Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F142 I0 R1 859 274.6 8723680 96.4% 8409627 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,993,211 C→A 94.2% V14L (GTG→TTG)  ygeR ← LysM domain‑containing M23 family putative peptidase; septation lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,993,2110CA94.2% 774.7 / 33.8 240V14L (GTG→TTG) ygeRLysM domain‑containing M23 family putative peptidase; septation lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (4/10);  new base A (87/139);  total (91/149)
Fisher's exact test for biased strand distribution p-value = 5.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.28e-01

Only 100 of 240 total aligned reads displayed.

CTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAGGTTAATTT  >  CP009273/2993064‑2993357
                                                                                                                                                   |                                                                                                                                                  
 ttttACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGAt                                                                                                                                                 <  2:1617131/150‑1 (MQ=255)
 ttttACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGAt                                                                                                                                                 >  1:3000950/1‑150 (MQ=255)
  tttACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATa                                                                                                                                                <  2:2499771/150‑1 (MQ=255)
  tttACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATa                                                                                                                                                <  2:3603347/150‑1 (MQ=255)
   ttACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATAc                                                                                                                                               <  2:3223905/150‑1 (MQ=255)
    tACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATAcc                                                                                                                                              >  1:3115890/1‑150 (MQ=255)
    tACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATAcc                                                                                                                                              <  1:2337400/150‑1 (MQ=255)
     aCGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATAccc                                                                                                                                             >  1:1035115/1‑150 (MQ=255)
     aCGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATAccc                                                                                                                                             >  1:758912/1‑150 (MQ=255)
       gCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCag                                                                                                                                           <  2:1348187/150‑1 (MQ=255)
         tGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCagag                                                                                                                                         >  1:3474727/1‑150 (MQ=255)
             tCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGAttt                                                                                                                                     <  1:646946/150‑1 (MQ=255)
             tCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGAttt                                                                                                                                     >  1:261845/1‑150 (MQ=255)
             tCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGAttt                                                                                                                                     <  1:1494096/150‑1 (MQ=255)
             tCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCACGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGAttt                                                                                                                                     <  2:824877/150‑1 (MQ=255)
              cccGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGAtttt                                                                                                                                    <  1:423068/150‑1 (MQ=255)
               ccGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGAttttt                                                                                                                                   >  1:240625/1‑150 (MQ=255)
                cGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGAtttttt                                                                                                                                  <  1:2863754/150‑1 (MQ=255)
                  tggtgCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTAt                                                                                                                                <  2:3232358/150‑1 (MQ=255)
                     tgCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCa                                                                                                                             >  2:2983614/1‑150 (MQ=255)
                      gcgcGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAg                                                                                                                            <  1:302990/150‑1 (MQ=255)
                      gcgcGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAg                                                                                                                            <  2:1676888/150‑1 (MQ=255)
                       cgcgAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAgg                                                                                                                           <  2:539088/150‑1 (MQ=255)
                       cgcgAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAgg                                                                                                                           <  2:3095700/150‑1 (MQ=255)
                       cgcgAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAgg                                                                                                                           >  2:4210663/1‑150 (MQ=255)
                        gcgAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGc                                                                                                                          <  2:1408628/150‑1 (MQ=255)
                        gcgAAATACGATAGAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGc                                                                                                                          <  1:2176806/150‑1 (MQ=255)
                         cgAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCg                                                                                                                         <  2:611964/150‑1 (MQ=255)
                         cgAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACACAGAGATTTTTTATTCAGGCg                                                                                                                         >  2:3572957/1‑150 (MQ=255)
                          gAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGt                                                                                                                        >  2:1187928/1‑150 (MQ=255)
                            aaTACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTcc                                                                                                                      <  1:2708509/150‑1 (MQ=255)
                             aTACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTccc                                                                                                                     <  2:4081570/150‑1 (MQ=255)
                             aTACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTGTTTATTCAGGCGTccc                                                                                                                     >  2:2966931/1‑150 (MQ=255)
                                cGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCa                                                                                                                  >  1:2744995/1‑150 (MQ=255)
                                 gATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCAc                                                                                                                 >  1:4300935/1‑150 (MQ=255)
                                  atatAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACt                                                                                                                >  1:3062620/1‑150 (MQ=255)
                                    ataGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCa                                                                                                              <  2:1755843/150‑1 (MQ=255)
                                           aTCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCtt                                                                                                       <  1:2817071/150‑1 (MQ=255)
                                             ccccccGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTc                                                                                                     <  2:149527/150‑1 (MQ=255)
                                              cccccGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTcc                                                                                                    >  1:3878364/1‑150 (MQ=255)
                                              cccccGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTcc                                                                                                    >  1:2957473/1‑150 (MQ=255)
                                                   gTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCt                                                                                               <  1:1574885/150‑1 (MQ=255)
                                                     ttCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTa                                                                                             >  1:1313445/1‑150 (MQ=255)
                                                     ttCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTa                                                                                             >  2:1328488/1‑150 (MQ=255)
                                                     ttCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTa                                                                                             >  2:2244995/1‑150 (MQ=255)
                                                      tCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTaa                                                                                            <  1:4236835/150‑1 (MQ=255)
                                                         cGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTaaaaa                                                                                         >  2:2012077/1‑150 (MQ=255)
                                                          ggTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTaaaaaa                                                                                        <  1:2194478/150‑1 (MQ=255)
                                                            tgtAGACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACa                                                                                      <  1:1482807/150‑1 (MQ=255)
                                                            tgtAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACa                                                                                      <  1:2397636/150‑1 (MQ=255)
                                                                    gAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCt                                                                              <  1:1644931/150‑1 (MQ=255)
                                                                      gCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTa                                                                            <  1:378061/150‑1 (MQ=255)
                                                                       ccGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTAt                                                                           <  2:531989/150‑1 (MQ=255)
                                                                         ggAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCa                                                                         >  1:5275/1‑150 (MQ=255)
                                                                            aTACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATag                                                                      <  2:1650748/150‑1 (MQ=255)
                                                                             tACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATagc                                                                     <  1:855936/150‑1 (MQ=255)
                                                                              aCGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATagca                                                                    <  2:2203094/150‑1 (MQ=255)
                                                                              aCGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATagca                                                                    <  2:3373618/150‑1 (MQ=255)
                                                                               cGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATagcag                                                                   >  1:1893296/1‑150 (MQ=255)
                                                                                gTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATagcagc                                                                  >  1:2080397/1‑150 (MQ=255)
                                                                                    cTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGcagccagc                                                              >  2:1453191/1‑150 (MQ=255)
                                                                                      gTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCct                                                            <  2:3918712/150‑1 (MQ=255)
                                                                                       tATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCCGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCctc                                                           <  2:2105767/150‑1 (MQ=255)
                                                                                       tATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCctc                                                           >  2:1138559/1‑150 (MQ=255)
                                                                                       tATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCctc                                                           <  2:2108025/150‑1 (MQ=255)
                                                                                          cGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTg                                                        >  1:3190990/1‑150 (MQ=255)
                                                                                          cGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTg                                                        >  1:2450150/1‑150 (MQ=255)
                                                                                           ggATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGa                                                       >  2:3837846/1‑150 (MQ=255)
                                                                                              tgatTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATAc                                                    >  1:949109/1‑150 (MQ=255)
                                                                                              tgatTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATAc                                                    >  1:2508799/1‑150 (MQ=255)
                                                                                                 tttGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCa                                                 <  1:3130193/150‑1 (MQ=255)
                                                                                                 tttGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCa                                                 <  2:1413071/150‑1 (MQ=255)
                                                                                                 tttGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCa                                                 <  1:4342646/150‑1 (MQ=255)
                                                                                                     cTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAAtct                                             >  1:1172585/1‑150 (MQ=255)
                                                                                                      tACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAAtctc                                            >  1:3977762/1‑150 (MQ=255)
                                                                                                      tACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAAtctc                                            >  2:695518/1‑150 (MQ=255)
                                                                                                        cccGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCtt                                          <  2:3447426/150‑1 (MQ=255)
                                                                                                          cGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGc                                        >  2:1976610/1‑150 (MQ=255)
                                                                                                            aaCAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCtt                                      >  2:2523394/1‑150 (MQ=255)
                                                                                                                gCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTgg                                  <  1:3864037/150‑1 (MQ=255)
                                                                                                                gCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTgg                                  <  2:4265244/150‑1 (MQ=255)
                                                                                                                 cccGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGa                                 <  2:3122608/150‑1 (MQ=255)
                                                                                                                 cccGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGa                                 <  1:2053741/150‑1 (MQ=255)
                                                                                                                 cccGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGa                                 <  1:3596462/150‑1 (MQ=255)
                                                                                                                           gCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGt                       <  1:4171770/150‑1 (MQ=255)
                                                                                                                            cAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTa                      >  2:4349516/1‑150 (MQ=255)
                                                                                                                              gTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAAt                    <  1:1188569/150‑1 (MQ=255)
                                                                                                                               tCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATa                   <  2:12213/150‑1 (MQ=255)
                                                                                                                               tCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATa                   <  2:310933/150‑1 (MQ=255)
                                                                                                                               tCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATa                   <  1:2983614/150‑1 (MQ=255)
                                                                                                                                ccAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATag                  >  2:1900357/1‑150 (MQ=255)
                                                                                                                                 cAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATaga                 <  1:1432898/150‑1 (MQ=255)
                                                                                                                                  aaCCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATagag                >  2:2843095/1‑150 (MQ=255)
                                                                                                                                   aCCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAgaga               >  1:3097168/1‑150 (MQ=255)
                                                                                                                                      gATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAag            <  1:146322/150‑1 (MQ=255)
                                                                                                                                       aTAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAaga           <  2:3305481/150‑1 (MQ=255)
                                                                                                                                       aTAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAaga           <  2:1818173/150‑1 (MQ=255)
                                                                                                                                       aTAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAaga           <  2:1819439/150‑1 (MQ=255)
                                                                                                                                          acaacaTCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAGGt        <  2:849007/150‑1 (MQ=255)
                                                                                                                                           caacaTCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTGTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAGGtt       <  2:2150446/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAGGTTAATTT  >  CP009273/2993064‑2993357

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAGGTTAATTTCAT  >  CP009273/2993064‑2993360
                                                                                                                                                   |                                                                                                                                                     
CTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGA                                                                                                                                                     <  SRR19973079.2458235/150‑1 (MQ=60)
 TTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGAT                                                                                                                                                    >  SRR19973079.3016438/1‑150 (MQ=60)
    TACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACC                                                                                                                                                 <  SRR19973079.2349541/150‑1 (MQ=60)
    TACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACC                                                                                                                                                 >  SRR19973079.3131955/1‑150 (MQ=60)
    TACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACC                                                                                                                                                 >  SRR19973079.12371/1‑150 (MQ=60)
     ACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCC                                                                                                                                                >  SRR19973079.1040858/1‑150 (MQ=60)
     ACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCC                                                                                                                                                >  SRR19973079.763089/1‑150 (MQ=60)
      CGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCA                                                                                                                                               >  SRR19973079.1657302/1‑150 (MQ=60)
         TGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAG                                                                                                                                            >  SRR19973079.3492637/1‑150 (MQ=60)
         TGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAG                                                                                                                                            >  SRR19973079.3492634/1‑150 (MQ=60)
         TGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAG                                                                                                                                            >  SRR19973079.2477951/1‑150 (MQ=60)
             TCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTT                                                                                                                                        <  SRR19973079.650603/150‑1 (MQ=60)
             TCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTT                                                                                                                                        >  SRR19973079.263520/1‑150 (MQ=60)
             TCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTT                                                                                                                                        <  SRR19973079.1502323/150‑1 (MQ=60)
              CCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTT                                                                                                                                       <  SRR19973079.425566/150‑1 (MQ=60)
               CCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTT                                                                                                                                      >  SRR19973079.242230/1‑150 (MQ=60)
                CGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTT                                                                                                                                     <  SRR19973079.2878295/150‑1 (MQ=60)
                 GTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTA                                                                                                                                    >  SRR19973079.761696/1‑150 (MQ=60)
                  TGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAATATACCCAGAGATTTTTTAT                                                                                                                                   >  SRR19973079.955273/1‑150 (MQ=60)
                  TGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAATATACCCAGAGATTTTTTAT                                                                                                                                   >  SRR19973079.3153901/1‑150 (MQ=60)
                  TGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTAT                                                                                                                                   <  SRR19973079.3082700/150‑1 (MQ=60)
                  TGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTAT                                                                                                                                   <  SRR19973079.3082696/150‑1 (MQ=60)
                  TGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTAT                                                                                                                                   <  SRR19973079.2910358/150‑1 (MQ=60)
                  TGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTAT                                                                                                                                   <  SRR19973079.2910313/150‑1 (MQ=60)
                      GCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAG                                                                                                                               <  SRR19973079.304776/150‑1 (MQ=60)
                       CGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGG                                                                                                                              >  SRR19973079.2205283/1‑150 (MQ=60)
                        GCGAAATACGATAGAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGC                                                                                                                             <  SRR19973079.2187923/150‑1 (MQ=60)
                           AAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTC                                                                                                                          >  SRR19973079.4345566/1‑150 (MQ=60)
                            AATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCC                                                                                                                         <  SRR19973079.2722519/150‑1 (MQ=60)
                              TACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCG                                                                                                                       <  SRR19973079.80400/150‑1 (MQ=60)
                              TACGATATAGCGTATCCCCCCGTTTAACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCG                                                                                                                       <  SRR19973079.3168411/150‑1 (MQ=60)
                                CGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCA                                                                                                                     >  SRR19973079.2759156/1‑150 (MQ=60)
                                 GATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCCC                                                                                                                    >  SRR19973079.1450458/1‑150 (MQ=60)
                                 GATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCAC                                                                                                                    >  SRR19973079.4322608/1‑150 (MQ=60)
                                  ATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACT                                                                                                                   >  SRR19973079.3078477/1‑150 (MQ=60)
                                          TATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCT                                                                                                           >  SRR19973079.2371690/1‑150 (MQ=60)
                                           ATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTT                                                                                                          <  SRR19973079.2831482/150‑1 (MQ=60)
                                           ATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACGCAAAATCTT                                                                                                          >  SRR19973079.2735427/1‑150 (MQ=60)
                                              CCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCC                                                                                                       >  SRR19973079.2972651/1‑150 (MQ=60)
                                              CCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCC                                                                                                       >  SRR19973079.3898079/1‑150 (MQ=60)
                                                CCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTC                                                                                                     >  SRR19973079.3138690/1‑150 (MQ=60)
                                                   GTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCT                                                                                                  <  SRR19973079.1583577/150‑1 (MQ=60)
                                                    TTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTT                                                                                                 >  SRR19973079.253183/1‑150 (MQ=60)
                                                     TTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTA                                                                                                >  SRR19973079.1320870/1‑150 (MQ=60)
                                                      TCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAA                                                                                               >  SRR19973079.2801341/1‑150 (MQ=60)
                                                      TCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAA                                                                                               <  SRR19973079.4258270/150‑1 (MQ=60)
                                                       CACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAA                                                                                              >  SRR19973079.1909940/1‑150 (MQ=60)
                                                        ACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAA                                                                                             <  SRR19973079.3694851/150‑1 (MQ=60)
                                                         CGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAA                                                                                            >  SRR19973079.3014076/1‑150 (MQ=60)
                                                          GGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAA                                                                                           <  SRR19973079.2076911/150‑1 (MQ=60)
                                                          GGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAA                                                                                           <  SRR19973079.4275191/150‑1 (MQ=60)
                                                          GGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAA                                                                                           <  SRR19973079.2205767/150‑1 (MQ=60)
                                                           GTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAAC                                                                                          <  SRR19973079.271741/150‑1 (MQ=60)
                                                            TGTAGACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACA                                                                                         <  SRR19973079.1490924/150‑1 (MQ=60)
                                                            TGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACA                                                                                         <  SRR19973079.1491135/150‑1 (MQ=60)
                                                            TGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACA                                                                                         <  SRR19973079.2410024/150‑1 (MQ=60)
                                                              TAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATTTTTCCTCGCTTAAAAAACAAG                                                                                       <  SRR19973079.817405/150‑1 (MQ=60)
                                                                    GAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCT                                                                                 <  SRR19973079.1653730/150‑1 (MQ=60)
                                                                    GAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCT                                                                                 <  SRR19973079.1653888/150‑1 (MQ=60)
                                                                     AGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTT                                                                                <  SRR19973079.2121591/150‑1 (MQ=60)
                                                                      GCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTA                                                                               <  SRR19973079.380174/150‑1 (MQ=60)
                                                                      GCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTA                                                                               <  SRR19973079.2343376/150‑1 (MQ=60)
                                                                      GCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTA                                                                               <  SRR19973079.1302588/150‑1 (MQ=60)
                                                                      GCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTA                                                                               <  SRR19973079.2343447/150‑1 (MQ=60)
                                                                         GGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCA                                                                            <  SRR19973079.4293581/150‑1 (MQ=60)
                                                                         GGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCA                                                                            >  SRR19973079.135951/1‑150 (MQ=60)
                                                                         GGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCA                                                                            <  SRR19973079.3888394/150‑1 (MQ=60)
                                                                         GGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCA                                                                            >  SRR19973079.49346/1‑150 (MQ=60)
                                                                         GGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCA                                                                            >  SRR19973079.5347/1‑150 (MQ=60)
                                                                             TACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGC                                                                        <  SRR19973079.860882/150‑1 (MQ=60)
                                                                              ACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCA                                                                       >  SRR19973079.2997942/1‑150 (MQ=60)
                                                                               CGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAG                                                                      >  SRR19973079.1903391/1‑150 (MQ=60)
                                                                                GTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGC                                                                     >  SRR19973079.2091263/1‑150 (MQ=60)
                                                                                      GTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCT                                                               <  SRR19973079.3025382/150‑1 (MQ=60)
                                                                                      GTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCT                                                               >  SRR19973079.285361/1‑150 (MQ=60)
                                                                                         TCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTT                                                            <  SRR19973079.4091362/150‑1 (MQ=60)
                                                                                          CGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTG                                                           >  SRR19973079.2462720/1‑150 (MQ=60)
                                                                                          CGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTG                                                           <  SRR19973079.1683734/150‑1 (MQ=60)
                                                                                          CGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTG                                                           >  SRR19973079.2462723/1‑150 (MQ=60)
                                                                                          CGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTG                                                           >  SRR19973079.3207288/1‑150 (MQ=60)
                                                                                          CGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTG                                                           <  SRR19973079.4173900/150‑1 (MQ=60)
                                                                                            GATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGAT                                                         >  SRR19973079.4370996/1‑150 (MQ=60)
                                                                                              TGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATAC                                                       >  SRR19973079.954547/1‑150 (MQ=60)
                                                                                              TGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATAC                                                       >  SRR19973079.2521514/1‑150 (MQ=60)
                                                                                                 TTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCA                                                    <  SRR19973079.3146303/150‑1 (MQ=60)
                                                                                                 TTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCA                                                    <  SRR19973079.4364432/150‑1 (MQ=60)
                                                                                                     CTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCT                                                >  SRR19973079.1179341/1‑150 (MQ=60)
                                                                                                      TACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTC                                               >  SRR19973079.3997759/1‑150 (MQ=60)
                                                                                                       ACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCT                                              >  SRR19973079.1582365/1‑150 (MQ=60)
                                                                                                       ACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCT                                              <  SRR19973079.3071408/150‑1 (MQ=60)
                                                                                                       ACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCT                                              <  SRR19973079.589221/150‑1 (MQ=60)
                                                                                                              CAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCT                                       >  SRR19973079.3885436/1‑150 (MQ=60)
                                                                                                                GCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGG                                     <  SRR19973079.3883711/150‑1 (MQ=60)
                                                                                                                 cgcgcgaaatgcattccataCGATAACAACATCAAGATACCCTGAGTTTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCATCTCTTAAAAAACAATACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTTCTTCTGGA                                    <  SRR19973079.3575013/130‑1 (MQ=60)
                                                                                                                 CCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGA                                    <  SRR19973079.2064510/150‑1 (MQ=60)
                                                                                                                 CCCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGA                                    <  SRR19973079.3614735/150‑1 (MQ=60)
                                                                                                                  CCGCCAAAAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAA                                   <  SRR19973079.4231983/150‑1 (MQ=60)
                                                                                                                         AAGCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTG                            <  SRR19973079.2777724/150‑1 (MQ=60)
                                                                                                                           GCAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGT                          <  SRR19973079.4192923/150‑1 (MQ=60)
                                                                                                                            CAGTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTA                         <  SRR19973079.3950317/150‑1 (MQ=60)
                                                                                                                              GTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAAT                       <  SRR19973079.1195420/150‑1 (MQ=60)
                                                                                                                              GTCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAAT                       <  SRR19973079.1194770/150‑1 (MQ=60)
                                                                                                                               TCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATA                      <  SRR19973079.3029338/150‑1 (MQ=60)
                                                                                                                               TCCAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATA                      >  SRR19973079.3422383/1‑150 (MQ=60)
                                                                                                                               TCCAACCGATAACAACATCAAGAGACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATA                      <  SRR19973079.2998982/150‑1 (MQ=60)
                                                                                                                                 CAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGA                    <  SRR19973079.385389/150‑1 (MQ=60)
                                                                                                                                 CAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGA                    <  SRR19973079.1440640/150‑1 (MQ=60)
                                                                                                                                 CAACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGA                    <  SRR19973079.1440761/150‑1 (MQ=60)
                                                                                                                                   ACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGA                  >  SRR19973079.3114942/1‑150 (MQ=60)
                                                                                                                                   ACCGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGA                  >  SRR19973079.3113167/1‑150 (MQ=60)
                                                                                                                                     CGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAA                <  SRR19973079.425814/150‑1 (MQ=60)
                                                                                                                                     CGATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAA                <  SRR19973079.2610978/150‑1 (MQ=60)
                                                                                                                                      TATAACAACATAAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAG               <  SRR19973079.147508/150‑1 (MQ=60)
                                                                                                                                      GATAACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAG               <  SRR19973079.147476/150‑1 (MQ=60)
                                                                                                                                         AACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAGG            <  SRR19973079.998177/150‑1 (MQ=60)
                                                                                                                                         AACAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAGG            >  SRR19973079.1532804/1‑150 (MQ=60)
                                                                                                                                           CAACATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAGGTT          <  SRR19973079.242098/150‑1 (MQ=60)
                                                                                                                                              CATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAGGTTAAT       >  SRR19973079.2161985/1‑150 (MQ=60)
                                                                                                                                               ATCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAGGTTAATT      <  SRR19973079.1145081/150‑1 (MQ=60)
                                                                                                                                                TCAAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAGGTTAATTT     <  SRR19973079.943067/150‑1 (MQ=60)
                                                                                                                                                  AAGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAGGTTAATTTCA   <  SRR19973079.4152956/150‑1 (MQ=60)
                                                                                                                                                   AGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAGGTTAATTTCAT  >  SRR19973079.4340418/1‑150 (MQ=60)
                                                                                                                                                   AGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAGGTTAATTTCAT  >  SRR19973079.4340235/1‑150 (MQ=60)
                                                                                                                                                   AGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAGGTTAATTTCAT  >  SRR19973079.1921743/1‑150 (MQ=60)
                                                                                                                                                   AGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAGGTTAATTTCAT  >  SRR19973079.1920472/1‑150 (MQ=60)
                                                                                                                                                   |                                                                                                                                                     
CTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCCGCACTCAAAATCTTTCCTCGCTTAAAAAACAAGACGCCTTATCATAGCAGCCAGCCTCTTGATACCCAATCTCTTGCTTCTGGAAAAACGTGGTAATAGAGAAAGAGGTTAATTTCAT  >  CP009273/2993064‑2993360

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑