Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F488 I0 R1 1275 300.1 10328444 91.0% 9398884 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,993,699 C→A 100% intergenic (‑449/‑5) ygeR ← / → xdhA LysM domain‑containing M23 family putative peptidase; septation lipoprotein/xanthine dehydrogenase, molybdenum binding subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,993,6990CA99.2% 877.7 / ‑2.7 265intergenic (‑449/‑5)ygeR/xdhALysM domain‑containing M23 family putative peptidase; septation lipoprotein/xanthine dehydrogenase, molybdenum binding subunit
Reads supporting (aligned to +/- strand):  ref base C (1/2);  new base A (132/130);  total (133/132)
Fisher's exact test for biased strand distribution p-value = 6.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 279 total aligned reads displayed.

GTCATCACACTTCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGAACAAGCCA  >  CP009273/2993552‑2993845
                                                                                                                                                   |                                                                                                                                                  
gTCATCACACTTCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGaataat                                                                                                                                                  <  2:1406743/150‑1 (MQ=255)
  catcaCACTTCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATaatgc                                                                                                                                                >  2:1634449/1‑150 (MQ=255)
   atcaCACTTCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGCTTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATaatgca                                                                                                                                               >  2:2191004/1‑150 (MQ=255)
    tcaCACTTCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATaatgcat                                                                                                                                              <  2:1687121/150‑1 (MQ=255)
     cacaCTTCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTTCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATTGAAGCGCGGGAAGCAACCGCTAAGGGTGAATaatgcatt                                                                                                                                             >  1:2364502/1‑149 (MQ=255)
      acacTTCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAatgcatgc                                                                                                                                            <  1:3671001/150‑1 (MQ=255)
      acacTTCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAatgcatgc                                                                                                                                            >  2:1547159/1‑150 (MQ=255)
      acacTTCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATAGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAatgcatgc                                                                                                                                            <  2:3716579/150‑1 (MQ=255)
       cacTTCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATgcg                                                                                                                                           >  2:2554519/1‑150 (MQ=255)
          ttCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGt                                                                                                                                        >  2:2233143/1‑150 (MQ=255)
                ccTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGc                                                                                                                                  >  1:710353/1‑150 (MQ=255)
                  tCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCa                                                                                                                                >  2:1902340/1‑150 (MQ=255)
                  tCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCa                                                                                                                                <  1:2594764/150‑1 (MQ=255)
                  tCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCa                                                                                                                                <  1:5151801/150‑1 (MQ=255)
                  tCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCa                                                                                                                                >  2:4280358/1‑150 (MQ=255)
                  tCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCa                                                                                                                                >  2:3307326/1‑150 (MQ=255)
                  tCCCCTAACGCGTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCa                                                                                                                                <  1:2411344/150‑1 (MQ=255)
                    cccTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCAtt                                                                                                                              >  1:503396/1‑150 (MQ=255)
                              tATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCCCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCa                                                                                                                    >  2:3041954/1‑150 (MQ=255)
                                  gtcgtTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGCAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACcgg                                                                                                                >  2:4798010/1‑150 (MQ=255)
                                   tcgtTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACcggg                                                                                                               <  2:1639919/150‑1 (MQ=255)
                                     gtTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACcgggcg                                                                                                             <  1:2032269/150‑1 (MQ=255)
                                     gtTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACcgggcg                                                                                                             >  1:4113277/1‑150 (MQ=255)
                                      tttCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACcgggcgg                                                                                                            <  1:5054362/150‑1 (MQ=255)
                                      tttATGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGTAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACcgggcgg                                                                                                            >  1:2933555/1‑150 (MQ=255)
                                       ttCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACcgggcggg                                                                                                           >  1:4424410/1‑150 (MQ=255)
                                         cTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCa                                                                                                         <  1:4051110/150‑1 (MQ=255)
                                         cTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCa                                                                                                         >  2:1040745/1‑150 (MQ=255)
                                          tGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCAc                                                                                                        >  2:537398/1‑150 (MQ=255)
                                             cGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGat                                                                                                     <  1:1296510/150‑1 (MQ=255)
                                               tAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGatat                                                                                                   <  2:4490480/150‑1 (MQ=255)
                                                 aaTCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATAc                                                                                                 >  1:280462/1‑150 (MQ=255)
                                                 aaTCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATAc                                                                                                 >  1:2873884/1‑150 (MQ=255)
                                                 aaTCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATAc                                                                                                 >  1:2982877/1‑150 (MQ=255)
                                                  aTCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACt                                                                                                <  1:4299677/150‑1 (MQ=255)
                                                    cTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGa                                                                                              >  2:2066189/1‑150 (MQ=255)
                                                    cTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGa                                                                                              >  1:4754407/1‑150 (MQ=255)
                                                     ttGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGAc                                                                                             >  2:12473/1‑150 (MQ=255)
                                                      tGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACg                                                                                            <  1:883459/150‑1 (MQ=255)
                                                             ttAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGAttatgt                                                                                     >  1:522311/1‑150 (MQ=255)
                                                                   tAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGAGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCCTTGCTAAGGTCACCGGGCGGGCACTATATACTGACGATTATGTTATGGc                                                                               <  2:2453225/150‑1 (MQ=255)
                                                                    aaaTCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCg                                                                              <  2:3840331/150‑1 (MQ=255)
                                                                    aaaTCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCg                                                                              <  1:907497/150‑1 (MQ=255)
                                                                      aTCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCggg                                                                            >  1:542854/1‑150 (MQ=255)
                                                                      aTCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCggg                                                                            <  1:2510299/150‑1 (MQ=255)
                                                                       tCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGc                                                                           <  1:4059147/150‑1 (MQ=255)
                                                                        cTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCa                                                                          >  2:4245256/1‑150 (MQ=255)
                                                                           tGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCAtgt                                                                       >  2:2362570/1‑150 (MQ=255)
                                                                            gCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCAtgtg                                                                      <  1:134301/150‑1 (MQ=255)
                                                                             ccATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATgtgt                                                                     >  2:2632948/1‑150 (MQ=255)
                                                                                    aaCCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTGATGGCGGGCATGTGTTATGCgt                                                              >  2:4404512/1‑149 (MQ=255)
                                                                                      ccAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGaaa                                                            <  2:4010273/150‑1 (MQ=255)
                                                                                      ccAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGaaa                                                            <  1:1705103/150‑1 (MQ=255)
                                                                                      ccAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGaaa                                                            <  1:2066189/150‑1 (MQ=255)
                                                                                          aTGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATg                                                        <  1:4653729/150‑1 (MQ=255)
                                                                                          aTGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATg                                                        <  1:1191263/150‑1 (MQ=255)
                                                                                           tGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGt                                                       >  1:4530619/1‑150 (MQ=255)
                                                                                           tGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGt                                                       <  1:3546471/150‑1 (MQ=255)
                                                                                              tCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACg                                                    >  1:2181213/1‑150 (MQ=255)
                                                                                              tCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACg                                                    <  2:1123456/150‑1 (MQ=255)
                                                                                              tCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACg                                                    <  2:3365797/150‑1 (MQ=255)
                                                                                              tCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACg                                                    >  1:4795255/1‑150 (MQ=255)
                                                                                              tCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACg                                                    >  2:3554886/1‑150 (MQ=255)
                                                                                                aaaTCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTa                                                  >  2:1819912/1‑150 (MQ=255)
                                                                                                  aTCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGc                                                >  2:2827101/1‑150 (MQ=255)
                                                                                                   tCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGcc                                               <  2:887849/150‑1 (MQ=255)
                                                                                                    cGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGccc                                              >  1:4148349/1‑150 (MQ=255)
                                                                                                    cGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGccc                                              >  1:400530/1‑150 (MQ=255)
                                                                                                     gAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCt                                             >  2:4467049/1‑150 (MQ=255)
                                                                                                     gAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCt                                             <  1:3686340/150‑1 (MQ=255)
                                                                                                     gAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCt                                             <  1:3886551/150‑1 (MQ=255)
                                                                                                         ggATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATca                                         <  2:139827/150‑2 (MQ=255)
                                                                                                           aTTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGca                                       <  2:280462/150‑1 (MQ=255)
                                                                                                           aTTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGca                                       <  2:2873884/150‑1 (MQ=255)
                                                                                                             ttCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATCTGTACGTAGCCCTATCGcaca                                     >  2:4020976/1‑150 (MQ=255)
                                                                                                             ttCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGcaca                                     >  1:1397800/1‑150 (MQ=255)
                                                                                                               cTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATg                                   >  2:4169806/1‑150 (MQ=255)
                                                                                                                 aTGGAAGCGCTGGAAGAAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGt                                 >  2:3187406/1‑150 (MQ=255)
                                                                                                                  tGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGtt                                <  1:1668564/150‑1 (MQ=255)
                                                                                                                  tGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGtt                                <  2:1317777/150‑1 (MQ=255)
                                                                                                                   ggAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTa                               <  2:1977748/150‑1 (MQ=255)
                                                                                                                   ggAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTa                               <  2:2507576/150‑1 (MQ=255)
                                                                                                                       gcgcGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGcc                           <  2:4692068/150‑1 (MQ=255)
                                                                                                                        cgcgGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCg                          <  1:1902340/150‑1 (MQ=255)
                                                                                                                         gcgGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGt                         <  1:3078970/150‑1 (MQ=255)
                                                                                                                           gggAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTaa                       >  1:1021349/1‑150 (MQ=255)
                                                                                                                            ggAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAg                      >  1:4848724/1‑150 (MQ=255)
                                                                                                                            ggAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAg                      >  2:357130/1‑150 (MQ=255)
                                                                                                                             gAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGt                     <  2:1291966/150‑1 (MQ=255)
                                                                                                                              aaGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTa                    >  1:31680/1‑150 (MQ=255)
                                                                                                                              aaGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTa                    >  1:1024648/1‑150 (MQ=255)
                                                                                                                                gCAACGGCTACGTGTGAATAATGCATCCGCGTCGATGCCATTGCTAAGGTCAGCGGGCGGGCACTATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTAtt                  <  2:5081006/150‑1 (MQ=255)
                                                                                                                                gCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTAtt                  <  1:3307326/150‑1 (MQ=255)
                                                                                                                                gCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTAtt                  >  1:770474/1‑150 (MQ=255)
                                                                                                                                  aaCCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTaa                >  2:3459823/1‑150 (MQ=255)
                                                                                                                                   aCCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAat               <  2:4154144/150‑1 (MQ=255)
                                                                                                                                          cGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGAAc        >  2:2125022/1‑150 (MQ=255)
                                                                                                                                            ggTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGAACaa      >  2:1759570/1‑150 (MQ=255)
                                                                                                                                              tGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGAACAAGc    <  1:976658/150‑1 (MQ=255)
                                                                                                                                                aataatGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGAACAAGCCa  <  2:629254/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GTCATCACACTTCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGAACAAGCCA  >  CP009273/2993552‑2993845

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACGTCATCACACTTCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGAACAAGCCAG  >  CP009273/2993550‑2993846
                                                                                                                                                     |                                                                                                                                                   
ACGTCATCACACTTCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAAACAAATGTTCAAATCGAGGGGATTTATATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATA                                                                                                                                                     >  SRR19973063.2354764/1‑150 (MQ=60)
 CGTCATCACACTTCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAA                                                                                                                                                    >  SRR19973063.1126868/1‑150 (MQ=60)
     ATCACACTTCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCA                                                                                                                                                <  SRR19973063.5063581/150‑1 (MQ=60)
     ATCACACTTCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCA                                                                                                                                                <  SRR19973063.5063730/150‑1 (MQ=60)
     ATCACACTTCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATATTGCCTGCTTAGACTAAATCATTGCCATAATAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTAAGGGTGAATAATTCA                                                                                                                                                >  SRR19973063.2852591/1‑150 (MQ=60)
       CACACTTCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTTCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATTGAAGCGCGGGAAGCAACCGCTAAGGGTGAATAATGCATT                                                                                                                                              >  SRR19973063.2372080/1‑150 (MQ=60)
        ACACTTCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGC                                                                                                                                             <  SRR19973063.3682759/150‑1 (MQ=60)
           CTTCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCG                                                                                                                                          >  SRR19973063.707068/1‑150 (MQ=60)
              CCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCG                                                                                                                                       <  SRR19973063.1324962/150‑1 (MQ=60)
               CTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATGTTTGCCATAAGAACCAAATGTTAAAATCGAGGGGATTTCTATGGCAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATTCGCGTCGA                                                                                                                                      >  SRR19973063.4023137/1‑150 (MQ=60)
                  CCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGC                                                                                                                                   >  SRR19973063.712441/1‑150 (MQ=60)
                    TCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAATAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCAAGCGCGTCGATGCCA                                                                                                                                 >  SRR19973063.1475451/1‑150 (MQ=60)
                    TCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCA                                                                                                                                 <  SRR19973063.2603009/150‑1 (MQ=60)
                    TCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCA                                                                                                                                 <  SRR19973063.1941757/150‑1 (MQ=60)
                    TCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCA                                                                                                                                 <  SRR19973063.5168379/150‑1 (MQ=60)
                    TCCCCTAACGCGTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCA                                                                                                                                 <  SRR19973063.2419058/150‑1 (MQ=60)
                     CCCCTAACGCTTATCGTCGTTTCTGGCGTAAATATTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCAT                                                                                                                                >  SRR19973063.895016/1‑150 (MQ=60)
                      CCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATT                                                                                                                               >  SRR19973063.504852/1‑150 (MQ=60)
                       CCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTG                                                                                                                              <  SRR19973063.2953365/150‑1 (MQ=60)
                       CCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTG                                                                                                                              >  SRR19973063.3170108/1‑150 (MQ=60)
                        CTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGC                                                                                                                             >  SRR19973063.5103327/1‑150 (MQ=60)
                          AACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTA                                                                                                                           <  SRR19973063.1935645/150‑1 (MQ=60)
                            CGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAG                                                                                                                         <  SRR19973063.3967132/150‑1 (MQ=60)
                                TATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCA                                                                                                                     <  SRR19973063.2744420/150‑1 (MQ=60)
                                  TCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACC                                                                                                                   >  SRR19973063.4806203/1‑150 (MQ=60)
                                   CGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCG                                                                                                                  <  SRR19973063.2127419/150‑1 (MQ=60)
                                       GTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCG                                                                                                              >  SRR19973063.4126452/1‑150 (MQ=60)
                                       GTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCG                                                                                                              <  SRR19973063.4617343/150‑1 (MQ=60)
                                       GTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCG                                                                                                              <  SRR19973063.2038623/150‑1 (MQ=60)
                                        TTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGG                                                                                                             <  SRR19973063.5070635/150‑1 (MQ=60)
                                        TTTATGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGTAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGG                                                                                                             >  SRR19973063.2942907/1‑150 (MQ=60)
                                         TTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGG                                                                                                            >  SRR19973063.4438614/1‑150 (MQ=60)
                                           CTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCA                                                                                                          <  SRR19973063.4064073/150‑1 (MQ=60)
                                           CTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCA                                                                                                          <  SRR19973063.4064101/150‑1 (MQ=60)
                                           CTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCA                                                                                                          <  SRR19973063.4064096/150‑1 (MQ=60)
                                           CTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCA                                                                                                          <  SRR19973063.3697097/150‑1 (MQ=60)
                                           CTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCA                                                                                                          <  SRR19973063.1680966/150‑1 (MQ=60)
                                           CTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCA                                                                                                          <  SRR19973063.1680997/150‑1 (MQ=60)
                                           CTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCA                                                                                                          <  SRR19973063.5177811/150‑1 (MQ=60)
                                            TGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCAC                                                                                                         <  SRR19973063.71839/150‑1 (MQ=60)
                                            TGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGAGGGAAGCAACCTCTACGGGTGAATAATGCATGCTCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCAC                                                                                                         <  SRR19973063.3258931/150‑1 (MQ=60)
                                               CGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGAT                                                                                                      <  SRR19973063.1300367/150‑1 (MQ=60)
                                                 TAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATAT                                                                                                    <  SRR19973063.2121582/150‑1 (MQ=60)
                                                  AAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATA                                                                                                   >  SRR19973063.3556365/1‑150 (MQ=60)
                                                  AAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATA                                                                                                   >  SRR19973063.4173786/1‑150 (MQ=60)
                                                   AATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATAC                                                                                                  >  SRR19973063.5174105/1‑150 (MQ=60)
                                                   AATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATAC                                                                                                  >  SRR19973063.281294/1‑150 (MQ=60)
                                                   AATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATAC                                                                                                  >  SRR19973063.2883078/1‑150 (MQ=60)
                                                   AATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATAC                                                                                                  >  SRR19973063.2992404/1‑150 (MQ=60)
                                                    ATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACT                                                                                                 <  SRR19973063.4313341/150‑1 (MQ=60)
                                                      CTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGA                                                                                               >  SRR19973063.4769565/1‑150 (MQ=60)
                                                      CTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATAGAGGGGATTTCTATGGAAGCGCGGGAAGAAACCGCTAAGTGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGA                                                                                               >  SRR19973063.4769320/1‑150 (MQ=60)
                                                       TTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGAC                                                                                              <  SRR19973063.2889697/150‑1 (MQ=60)
                                                        TGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACG                                                                                             >  SRR19973063.3903730/1‑150 (MQ=60)
                                                        TGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACG                                                                                             <  SRR19973063.886013/150‑1 (MQ=60)
                                                           CTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATT                                                                                          >  SRR19973063.247835/1‑150 (MQ=60)
                                                           CTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATT                                                                                          <  SRR19973063.2928598/150‑1 (MQ=60)
                                                           CTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGAGT                                                                                          >  SRR19973063.4129096/1‑150 (MQ=60)
                                                             GCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTAT                                                                                        >  SRR19973063.890416/1‑150 (MQ=60)
                                                              CTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATG                                                                                       >  SRR19973063.4538355/1‑150 (MQ=60)
                                                               TTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGT                                                                                      >  SRR19973063.85347/1‑150 (MQ=60)
                                                               TTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGT                                                                                      >  SRR19973063.523839/1‑150 (MQ=60)
                                                                 AGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTA                                                                                    <  SRR19973063.4795661/150‑1 (MQ=60)
                                                                 AGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTA                                                                                    <  SRR19973063.4383965/150‑1 (MQ=60)
                                                                  GACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTAT                                                                                   >  SRR19973063.1208436/1‑150 (MQ=60)
                                                                     TAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGC                                                                                >  SRR19973063.503627/1‑150 (MQ=60)
                                                                      AAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCG                                                                               >  SRR19973063.4094612/1‑150 (MQ=60)
                                                                      AAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCG                                                                               <  SRR19973063.910154/150‑1 (MQ=60)
                                                                        ATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGG                                                                             >  SRR19973063.5119363/1‑150 (MQ=60)
                                                                        ATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGG                                                                             <  SRR19973063.4818005/150‑1 (MQ=60)
                                                                        ATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGG                                                                             >  SRR19973063.544461/1‑150 (MQ=60)
                                                                        ATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGG                                                                             <  SRR19973063.2518302/150‑1 (MQ=60)
                                                                         TCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGC                                                                            <  SRR19973063.4072155/150‑1 (MQ=60)
                                                                            tgatgagcgggttttgtgctttaaatggggcaatggtaatgttgaatctcatcccgggactcatgtcGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATG                                                                         >  SRR19973063.3059178/68‑150 (MQ=60)
                                                                            TTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATG                                                                         >  SRR19973063.2281076/1‑150 (MQ=60)
                                                                            TTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATG                                                                         <  SRR19973063.4823909/150‑1 (MQ=60)
                                                                              GCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTG                                                                       <  SRR19973063.134724/150‑1 (MQ=60)
                                                                              GCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTG                                                                       <  SRR19973063.135036/150‑1 (MQ=60)
                                                                                 ATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTA                                                                    >  SRR19973063.1484769/1‑150 (MQ=60)
                                                                                    AGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGC                                                                 <  SRR19973063.3641227/150‑1 (MQ=60)
                                                                                    AGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGC                                                                 <  SRR19973063.1894548/150‑1 (MQ=60)
                                                                                        CCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAA                                                             <  SRR19973063.2072648/150‑1 (MQ=60)
                                                                                        CCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAA                                                             <  SRR19973063.2072549/150‑1 (MQ=60)
                                                                                        CCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAA                                                             <  SRR19973063.1710300/150‑1 (MQ=60)
                                                                                           AATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATAT                                                          <  SRR19973063.1039746/150‑1 (MQ=60)
                                                                                           AATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATAT                                                          >  SRR19973063.3065501/1‑150 (MQ=60)
                                                                                           AATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATAT                                                          <  SRR19973063.3115834/150‑1 (MQ=60)
                                                                                            ATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATG                                                         <  SRR19973063.1194843/150‑1 (MQ=60)
                                                                                            ATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATG                                                         <  SRR19973063.4668586/150‑1 (MQ=60)
                                                                                             TGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGACTAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGT                                                        >  SRR19973063.422516/1‑150 (MQ=60)
                                                                                             TGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGT                                                        <  SRR19973063.3557835/150‑1 (MQ=60)
                                                                                             TGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGT                                                        >  SRR19973063.4545149/1‑150 (MQ=60)
                                                                                               TTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTAC                                                      <  SRR19973063.4085320/150‑1 (MQ=60)
                                                                                                TCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACG                                                     >  SRR19973063.4810576/1‑150 (MQ=60)
                                                                                                TCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACG                                                     >  SRR19973063.2188040/1‑150 (MQ=60)
                                                                                                   AATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAG                                                  >  SRR19973063.2404944/1‑150 (MQ=60)
                                                                                                      CGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCC                                               >  SRR19973063.4161613/1‑150 (MQ=60)
                                                                                                      CGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCC                                               >  SRR19973063.4161619/1‑150 (MQ=60)
                                                                                                      CGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCC                                               >  SRR19973063.3290668/1‑150 (MQ=60)
                                                                                                      CGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCC                                               >  SRR19973063.401661/1‑150 (MQ=60)
                                                                                                       GAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCT                                              <  SRR19973063.3698144/150‑1 (MQ=60)
                                                                                                       GAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCT                                              <  SRR19973063.3898895/150‑1 (MQ=60)
                                                                                                       GAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCT                                              >  SRR19973063.243707/1‑150 (MQ=60)
                                                                                                       GAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCT                                              <  SRR19973063.3698042/150‑1 (MQ=60)
                                                                                                        AGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTA                                             <  SRR19973063.3827012/150‑1 (MQ=60)
                                                                                                          GGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATC                                           <  SRR19973063.4555845/150‑1 (MQ=60)
                                                                                                               TTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACA                                      <  SRR19973063.2562657/150‑1 (MQ=60)
                                                                                                               TTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACA                                      >  SRR19973063.1402028/1‑150 (MQ=60)
                                                                                                               TTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACA                                      <  SRR19973063.2951418/150‑1 (MQ=60)
                                                                                                                 CTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATG                                    <  SRR19973063.538985/150‑1 (MQ=60)
                                                                                                                   ATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGT                                  >  SRR19973063.565790/1‑150 (MQ=60)
                                                                                                                    TGGAAGCGCGGGAAGCAACCGCTACGTGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTT                                 <  SRR19973063.286131/150‑1 (MQ=60)
                                                                                                                    TGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGTCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTT                                 <  SRR19973063.1100206/150‑1 (MQ=60)
                                                                                                                    TGGAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTT                                 <  SRR19973063.1673653/150‑1 (MQ=60)
                                                                                                                      GAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTAT                               >  SRR19973063.470136/1‑150 (MQ=60)
                                                                                                                      GAAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTAT                               <  SRR19973063.2244670/150‑1 (MQ=60)
                                                                                                                       AAGCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATTCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATG                              >  SRR19973063.3861695/1‑150 (MQ=60)
                                                                                                                         GCGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCC                            <  SRR19973063.2411324/150‑1 (MQ=60)
                                                                                                                          CGCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCG                           <  SRR19973063.1908291/150‑1 (MQ=60)
                                                                                                                           GCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGT                          <  SRR19973063.3088869/150‑1 (MQ=60)
                                                                                                                           GCGGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCAGT                          <  SRR19973063.3409625/150‑1 (MQ=60)
                                                                                                                             GGGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAA                        >  SRR19973063.1024426/1‑150 (MQ=60)
                                                                                                                              GGAAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAG                       >  SRR19973063.4864329/1‑150 (MQ=60)
                                                                                                                                AAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTACGTA                     >  SRR19973063.1027456/1‑150 (MQ=60)
                                                                                                                                AAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTA                     >  SRR19973063.31807/1‑150 (MQ=60)
                                                                                                                                AAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTA                     >  SRR19973063.2642227/1‑150 (MQ=60)
                                                                                                                                AAGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTA                     >  SRR19973063.1027739/1‑150 (MQ=60)
                                                                                                                                 AGCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTAT                    >  SRR19973063.1324614/1‑150 (MQ=60)
                                                                                                                                  GCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATT                   <  SRR19973063.3317903/150‑1 (MQ=60)
                                                                                                                                  GCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATT                   >  SRR19973063.772678/1‑150 (MQ=60)
                                                                                                                                  GCAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATT                   >  SRR19973063.772719/1‑150 (MQ=60)
                                                                                                                                   CAACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTA                  <  SRR19973063.4393009/150‑1 (MQ=60)
                                                                                                                                     ACCGCTACGGGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAAT                <  SRR19973063.1225653/150‑1 (MQ=60)
                                                                                                                                             GTGTGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGAACA        >  SRR19973063.1816522/1‑150 (MQ=60)
                                                                                                                                                TGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGAACAAGC     <  SRR19973063.979508/150‑1 (MQ=60)
                                                                                                                                                TGAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGAACAAGC     <  SRR19973063.979602/150‑1 (MQ=60)
                                                                                                                                                 GAATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGAACAAGCC    >  SRR19973063.845362/1‑150 (MQ=60)
                                                                                                                                                   ATAATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGAACAAGCCAG  <  SRR19973063.4958579/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                   
ACGTCATCACACTTCCTACCTCCCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGAACAAGCCAG  >  CP009273/2993550‑2993846

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑