Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,488,966 G→C 100% A533P (GCC→CCC)  nirB → nitrite reductase, large subunit, NAD(P)H‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,488,9660GC99.4% 669.4 / ‑3.8 167A533P (GCC→CCC) nirBnitrite reductase, large subunit, NAD(P)H‑binding
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base C (93/73);  total (93/74)
Fisher's exact test for biased strand distribution p-value = 4.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 169 total aligned reads displayed.

CCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGAAGGGC  >  CP009273/3488821‑3489110
                                                                                                                                                 |                                                                                                                                                
ccTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGccc                                                                                                                                                >  1:1213067/1‑148 (MQ=255)
 cTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCcct                                                                                                                                               >  1:3551589/1‑148 (MQ=255)
   gtgCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCcctcc                                                                                                                                             <  2:2120296/148‑1 (MQ=255)
   gtgCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCcctcc                                                                                                                                             <  2:2120412/148‑1 (MQ=255)
        aaCACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCctgctg                                                                                                                                        <  1:591140/148‑1 (MQ=255)
           acTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGaa                                                                                                                                     <  2:2939119/148‑1 (MQ=255)
              ttGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGa                                                                                                                                  >  1:965003/1‑148 (MQ=255)
              ttGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGa                                                                                                                                  >  1:2720034/1‑148 (MQ=255)
               tGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGaa                                                                                                                                 >  1:2311474/1‑148 (MQ=255)
               tGCTAATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACAGTCGGTTCTCTGCTGCCCTCCTGCTGGAACGaa                                                                                                                                 >  1:2311461/1‑148 (MQ=255)
                  ttATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATAc                                                                                                                              >  2:942783/1‑148 (MQ=255)
                  ttATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATAc                                                                                                                              >  1:160188/1‑148 (MQ=255)
                   tATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACa                                                                                                                             <  1:3148485/148‑1 (MQ=255)
                   tATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACa                                                                                                                             >  2:141867/1‑148 (MQ=255)
                    aTTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACAt                                                                                                                            >  1:1681788/1‑148 (MQ=255)
                     ttCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACAtt                                                                                                                           >  2:138297/1‑148 (MQ=255)
                      tCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTc                                                                                                                          >  2:1717168/1‑148 (MQ=255)
                         cgTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGa                                                                                                                       >  1:1632968/1‑148 (MQ=255)
                            cAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGc                                                                                                                    <  1:2431400/148‑1 (MQ=255)
                              ggAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACAAGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCg                                                                                                                  >  1:2246269/1‑148 (MQ=255)
                                 aCTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGaa                                                                                                               >  1:1426800/1‑148 (MQ=255)
                                  cTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAAc                                                                                                              <  2:2686965/148‑1 (MQ=255)
                                  cTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAAc                                                                                                              >  1:128865/1‑148 (MQ=255)
                                     ttCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATa                                                                                                           >  2:1074211/1‑148 (MQ=255)
                                      tCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATAc                                                                                                          >  1:2485297/1‑148 (MQ=255)
                                      tCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATAc                                                                                                          <  1:1043389/148‑1 (MQ=255)
                                      tCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATAc                                                                                                          <  1:7995/148‑1 (MQ=255)
                                       ccATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACt                                                                                                         >  1:3004580/1‑148 (MQ=255)
                                          tttGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCg                                                                                                      <  1:1026526/148‑1 (MQ=255)
                                           ttGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGACCATACTCCGc                                                                                                     >  2:2165871/1‑148 (MQ=255)
                                           ttGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGc                                                                                                     >  2:1874707/1‑148 (MQ=255)
                                           ttGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGc                                                                                                     >  2:2165848/1‑148 (MQ=255)
                                             gATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTg                                                                                                   <  2:566385/148‑1 (MQ=255)
                                               tCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCa                                                                                                 <  2:3473014/148‑1 (MQ=255)
                                                ccGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAg                                                                                                >  1:982790/1‑148 (MQ=255)
                                                 cgcgTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAgg                                                                                               <  1:1002561/148‑1 (MQ=255)
                                                  gcgTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGa                                                                                              <  2:2033479/148‑1 (MQ=255)
                                                  gcgTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGa                                                                                              <  2:128865/148‑1 (MQ=255)
                                                     ttGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTc                                                                                           <  1:2998190/148‑1 (MQ=255)
                                                      tGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCt                                                                                          >  1:2968695/1‑148 (MQ=255)
                                                         aGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAAc                                                                                       >  1:2136247/1‑148 (MQ=255)
                                                           gCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGa                                                                                     >  1:640569/1‑148 (MQ=255)
                                                           gCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGa                                                                                     >  1:736163/1‑148 (MQ=255)
                                                           gCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGa                                                                                     >  1:2429985/1‑148 (MQ=255)
                                                             aTTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACa                                                                                   <  2:2311474/148‑1 (MQ=255)
                                                              ttAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACaa                                                                                  >  2:1688794/1‑148 (MQ=255)
                                                                  aaCCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTc                                                                              <  2:851872/148‑1 (MQ=255)
                                                                  aaCCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTc                                                                              <  2:2246269/148‑1 (MQ=255)
                                                                  aaCCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTc                                                                              <  2:2183047/148‑1 (MQ=255)
                                                                   aCCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTcc                                                                             >  2:246513/1‑148 (MQ=255)
                                                                   aCCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTcc                                                                             <  2:2312461/148‑1 (MQ=255)
                                                                   aCCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTcc                                                                             <  2:2836119/148‑1 (MQ=255)
                                                                     cTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTc                                                                           >  1:3210734/1‑148 (MQ=255)
                                                                      ttCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCg                                                                          <  2:1830890/148‑1 (MQ=255)
                                                                         gaagaaCTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTa                                                                       <  2:2618339/148‑1 (MQ=255)
                                                                         gaagaaCTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTa                                                                       <  2:811157/148‑1 (MQ=255)
                                                                         gaagaaCTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTa                                                                       >  2:108857/1‑148 (MQ=255)
                                                                         gaagaaCTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTa                                                                       <  2:2618346/148‑1 (MQ=255)
                                                                         gaagaaCTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTa                                                                       >  2:108882/1‑148 (MQ=255)
                                                                          aagaaCTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTaa                                                                      <  2:1681788/148‑1 (MQ=255)
                                                                           agaaCTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAAc                                                                     >  2:448333/1‑148 (MQ=255)
                                                                           agaaCTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAAc                                                                     >  2:449077/1‑148 (MQ=255)
                                                                               ctgctgGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATcc                                                                 <  1:2598746/148‑1 (MQ=255)
                                                                                 gctgGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAg                                                               >  2:1506193/1‑148 (MQ=255)
                                                                                    ggCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGaaa                                                            >  1:2638359/1‑148 (MQ=255)
                                                                                     gCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAg                                                           <  2:1387786/148‑1 (MQ=255)
                                                                                      cGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGa                                                          <  1:2356837/148‑1 (MQ=255)
                                                                                         aaCACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACgg                                                       <  2:3417240/148‑1 (MQ=255)
                                                                                         aaCACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACgg                                                       <  1:526793/148‑1 (MQ=255)
                                                                                          acacGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGc                                                      >  2:3181621/1‑148 (MQ=255)
                                                                                          acacGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGc                                                      >  2:785395/1‑148 (MQ=255)
                                                                                           cacGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCa                                                     >  2:574201/1‑148 (MQ=255)
                                                                                               gCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTa                                                 <  2:1840579/148‑1 (MQ=255)
                                                                                               gCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTa                                                 <  2:154018/148‑1 (MQ=255)
                                                                                                 aaaGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACt                                               <  2:3210734/148‑1 (MQ=255)
                                                                                                  aaGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTc                                              <  1:2034577/148‑1 (MQ=255)
                                                                                                      cTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTg                                          <  2:2270433/148‑1 (MQ=255)
                                                                                                         cGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATc                                       <  1:1008519/148‑1 (MQ=255)
                                                                                                           gTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATccc                                     >  1:266528/1‑148 (MQ=255)
                                                                                                             tgtgAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCcgc                                   >  1:2853187/1‑148 (MQ=255)
                                                                                                              gtgAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCcgcg                                  >  1:2186622/1‑148 (MQ=255)
                                                                                                                          aaccaaccGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCgg                      >  1:1920688/1‑148 (MQ=255)
                                                                                                                          aaccaaccGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCgg                      <  2:2048610/148‑1 (MQ=255)
                                                                                                                          aaccaaccGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCgg                      >  1:309488/1‑148 (MQ=255)
                                                                                                                              aaccGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGaa                  <  1:138297/148‑1 (MQ=255)
                                                                                                                                ccGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAAt                >  1:3182635/1‑148 (MQ=255)
                                                                                                                                 cGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATc               >  2:1919423/1‑148 (MQ=255)
                                                                                                                                 cGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATc               >  1:1627629/1‑148 (MQ=255)
                                                                                                                                  gTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCa              <  2:394551/148‑1 (MQ=255)
                                                                                                                                  gTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCa              >  2:482540/1‑148 (MQ=255)
                                                                                                                                    cGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCAcc            <  2:3551589/148‑1 (MQ=255)
                                                                                                                                     ggTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCAccc           <  2:937489/148‑1 (MQ=255)
                                                                                                                                       ttCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCg         >  1:368389/1‑148 (MQ=255)
                                                                                                                                       ttCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCg         >  2:284435/1‑148 (MQ=255)
                                                                                                                                            tgctgcCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGAAgg    <  2:1649156/148‑1 (MQ=255)
                                                                                                                                             gctgCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGAAggg   <  2:3292307/148‑1 (MQ=255)
                                                                                                                                             gctgCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGAAggg   <  1:171769/148‑1 (MQ=255)
                                                                                                                                             gctgCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGAAggg   <  1:171821/148‑1 (MQ=255)
                                                                                                                                              ctgCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGAAGGGc  <  2:1714527/148‑1 (MQ=255)
                                                                                                                                              ctgCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGAAGGGc  >  1:2863339/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGAAGGGC  >  CP009273/3488821‑3489110

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACAACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGAAGGGCTGATG  >  CP009273/3488817‑3489115
                                                                                                                                                     |                                                                                                                                                     
ACAACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGC                                                                                                                                                       >  SRR19973066.2062850/1‑150 (MQ=60)
  AACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCC                                                                                                                                                     >  SRR19973066.1221587/1‑150 (MQ=60)
   ACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCT                                                                                                                                                    >  SRR19973066.3575157/1‑150 (MQ=60)
   ACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCT                                                                                                                                                    <  SRR19973066.1264827/150‑1 (MQ=60)
   ACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCT                                                                                                                                                    <  SRR19973066.1264837/150‑1 (MQ=60)
            AACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGA                                                                                                                                           >  SRR19973066.525042/1‑150 (MQ=60)
            AACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGA                                                                                                                                           <  SRR19973066.595247/150‑1 (MQ=60)
               ACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACG                                                                                                                                        <  SRR19973066.2240763/150‑1 (MQ=60)
                CTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGA                                                                                                                                       >  SRR19973066.972093/1‑150 (MQ=60)
                CTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGA                                                                                                                                       >  SRR19973066.2738327/1‑150 (MQ=60)
                 TTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAA                                                                                                                                      >  SRR19973066.2327289/1‑150 (MQ=60)
                 TTTGCTAATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACAGTCGGTTCTCTGCTGCCCTCCTGCTGGAACGAA                                                                                                                                      >  SRR19973066.2327276/1‑150 (MQ=60)
                    GCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATAC                                                                                                                                   >  SRR19973066.161654/1‑150 (MQ=60)
                     CTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACA                                                                                                                                  >  SRR19973066.3353674/1‑150 (MQ=60)
                      TTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTATAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACAT                                                                                                                                 >  SRR19973066.1694094/1‑150 (MQ=60)
                      TTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACAT                                                                                                                                 >  SRR19973066.1694033/1‑150 (MQ=60)
                       TATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATT                                                                                                                                <  SRR19973066.3169481/150‑1 (MQ=60)
                        ccccgccacaccacccgccccaacagccccgcctgGAAGGCATCAAAACCTTCGAACAACCGCTCGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTC                                                                                                                               <  SRR19973066.3311081/115‑1 (MQ=60)
                           CGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGA                                                                                                                            >  SRR19973066.1644839/1‑150 (MQ=60)
                              GTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGC                                                                                                                         >  SRR19973066.2816851/1‑150 (MQ=60)
                                CAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCG                                                                                                                       <  SRR19973066.2447949/150‑1 (MQ=60)
                                CAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACAAGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTAGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCG                                                                                                                       >  SRR19973066.2261754/1‑150 (MQ=60)
                                   GAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAA                                                                                                                    >  SRR19973066.1436992/1‑150 (MQ=60)
                                    AACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAAC                                                                                                                   >  SRR19973066.130136/1‑150 (MQ=60)
                                    AACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAAC                                                                                                                   >  SRR19973066.2047667/1‑150 (MQ=60)
                                     ACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACA                                                                                                                  <  SRR19973066.578059/150‑1 (MQ=60)
                                        GTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATAC                                                                                                               >  SRR19973066.2502252/1‑150 (MQ=60)
                                         TTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACT                                                                                                              <  SRR19973066.1309567/150‑1 (MQ=60)
                                         TTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACT                                                                                                              >  SRR19973066.3024562/1‑150 (MQ=60)
                                          TCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTC                                                                                                             <  SRR19973066.8130/150‑1 (MQ=60)
                                          TCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTC                                                                                                             <  SRR19973066.1051060/150‑1 (MQ=60)
                                          TCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTC                                                                                                             <  SRR19973066.1051014/150‑1 (MQ=60)
                                              TTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCT                                                                                                         <  SRR19973066.1033467/150‑1 (MQ=60)
                                              TTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCT                                                                                                         <  SRR19973066.1034082/150‑1 (MQ=60)
                                                  ATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAG                                                                                                     >  SRR19973066.990042/1‑150 (MQ=60)
                                                   TCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGG                                                                                                    <  SRR19973066.2344146/150‑1 (MQ=60)
                                                     CGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGAT                                                                                                  <  SRR19973066.1009950/150‑1 (MQ=60)
                                                     CGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGAT                                                                                                  >  SRR19973066.787071/1‑150 (MQ=60)
                                                        GTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCT                                                                                               >  SRR19973066.2988326/1‑150 (MQ=60)
                                                        GTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCT                                                                                               >  SRR19973066.2988324/1‑150 (MQ=60)
                                                         TTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTA                                                                                              <  SRR19973066.3018088/150‑1 (MQ=60)
                                                           GAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAAC                                                                                            >  SRR19973066.2151068/1‑150 (MQ=60)
                                                             AGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGA                                                                                          >  SRR19973066.645198/1‑150 (MQ=60)
                                                             AGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGA                                                                                          >  SRR19973066.741628/1‑150 (MQ=60)
                                                             AGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGA                                                                                          >  SRR19973066.2446515/1‑150 (MQ=60)
                                                             AGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGA                                                                                          <  SRR19973066.27467/150‑1 (MQ=60)
                                                                   TAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTT                                                                                    >  SRR19973066.944309/1‑150 (MQ=60)
                                                                       ACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTC                                                                                >  SRR19973066.3232015/1‑150 (MQ=60)
                                                                       ACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTC                                                                                <  SRR19973066.2267843/150‑1 (MQ=60)
                                                                           TCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTA                                                                            <  SRR19973066.2466719/150‑1 (MQ=60)
                                                                                 AACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCC                                                                      >  SRR19973066.155448/1‑150 (MQ=60)
                                                                                 AACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCC                                                                      >  SRR19973066.1853732/1‑150 (MQ=60)
                                                                                   CTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAG                                                                    <  SRR19973066.2616336/150‑1 (MQ=60)
                                                                                    TGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGA                                                                   >  SRR19973066.1660656/1‑150 (MQ=60)
                                                                                      CTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAA                                                                 >  SRR19973066.2656115/1‑150 (MQ=60)
                                                                                      CTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAA                                                                 >  SRR19973066.2656114/1‑150 (MQ=60)
                                                                                       TGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAG                                                                >  SRR19973066.3070109/1‑150 (MQ=60)
                                                                                          CGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACG                                                             <  SRR19973066.2372829/150‑1 (MQ=60)
                                                                                             AACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCA                                                          <  SRR19973066.530652/150‑1 (MQ=60)
                                                                                                     AAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCG                                                  >  SRR19973066.2892261/1‑150 (MQ=60)
                                                                                                      AAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGG                                                 <  SRR19973066.2048772/150‑1 (MQ=60)
                                                                                                             CGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCC                                          <  SRR19973066.1015945/150‑1 (MQ=60)
                                                                                                             CGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCC                                          >  SRR19973066.268517/1‑150 (MQ=60)
                                                                                                               GTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGC                                        >  SRR19973066.2094982/1‑150 (MQ=60)
                                                                                                               GTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGC                                        >  SRR19973066.2872363/1‑150 (MQ=60)
                                                                                                                TTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCG                                       >  SRR19973066.2201794/1‑150 (MQ=60)
                                                                                                                    GAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCT                                   >  SRR19973066.2414837/1‑150 (MQ=60)
                                                                                                                    GAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCT                                   >  SRR19973066.679890/1‑150 (MQ=60)
                                                                                                                            TAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGG                           >  SRR19973066.311750/1‑150 (MQ=60)
                                                                                                                            TAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGG                           >  SRR19973066.1934348/1‑150 (MQ=60)
                                                                                                                            TAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGG                           >  SRR19973066.311747/1‑150 (MQ=60)
                                                                                                                             AAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGT                          >  SRR19973066.1275032/1‑150 (MQ=60)
                                                                                                                               ACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGA                        >  SRR19973066.25857/1‑150 (MQ=60)
                                                                                                                                  AACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAAT                     >  SRR19973066.3203808/1‑150 (MQ=60)
                                                                                                                                  AACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAAT                     <  SRR19973066.139630/150‑1 (MQ=60)
                                                                                                                                  AACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAAT                     >  SRR19973066.3203821/1‑150 (MQ=60)
                                                                                                                                   ACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATC                    >  SRR19973066.1639436/1‑150 (MQ=60)
                                                                                                                                     CGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTAATCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCAC                  >  SRR19973066.1620097/1‑150 (MQ=60)
                                                                                                                                         GGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCG              >  SRR19973066.371278/1‑150 (MQ=60)
                                                                                                                                                TGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGAAGGGC       >  SRR19973066.2882561/1‑150 (MQ=60)
                                                                                                                                                 GCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGAAGGGCT      <  SRR19973066.173354/150‑1 (MQ=60)
                                                                                                                                                 GCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGAAGGGCT      <  SRR19973066.173301/150‑1 (MQ=60)
                                                                                                                                                   TGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGAAGGGCTGA    >  SRR19973066.716527/1‑150 (MQ=60)
                                                                                                                                                   TGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGAAGGGCTGA    >  SRR19973066.716564/1‑150 (MQ=60)
                                                                                                                                                     CCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGAAGGGCTGATG  >  SRR19973066.3478995/1‑150 (MQ=60)
                                                                                                                                                     CCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGAAGGGCTGATG  >  SRR19973066.3478991/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
ACAACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGAAGGGCTGATG  >  CP009273/3488817‑3489115

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑