Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F142 I0 R1 859 274.6 8723680 96.4% 8409627 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,488,966 G→C 91.1% A533P (GCC→CCC)  nirB → nitrite reductase, large subunit, NAD(P)H‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,488,9660GC91.1% 874.6 / 69.8 237A533P (GCC→CCC) nirBnitrite reductase, large subunit, NAD(P)H‑binding
Reads supporting (aligned to +/- strand):  ref base G (14/7);  new base C (110/106);  total (124/113)
Fisher's exact test for biased strand distribution p-value = 1.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 238 total aligned reads displayed.

ACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGAAGGGCTG  >  CP009273/3488820‑3489112
                                                                                                                                                  |                                                                                                                                                  
acCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCcct                                                                                                                                                 >  2:4096432/1‑150 (MQ=255)
  cTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCcctcc                                                                                                                                               >  1:3930877/1‑150 (MQ=255)
   tgtgCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCcctcct                                                                                                                                              <  1:1954126/150‑1 (MQ=255)
    gtgCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCctg                                                                                                                                             <  2:1900861/150‑1 (MQ=255)
    gtgCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCctg                                                                                                                                             <  1:1784828/150‑1 (MQ=255)
       cGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCGTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGGCCTCctgctg                                                                                                                                          >  2:3227383/1‑150 (MQ=255)
        gAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTgg                                                                                                                                         <  1:2221252/150‑1 (MQ=255)
        gAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTgg                                                                                                                                         >  1:3389059/1‑150 (MQ=255)
           cacTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAAc                                                                                                                                      >  2:3343682/1‑150 (MQ=255)
           cacTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAAc                                                                                                                                      >  2:1344729/1‑150 (MQ=255)
            acTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACg                                                                                                                                     <  2:3568079/150‑1 (MQ=255)
            acTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACg                                                                                                                                     >  2:3033233/1‑150 (MQ=255)
              tttGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGaa                                                                                                                                   >  1:292756/1‑150 (MQ=255)
                 gCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATAc                                                                                                                                <  2:742497/150‑1 (MQ=255)
                   ttATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACAt                                                                                                                              <  2:3186958/150‑1 (MQ=255)
                    tATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTCCGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACAtt                                                                                                                             >  1:1162090/1‑150 (MQ=255)
                    tATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACAtt                                                                                                                             <  2:2388807/150‑1 (MQ=255)
                        cgcgTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGa                                                                                                                         >  1:1392929/1‑150 (MQ=255)
                           gTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGc                                                                                                                      >  1:3637663/1‑150 (MQ=255)
                           gTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTGTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGc                                                                                                                      >  2:2670876/1‑150 (MQ=255)
                            tCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGcc                                                                                                                     <  1:1676263/150‑1 (MQ=255)
                            tCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGcc                                                                                                                     <  2:3333713/150‑1 (MQ=255)
                             cAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCg                                                                                                                    <  2:2429968/150‑1 (MQ=255)
                              aGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCCAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCgg                                                                                                                   >  2:594819/1‑150 (MQ=255)
                              aGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCgg                                                                                                                   >  1:616529/1‑150 (MQ=255)
                              aGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCgg                                                                                                                   >  2:836001/1‑150 (MQ=255)
                              aGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCgg                                                                                                                   <  1:2666938/150‑1 (MQ=255)
                              aGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCgg                                                                                                                   <  1:1716459/150‑1 (MQ=255)
                                   cTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACAt                                                                                                              <  1:1132256/150‑1 (MQ=255)
                                     gTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATAc                                                                                                            >  2:3255411/1‑150 (MQ=255)
                                     gTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATAc                                                                                                            >  2:174059/1‑150 (MQ=255)
                                      ttCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACt                                                                                                           >  1:1869535/1‑150 (MQ=255)
                                        ccATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAATGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTcc                                                                                                         >  2:1977019/1‑150 (MQ=255)
                                        ccATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTcc                                                                                                         >  1:1665721/1‑150 (MQ=255)
                                                tCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAgg                                                                                                 <  1:519545/150‑1 (MQ=255)
                                                tCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAgg                                                                                                 <  1:534408/150‑1 (MQ=255)
                                                   gcgTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGAtt                                                                                              <  1:2618193/150‑1 (MQ=255)
                                                    cgTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTc                                                                                             >  1:4271337/1‑150 (MQ=255)
                                                     gTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCt                                                                                            <  2:1634625/150‑1 (MQ=255)
                                                     gTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCt                                                                                            <  2:1029489/150‑1 (MQ=255)
                                                        gAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAAc                                                                                         <  1:4110382/150‑1 (MQ=255)
                                                         aaGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACg                                                                                        <  1:1394752/150‑1 (MQ=255)
                                                           ggCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGAc                                                                                      >  1:1324103/1‑150 (MQ=255)
                                                            gCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACa                                                                                     <  1:1017078/150‑1 (MQ=255)
                                                             cATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACaa                                                                                    <  1:3749295/150‑1 (MQ=255)
                                                                 aaaaCCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACAt                                                                                                              >  2:3174757/1‑120 (MQ=255)
                                                                 aaaaCCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTc                                                                                <  1:3864043/150‑1 (MQ=255)
                                                                  aaaCCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTcc                                                                               >  2:4233907/1‑150 (MQ=255)
                                                                   aaCCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCt                                                                              >  1:2035911/1‑150 (MQ=255)
                                                                    aCCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTc                                                                             >  2:3858009/1‑150 (MQ=255)
                                                                     ccTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCg                                                                            >  2:3489684/1‑150 (MQ=255)
                                                                          gaagaaCTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAAc                                                                       >  2:3920004/1‑150 (MQ=255)
                                                                          gaagaaCTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAAc                                                                       >  2:2716852/1‑150 (MQ=255)
                                                                           aagaaCTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACa                                                                      >  2:1771689/1‑150 (MQ=255)
                                                                           aagaaCTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACa                                                                      >  2:1515516/1‑150 (MQ=255)
                                                                              aaCTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATcc                                                                   <  1:4276391/150‑1 (MQ=255)
                                                                              aaCTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATcc                                                                   <  1:2137344/150‑1 (MQ=255)
                                                                              aaCTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATcc                                                                   <  1:4051088/150‑1 (MQ=255)
                                                                                 tgctgGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGa                                                                >  1:315836/1‑150 (MQ=255)
                                                                                        gAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACgg                                                         >  2:1668996/1‑150 (MQ=255)
                                                                                         aaaCACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGc                                                        >  2:3974691/1‑150 (MQ=255)
                                                                                         aaaCACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGc                                                        >  2:2118129/1‑150 (MQ=255)
                                                                                         aaaCAAGGCAAAGGCTACGGTTGTGAAGTTTGTAAACAAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGATGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGc                                                        >  1:3684109/1‑150 (MQ=255)
                                                                                           acacGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCAc                                                      <  2:3574606/150‑1 (MQ=255)
                                                                                             acGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGTAACGAATACATTCTGAAGCCGGAAAATACTCCGCTGCTGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCt                                                    <  1:2192639/150‑1 (MQ=255)
                                                                                             acGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCt                                                    <  1:859446/150‑1 (MQ=255)
                                                                                              cGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTa                                                   >  2:2680399/1‑150 (MQ=255)
                                                                                                  aaaGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCg                                               >  2:2415777/1‑150 (MQ=255)
                                                                                                    aGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGt                                             <  2:67451/150‑1 (MQ=255)
                                                                                                      gCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGa                                           <  2:2275379/150‑1 (MQ=255)
                                                                                                      gCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGa                                           >  1:2035477/1‑150 (MQ=255)
                                                                                                        tACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATc                                         <  2:1052214/150‑1 (MQ=255)
                                                                                                         aCGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATcc                                        >  2:508794/1‑150 (MQ=255)
                                                                                                           ggTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCcg                                      <  1:2394211/150‑1 (MQ=255)
                                                                                                            gTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCcgc                                     >  2:1118789/1‑150 (MQ=255)
                                                                                                             ttGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCGGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCcgcg                                    <  1:1221119/150‑1 (MQ=255)
                                                                                                               gtgAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGtt                                  <  2:1834086/150‑1 (MQ=255)
                                                                                                                 gAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTtct                                >  1:734744/1‑150 (MQ=255)
                                                                                                                 gAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTtct                                >  1:2716073/1‑150 (MQ=255)
                                                                                                                  aaGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGTATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTtctc                               <  1:3308140/150‑1 (MQ=255)
                                                                                                                  aaGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTtctc                               <  1:110173/150‑1 (MQ=255)
                                                                                                                  aaGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTtctc                               >  2:2953037/1‑150 (MQ=255)
                                                                                                                   aGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTcc                              >  1:3415489/1‑150 (MQ=255)
                                                                                                                          aaaCCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGt                       >  1:580341/1‑150 (MQ=255)
                                                                                                                          aaaCCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGt                       <  1:43171/150‑1 (MQ=255)
                                                                                                                           aaccaaccGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTg                      <  1:1155903/150‑1 (MQ=255)
                                                                                                                             ccaaccGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGaa                    >  1:1169790/1‑150 (MQ=255)
                                                                                                                               aaccGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAAt                  >  1:317513/1‑150 (MQ=255)
                                                                                                                                 ccGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCa                <  1:4200980/150‑1 (MQ=255)
                                                                                                                                  cGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCAc               >  2:3779190/1‑150 (MQ=255)
                                                                                                                                  cGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCAc               >  2:2051976/1‑150 (MQ=255)
                                                                                                                                  cGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACAGACATAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCAc               >  2:691785/1‑150 (MQ=255)
                                                                                                                                         tCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGaa        >  2:3951822/1‑150 (MQ=255)
                                                                                                                                         acGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTACCGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGaa        <  2:2742861/149‑1 (MQ=255)
                                                                                                                                          cGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGAAg       <  1:1258217/150‑1 (MQ=255)
                                                                                                                                          cGCTGCGGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGAAg       <  2:509905/150‑1 (MQ=255)
                                                                                                                                            ctgctgCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGAAggg     <  2:4095129/150‑1 (MQ=255)
                                                                                                                                             tgctgcCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGAAGGGc    <  1:833094/150‑1 (MQ=255)
                                                                                                                                             tgctgGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGAAGGGc    >  2:3434556/1‑150 (MQ=255)
                                                                                                                                               ctgCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGAAGGGCTg  <  2:1434679/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
ACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGAAGGGCTG  >  CP009273/3488820‑3489112

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CAACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGAAGGGCTGAT  >  CP009273/3488818‑3489114
                                                                                                                                                    |                                                                                                                                                    
CAACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCC                                                                                                                                                     <  SRR19973079.4017285/150‑1 (MQ=60)
  ACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCT                                                                                                                                                   >  SRR19973079.4222513/1‑150 (MQ=60)
    CTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCC                                                                                                                                                 >  SRR19973079.3538752/1‑150 (MQ=60)
    CTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCC                                                                                                                                                 >  SRR19973079.1334074/1‑150 (MQ=60)
    CTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCC                                                                                                                                                 >  SRR19973079.3950747/1‑150 (MQ=60)
    CTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCC                                                                                                                                                 >  SRR19973079.3951302/1‑150 (MQ=60)
     TGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCT                                                                                                                                                >  SRR19973079.2316508/1‑150 (MQ=60)
     TGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCT                                                                                                                                                <  SRR19973079.1964536/150‑1 (MQ=60)
     TGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCT                                                                                                                                                <  SRR19973079.371109/150‑1 (MQ=60)
      GTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTG                                                                                                                                               <  SRR19973079.1794159/150‑1 (MQ=60)
      GTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTG                                                                                                                                               <  SRR19973079.3992899/150‑1 (MQ=60)
         CGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTG                                                                                                                                            <  SRR19973079.4246820/150‑1 (MQ=60)
          GAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGG                                                                                                                                           <  SRR19973079.1046084/150‑1 (MQ=60)
          GAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGG                                                                                                                                           <  SRR19973079.2232733/150‑1 (MQ=60)
          GAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGG                                                                                                                                           >  SRR19973079.222114/1‑150 (MQ=60)
          GAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGG                                                                                                                                           >  SRR19973079.3406532/1‑150 (MQ=60)
             CACTTTGCTTATTCGCGTCAGGAACTGGTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAAC                                                                                                                                        <  SRR19973079.2757703/150‑1 (MQ=60)
                TTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAA                                                                                                                                     >  SRR19973079.294517/1‑150 (MQ=60)
                 TTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAAT                                                                                                                                    <  SRR19973079.2190632/150‑1 (MQ=60)
                 TTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAAT                                                                                                                                    >  SRR19973079.1681621/1‑150 (MQ=60)
                      TATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTCCGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATT                                                                                                                               >  SRR19973079.1168778/1‑150 (MQ=60)
                      TATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATT                                                                                                                               >  SRR19973079.1168619/1‑150 (MQ=60)
                          CGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGA                                                                                                                           >  SRR19973079.1400587/1‑150 (MQ=60)
                             GTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGC                                                                                                                        >  SRR19973079.3656103/1‑150 (MQ=60)
                              TCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCC                                                                                                                       <  SRR19973079.3571822/150‑1 (MQ=60)
                              TCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCC                                                                                                                       <  SRR19973079.1685303/150‑1 (MQ=60)
                                AGGTACTGTTACTTTTGATCCGCGTTGAAGGCATTAAAAGCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGG                                                                                                                     <  SRR19973079.1725636/150‑1 (MQ=60)
                                AGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGG                                                                                                                     >  SRR19973079.620115/1‑150 (MQ=60)
                                AGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGG                                                                                                                     >  SRR19973079.3066977/1‑150 (MQ=60)
                                AGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGG                                                                                                                     >  SRR19973079.620085/1‑150 (MQ=60)
                                AGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGG                                                                                                                     <  SRR19973079.1725619/150‑1 (MQ=60)
                                AGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGG                                                                                                                     <  SRR19973079.2680764/150‑1 (MQ=60)
                                  GAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAA                                                                                                                   <  SRR19973079.482294/150‑1 (MQ=60)
                                     ctggagttcagacgtgtgctcttccgatcTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACAT                                                                                                                <  SRR19973079.3191008/121‑1 (MQ=60)
                                     ctggagttcagacgtgtgctcttccgatcTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACAT                                                                                                                <  SRR19973079.3192351/121‑1 (MQ=60)
                                     CTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACAT                                                                                                                <  SRR19973079.1138726/150‑1 (MQ=60)
                                        TTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACT                                                                                                             >  SRR19973079.1879459/1‑150 (MQ=60)
                                        TTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACT                                                                                                             <  SRR19973079.813365/150‑1 (MQ=60)
                                         TCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTC                                                                                                            <  SRR19973079.174268/150‑1 (MQ=60)
                                          CCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCC                                                                                                           >  SRR19973079.1674734/1‑150 (MQ=60)
                                             TTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCT                                                                                                        <  SRR19973079.3861706/150‑1 (MQ=60)
                                             TTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCT                                                                                                        <  SRR19973079.3863305/150‑1 (MQ=60)
                                                GATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCA                                                                                                     >  SRR19973079.3044895/1‑150 (MQ=60)
                                                 ATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAG                                                                                                    <  SRR19973079.1625678/150‑1 (MQ=60)
                                                  TCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGG                                                                                                   <  SRR19973079.522686/150‑1 (MQ=60)
                                                  TCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGG                                                                                                   <  SRR19973079.2706044/150‑1 (MQ=60)
                                                  TCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGG                                                                                                   <  SRR19973079.522683/150‑1 (MQ=60)
                                                  TCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGG                                                                                                   <  SRR19973079.537620/150‑1 (MQ=60)
                                                     GCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATT                                                                                                <  SRR19973079.2631698/150‑1 (MQ=60)
                                                      CGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTC                                                                                               >  SRR19973079.4292930/1‑150 (MQ=60)
                                                       GTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCT                                                                                              <  SRR19973079.2590636/150‑1 (MQ=60)
                                                          GAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAAC                                                                                           <  SRR19973079.4131227/150‑1 (MQ=60)
                                                          GAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAAC                                                                                           >  SRR19973079.933157/1‑150 (MQ=60)
                                                           AAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACG                                                                                          <  SRR19973079.1402279/150‑1 (MQ=60)
                                                           AAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACG                                                                                          <  SRR19973079.1402413/150‑1 (MQ=60)
                                                             GGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGACTACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGAC                                                                                        >  SRR19973079.1203511/1‑150 (MQ=60)
                                                             GGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGAC                                                                                        >  SRR19973079.1202419/1‑150 (MQ=60)
                                                             GGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGAC                                                                                        >  SRR19973079.1331563/1‑150 (MQ=60)
                                                              GCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACA                                                                                       <  SRR19973079.1022777/150‑1 (MQ=60)
                                                              GCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACA                                                                                       <  SRR19973079.4072187/150‑1 (MQ=60)
                                                               CATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAA                                                                                      <  SRR19973079.3768504/150‑1 (MQ=60)
                                                                 TTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACT                                                                                    >  SRR19973079.3696989/1‑150 (MQ=60)
                                                                 TTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACT                                                                                    >  SRR19973079.123718/1‑150 (MQ=60)
                                                                   AAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTC                                                                                  >  SRR19973079.59954/1‑150 (MQ=60)
                                                                   AAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTC                                                                                  <  SRR19973079.3883717/150‑1 (MQ=60)
                                                                     AACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCT                                                                                >  SRR19973079.2046614/1‑150 (MQ=60)
                                                                     AACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCT                                                                                >  SRR19973079.2046605/1‑150 (MQ=60)
                                                                     AACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCT                                                                                >  SRR19973079.542637/1‑150 (MQ=60)
                                                                     AACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCT                                                                                >  SRR19973079.542642/1‑150 (MQ=60)
                                                                      ACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTCACGACAACTTCCTC                                                                               >  SRR19973079.2657701/1‑150 (MQ=60)
                                                                          TCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTA                                                                           <  SRR19973079.1614063/150‑1 (MQ=60)
                                                                          TCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTA                                                                           <  SRR19973079.3931339/150‑1 (MQ=60)
                                                                                AACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCC                                                                     >  SRR19973079.1885027/1‑150 (MQ=60)
                                                                                AACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCC                                                                     <  SRR19973079.4297998/150‑1 (MQ=60)
                                                                                AACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCC                                                                     <  SRR19973079.4297994/150‑1 (MQ=60)
                                                                                AACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCC                                                                     <  SRR19973079.2148352/150‑1 (MQ=60)
                                                                                AACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCC                                                                     <  SRR19973079.4071587/150‑1 (MQ=60)
                                                                                AACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCC                                                                     <  SRR19973079.2148343/150‑1 (MQ=60)
                                                                                 ACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCA                                                                    <  SRR19973079.4360936/150‑1 (MQ=60)
                                                                                 ACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCA                                                                    <  SRR19973079.1111612/150‑1 (MQ=60)
                                                                                   TGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGA                                                                  >  SRR19973079.317650/1‑150 (MQ=60)
                                                                                       GGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGA                                                              >  SRR19973079.3362294/1‑150 (MQ=60)
                                                                                       GGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGA                                                              <  SRR19973079.782922/150‑1 (MQ=60)
                                                                                           AAACAAGGCAAAGGCTACGGTTGTGAAGTTTGTAAACAAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGATGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGC                                                          >  SRR19973079.3702902/1‑150 (MQ=60)
                                                                                            AACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCA                                                         <  SRR19973079.1984336/150‑1 (MQ=60)
                                                                                               ACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGTAACGAATACATTCTGAAGCCGGAAAATACTCCGCTGCTGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCT                                                      <  SRR19973079.2203908/150‑1 (MQ=60)
                                                                                               ACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCT                                                      <  SRR19973079.864409/150‑1 (MQ=60)
                                                                                               ACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCT                                                      <  SRR19973079.863050/150‑1 (MQ=60)
                                                                                                     AAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGG                                                <  SRR19973079.1710002/150‑1 (MQ=60)
                                                                                                      AGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGT                                               >  SRR19973079.129172/1‑150 (MQ=60)
                                                                                                       GGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTG                                              <  SRR19973079.2093766/150‑1 (MQ=60)
                                                                                                        GCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGA                                             >  SRR19973079.4141703/1‑150 (MQ=60)
                                                                                                        GCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGA                                             >  SRR19973079.1944782/1‑150 (MQ=60)
                                                                                                        GCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGA                                             >  SRR19973079.2046179/1‑150 (MQ=60)
                                                                                                            CGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCC                                         >  SRR19973079.1815730/1‑150 (MQ=60)
                                                                                                             GGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCG                                        <  SRR19973079.2406586/150‑1 (MQ=60)
                                                                                                               TTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCGGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCG                                      <  SRR19973079.1228129/150‑1 (MQ=60)
                                                                                                                TGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGT                                     <  SRR19973079.1171828/150‑1 (MQ=60)
                                                                                                                 GTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTT                                    >  SRR19973079.3425081/1‑150 (MQ=60)
                                                                                                                   GAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCT                                  >  SRR19973079.738631/1‑150 (MQ=60)
                                                                                                                   GAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCT                                  >  SRR19973079.2730126/1‑150 (MQ=60)
                                                                                                                    AAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGTATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTC                                 <  SRR19973079.3325026/150‑1 (MQ=60)
                                                                                                                    AAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTC                                 <  SRR19973079.111115/150‑1 (MQ=60)
                                                                                                                    AAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCGGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTC                                 <  SRR19973079.3324932/150‑1 (MQ=60)
                                                                                                                     AGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCC                                >  SRR19973079.3433124/1‑150 (MQ=60)
                                                                                                                            AAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGT                         <  SRR19973079.43459/150‑1 (MQ=60)
                                                                                                                            AAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGT                         >  SRR19973079.583760/1‑150 (MQ=60)
                                                                                                                            AAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGT                         <  SRR19973079.43630/150‑1 (MQ=60)
                                                                                                                             AACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACTAATACATTCTGAAGCCGGAACATACTCCGCGGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTG                        <  SRR19973079.1162249/150‑1 (MQ=60)
                                                                                                                             AACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTG                        <  SRR19973079.1162542/150‑1 (MQ=60)
                                                                                                                               CCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAA                      >  SRR19973079.1176526/1‑150 (MQ=60)
                                                                                                                                CAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAA                     >  SRR19973079.1154016/1‑150 (MQ=60)
                                                                                                                                CAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAA                     >  SRR19973079.2673643/1‑150 (MQ=60)
                                                                                                                                 AACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAAT                    >  SRR19973079.319335/1‑150 (MQ=60)
                                                                                                                                   CCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCA                  <  SRR19973079.4222266/150‑1 (MQ=60)
                                                                                                                                     GTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACC                >  SRR19973079.2391257/1‑150 (MQ=60)
                                                                                                                                            CGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGAAG         <  SRR19973079.1265424/150‑1 (MQ=60)
                                                                                                                                            CGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGAAG         <  SRR19973079.1267101/150‑1 (MQ=60)
                                                                                                                                               TGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGAAGGGC      <  SRR19973079.837900/150‑1 (MQ=60)
                                                                                                                                                   GCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGAAGGGCTGAT  <  SRR19973079.4295823/150‑1 (MQ=60)
                                                                                                                                                   GCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGAAGGGCTGAT  <  SRR19973079.4295820/150‑1 (MQ=60)
                                                                                                                                                   GCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGAAGGGCTGAT  >  SRR19973079.1422449/1‑150 (MQ=60)
                                                                                                                                                   GCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGAAGGGCTGAT  >  SRR19973079.1422422/1‑150 (MQ=60)
                                                                                                                                                   GCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACGTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGAAGGGCTGAT  <  SRR19973079.1441273/150‑1 (MQ=60)
                                                                                                                                                   GCCCTCCTGCTGGAACGAATACAGTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACGTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGAAGGGCTGAT  <  SRR19973079.3851137/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                    
CAACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGAAGGGCTGAT  >  CP009273/3488818‑3489114

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑