Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F247 I0 R1 1496 299.8 9582018 97.8% 9371213 149.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,488,966 G→C 100% A533P (GCC→CCC)  nirB → nitrite reductase, large subunit, NAD(P)H‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,488,9660GC98.4% 1008.9 / 5.1 249A533P (GCC→CCC) nirBnitrite reductase, large subunit, NAD(P)H‑binding
Reads supporting (aligned to +/- strand):  ref base G (0/4);  new base C (136/109);  total (136/113)
Fisher's exact test for biased strand distribution p-value = 4.12e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 249 total aligned reads displayed.

CCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGAAGG  >  CP009273/3488821‑3489108
                                                                                                                                                 |                                                                                                                                              
  tgtgCGAACACTTTGCTTATTCGCGTCAGGAACTGGTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCcctcc                                                                                                                                           <  2:2475572/149‑1 (MQ=255)
   gtgCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCcctcct                                                                                                                                          <  1:78058/149‑1 (MQ=255)
     gCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCctgc                                                                                                                                        <  2:64978/149‑1 (MQ=255)
     gCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCctgc                                                                                                                                        <  2:479570/149‑1 (MQ=255)
       gAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCctgctg                                                                                                                                      >  2:1141765/1‑149 (MQ=255)
       gAACACGTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCctgctg                                                                                                                                      >  2:3528927/1‑149 (MQ=255)
          cacTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGaa                                                                                                                                   <  2:3703540/149‑1 (MQ=255)
            cTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACg                                                                                                                                 >  1:3210596/1‑149 (MQ=255)
            cTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACg                                                                                                                                 >  1:1747964/1‑149 (MQ=255)
               tGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAAt                                                                                                                              <  2:3689955/149‑1 (MQ=255)
                 cTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATAc                                                                                                                            <  1:3711914/149‑1 (MQ=255)
                   tATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACAt                                                                                                                          >  2:1949951/1‑149 (MQ=255)
                     ttCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTc                                                                                                                        <  2:3470989/149‑1 (MQ=255)
                        gcgTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGa                                                                                                                     <  2:2175523/149‑1 (MQ=255)
                           tCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGc                                                                                                                  <  2:1582707/149‑1 (MQ=255)
                           tCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGc                                                                                                                  <  2:2847012/149‑1 (MQ=255)
                            cAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGcc                                                                                                                 <  2:4308575/149‑1 (MQ=255)
                            cAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGcc                                                                                                                 >  2:829673/1‑149 (MQ=255)
                              ggAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACAGTCTGAAGCCgg                                                                                                               >  2:727818/1‑149 (MQ=255)
                                aaCTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGaa                                                                                                             >  2:1235904/1‑149 (MQ=255)
                                 aCTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAAc                                                                                                            <  2:2594331/149‑1 (MQ=255)
                                 aCTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAAc                                                                                                            <  2:776427/149‑1 (MQ=255)
                                 aCTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAAc                                                                                                            >  1:388087/1‑149 (MQ=255)
                                   tGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACAt                                                                                                          <  1:528018/149‑1 (MQ=255)
                                   tGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACAt                                                                                                          >  2:2136606/1‑149 (MQ=255)
                                    gTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATa                                                                                                         >  1:3048880/1‑149 (MQ=255)
                                      tCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACt                                                                                                       >  1:3087928/1‑149 (MQ=255)
                                      tCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACt                                                                                                       <  1:4541746/149‑1 (MQ=255)
                                       ccATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTc                                                                                                      >  2:2024645/1‑149 (MQ=255)
                                          tttGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGc                                                                                                   >  2:1064614/1‑149 (MQ=255)
                                             gATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGc                                                                                                >  2:3004312/1‑149 (MQ=255)
                                             gATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGc                                                                                                >  2:3618954/1‑149 (MQ=255)
                                                ccGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAgg                                                                                             <  1:3821788/149‑1 (MQ=255)
                                                  gcgTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGAt                                                                                           <  2:3910677/149‑1 (MQ=255)
                                                     ttGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCt                                                                                        >  2:1038826/1‑149 (MQ=255)
                                                     ttGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCt                                                                                        <  2:4651984/149‑1 (MQ=255)
                                                      tGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTa                                                                                       <  1:3576425/149‑1 (MQ=255)
                                                      tGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTa                                                                                       >  1:4682633/1‑149 (MQ=255)
                                                      tGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTa                                                                                       <  1:3576404/149‑1 (MQ=255)
                                                       gAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTaa                                                                                      <  1:1154907/149‑1 (MQ=255)
                                                       gAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTaa                                                                                      <  1:4767240/149‑1 (MQ=255)
                                                         aGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACg                                                                                    >  1:2864540/1‑149 (MQ=255)
                                                         aGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAACTTAGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCTGAACATACACCGCTGCAGGATTCTAACg                                                                                    >  2:51517/1‑149 (MQ=255)
                                                            cATTAAAACCTTCGAAGAACTGCTGGCTAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACa                                                                                 >  2:1794223/1‑149 (MQ=255)
                                                            cATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACa                                                                                 >  2:1094140/1‑149 (MQ=255)
                                                               tAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACt                                                                              >  1:4636732/1‑149 (MQ=255)
                                                                aaaaCCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACtt                                                                             >  2:2735775/1‑149 (MQ=255)
                                                                 aaaCCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTAGGGTTGTTTAGTTTGTAAACAAACCGTGGGTTCGCTGCTGCCCTCCTGCTGGAACTAATAAATTCTGAAGCCGTAACATACTCCGCTGCAGGATTATAACGACAACTTc                                                                            <  1:1613634/149‑1 (MQ=255)
                                                                 aaaCCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTc                                                                            >  1:1483198/1‑149 (MQ=255)
                                                                  aaCCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTcc                                                                           >  1:1948024/1‑149 (MQ=255)
                                                                   aCCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCt                                                                          >  1:90380/1‑149 (MQ=255)
                                                                   aCCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCt                                                                          <  2:4742728/149‑1 (MQ=255)
                                                                   aCCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCt                                                                          >  2:3903663/1‑149 (MQ=255)
                                                                    ccTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTc                                                                         <  1:2729008/149‑1 (MQ=255)
                                                                      ttCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGc                                                                       >  2:2335371/1‑149 (MQ=255)
                                                                         gaagaaCTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGACCATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTaa                                                                    >  2:2989626/1‑149 (MQ=255)
                                                                         gaagaaCTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTaa                                                                    >  2:1149594/1‑149 (MQ=255)
                                                                          aagaaCTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAAc                                                                   <  2:2668276/149‑1 (MQ=255)
                                                                          aagaaCTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAAc                                                                   >  1:2962993/1‑149 (MQ=255)
                                                                           agaaCTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACa                                                                  >  2:3651809/1‑149 (MQ=255)
                                                                           agaaCTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACa                                                                  >  1:2645948/1‑149 (MQ=255)
                                                                           agaaCTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACa                                                                  <  1:4298138/149‑1 (MQ=255)
                                                                            gaaCTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACAt                                                                 <  1:954553/149‑1 (MQ=255)
                                                                            gaaCTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACAt                                                                 >  1:3350979/1‑149 (MQ=255)
                                                                             aaCTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATc                                                                <  1:4062484/149‑1 (MQ=255)
                                                                              aCTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATcc                                                               <  2:4096265/149‑1 (MQ=255)
                                                                              aCTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCGGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATcc                                                               <  1:2216919/149‑1 (MQ=255)
                                                                               ctgctgGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCa                                                              >  1:1729792/1‑149 (MQ=255)
                                                                               ctgctgGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCa                                                              >  2:2652487/1‑149 (MQ=255)
                                                                                tgctgGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTCACATCCAg                                                             >  2:400673/1‑149 (MQ=255)
                                                                                   tgGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGaaa                                                          >  1:3119220/1‑149 (MQ=255)
                                                                                   tgGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGaaa                                                          <  2:613279/149‑1 (MQ=255)
                                                                                   tgGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGaaa                                                          >  2:138683/1‑149 (MQ=255)
                                                                                    ggCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAg                                                         >  1:3919939/1‑149 (MQ=255)
                                                                                     gCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGa                                                        <  1:2088188/149‑1 (MQ=255)
                                                                                      cGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGAc                                                       <  2:1452562/149‑1 (MQ=255)
                                                                                       gAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACg                                                      >  2:111210/1‑149 (MQ=255)
                                                                                               gCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTAc                                              >  1:1602896/1‑149 (MQ=255)
                                                                                               gCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTAc                                              >  1:2013867/1‑149 (MQ=255)
                                                                                               gCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTAc                                              <  1:1101749/149‑1 (MQ=255)
                                                                                               gCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTAc                                              >  2:2423667/1‑149 (MQ=255)
                                                                                               gCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTAc                                              >  2:2314401/1‑149 (MQ=255)
                                                                                                cAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACt                                             <  2:186204/149‑1 (MQ=255)
                                                                                                  aaGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCg                                           >  2:817587/1‑149 (MQ=255)
                                                                                                   aGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCgg                                          >  1:2431120/1‑149 (MQ=255)
                                                                                                      cTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGa                                       <  2:186847/149‑1 (MQ=255)
                                                                                                       tACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGAt                                      <  1:4139087/149‑1 (MQ=255)
                                                                                                         cGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATcc                                    <  2:2398474/149‑1 (MQ=255)
                                                                                                               tgAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGtt                              >  1:799847/1‑149 (MQ=255)
                                                                                                                gAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTtc                             <  2:3231962/149‑1 (MQ=255)
                                                                                                                   gTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACACCTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTcc                          >  1:990615/1‑149 (MQ=255)
                                                                                                                        tAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCg                     <  1:4439454/149‑1 (MQ=255)
                                                                                                                         aaaCCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCgg                    >  1:3430424/1‑149 (MQ=255)
                                                                                                                          aaccaaccGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGt                   >  1:758973/1‑149 (MQ=255)
                                                                                                                          aaccaaccGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGt                   >  1:2106369/1‑149 (MQ=255)
                                                                                                                              aaccGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGaaa               <  2:1348467/149‑1 (MQ=255)
                                                                                                                                ccGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATc             >  2:1760904/1‑149 (MQ=255)
                                                                                                                                ccGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATc             >  2:3283356/1‑149 (MQ=255)
                                                                                                                                   tCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCAcc          <  1:660386/149‑1 (MQ=255)
                                                                                                                                         cGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGaa    <  2:4437349/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                              
CCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGAAGG  >  CP009273/3488821‑3489108

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CAACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGAAGGGCTGATG  >  CP009273/3488818‑3489115
                                                                                                                                                    |                                                                                                                                                     
CAACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCC                                                                                                                                                      >  SRR19973071.4718140/1‑150 (MQ=60)
  ACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCT                                                                                                                                                    >  SRR19973071.4748271/1‑150 (MQ=60)
    CTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGTGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCC                                                                                                                                                  <  SRR19973071.4375792/150‑1 (MQ=60)
      GTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTG                                                                                                                                                >  SRR19973071.3219701/1‑150 (MQ=60)
      GTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTG                                                                                                                                                <  SRR19973071.78461/150‑1 (MQ=60)
      GTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTG                                                                                                                                                <  SRR19973071.78094/150‑1 (MQ=60)
         CGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTG                                                                                                                                             <  SRR19973071.4532821/150‑1 (MQ=60)
          GAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGG                                                                                                                                            >  SRR19973071.795393/1‑150 (MQ=60)
             CACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAAC                                                                                                                                         <  SRR19973071.4685316/150‑1 (MQ=60)
              ACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACG                                                                                                                                        >  SRR19973071.1754496/1‑150 (MQ=60)
              ACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACG                                                                                                                                        <  SRR19973071.2395190/150‑1 (MQ=60)
              ACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACG                                                                                                                                        >  SRR19973071.3221839/1‑150 (MQ=60)
               CTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGA                                                                                                                                       >  SRR19973071.3567400/1‑150 (MQ=60)
                 TTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAAT                                                                                                                                     >  SRR19973071.3674047/1‑150 (MQ=60)
                    CTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACA                                                                                                                                  <  SRR19973071.3318580/150‑1 (MQ=60)
                    CTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACA                                                                                                                                  <  SRR19973071.3725065/150‑1 (MQ=60)
                        TTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCT                                                                                                                              <  SRR19973071.502189/150‑1 (MQ=60)
                          CGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGA                                                                                                                            <  SRR19973071.3118240/150‑1 (MQ=60)
                             GTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGC                                                                                                                         >  SRR19973071.2092802/1‑150 (MQ=60)
                                 GGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGA                                                                                                                     >  SRR19973071.1756398/1‑150 (MQ=60)
                                   AACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAAC                                                                                                                   >  SRR19973071.389308/1‑150 (MQ=60)
                                   AACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAAC                                                                                                                   >  SRR19973071.389298/1‑150 (MQ=60)
                                     CTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACAT                                                                                                                 >  SRR19973071.1888557/1‑150 (MQ=60)
                                      TGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATA                                                                                                                >  SRR19973071.3059712/1‑150 (MQ=60)
                                      TGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATA                                                                                                                <  SRR19973071.530001/150‑1 (MQ=60)
                                        TTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCACAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGACCATACT                                                                                                              >  SRR19973071.3099271/1‑150 (MQ=60)
                                        TTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACT                                                                                                              >  SRR19973071.4301621/1‑150 (MQ=60)
                                        TTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACT                                                                                                              <  SRR19973071.426790/150‑1 (MQ=60)
                                        TTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACT                                                                                                              >  SRR19973071.3098839/1‑150 (MQ=60)
                                         TCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTC                                                                                                             <  SRR19973071.4557926/150‑1 (MQ=60)
                                          CCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCC                                                                                                            >  SRR19973071.283294/1‑150 (MQ=60)
                                          CCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACAAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCA                                                                                                            >  SRR19973071.283334/1‑150 (MQ=60)
                                              TTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTG                                                                                                        >  SRR19973071.3699536/1‑150 (MQ=60)
                                                   CCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGA                                                                                                   <  SRR19973071.3835346/150‑1 (MQ=60)
                                                       GTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCT                                                                                               >  SRR19973071.3430361/1‑150 (MQ=60)
                                                       GTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCT                                                                                               <  SRR19973071.656097/150‑1 (MQ=60)
                                                       GTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCT                                                                                               >  SRR19973071.1990046/1‑150 (MQ=60)
                                                       GTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCT                                                                                               >  SRR19973071.498184/1‑150 (MQ=60)
                                                        TTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTA                                                                                              <  SRR19973071.2178317/150‑1 (MQ=60)
                                                        TTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTA                                                                                              >  SRR19973071.4699287/1‑150 (MQ=60)
                                                        TTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTA                                                                                              >  SRR19973071.4699274/1‑150 (MQ=60)
                                                         TGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAA                                                                                             <  SRR19973071.3588839/150‑1 (MQ=60)
                                                         TGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAA                                                                                             <  SRR19973071.3588860/150‑1 (MQ=60)
                                                         TGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAA                                                                                             >  SRR19973071.3864399/1‑150 (MQ=60)
                                                          GAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAAC                                                                                            <  SRR19973071.4784082/150‑1 (MQ=60)
                                                          GAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAAC                                                                                            <  SRR19973071.1158918/150‑1 (MQ=60)
                                                           AAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACG                                                                                           >  SRR19973071.2874744/1‑150 (MQ=60)
                                                           AAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACG                                                                                           >  SRR19973071.2874926/1‑150 (MQ=60)
                                                                 TTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACT                                                                                     >  SRR19973071.4653249/1‑150 (MQ=60)
                                                                 TTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACT                                                                                     <  SRR19973071.948928/150‑1 (MQ=60)
                                                                   AAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTC                                                                                   >  SRR19973071.1488586/1‑150 (MQ=60)
                                                                    AAACCTTCGAAGAACTGCTGGCTAAACACGGCAAAGGCTAGGGTTGTTTAGTTTGTAAACAAACCGTGGGTTCGCTGCTGCCCTCCTGCTGGAACTAATAAATTCTGAAGCCGTAACATACTCCGCTGCAGGATTATAACGACAACTTCC                                                                                  <  SRR19973071.1619363/150‑1 (MQ=60)
                                                                    AAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCC                                                                                  >  SRR19973071.1955113/1‑150 (MQ=60)
                                                                     AACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCT                                                                                 >  SRR19973071.90851/1‑150 (MQ=60)
                                                                       CCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCG                                                                               <  SRR19973071.2738705/150‑1 (MQ=60)
                                                                            GAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAAC                                                                          >  SRR19973071.2066240/1‑150 (MQ=60)
                                                                            GAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAAC                                                                          >  SRR19973071.2973598/1‑150 (MQ=60)
                                                                             AAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACA                                                                         >  SRR19973071.2655460/1‑150 (MQ=60)
                                                                             AAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACA                                                                         >  SRR19973071.2655556/1‑150 (MQ=60)
                                                                             AAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAACTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACA                                                                         <  SRR19973071.3588848/150‑1 (MQ=60)
                                                                              AGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACAT                                                                        >  SRR19973071.3362869/1‑150 (MQ=60)
                                                                              AGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACAT                                                                        <  SRR19973071.4313350/150‑1 (MQ=60)
                                                                               GAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATC                                                                       <  SRR19973071.958022/150‑1 (MQ=60)
                                                                                AACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCC                                                                      <  SRR19973071.4077042/150‑1 (MQ=60)
                                                                                AACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCC                                                                      <  SRR19973071.4076855/150‑1 (MQ=60)
                                                                                 ACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCA                                                                     <  SRR19973071.3470435/150‑1 (MQ=60)
                                                                                 ACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCA                                                                     >  SRR19973071.1736234/1‑150 (MQ=60)
                                                                                 ACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCGGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCA                                                                     <  SRR19973071.2225110/150‑1 (MQ=60)
                                                                                  CTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAG                                                                    <  SRR19973071.1660910/150‑1 (MQ=60)
                                                                                     CTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAA                                                                 >  SRR19973071.1338008/1‑150 (MQ=60)
                                                                                     CTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAA                                                                 >  SRR19973071.3130190/1‑150 (MQ=60)
                                                                                      TGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAG                                                                <  SRR19973071.1155054/150‑1 (MQ=60)
                                                                                      TGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAG                                                                >  SRR19973071.3933756/1‑150 (MQ=60)
                                                                                        GCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGAC                                                              <  SRR19973071.2095898/150‑1 (MQ=60)
                                                                                        GCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGAC                                                              <  SRR19973071.2095883/150‑1 (MQ=60)
                                                                                        GCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGAC                                                              <  SRR19973071.4613204/150‑1 (MQ=60)
                                                                                            AACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCA                                                          <  SRR19973071.2988227/150‑1 (MQ=60)
                                                                                              CACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAACGACGGCACC                                                        >  SRR19973071.680724/1‑150 (MQ=60)
                                                                                                 GGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTAC                                                     >  SRR19973071.1608539/1‑150 (MQ=60)
                                                                                                 GGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTAC                                                     >  SRR19973071.2021163/1‑150 (MQ=60)
                                                                                                  GCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACT                                                    <  SRR19973071.3014428/150‑1 (MQ=60)
                                                                                                  GCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACT                                                    <  SRR19973071.1105635/150‑1 (MQ=60)
                                                                                                  GCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCGGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTATCATCCAGAAAGACGGCACCTACT                                                    <  SRR19973071.1538083/150‑1 (MQ=60)
                                                                                                    AAAGGCTACGGTTGTGAAGTTTGTAAACCACCCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACTAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCG                                                  <  SRR19973071.848543/150‑1 (MQ=60)
                                                                                                     AAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGG                                                 >  SRR19973071.2440109/1‑150 (MQ=60)
                                                                                                     AAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGG                                                 >  SRR19973071.2439934/1‑150 (MQ=60)
                                                                                                          TACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATC                                            <  SRR19973071.4153820/150‑1 (MQ=60)
                                                                                                                 GTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTT                                     >  SRR19973071.802502/1‑150 (MQ=60)
                                                                                                                 GTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTT                                     >  SRR19973071.802454/1‑150 (MQ=60)
                                                                                                                 GTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTT                                     <  SRR19973071.173640/150‑1 (MQ=60)
                                                                                                                 GTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTT                                     <  SRR19973071.173759/150‑1 (MQ=60)
                                                                                                                     AGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACACCTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCC                                 >  SRR19973071.994219/1‑150 (MQ=60)
                                                                                                                     AGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCC                                 >  SRR19973071.994118/1‑150 (MQ=60)
                                                                                                                     AGTTTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCC                                 >  SRR19973071.3789842/1‑150 (MQ=60)
                                                                                                                        TTGTAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGG                              <  SRR19973071.2150896/150‑1 (MQ=60)
                                                                                                                           TAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGG                           >  SRR19973071.3442553/1‑150 (MQ=60)
                                                                                                                           TAAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGG                           <  SRR19973071.4455129/150‑1 (MQ=60)
                                                                                                                            AAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGT                          >  SRR19973071.2114139/1‑150 (MQ=60)
                                                                                                                            AAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGT                          >  SRR19973071.4521476/1‑150 (MQ=60)
                                                                                                                            AAACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGT                          >  SRR19973071.761560/1‑150 (MQ=60)
                                                                                                                             AACCAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTG                         <  SRR19973071.931677/150‑1 (MQ=60)
                                                                                                                                CAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAA                      >  SRR19973071.3954880/1‑150 (MQ=60)
                                                                                                                                CAACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAA                      >  SRR19973071.3954884/1‑150 (MQ=60)
                                                                                                                                  ACCGTCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATC                    <  SRR19973071.3319603/150‑1 (MQ=60)
                                                                                                                                      TCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCC                <  SRR19973071.3061988/150‑1 (MQ=60)
                                                                                                                                      TCGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCC                <  SRR19973071.662780/150‑1 (MQ=60)
                                                                                                                                       CGGTTCGCTGCTGCCCTCCTGCTGGAACTAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACGTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCC               <  SRR19973071.2477821/150‑1 (MQ=60)
                                                                                                                                       CGGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCC               <  SRR19973071.1618495/150‑1 (MQ=60)
                                                                                                                                        GGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCG              >  SRR19973071.1575398/1‑150 (MQ=60)
                                                                                                                                        GGTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCG              >  SRR19973071.3886678/1‑150 (MQ=60)
                                                                                                                                         GTTCGCTGCTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACCACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGG             >  SRR19973071.709100/1‑150 (MQ=60)
                                                                                                                                                 CTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGAAGGGCTG     >  SRR19973071.3112056/1‑150 (MQ=60)
                                                                                                                                                 CTGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGAAGGGCTG     >  SRR19973071.4561428/1‑150 (MQ=60)
                                                                                                                                                  TGCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGAAGGGCTGA    >  SRR19973071.4716578/1‑150 (MQ=60)
                                                                                                                                                   GCCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGAAGGGCTGAT   <  SRR19973071.1807789/150‑1 (MQ=60)
                                                                                                                                                    CCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGACGGGCTGATG  >  SRR19973071.1935728/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
CAACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACCAACCGTCGGTTCGCTGCTGGCCTCCTGCTGGAACGAATACATTCTGAAGCCGGAACATACTCCGCTGCAGGATTCTAACGACAACTTCCTCGCTAACATCCAGAAAGACGGCACCTACTCGGTGATCCCGCGTTCTCCGGGCGGTGAAATCACCCCGGAAGGGCTGATG  >  CP009273/3488818‑3489115

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑