Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F121 I0 R1 856 273.6 9081716 94.1% 8545894 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,215,927 C→A 13.4% intergenic (+119/‑65) ebgR → / → ebgA transcriptional repressor/evolved beta‑D‑galactosidase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,215,9270CA13.4% 668.4 / 58.3 220intergenic (+119/‑65)ebgR/ebgAtranscriptional repressor/evolved beta‑D‑galactosidase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (103/84);  new base A (23/10);  total (126/94)
Fisher's exact test for biased strand distribution p-value = 1.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 224 total aligned reads displayed.

AAATTAAAACTGCGCGGCACGACCCGTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGC  >  CP009273/3215779‑3216076
                                                                                                                                                    |                                                                                                                                                     
  aTTAAAACTGCGCGGCACGACCCGTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCAGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTcccc                                                                                                                                                    <  2:4115371/150‑1 (MQ=255)
    tAAAACTGCGCGGCACGACCCGTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTGTATTTTCGTGGTCCCGTTAAAGTAAATGCCGTTACCGGCGACTGTTTAGTAAAAATTTTACGAAACAcccccg                                                                                                                                                  >  1:2055691/1‑148 (MQ=255)
       aaCTGCGCGGCACGACCCGTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCAGTTACCTGCGACTGTTTAGTAAAAATTTTACTAAACTCCCCAGCaa                                                                                                                                               >  2:598523/1‑150 (MQ=255)
       aaCTGCGCGGCACGACCCGTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTAGTGATCCAGGTAAAGTAAATGCATTTACCTGCTACTTTTTAGTACAAATTTTACTAAAAAACCCAGCaa                                                                                                                                               >  2:3931002/1‑150 (MQ=255)
        aCTGCGCGGCACGACCCGTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAAt                                                                                                                                              <  1:3924455/150‑1 (MQ=255)
         cTGCGCGGCACGACCCGTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTACCCAGCAAtt                                                                                                                                             <  2:2174815/150‑1 (MQ=255)
         cTGCGCGGCACGACCCGTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTAGTGATACAGTTAAAGTAAATTCATTTAACTGCTACATTTTATTAAAAATTTTACTAAACTCCCCAGCAAta                                                                                                                                             >  1:4100454/1‑149 (MQ=255)
             gcgGCACGACCCGTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTacac                                                                                                                                         <  1:2640454/150‑1 (MQ=255)
              cgGCACGACCCGTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTGGTGATGCAGTTAAATTAAATTCATTTAACTGCAAATTTTTAGTTAAAAATTTAATAAAAAACCCAGCAAATaaaaa                                                                                                                                        >  1:3670363/1‑146 (MQ=255)
                gCACGACCCTTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACaaa                                                                                                                                      <  2:4506643/150‑1 (MQ=255)
                  aCGACCCGTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACt                                                                                                                                    <  2:2070371/150‑1 (MQ=255)
                       ccGTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTAGTGATACAGTTAAAGTAAATTCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCAt                                                                                                                               >  2:4534834/1‑150 (MQ=255)
                        cGTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATc                                                                                                                              <  2:674454/150‑1 (MQ=255)
                             aaTCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTACCCAGCAATTACACAAACTACCATCACCAt                                                                                                                         <  1:4458749/150‑1 (MQ=255)
                              aTCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTACCCAGCAATTACACAAACTACCATCACCATg                                                                                                                        <  2:35790/150‑1 (MQ=255)
                                 cccTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTAGTGATCCAGTTAAAGTAAATTCATTTACCATCTACTTTTTTTTAAAAATTTTAATAAACAACCCAGCAATTAAAAAAAAAACCATAACCAAGAAt                                                                                                                     >  1:685450/1‑150 (MQ=255)
                                  ccTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATg                                                                                                                    <  2:2802357/150‑1 (MQ=255)
                                     tACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAAAGCATTTACCTGGGACGTTTTAGTAAAAATTTTACTAAACACCCCAGCAATTTCACAAACAAACCACACCAAGAAAgggt                                                                                                                 >  1:3772933/1‑148 (MQ=255)
                                       cacacTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTGTAATTTCGGGTTACCGGTAAATTAAAAGCAATTAACAGCGACATTTTAGTTAAAAATTTTCTAAAAACCCCACCAAATAAACCAACAACCATAAACAAGAATGGttac                                                                                                               >  2:1544135/1‑148 (MQ=255)
                                        acacTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCg                                                                                                              <  1:1060147/150‑1 (MQ=255)
                                         cacTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGGTAAAGTAAAAGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAAATCCCCAGCAATTAAACAAACTAACAACACCAAGAATGGTTCCGa                                                                                                             >  2:950706/1‑150 (MQ=255)
                                            tGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGAttt                                                                                                          <  1:3011794/150‑1 (MQ=255)
                                              tCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTtct                                                                                                        <  1:2525399/150‑1 (MQ=255)
                                              tCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTtct                                                                                                        <  2:3728216/150‑1 (MQ=255)
                                              tCCGGCAATCGTTTGTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTtct                                                                                                        <  1:1501984/150‑1 (MQ=255)
                                                  gCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTctctct                                                                                                    <  1:2897859/150‑1 (MQ=255)
                                                   cAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAAAGCATTTACCTTCTACTTTTTATTAAAAATTTTAATAAACACCCCAGCAATTACAAAAACTACCATCACCATGAATTGTTACGATTTATCTCTa                                                                                                   >  2:2166700/1‑150 (MQ=255)
                                                        gTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCggg                                                                                              <  2:4256708/150‑1 (MQ=255)
                                                        gTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCggg                                                                                              <  2:4256628/150‑1 (MQ=255)
                                                        gTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCAGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTAAACAAACAACCATCACCAAGAATGGTTCCGATTTATCTCTACCggg                                                                                              >  2:3688229/1‑150 (MQ=255)
                                                             tGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAAATTTACTAAACTACCCAGCAATTAAACAAACTACCATCACCAAGAATGGTTCCGATTTCTCTCTACCCGGAGGcc                                                                                         >  2:314508/1‑150 (MQ=255)
                                                             tGCCGGACAGTGCTGCCGTTTATTTTCGTGATACAGTTAAAGTAAATTCATTTACCTGCTACTTTTTATTAAAAATTTTAATAAAATACCCAGCAATTAAAAAAACTACCATCACCATTAAATGTTCCGATTTGTATCTACCGGGAGGcc                                                                                         >  2:4465861/1‑150 (MQ=255)
                                                                 ggACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTAt                                                                                     <  2:1898563/150‑1 (MQ=255)
                                                                 ggACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTAt                                                                                     <  1:2416202/150‑1 (MQ=255)
                                                                     aGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAAAGCATTTAACTGCTACATTTTAGTAAAAATTTTAATAAACTCCCCACCAAATACACAAACAAACAACACCATGAAAGGTTCCGAATTAACTCTACCCGGAGGCCCTATGAAt                                                                                 >  2:2987957/1‑150 (MQ=255)
                                                                      gTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATc                                                                                <  1:340243/150‑1 (MQ=255)
                                                                         ctgcCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCt                                                                             <  1:2987957/150‑1 (MQ=255)
                                                                         ctgcCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTACGATTTCTCTCTAAAGGGAGGCCCAATGAATAGCt                                                                             >  2:2319388/1‑150 (MQ=255)
                                                                         ctgcCGTTTATTTTCGTGATACAGTTAAAGTAAATGCATTTACATGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACAATCACCATGAATGGTTCAGATTTCTCTCTAACGGGAGGCCCTATGAATAGCt                                                                             >  2:3391410/1‑150 (MQ=255)
                                                                                 tATTTTCGTGATCCAGTTAAAGTAAATGCATATACATGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCAGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAAc                                                                     >  1:3074169/1‑150 (MQ=255)
                                                                                        gTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGc                                                              >  2:3755487/1‑150 (MQ=255)
                                                                                        gTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGc                                                              >  1:3236002/1‑150 (MQ=255)
                                                                                         tGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCt                                                             >  1:3326659/1‑150 (MQ=255)
                                                                                            tCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCAc                                                          >  1:731476/1‑150 (MQ=255)
                                                                                             ccAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCAcc                                                         >  2:322963/1‑150 (MQ=255)
                                                                                             ccAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCAcc                                                         >  2:2919710/1‑150 (MQ=255)
                                                                                              cAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCAccc                                                        <  1:4020833/150‑1 (MQ=255)
                                                                                              cAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCAccc                                                        >  1:3298938/1‑150 (MQ=255)
                                                                                               aGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCa                                                       >  2:1965297/1‑150 (MQ=255)
                                                                                               aGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCa                                                       <  2:1586008/150‑1 (MQ=255)
                                                                                               aGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCa                                                       >  1:3438680/1‑150 (MQ=255)
                                                                                                gTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCAc                                                      >  1:3509767/1‑150 (MQ=255)
                                                                                                 ttAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTACTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACg                                                     >  1:3638320/1‑150 (MQ=255)
                                                                                                  tAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGa                                                    >  2:1278116/1‑150 (MQ=255)
                                                                                                    aaGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGaaa                                                  >  2:381574/1‑150 (MQ=255)
                                                                                                     aGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGaaaa                                                 >  1:1059637/1‑150 (MQ=255)
                                                                                                     aGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGaaaa                                                 <  1:4179803/150‑1 (MQ=255)
                                                                                                      gTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAAc                                                >  2:2763819/1‑150 (MQ=255)
                                                                                                       tAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAAcc                                               >  1:1183931/1‑150 (MQ=255)
                                                                                                       tAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAAcc                                               >  2:3226947/1‑150 (MQ=255)
                                                                                                       tAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAAcc                                               <  2:1344347/150‑1 (MQ=255)
                                                                                                       tAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAAcc                                               >  1:190627/1‑150 (MQ=255)
                                                                                                         taTGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGa                                             >  1:100112/2‑150 (MQ=255)
                                                                                                          aTGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGAc                                            >  2:118120/1‑150 (MQ=255)
                                                                                                            gCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACtt                                          >  1:195865/1‑150 (MQ=255)
                                                                                                             cATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTg                                         <  1:1482348/150‑1 (MQ=255)
                                                                                                              aTTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTgc                                        <  2:4047996/150‑1 (MQ=255)
                                                                                                              aTTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTgc                                        >  1:2326012/1‑150 (MQ=255)
                                                                                                              aTTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTgc                                        >  1:2740429/1‑150 (MQ=255)
                                                                                                              aTTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTgc                                        >  1:3692107/1‑150 (MQ=255)
                                                                                                                ttACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTgcgc                                      >  2:4318928/1‑150 (MQ=255)
                                                                                                                ttACCTGCTACTTTTTAGTAAAAATTTTACTAAACTACCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTgcgc                                      >  2:3035932/1‑150 (MQ=255)
                                                                                                                 tACCTGCTACTTTTTAGTAAAAATTTTACTAAACTACCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGcgcc                                     >  2:2057980/1‑150 (MQ=255)
                                                                                                                  aCCTGCTACTTTTTAGTAAAAATTTTACTAAACTACCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGcgccg                                    >  2:1630607/1‑150 (MQ=255)
                                                                                                                   ccTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGcgccgc                                   >  1:299544/1‑150 (MQ=255)
                                                                                                                     tGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCgcgt                                 >  2:1178072/1‑150 (MQ=255)
                                                                                                                       cTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCgcgtgc                               >  1:3611449/1‑150 (MQ=255)
                                                                                                                         aCTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCgcgtgcgt                             <  1:1728451/150‑1 (MQ=255)
                                                                                                                         aCTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCgcgtgcgt                             >  2:2689269/1‑150 (MQ=255)
                                                                                                                          cTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTa                            <  1:1584018/150‑1 (MQ=255)
                                                                                                                                aGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACttttt                      >  2:133617/1‑150 (MQ=255)
                                                                                                                                   aaaaaTTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTc                   <  1:4221178/150‑1 (MQ=255)
                                                                                                                                    aaaaTTTTACTAAACTCCCCATCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCa                  <  1:1267883/150‑1 (MQ=255)
                                                                                                                                    aaaaTTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCa                  <  2:2782585/150‑1 (MQ=255)
                                                                                                                                    aaaaTTTTACTAAACTACCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCa                  <  2:2584071/150‑1 (MQ=255)
                                                                                                                                     aaaTTTTACTAAACTACCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCat                 >  2:3596225/1‑150 (MQ=255)
                                                                                                                                      aaTTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCata                >  1:4347334/1‑150 (MQ=255)
                                                                                                                                      aaTTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCata                >  2:2431891/1‑150 (MQ=255)
                                                                                                                                      aaTTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCata                <  1:950706/150‑1 (MQ=255)
                                                                                                                                       aTTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCatat               <  2:3643980/150‑1 (MQ=255)
                                                                                                                                       aTTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCatat               >  2:1465196/1‑150 (MQ=255)
                                                                                                                                           tACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGAtt           >  2:3028485/1‑150 (MQ=255)
                                                                                                                                            aCTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTc          >  1:2022983/1‑150 (MQ=255)
                                                                                                                                            aCTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCAAGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTc          >  2:326268/1‑150 (MQ=255)
                                                                                                                                              tAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTg        >  1:2570505/1‑150 (MQ=255)
                                                                                                                                              tAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTg        >  2:770917/1‑150 (MQ=255)
                                                                                                                                               aaaCTACCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGt       >  2:1419939/1‑150 (MQ=255)
                                                                                                                                                  cTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTgc    >  1:697965/1‑150 (MQ=255)
                                                                                                                                                   tCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTgcg   >  2:1609386/1‑150 (MQ=255)
                                                                                                                                                    ccccAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTgcgc  >  2:1202544/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
AAATTAAAACTGCGCGGCACGACCCGTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGC  >  CP009273/3215779‑3216076

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A