Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F247 I0 R1	1496	299.8	9582018	97.8%	9371213	149.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,993,211	C→A	100%	V14L (GTG→TTG)	ygeR ←	LysM domain‑containing M23 family putative peptidase; septation lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,993,211	0	C	A	99.2%	881.9 / ‑1.7	261	V14L (GTG→TTG)	ygeR	LysM domain‑containing M23 family putative peptidase; septation lipoprotein
Reads supporting (aligned to +/- strand): ref base C (1/1); new base A (104/155); total (105/156)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 261 total aligned reads displayed.

TTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAGGTTAATT  >  CP009273/2993065‑2993356

ttttACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATATGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGa                                                                                                                                                 <  1:189769/149‑1 (MQ=255)

ttttACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGa                                                                                                                                                 <  1:1718696/149‑1 (MQ=255)

    aCGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATAcc                                                                                                                                             <  1:2695962/149‑1 (MQ=255)

    aCGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATAcc                                                                                                                                             <  1:483428/149‑1 (MQ=255)

      gCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCa                                                                                                                                           <  1:657327/149‑1 (MQ=255)

        tGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCaga                                                                                                                                         >  1:859571/1‑149 (MQ=255)

           ttCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGAt                                                                                                                                      >  2:116719/1‑149 (MQ=255)

           ttCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGAt                                                                                                                                      <  1:4652195/149‑1 (MQ=255)

           ttCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGAt                                                                                                                                      >  2:4023421/1‑149 (MQ=255)

              ccGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGAtttt                                                                                                                                   <  1:4448770/149‑1 (MQ=255)

                gtggtgCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGAtttttt                                                                                                                                 >  2:4723058/1‑149 (MQ=255)

                 tggtgCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTa                                                                                                                                <  1:3121574/149‑1 (MQ=255)

                  ggtgCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTAt                                                                                                                               <  2:2140880/149‑1 (MQ=255)

                   gtgCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTAtt                                                                                                                              <  1:3183188/149‑1 (MQ=255)

                          aaaTACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGt                                                                                                                       >  2:3046813/1‑149 (MQ=255)

                           aaTACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTc                                                                                                                      >  1:3907761/1‑149 (MQ=255)

                           aaTACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTc                                                                                                                      <  1:2003509/149‑1 (MQ=255)

                            aTACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTcc                                                                                                                     <  2:4376204/149‑1 (MQ=255)

                            aTACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTcc                                                                                                                     <  1:971824/149‑1 (MQ=255)

                              aCGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCg                                                                                                                   >  2:4440680/1‑149 (MQ=255)

                                 atatAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCAc                                                                                                                >  2:1813519/1‑149 (MQ=255)

                                 atatAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCAc                                                                                                                >  2:1461009/1‑149 (MQ=255)

                                      gCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCaaa                                                                                                           >  2:2841519/1‑149 (MQ=255)

                                       cGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCaaaa                                                                                                          <  2:695048/149‑1 (MQ=255)

                                        gTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAAt                                                                                                         >  2:2734686/1‑149 (MQ=255)

                                        gTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAAt                                                                                                         >  1:1446163/1‑149 (MQ=255)

                                             cccccGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTc                                                                                                    <  1:3836698/149‑1 (MQ=255)

                                               cccGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTCTATTCAGGCGTCCCGCACTCAAAATCTTTCCt                                                                                                  <  1:3370889/149‑1 (MQ=255)

                                                  gTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGc                                                                                               >  1:4051313/1‑149 (MQ=255)

                                                  gTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGc                                                                                               <  2:4286554/149‑1 (MQ=255)

                                                   tttCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCt                                                                                              <  2:858882/149‑1 (MQ=255)

                                                    ttCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCtt                                                                                             <  2:3184776/149‑1 (MQ=255)

                                                      cACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTaa                                                                                           >  1:2568858/1‑149 (MQ=255)

                                                            gtAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACa                                                                                     >  1:2974455/1‑149 (MQ=255)

                                                            gtAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACa                                                                                     >  1:582490/1‑149 (MQ=255)

                                                             tAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACaa                                                                                    >  1:35450/1‑149 (MQ=255)

                                                              aaaCGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAg                                                                                   >  2:2835155/1‑149 (MQ=255)

                                                              aaaCGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAg                                                                                   <  2:4190951/149‑1 (MQ=255)

                                                              aaaCGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAg                                                                                   >  2:525413/1‑149 (MQ=255)

                                                              aaaCGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAg                                                                                   <  1:4298411/149‑1 (MQ=255)

                                                               aaCGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGa                                                                                  <  1:4756080/149‑1 (MQ=255)

                                                               aaCGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGa                                                                                  <  1:749172/149‑1 (MQ=255)

                                                                ccgtaGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCAAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGAc                                                                                 <  2:447655/145‑1 (MQ=255)

                                                                aCGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGAc                                                                                 >  1:1199935/1‑149 (MQ=255)

                                                                    aGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCt                                                                             <  1:1694333/149‑1 (MQ=255)

                                                                    aGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCt                                                                             <  2:4691156/149‑1 (MQ=255)

                                                                      ccGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTa                                                                           <  1:2904693/149‑1 (MQ=255)

                                                                         gAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCa                                                                        <  1:4173219/149‑1 (MQ=255)

                                                                         gAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCa                                                                        <  1:4458102/149‑1 (MQ=255)

                                                                         gAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCa                                                                        >  1:4655798/1‑149 (MQ=255)

                                                                          aaTACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCAt                                                                       >  1:1057940/1‑149 (MQ=255)

                                                                           aTACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATa                                                                      <  2:3860424/149‑1 (MQ=255)

                                                                            tACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATag                                                                     <  1:123305/149‑1 (MQ=255)

                                                                              cGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATagca                                                                   >  1:2622416/1‑149 (MQ=255)

                                                                               gTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATagcag                                                                  <  1:3808578/149‑1 (MQ=255)

                                                                               gTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATagcag                                                                  <  1:3339143/149‑1 (MQ=255)

                                                                                ttCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATaacagc                                                                 <  2:3281851/149‑1 (MQ=255)

                                                                                      tATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCct                                                           <  2:4069591/149‑1 (MQ=255)

                                                                                      tATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCct                                                           >  2:2476748/1‑149 (MQ=255)

                                                                                       aTCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCctc                                                          <  2:3410183/149‑1 (MQ=255)

                                                                                       aTCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCctc                                                          <  2:2038770/149‑1 (MQ=255)

                                                                                       aTCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCctc                                                          >  1:3793779/1‑149 (MQ=255)

                                                                                         cGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCtt                                                        <  2:1586250/149‑1 (MQ=255)

                                                                                         cGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCtt                                                        >  1:3223103/1‑149 (MQ=255)

                                                                                             tgatTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATa                                                    <  1:1061586/149‑1 (MQ=255)

                                                                                              gatTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATAc                                                   <  2:3848848/149‑1 (MQ=255)

                                                                                                      aCCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAAtctc                                           <  2:1490482/149‑1 (MQ=255)

                                                                                                         cGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTg                                        >  2:1336450/1‑149 (MQ=255)

                                                                                                          gAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGc                                       <  2:3811395/149‑1 (MQ=255)

                                                                                                            aCAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCtt                                     <  2:1535197/149‑1 (MQ=255)

                                                                                                                  cGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTCTCATAGCAGCCAGCCTCGTGATACCCAATCTCTTGCTTCTGGaa                               >  2:773232/1‑149 (MQ=255)

                                                                                                                  cGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGaa                               <  1:3278474/149‑1 (MQ=255)

                                                                                                                      aaaaGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAAc                           >  1:2237599/1‑149 (MQ=255)

                                                                                                                          gCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTgg                       >  1:416392/1‑149 (MQ=255)

                                                                                                                          gCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTgg                       <  1:2076023/149‑1 (MQ=255)

                                                                                                                             gTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTaa                    >  2:3041491/1‑149 (MQ=255)

                                                                                                                             gTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTaa                    >  2:2632032/1‑149 (MQ=255)

                                                                                                                              tCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAAt                   <  1:650803/149‑1 (MQ=255)

                                                                                                                               ccAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATa                  <  1:2835982/149‑1 (MQ=255)

                                                                                                                               caaaCCTATAACAACAACAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATa                  <  1:3689134/147‑1 (MQ=255)

                                                                                                                               acAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATa                  <  1:3910301/148‑1 (MQ=255)

                                                                                                                                 aaCCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATaga                <  2:3764837/149‑1 (MQ=255)

                                                                                                                                   ccGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATGgaga              >  2:3927250/1‑149 (MQ=255)

                                                                                                                                   ccGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAgaga              <  1:1582603/149‑1 (MQ=255)

                                                                                                                                    cGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTTGTAATAGAGaa             <  1:180206/149‑1 (MQ=255)

                                                                                                                                     gATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGaaa            <  2:3973649/149‑1 (MQ=255)

                                                                                                                                      aTAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAag           <  2:1437270/149‑1 (MQ=255)

                                                                                                                                      aTAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAag           <  2:2902151/149‑1 (MQ=255)

                                                                                                                                      aTAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAag           >  2:2376610/1‑149 (MQ=255)

                                                                                                                                       tAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGACTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATATAGAAaga          >  1:2332713/1‑149 (MQ=255)

                                                                                                                                        aacaacATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAagag         >  2:1020253/1‑149 (MQ=255)

                                                                                                                                         acaacaTCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAgg        >  1:3750152/1‑149 (MQ=255)

                                                                                                                                          caacaTCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAGGt       >  2:4239282/1‑149 (MQ=255)

                                                                                                                                          caacaTCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAGGt       <  1:2094403/149‑1 (MQ=255)

                                                                                                                                            acaTCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAGGTTa     <  2:2278254/149‑1 (MQ=255)

                                                                                                                                            acaTCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAGGTTa     >  2:1955291/1‑149 (MQ=255)

                                                                                                                                            acaTCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAGGTTa     >  2:1691546/1‑149 (MQ=255)

                                                                                                                                            acaTCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAGGTTa     >  2:36649/1‑149 (MQ=255)

                                                                                                                                             caTCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATCGAGAAAGAGGTTaa    >  1:1536288/1‑149 (MQ=255)

                                                                                                                                               tCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAGGTTAAtt  <  1:3637871/149‑1 (MQ=255)

TTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAGGTTAATT  >  CP009273/2993065‑2993356

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAGGTTAATTTCAT  >  CP009273/2993065‑2993360

TTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATATGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGAT                                                                                                                                                    <  SRR19973071.190558/150‑1 (MQ=60)

TTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGAT                                                                                                                                                    <  SRR19973071.1725089/150‑1 (MQ=60)

   TACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACC                                                                                                                                                 >  SRR19973071.4121768/1‑150 (MQ=60)

    ACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCC                                                                                                                                                <  SRR19973071.354913/150‑1 (MQ=60)

    ACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCC                                                                                                                                                <  SRR19973071.2755881/150‑1 (MQ=60)

    ACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCC                                                                                                                                                <  SRR19973071.2705588/150‑1 (MQ=60)

    ACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCC                                                                                                                                                <  SRR19973071.1930839/150‑1 (MQ=60)

    ACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCC                                                                                                                                                <  SRR19973071.485213/150‑1 (MQ=60)

    ACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCC                                                                                                                                                <  SRR19973071.2167647/150‑1 (MQ=60)

      GCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAG                                                                                                                                              <  SRR19973071.659258/150‑1 (MQ=60)

      GCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAG                                                                                                                                              <  SRR19973071.659715/150‑1 (MQ=60)

       CTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGA                                                                                                                                             >  SRR19973071.862643/1‑150 (MQ=60)

           TTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATT                                                                                                                                         <  SRR19973071.4668605/150‑1 (MQ=60)

           TTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATT                                                                                                                                         <  SRR19973071.4668763/150‑1 (MQ=60)

             CCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTT                                                                                                                                       <  SRR19973071.3729855/150‑1 (MQ=60)

              CCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTT                                                                                                                                      <  SRR19973071.4464926/150‑1 (MQ=60)

              CCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTT                                                                                                                                      <  SRR19973071.4464504/150‑1 (MQ=60)

              CCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTT                                                                                                                                      <  SRR19973071.4414756/150‑1 (MQ=60)

                GTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTA                                                                                                                                    <  SRR19973071.1752414/150‑1 (MQ=60)

                GTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTA                                                                                                                                    <  SRR19973071.4167808/150‑1 (MQ=60)

                 TGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTAT                                                                                                                                   <  SRR19973071.3132553/150‑1 (MQ=60)

                 TGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTAT                                                                                                                                   <  SRR19973071.1851070/150‑1 (MQ=60)

                   GTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTC                                                                                                                                 <  SRR19973071.3194270/150‑1 (MQ=60)

                    TGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCA                                                                                                                                <  SRR19973071.3136078/150‑1 (MQ=60)

                    TGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCA                                                                                                                                <  SRR19973071.61232/150‑1 (MQ=60)

                    TGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCA                                                                                                                                <  SRR19973071.61346/150‑1 (MQ=60)

                          AAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTC                                                                                                                          >  SRR19973071.3921876/1‑150 (MQ=60)

                          AAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTC                                                                                                                          >  SRR19973071.2798178/1‑150 (MQ=60)

                          AAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTC                                                                                                                          >  SRR19973071.3921550/1‑150 (MQ=60)

                          AAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTC                                                                                                                          >  SRR19973071.4671151/1‑150 (MQ=60)

                           AATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCC                                                                                                                         <  SRR19973071.2010728/150‑1 (MQ=60)

                            ATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCC                                                                                                                        <  SRR19973071.975354/150‑1 (MQ=60)

                            ATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCC                                                                                                                        >  SRR19973071.85204/1‑150 (MQ=60)

                            ATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCC                                                                                                                        >  SRR19973071.85205/1‑150 (MQ=60)

                                 ATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACT                                                                                                                   <  SRR19973071.3933638/150‑1 (MQ=60)

                                       CGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAAT                                                                                                             >  SRR19973071.1451459/1‑150 (MQ=60)

                                       CGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAAT                                                                                                             >  SRR19973071.4769054/1‑150 (MQ=60)

                                        GTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATC                                                                                                            <  SRR19973071.1555283/150‑1 (MQ=60)

                                         TATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCT                                                                                                           <  SRR19973071.52491/150‑1 (MQ=60)

                                         TATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCT                                                                                                           <  SRR19973071.1227717/150‑1 (MQ=60)

                                          ATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTT                                                                                                          >  SRR19973071.3820241/1‑150 (MQ=60)

                                             CCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCC                                                                                                       <  SRR19973071.3850296/150‑1 (MQ=60)

                                               CCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTC                                                                                                     <  SRR19973071.3382808/150‑1 (MQ=60)

                                               CCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTCTATTCAGGCGTCCCGCACTCAAAATCTTTCCTC                                                                                                     <  SRR19973071.3382843/150‑1 (MQ=60)

                                                 CGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGC                                                                                                   >  SRR19973071.4065633/1‑150 (MQ=60)

                                                   TTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTT                                                                                                 >  SRR19973071.2227626/1‑150 (MQ=60)

                                                   TTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTT                                                                                                 <  SRR19973071.2890167/150‑1 (MQ=60)

                                                    TTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTA                                                                                                <  SRR19973071.3775448/150‑1 (MQ=60)

                                                     TCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAA                                                                                               >  SRR19973071.2578182/1‑150 (MQ=60)

                                                         GGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAA                                                                                           >  SRR19973071.1424870/1‑150 (MQ=60)

                                                           TGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACA                                                                                         >  SRR19973071.2985103/1‑150 (MQ=60)

                                                           TGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACA                                                                                         >  SRR19973071.584672/1‑150 (MQ=60)

                                                            GTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAA                                                                                        >  SRR19973071.35716/1‑150 (MQ=60)

                                                            GTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAA                                                                                        >  SRR19973071.35675/1‑150 (MQ=60)

                                                             TAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAG                                                                                       <  SRR19973071.3857099/150‑1 (MQ=60)

                                                              AAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGA                                                                                      <  SRR19973071.4313623/150‑1 (MQ=60)

                                                              AAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGA                                                                                      <  SRR19973071.4313898/150‑1 (MQ=60)

                                                              AAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGA                                                                                      <  SRR19973071.7527/150‑1 (MQ=60)

                                                              AAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGA                                                                                      >  SRR19973071.1646334/1‑150 (MQ=60)

                                                               AACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGAC                                                                                     >  SRR19973071.1204028/1‑150 (MQ=60)

                                                               AACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGAC                                                                                     <  SRR19973071.3151929/150‑1 (MQ=60)

                                                               AACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGAC                                                                                     <  SRR19973071.1715982/150‑1 (MQ=60)

                                                               AACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGAC                                                                                     <  SRR19973071.4772895/150‑1 (MQ=60)

                                                               AACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGAC                                                                                     <  SRR19973071.751743/150‑1 (MQ=60)

                                                                   GAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCT                                                                                 <  SRR19973071.3017804/150‑1 (MQ=60)

                                                                    AGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTT                                                                                <  SRR19973071.1700590/150‑1 (MQ=60)

                                                                      CCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTAT                                                                              <  SRR19973071.2915098/150‑1 (MQ=60)

                                                                      CCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTAT                                                                              >  SRR19973071.1603615/1‑150 (MQ=60)

                                                                      CCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTAT                                                                              <  SRR19973071.2915224/150‑1 (MQ=60)

                                                                        GGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCA                                                                            >  SRR19973071.4672378/1‑150 (MQ=60)

                                                                         GAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCAT                                                                           <  SRR19973071.4188077/150‑1 (MQ=60)

                                                                         GAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCAT                                                                           >  SRR19973071.1061727/1‑150 (MQ=60)

                                                                         GAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCAT                                                                           <  SRR19973071.4473888/150‑1 (MQ=60)

                                                                         GAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCAT                                                                           <  SRR19973071.4187994/150‑1 (MQ=60)

                                                                            TACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGC                                                                        <  SRR19973071.123896/150‑1 (MQ=60)

                                                                             ACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCA                                                                       >  SRR19973071.2631877/1‑150 (MQ=60)

                                                                               GTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGC                                                                     <  SRR19973071.3822209/150‑1 (MQ=60)

                                                                               GTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGC                                                                     <  SRR19973071.3350979/150‑1 (MQ=60)

                                                                               GTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGC                                                                     <  SRR19973071.3822095/150‑1 (MQ=60)

                                                                               GTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGC                                                                     <  SRR19973071.3351014/150‑1 (MQ=60)

                                                                                TTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCC                                                                    >  SRR19973071.4779356/1‑150 (MQ=60)

                                                                                 TCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCA                                                                   <  SRR19973071.2640488/150‑1 (MQ=60)

                                                                                      TATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTC                                                              >  SRR19973071.3807262/1‑150 (MQ=60)

                                                                                      TATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTC                                                              >  SRR19973071.3807344/1‑150 (MQ=60)

                                                                                       ATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCT                                                             >  SRR19973071.3799616/1‑150 (MQ=60)

                                                                                        TCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTT                                                            >  SRR19973071.3234388/1‑150 (MQ=60)

                                                                                        TCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTT                                                            >  SRR19973071.3234424/1‑150 (MQ=60)

                                                                                         CGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTG                                                           >  SRR19973071.430293/1‑150 (MQ=60)

                                                                                             TGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATAC                                                       <  SRR19973071.1065224/150‑1 (MQ=60)

                                                                                             TGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATAC                                                       <  SRR19973071.1065873/150‑1 (MQ=60)

                                                                                             TGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATAC                                                       <  SRR19973071.1065381/150‑1 (MQ=60)

                                                                                                  TGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAAT                                                  <  SRR19973071.2861324/150‑1 (MQ=60)

                                                                                                    CTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCT                                                >  SRR19973071.4777003/1‑150 (MQ=60)

                                                                                                     TACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTC                                               >  SRR19973071.1883481/1‑150 (MQ=60)

                                                                                                      ACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCT                                              >  SRR19973071.884343/1‑150 (MQ=60)

                                                                                                       CCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTT                                             <  SRR19973071.2340809/150‑1 (MQ=60)

                                                                                                       CCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTT                                             <  SRR19973071.2340842/150‑1 (MQ=60)

                                                                                                         CGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGC                                           >  SRR19973071.2511658/1‑150 (MQ=60)

                                                                                                             CAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCT                                       <  SRR19973071.3318212/150‑1 (MQ=60)

                                                                                                               GCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGG                                     <  SRR19973071.3507407/150‑1 (MQ=60)

                                                                                                                  CGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAA                                  <  SRR19973071.3290079/150‑1 (MQ=60)

                                                                                                                  CGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAA                                  <  SRR19973071.3289488/150‑1 (MQ=60)

                                                                                                                   GCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAA                                 <  SRR19973071.4692900/150‑1 (MQ=60)

                                                                                                                   GCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAA                                 >  SRR19973071.1777930/1‑150 (MQ=60)

                                                                                                                     CAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAAC                               >  SRR19973071.2245855/1‑150 (MQ=60)

                                                                                                                      AAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACG                              >  SRR19973071.4627588/1‑150 (MQ=60)

                                                                                                                        AAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTG                            >  SRR19973071.1418516/1‑150 (MQ=60)

                                                                                                                         AGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGG                           >  SRR19973071.417774/1‑150 (MQ=60)

                                                                                                                          GCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGT                          <  SRR19973071.2083564/150‑1 (MQ=60)

                                                                                                                          GCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGT                          <  SRR19973071.717141/150‑1 (MQ=60)

                                                                                                                          GCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGT                          <  SRR19973071.2083578/150‑1 (MQ=60)

                                                                                                                          GCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGT                          <  SRR19973071.2083669/150‑1 (MQ=60)

                                                                                                                           CAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTA                         <  SRR19973071.1452977/150‑1 (MQ=60)

                                                                                                                           CAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTA                         <  SRR19973071.326356/150‑1 (MQ=60)

                                                                                                                              TCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATA                      <  SRR19973071.653177/150‑1 (MQ=60)

                                                                                                                              TCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATA                      >  SRR19973071.1138466/1‑150 (MQ=60)

                                                                                                                              TCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATA                      >  SRR19973071.3669088/1‑150 (MQ=60)

                                                                                                                               CCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAG                     <  SRR19973071.2846054/150‑1 (MQ=60)

                                                                                                                               CCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAG                     <  SRR19973071.1499673/150‑1 (MQ=60)

                                                                                                                               CAAACCTATAACAACAACAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAG                     <  SRR19973071.3702195/150‑1 (MQ=60)

                                                                                                                               ACAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAG                     <  SRR19973071.3924095/150‑1 (MQ=60)

                                                                                                                                   CCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAA                 <  SRR19973071.1588209/150‑1 (MQ=60)

                                                                                                                                    CGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTTGTAATAGAGAAA                <  SRR19973071.180962/150‑1 (MQ=60)

                                                                                                                                      ATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGACTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATATAGAAAGA              >  SRR19973071.2341191/1‑150 (MQ=60)

                                                                                                                                       TAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAG             <  SRR19973071.3853300/150‑1 (MQ=60)

                                                                                                                                        AACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAGG            >  SRR19973071.3763454/1‑150 (MQ=60)

                                                                                                                                          CAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAGGTT          <  SRR19973071.2102123/150‑1 (MQ=60)

                                                                                                                                          CAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAGGTT          <  SRR19973071.3966849/150‑1 (MQ=60)

                                                                                                                                          CAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAGGTT          <  SRR19973071.2102093/150‑1 (MQ=60)

                                                                                                                                           AACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAGGTTA         >  SRR19973071.263208/1‑150 (MQ=60)

                                                                                                                                           AACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAGGTTA         >  SRR19973071.262837/1‑150 (MQ=60)

                                                                                                                                            ACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATCGAGAAAGAGGTTAA        >  SRR19973071.1541801/1‑150 (MQ=60)

                                                                                                                                             CATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAGGTTAAT       >  SRR19973071.4261803/1‑150 (MQ=60)

                                                                                                                                               TCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAGGTTAATTT     <  SRR19973071.3064495/150‑1 (MQ=60)

                                                                                                                                               TCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAGGTTAATTT     <  SRR19973071.3650661/150‑1 (MQ=60)

                                                                                                                                               TCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAGGTTAATTT     <  SRR19973071.3650741/150‑1 (MQ=60)

                                                                                                                                                  AGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAGGTTAATTTCAT  >  SRR19973071.2403462/1‑150 (MQ=60)

TTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAGGTTAATTTCAT  >  CP009273/2993065‑2993360

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`