Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F78 I0 R1 626 241.3 10389380 77.7% 8072548 149.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,405,946 T→G 100% Q43P (CAG→CCG)  yfbU ← UPF0304 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,405,9460TG95.8% 798.0 / 14.0 223Q43P (CAG→CCG) yfbUUPF0304 family protein
Reads supporting (aligned to +/- strand):  ref base T (5/5);  new base G (98/115);  total (103/120)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 223 total aligned reads displayed.

AAGGTGACACGACGTTCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACT  >  CP009273/2405799‑2406092
                                                                                                                                                   |                                                                                                                                                  
  ggTGACACGACGTTCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTaa                                                                                                                                                >  2:270219/1‑150 (MQ=255)
   gTGACACGACGTTCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAAt                                                                                                                                               <  1:4006015/150‑1 (MQ=255)
   gTGACACGACGTTCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAAt                                                                                                                                               <  1:1540248/150‑1 (MQ=255)
          gacgGTCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAAc                                                                                                                                        <  1:1777840/150‑1 (MQ=255)
             gTTCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCAc                                                                                                                                     <  1:3999620/150‑1 (MQ=255)
             gTTCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCAc                                                                                                                                     <  2:4266916/150‑1 (MQ=255)
                 aTCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATATCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGcac                                                                                                                                 >  2:1258981/1‑148 (MQ=255)
                   cGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCaa                                                                                                                               >  1:4695665/1‑150 (MQ=255)
                   cGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCaa                                                                                                                               >  2:878293/1‑150 (MQ=255)
                        gATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAAtt                                                                                                                          <  1:2986508/150‑1 (MQ=255)
                         aTTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTg                                                                                                                         <  2:2465758/150‑1 (MQ=255)
                           tGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGtt                                                                                                                       <  2:1948424/150‑1 (MQ=255)
                            gCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGttt                                                                                                                      <  2:1524839/150‑1 (MQ=255)
                              tGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGc                                                                                                                    >  2:3857117/1‑150 (MQ=255)
                                 tCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAgg                                                                                                                 <  1:205147/150‑1 (MQ=255)
                                 tCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAgg                                                                                                                 >  2:2831977/1‑150 (MQ=255)
                                    tGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGa                                                                                                              <  2:2382620/150‑1 (MQ=255)
                                    tGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGa                                                                                                              >  1:717855/1‑150 (MQ=255)
                                    tGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAAGAATTGTTTGCAGGCGa                                                                                                              >  1:3198185/1‑150 (MQ=255)
                                     gCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGAc                                                                                                             <  1:249296/150‑1 (MQ=255)
                                        aaTTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGt                                                                                                          >  2:4433616/1‑150 (MQ=255)
                                        aaTTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGt                                                                                                          <  2:1640827/150‑1 (MQ=255)
                                          ttAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTaa                                                                                                        <  1:2478185/150‑1 (MQ=255)
                                           tAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAAc                                                                                                       >  1:3891401/1‑150 (MQ=255)
                                               ccAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTc                                                                                                   >  2:1211018/1‑150 (MQ=255)
                                                 aGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAg                                                                                                 <  1:2278345/150‑1 (MQ=255)
                                                 aGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAg                                                                                                 >  2:790108/1‑150 (MQ=255)
                                                  ggAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGc                                                                                                <  2:4674327/150‑1 (MQ=255)
                                                      aCATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCAttt                                                                                            <  2:236262/150‑1 (MQ=255)
                                                      aCATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCAttt                                                                                            <  2:236742/150‑1 (MQ=255)
                                                      aCATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCAttt                                                                                            <  1:2354627/150‑1 (MQ=255)
                                                       cATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTg                                                                                           <  1:1223961/150‑1 (MQ=255)
                                                        aTGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGc                                                                                          <  1:3305967/150‑1 (MQ=255)
                                                        aTGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGc                                                                                          >  1:4023376/1‑150 (MQ=255)
                                                         tGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGcc                                                                                         >  2:5058975/1‑150 (MQ=255)
                                                          gCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCg                                                                                        <  2:1793630/150‑1 (MQ=255)
                                                            aaCGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGa                                                                                      <  1:1681507/150‑1 (MQ=255)
                                                              cGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATc                                                                                    <  1:3653510/150‑1 (MQ=255)
                                                              cGCATGATACATATCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATc                                                                                    <  1:3008580/150‑1 (MQ=255)
                                                                 aTGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACTCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAg                                                                                 >  2:887878/1‑150 (MQ=255)
                                                                 aTGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCAGTTGCCGGATCGAg                                                                                 >  2:1063060/1‑150 (MQ=255)
                                                                   gATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCa                                                                               <  2:1336431/150‑1 (MQ=255)
                                                                   gATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCa                                                                               <  2:356379/150‑1 (MQ=255)
                                                                   gATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCa                                                                               >  1:142378/1‑150 (MQ=255)
                                                                     tACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATa                                                                             >  2:4146475/1‑150 (MQ=255)
                                                                       cATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGt                                                                           >  2:1714136/1‑150 (MQ=255)
                                                                            ccATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGtcatca                                                                      <  2:470347/150‑1 (MQ=255)
                                                                             cATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTcatcat                                                                     <  2:3757170/150‑1 (MQ=255)
                                                                              aTAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCatcatc                                                                    <  1:1471047/150‑1 (MQ=255)
                                                                               tAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCt                                                                   <  2:1981673/150‑1 (MQ=255)
                                                                               tAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCt                                                                   <  1:1992017/150‑1 (MQ=255)
                                                                               tAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCt                                                                   <  1:5094707/150‑1 (MQ=255)
                                                                                aaTGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCtt                                                                  <  2:2310501/150‑1 (MQ=255)
                                                                                 aTGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTg                                                                 <  1:345864/150‑1 (MQ=255)
                                                                                  tGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGt                                                                >  2:316334/1‑150 (MQ=255)
                                                                                    tCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTAc                                                              <  1:4751787/150‑1 (MQ=255)
                                                                                      gatgatAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTg                                                            <  1:2742936/150‑1 (MQ=255)
                                                                                      gatgatAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTg                                                            <  1:3405830/150‑1 (MQ=255)
                                                                                      gatgatAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTg                                                            >  2:3822230/1‑150 (MQ=255)
                                                                                      gatgatAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTg                                                            >  1:4498392/1‑150 (MQ=255)
                                                                                      gatgatAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAAGTCACGCATCTGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTg                                                            <  1:1387013/150‑1 (MQ=255)
                                                                                        tgatAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGAt                                                          >  1:1469361/1‑150 (MQ=255)
                                                                                        tgatAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGAt                                                          <  1:1048202/150‑1 (MQ=255)
                                                                                        tgatAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGAt                                                          >  1:4277641/1‑150 (MQ=255)
                                                                                         gatAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGAtt                                                         <  2:1765243/150‑1 (MQ=255)
                                                                                             gTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGAc                                                     <  1:387103/150‑1 (MQ=255)
                                                                                                cGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACaaa                                                  <  1:4597955/150‑1 (MQ=255)
                                                                                                cGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACaaa                                                  >  2:3237788/1‑150 (MQ=255)
                                                                                                  gCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAAt                                                <  2:4606308/150‑1 (MQ=255)
                                                                                                       tttCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGAc                                           <  1:4800485/150‑1 (MQ=255)
                                                                                                        ttcttcTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACg                                          >  1:2805427/1‑150 (MQ=255)
                                                                                                        ttcttcTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACg                                          <  1:2171680/150‑1 (MQ=255)
                                                                                                             cttTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTgcg                                     <  2:1764954/150‑1 (MQ=255)
                                                                                                             cttTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGAAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTgcg                                     <  2:1826503/150‑1 (MQ=255)
                                                                                                                 cAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGtt                                 >  2:301695/1‑150 (MQ=255)
                                                                                                                 cAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGtt                                 >  1:3634411/1‑150 (MQ=255)
                                                                                                                  aGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTg                                <  1:4988206/150‑1 (MQ=255)
                                                                                                                  aGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAAGTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTg                                <  2:4940232/150‑1 (MQ=255)
                                                                                                                     tCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTc                             <  1:4150910/150‑1 (MQ=255)
                                                                                                                      cGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCa                            >  2:4663849/1‑150 (MQ=255)
                                                                                                                        ccAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCAtt                          >  2:767183/1‑150 (MQ=255)
                                                                                                                         cAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCAttt                         <  1:857093/150‑1 (MQ=255)
                                                                                                                         cAAACTCGCGATCCAAGTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCAttt                         <  2:3983407/150‑1 (MQ=255)
                                                                                                                          aaaCTCGCGATCCAATTCACGCATCGGTAATCCGTAACCNCGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTc                        >  1:4791246/1‑150 (MQ=255)
                                                                                                                              tCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCAtt                    >  1:730030/1‑150 (MQ=255)
                                                                                                                               cgcgATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCAttg                   >  2:1291921/1‑150 (MQ=255)
                                                                                                                               cgcgATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCAttg                   <  1:25330/150‑1 (MQ=255)
                                                                                                                               cgcgATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCAttg                   >  2:605737/1‑150 (MQ=255)
                                                                                                                                gcgATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCAttgt                  >  2:5111073/1‑150 (MQ=255)
                                                                                                                                  gATCCAATTCACGCATCTGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCAttgttg                <  1:2918939/150‑1 (MQ=255)
                                                                                                                                   aTCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGa               >  2:2661487/1‑150 (MQ=255)
                                                                                                                                    tCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGan              <  1:2955739/150‑2 (MQ=255)
                                                                                                                                    tCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGAc              >  2:4282410/1‑150 (MQ=255)
                                                                                                                                    tCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGAc              >  1:2018917/1‑150 (MQ=255)
                                                                                                                                      cAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTc            >  1:3291431/1‑150 (MQ=255)
                                                                                                                                         ttCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTg         <  2:4695665/150‑1 (MQ=255)
                                                                                                                                             cGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATc     <  1:2659579/150‑1 (MQ=255)
                                                                                                                                             cGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATc     <  2:469986/150‑1 (MQ=255)
                                                                                                                                               cATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCAc   <  2:1603738/150‑1 (MQ=255)
                                                                                                                                                aTCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCAct  >  1:3419792/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AAGGTGACACGACGTTCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACT  >  CP009273/2405799‑2406092

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGAAGGTGACACGACGTTCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTA  >  CP009273/2405797‑2406095
                                                                                                                                                     |                                                                                                                                                     
GGAAGGTGACACGACGTTCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCG                                                                                                                                                       >  SRR19973083.906880/1‑150 (MQ=60)
GGAAGGTGACACGACGTTCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCG                                                                                                                                                       >  SRR19973083.4419655/1‑150 (MQ=60)
GGAAGGTGACACGACGTTCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCG                                                                                                                                                       >  SRR19973083.906946/1‑150 (MQ=60)
     GTGACACGACGTTCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAAT                                                                                                                                                  <  SRR19973083.4023836/150‑1 (MQ=60)
     GTGACACGACGTTCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAAT                                                                                                                                                  <  SRR19973083.1547999/150‑1 (MQ=60)
        ACACGACGTTCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCG                                                                                                                                               >  SRR19973083.5037253/1‑150 (MQ=60)
         CACGACGTTCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGT                                                                                                                                              >  SRR19973083.15208/1‑150 (MQ=60)
            GACGGTCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAAC                                                                                                                                           <  SRR19973083.4387473/150‑1 (MQ=60)
            GACGGTCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAAC                                                                                                                                           <  SRR19973083.1786693/150‑1 (MQ=60)
               GTTCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCAC                                                                                                                                        <  SRR19973083.4017371/150‑1 (MQ=60)
               GTTCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCAC                                                                                                                                        <  SRR19973083.4017409/150‑1 (MQ=60)
               GTTCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGACGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCAC                                                                                                                                        <  SRR19973083.4017415/150‑1 (MQ=60)
                TTCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACG                                                                                                                                       <  SRR19973083.1913364/150‑1 (MQ=60)
                 TCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGC                                                                                                                                      >  SRR19973083.2322344/1‑150 (MQ=60)
                     CGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAA                                                                                                                                  >  SRR19973083.4715866/1‑150 (MQ=60)
                     CGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAA                                                                                                                                  >  SRR19973083.4715799/1‑150 (MQ=60)
                        TGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAA                                                                                                                               >  SRR19973083.3220353/1‑150 (MQ=60)
                         GGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAAT                                                                                                                              >  SRR19973083.560212/1‑150 (MQ=60)
                          GATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATT                                                                                                                             <  SRR19973083.3000975/150‑1 (MQ=60)
                          GATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATT                                                                                                                             <  SRR19973083.3001230/150‑1 (MQ=60)
                                TGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTAAGCTCTCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGC                                                                                                                       >  SRR19973083.1477775/1‑150 (MQ=60)
                                   TCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGG                                                                                                                    <  SRR19973083.206233/150‑1 (MQ=60)
                                   TCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGG                                                                                                                    <  SRR19973083.206286/150‑1 (MQ=60)
                                      TGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGA                                                                                                                 >  SRR19973083.3118341/1‑150 (MQ=60)
                                      TGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGA                                                                                                                 >  SRR19973083.721413/1‑150 (MQ=60)
                                      TGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGA                                                                                                                 >  SRR19973083.721420/1‑150 (MQ=60)
                                      TGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAAGAATTGTTTGCAGGCGA                                                                                                                 >  SRR19973083.3213386/1‑150 (MQ=60)
                                       GCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGAN                                                                                                                <  SRR19973083.250525/150‑1 (MQ=60)
                                       GCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGAC                                                                                                                <  SRR19973083.250635/150‑1 (MQ=60)
                                           ATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTA                                                                                                            <  SRR19973083.4880954/150‑1 (MQ=60)
                                            TTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAA                                                                                                           <  SRR19973083.2490856/150‑1 (MQ=60)
                                             TAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAAC                                                                                                          >  SRR19973083.3908748/1‑150 (MQ=60)
                                             TAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCGCAATAATTGTTTGCAGGCGACGGGAAC                                                                                                          >  SRR19973083.3908581/1‑150 (MQ=60)
                                                 CCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTC                                                                                                      <  SRR19973083.4797481/150‑1 (MQ=60)
                                                   AGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAG                                                                                                    <  SRR19973083.2290022/150‑1 (MQ=60)
                                                   AGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAG                                                                                                    <  SRR19973083.2290001/150‑1 (MQ=60)
                                                      TAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCAT                                                                                                 >  SRR19973083.3677647/1‑150 (MQ=60)
                                                        ACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTT                                                                                               <  SRR19973083.2366755/150‑1 (MQ=60)
                                                         CATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTG                                                                                              <  SRR19973083.1229978/150‑1 (MQ=60)
                                                          ATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGC                                                                                             <  SRR19973083.3321495/150‑1 (MQ=60)
                                                          ATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGC                                                                                             >  SRR19973083.4041260/1‑150 (MQ=60)
                                                          ATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGC                                                                                             >  SRR19973083.4041258/1‑150 (MQ=60)
                                                              AACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGA                                                                                         <  SRR19973083.1689818/150‑1 (MQ=60)
                                                              AACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGGAACGTTCAGCATTTGCCGGA                                                                                         >  SRR19973083.907881/1‑150 (MQ=60)
                                                                CGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATC                                                                                       <  SRR19973083.3670216/150‑1 (MQ=60)
                                                                CGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATC                                                                                       <  SRR19973083.2953866/150‑1 (MQ=60)
                                                                CGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATC                                                                                       <  SRR19973083.2953921/150‑1 (MQ=60)
                                                                CGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATC                                                                                       <  SRR19973083.1238169/150‑1 (MQ=60)
                                                                CGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATC                                                                                       <  SRR19973083.3670186/150‑1 (MQ=60)
                                                                CGCATGATACATATCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATC                                                                                       <  SRR19973083.3023099/150‑1 (MQ=60)
                                                                     GATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCA                                                                                  >  SRR19973083.4656384/1‑150 (MQ=60)
                                                                     GATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCA                                                                                  >  SRR19973083.143250/1‑150 (MQ=60)
                                                                     GATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCA                                                                                  >  SRR19973083.143059/1‑150 (MQ=60)
                                                                         CATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGT                                                                              >  SRR19973083.1783666/1‑150 (MQ=60)
                                                                             TCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATC                                                                          >  SRR19973083.2453346/1‑150 (MQ=60)
                                                                                ATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATC                                                                       <  SRR19973083.1478455/150‑1 (MQ=60)
                                                                                 TAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCT                                                                      <  SRR19973083.2002197/150‑1 (MQ=60)
                                                                                 TAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCT                                                                      <  SRR19973083.4601689/150‑1 (MQ=60)
                                                                                 TAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCT                                                                      <  SRR19973083.5116461/150‑1 (MQ=60)
                                                                                   ATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTG                                                                    >  SRR19973083.2974647/1‑150 (MQ=60)
                                                                                   ATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTG                                                                    <  SRR19973083.347525/150‑1 (MQ=60)
                                                                                      TCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTAC                                                                 <  SRR19973083.1930771/150‑1 (MQ=60)
                                                                                      TCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTAC                                                                 <  SRR19973083.4772055/150‑1 (MQ=60)
                                                                                      TCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTAC                                                                 <  SRR19973083.4772062/150‑1 (MQ=60)
                                                                                        GATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTG                                                               <  SRR19973083.2756697/150‑1 (MQ=60)
                                                                                        GATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTG                                                               >  SRR19973083.3463501/1‑150 (MQ=60)
                                                                                        GATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTG                                                               >  SRR19973083.4517921/1‑150 (MQ=60)
                                                                                        GATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTG                                                               <  SRR19973083.3421833/150‑1 (MQ=60)
                                                                                        GATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTG                                                               <  SRR19973083.3421563/150‑1 (MQ=60)
                                                                                        GATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTG                                                               <  SRR19973083.2756611/150‑1 (MQ=60)
                                                                                        GATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAAGTCACGCATCTGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTG                                                               <  SRR19973083.1393935/150‑1 (MQ=60)
                                                                                          TGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGAT                                                             >  SRR19973083.4296293/1‑150 (MQ=60)
                                                                                          TGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGAT                                                             >  SRR19973083.1477100/1‑150 (MQ=60)
                                                                                          TGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGAT                                                             >  SRR19973083.1476763/1‑150 (MQ=60)
                                                                                          TGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGAT                                                             <  SRR19973083.1053476/150‑1 (MQ=60)
                                                                                             TAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAG                                                          >  SRR19973083.795988/1‑150 (MQ=60)
                                                                                               GTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGAC                                                        <  SRR19973083.388888/150‑1 (MQ=60)
                                                                                                  CGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAA                                                     <  SRR19973083.4617811/150‑1 (MQ=60)
                                                                                                      AGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAA                                                 <  SRR19973083.3319473/150‑1 (MQ=60)
                                                                                                      AGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAA                                                 <  SRR19973083.3987105/150‑1 (MQ=60)
                                                                                                         TTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGAC                                              <  SRR19973083.4821016/150‑1 (MQ=60)
                                                                                                          TTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACG                                             >  SRR19973083.2819345/1‑150 (MQ=60)
                                                                                                          TTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACG                                             <  SRR19973083.2182587/150‑1 (MQ=60)
                                                                                                          TTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACG                                             <  SRR19973083.2182564/150‑1 (MQ=60)
                                                                                                          TTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACG                                             >  SRR19973083.4162961/1‑150 (MQ=60)
                                                                                                               CTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCG                                        >  SRR19973083.362239/1‑150 (MQ=60)
                                                                                                                TTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGC                                       <  SRR19973083.1116647/150‑1 (MQ=60)
                                                                                                                   CAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTT                                    >  SRR19973083.3651043/1‑150 (MQ=60)
                                                                                                                   CAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTT                                    >  SRR19973083.3651037/1‑150 (MQ=60)
                                                                                                                    AGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTG                                   <  SRR19973083.5009624/150‑1 (MQ=60)
                                                                                                                       TCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTC                                <  SRR19973083.4169234/150‑1 (MQ=60)
                                                                                                                        CGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCA                               >  SRR19973083.1624375/1‑150 (MQ=60)
                                                                                                                        CGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCA                               >  SRR19973083.4224119/1‑150 (MQ=60)
                                                                                                                          CCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATT                             <  SRR19973083.2712546/150‑1 (MQ=60)
                                                                                                                           CAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTT                            <  SRR19973083.861115/150‑1 (MQ=60)
                                                                                                                            AAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCNCGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTC                           >  SRR19973083.4811719/1‑150 (MQ=60)
                                                                                                                            AAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCNCGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGGTGGTCATTTC                           >  SRR19973083.4811720/1‑150 (MQ=60)
                                                                                                                                TCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATT                       >  SRR19973083.733629/1‑150 (MQ=60)
                                                                                                                                 CGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTG                      <  SRR19973083.25543/150‑1 (MQ=60)
                                                                                                                                 CGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTG                      <  SRR19973083.25724/150‑1 (MQ=60)
                                                                                                                                   CGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTT                    >  SRR19973083.2823906/1‑150 (MQ=60)
                                                                                                                                    GATCCAATTCACGCATCTGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTG                   <  SRR19973083.2933219/150‑1 (MQ=60)
                                                                                                                                     ATCCAATTCACGCATCTGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGA                  >  SRR19973083.3333411/1‑150 (MQ=60)
                                                                                                                                     ATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGA                  >  SRR19973083.5130867/1‑150 (MQ=60)
                                                                                                                                      TCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGAN                 <  SRR19973083.2970122/150‑1 (MQ=60)
                                                                                                                                      TCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGAC                 >  SRR19973083.2029155/1‑150 (MQ=60)
                                                                                                                                      TCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGAC                 >  SRR19973083.2029228/1‑150 (MQ=60)
                                                                                                                                      TCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGAC                 <  SRR19973083.2066101/150‑1 (MQ=60)
                                                                                                                                        CAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTC               >  SRR19973083.3306953/1‑150 (MQ=60)
                                                                                                                                        CAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTC               >  SRR19973083.3306918/1‑150 (MQ=60)
                                                                                                                                          ATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCT             >  SRR19973083.4442766/1‑150 (MQ=60)
                                                                                                                                          ATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCT             >  SRR19973083.2528705/1‑150 (MQ=60)
                                                                                                                                             CACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTA          >  SRR19973083.1440730/1‑150 (MQ=60)
                                                                                                                                               CGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATC        <  SRR19973083.2672687/150‑1 (MQ=60)
                                                                                                                                               CGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATC        <  SRR19973083.2672927/150‑1 (MQ=60)
                                                                                                                                                  ATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACT     >  SRR19973083.3435555/1‑150 (MQ=60)
                                                                                                                                                   TCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTC    <  SRR19973083.278995/150‑1 (MQ=60)
                                                                                                                                                   TCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTC    <  SRR19973083.2905210/150‑1 (MQ=60)
                                                                                                                                                     GGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTA  >  SRR19973083.786714/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
GGAAGGTGACACGACGTTCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTA  >  CP009273/2405797‑2406095

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑