Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F121 I0 R1 856 273.6 9081716 94.1% 8545894 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,993,211 C→A 87.0% V14L (GTG→TTG)  ygeR ← LysM domain‑containing M23 family putative peptidase; septation lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,993,2110CA87.0% 630.8 / 89.4 236V14L (GTG→TTG) ygeRLysM domain‑containing M23 family putative peptidase; septation lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (15/16);  new base A (117/88);  total (132/104)
Fisher's exact test for biased strand distribution p-value = 4.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 236 total aligned reads displayed.

TTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAGGTTAATTT  >  CP009273/2993066‑2993357
                                                                                                                                                 |                                                                                                                                                  
  tACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATAcc                                                                                                                                              <  1:1954892/150‑1 (MQ=255)
     gCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCag                                                                                                                                           <  1:3149372/150‑1 (MQ=255)
      cTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCaga                                                                                                                                          >  1:432298/1‑150 (MQ=255)
         gTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGAt                                                                                                                                       >  2:2978886/1‑150 (MQ=255)
         gTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGAt                                                                                                                                       >  1:2417422/1‑150 (MQ=255)
            cccGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGAtttt                                                                                                                                    <  2:3368614/150‑1 (MQ=255)
            cccGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGAtttt                                                                                                                                    <  2:3368771/150‑1 (MQ=255)
               gtggtgCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTa                                                                                                                                 >  2:2449370/1‑150 (MQ=255)
                  gtgCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTc                                                                                                                              <  1:3623434/150‑1 (MQ=255)
                    gcgcGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAg                                                                                                                            <  1:1934250/150‑1 (MQ=255)
                     cgcgAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAgg                                                                                                                           >  2:3882748/1‑150 (MQ=255)
                     cgcgAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAgg                                                                                                                           <  2:1609213/150‑1 (MQ=255)
                     cgcgAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGAGAACAACATCAAGATACCCAGAGATTTTTTATTCAgg                                                                                                                           >  2:4170736/1‑150 (MQ=255)
                        gAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGt                                                                                                                        <  1:1164676/150‑1 (MQ=255)
                         aaaTACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTc                                                                                                                       >  1:3736867/1‑150 (MQ=255)
                          aaTACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTcc                                                                                                                      <  2:588017/150‑1 (MQ=255)
                           aTACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTccc                                                                                                                     <  2:2650189/150‑1 (MQ=255)
                           aTACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTccc                                                                                                                     >  1:1275151/1‑150 (MQ=255)
                           aTACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTccc                                                                                                                     <  1:2671178/150‑1 (MQ=255)
                            tACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCg                                                                                                                    >  2:3604681/1‑150 (MQ=255)
                                atatAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACt                                                                                                                <  2:3122852/150‑1 (MQ=255)
                                 tataGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTc                                                                                                               >  1:2326188/1‑150 (MQ=255)
                                  ataGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCa                                                                                                              >  2:1650614/1‑150 (MQ=255)
                                      cGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAAt                                                                                                          >  2:802041/1‑150 (MQ=255)
                                      cGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAAt                                                                                                          >  1:2063628/1‑150 (MQ=255)
                                      cGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAAt                                                                                                          >  1:3214598/1‑150 (MQ=255)
                                        tATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCt                                                                                                        <  2:1786/150‑1 (MQ=255)
                                         aTCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATACCAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCtt                                                                                                       >  2:2300567/1‑150 (MQ=255)
                                         aTCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCtt                                                                                                       >  2:4132186/1‑150 (MQ=255)
                                           ccccccGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTc                                                                                                     >  2:616158/1‑150 (MQ=255)
                                             ccccGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCt                                                                                                   >  2:562123/1‑150 (MQ=255)
                                             ccccGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCt                                                                                                   <  2:3928223/150‑1 (MQ=255)
                                             ccccGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCt                                                                                                   <  2:1224069/150‑1 (MQ=255)
                                                  tttCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCtt                                                                                              >  2:2406399/1‑150 (MQ=255)
                                                   ttCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTa                                                                                             >  1:518598/1‑150 (MQ=255)
                                                          tgtAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACa                                                                                      >  1:3911043/1‑150 (MQ=255)
                                                              aaCGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGAc                                                                                  >  2:1982665/1‑150 (MQ=255)
                                                              aaCGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGAc                                                                                  >  2:1982819/1‑150 (MQ=255)
                                                              aaCGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTCTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGAc                                                                                  <  1:752326/150‑1 (MQ=255)
                                                               aCGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACg                                                                                 >  1:2692263/1‑150 (MQ=255)
                                                                cGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGc                                                                                >  1:1098586/1‑150 (MQ=255)
                                                                cGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGc                                                                                >  1:3177989/1‑150 (MQ=255)
                                                                cGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGc                                                                                >  1:1586784/1‑150 (MQ=255)
                                                                        gAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCAt                                                                        <  2:1353450/150‑1 (MQ=255)
                                                                                 ccTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGcagccag                                                               <  1:2654943/150‑1 (MQ=255)
                                                                                    gTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCct                                                            <  1:3921423/150‑1 (MQ=255)
                                                                                    gTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCct                                                            >  2:4406078/1‑150 (MQ=255)
                                                                                     tATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCctc                                                           <  1:1327125/150‑1 (MQ=255)
                                                                                      aTCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCctct                                                          >  1:383433/1‑150 (MQ=255)
                                                                                       tCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCtt                                                         >  1:3766784/1‑150 (MQ=255)
                                                                                       tCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCtt                                                         <  1:3063588/150‑1 (MQ=255)
                                                                                          gatgatTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGAt                                                      >  1:1109217/1‑150 (MQ=255)
                                                                                          gatgatTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGAt                                                      >  2:3736175/1‑150 (MQ=255)
                                                                                            tgatTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATAc                                                    <  2:4283143/150‑1 (MQ=255)
                                                                                             gatTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATAcc                                                   >  2:653907/1‑150 (MQ=255)
                                                                                              atTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATAccc                                                  >  1:1444919/1‑150 (MQ=255)
                                                                                                 tGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAAt                                               >  2:2817196/1‑150 (MQ=255)
                                                                                                    tACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAAtctc                                            >  2:254040/1‑150 (MQ=255)
                                                                                                      cccGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCtt                                          >  2:283405/1‑150 (MQ=255)
                                                                                                      cccGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGACTCTTGATACCCAATCTCtt                                          <  1:974583/150‑1 (MQ=255)
                                                                                                        cGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGc                                        <  2:1574946/150‑1 (MQ=255)
                                                                                                        cGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGc                                        <  2:3242566/150‑1 (MQ=255)
                                                                                                             aGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTg                                   >  1:4523492/1‑150 (MQ=255)
                                                                                                             aGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTg                                   >  1:386355/1‑150 (MQ=255)
                                                                                                              gCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCTCTTAAAAAACAAGACGCCTTATAATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTgg                                  <  1:2661707/150‑1 (MQ=255)
                                                                                                               cccGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGa                                 >  1:441212/1‑150 (MQ=255)
                                                                                                               cccGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGa                                 <  1:566239/150‑1 (MQ=255)
                                                                                                                ccGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGaa                                <  1:1899212/150‑1 (MQ=255)
                                                                                                                  gCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGaaaa                              >  2:2913974/1‑150 (MQ=255)
                                                                                                                    cAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAAc                            >  2:3771391/1‑150 (MQ=255)
                                                                                                                    cAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAAc                            <  2:4218818/150‑1 (MQ=255)
                                                                                                                    cAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGaaaatc                            >  2:1884966/1‑148 (MQ=255)
                                                                                                                       aaGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTg                         <  2:2678288/150‑1 (MQ=255)
                                                                                                                          cAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTa                      <  1:2954704/150‑1 (MQ=255)
                                                                                                                           aGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTaa                     >  1:4483559/1‑150 (MQ=255)
                                                                                                                           aGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTaa                     >  1:4484672/1‑150 (MQ=255)
                                                                                                                           aGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTaa                     <  1:1239564/150‑1 (MQ=255)
                                                                                                                            gTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAAt                    >  2:2318034/1‑150 (MQ=255)
                                                                                                                            gTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAAt                    >  2:51478/1‑150 (MQ=255)
                                                                                                                             tCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATa                   <  2:77021/150‑1 (MQ=255)
                                                                                                                             tCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATa                   <  2:80677/150‑1 (MQ=255)
                                                                                                                             tCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATa                   >  2:1189778/1‑150 (MQ=255)
                                                                                                                             tCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATa                   <  1:3384376/150‑1 (MQ=255)
                                                                                                                              ccAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATag                  >  1:160986/1‑150 (MQ=255)
                                                                                                                                  ccGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGaa              >  2:394163/1‑150 (MQ=255)
                                                                                                                                   cGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGaaa             >  1:665852/1‑150 (MQ=255)
                                                                                                                                   cGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGaaa             >  2:465214/1‑150 (MQ=255)
                                                                                                                                    gATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAag            <  2:3567966/150‑1 (MQ=255)
                                                                                                                                     aTAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCGTCTGGAAAAACGTGGTAATAGAGAAaga           >  1:370059/1‑150 (MQ=255)
                                                                                                                                      tAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAagag          >  1:2902561/1‑150 (MQ=255)
                                                                                                                                      tAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAagag          >  1:2987696/1‑150 (MQ=255)
                                                                                                                                      tAACAACATCAAGAGACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAagag          <  2:852717/150‑1 (MQ=255)
                                                                                                                                         caacaTCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGTGGtt       >  2:306033/1‑150 (MQ=255)
                                                                                                                                         caacaTCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAGGtt       >  1:551528/1‑150 (MQ=255)
                                                                                                                                         caacaTCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAGGtt       >  2:102533/1‑150 (MQ=255)
                                                                                                                                          aacaTCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAGGTTa      >  1:2191840/1‑150 (MQ=255)
                                                                                                                                            caTCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAGGTTAAt    <  2:2761464/150‑1 (MQ=255)
                                                                                                                                             aTCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAGGTTAAtt   <  2:456761/150‑1 (MQ=255)
                                                                                                                                             aTCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAGGTTAAtt   <  1:2369880/150‑1 (MQ=255)
                                                                                                                                              tCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAGGTTAAttt  <  1:934170/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
TTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAGGTTAATTT  >  CP009273/2993066‑2993357

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAGGTTAATTTCAT  >  CP009273/2993062‑2993360
                                                                                                                                                     |                                                                                                                                                     
TTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAA                                                                                                                                                       >  SRR19973080.922139/1‑150 (MQ=60)
    TTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATA                                                                                                                                                   <  SRR19973080.3606221/150‑1 (MQ=60)
      TACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACC                                                                                                                                                 <  SRR19973080.1965970/150‑1 (MQ=60)
         GCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAG                                                                                                                                              <  SRR19973080.3166721/150‑1 (MQ=60)
          CTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGA                                                                                                                                             <  SRR19973080.709062/150‑1 (MQ=60)
          CTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGA                                                                                                                                             >  SRR19973080.434974/1‑150 (MQ=60)
          CTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGA                                                                                                                                             >  SRR19973080.434990/1‑150 (MQ=60)
            GGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGA                                                                                                                                           >  SRR19973080.1403493/1‑150 (MQ=60)
             GTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGAT                                                                                                                                          >  SRR19973080.3194230/1‑150 (MQ=60)
             GTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGAT                                                                                                                                          >  SRR19973080.2430839/1‑150 (MQ=60)
             GTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGAT                                                                                                                                          >  SRR19973080.151922/1‑150 (MQ=60)
                CCCGTGGTGCGCGAAATACGATAGAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTT                                                                                                                                       <  SRR19973080.2025145/150‑1 (MQ=60)
                      GTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTC                                                                                                                                 <  SRR19973080.3721282/150‑1 (MQ=60)
                      GTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTC                                                                                                                                 <  SRR19973080.3643462/150‑1 (MQ=60)
                      GTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTC                                                                                                                                 <  SRR19973080.1427707/150‑1 (MQ=60)
                        GCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAG                                                                                                                               <  SRR19973080.1945187/150‑1 (MQ=60)
                        GCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAG                                                                                                                               <  SRR19973080.1945137/150‑1 (MQ=60)
                            GAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGT                                                                                                                           <  SRR19973080.1171590/150‑1 (MQ=60)
                             AAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTC                                                                                                                          >  SRR19973080.3757298/1‑150 (MQ=60)
                              AATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCC                                                                                                                         <  SRR19973080.4552306/150‑1 (MQ=60)
                               ATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCC                                                                                                                        <  SRR19973080.2685709/150‑1 (MQ=60)
                               ATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCC                                                                                                                        >  SRR19973080.1282872/1‑150 (MQ=60)
                                TACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCG                                                                                                                       <  SRR19973080.2981835/150‑1 (MQ=60)
                                     TATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTC                                                                                                                  >  SRR19973080.2339109/1‑150 (MQ=60)
                                          CGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAAT                                                                                                             >  SRR19973080.3232327/1‑150 (MQ=60)
                                          CGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAAT                                                                                                             >  SRR19973080.1212306/1‑150 (MQ=60)
                                          CGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAAT                                                                                                             >  SRR19973080.2075325/1‑150 (MQ=60)
                                                CCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCC                                                                                                       <  SRR19973080.2299422/150‑1 (MQ=60)
                                                  CCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTC                                                                                                     >  SRR19973080.96513/1‑150 (MQ=60)
                                                  CCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTC                                                                                                     >  SRR19973080.96529/1‑150 (MQ=60)
                                                       TTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTA                                                                                                >  SRR19973080.521904/1‑150 (MQ=60)
                                                              TGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACA                                                                                         >  SRR19973080.3932634/1‑150 (MQ=60)
                                                              TGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACA                                                                                         >  SRR19973080.3932705/1‑150 (MQ=60)
                                                               GTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAA                                                                                        >  SRR19973080.1954665/1‑150 (MQ=60)
                                                                  AACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTCTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGAC                                                                                     <  SRR19973080.756578/150‑1 (MQ=60)
                                                                   ACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACG                                                                                    >  SRR19973080.2706982/1‑150 (MQ=60)
                                                                   ACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACG                                                                                    >  SRR19973080.2706715/1‑150 (MQ=60)
                                                                   ACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACG                                                                                    >  SRR19973080.2706668/1‑150 (MQ=60)
                                                                    CGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGC                                                                                   >  SRR19973080.3195518/1‑150 (MQ=60)
                                                                    CGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGC                                                                                   >  SRR19973080.1104913/1‑150 (MQ=60)
                                                                    CGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGC                                                                                   >  SRR19973080.1596068/1‑150 (MQ=60)
                                                                      GAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCT                                                                                 <  SRR19973080.3726269/150‑1 (MQ=60)
                                                                      GAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCT                                                                                 <  SRR19973080.1778684/150‑1 (MQ=60)
                                                                       AGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTT                                                                                <  SRR19973080.3057035/150‑1 (MQ=60)
                                                                             AATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATA                                                                          <  SRR19973080.3768704/150‑1 (MQ=60)
                                                                              ATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAG                                                                         >  SRR19973080.3288070/1‑150 (MQ=60)
                                                                               TACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGC                                                                        >  SRR19973080.3324904/1‑150 (MQ=60)
                                                                                 CGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAG                                                                      <  SRR19973080.2361105/150‑1 (MQ=60)
                                                                                     CCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAG                                                                  <  SRR19973080.2669305/150‑1 (MQ=60)
                                                                                       TGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCC                                                                <  SRR19973080.2332238/150‑1 (MQ=60)
                                                                                        GTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCT                                                               <  SRR19973080.3943077/150‑1 (MQ=60)
                                                                                         TATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTC                                                              <  SRR19973080.1335130/150‑1 (MQ=60)
                                                                                         TATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTC                                                              <  SRR19973080.2598757/150‑1 (MQ=60)
                                                                                          ATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCT                                                             >  SRR19973080.385637/1‑150 (MQ=60)
                                                                                          ATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCT                                                             >  SRR19973080.385603/1‑150 (MQ=60)
                                                                                           TCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTT                                                            >  SRR19973080.3787293/1‑150 (MQ=60)
                                                                                           TCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTT                                                            <  SRR19973080.778494/150‑1 (MQ=60)
                                                                                           TCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTT                                                            <  SRR19973080.3080236/150‑1 (MQ=60)
                                                                                           TCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTT                                                            <  SRR19973080.3080259/150‑1 (MQ=60)
                                                                                              GATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGAT                                                         >  SRR19973080.1113914/1‑150 (MQ=60)
                                                                                              GATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGAT                                                         >  SRR19973080.1115567/1‑150 (MQ=60)
                                                                                               ATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCTATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATA                                                        <  SRR19973080.2681806/150‑1 (MQ=60)
                                                                                                  ATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCC                                                     >  SRR19973080.1453328/1‑150 (MQ=60)
                                                                                                  ATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCC                                                     <  SRR19973080.3832996/150‑1 (MQ=60)
                                                                                                     TGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAAT                                                  >  SRR19973080.4061503/1‑150 (MQ=60)
                                                                                                        TACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTC                                               >  SRR19973080.1314733/1‑150 (MQ=60)
                                                                                                          CCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTT                                             <  SRR19973080.1774580/150‑1 (MQ=60)
                                                                                                          CCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGACTCTTGATACCCAATCTCTT                                             <  SRR19973080.980484/150‑1 (MQ=60)
                                                                                                            CGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGC                                           >  SRR19973080.652833/1‑150 (MQ=60)
                                                                                                             GAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCT                                          >  SRR19973080.2007166/1‑150 (MQ=60)
                                                                                                             GAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCT                                          >  SRR19973080.259389/1‑150 (MQ=60)
                                                                                                                 AGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTG                                      >  SRR19973080.388566/1‑150 (MQ=60)
                                                                                                                 AGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTG                                      >  SRR19973080.388489/1‑150 (MQ=60)
                                                                                                                 AGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTG                                      >  SRR19973080.4547868/1‑150 (MQ=60)
                                                                                                                  GCCCGCCAAAATCAGTCAAACCGATAACAAAATCAAGATAACCAGAGATTTTTTATTCAGGCGTCCCTCACTCAAAATCTTTCCTCGATTAAAAAACAAGACGCCTTATAATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGG                                     <  SRR19973080.2676132/150‑1 (MQ=60)
                                                                                                                  GCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCTCTTAAAAAACAAGACGCCTTATAATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGG                                     <  SRR19973080.2676140/150‑1 (MQ=60)
                                                                                                                  GCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATAATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGG                                     <  SRR19973080.534228/150‑1 (MQ=60)
                                                                                                                   CCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGA                                    <  SRR19973080.569788/150‑1 (MQ=60)
                                                                                                                   CCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGA                                    >  SRR19973080.443960/1‑150 (MQ=60)
                                                                                                                    CCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAA                                   <  SRR19973080.1909847/150‑1 (MQ=60)
                                                                                                                    CCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAA                                   >  SRR19973080.466463/1‑150 (MQ=60)
                                                                                                                       CCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAA                                >  SRR19973080.4453203/1‑150 (MQ=60)
                                                                                                                       CCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAA                                >  SRR19973080.4454616/1‑150 (MQ=60)
                                                                                                                        CAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAAC                               <  SRR19973080.2156524/150‑1 (MQ=60)
                                                                                                                         AAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACG                              <  SRR19973080.3918913/150‑1 (MQ=60)
                                                                                                                           AAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTG                            >  SRR19973080.319988/1‑150 (MQ=60)
                                                                                                                             GCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGT                          <  SRR19973080.2752473/150‑1 (MQ=60)
                                                                                                                              CAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTA                         <  SRR19973080.2970085/150‑1 (MQ=60)
                                                                                                                              CAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTA                         <  SRR19973080.2970721/150‑1 (MQ=60)
                                                                                                                              CAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTTCTTCTGGAAAAACGTGGTA                         >  SRR19973080.4536700/1‑150 (MQ=60)
                                                                                                                               AGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAA                        <  SRR19973080.1247065/150‑1 (MQ=60)
                                                                                                                               AGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAA                        >  SRR19973080.4508932/1‑150 (MQ=60)
                                                                                                                               AGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAA                        >  SRR19973080.4507953/1‑150 (MQ=60)
                                                                                                                               AGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAA                        >  SRR19973080.4507816/1‑150 (MQ=60)
                                                                                                                                 TCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATA                      <  SRR19973080.1253374/150‑1 (MQ=60)
                                                                                                                                 TCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATA                      <  SRR19973080.3402684/150‑1 (MQ=60)
                                                                                                                                  CCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAG                     >  SRR19973080.984648/1‑150 (MQ=60)
                                                                                                                                  CCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAG                     >  SRR19973080.162271/1‑150 (MQ=60)
                                                                                                                                  CCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAG                     >  SRR19973080.162258/1‑150 (MQ=60)
                                                                                                                                     ACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGA                  <  SRR19973080.1133877/150‑1 (MQ=60)
                                                                                                                                       CGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAA                >  SRR19973080.669824/1‑150 (MQ=60)
                                                                                                                                       CGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAA                >  SRR19973080.669486/1‑150 (MQ=60)
                                                                                                                                         ATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCGTCTGGAAAAACGTGGTAATAGAGAAAGA              >  SRR19973080.372172/1‑150 (MQ=60)
                                                                                                                                          TAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAG             >  SRR19973080.3003808/1‑150 (MQ=60)
                                                                                                                                          TAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAG             >  SRR19973080.2918403/1‑150 (MQ=60)
                                                                                                                                           AACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAGG            <  SRR19973080.3128958/150‑1 (MQ=60)
                                                                                                                                            ACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAGGT           <  SRR19973080.1207047/150‑1 (MQ=60)
                                                                                                                                            ACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAGGT           >  SRR19973080.40525/1‑150 (MQ=60)
                                                                                                                                             CAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAGGTT          >  SRR19973080.555288/1‑150 (MQ=60)
                                                                                                                                             CAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAGGTT          >  SRR19973080.555017/1‑150 (MQ=60)
                                                                                                                                              AACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAGGTTA         >  SRR19973080.2204017/1‑150 (MQ=60)
                                                                                                                                               ACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAGGTTAA        <  SRR19973080.2983403/150‑1 (MQ=60)
                                                                                                                                                 ATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAGGTTAATT      <  SRR19973080.2383064/150‑1 (MQ=60)
                                                                                                                                                 ATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGATTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAGGTTAATT      <  SRR19973080.2383287/150‑1 (MQ=60)
                                                                                                                                                  TCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAGGTTAATTT     <  SRR19973080.3654146/150‑1 (MQ=60)
                                                                                                                                                  TCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAGGTTAATTT     <  SRR19973080.939880/150‑1 (MQ=60)
                                                                                                                                                   CAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAGGTTAATTTC    >  SRR19973080.3315357/1‑150 (MQ=60)
                                                                                                                                                   CAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATATTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAGGTTAATTTC    <  SRR19973080.1330499/150‑1 (MQ=60)
                                                                                                                                                    AAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAGGTTAATTTCA   <  SRR19973080.3504446/150‑1 (MQ=60)
                                                                                                                                                     AGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAGGTTAATTTCAT  <  SRR19973080.388034/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
TTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAGGTTAATTTCAT  >  CP009273/2993062‑2993360

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑