Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F78 I0 R1 626 241.3 10389380 77.7% 8072548 149.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,993,699 C→A 57.9% intergenic (‑449/‑5) ygeR ← / → xdhA LysM domain‑containing M23 family putative peptidase; septation lipoprotein/xanthine dehydrogenase, molybdenum binding subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,993,6990CA57.9% 87.7 / 260.7 184intergenic (‑449/‑5)ygeR/xdhALysM domain‑containing M23 family putative peptidase; septation lipoprotein/xanthine dehydrogenase, molybdenum binding subunit
Reads supporting (aligned to +/- strand):  ref base C (43/35);  new base A (43/63);  total (86/98)
Fisher's exact test for biased strand distribution p-value = 5.38e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 204 total aligned reads displayed.

GTCATCACACTTCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGAACAAGCCAG  >  CP009273/2993552‑2993846
                                                                                                                                                   |                                                                                                                                                   
gTCATCACACTTCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGaataat                                                                                                                                                   <  1:3644647/150‑1 (MQ=255)
gTCATCACACTTCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGaataat                                                                                                                                                   <  2:1898128/150‑1 (MQ=255)
 tcatcaCACTTCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCCTAAGAACCAAATGTTCAAATCGCGGGGCTTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATaatg                                                                                                                                                  >  2:3071249/1‑150 (MQ=255)
  catcaCACTTCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATGTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCAATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATcatgc                                                                                                                                                 >  2:541758/1‑150 (MQ=255)
  catcaCACTTCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAATAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCCACCGCTACGGGTGAATaatgc                                                                                                                                                 >  2:2052522/1‑150 (MQ=255)
  catcaCACTTCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGTAGCACCCGCTACGGGTGAATcatgc                                                                                                                                                 >  2:2914508/1‑150 (MQ=255)
    tcaCACTTCCTCCCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAAGCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATaatgcat                                                                                                                                               <  1:439603/150‑1 (MQ=255)
    tcaCACTTCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATcatgcat                                                                                                                                               <  1:2490153/150‑1 (MQ=255)
    tcaCACTTCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATaatgcat                                                                                                                                               <  1:704208/150‑1 (MQ=255)
    tcaCACTTCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATaatgcat                                                                                                                                               <  2:4760213/150‑1 (MQ=255)
      acacTTCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCatgcatgc                                                                                                                                             <  1:1940869/150‑1 (MQ=255)
      acacTTCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAatgcatgc                                                                                                                                             <  2:2229545/150‑1 (MQ=255)
       cacTTCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTGAATCTTTGCCATAAGAACCACATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATgcg                                                                                                                                            >  2:4626534/1‑150 (MQ=255)
       cacTTCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATgcg                                                                                                                                            >  2:4839962/1‑150 (MQ=255)
       cacTTCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCGTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATgcg                                                                                                                                            >  1:3761081/1‑150 (MQ=255)
            ccTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCg                                                                                                                                       <  2:4147648/150‑1 (MQ=255)
              tACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGAt                                                                                                                                     <  1:1311933/150‑1 (MQ=255)
              tACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGAt                                                                                                                                     >  2:4572123/1‑150 (MQ=255)
                 cTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGcc                                                                                                                                  >  2:1223497/1‑150 (MQ=255)
                  tCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCa                                                                                                                                 <  1:3334011/150‑1 (MQ=255)
                     ccTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATTTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTg                                                                                                                              >  1:826202/1‑150 (MQ=255)
                       tAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCt                                                                                                                            <  2:4747605/150‑1 (MQ=255)
                            cTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGt                                                                                                                       <  1:2529843/150‑1 (MQ=255)
                              tATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCa                                                                                                                     <  1:1964896/150‑1 (MQ=255)
                               aTCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCAc                                                                                                                    <  1:3720751/150‑1 (MQ=255)
                               aTCGTCGTTTCTGGCCTAAATATTTCCTGCTTAGACAAAATCATTGCCATAAGAACCAAATTTTAAAATAGAGGGGATTTATATGGAAACGCGGGAAGCAACCCCTACGGGTGAATCATGCATGCGCGTCGATGCCATTTCTAAGGTCAc                                                                                                                    >  2:2571708/1‑150 (MQ=255)
                                  gtcgtTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACcgg                                                                                                                 >  1:1294562/1‑150 (MQ=255)
                                      tttCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACcgggcgg                                                                                                             >  1:4540758/1‑150 (MQ=255)
                                          tGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCAc                                                                                                         <  1:1018705/150‑1 (MQ=255)
                                          tGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCAc                                                                                                         <  1:190377/150‑1 (MQ=255)
                                           ggCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACg                                                                                                        <  1:3263877/150‑1 (MQ=255)
                                           ggCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACg                                                                                                        <  2:3970018/150‑1 (MQ=255)
                                               tAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGatat                                                                                                    <  2:335299/150‑1 (MQ=255)
                                                aaaTCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGAtata                                                                                                   <  1:2060085/150‑1 (MQ=255)
                                                  aTCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACt                                                                                                 <  1:772070/150‑1 (MQ=255)
                                                  aTCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACt                                                                                                 <  1:3375480/150‑1 (MQ=255)
                                                  aTCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACt                                                                                                 <  1:1461933/150‑1 (MQ=255)
                                                  aTCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACt                                                                                                 <  1:918522/150‑1 (MQ=255)
                                                    cTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGa                                                                                               >  2:4509840/1‑150 (MQ=255)
                                                          tGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGAtta                                                                                         <  1:2210538/150‑1 (MQ=255)
                                                           gCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGAttat                                                                                        >  2:3154961/1‑150 (MQ=255)
                                                            cTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGAttatg                                                                                       >  2:4100377/1‑150 (MQ=255)
                                                             ttAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGAttatgt                                                                                      >  2:3126062/1‑150 (MQ=255)
                                                              tAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCAGTGCTAAGGTCACCGGGCGGGCACGATATACTGACGAttatgtt                                                                                     >  2:2878377/1‑150 (MQ=255)
                                                                   tAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGc                                                                                >  1:2782567/1‑150 (MQ=255)
                                                                   tAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGc                                                                                <  2:4674470/150‑1 (MQ=255)
                                                                        cTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTCTGGCGGGCa                                                                           >  2:1683278/1‑150 (MQ=255)
                                                                        cTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCa                                                                           >  1:1466217/1‑150 (MQ=255)
                                                                        cTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCa                                                                           >  1:4751742/1‑150 (MQ=255)
                                                                         tttGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCAt                                                                          <  2:378094/150‑1 (MQ=255)
                                                                           tGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCAtgt                                                                        >  1:585610/1‑150 (MQ=255)
                                                                           tGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCAtgt                                                                        <  2:2409374/150‑1 (MQ=255)
                                                                              cATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGtt                                                                     <  1:2334341/150‑1 (MQ=255)
                                                                              cATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGtt                                                                     <  2:1461512/150‑1 (MQ=255)
                                                                              cATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGtt                                                                     <  1:4173378/150‑1 (MQ=255)
                                                                               aTAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTa                                                                    >  2:5076214/1‑150 (MQ=255)
                                                                               aTAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTa                                                                    >  2:4291443/1‑150 (MQ=255)
                                                                                 aaGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATg                                                                  >  2:2152705/1‑150 (MQ=255)
                                                                                 aaGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATg                                                                  >  2:2152716/1‑150 (MQ=255)
                                                                                     aCCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGaa                                                              >  1:3499300/1‑150 (MQ=255)
                                                                                      ccAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGaaa                                                             >  2:2139566/1‑150 (MQ=255)
                                                                                       cAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAat                                                            <  2:3268841/150‑1 (MQ=255)
                                                                                         aaTGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAatat                                                          >  2:261707/1‑150 (MQ=255)
                                                                                          aTGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATg                                                         <  2:1417850/150‑1 (MQ=255)
                                                                                          aTGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATg                                                         <  2:1843504/150‑1 (MQ=255)
                                                                                           tGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGt                                                        <  1:1122256/150‑1 (MQ=255)
                                                                                              tCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACg                                                     <  1:3154085/150‑1 (MQ=255)
                                                                                              tCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACg                                                     <  1:5076214/150‑1 (MQ=255)
                                                                                               cAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGt                                                    <  1:657567/150‑1 (MQ=255)
                                                                                                aaaTCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTa                                                   <  2:4499378/150‑1 (MQ=255)
                                                                                                aaaTCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTa                                                   <  2:3805296/150‑1 (MQ=255)
                                                                                                  aTCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGc                                                 <  1:2546856/150‑1 (MQ=255)
                                                                                                   tCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGcc                                                <  1:4607633/150‑1 (MQ=255)
                                                                                                    cGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGccc                                               >  2:1762814/1‑150 (MQ=255)
                                                                                                      aGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTa                                             >  1:1809544/1‑150 (MQ=255)
                                                                                                         ggATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCg                                          <  1:4572123/150‑1 (MQ=255)
                                                                                                          gATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGc                                         >  1:4811398/1‑150 (MQ=255)
                                                                                                             ttCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGcaca                                      <  1:2359179/150‑1 (MQ=255)
                                                                                                              tCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACAt                                     <  1:396660/150‑1 (MQ=255)
                                                                                                               cTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATg                                    <  2:2004356/150‑1 (MQ=255)
                                                                                                                  tGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGtt                                 >  1:4722279/1‑150 (MQ=255)
                                                                                                                    gAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTAt                               <  2:5126182/150‑1 (MQ=255)
                                                                                                                       gcgcGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGcc                            <  1:4291443/150‑1 (MQ=255)
                                                                                                                             gAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGt                      <  2:826202/150‑1 (MQ=255)
                                                                                                                             gAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGGTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGt                      >  2:2098883/1‑150 (MQ=255)
                                                                                                                               aGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTAt                    <  2:2112085/150‑1 (MQ=255)
                                                                                                                                 cAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTa                  >  2:2521015/1‑150 (MQ=255)
                                                                                                                                  aaCCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTaa                 >  1:1621551/1‑150 (MQ=255)
                                                                                                                                    ccGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAatg               <  2:1410066/150‑1 (MQ=255)
                                                                                                                                      gCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAatgat             >  2:3721942/1‑150 (MQ=255)
                                                                                                                                      gCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAatgat             >  2:5094399/1‑150 (MQ=255)
                                                                                                                                      gCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAatgat             <  2:2222766/150‑1 (MQ=255)
                                                                                                                                       cTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAatgatg            >  2:1028513/1‑150 (MQ=255)
                                                                                                                                       cTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAatgatg            <  1:1856190/150‑1 (MQ=255)
                                                                                                                                           gggTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGAACa        <  1:2822948/150‑1 (MQ=255)
                                                                                                                                           gggTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGAACa        >  2:3466676/1‑150 (MQ=255)
                                                                                                                                             gTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGAACAAg      >  2:3732669/1‑150 (MQ=255)
                                                                                                                                               gAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCAGGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGAACAAGcc    >  1:2633667/1‑150 (MQ=255)
                                                                                                                                                aataatGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGAACAAGCCa   <  1:2763388/150‑1 (MQ=255)
                                                                                                                                                aaTCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGAACAAGCCa   <  1:3040333/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GTCATCACACTTCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGAACAAGCCAG  >  CP009273/2993552‑2993846

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTCATCACACTTCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGAACAAGCCA  >  CP009273/2993552‑2993845
                                                                                                                                                   |                                                                                                                                                  
GTCATCACACTTCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAAT                                                                                                                                                  <  SRR19973083.3661320/150‑1 (MQ=60)
GTCATCACACTTCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAAT                                                                                                                                                  <  SRR19973083.1844489/150‑1 (MQ=60)
 TCATCACACTTCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCATGCCTGCTTAGACTAAATCTTTGCCCAAAGAACCAAATGTTCAAATAGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATG                                                                                                                                                 >  SRR19973083.1030221/1‑150 (MQ=60)
    TCACACTTCCTCCCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAAGCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCAT                                                                                                                                              <  SRR19973083.441555/150‑1 (MQ=60)
    TCACACTTCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCAT                                                                                                                                              <  SRR19973083.2502588/150‑1 (MQ=60)
    TCACACTTCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCAT                                                                                                                                              <  SRR19973083.2502865/150‑1 (MQ=60)
    TCACACTTCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCAT                                                                                                                                              <  SRR19973083.441500/150‑1 (MQ=60)
    TCACACTTCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCAT                                                                                                                                              <  SRR19973083.707718/150‑1 (MQ=60)
     CACACTTCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATG                                                                                                                                             <  SRR19973083.3386993/150‑1 (MQ=60)
      ACACTTCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGC                                                                                                                                            <  SRR19973083.1950786/150‑1 (MQ=60)
       CACTTCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCGTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCG                                                                                                                                           >  SRR19973083.3778109/1‑150 (MQ=60)
          TTCCTACCTCCCCTAACGCTTATCGTCGTTTATGGCGTAAATCTTGCCTGCTTAGACTAAATATTTGCCATAAGAACCAAATGTTCAAATCGTGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGT                                                                                                                                        >  SRR19973083.2526387/1‑150 (MQ=60)
           TCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCATTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTC                                                                                                                                       <  SRR19973083.2550625/150‑1 (MQ=60)
            CCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGCAGCAACCGCTACGGGTGAATCATGCATGCGCGTCG                                                                                                                                      >  SRR19973083.4282307/1‑150 (MQ=60)
              TACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGAT                                                                                                                                    <  SRR19973083.1318243/150‑1 (MQ=60)
                  TCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCA                                                                                                                                <  SRR19973083.3349598/150‑1 (MQ=60)
                     CCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATTTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCAAGCGCGTCGATGCCCTTG                                                                                                                             >  SRR19973083.830127/1‑150 (MQ=60)
                            CTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGT                                                                                                                      <  SRR19973083.2542682/150‑1 (MQ=60)
                            CTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGT                                                                                                                      <  SRR19973083.3267299/150‑1 (MQ=60)
                              TATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCA                                                                                                                    <  SRR19973083.1974898/150‑1 (MQ=60)
                              TATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCA                                                                                                                    <  SRR19973083.1974936/150‑1 (MQ=60)
                               ATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCAC                                                                                                                   <  SRR19973083.3737665/150‑1 (MQ=60)
                               ATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTGTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCAC                                                                                                                   <  SRR19973083.3737664/150‑1 (MQ=60)
                                  GTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGG                                                                                                                >  SRR19973083.1300806/1‑150 (MQ=60)
                                  GTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGG                                                                                                                >  SRR19973083.1300824/1‑150 (MQ=60)
                                   TCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGG                                                                                                               <  SRR19973083.4782806/150‑1 (MQ=60)
                                      TTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGG                                                                                                            >  SRR19973083.4560451/1‑150 (MQ=60)
                                          TGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCAC                                                                                                        <  SRR19973083.1023810/150‑1 (MQ=60)
                                          TGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCAC                                                                                                        <  SRR19973083.191450/150‑1 (MQ=60)
                                           GGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACG                                                                                                       <  SRR19973083.3279282/150‑1 (MQ=60)
                                           GGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACG                                                                                                       <  SRR19973083.3279273/150‑1 (MQ=60)
                                               TAAATCGTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATAT                                                                                                   <  SRR19973083.1934251/150‑1 (MQ=60)
                                                AAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATA                                                                                                  <  SRR19973083.2070579/150‑1 (MQ=60)
                                                  ATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACT                                                                                                <  SRR19973083.3391149/150‑1 (MQ=60)
                                                  ATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACT                                                                                                <  SRR19973083.775838/150‑1 (MQ=60)
                                                  ATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACT                                                                                                <  SRR19973083.922948/150‑1 (MQ=60)
                                                  ATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACT                                                                                                <  SRR19973083.1469299/150‑1 (MQ=60)
                                                  ATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACT                                                                                                >  SRR19973083.771463/1‑150 (MQ=60)
                                                  ATATTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTAAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACT                                                                                                <  SRR19973083.1469303/150‑1 (MQ=60)
                                                    CTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGA                                                                                              >  SRR19973083.3077969/1‑150 (MQ=60)
                                                     TTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGAC                                                                                             <  SRR19973083.3768833/150‑1 (MQ=60)
                                                      TGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACG                                                                                            <  SRR19973083.188857/150‑1 (MQ=60)
                                                          TGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTA                                                                                        <  SRR19973083.2221678/150‑1 (MQ=60)
                                                            CTTAGACTAAATCTTTGCCATAAGAACCACATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATG                                                                                      <  SRR19973083.2804465/150‑1 (MQ=60)
                                                            CTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATG                                                                                      <  SRR19973083.2005840/150‑1 (MQ=60)
                                                                 ACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATG                                                                                 >  SRR19973083.4685754/1‑150 (MQ=60)
                                                                   TAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGC                                                                               >  SRR19973083.2796380/1‑150 (MQ=60)
                                                                   TAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGC                                                                               >  SRR19973083.2796397/1‑150 (MQ=60)
                                                                        CTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCA                                                                          >  SRR19973083.1473604/1‑150 (MQ=60)
                                                                        CTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCA                                                                          >  SRR19973083.1473700/1‑150 (MQ=60)
                                                                        CTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCA                                                                          >  SRR19973083.4771831/1‑150 (MQ=60)
                                                                        CTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCA                                                                          >  SRR19973083.4772010/1‑150 (MQ=60)
                                                                         TTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCAT                                                                         <  SRR19973083.500056/150‑1 (MQ=60)
                                                                           TGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGT                                                                       >  SRR19973083.588549/1‑150 (MQ=60)
                                                                            GCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTG                                                                      <  SRR19973083.2080991/150‑1 (MQ=60)
                                                                              CATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTT                                                                    <  SRR19973083.2346310/150‑1 (MQ=60)
                                                                              CATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTT                                                                    <  SRR19973083.2346322/150‑1 (MQ=60)
                                                                              CATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTT                                                                    <  SRR19973083.2346606/150‑1 (MQ=60)
                                                                              CATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTT                                                                    <  SRR19973083.4191766/150‑1 (MQ=60)
                                                                               ATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTA                                                                   <  SRR19973083.706934/150‑1 (MQ=60)
                                                                                TAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTAT                                                                  <  SRR19973083.880762/150‑1 (MQ=60)
                                                                                 AAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATG                                                                 >  SRR19973083.4007519/1‑150 (MQ=60)
                                                                                     ACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAA                                                             >  SRR19973083.3515333/1‑150 (MQ=60)
                                                                                        AAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATA                                                          <  SRR19973083.3918405/150‑1 (MQ=60)
                                                                                         AATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATAT                                                         >  SRR19973083.3199165/1‑150 (MQ=60)
                                                                                         AATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATAT                                                         <  SRR19973083.2921194/150‑1 (MQ=60)
                                                                                           TGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGT                                                       <  SRR19973083.1127892/150‑1 (MQ=60)
                                                                                             TTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTAC                                                     <  SRR19973083.3451387/150‑1 (MQ=60)
                                                                                              TCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACG                                                    <  SRR19973083.5097928/150‑1 (MQ=60)
                                                                                              TCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACG                                                    <  SRR19973083.3169142/150‑1 (MQ=60)
                                                                                               CAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGT                                                   <  SRR19973083.660752/150‑1 (MQ=60)
                                                                                               CAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGT                                                   <  SRR19973083.660865/150‑1 (MQ=60)
                                                                                                AAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTA                                                  >  SRR19973083.4537023/1‑150 (MQ=60)
                                                                                                  ATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGC                                                <  SRR19973083.2559747/150‑1 (MQ=60)
                                                                                                  ATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGC                                                <  SRR19973083.548196/150‑1 (MQ=60)
                                                                                                   TCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCC                                               <  SRR19973083.4627527/150‑1 (MQ=60)
                                                                                                     GAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCT                                             <  SRR19973083.4580273/150‑1 (MQ=60)
                                                                                                      AGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTA                                            >  SRR19973083.1817937/1‑150 (MQ=60)
                                                                                                      AGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTA                                            >  SRR19973083.1818631/1‑150 (MQ=60)
                                                                                                       GGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTAT                                           >  SRR19973083.4620189/1‑150 (MQ=60)
                                                                                                         GGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCG                                         <  SRR19973083.4591905/150‑1 (MQ=60)
                                                                                                         GGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCG                                         <  SRR19973083.1476145/150‑1 (MQ=60)
                                                                                                          GATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGC                                        >  SRR19973083.4832010/1‑150 (MQ=60)
                                                                                                          GATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGC                                        >  SRR19973083.814476/1‑150 (MQ=60)
                                                                                                             TTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACA                                     <  SRR19973083.2371330/150‑1 (MQ=60)
                                                                                                              TCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACAT                                    <  SRR19973083.398466/150‑1 (MQ=60)
                                                                                                              TCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACAT                                    <  SRR19973083.9363/150‑1 (MQ=60)
                                                                                                                  TGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTT                                >  SRR19973083.4742471/1‑150 (MQ=60)
                                                                                                                    GNAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTAT                              >  SRR19973083.3530081/1‑150 (MQ=60)
                                                                                                                    GAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTAT                              <  SRR19973083.1229512/150‑1 (MQ=60)
                                                                                                                       GCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCC                           <  SRR19973083.4310126/150‑1 (MQ=60)
                                                                                                                           GGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAA                       >  SRR19973083.2648902/1‑150 (MQ=60)
                                                                                                                                  AACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAA                >  SRR19973083.1629645/1‑150 (MQ=60)
                                                                                                                                    CCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATG              >  SRR19973083.1375040/1‑150 (MQ=60)
                                                                                                                                       CTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATG           <  SRR19973083.1865566/150‑1 (MQ=60)
                                                                                                                                        TACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATTCTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGA          <  SRR19973083.2588663/150‑1 (MQ=60)
                                                                                                                                           GGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGAACA       <  SRR19973083.2836922/150‑1 (MQ=60)
                                                                                                                                               GAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCAGGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGAACAAGCC   >  SRR19973083.2646902/1‑150 (MQ=60)
                                                                                                                                                AATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGAACAAGCCA  <  SRR19973083.3054919/150‑1 (MQ=60)
                                                                                                                                                AATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGAACAAGCCA  <  SRR19973083.2777132/150‑1 (MQ=60)
                                                                                                                                                   |                                                                                                                                                  
GTCATCACACTTCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGAACAAGCCA  >  CP009273/2993552‑2993845

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑