Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F142 I0 R1 859 274.6 8723680 96.4% 8409627 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,993,699 C→A 100% intergenic (‑449/‑5) ygeR ← / → xdhA LysM domain‑containing M23 family putative peptidase; septation lipoprotein/xanthine dehydrogenase, molybdenum binding subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,993,6990CA96.7% 840.4 / 15.4 246intergenic (‑449/‑5)ygeR/xdhALysM domain‑containing M23 family putative peptidase; septation lipoprotein/xanthine dehydrogenase, molybdenum binding subunit
Reads supporting (aligned to +/- strand):  ref base C (2/6);  new base A (118/120);  total (120/126)
Fisher's exact test for biased strand distribution p-value = 2.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.32e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 253 total aligned reads displayed.

CGTCATCACACTTCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGAACAAGCCAGAA  >  CP009273/2993551‑2993848
                                                                                                                                                    |                                                                                                                                                     
cGTCATCACACTTCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAATAACCAAATGTTCAAATCGAGGGGGTTTCTATTGAAGCGCGGGGAGCAACCGCTACGGGGGAATca                                                                                                                                                      >  1:1019242/1‑150 (MQ=255)
      cacaCTTCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGGTTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATaatgcatg                                                                                                                                                >  1:1412272/1‑150 (MQ=255)
       acacTTCCTACCTCCCCTAACGCTTATCGTCGTGTGTGGCGTAAATATTGCCGGCTTAGACTAAATCTTTGCCATAAGAACCCAATGTTGAAATCGAGGGGATTTATATGGAAGCGCGGGTAGCAACCGCGACGGGGGCATAatgcatgc                                                                                                                                               >  1:2264480/1‑150 (MQ=255)
        cacTTCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATgcg                                                                                                                                              <  2:1400655/150‑1 (MQ=255)
        cacTTCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCGGCGTAGACTAAAACTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATgcg                                                                                                                                              >  1:1398893/1‑150 (MQ=255)
         acTTCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTTCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATgcgc                                                                                                                                             >  2:172736/1‑150 (MQ=255)
         acTTCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCGTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATAGAGGGGATTTCGATGGAAGCGCGGGCAGCAAACGCTACGGGTGAATAATGCATgcgc                                                                                                                                             >  1:2011856/1‑150 (MQ=255)
            tCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAATAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATTCGCGTc                                                                                                                                          >  2:1556986/1‑150 (MQ=255)
            tCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTc                                                                                                                                          >  2:4359418/1‑150 (MQ=255)
             ccTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCg                                                                                                                                         <  2:953699/150‑1 (MQ=255)
             ccTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCg                                                                                                                                         <  1:4102847/150‑1 (MQ=255)
                aCCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATg                                                                                                                                      <  2:1650259/150‑1 (MQ=255)
                    ccccTAACGCTTATCGTCGTTTCTGGCGTAAATATTTCCTGCTTAGACTAAATATTTGCCATAAGAACCAAATGTTAAAAAAGAGGGGAATTCGATTGAAGCGCGGGAAGCAACCGCAACGGGTGAATAATGCATGCGCGGCGATGCCag                                                                                                                                  >  1:148888/1‑149 (MQ=255)
                     cccTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCAtt                                                                                                                                 <  1:1737076/150‑1 (MQ=255)
                         aaCGCTTATCGTCGTTTCTGGCGTAAATATTGCCTGCTTAGACTAAATCTTTTCCATAAGAACCAAATTTTCAAATAGAGGGGATTTATATGGAAGCGCGGGAAGCAAACGCTACGGGTGAAAAATGCATGCGCGTCGATGcccatgcga                                                                                                                             >  1:3905094/1‑143 (MQ=255)
                             cTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGt                                                                                                                         <  1:2726967/150‑1 (MQ=255)
                              ttATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTc                                                                                                                        <  2:3348357/150‑1 (MQ=255)
                                 tcgtcgTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCAcc                                                                                                                     <  1:1040773/150‑1 (MQ=255)
                                   gtcgtTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACcgg                                                                                                                   >  2:642283/1‑150 (MQ=255)
                                     cgtTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACcgggc                                                                                                                 >  2:4176207/1‑150 (MQ=255)
                                      gtTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACcgggcg                                                                                                                <  2:2194506/150‑1 (MQ=255)
                                         tCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCgggcgggc                                                                                                             <  1:4338527/150‑1 (MQ=255)
                                          cTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCa                                                                                                            <  1:1013558/150‑1 (MQ=255)
                                           tGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGACGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCAc                                                                                                           >  1:2419377/1‑150 (MQ=255)
                                              cGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGat                                                                                                        >  2:3457481/1‑150 (MQ=255)
                                               gTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGata                                                                                                       <  2:553236/150‑1 (MQ=255)
                                                tAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCCCGatat                                                                                                      >  1:1834821/1‑150 (MQ=255)
                                                  aaTCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATAc                                                                                                    >  2:2667402/1‑150 (MQ=255)
                                                    tCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTg                                                                                                  <  2:2574605/150‑1 (MQ=255)
                                                    tCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTg                                                                                                  <  1:1004058/150‑1 (MQ=255)
                                                      ttGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGAc                                                                                                <  1:4259879/150‑1 (MQ=255)
                                                      ttGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGAc                                                                                                >  2:373296/1‑150 (MQ=255)
                                                       tGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACg                                                                                               <  2:4333478/150‑1 (MQ=255)
                                                       tGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACg                                                                                               <  2:2996747/150‑1 (MQ=255)
                                                           tGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGAtta                                                                                           >  2:3149785/1‑150 (MQ=255)
                                                           tGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGAtta                                                                                           <  1:2308627/150‑1 (MQ=255)
                                                             cTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGAttatg                                                                                         >  2:2706009/1‑150 (MQ=255)
                                                             cTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGAttagg                                                                                         >  2:531422/1‑148 (MQ=255)
                                                               tAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGAttctgtt                                                                                       >  2:3053488/1‑150 (MQ=255)
                                                                 gACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGAttatgttat                                                                                     <  1:4199213/150‑1 (MQ=255)
                                                                    tAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGc                                                                                  >  1:3864118/1‑150 (MQ=255)
                                                                     aaaTCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGAGTATGTTATGGCg                                                                                 >  1:378772/1‑150 (MQ=255)
                                                                      aaTCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCgg                                                                                >  1:2528188/1‑150 (MQ=255)
                                                                      aaTCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCgg                                                                                >  1:42522/1‑150 (MQ=255)
                                                                      aaTCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCgg                                                                                >  1:3995881/1‑150 (MQ=255)
                                                                       aTCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCggg                                                                               >  2:26452/1‑150 (MQ=255)
                                                                          tttGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCAt                                                                            <  1:2233989/150‑1 (MQ=255)
                                                                          tttGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCAt                                                                            >  1:1569319/1‑150 (MQ=255)
                                                                               cATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATTGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGtt                                                                       <  2:3901573/150‑1 (MQ=255)
                                                                               cATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGtt                                                                       >  1:337807/1‑150 (MQ=255)
                                                                                  aaGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATg                                                                    <  2:2509761/150‑1 (MQ=255)
                                                                                   aGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGc                                                                   >  2:3317620/1‑150 (MQ=255)
                                                                                   aGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGc                                                                   >  2:1118171/1‑150 (MQ=255)
                                                                                   aGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGc                                                                   >  2:213865/1‑150 (MQ=255)
                                                                                   aGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGc                                                                   >  2:3958355/1‑150 (MQ=255)
                                                                                       ccAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGaaa                                                               >  2:3455086/1‑150 (MQ=255)
                                                                                        cAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAat                                                              <  2:367617/150‑1 (MQ=255)
                                                                                          aaTGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAatat                                                            <  1:2212798/150‑1 (MQ=255)
                                                                                          aaTGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAatat                                                            >  1:3506724/1‑150 (MQ=255)
                                                                                             gTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTa                                                         >  1:3157429/1‑150 (MQ=255)
                                                                                             gTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTa                                                         >  1:967873/1‑150 (MQ=255)
                                                                                              ttCAAATCGAGGGGATTTCTATGGAAGCTCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTAc                                                        <  2:2813756/150‑1 (MQ=255)
                                                                                               tCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACg                                                       <  2:1046907/150‑1 (MQ=255)
                                                                                                cAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGt                                                      >  1:4276304/1‑150 (MQ=255)
                                                                                                  aaTCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAg                                                    >  2:798143/1‑150 (MQ=255)
                                                                                                   aTCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGc                                                   >  1:3319300/1‑150 (MQ=255)
                                                                                                   aTCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGc                                                   >  1:1940790/1‑150 (MQ=255)
                                                                                                    tCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGcc                                                  <  2:708286/150‑1 (MQ=255)
                                                                                                    tCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGcc                                                  <  1:1959854/150‑1 (MQ=255)
                                                                                                    tCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGcc                                                  <  2:710386/150‑1 (MQ=255)
                                                                                                      gAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCt                                                >  2:632270/1‑150 (MQ=255)
                                                                                                      gAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCt                                                >  1:1929346/1‑150 (MQ=255)
                                                                                                       aGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTa                                               >  2:188733/1‑150 (MQ=255)
                                                                                                        ggggATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTAt                                              >  2:2021834/1‑150 (MQ=255)
                                                                                                          ggATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCg                                            >  1:543552/1‑150 (MQ=255)
                                                                                                          ggATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCg                                            >  1:2957109/1‑150 (MQ=255)
                                                                                                             tttCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGcac                                         <  1:3704418/150‑1 (MQ=255)
                                                                                                               tCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACAt                                       <  1:3205373/150‑1 (MQ=255)
                                                                                                                    ggAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTCAGGTCACCGGGCGGGCACGATATACTGACGATTAGGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTa                                  >  1:3286569/1‑150 (MQ=255)
                                                                                                                    ggAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTa                                  <  2:2351499/150‑1 (MQ=255)
                                                                                                                     gAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTAt                                 <  1:3341782/150‑1 (MQ=255)
                                                                                                                     gAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTAt                                 <  1:530172/150‑1 (MQ=255)
                                                                                                                     gAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTAt                                 <  2:1243980/150‑1 (MQ=255)
                                                                                                                      aaGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATg                                <  2:426615/150‑1 (MQ=255)
                                                                                                                       aGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGc                               >  1:1204522/1‑150 (MQ=255)
                                                                                                                            gggAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTaa                          >  1:2259887/1‑150 (MQ=255)
                                                                                                                              gAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGt                        >  1:1238950/1‑150 (MQ=255)
                                                                                                                              gAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGt                        <  1:850326/150‑1 (MQ=255)
                                                                                                                              gAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGt                        >  2:299158/1‑150 (MQ=255)
                                                                                                                              gAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGt                        <  1:3027236/150‑1 (MQ=255)
                                                                                                                                 gCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTAtt                     <  1:3402409/150‑1 (MQ=255)
                                                                                                                                  cAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTa                    >  2:3512536/1‑150 (MQ=255)
                                                                                                                                     ccGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAatg                 <  1:4359418/150‑1 (MQ=255)
                                                                                                                                         tACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAAtgatga             >  1:3890047/1‑150 (MQ=255)
                                                                                                                                           cGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGAAc           >  1:751553/1‑150 (MQ=255)
                                                                                                                                            gggTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGAACa          <  2:2437575/150‑1 (MQ=255)
                                                                                                                                               tGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGAACAAGc       >  1:34921/1‑150 (MQ=255)
                                                                                                                                                gAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGAACAAGcc      >  2:2803795/1‑150 (MQ=255)
                                                                                                                                                 aataatGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGAACAAGCCa     >  2:824931/1‑150 (MQ=255)
                                                                                                                                                    catgcatgCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGAACAAGCCAGaa  >  1:1715949/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CGTCATCACACTTCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGAACAAGCCAGAA  >  CP009273/2993551‑2993848

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGTCATCACACTTCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGAACAAGCCAGAA  >  CP009273/2993551‑2993848
                                                                                                                                                    |                                                                                                                                                     
CGTCATCACACTTCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAATAACCAAATGTTCAAATCGAGGGGGTTTCTATTGAAGCGCGGGGAGCAACCGCTACGGGGGAATCA                                                                                                                                                      >  SRR19973079.1024945/1‑150 (MQ=60)
      CACACTTCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGGTTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATG                                                                                                                                                >  SRR19973079.1419966/1‑150 (MQ=60)
       ACACTTCCTACCTCCCCTAACGCTTATCGTCGTGTGTGGCGTAAATATTGCCGGCTTAGACTAAATCTTTGCCATAAGAACCCAATGTTGAAATCGAGGGGATTTATATGGAAGCGCGGGTAGCAACCGCGACGGGGGCATAATGCATGC                                                                                                                                               >  SRR19973079.2276232/1‑150 (MQ=60)
       ACACTTCCTACCTCCCCTAACGCTTATCGTCGTGTCTGGCGTAAAAATTTCCTGCGTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTATATGGAAGCGCGGGAAGCAACCGCAACGGGTGAATAATGCATGC                                                                                                                                               >  SRR19973079.2276275/1‑150 (MQ=60)
        CACTTCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCGGCGTAGACTAAAACTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCG                                                                                                                                              >  SRR19973079.1406560/1‑150 (MQ=60)
         ACTTCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCGTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATAGAGGGGATTTCGATGGAAGCGCGGGCAGCAAACGCTACGGGTGAATAATGCATGCGC                                                                                                                                             >  SRR19973079.2022481/1‑150 (MQ=60)
           TTCCTACCTCCCCTAACGCTTATCGTCGTTTGTGGCGTAAATCTTGCCTGCGTAGACTAAATCTTTGCCATAAGAACCAAAATTTCAAATAGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATTCATGCGCGT                                                                                                                                           >  SRR19973079.3132206/1‑150 (MQ=60)
            TCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTC                                                                                                                                          <  SRR19973079.3235666/150‑1 (MQ=60)
             CCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCG                                                                                                                                         <  SRR19973079.4123645/150‑1 (MQ=60)
               TACCTCCCCTAACGCTTATCGTCGTTTCTTGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAATAACCAAATTTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGAT                                                                                                                                       >  SRR19973079.1399257/1‑150 (MQ=60)
               TACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAAAAATTCCAGCTTAGACTAAATATTTTCCAAAAGAACCAAATGTTAAAATAGAGGGGATTTGTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATTCATTCGCGTCGAT                                                                                                                                       >  SRR19973079.1400446/1‑150 (MQ=60)
                ACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCGTAGACTAAATCTTTGCCATAAGAACCAAATGTTAAAATCGAGGGGATTTGTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCAAGCGCGTCGATG                                                                                                                                      >  SRR19973079.1968948/1‑150 (MQ=60)
                   TCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATTCCA                                                                                                                                   >  SRR19973079.4316592/1‑150 (MQ=60)
                   TCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACAAAATATTTGCCATAAGAACCAAATGTTCAAATAGAGGGGATTTGTATGGAAGCGCGGGAAGCAACCCCTACGGGTGAATAATGCATGCGCGTCGCTGCCA                                                                                                                                   >  SRR19973079.1946471/1‑150 (MQ=60)
                    CCCCTAACGCTTATCGTCGTTTCTGGCGTAAATATTTCCTGCTTAGACTAAATATTTGCCATAAGAACCAAATGTTAAAAAAGAGGGGAATTCGATTGAAGCGCGGGAAGCAACCGCAACGGGTGAATAATGCATGCGCGGCGATGCCAG                                                                                                                                  >  SRR19973079.150053/1‑150 (MQ=60)
                     CCCTAACGCTTATCGTCGTTTGTGGCGTAAAAATTGCCGGCGTAGACTAAAACTTTTCCAAAATAAACAAATGTTCAAATAGAGGGGATTTATATGGAAGCGCGGGAAGCAACCCCTACGGGTGAAAAAATCATGCGCGTCGATGCCAAT                                                                                                                                 >  SRR19973079.2999073/1‑150 (MQ=58)
                     CCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATT                                                                                                                                 <  SRR19973079.1746290/150‑1 (MQ=60)
                     CCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCGTAGACTAAATATTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCGACGGGTGAATAATGCATGCGCGTCGATGCCATT                                                                                                                                 >  SRR19973079.1029898/1‑150 (MQ=60)
                         AACGCTTATCGTCGTTTCTGGCGTAAATATTGCCTGCTTAGACTAAATCTTTTCCATAAGAACCAAATTTTCAAATAGAGGGGATTTATATGGAAGCGCGGGAAGCAAACGCTACGGGTGAAAAATGCATGCGCGTCGATGCCcatgcga                                                                                                                             >  SRR19973079.3924892/1‑143 (MQ=60)
                          ACGCTTATCGTCGTTTCTGGCGTAAAACTTTCCAGCTTAGACTAAATCATTGCCCAAAGAAACAAAAGTTAAAATCGAGGGGCTTTCTATTGAAGCGCGGGAAGCAACCGCAAAGGGGGAATAATGCAAGCGCGTCGATGCCAATGCTAA                                                                                                                            >  SRR19973079.3354809/1‑150 (MQ=60)
                           CGCTTATCGTCGTTTCTGGCGTAAAACATTCCTGCTTAGACTAAATATTTTCCAAAATAAACAAAAGTTAAAAAAGAGGGGTTTTCTAAGGAAGCGCGGGAAGCAACCGCAACGGGTGAATAATTCAATCGCGTCGATTCCATTTCTAAG                                                                                                                           >  SRR19973079.1233927/1‑150 (MQ=32)
                             CTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGT                                                                                                                         <  SRR19973079.2741064/150‑1 (MQ=60)
                                 TCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACC                                                                                                                     <  SRR19973079.1046534/150‑1 (MQ=60)
                                   GTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGG                                                                                                                   >  SRR19973079.2250970/1‑150 (MQ=60)
                                      GTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCG                                                                                                                >  SRR19973079.4050122/1‑150 (MQ=60)
                                      GTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCG                                                                                                                <  SRR19973079.4278368/150‑1 (MQ=60)
                                       TTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGG                                                                                                               >  SRR19973079.3134211/1‑150 (MQ=60)
                                         TCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGC                                                                                                             <  SRR19973079.4360304/150‑1 (MQ=60)
                                         TCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGC                                                                                                             <  SRR19973079.4360317/150‑1 (MQ=60)
                                         TCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGC                                                                                                             <  SRR19973079.4360332/150‑1 (MQ=60)
                                          CTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCA                                                                                                            <  SRR19973079.1019249/150‑1 (MQ=60)
                                          CTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCA                                                                                                            <  SRR19973079.1019273/150‑1 (MQ=60)
                                          CTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCA                                                                                                            <  SRR19973079.1019595/150‑1 (MQ=60)
                                           TGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGACGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCAC                                                                                                           >  SRR19973079.2431837/1‑150 (MQ=60)
                                           TGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCAC                                                                                                           <  SRR19973079.1019214/150‑1 (MQ=60)
                                           TGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCAC                                                                                                           <  SRR19973079.3497931/150‑1 (MQ=60)
                                           TGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCAC                                                                                                           <  SRR19973079.2025161/150‑1 (MQ=60)
                                           TGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCAC                                                                                                           >  SRR19973079.3345826/1‑150 (MQ=60)
                                            GGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACG                                                                                                          <  SRR19973079.3546725/150‑1 (MQ=60)
                                               GTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATA                                                                                                       <  SRR19973079.4266484/150‑1 (MQ=60)
                                                TAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCCCGATAT                                                                                                      >  SRR19973079.1844473/1‑150 (MQ=60)
                                                TAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATAT                                                                                                      >  SRR19973079.3888831/1‑150 (MQ=60)
                                                    TCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTG                                                                                                  <  SRR19973079.3848597/150‑1 (MQ=60)
                                                    TCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTG                                                                                                  <  SRR19973079.1009721/150‑1 (MQ=60)
                                                      TTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGAC                                                                                                <  SRR19973079.4281426/150‑1 (MQ=60)
                                                          CTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATT                                                                                            <  SRR19973079.143834/150‑1 (MQ=60)
                                                          CTGCTTAGACTAAATCTTTGCCATAAGAACAATATGTTCAAATCGAGGGGATTTATATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATT                                                                                            <  SRR19973079.141613/150‑1 (MQ=60)
                                                           TGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTA                                                                                           <  SRR19973079.2320301/150‑1 (MQ=60)
                                                           TGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTA                                                                                           <  SRR19973079.2320606/150‑1 (MQ=60)
                                                               TAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTT                                                                                       <  SRR19973079.767585/150‑1 (MQ=60)
                                                                AGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTA                                                                                      <  SRR19973079.1442400/150‑1 (MQ=60)
                                                                AGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTA                                                                                      <  SRR19973079.118097/150‑1 (MQ=60)
                                                                 GACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTAT                                                                                     <  SRR19973079.4220490/150‑1 (MQ=60)
                                                                 GACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTAT                                                                                     <  SRR19973079.4220579/150‑1 (MQ=60)
                                                                  ACTAAATCATTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATG                                                                                    <  SRR19973079.4283281/150‑1 (MQ=60)
                                                                   CTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGG                                                                                   <  SRR19973079.2508041/150‑1 (MQ=60)
                                                                    TAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGC                                                                                  >  SRR19973079.3883792/1‑150 (MQ=60)
                                                                    TAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGC                                                                                  >  SRR19973079.1052685/1‑150 (MQ=60)
                                                                    TAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGC                                                                                  >  SRR19973079.3883647/1‑150 (MQ=60)
                                                                     AAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCG                                                                                 <  SRR19973079.1559245/150‑1 (MQ=60)
                                                                     AAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGAGTATGTTATGGCG                                                                                 >  SRR19973079.380893/1‑150 (MQ=60)
                                                                      AATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGG                                                                                >  SRR19973079.2540944/1‑150 (MQ=60)
                                                                      AATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGG                                                                                >  SRR19973079.4015894/1‑150 (MQ=60)
                                                                      AATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGG                                                                                >  SRR19973079.4015925/1‑150 (MQ=60)
                                                                      AATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGG                                                                                >  SRR19973079.42977/1‑150 (MQ=60)
                                                                      AATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGG                                                                                >  SRR19973079.1816811/1‑150 (MQ=60)
                                                                         CTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCA                                                                             <  SRR19973079.1716689/150‑1 (MQ=60)
                                                                         CTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCA                                                                             <  SRR19973079.3917219/150‑1 (MQ=60)
                                                                          TTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCAT                                                                            <  SRR19973079.2245552/150‑1 (MQ=60)
                                                                          TTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCAT                                                                            >  SRR19973079.1577977/1‑150 (MQ=60)
                                                                          TTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCAT                                                                            <  SRR19973079.713270/150‑1 (MQ=60)
                                                                          TTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCAT                                                                            <  SRR19973079.713190/150‑1 (MQ=60)
                                                                          TTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCAT                                                                            <  SRR19973079.2245558/150‑1 (MQ=60)
                                                                          TTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCAT                                                                            <  SRR19973079.505393/150‑1 (MQ=60)
                                                                           TTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATG                                                                           <  SRR19973079.1994849/150‑1 (MQ=60)
                                                                               CATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTT                                                                       >  SRR19973079.339684/1‑150 (MQ=60)
                                                                                ATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTA                                                                      <  SRR19973079.2853277/150‑1 (MQ=60)
                                                                                ATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTA                                                                      <  SRR19973079.3365080/150‑1 (MQ=60)
                                                                                ATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTA                                                                      <  SRR19973079.4067148/150‑1 (MQ=60)
                                                                                 TAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGAGTATGTTATGGCGGGCATGTGTTAT                                                                     >  SRR19973079.249671/1‑150 (MQ=60)
                                                                                   AGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGAGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGC                                                                   <  SRR19973079.274901/150‑1 (MQ=60)
                                                                                    GAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCG                                                                  >  SRR19973079.3354787/1‑150 (MQ=60)
                                                                                    GAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCG                                                                  >  SRR19973079.1697380/1‑150 (MQ=60)
                                                                                     AACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGA                                                                 <  SRR19973079.182479/150‑1 (MQ=60)
                                                                                          AATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATAT                                                            <  SRR19973079.2224223/150‑1 (MQ=60)
                                                                                          AATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATAT                                                            <  SRR19973079.2224403/150‑1 (MQ=60)
                                                                                          AATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATAT                                                            >  SRR19973079.3524757/1‑150 (MQ=60)
                                                                                             GTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTA                                                         >  SRR19973079.973391/1‑150 (MQ=60)
                                                                                             GTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTA                                                         >  SRR19973079.3173626/1‑150 (MQ=60)
                                                                                                CAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGT                                                      >  SRR19973079.4297893/1‑150 (MQ=60)
                                                                                                CAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGT                                                      >  SRR19973079.4297911/1‑150 (MQ=60)
                                                                                                   ATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGC                                                   >  SRR19973079.1951130/1‑150 (MQ=60)
                                                                                                   ATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGC                                                   >  SRR19973079.3336267/1‑150 (MQ=60)
                                                                                                   ATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGC                                                   >  SRR19973079.2158887/1‑150 (MQ=60)
                                                                                                   ATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGC                                                   >  SRR19973079.330778/1‑150 (MQ=60)
                                                                                                    TCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCC                                                  <  SRR19973079.1970284/150‑1 (MQ=60)
                                                                                                    TCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCC                                                  <  SRR19973079.1971927/150‑1 (MQ=60)
                                                                                                      GAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCT                                                >  SRR19973079.1939650/1‑150 (MQ=60)
                                                                                                      GAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCT                                                >  SRR19973079.1939971/1‑150 (MQ=60)
                                                                                                       AGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTA                                               <  SRR19973079.543825/150‑1 (MQ=60)
                                                                                                        GGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTAT                                              <  SRR19973079.3234334/150‑1 (MQ=60)
                                                                                                          GGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCG                                            >  SRR19973079.546805/1‑150 (MQ=60)
                                                                                                          GGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCG                                            >  SRR19973079.2972286/1‑150 (MQ=60)
                                                                                                             TTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCAC                                         <  SRR19973079.3723336/150‑1 (MQ=60)
                                                                                                             TTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCAC                                         <  SRR19973079.3723244/150‑1 (MQ=60)
                                                                                                               TCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACAT                                       <  SRR19973079.3221713/150‑1 (MQ=60)
                                                                                                               TCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACAT                                       <  SRR19973079.3220328/150‑1 (MQ=60)
                                                                                                                 TATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGG                                     <  SRR19973079.2424161/150‑1 (MQ=60)
                                                                                                                    GGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTCAGGTCACCGGGCGGGCACGATATACTGACGATTAGGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTA                                  >  SRR19973079.3303260/1‑150 (MQ=60)
                                                                                                                     GAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTAT                                 <  SRR19973079.3358923/150‑1 (MQ=60)
                                                                                                                     GAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTAT                                 <  SRR19973079.533365/150‑1 (MQ=60)
                                                                                                                       AGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGC                               >  SRR19973079.1211455/1‑150 (MQ=60)
                                                                                                                        GCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCC                              <  SRR19973079.3428947/150‑1 (MQ=60)
                                                                                                                            GGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAA                          >  SRR19973079.2271603/1‑150 (MQ=60)
                                                                                                                            GGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAA                          <  SRR19973079.537134/150‑1 (MQ=60)
                                                                                                                            GGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAA                          <  SRR19973079.2724202/150‑1 (MQ=60)
                                                                                                                            GGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGAGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAA                          >  SRR19973079.2271727/1‑150 (MQ=60)
                                                                                                                             GGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAG                         >  SRR19973079.341882/1‑150 (MQ=60)
                                                                                                                              GAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGT                        <  SRR19973079.855245/150‑1 (MQ=60)
                                                                                                                              GAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGT                        >  SRR19973079.1246064/1‑150 (MQ=60)
                                                                                                                              GAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGT                        <  SRR19973079.3042886/150‑1 (MQ=60)
                                                                                                                                 GCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATT                     <  SRR19973079.3401926/150‑1 (MQ=60)
                                                                                                                                 GCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATT                     <  SRR19973079.3419968/150‑1 (MQ=60)
                                                                                                                                 GCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATT                     <  SRR19973079.928695/150‑1 (MQ=60)
                                                                                                                                 GCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATT                     <  SRR19973079.927342/150‑1 (MQ=60)
                                                                                                                                     CCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATG                 <  SRR19973079.4381251/150‑1 (MQ=60)
                                                                                                                                         TACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGA             >  SRR19973079.3909802/1‑150 (MQ=60)
                                                                                                                                         TACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGA             >  SRR19973079.3911226/1‑150 (MQ=60)
                                                                                                                                         TACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGA             >  SRR19973079.4171289/1‑150 (MQ=60)
                                                                                                                                         TACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGA             <  SRR19973079.3012206/150‑1 (MQ=60)
                                                                                                                                           CGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGAAC           >  SRR19973079.755664/1‑150 (MQ=60)
                                                                                                                                             GGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCATGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGAACAA         <  SRR19973079.18953/150‑1 (MQ=60)
                                                                                                                                              GTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGAACAAG        <  SRR19973079.3750412/150‑1 (MQ=60)
                                                                                                                                               TGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGAACAAGC       >  SRR19973079.35308/1‑150 (MQ=60)
                                                                                                                                               TGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGAACAAGC       >  SRR19973079.35301/1‑150 (MQ=60)
                                                                                                                                                    CATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGAACAAGCCAGAA  >  SRR19973079.1725108/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
CGTCATCACACTTCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGAACAAGCCAGAA  >  CP009273/2993551‑2993848

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑