Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F121 I0 R1 856 273.6 9081716 94.1% 8545894 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,488,966 G→C 90.5% A533P (GCC→CCC)  nirB → nitrite reductase, large subunit, NAD(P)H‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,488,9660GC90.5% 874.5 / 79.8 243A533P (GCC→CCC) nirBnitrite reductase, large subunit, NAD(P)H‑binding
Reads supporting (aligned to +/- strand):  ref base G (16/7);  new base C (126/94);  total (142/101)
Fisher's exact test for biased strand distribution p-value = 2.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01

Only 100 of 247 total aligned reads displayed.

AACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGAAGGGCTG  >  CP009273/3488819‑3489112
                                                                                                                                                   |                                                                                                                                                  
aacCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCAGCTGccc                                                                                                                                                  <  1:2190950/150‑1 (MQ=255)
  ccTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCcctc                                                                                                                                                >  1:1453565/1‑150 (MQ=255)
    tgtgCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGGcctcct                                                                                                                                              >  1:2086789/1‑150 (MQ=255)
     gtgCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCctg                                                                                                                                             >  1:3223256/1‑150 (MQ=255)
       gCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGGCCTCctgct                                                                                                                                           >  2:753402/1‑150 (MQ=255)
       gCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCctgct                                                                                                                                           >  2:580600/1‑150 (MQ=255)
           acacTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGaa                                                                                                                                       >  2:2243586/1‑150 (MQ=255)
              cTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGa                                                                                                                                    >  2:4302673/1‑150 (MQ=255)
              cTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGa                                                                                                                                    >  1:4002128/1‑150 (MQ=255)
               tttGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGaa                                                                                                                                   >  2:1805290/1‑150 (MQ=255)
                  gCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATAc                                                                                                                                >  1:1618660/1‑150 (MQ=255)
                  gCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATAc                                                                                                                                >  1:1616602/1‑150 (MQ=255)
                     tATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACAtt                                                                                                                             <  1:2338743/150‑1 (MQ=255)
                     tATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACAtt                                                                                                                             >  1:1318933/1‑150 (MQ=255)
                      aTTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTc                                                                                                                            >  1:3075726/1‑150 (MQ=255)
                       ttCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCt                                                                                                                           >  1:1504342/1‑150 (MQ=255)
                             tCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCAGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGcc                                                                                                                     >  1:3294865/1‑150 (MQ=255)
                              cAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCAGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCg                                                                                                                    >  2:4459411/1‑150 (MQ=255)
                               aGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCAgg                                                                                                                   >  1:2037920/1‑150 (MQ=255)
                                ggAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGa                                                                                                                  >  2:48057/1‑150 (MQ=255)
                                ggAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGa                                                                                                                  <  2:4033212/150‑1 (MQ=255)
                                  aaCTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAAc                                                                                                                <  2:2822056/150‑1 (MQ=255)
                                      gTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCAGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATAc                                                                                                            <  1:4388668/150‑1 (MQ=255)
                                       ttCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACt                                                                                                           >  2:2667799/1‑150 (MQ=255)
                                       ttCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACt                                                                                                           >  2:4535297/1‑150 (MQ=255)
                                       ttCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGGTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACt                                                                                                           >  2:628869/1‑150 (MQ=255)
                                         ccATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTcc                                                                                                         <  2:3832258/150‑1 (MQ=255)
                                          cATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCg                                                                                                        >  2:601911/1‑150 (MQ=255)
                                          cATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCg                                                                                                        >  2:2863458/1‑150 (MQ=255)
                                             ttGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTg                                                                                                     <  2:1384145/150‑1 (MQ=255)
                                               gATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCAGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCa                                                                                                   <  1:3998116/150‑1 (MQ=255)
                                                aTCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAg                                                                                                  >  2:1991666/1‑150 (MQ=255)
                                                aTCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAg                                                                                                  <  1:3123828/150‑1 (MQ=255)
                                                aTCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAg                                                                                                  <  1:478581/150‑1 (MQ=255)
                                                aTCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAg                                                                                                  >  2:4168450/1‑150 (MQ=255)
                                                   cgcgTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGAt                                                                                               >  1:3969098/1‑150 (MQ=255)
                                                    gcgTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGAtt                                                                                              <  2:3117752/150‑1 (MQ=255)
                                                     cgTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTc                                                                                             >  2:2160780/1‑150 (MQ=255)
                                                     cgTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCAGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTc                                                                                             <  2:1250896/150‑1 (MQ=255)
                                                      gTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCt                                                                                            <  2:14198/150‑1 (MQ=255)
                                                      gTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCt                                                                                            <  2:3281545/150‑1 (MQ=255)
                                                       ttGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTa                                                                                           >  1:3360288/1‑150 (MQ=255)
                                                       ttGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTa                                                                                           <  2:121147/150‑1 (MQ=255)
                                                         gAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCAGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAAc                                                                                         >  2:786007/1‑150 (MQ=255)
                                                          aaGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACg                                                                                        >  1:617515/1‑150 (MQ=255)
                                                            ggCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCAGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGAc                                                                                      >  1:649002/1‑150 (MQ=255)
                                                               aTTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAAc                                                                                   <  2:3008154/150‑1 (MQ=255)
                                                               aTTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCAGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAAc                                                                                   <  2:2880399/150‑1 (MQ=255)
                                                                ttAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACt                                                                                  <  2:1888584/150‑1 (MQ=255)
                                                                 tAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACtt                                                                                 >  2:2086526/1‑150 (MQ=255)
                                                                   aaaCCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCAGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTcc                                                                               >  1:1952943/1‑150 (MQ=255)
                                                                    aaCCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCt                                                                              <  1:4206802/150‑1 (MQ=255)
                                                                     aCCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGACTACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTc                                                                             >  1:3702376/1‑150 (MQ=255)
                                                                     aCCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTc                                                                             >  1:2009175/1‑150 (MQ=255)
                                                                          cGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTaa                                                                        >  2:1100976/1‑150 (MQ=255)
                                                                           gaagaaCTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAAc                                                                       <  1:3750424/150‑1 (MQ=255)
                                                                           gaagaaCTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAAc                                                                       >  1:3426729/1‑150 (MQ=255)
                                                                             agaaCTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACAt                                                                     <  1:114581/150‑1 (MQ=255)
                                                                               aaCTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATcc                                                                   >  1:1191957/1‑150 (MQ=255)
                                                                                aCTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCa                                                                  >  1:4120601/1‑150 (MQ=255)
                                                                                aCTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCa                                                                  <  2:2628707/150‑1 (MQ=255)
                                                                                 ctgctgGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAg                                                                 >  1:1852695/1‑150 (MQ=255)
                                                                                    ctgGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGaaa                                                              >  1:760951/1‑150 (MQ=255)
                                                                                       gCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCAGCTGCCCTCCTGCTGGAACTAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGAc                                                           <  1:875382/150‑1 (MQ=255)
                                                                                        cGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACg                                                          >  2:2164711/1‑150 (MQ=255)
                                                                                        cGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCAGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACg                                                          >  2:1743117/1‑150 (MQ=255)
                                                                                         gAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACgg                                                         >  2:2404328/1‑150 (MQ=255)
                                                                                           aaCACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCa                                                       <  2:4341786/150‑1 (MQ=255)
                                                                                           aaCACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCa                                                       <  2:3498466/150‑1 (MQ=255)
                                                                                            acacGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCTGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCAc                                                      <  1:3478348/150‑1 (MQ=255)
                                                                                             cacGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCAGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGTAACATACTCCGCTGCTGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCAcc                                                     <  1:986835/150‑1 (MQ=255)
                                                                                              acGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCt                                                    <  2:2833962/150‑1 (MQ=255)
                                                                                              acGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCAGCTGCCCTCCTGCTGGAACGAATAAATTCTGAAGCCGGAACATACTCCGCTGCAGGATTATAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCt                                                    <  1:826176/150‑1 (MQ=255)
                                                                                               cGGCAAACGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTa                                                   >  2:2702435/1‑150 (MQ=255)
                                                                                                 gCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACt                                                 <  1:4214532/150‑1 (MQ=255)
                                                                                                  cAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTc                                                >  1:1664866/1‑150 (MQ=255)
                                                                                                  cAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTc                                                >  2:2805685/1‑150 (MQ=255)
                                                                                                           cGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATccc                                       <  2:1651347/150‑1 (MQ=255)
                                                                                                              ttGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCcgcg                                    >  2:1873540/1‑150 (MQ=255)
                                                                                                               tgtgAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGt                                   >  1:1043769/1‑150 (MQ=255)
                                                                                                                 tgAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTtc                                 >  1:2489788/1‑150 (MQ=255)
                                                                                                                 tgAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTtc                                 <  2:3979501/150‑1 (MQ=255)
                                                                                                                   aaGTTTGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTtctc                               >  2:572524/1‑150 (MQ=255)
                                                                                                                     gTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCg                             >  2:3700443/1‑150 (MQ=255)
                                                                                                                          tAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCgg                        >  1:2870536/1‑150 (MQ=255)
                                                                                                                            aaccaaccGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTg                      <  2:559909/150‑1 (MQ=255)
                                                                                                                             accaaccGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGa                     >  1:1627684/1‑150 (MQ=255)
                                                                                                                             accaaccGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGa                     >  1:4083734/1‑150 (MQ=255)
                                                                                                                               caaccGTCGGTTCGCAGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCACGCGTTCTCCGGGCGGTGaaa                   >  1:1898279/1‑150 (MQ=255)
                                                                                                                                aaccGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAAt                  >  1:1214419/1‑150 (MQ=255)
                                                                                                                                 accGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATc                 <  2:3902778/150‑1 (MQ=255)
                                                                                                                                  ccGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCa                <  1:639354/150‑1 (MQ=255)
                                                                                                                                   cGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCAc               <  2:1071097/150‑1 (MQ=255)
                                                                                                                                    gTCGGTTCGCAGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCAcc              <  2:3430322/150‑1 (MQ=255)
                                                                                                                                       ggTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCg           >  2:3344557/1‑150 (MQ=255)
                                                                                                                                       ggTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCg           <  2:3070346/150‑1 (MQ=255)
                                                                                                                                       ggTTCGCAGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCg           >  2:155583/1‑150 (MQ=255)
                                                                                                                                           cGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCGGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGAAg       <  1:3034355/150‑1 (MQ=255)
                                                                                                                                            gctgctGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGAAgg      <  2:821733/150‑1 (MQ=255)
                                                                                                                                             ctgctgCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGAAggg     <  1:3160814/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGAAGGGCTG  >  CP009273/3488819‑3489112

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACAACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGAAGGGCTGAT  >  CP009273/3488817‑3489114
                                                                                                                                                     |                                                                                                                                                    
ACAACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGC                                                                                                                                                      >  SRR19973080.466142/1‑150 (MQ=60)
 CAACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCC                                                                                                                                                     >  SRR19973080.84015/1‑150 (MQ=60)
  AACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCAGCTGCCC                                                                                                                                                    <  SRR19973080.2203125/150‑1 (MQ=60)
    CCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTC                                                                                                                                                  >  SRR19973080.1461990/1‑150 (MQ=60)
      TGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCT                                                                                                                                                >  SRR19973080.2098589/1‑150 (MQ=60)
       GTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTG                                                                                                                                               >  SRR19973080.3241034/1‑150 (MQ=60)
       GTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTG                                                                                                                                               >  SRR19973080.3241030/1‑150 (MQ=60)
       GTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTG                                                                                                                                               >  SRR19973080.1915568/1‑150 (MQ=60)
            AACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGA                                                                                                                                          >  SRR19973080.156824/1‑150 (MQ=60)
               ACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACG                                                                                                                                       <  SRR19973080.957961/150‑1 (MQ=60)
                CTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGA                                                                                                                                      >  SRR19973080.2068748/1‑150 (MQ=60)
                CTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGA                                                                                                                                      >  SRR19973080.4023978/1‑150 (MQ=60)
                CTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGA                                                                                                                                      >  SRR19973080.4024109/1‑150 (MQ=60)
                  TTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAAT                                                                                                                                    >  SRR19973080.3747820/1‑150 (MQ=60)
                   TGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATA                                                                                                                                   >  SRR19973080.826624/1‑150 (MQ=60)
                    GCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATAC                                                                                                                                  >  SRR19973080.1626061/1‑150 (MQ=60)
                    GCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATAC                                                                                                                                  >  SRR19973080.1626200/1‑150 (MQ=60)
                    GCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATAC                                                                                                                                  >  SRR19973080.1628131/1‑150 (MQ=60)
                     CTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCAGCTGCCCTCCTGCTGGAACGAATACA                                                                                                                                 >  SRR19973080.2381834/1‑150 (MQ=60)
                      TTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACAT                                                                                                                                <  SRR19973080.3841064/150‑1 (MQ=60)
                       TATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATT                                                                                                                               <  SRR19973080.2351747/150‑1 (MQ=60)
                       TATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATT                                                                                                                               >  SRR19973080.1326909/1‑150 (MQ=60)
                       TATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATT                                                                                                                               >  SRR19973080.1326192/1‑150 (MQ=60)
                        ATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTC                                                                                                                              >  SRR19973080.3092497/1‑150 (MQ=60)
                        ATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTC                                                                                                                              <  SRR19973080.4338836/150‑1 (MQ=60)
                         TTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCT                                                                                                                             >  SRR19973080.1512911/1‑150 (MQ=60)
                             CGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCAGCTGCCCTCCTGCTGGAACGAATACATTCTGAAG                                                                                                                         <  SRR19973080.547681/150‑1 (MQ=60)
                              GTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGC                                                                                                                        >  SRR19973080.2578384/1‑150 (MQ=60)
                               TCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCAGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCC                                                                                                                       >  SRR19973080.3312919/1‑150 (MQ=60)
                                CAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCAGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCG                                                                                                                      >  SRR19973080.3990664/1‑150 (MQ=60)
                                 AGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGG                                                                                                                     >  SRR19973080.2049488/1‑150 (MQ=60)
                                 AGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCAGG                                                                                                                     >  SRR19973080.2049496/1‑150 (MQ=60)
                                    AACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCAGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAAC                                                                                                                  <  SRR19973080.503274/150‑1 (MQ=60)
                                     ACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACA                                                                                                                 <  SRR19973080.3326407/150‑1 (MQ=60)
                                        GTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCAGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATAC                                                                                                              <  SRR19973080.4412582/150‑1 (MQ=60)
                                            CATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCG                                                                                                          >  SRR19973080.3620057/1‑150 (MQ=60)
                                              TTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCT                                                                                                        <  SRR19973080.1551030/150‑1 (MQ=60)
                                               TTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTG                                                                                                       >  SRR19973080.2036781/1‑150 (MQ=60)
                                               TTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTG                                                                                                       >  SRR19973080.4043558/1‑150 (MQ=60)
                                                TGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGC                                                                                                      >  SRR19973080.4354845/1‑150 (MQ=60)
                                                 GATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAATCCGGAACATACTCCGCTGCA                                                                                                     >  SRR19973080.3087046/1‑150 (MQ=60)
                                                 GATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCA                                                                                                     >  SRR19973080.3087075/1‑150 (MQ=60)
                                                 GATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCA                                                                                                     >  SRR19973080.2925043/1‑150 (MQ=60)
                                                 GATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCAGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCA                                                                                                     <  SRR19973080.4020080/150‑1 (MQ=60)
                                                  ATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAG                                                                                                    <  SRR19973080.3140999/150‑1 (MQ=60)
                                                  ATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAG                                                                                                    <  SRR19973080.3141010/150‑1 (MQ=60)
                                                  ATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAG                                                                                                    <  SRR19973080.481627/150‑1 (MQ=60)
                                                    CCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGA                                                                                                  >  SRR19973080.1460920/1‑150 (MQ=60)
                                                     CGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGAT                                                                                                 >  SRR19973080.3990946/1‑150 (MQ=60)
                                                     CGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCAGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGAT                                                                                                 >  SRR19973080.1472943/1‑150 (MQ=60)
                                                         TTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTA                                                                                             >  SRR19973080.3378526/1‑150 (MQ=60)
                                                          TGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAA                                                                                            <  SRR19973080.3195011/150‑1 (MQ=60)
                                                           GAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAGGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAAC                                                                                           >  SRR19973080.1179765/1‑150 (MQ=60)
                                                           GAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAAC                                                                                           <  SRR19973080.639855/150‑1 (MQ=60)
                                                            AAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACG                                                                                          >  SRR19973080.621307/1‑150 (MQ=60)
                                                              GGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCAGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGAC                                                                                        >  SRR19973080.652923/1‑150 (MQ=60)
                                                                     AAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCAGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCC                                                                                 >  SRR19973080.1964015/1‑150 (MQ=60)
                                                                      AACCTTCGAAGAACTGCTGGCGAAACACGGCAGAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCT                                                                                <  SRR19973080.4226856/150‑1 (MQ=60)
                                                                      AACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCT                                                                                <  SRR19973080.4229672/150‑1 (MQ=60)
                                                                       ACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGACTACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTC                                                                               >  SRR19973080.3722706/1‑150 (MQ=60)
                                                                       ACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTC                                                                               >  SRR19973080.2020601/1‑150 (MQ=60)
                                                                        CCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCAGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCG                                                                              >  SRR19973080.2142209/1‑150 (MQ=60)
                                                                        CCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCAGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCG                                                                              >  SRR19973080.4504071/1‑150 (MQ=60)
                                                                         CTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCAGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGC                                                                             >  SRR19973080.983201/1‑150 (MQ=60)
                                                                         CTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCAGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGC                                                                             >  SRR19973080.3267156/1‑150 (MQ=60)
                                                                          TTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCT                                                                            >  SRR19973080.1751263/1‑150 (MQ=60)
                                                                             GAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAAC                                                                         <  SRR19973080.3770902/150‑1 (MQ=60)
                                                                             GAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAAC                                                                         >  SRR19973080.3445367/1‑150 (MQ=60)
                                                                              AAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACA                                                                        >  SRR19973080.1144496/1‑150 (MQ=60)
                                                                               AGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACAT                                                                       >  SRR19973080.342500/1‑150 (MQ=60)
                                                                               AGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACAT                                                                       <  SRR19973080.115572/150‑1 (MQ=60)
                                                                               AGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACAT                                                                       >  SRR19973080.342513/1‑150 (MQ=60)
                                                                                 AACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCC                                                                     >  SRR19973080.1199129/1‑150 (MQ=60)
                                                                                  ACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGTAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCA                                                                    <  SRR19973080.2306584/150‑1 (MQ=60)
                                                                                  ACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCA                                                                    >  SRR19973080.4142952/1‑150 (MQ=60)
                                                                                   CTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAG                                                                   >  SRR19973080.1863087/1‑150 (MQ=60)
                                                                                   CTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAG                                                                   >  SRR19973080.1863723/1‑150 (MQ=60)
                                                                                   CTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAG                                                                   <  SRR19973080.2265342/150‑1 (MQ=60)
                                                                                      CTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAA                                                                >  SRR19973080.765222/1‑150 (MQ=60)
                                                                                       TGGCGAAACACGTCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGTAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAG                                                               <  SRR19973080.1216493/150‑1 (MQ=60)
                                                                                       TGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACTTTCTGAAGCCGTAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAG                                                               <  SRR19973080.1216614/150‑1 (MQ=60)
                                                                                         GCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCAGCTGCCCTCCTGCTGGAACTAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGAC                                                             <  SRR19973080.880734/150‑1 (MQ=60)
                                                                                         GCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCAGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGAC                                                             <  SRR19973080.880729/150‑1 (MQ=60)
                                                                                              ACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCTGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCAC                                                        <  SRR19973080.3497479/150‑1 (MQ=60)
                                                                                              ACACGGCAAAGGATACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGAAGTATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCAC                                                        <  SRR19973080.3497288/150‑1 (MQ=60)
                                                                                               CACGGCAAAGGATACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCAGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGTAACATACTCCGCTGCTGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACC                                                       <  SRR19973080.992780/150‑1 (MQ=60)
                                                                                                ACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCAGCTGCCCTCCTGCTGGAACGAATAAATTCTGAAGCCGGAACATACTCCGCTGCAGGATTATAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCT                                                      <  SRR19973080.831103/150‑1 (MQ=60)
                                                                                                   GCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACT                                                   <  SRR19973080.4237456/150‑1 (MQ=60)
                                                                                                    CAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTC                                                  >  SRR19973080.1672816/1‑150 (MQ=60)
                                                                                                    CAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTC                                                  >  SRR19973080.1674590/1‑150 (MQ=60)
                                                                                                       AGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGT                                               >  SRR19973080.4007097/1‑150 (MQ=60)
                                                                                                           TACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATC                                           >  SRR19973080.19758/1‑150 (MQ=60)
                                                                                                             CGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCAGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCTGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCC                                         <  SRR19973080.3690860/150‑1 (MQ=60)
                                                                                                                 TGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGT                                     >  SRR19973080.1049925/1‑150 (MQ=60)
                                                                                                                   TGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTC                                   >  SRR19973080.3157572/1‑150 (MQ=60)
                                                                                                                   TGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTC                                   >  SRR19973080.2503536/1‑150 (MQ=60)
                                                                                                                   TGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTC                                   >  SRR19973080.2503800/1‑150 (MQ=60)
                                                                                                                    GAAGTTTGTAAACCAACCGTCGGTTCGCAGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCT                                  <  SRR19973080.801098/150‑1 (MQ=60)
                                                                                                                            TAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGG                          >  SRR19973080.2886257/1‑150 (MQ=60)
                                                                                                                             AAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGT                         >  SRR19973080.4107384/1‑150 (MQ=60)
                                                                                                                               ACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGA                       >  SRR19973080.1637217/1‑150 (MQ=60)
                                                                                                                               ACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGA                       >  SRR19973080.4105987/1‑150 (MQ=60)
                                                                                                                               ACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGA                       >  SRR19973080.4105837/1‑150 (MQ=60)
                                                                                                                                 CAACCGTCGGTTCGCAGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAA                     >  SRR19973080.4198152/1‑150 (MQ=60)
                                                                                                                                 CAACCGTCGGTTCGCAGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAA                     >  SRR19973080.764295/1‑150 (MQ=60)
                                                                                                                                 CAACCGTCGGTTCGCAGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCACGCGTTCTCCGGGCGGTGAAA                     >  SRR19973080.1908907/1‑150 (MQ=60)
                                                                                                                                  AACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAAT                    >  SRR19973080.1221762/1‑150 (MQ=60)
                                                                                                                                    CCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCA                  <  SRR19973080.643239/150‑1 (MQ=60)
                                                                                                                                    CCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCA                  <  SRR19973080.643643/150‑1 (MQ=60)
                                                                                                                                    CCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCA                  <  SRR19973080.643853/150‑1 (MQ=60)
                                                                                                                                        CGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCC              >  SRR19973080.1947843/1‑150 (MQ=60)
                                                                                                                                         GGTTCGCAGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCG             >  SRR19973080.1836210/1‑150 (MQ=60)
                                                                                                                                             CGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCtgcattt         >  SRR19973080.1633297/1‑143 (MQ=60)
                                                                                                                                             CGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCtgcattt         >  SRR19973080.1633310/1‑143 (MQ=60)
                                                                                                                                             CGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCGGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGAAG         <  SRR19973080.3050718/150‑1 (MQ=60)
                                                                                                                                              GCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGAAGG        <  SRR19973080.1405760/150‑1 (MQ=60)
                                                                                                                                               CTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGAAGGG       <  SRR19973080.3178211/150‑1 (MQ=60)
                                                                                                                                               CTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGAAGGG       <  SRR19973080.3178244/150‑1 (MQ=60)
                                                                                                                                                  CTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGACAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGAAGGGCTG    >  SRR19973080.4229963/1‑150 (MQ=60)
                                                                                                                                                   TGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGAAGGGCTGA   <  SRR19973080.3250610/150‑1 (MQ=60)
                                                                                                                                                    GCCCTCCTGCTGGAACGAATACATTCTTAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCGACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGAAGGGCTGAT  <  SRR19973080.3588630/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
ACAACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGAAGGGCTGAT  >  CP009273/3488817‑3489114

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑