107/98);  new base A (15/10);  total (122/108)
Fisher's exact test for biased strand distribution p-value = 5.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 240 total aligned reads displayed.

CAAATTAAAACTGCGCGGCACGACCCGTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAA‑TTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGC  >  CP009273/3215778‑3216076
                                                                                                                                                      |                                                                                                                                                     
cAAATTAAAACTGCGCGGCACGACCCGTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAA‑TTTTACTAAACTc                                                                                                                                                       >  2:3783543/1‑150 (MQ=255)
     tAAAACTGCGCGGCACGACCCGTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAA‑TTTTACTAAACTCCCCAg                                                                                                                                                  <  1:3022569/150‑1 (MQ=255)
      aaaaCTGCGCGGCACGACCCGTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAA‑TTTTACTAAACTCCCCAGc                                                                                                                                                 >  1:3401443/1‑150 (MQ=255)
         aCTGCGCGGCACGACCCGTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAA‑TTTTACTAAACTCCCCAGCAAt                                                                                                                                              <  2:1475820/150‑1 (MQ=255)
         aCTGCGCGGCACGACCCGTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAA‑TTTTACTAAACTCCCCAGCAAt                                                                                                                                              <  1:715513/150‑1 (MQ=255)
          cTGCGCGGCACGACCCGTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAA‑TTTTACTAAACTCCCCAGCAAtt                                                                                                                                             <  1:3479488/150‑1 (MQ=255)
          cTGCGCGGCACGACCCGTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAA‑TTTTACTAAACTCCCCAGCAAtt                                                                                                                                             <  2:2160755/150‑1 (MQ=255)
          cTGCGCGGCACGACCCGTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAA‑TTTTACTAAACTCCCCAGCAAtt                                                                                                                                             <  1:2726743/150‑1 (MQ=255)
          cTGCGCGGCACGACCCGTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAA‑TTTTACTAAACTCCCCAGCAAtt                                                                                                                                             <  1:1206558/150‑1 (MQ=255)
          cTGCGCGGCACGACCCGTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGATGCCGATTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAA‑TTTTACTAAACTCCCCAGCAAtt                                                                                                                                             <  2:4399342/150‑1 (MQ=255)
             cgcgGCACGACCCGTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAA‑TTTTACTAAACTACCCAGCAATTaca                                                                                                                                          <  2:3487348/150‑1 (MQ=255)
              gcgGCACGACCCGTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAA‑TTTTACTAAACTCCCCAGCAATTacac                                                                                                                                         <  1:4087037/150‑1 (MQ=255)
               cgGCACGACCCGTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAA‑TTTTACTAAACTCCCCAGCAATTAcaca                                                                                                                                        <  2:344488/150‑1 (MQ=255)
                 gCACGACCCGTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAA‑TTTTACTAAACTCCCCAGCAATTACACaaa                                                                                                                                      <  1:475279/150‑1 (MQ=255)
                    cGACCCGTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTATTAAAAA‑TTTTACTAAACTCCCCAGCAAATACACAAACTa                                                                                                                                   >  1:4346262/1‑150 (MQ=255)
                    cGACCCGTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAA‑TTTTACTAAACTCCCCAGCAATTACACAAACTa                                                                                                                                   >  1:1912716/1‑150 (MQ=255)
                      aCCCGTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAA‑TTTTACTAAACTCCCCAGCAATTACACCAACTAcc                                                                                                                                 >  2:306052/1‑150 (MQ=255)
                       cccGTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAA‑TTTTACTAAACTCCCCAGCAATTACACAAACTACCa                                                                                                                                <  1:2687290/150‑1 (MQ=255)
                       cccGTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAA‑TTTTACTAAACTCCCCAGCAATTACACAAACTACCa                                                                                                                                <  1:1670605/150‑1 (MQ=255)
                       cccGTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAA‑TTTTACTAAACTCCCCAGCAATTACACAAACTACCa                                                                                                                                <  1:1670582/150‑1 (MQ=255)
                         cGTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAA‑TTTTACTAAACTCCCCAGCAATTACACAAACTACCATc                                                                                                                              <  2:186028/150‑1 (MQ=255)
                         cGTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCAAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAA‑TTTTACTAAACTCCCCAGCAATTACACAAACTACCATc                                                                                                                              <  1:4450355/150‑1 (MQ=255)
                          gTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAA‑TTTTACTAAAATCCCCAGCAATTACACAAACTACCAACa                                                                                                                             >  1:93470/1‑150 (MQ=255)
                            tAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAA‑TTTTACTAAACTCCCCAGCAATTACACAAACTACCATCAcc                                                                                                                           <  2:1968408/150‑1 (MQ=255)
                              aaTCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAA‑TTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCAt                                                                                                                         <  1:3709793/150‑1 (MQ=255)
                              aaTCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAA‑TTTTACTAAACTCCCCAGCAATTACACAAAATACCATCACCAt                                                                                                                         >  1:283169/1‑150 (MQ=255)
                                tCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAA‑TTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGa                                                                                                                       <  2:3556269/150‑1 (MQ=255)
                                tCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAA‑TTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGa                                                                                                                       <  2:3557127/150‑1 (MQ=255)
                                 ccccTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAA‑TTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGaa                                                                                                                      <  1:2473299/150‑1 (MQ=255)
                                      tACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAA‑TTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGtt                                                                                                                 <  2:9553/150‑1 (MQ=255)
                                      tACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAA‑TTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGtt                                                                                                                 <  1:2599726/150‑1 (MQ=255)
                                       acacacTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAA‑TTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTc                                                                                                                >  2:3899416/1‑150 (MQ=255)
                                       acacacTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAA‑TTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTc                                                                                                                >  1:2964031/1‑150 (MQ=255)
                                           acTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAA‑TTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCAAGAATGGTTCCgct                                                                                                            >  2:2400671/1‑148 (MQ=255)
                                             tGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTGTAATTTAGTGATACAGTTAAAGTAAAATCATTTAACTGCAACTTTTTATTAAAAA‑TATTACTAAAAAACCCAGCCATTACACAAACAACCAACACCATGAAAGTTTACGAttt                                                                                                          >  1:3165406/1‑150 (MQ=255)
                                                ccGTCACTCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAA‑TTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTtctc                                                                                                       <  2:2581306/150‑1 (MQ=255)
                                                 cGGCTATCGTTTTTGCCGGAGAGTGATGCCGTTTATTTTCGTGATCAAGTTTAAGTAAATGCATTTAGCTGCTACTTTTTAGTAAAAA‑TTTTACTAAACTCCCCAGCAATTACACAATCTACCATCACCATGAATGGTTCCGATTtctct                                                                                                      <  1:4217021/150‑1 (MQ=255)
                                                 cGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAA‑TTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTtctct                                                                                                      <  1:4522284/150‑1 (MQ=255)
                                                   gCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAA‑TTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCAGATTTctctct                                                                                                    >  2:4144378/1‑150 (MQ=255)
                                                      aTCGTTTTTGCCTGACAGTGCTTCCGTTTATTTTCTTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAA‑TTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTAcc                                                                                                 <  2:1405079/150‑1 (MQ=255)
                                                      aTCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAATTAAATGCATTTACCTGCTACTTTTTAGTAAAAA‑TTTTACTAACCTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTAcc                                                                                                 >  2:3707369/1‑150 (MQ=255)
                                                      aTCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAA‑TTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTAcc                                                                                                 <  2:3289839/150‑1 (MQ=255)
                                                           ttttGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAA‑TTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAg                                                                                            <  2:835793/150‑1 (MQ=255)
                                                             ttGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAA‑TTTTACTAAACTCCCCAGCAATTACACAAACTAACATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGc                                                                                          >  2:168313/1‑150 (MQ=255)
                                                                ccGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTACATGCATTTACCTGCTACTTTTTAGTAAAAA‑TTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCt                                                                                       <  2:714354/150‑1 (MQ=255)
                                                                      aGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAA‑TTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAAt                                                                                 <  1:4320900/150‑1 (MQ=255)
                                                                            gcCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAA‑TTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTgg                                                                           >  1:2181580/1‑150 (MQ=255)
                                                                            gcCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAA‑TTTTACTAAACTACCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTgg                                                                           <  1:2779357/150‑1 (MQ=255)
                                                                               gTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAA‑TTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGaa                                                                        >  1:1085514/1‑150 (MQ=255)
                                                                                 ttATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAA‑TTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGaaaa                                                                      >  2:3291331/1‑150 (MQ=255)
                                                                                   aTTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAA‑TTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACa                                                                    <  2:2680267/150‑1 (MQ=255)
                                                                                    ttttCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAA‑TTTTACTAAACTACCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACAt                                                                   <  1:1448471/150‑1 (MQ=255)
                                                                                     tttCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAA‑TTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACAtt                                                                  >  2:2238847/1‑150 (MQ=255)
                                                                                     tttCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAA‑TTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACAtt                                                                  >  2:4151689/1‑150 (MQ=255)
                                                                                      ttCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAA‑TTTTACTAAACTCCCCAGAAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTc                                                                 >  2:1249349/1‑150 (MQ=255)
                                                                                      ttCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAA‑TTTTACTAAACTACCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTc                                                                 <  2:3168125/150‑1 (MQ=255)
                                                                                         gTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAA‑TTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGc                                                              >  2:236762/1‑150 (MQ=255)
                                                                                                agaaaaaGTAAATTCATTTAACTGATACTTTATAGTAAAAA‑TTTAAATAAACACCCCAGCAAATACACAAACTACCATCACCATGAAATGTTCCGATATCTCTCTACAGGGAGGCCCAATGAATCTCGGGGAAAACATTCAGAACACCCa                                                       >  2:3212655/5‑150 (MQ=255)
                                                                                                aGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAA‑TTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCa                                                       >  2:3838317/1‑150 (MQ=255)
                                                                                                aGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAA‑TTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCa                                                       <  2:3131357/150‑1 (MQ=255)
                                                                                                aGTTAAAGTAAATGCATTTACCTGCTACTTTTCAGTAAAAA‑TTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCa                                                       <  2:2297621/150‑1 (MQ=255)
                                                                                                  ttAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAA‑TTTTACTAAACTACCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACg                                                     >  1:1731428/1‑150 (MQ=255)
                                                                                                   tAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAA‑TTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTTGGAAAACATTCAGCTCACCCACGa                                                    <  1:3688318/150‑1 (MQ=255)
                                                                                                   tAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAA‑TTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGa                                                    >  1:2773887/1‑150 (MQ=255)
                                                                                                   tAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAA‑TTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGa                                                    >  1:3904742/1‑150 (MQ=255)
                                                                                                    aaaGTAAATGCATTTACCTGCTACTTTTTAGTAAAAA‑TTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGaa                                                   >  1:519153/1‑150 (MQ=255)
                                                                                                      aGTAAATGCATTTACCTGCTACTTTTTAGTAAAAA‑TTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGaaaa                                                 <  1:1860150/150‑1 (MQ=255)
                                                                                                         aaaTGCATTTACCTGCTACTTTTTAGTAAAAA‑TTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCg                                              >  1:3586847/1‑150 (MQ=255)
                                                                                                          atttaaTTTATCTTATACTTTTTAGTAATAA‑TTTTAATAAACTCCCCTGCAATTACACAAACTACCATCACCTTGATTGGTTCCGTTTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGa                                             <  2:283169/145‑1 (MQ=255)
                                                                                                             gCATTTACCTGCTACTTTTTAGTAAAAA‑TTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACtt                                          >  1:4106100/1‑150 (MQ=255)
                                                                                                              cATTTACCTGCTACTTTTTAGTAAAAA‑TTTTCCGAAACTCCCCAGCAATTACACAAACGACCATCACCATGAATGGTTCCGATTTCTCTATACCGGGAGGCCCTAGGAAGCGCGGGGAAAACATTCTGCTCTCCCACGACAACCGCCTTg                                         >  2:3985790/1‑150 (MQ=255)
                                                                                                              cATTTACCTGCTACTTTTTAGTAAAAA‑TTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTg                                         <  1:956486/150‑1 (MQ=255)
                                                                                                              cATTTACCTGCTACTTTTTAGTAAAAA‑TTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTg                                         >  2:2991870/1‑150 (MQ=255)
                                                                                                                  tACCTGATACTTTTTAGTAAAAA‑TTTTAATAAACACCACAGCAATTACACAAACTACCATCACCATGAATGGTTCAGATTTCTCTCTACCGGGAGGACCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGcgcc                                     >  2:1679236/1‑150 (MQ=255)
                                                                                                                    ccTGCTACTTTTTAGTAAAAAGTTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGcgccg                                    >  2:347548/1‑150 (MQ=255)
                                                                                                                    ccTGCTACTTTTTAGTAAAAA‑TTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGcgccgc                                   <  2:3401443/150‑1 (MQ=255)
                                                                                                                    ccTGCTACTTTTTAGTAAAAA‑TTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGcgccgc                                   <  2:1121794/150‑1 (MQ=255)
                                                                                                                      tNCTACTTTTTAGTAAAAA‑TTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCgcgt                                 >  1:2342510/1‑150 (MQ=255)
                                                                                                                       gCTACTTTTTAGTAAAAA‑TTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCgcgtg                                >  1:559609/1‑150 (MQ=255)
                                                                                                                       gCTACTTTTTAGTAAAAA‑TTTTACTAAACTCCCAAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCgcgtg                                >  1:2264531/1‑150 (MQ=255)
                                                                                                                        cTACTTTTTAGGAAAAA‑GTTTACTAAACTCCCCAGCACTTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAAGCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCgcgtgc                               >  2:4329693/1‑150 (MQ=255)
                                                                                                                           cTTTTTAGTAAAAA‑TTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTa                            <  2:4073516/150‑1 (MQ=255)
                                                                                                                            tttttAGTAAAAA‑TTTTACTAAACTACCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTAc                           >  1:3238294/1‑150 (MQ=255)
                                                                                                                                  gTAAAAA‑TTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACtttttt                     >  1:3177008/1‑150 (MQ=255)
                                                                                                                                  gTAAAAA‑TTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACtttttt                     >  1:2549796/1‑150 (MQ=255)
                                                                                                                                  gTAAAAA‑TTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACtttttt                     >  2:1569273/1‑150 (MQ=255)
                                                                                                                                    aaaaa‑TTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTc                   <  2:990107/150‑1 (MQ=255)
                                                                                                                                    aaaaa‑TTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTc                   >  1:750484/1‑150 (MQ=255)
                                                                                                                                    aaaaa‑TTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTc                   <  2:990023/150‑1 (MQ=255)
                                                                                                                                      aaa‑TTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCat                 >  1:3522023/1‑150 (MQ=255)
                                                                                                                                        a‑TTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCatat               <  1:747238/150‑1 (MQ=255)
                                                                                                                                        a‑TTTTACTAAACTACCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTCTTTTCatat               <  2:1625560/150‑1 (MQ=255)
                                                                                                                                           tttACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGa             >  2:1055750/1‑150 (MQ=255)
                                                                                                                                            ttACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGAt            >  2:372058/1‑150 (MQ=255)
                                                                                                                                            ttACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGAt            <  1:3764149/150‑1 (MQ=255)
                                                                                                                                              aCTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTc          <  1:1046117/150‑1 (MQ=255)
                                                                                                                                               cTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCt         <  1:3710559/150‑1 (MQ=255)
                                                                                                                                               cTAAACTACCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCt         >  2:1101426/1‑150 (MQ=255)
                                                                                                                                                     tCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTgcg   >  1:1028099/1‑150 (MQ=255)
                                                                                                                                                      ccccAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTgcgc  >  2:238536/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
CAAATTAAAACTGCGCGGCACGACCCGTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAA‑TTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGC  >  CP009273/3215778‑3216076

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A