Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F202 I0 R1 1397 275.7 9089454 95.2% 8653160 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,491,279 G→T 37.4% R166S (CGT→AGT)  alaC ← valine‑pyruvate aminotransferase 3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,491,2790GT37.4% 382.0 / 284.5 277R166S (CGT→AGT) alaCvaline‑pyruvate aminotransferase 3
Reads supporting (aligned to +/- strand):  ref base G (79/94);  new base T (54/50);  total (133/144)
Fisher's exact test for biased strand distribution p-value = 3.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 277 total aligned reads displayed.

CGGCATAGGCCAGGTCATGGACCACCAGCACATCGTAGCGTTTCGCCAGCGCCACTACTTTTTCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGATCGGGTAACTTGGATTCGGCACCAGCACCGTGTCACCATGATCCAGCGTCGCC  >  CP009273/2491132‑2491427
                                                                                                                                                   |                                                                                                                                                    
cGGCATAGGCCAGGTCATGGACCACCAGCACATCGTAGCGTTTCGCCAGCGCCACTACTTTTTCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACTaa                                                                                                                                                    >  2:1946044/1‑150 (MQ=255)
cGGCATAGGCCAGGTCATGGACCACCAGCACATCGTAGCGTTTCGCCAGCGCCACTACTTTTTCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGaa                                                                                                                                                    >  1:132222/1‑150 (MQ=255)
   cATAGGCCAGGTCATGGACCACCAGCACATCGTAGCGTTTCGCCAGCGCCACTACTTTTTCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATgg                                                                                                                                                 <  1:2959928/150‑1 (MQ=255)
      aGGCCAGGTCATGGACCACCAGCACATCGTAGCGTTTCGCCAGCGCCACTACTTTTTCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGgctc                                                                                                                                              <  2:1374768/150‑1 (MQ=255)
       ggCCAGGTCATGGACCACCAGCACATCGTAGCGTTTCGCCAGCGCCACTACTTTTTCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACTAATGgctcg                                                                                                                                             >  1:4054869/1‑150 (MQ=255)
         ccAGGTCATGGACCACCAGCACATCGTAGCGTTTCGCCAGCGCCACTACTTTTTCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACTAATGgctcgct                                                                                                                                           >  1:2719250/1‑150 (MQ=255)
           aGGTCATGGACCACCAGCACATCGTAGCGTTTCGCCAGCGCCACTACTTTTTCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTcc                                                                                                                                         <  1:1923019/150‑1 (MQ=255)
            ggTCATGGACCACCAGCACATCGTAGCGTTTCTCCAGCGCCACTACTTTTTCGAAGAACTCCAGTTCCACGCATTGCGCGGTGGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACTAATGGCTCGCTCCa                                                                                                                                        >  1:145409/1‑150 (MQ=255)
             gTCATGGACCACCAGCACATCGTAGCGTTTCGCCAGCGCCACTACTTTTTCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAg                                                                                                                                       >  1:2082759/1‑150 (MQ=255)
               cATGGTCCACCAGCACATCGTAGCGTTTCGCCAGCGCCACTACTTTTTCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGtt                                                                                                                                     <  1:4406001/150‑1 (MQ=255)
               cATGGACCACCAGCACATCGTAGCGTTTCGCCAGCGCCACTACTTTTTCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACTAATGGCTCGCTCCAGtt                                                                                                                                     <  1:109898/150‑1 (MQ=255)
               cATGGACCACCAGCACATCGTAGCGTTTCGCCAGCGCCACTACTTTTTCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGtt                                                                                                                                     <  1:4405318/150‑1 (MQ=255)
                aTGGACCACCAGCACATCGTAGCGTTTCGCCAGCGCCACTACTTTTTCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTc                                                                                                                                    >  1:2799919/1‑150 (MQ=255)
                  ggACCACCAGCACATCGTAGCGTTTCGCCAGCGCCACTACTTTTTCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGt                                                                                                                                  >  2:1517179/1‑150 (MQ=255)
                     ccaccaGCACATCGTAGCGTTTCGCCAGCGCCACTACTTTTTCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTga                                                                                                                               >  2:1606251/1‑150 (MQ=255)
                       accaGCACATCGTAGCGTTTCGCCAGCGCCACTACTTTTTCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTgaag                                                                                                                             >  1:3277805/1‑150 (MQ=255)
                        ccaGCACATCGTAGCGTTTCGCCAGCGCCACTACTTTTTCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACTAATGGCTCGCTCCAGTTCGTTgaaga                                                                                                                            <  1:2763800/150‑1 (MQ=255)
                        ccaGCACATCGTAGCGTTTCGCCAGCGCCACTACTTTTTCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTgaaga                                                                                                                            <  1:2466944/150‑1 (MQ=255)
                         caGCACATCGTAGCGTTTCGCCAGCGCCACTACTTTTTCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACTAATGGCTCGCTCCAGTTCGTTgaagaa                                                                                                                           >  2:3916177/1‑150 (MQ=255)
                          aGCACATCGTAGCGTTTCGCCAGCGCCACTACTTTTTCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGaaa                                                                                                                          >  2:467416/1‑150 (MQ=255)
                          aGCACATCGTAGCGTTTCGCCAGCGCCACTACTTTTTCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGaaa                                                                                                                          >  2:467426/1‑150 (MQ=255)
                                 cGTAGCGTTTCGCCAGCGCCACTACTTTTTCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGacac                                                                                                                   <  1:1632420/150‑1 (MQ=255)
                                 cGTAGCGTTTCGCCAGCGCCACTACTTTTTCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGacac                                                                                                                   >  2:1255673/1‑150 (MQ=255)
                                 cGTAGCGTTTCGCCAGCGCCACTACTTTTTCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGacac                                                                                                                   >  2:3967926/1‑150 (MQ=255)
                                   tAGCGTTTCGCCAGCGCCACTACTTTTTCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCt                                                                                                                 >  1:3989724/1‑150 (MQ=255)
                                      cGTTTCGCCAGCGCCACTACTTTTTCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACTAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTcc                                                                                                              <  2:2914104/150‑1 (MQ=255)
                                       gTTTCGCCAGCGCCACTACTTTTTCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTcca                                                                                                             >  2:3488765/1‑150 (MQ=255)
                                         ttCGCCAGCGCCACTACTTTTTCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTccacc                                                                                                           <  1:4540136/150‑1 (MQ=255)
                                          tCGCCAGCGCCACTACTTTTTCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTccacca                                                                                                          <  1:1757029/150‑1 (MQ=255)
                                             ccAGCGCCACTACTTTTTCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCg                                                                                                       <  1:4083218/150‑1 (MQ=255)
                                               aGCGCCACTACTTTTTCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGc                                                                                                     <  1:1606251/150‑1 (MQ=255)
                                               aGCGCCACTACTTGTTCGAAGCACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGc                                                                                                     <  2:4083998/150‑1 (MQ=255)
                                                 cgcCACTACTTTTTCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACTAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCAc                                                                                                   <  2:350531/150‑1 (MQ=255)
                                                  gcCACTACTTTTTCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACt                                                                                                  <  1:977720/150‑1 (MQ=255)
                                                    cACTACTTTTTCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACTAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGa                                                                                                >  2:657686/1‑150 (MQ=255)
                                                     actactTTTTCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACTAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAg                                                                                               >  1:2782361/1‑150 (MQ=255)
                                                      ctactTTTTCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACTAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGc                                                                                              >  1:2542126/1‑150 (MQ=255)
                                                         ctTTTTCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACTAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTa                                                                                           >  1:1871295/1‑150 (MQ=255)
                                                          tttttCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTAc                                                                                          <  1:1699345/150‑1 (MQ=255)
                                                           ttttCGAAGAACTCCAGTTCCACGCATGGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTAcc                                                                                         <  2:3226349/150‑1 (MQ=255)
                                                            tttCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCt                                                                                        >  2:116109/1‑150 (MQ=255)
                                                              tCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACGTTCACTAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTgc                                                                                      >  2:2861957/1‑150 (MQ=255)
                                                                gaagaaCTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACTAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTgcgc                                                                                    >  2:4360402/1‑150 (MQ=255)
                                                                   gaaCTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACTAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGCGCGTACCTGCGcccc                                                                                 >  2:2306710/1‑150 (MQ=255)
                                                                   gaaCTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACTAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGcccc                                                                                 >  2:3453824/1‑150 (MQ=255)
                                                                    aaCTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACTAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCg                                                                                >  2:2643767/1‑150 (MQ=255)
                                                                    aaCTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACTAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCg                                                                                <  2:2136422/150‑1 (MQ=255)
                                                                    aaCTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCg                                                                                <  2:1137525/150‑1 (MQ=255)
                                                                       tCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCa                                                                             <  1:4226252/150‑1 (MQ=255)
                                                                          aGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATc                                                                          >  1:3735884/1‑150 (MQ=255)
                                                                          aGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATc                                                                          >  2:3391721/1‑150 (MQ=255)
                                                                           gTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCa                                                                         >  1:3963807/1‑150 (MQ=255)
                                                                             tCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCAcc                                                                       <  2:1007972/150‑1 (MQ=255)
                                                                              ccACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACTAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACcg                                                                      <  1:2213960/150‑1 (MQ=255)
                                                                              ccACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACcg                                                                      >  2:534726/1‑150 (MQ=255)
                                                                               cACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACcgc                                                                     >  1:3426166/1‑150 (MQ=255)
                                                                                   cATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACTAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGACAATCACCGCGCCa                                                                 >  1:1600187/1‑150 (MQ=255)
                                                                                   cATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCa                                                                 >  2:910522/1‑150 (MQ=255)
                                                                                    aTTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCAt                                                                <  2:1480408/150‑1 (MQ=255)
                                                                                    aTTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCAt                                                                >  1:701513/1‑150 (MQ=255)
                                                                                     ttGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATa                                                               <  2:247378/150‑1 (MQ=255)
                                                                                         gcgGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCGATAAata                                                           <  2:2053750/150‑1 (MQ=255)
                                                                                         gcgGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCGATAAata                                                           <  2:3579388/150‑1 (MQ=255)
                                                                                          cgGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAatat                                                          <  2:3235678/150‑1 (MQ=255)
                                                                                            gTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACTAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATgg                                                        <  2:3798299/150‑1 (MQ=255)
                                                                                                 gTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGATCgg                                                   <  2:1787742/150‑1 (MQ=255)
                                                                                                  tttGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGATCggg                                                  <  2:423027/150‑1 (MQ=255)
                                                                                                    tGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACTAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGATCGGGTa                                                <  1:1883295/150‑1 (MQ=255)
                                                                                                      aCGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGATCGGGTAAc                                              <  1:999606/150‑1 (MQ=255)
                                                                                                       cGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGATCGGGTAACt                                             <  2:738855/150‑1 (MQ=255)
                                                                                                        gggAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGATCGGGTAACtt                                            <  1:4258264/150‑1 (MQ=255)
                                                                                                        gggAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGATCGGGTAACtt                                            <  1:4257987/150‑1 (MQ=255)
                                                                                                          gAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGATCGGGTAACTTgg                                          <  2:188328/150‑1 (MQ=255)
                                                                                                           aaGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACTAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGATCGGGTAACTTGGa                                         >  2:1239444/1‑150 (MQ=255)
                                                                                                             gCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGATCGGGTAACTTGGAtt                                       <  2:769695/150‑1 (MQ=255)
                                                                                                                  ggATCATCATCTTCGGTTTCGGATAACTTTCACTAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGATCGGGTAACTTGGATTCGGCa                                  <  1:3710817/150‑1 (MQ=255)
                                                                                                                  ggATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGATCGGGTAACTTGGATTCGGCa                                  >  2:3686164/1‑150 (MQ=255)
                                                                                                                   gATCATCATCTTCGGTTTCGGATAACTTTCACTAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGATCGGGTAACTTGGATTCGGCAc                                 <  1:2643767/150‑1 (MQ=255)
                                                                                                                    atcatcatcTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGATCGGGTAACTTGGATTCGGCAcc                                <  2:874655/150‑1 (MQ=255)
                                                                                                                        tcatcTTCGGTTTCGGATAACTTTCACTAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGATCGGGTAACTTGGATTCGGCACCAGCa                            >  1:2615619/1‑150 (MQ=255)
                                                                                                                         catcTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGATCGGGTAACTTGGATTCGGCACCAGCAc                           >  1:234021/1‑150 (MQ=255)
                                                                                                                          atcTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGATCGGGTAACTTGGATTCGGCACCAGCAcc                          >  2:2724381/1‑150 (MQ=255)
                                                                                                                          atcTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGATCGGGTAACTTGGATTCGGCACCAGCAcc                          >  2:2721039/1‑150 (MQ=255)
                                                                                                                               cGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGATCGGGTAACTTGGATTCGGCACCAGCACCGTGTc                     >  2:4495886/1‑150 (MQ=255)
                                                                                                                                ggTTTCGGATAACTTTCACTAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGATCGGGTAACTTGGATTCGGCACCAGCACCGTGTCa                    <  1:4360995/150‑1 (MQ=255)
                                                                                                                                 gTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGATCGGGTAACTTGGATTCGGCACCAGCACCGTGTCAc                   <  1:732488/150‑1 (MQ=255)
                                                                                                                                    tCGGATAACTTTCACTAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGATCGGGTAACTTGGATTCGGCACCAGCACCGTGTCACCAt                <  2:2391483/150‑1 (MQ=255)
                                                                                                                                    tCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGATCGGGTAACTTGGATTCGGCACCAGCACCGTGTCACCAt                >  1:3532124/1‑150 (MQ=255)
                                                                                                                                        aTAACTTTCACTAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGATCGGGTAACTTGGATTCGGCACCAGCACCGTGTCACCATGATc            <  1:382322/150‑1 (MQ=255)
                                                                                                                                         tAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGATCGGGTAACTTGGATTCGGCACCAGCACCGTGTCACCATGATcc           <  2:168656/150‑1 (MQ=255)
                                                                                                                                          aaCTTTCACTAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGATCGGGTAACTTGGATTCGGCACCAGCACCGTGTCACCATGATCCa          <  2:2195435/150‑1 (MQ=255)
                                                                                                                                           aCTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCTACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGATCGGGTCACGTGCATTCGGCACGAGCAGCGTGTCACCATGATCCAg         >  2:4410140/1‑150 (MQ=255)
                                                                                                                                           aCTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGATCGGGTAACTTGGATTCGGCACCAGCACCGTGTCACCATGATCCAg         <  1:566186/150‑1 (MQ=255)
                                                                                                                                           aCTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGATCGGGTAACTTGGATTCGGCACCAGCACCGTGTCACCATGATCCAg         <  1:566122/150‑1 (MQ=255)
                                                                                                                                             tttCACTAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGATCGGGTAACTTGGATTCGGCACCAGCACCGTGTCACCATGATCCAGCg       >  2:4539734/1‑150 (MQ=255)
                                                                                                                                             tttCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGATCGGGTAACTTGGATTCGGCACCAGCACCGTGTCACCATGATCCAGCg       >  1:1241994/1‑150 (MQ=255)
                                                                                                                                               tCACTAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGATCGGGTAACTTGGATTCGGCACCAGCACCGTGTCACCATGATCCAGCGTc     <  2:1108964/150‑1 (MQ=255)
                                                                                                                                               tCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGATCGGGTAACTTGGATTCGGCACCAGCACCGTGTCACCATGATCCAGCGTc     <  2:3308162/150‑1 (MQ=255)
                                                                                                                                                cACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGATCGGGTAACTTGGATTCGGCACCAGCACCGTGTCACCATGATCCAGCGTCg    >  1:4371068/1‑150 (MQ=255)
                                                                                                                                                  cGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGATCGGGTAACTTGGATTCGGCACCAGCACCGTGTCACCATGATCCAGCGTCGcc  >  2:607760/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CGGCATAGGCCAGGTCATGGACCACCAGCACATCGTAGCGTTTCGCCAGCGCCACTACTTTTTCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGATCGGGTAACTTGGATTCGGCACCAGCACCGTGTCACCATGATCCAGCGTCGCC  >  CP009273/2491132‑2491427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A