Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F86 I0 R1 593 297.4 9515568 94.4% 8982696 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,993,699 C→A 88.9% intergenic (‑449/‑5) ygeR ← / → xdhA LysM domain‑containing M23 family putative peptidase; septation lipoprotein/xanthine dehydrogenase, molybdenum binding subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,993,6990CA88.9% 615.9 / 64.8 210intergenic (‑449/‑5)ygeR/xdhALysM domain‑containing M23 family putative peptidase; septation lipoprotein/xanthine dehydrogenase, molybdenum binding subunit
Reads supporting (aligned to +/- strand):  ref base C (10/14);  new base A (101/85);  total (111/99)
Fisher's exact test for biased strand distribution p-value = 2.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 225 total aligned reads displayed.

CGTCATCACACTTCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGAACAAGCCAG  >  CP009273/2993551‑2993846
                                                                                                                                                    |                                                                                                                                                   
cGTCATCACACTTCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATca                                                                                                                                                    <  1:4590984/150‑1 (MQ=255)
     tcaCACTTCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATaatgcat                                                                                                                                               <  1:1665431/150‑1 (MQ=255)
      cacaCTTCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTTCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCGATGGAAGCGCGGGCAGCAACCGCTACGGGTGAATtatgcatg                                                                                                                                              >  2:4341937/1‑150 (MQ=255)
      cacaCTTCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAATAACCAAATGTTCAAATAGAGGGGATTTGTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATaatgcatg                                                                                                                                              >  1:1546058/1‑150 (MQ=255)
      cacaCTTCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAAACTTGCCTGCTTAGACTAAATCTTTGCCATAAGAAACAAATGTTCAAATCGAGGGGATTTCGATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATaatgcatg                                                                                                                                              >  2:823308/1‑150 (MQ=255)
       acacTTCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTTCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATTTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAatgcatgc                                                                                                                                             >  2:1646295/1‑150 (MQ=255)
          cTTCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGcgcg                                                                                                                                          >  1:2769093/1‑150 (MQ=255)
           ttCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGGAGCAACCGCTACGGGTGAATAATGCATGcgcgg                                                                                                                                         >  2:1839906/1‑149 (MQ=255)
             ccTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTTCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCg                                                                                                                                       >  2:1142172/1‑150 (MQ=255)
              cTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGa                                                                                                                                      >  2:1800215/1‑150 (MQ=255)
               tACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGAt                                                                                                                                     <  2:267311/150‑1 (MQ=255)
                aCCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATg                                                                                                                                    <  2:399463/150‑1 (MQ=255)
                 ccTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGc                                                                                                                                   <  1:2264085/150‑1 (MQ=255)
                     cccTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATAGTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAAACGCTACGGGTGAATAATGCATGCGCGTCGATGCCAtt                                                                                                                               >  2:2058564/1‑150 (MQ=255)
                      ccTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTg                                                                                                                              <  2:3280724/150‑1 (MQ=255)
                         aaCGCTTATCGTCGTTTCTGGCGTAAATATTTCCTGCTTAGACTAAATCTTTGCCATAAGAAACAAATGTTCAAATCGAGGGGCTTTCTATGGAAGCGCGGGAAGCAACCGCGACGGGTGAATAATTCATGCGCGTCGATGCCATTGCTa                                                                                                                           >  1:716465/1‑150 (MQ=255)
                          aCGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTaa                                                                                                                          <  1:3524591/150‑1 (MQ=255)
                           cGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAAACAAATGTTCAAATCGAGGGGAGTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAg                                                                                                                         >  2:4703296/1‑150 (MQ=255)
                             cTTATCGTCGTGTCAGGCGTAAATAATTCATGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTAAAATCGAGGGGAATTCTATGTAAGCGCGGGAATCAACCGCTACGGGAGAAAAATGCATGCGCGTAGCTGCCCATGCTAAGGt                                                                                                                       >  2:3551104/1‑150 (MQ=255)
                                   gtcgtTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACcgg                                                                                                                 >  2:1587584/1‑150 (MQ=255)
                                   gtcgtTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACcgg                                                                                                                 <  1:538961/150‑1 (MQ=255)
                                    tcgtTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACcggg                                                                                                                <  2:281678/150‑1 (MQ=255)
                                       tttCTGGCGTAAATCTTGCCTGCTTAGACTAACTCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACcgggcgg                                                                                                             <  2:4416665/150‑1 (MQ=255)
                                        ttCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACcgggcggg                                                                                                            >  1:3144053/1‑150 (MQ=255)
                                        ttCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACcgggcggg                                                                                                            >  2:1344455/1‑150 (MQ=255)
                                           tGGCGTAAATCTTGCCTGCTTAGACTAACTCTTTGCCATAAGAACCAAATGGTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCAc                                                                                                         <  2:2054967/150‑1 (MQ=255)
                                           tGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCAc                                                                                                         >  1:1235877/1‑150 (MQ=255)
                                           tGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCAc                                                                                                         >  1:1234923/1‑150 (MQ=255)
                                              cGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGat                                                                                                      >  2:4569585/1‑150 (MQ=255)
                                              cGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGat                                                                                                      <  1:2435486/150‑1 (MQ=255)
                                              cGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGat                                                                                                      >  2:1403454/1‑150 (MQ=255)
                                               gTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGata                                                                                                     >  1:1776770/1‑150 (MQ=255)
                                                 aaaTCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGAtata                                                                                                   <  1:2571771/150‑1 (MQ=255)
                                                 aaaTCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGAtata                                                                                                   <  1:1903381/150‑1 (MQ=255)
                                                 aaaTCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGAtata                                                                                                   >  1:456546/1‑150 (MQ=255)
                                                   aTCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACt                                                                                                 <  1:151054/150‑1 (MQ=255)
                                                     cTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGa                                                                                               >  2:2840857/1‑150 (MQ=255)
                                                           tGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGAtta                                                                                         <  1:3985146/150‑1 (MQ=255)
                                                            gCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGAttat                                                                                        >  1:2052700/1‑150 (MQ=255)
                                                             cTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGAttatg                                                                                       >  2:384912/1‑150 (MQ=255)
                                                               tAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGAttatgtt                                                                                     <  1:1594868/150‑1 (MQ=255)
                                                               tAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGAttatgtt                                                                                     >  1:613629/1‑150 (MQ=255)
                                                               tAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGAttatgtt                                                                                     >  1:1615924/1‑150 (MQ=255)
                                                                 gACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGAttatgttat                                                                                   >  2:1384551/1‑150 (MQ=255)
                                                                    tAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGc                                                                                >  2:931804/1‑150 (MQ=255)
                                                                     aaaTCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCg                                                                               >  2:987345/1‑150 (MQ=255)
                                                                     aaaTCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCg                                                                               >  1:960089/1‑150 (MQ=255)
                                                                      aaTCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCgg                                                                              >  1:3359917/1‑150 (MQ=255)
                                                                          tttGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCAt                                                                          <  2:4086521/150‑1 (MQ=255)
                                                                           ttGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCAtg                                                                         <  1:118279/150‑1 (MQ=255)
                                                                               cATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGtt                                                                     >  1:3049313/1‑150 (MQ=255)
                                                                                aTAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTa                                                                    <  2:2902190/150‑1 (MQ=255)
                                                                                aTAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTa                                                                    >  2:2774784/1‑150 (MQ=255)
                                                                                  aaGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATg                                                                  <  1:1516001/150‑1 (MQ=255)
                                                                                    gAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCg                                                                <  2:4627416/150‑1 (MQ=255)
                                                                                      aCCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGaa                                                              <  1:1859050/150‑1 (MQ=255)
                                                                                          aaTGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAatat                                                          >  1:3320030/1‑150 (MQ=255)
                                                                                           aTGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATg                                                         >  1:179187/1‑150 (MQ=255)
                                                                                           aTGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATg                                                         <  2:1536114/150‑1 (MQ=255)
                                                                                             gTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTa                                                       <  2:638331/150‑1 (MQ=255)
                                                                                             gTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTa                                                       >  1:1837232/1‑150 (MQ=255)
                                                                                               tCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACg                                                     >  1:1089622/1‑150 (MQ=255)
                                                                                                  aaTCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAg                                                  >  2:1487205/1‑150 (MQ=255)
                                                                                                  aaTCGAGGGGAGTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAg                                                  <  2:2560825/150‑1 (MQ=255)
                                                                                                    tCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGcc                                                <  2:4123457/150‑1 (MQ=255)
                                                                                                      gAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCt                                              <  2:2312874/150‑1 (MQ=255)
                                                                                                      gAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCt                                              <  2:1005984/150‑1 (MQ=255)
                                                                                                            aTTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGca                                        >  2:720431/1‑150 (MQ=255)
                                                                                                             tttCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGcac                                       <  1:3334246/150‑1 (MQ=255)
                                                                                                                cTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATg                                    <  2:574346/150‑1 (MQ=255)
                                                                                                                   tGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGtt                                 >  1:3230617/1‑150 (MQ=255)
                                                                                                                   tGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGtt                                 <  2:630797/150‑1 (MQ=255)
                                                                                                                   tGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGtt                                 <  2:3001570/150‑1 (MQ=255)
                                                                                                                     gAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTAt                               <  2:3226943/150‑1 (MQ=255)
                                                                                                                      aaGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATg                              >  2:1921057/1‑150 (MQ=255)
                                                                                                                            gggAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTaa                        <  2:1859281/150‑1 (MQ=255)
                                                                                                                            gggAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTCTCGCACATGGGTATGCCGTaa                        >  1:556698/1‑150 (MQ=255)
                                                                                                                             ggAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAg                       <  1:4535125/150‑1 (MQ=255)
                                                                                                                             ggAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAg                       >  2:2600586/1‑150 (MQ=255)
                                                                                                                                 gCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTAtt                   <  2:3408863/150‑1 (MQ=255)
                                                                                                                                 gCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTAtt                   >  1:3888818/1‑150 (MQ=255)
                                                                                                                                  cAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTa                  >  2:3702824/1‑150 (MQ=255)
                                                                                                                                   aaCCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTaa                 <  1:1646669/150‑1 (MQ=255)
                                                                                                                                   aaCCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTaa                 >  2:3778109/1‑150 (MQ=255)
                                                                                                                                   aaCCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTaa                 >  2:869100/1‑150 (MQ=255)
                                                                                                                                   aaCCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTaa                 >  2:330087/1‑150 (MQ=255)
                                                                                                                                   aaCCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTaa                 >  2:129809/1‑150 (MQ=255)
                                                                                                                                    aCCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAat                >  1:262534/1‑150 (MQ=255)
                                                                                                                                     ccGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAatg               <  1:3096220/150‑1 (MQ=255)
                                                                                                                                       gCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAatgat             <  2:3049377/150‑1 (MQ=255)
                                                                                                                                       gCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAatgat             <  2:3560302/150‑1 (MQ=255)
                                                                                                                                        cTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAatgatg            <  2:2358600/150‑1 (MQ=255)
                                                                                                                                         tACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAAtgatga           >  1:940182/1‑150 (MQ=255)
                                                                                                                                         tACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAAtgatga           >  1:1395682/1‑150 (MQ=255)
                                                                                                                                          aCGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGaa          <  1:2525612/150‑1 (MQ=255)
                                                                                                                                           cGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGAAc         >  1:3224332/1‑150 (MQ=255)
                                                                                                                                           cGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGAAc         >  2:298271/1‑150 (MQ=255)
                                                                                                                                              gTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGGTGAACAAg      <  2:2173141/150‑1 (MQ=255)
                                                                                                                                              gTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGAACAAg      >  1:4491293/1‑150 (MQ=255)
                                                                                                                                                 aataatGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGAACAAGCCa   <  1:1840734/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CGTCATCACACTTCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGAACAAGCCAG  >  CP009273/2993551‑2993846

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGTCATCACACTTCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGAACAAGCCAG  >  CP009273/2993551‑2993846
                                                                                                                                                    |                                                                                                                                                   
CGTCATCACACTTCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCA                                                                                                                                                    <  SRR19973082.4618855/150‑1 (MQ=60)
CGTCATCACACTTCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAA                                                                                                                                                    <  SRR19973082.3741438/150‑1 (MQ=60)
CGTCATCACACTTCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTAAAATAGAGGGGATTTCTATGGTAGCGCGGGAAGCAACCGCTACGGGTGAATAA                                                                                                                                                    >  SRR19973082.1546250/1‑150 (MQ=60)
   CATCACACTTCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGC                                                                                                                                                 <  SRR19973082.2410713/150‑1 (MQ=60)
    ATCACACTTCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCA                                                                                                                                                <  SRR19973082.1801987/150‑1 (MQ=60)
     TCACACTTCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCAT                                                                                                                                               <  SRR19973082.1676553/150‑1 (MQ=60)
     TCACACTTCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCAT                                                                                                                                               <  SRR19973082.1676517/150‑1 (MQ=60)
      CACACTTCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAATAACCAAATGTTCAAATAGAGGGGATTTGTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATG                                                                                                                                              >  SRR19973082.1556222/1‑150 (MQ=60)
       ACACTTCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATAGTGCCTGCGTAGACTAAATATTTGCCATAAGAACCAAATGTTCAAATAGAGGGGATTTGTATGGAAGCGCGGGAAGCAAACGCTACGGGTGAATAATGCCTGC                                                                                                                                             >  SRR19973082.1398353/1‑150 (MQ=60)
          CTTCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCG                                                                                                                                          >  SRR19973082.2785807/1‑150 (MQ=60)
                ACCTCCCCTAACGCTTATCGTCGTTTGTGGCGTAAATATTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATAGAGGGGATTTGTATTGTAGCGCGGGGAGCAACCGCTACGGGTGAATAATGACTGCGCGTCGATG                                                                                                                                    >  SRR19973082.4152450/1‑150 (MQ=60)
                 CCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGC                                                                                                                                   <  SRR19973082.2278278/150‑1 (MQ=60)
                  CTCCCCTAACGCTTATCGTCGTTTATGGCGTAAATATTGCCTGCTTAGACTAAAAATTTTCCAAAATAACCAAATGTTCAAAACGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCC                                                                                                                                  >  SRR19973082.1169008/1‑150 (MQ=60)
                         AACGCTTATCGTCGTTTCTGGCGTAAATATTTCCTGCTTAGACTAAATCTTTGCCATAAGAAACAAATGTTCAAATCGAGGGGCTTTCTATGGAAGCGCGGGAAGCAACCGCGACGGGTGAATAATTCATGCGCGTCGATGCCATTGCTA                                                                                                                           >  SRR19973082.721259/1‑150 (MQ=60)
                         AACGCTTATCGTCGTTTATGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATTCCATTTCTA                                                                                                                           >  SRR19973082.3581884/1‑150 (MQ=60)
                          ACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAA                                                                                                                          <  SRR19973082.3546069/150‑1 (MQ=60)
                                  CGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCG                                                                                                                  <  SRR19973082.1992680/150‑1 (MQ=60)
                                   GTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGG                                                                                                                 >  SRR19973082.578443/1‑150 (MQ=60)
                                   GTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGG                                                                                                                 <  SRR19973082.542834/150‑1 (MQ=60)
                                   GTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGG                                                                                                                 <  SRR19973082.544058/150‑1 (MQ=60)
                                     CGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGC                                                                                                               >  SRR19973082.3395529/1‑150 (MQ=60)
                                        TTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGG                                                                                                            >  SRR19973082.3163069/1‑150 (MQ=60)
                                           TGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCAC                                                                                                         <  SRR19973082.4755606/150‑1 (MQ=60)
                                           TGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCAC                                                                                                         >  SRR19973082.1243373/1‑150 (MQ=60)
                                           TGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCAC                                                                                                         >  SRR19973082.1244253/1‑150 (MQ=60)
                                           TGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCAC                                                                                                         >  SRR19973082.1244339/1‑150 (MQ=60)
                                             GCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGA                                                                                                       >  SRR19973082.1837270/1‑150 (MQ=60)
                                              CGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGAT                                                                                                      <  SRR19973082.2450632/150‑1 (MQ=60)
                                               GTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATA                                                                                                     >  SRR19973082.1788490/1‑150 (MQ=60)
                                                TAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATAT                                                                                                    <  SRR19973082.937274/150‑1 (MQ=60)
                                                 AAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATA                                                                                                   <  SRR19973082.2587789/150‑1 (MQ=60)
                                                 AAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATA                                                                                                   <  SRR19973082.2587796/150‑1 (MQ=60)
                                                 AAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATA                                                                                                   <  SRR19973082.1915562/150‑1 (MQ=60)
                                                 AAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATA                                                                                                   <  SRR19973082.1914730/150‑1 (MQ=60)
                                                 AAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATA                                                                                                   >  SRR19973082.459736/1‑150 (MQ=60)
                                                 AAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATA                                                                                                   >  SRR19973082.459589/1‑150 (MQ=60)
                                                   ATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACT                                                                                                 <  SRR19973082.152394/150‑1 (MQ=60)
                                                           TGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTA                                                                                         <  SRR19973082.4009415/150‑1 (MQ=60)
                                                            GCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTAT                                                                                        >  SRR19973082.2065758/1‑150 (MQ=60)
                                                               TAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCTCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTT                                                                                     <  SRR19973082.1605179/150‑1 (MQ=60)
                                                               TAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTT                                                                                     <  SRR19973082.1605364/150‑1 (MQ=60)
                                                               TAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTT                                                                                     >  SRR19973082.1626598/1‑150 (MQ=60)
                                                               TAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTT                                                                                     >  SRR19973082.2378157/1‑150 (MQ=60)
                                                               TAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTT                                                                                     >  SRR19973082.617968/1‑150 (MQ=60)
                                                                     AAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCG                                                                               >  SRR19973082.320513/1‑150 (MQ=60)
                                                                     AAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCG                                                                               <  SRR19973082.4724391/150‑1 (MQ=60)
                                                                     AAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCG                                                                               >  SRR19973082.966767/1‑150 (MQ=60)
                                                                     AAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCG                                                                               >  SRR19973082.966774/1‑150 (MQ=60)
                                                                      AATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGG                                                                              >  SRR19973082.3380373/1‑150 (MQ=60)
                                                                      AATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGG                                                                              >  SRR19973082.3380689/1‑150 (MQ=60)
                                                                      AATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGG                                                                              >  SRR19973082.3380694/1‑150 (MQ=60)
                                                                       ATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGG                                                                             >  SRR19973082.319100/1‑150 (MQ=60)
                                                                       ATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGG                                                                             >  SRR19973082.2706365/1‑150 (MQ=60)
                                                                          TTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCAT                                                                          <  SRR19973082.4248285/150‑1 (MQ=60)
                                                                           TTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATG                                                                         <  SRR19973082.119377/150‑1 (MQ=60)
                                                                            TGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGT                                                                        >  SRR19973082.1238944/1‑150 (MQ=60)
                                                                               CATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTT                                                                     >  SRR19973082.3068025/1‑150 (MQ=60)
                                                                               CATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTT                                                                     >  SRR19973082.3068089/1‑150 (MQ=60)
                                                                                ATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTA                                                                    >  SRR19973082.4550467/1‑150 (MQ=60)
                                                                                  AAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATG                                                                  <  SRR19973082.1526071/150‑1 (MQ=60)
                                                                                      ACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAA                                                              <  SRR19973082.1871101/150‑1 (MQ=60)
                                                                                      ACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAA                                                              <  SRR19973082.1869901/150‑1 (MQ=60)
                                                                                      ACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAA                                                              >  SRR19973082.717997/1‑150 (MQ=60)
                                                                                      ACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAA                                                              <  SRR19973082.1869880/150‑1 (MQ=60)
                                                                                         AAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATA                                                           <  SRR19973082.1664856/150‑1 (MQ=60)
                                                                                          AATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATAT                                                          <  SRR19973082.4016849/150‑1 (MQ=60)
                                                                                          AATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATAT                                                          <  SRR19973082.2119052/150‑1 (MQ=60)
                                                                                          AATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATAT                                                          >  SRR19973082.3340559/1‑150 (MQ=60)
                                                                                           ATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATG                                                         >  SRR19973082.2572113/1‑150 (MQ=60)
                                                                                           ATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATG                                                         >  SRR19973082.180708/1‑150 (MQ=60)
                                                                                            TGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGT                                                        >  SRR19973082.13912/1‑150 (MQ=60)
                                                                                             GTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTA                                                       >  SRR19973082.1849211/1‑150 (MQ=60)
                                                                                               TCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACG                                                     >  SRR19973082.1097024/1‑150 (MQ=60)
                                                                                               TCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACG                                                     <  SRR19973082.4607280/150‑1 (MQ=60)
                                                                                                    TCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCC                                                >  SRR19973082.788970/1‑150 (MQ=60)
                                                                                                        GGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTAT                                            >  SRR19973082.3964769/1‑150 (MQ=60)
                                                                                                             TTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCAC                                       <  SRR19973082.3352265/150‑1 (MQ=60)
                                                                                                             TTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCAC                                       <  SRR19973082.3354869/150‑1 (MQ=60)
                                                                                                                 TATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGG                                   >  SRR19973082.950535/1‑150 (MQ=60)
                                                                                                                   TGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTT                                 >  SRR19973082.3250402/1‑150 (MQ=60)
                                                                                                                   TGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTT                                 >  SRR19973082.3250408/1‑150 (MQ=60)
                                                                                                                            GGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTCTCGCACATGGGTATGCCGTAA                        >  SRR19973082.560677/1‑150 (MQ=60)
                                                                                                                            GGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAA                        <  SRR19973082.1538407/150‑1 (MQ=60)
                                                                                                                            GGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAA                        >  SRR19973082.156259/1‑150 (MQ=60)
                                                                                                                             GGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAG                       <  SRR19973082.4562693/150‑1 (MQ=60)
                                                                                                                                AGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTAT                    <  SRR19973082.1524841/150‑1 (MQ=60)
                                                                                                                                 GCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATT                   >  SRR19973082.3912621/1‑150 (MQ=60)
                                                                                                                                   AACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAA                 <  SRR19973082.1657248/150‑1 (MQ=60)
                                                                                                                                   AACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAA                 <  SRR19973082.1657626/150‑1 (MQ=60)
                                                                                                                                    ACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAAT                >  SRR19973082.264514/1‑150 (MQ=60)
                                                                                                                                     CCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATG               <  SRR19973082.1748713/150‑1 (MQ=60)
                                                                                                                                     CCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATG               <  SRR19973082.3115106/150‑1 (MQ=60)
                                                                                                                                     CCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATG               <  SRR19973082.3115324/150‑1 (MQ=60)
                                                                                                                                         TACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGA           >  SRR19973082.946707/1‑150 (MQ=60)
                                                                                                                                         TACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGA           >  SRR19973082.1405246/1‑150 (MQ=60)
                                                                                                                                          ACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGAA          <  SRR19973082.2541379/150‑1 (MQ=60)
                                                                                                                                          ACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGAA          <  SRR19973082.2541295/150‑1 (MQ=60)
                                                                                                                                           CGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGAAC         >  SRR19973082.3244070/1‑150 (MQ=60)
                                                                                                                                            GGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGAACA        <  SRR19973082.1608717/150‑1 (MQ=60)
                                                                                                                                            GGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGAACA        <  SRR19973082.3019774/150‑1 (MQ=60)
                                                                                                                                             GGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGAACAA       <  SRR19973082.1500204/150‑1 (MQ=60)
                                                                                                                                              GTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGAACAAG      >  SRR19973082.4518583/1‑150 (MQ=60)
                                                                                                                                                 AATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGAACAAGCCA   >  SRR19973082.2765909/1‑150 (MQ=60)
                                                                                                                                                 AATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGAACAAGCCA   <  SRR19973082.1852726/150‑1 (MQ=60)
                                                                                                                                                 AATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGAACAAGCCA   <  SRR19973082.1851897/150‑1 (MQ=60)
                                                                                                                                                  ATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATCAATGATGAACAAGCCAG  >  SRR19973082.3303267/1‑150 (MQ=60)
                                                                                                                                                  ATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGAACAAGCCAG  <  SRR19973082.4731632/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                   
CGTCATCACACTTCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGAACAAGCCAG  >  CP009273/2993551‑2993846

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑