Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F218 I0 R1 1506 278.7 9275112 94.5% 8764980 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,491,279 G→T 16.4% R166S (CGT→AGT)  alaC ← valine‑pyruvate aminotransferase 3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,491,2790GT16.4% 783.4 / 94.9 249R166S (CGT→AGT) alaCvaline‑pyruvate aminotransferase 3
Reads supporting (aligned to +/- strand):  ref base G (103/105);  new base T (22/19);  total (125/124)
Fisher's exact test for biased strand distribution p-value = 7.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

Only 100 of 252 total aligned reads displayed.

GGCATAGGCCAGGTCATGGACCACCAGCACATCGTAGCGTTTCGCCAGCGCCACTACTTTTTCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGATCGGGTAACTTGGATTCGGCACCAGCACCGTGTCACCATGATCCAGCGTCGCCA  >  CP009273/2491133‑2491428
                                                                                                                                                  |                                                                                                                                                     
ggCATAGGCCAGGTCATGGACCACCAGCACATCGTAGCGTTTCGCCAGCGCCACTACTTTTTCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACTAAt                                                                                                                                                    >  1:2420408/1‑150 (MQ=255)
ggCATAGGCCAGGTCATGGACCACCAGCACATCGTAGCGTTTCGCCAGCGCCACTACTTTTTCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAAt                                                                                                                                                    <  1:3806011/150‑1 (MQ=255)
ggCATAGGCCAGGTCATGGACCACCAGCACATCGGAGCGTTTCGCCAGCGCCACTACTTTTTCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAAt                                                                                                                                                    <  2:127685/150‑1 (MQ=255)
 gCATAGGCCAGGTCATGGACCACCAGCACATCGTAGCGTTTCGCCAGCGCCACTACTTTTTCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATg                                                                                                                                                   <  1:171760/150‑1 (MQ=255)
  cATAGGCCAGGTCATGGACCACCAGCACATCGTAGCGTTTCGCCAGCGCCACTACTTTTTCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATgg                                                                                                                                                  >  1:4216787/1‑150 (MQ=255)
  cATAGGCCAGGTCATGGACCACCAGCACATCGTAGCGTTTCGCCAGCGCCACTACTTTTTCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATgg                                                                                                                                                  <  1:2579482/150‑1 (MQ=255)
    tAGGCCAGGTCATGGACCACCAGCACATCGTAGCGTTTCGCCAGCGCCACTACTTTTTCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGgct                                                                                                                                                >  2:2868191/1‑150 (MQ=255)
     aGGCCAGGTCATGGACCACCAGCACATCGTAGCGTTTCGCCAGCGCCACTACTTTTTCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGgctc                                                                                                                                               >  1:1312030/1‑150 (MQ=255)
         cAGGTCATGGACCACCAGCACATCGTAGCGTTTCGCCAGCGCCACTACTTTTTCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGgctcgctc                                                                                                                                           >  2:3917588/1‑150 (MQ=255)
         cAGGTCATGGACCACCAGCACATCGTAGCGTTTCGCCAGCGCCACTACTTTTTCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGgctcgctc                                                                                                                                           >  2:3475217/1‑150 (MQ=255)
           ggTCATGGACCACCAGCACATCGTAGCGTTTCGCCAGCGCCACTACTTTTTCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCa                                                                                                                                         <  1:1760335/150‑1 (MQ=255)
              cATGGACCACCAGCACATCGTAGCGTTTCGCCAGCGCCACTACTTTTTCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACTAATGGCTCGCTCCAGtt                                                                                                                                      >  2:1879542/1‑150 (MQ=255)
              cATGGACCACCAGCACATCGTAGCGTTTCGCCAGCGCCACTACTTTTTCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACTAATGGCTCGCTCCAGtt                                                                                                                                      <  1:1563281/150‑1 (MQ=255)
               aTGGACCACCAGCACATCGTAGCGTTTCGCCAGCGCCACTACTTTTTCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTc                                                                                                                                     >  2:3341713/1‑150 (MQ=255)
                tGGACCACCAGCACATCGTAGCGTTTCGCCAGCGCCACTACTTTTTCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCg                                                                                                                                    <  1:4244550/150‑1 (MQ=255)
                  gACCACCAGCACATCGTAGCGTTTCGCCAGCGCCACTACTTTTTCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACTAATGGCTCGCTCCAGTTCGtt                                                                                                                                  <  1:2836725/150‑1 (MQ=255)
                  gACCACCAGCACATCGTAGCGTTTCGCCAGCGCCACTACTTTTTCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGtt                                                                                                                                  <  2:1198781/150‑1 (MQ=255)
                   accaccAGCACATCGTAGCGTTTCGCCAGCGCCACTACTTTTTCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTg                                                                                                                                 <  2:2282882/150‑1 (MQ=255)
                    ccaccaGCACATCGTAGCGTTTCGCCAGCGCCACTACTTTTTCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTga                                                                                                                                <  1:4440424/150‑1 (MQ=255)
                         aGCACATCGTAGCGTTTCGCCAGCGCCACTACTTTTTCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGaaa                                                                                                                           <  2:2452220/150‑1 (MQ=255)
                          gCACATCGTAGCGTTTCGCCAGCGCCACTACTTTTTCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAAt                                                                                                                          <  1:408374/150‑1 (MQ=255)
                           cacaTCGTAGCGTTTCGCCAGCGCCACTACTTTTTCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATc                                                                                                                         >  1:980529/1‑150 (MQ=255)
                             caTCGTAGCGTTTCGCCAGCGCCACTACTTTTTCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACTAATGGCTCGCTCCAGTTCGTTGAAGAAATCGa                                                                                                                       >  2:215037/1‑150 (MQ=255)
                                 gTAGCGTTTCGCCAGCGCCACTACTTTTTCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGacaca                                                                                                                   <  1:1691359/150‑2 (MQ=255)
                                  tAGCGTTTCGCCAGCGCCACTACTTTTTCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCt                                                                                                                  <  1:3453938/150‑1 (MQ=255)
                                   aGCGTTTCGCCAGCGCCACTACTTTTTCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACTAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCtt                                                                                                                 >  2:2563722/1‑150 (MQ=255)
                                   aGCGTTTCGCCAGCGCCACTACTTTTTCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCtt                                                                                                                 <  2:219489/150‑1 (MQ=255)
                                     cGTTTCGCCAGCGCCACTACTTTTTCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACTAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTcc                                                                                                               <  1:2951957/150‑1 (MQ=255)
                                     cGTTTCGCCAGCGCCACTACTTTTTCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTcc                                                                                                               <  2:2743569/150‑1 (MQ=255)
                                        ttCGCCAGCGCCACTACTTTTTCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTccacc                                                                                                            >  1:3404106/1‑150 (MQ=255)
                                          cGCCAGCGCCACTACTTTTTCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAg                                                                                                          <  2:2974015/150‑1 (MQ=255)
                                          cGCCAGCGCCACTACTTTTTCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAg                                                                                                          <  1:3875460/150‑1 (MQ=255)
                                           gCCAGCGCCACTACTTTTTCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGc                                                                                                         >  1:2068700/1‑150 (MQ=255)
                                             cAGCGCCACTACTTTTTCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCgg                                                                                                       >  2:792795/1‑150 (MQ=255)
                                              aGCGCCACTACTTTTTCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGc                                                                                                      >  1:1983476/1‑150 (MQ=255)
                                                cgcCACTACTTTTTCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCAc                                                                                                    <  2:1637926/150‑1 (MQ=255)
                                                 gcCACTACTTTTTCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACt                                                                                                   <  2:3821759/150‑1 (MQ=255)
                                                   cACTACTTTTTCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGa                                                                                                 <  1:4461974/150‑1 (MQ=255)
                                                   cACTACTTTTTCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGa                                                                                                 >  2:466677/1‑150 (MQ=255)
                                                   cACTACTTTTTCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGa                                                                                                 >  1:1034693/1‑150 (MQ=255)
                                                    actacaTTATCGAAGAACTCCAGTTCCACGCATTTCTCGGTTGTGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCAAGAATGGCTCGCTACAGTTCGTTGAAGACATCGACACCTTCCACCAGCGGCACagag                                                                                                >  2:349609/1‑150 (MQ=255)
                                                      tactTTTTCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCA‑CAGCGGCACTGAGCGt                                                                                             >  2:4178603/1‑150 (MQ=255)
                                                       actTTTTCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGt                                                                                             <  2:371809/150‑1 (MQ=255)
                                                        ctTTTTCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTa                                                                                            >  1:3053935/1‑150 (MQ=255)
                                                         tttttCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGATTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTAc                                                                                           <  2:1750313/150‑1 (MQ=255)
                                                          ttttCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTAcc                                                                                          >  1:3936005/1‑150 (MQ=255)
                                                           tttCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCt                                                                                         <  1:2106310/150‑1 (MQ=255)
                                                             tCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTgc                                                                                       >  1:2260593/1‑150 (MQ=255)
                                                             tCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTgc                                                                                       >  1:2260560/1‑150 (MQ=255)
                                                                 agaaCTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACTAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGccc                                                                                   >  1:1130170/1‑150 (MQ=255)
                                                                 agaaCTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGTATCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGccc                                                                                   >  2:776174/1‑150 (MQ=255)
                                                                 agaaCTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGccc                                                                                   >  2:4088510/1‑150 (MQ=255)
                                                                  gaaCTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGATGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGcccc                                                                                  <  1:2465768/150‑1 (MQ=255)
                                                                   aaCTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCg                                                                                 >  1:75228/1‑150 (MQ=255)
                                                                   aaCTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCg                                                                                 >  2:3803315/1‑150 (MQ=255)
                                                                   aaCTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCg                                                                                 >  2:1609376/1‑150 (MQ=255)
                                                                      tCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACTAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCa                                                                              >  2:2102426/1‑150 (MQ=255)
                                                                      tCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCa                                                                              >  1:4289417/1‑150 (MQ=255)
                                                                       ccAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACTAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCaa                                                                             >  1:823898/1‑150 (MQ=255)
                                                                          gTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCa                                                                          >  1:261669/1‑150 (MQ=255)
                                                                            tCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACTAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCAcc                                                                        >  1:856714/1‑150 (MQ=255)
                                                                                 gCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGcc                                                                   <  1:4508498/150‑1 (MQ=255)
                                                                                  cATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACTAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCa                                                                  <  1:4574399/150‑1 (MQ=255)
                                                                                   aTTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACTAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCAt                                                                 >  1:1996573/1‑150 (MQ=255)
                                                                                   aTTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCAt                                                                 >  1:642511/1‑150 (MQ=255)
                                                                                         cgGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAatat                                                           <  1:2591145/150‑1 (MQ=255)
                                                                                             tGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGAt                                                       <  1:898610/150‑1 (MQ=255)
                                                                                             tGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGAt                                                       >  1:717271/1‑150 (MQ=255)
                                                                                             tGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGAt                                                       <  1:3216368/150‑1 (MQ=255)
                                                                                              gggTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGATc                                                      <  1:572069/150‑1 (MQ=255)
                                                                                              gggTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGATc                                                      <  1:1396261/150‑1 (MQ=255)
                                                                                               ggTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGATCg                                                     <  2:2221125/150‑1 (MQ=255)
                                                                                                gTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGATCgg                                                    <  1:35107/150‑1 (MQ=255)
                                                                                                gTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGATCgg                                                    <  2:663594/150‑1 (MQ=255)
                                                                                                      cGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGATCGGGTAACt                                              >  1:3628071/1‑150 (MQ=255)
                                                                                                       gggAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGATCGGGTAACtt                                             >  2:1051846/1‑150 (MQ=255)
                                                                                                       gggAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGATCGGGTAACtt                                             >  2:3329631/1‑150 (MQ=255)
                                                                                                          aaGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGATCGGGTAACTTGGa                                          >  2:1384973/1‑150 (MQ=255)
                                                                                                          aaGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGATCGGGTAACTTGGa                                          >  2:323346/1‑150 (MQ=255)
                                                                                                            gCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCCATCACCGCGCCATAAATATGGATCGGGTAACTTGGAtt                                        >  2:1521099/1‑150 (MQ=255)
                                                                                                             ccGAGGATCATCATCTTCGGTTTCGGATAACTTTCACTAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGATCGGGTAACTTGGATTc                                       <  1:3977737/150‑1 (MQ=255)
                                                                                                              cGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGATCGGGTAACTTGGATTCg                                      >  2:3564984/1‑150 (MQ=255)
                                                                                                               gAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGATCGGGTAACTTGGATTCgg                                     >  2:1669386/1‑150 (MQ=255)
                                                                                                                    tcatcatcTTCGGTTTCGGATAACTTTCACTAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGATCGGGTAACTTGGATTCGGCACCa                                <  1:3059417/150‑1 (MQ=255)
                                                                                                                    tcatcatcTTCGGTTTCGGATAACTTTCACTAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGATCGGGTAACTTCGATTCGGCACCa                                <  2:2644013/150‑1 (MQ=255)
                                                                                                                      atcatcTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGATCGGGTAACTTGGATTCGGCACCAGc                              >  2:469775/1‑150 (MQ=255)
                                                                                                                       tcatcTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGATCGGGTAACTTGGATTCGGCACCAGCa                             <  1:3882186/150‑1 (MQ=255)
                                                                                                                          tcTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGATCGGGTAACTTGGATTCGGCACCAGCACCg                          >  1:991938/1‑150 (MQ=255)
                                                                                                                            ttCGGTTTCGGATAACTTTCACTAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGATCGGGTAACTTGGATTCGGCACCAGCACCgtg                        <  2:1805/150‑1 (MQ=255)
                                                                                                                             tCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGATCGGGTAACTTGGATTCGGCACCAGCACCgtgt                       <  2:4426927/150‑1 (MQ=255)
                                                                                                                             tCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGATCGGGTAACTTGGATTCGGCACCAGCACCgtgt                       <  2:4426978/150‑1 (MQ=255)
                                                                                                                              cGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGATCGGGTAACTTGGATTCGGCACCAGCACCGTGTc                      >  2:320161/1‑150 (MQ=255)
                                                                                                                                 tttCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGATCGGGTAACTTGGATTCGGCACCAGCACCGTGTCAcc                   <  2:853746/150‑1 (MQ=255)
                                                                                                                                 tttCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGATCGGGTAACTTGGATTCGGCACCAGCACCGTGTCAcc                   >  2:351753/1‑150 (MQ=255)
                                                                                                                                      gATAACTTTCACTAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGATCGGGTAACTTGGATTCGGCACCAGCACCGTGTCACCATGAt              <  2:1151425/150‑1 (MQ=255)
                                                                                                                                       aTAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGATCGGGTAACTTGGATTCGGCACCAGCACCGTGTCACCATGATc             <  1:474739/150‑1 (MQ=255)
                                                                                                                                        tAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGATCGGGTAACTTGGATTCGGCACCAGCACCGTGTCACCATGATcc            <  1:1837416/150‑1 (MQ=255)
                                                                                                                                         aaCTTTCACGAATGGCTCGATCCAGTTCGTTTAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCTCCCCGGCAATCACCGCGCCATAAATATGGATCGGGTAACTTGGATTCGGCACCAGCAACGTGTAACCATGATCCa           <  2:562414/150‑1 (MQ=255)
                                                                                                                                              tCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGATCGGGTAACTTGGATTCGGCACCAGCACCGTGTCACCATGATCCAGCGTc      >  1:4442233/1‑150 (MQ=255)
                                                                                                                                                  gAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGATCGGGTAACTTGGATTCGGCACCAGCACCGTGTCACCATGATCCAGCGTCGCCa  <  1:3328531/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
GGCATAGGCCAGGTCATGGACCACCAGCACATCGTAGCGTTTCGCCAGCGCCACTACTTTTTCGAAGAACTCCAGTTCCACGCATTGCGCGGTTGGGTTTGACGGGAAGCCGAGGATCATCATCTTCGGTTTCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGATCGGGTAACTTGGATTCGGCACCAGCACCGTGTCACCATGATCCAGCGTCGCCA  >  CP009273/2491133‑2491428

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A