Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F488 I0 R1 1275 300.1 10328444 91.0% 9398884 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,405,946 T→G 100% Q43P (CAG→CCG)  yfbU ← UPF0304 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,405,9460TG98.9% 1073.1 / ‑2.4 277Q43P (CAG→CCG) yfbUUPF0304 family protein
Reads supporting (aligned to +/- strand):  ref base T (2/1);  new base G (138/136);  total (140/137)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 277 total aligned reads displayed.

AAGGTGACACGACGTTCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACT  >  CP009273/2405799‑2406092
                                                                                                                                                   |                                                                                                                                                  
aaGGTGACACGACGTTCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGt                                                                                                                                                  <  2:1613823/150‑1 (MQ=255)
    tGACACGACGTTCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAAAGTCGATGATAGTGCGGAAGGTTTCTTCTATCAGCTCGCCAAACTCGCGATCCAAAACACGCCTCGGTACTc                                                                                                                                              >  1:5084464/1‑150 (MQ=255)
       cacGACGTTCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGt                                                                                                                                           >  2:4252753/1‑150 (MQ=255)
        acgacgTTCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTa                                                                                                                                          <  2:2832631/150‑1 (MQ=255)
           acgTTCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAAcc                                                                                                                                       <  2:4887279/150‑1 (MQ=255)
               tCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGc                                                                                                                                   >  2:2426272/1‑150 (MQ=255)
                cATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCt                                                                                                                                  >  1:4854815/1‑150 (MQ=255)
                  tCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCa                                                                                                                                <  1:1664400/150‑1 (MQ=255)
                  tCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCa                                                                                                                                <  1:4235859/150‑1 (MQ=255)
                  tCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCa                                                                                                                                <  1:120571/150‑1 (MQ=255)
                        gATTGCTGGTCCTGCAAATTAGACCAGGAAACATGAAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCTGTAACCACGCTCAATAAtt                                                                                                                          <  1:1747679/150‑1 (MQ=255)
                         aTTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTg                                                                                                                         <  1:2763505/150‑1 (MQ=255)
                          ttGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGt                                                                                                                        >  1:4509760/1‑150 (MQ=255)
                           tGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGtt                                                                                                                       >  2:720762/1‑150 (MQ=255)
                              tGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGc                                                                                                                    >  2:4335924/1‑150 (MQ=255)
                              tGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGc                                                                                                                    <  2:787960/150‑1 (MQ=255)
                               ggTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCa                                                                                                                   >  2:2484544/1‑150 (MQ=255)
                               ggTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCa                                                                                                                   <  2:1034676/150‑1 (MQ=255)
                                  ccTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGc                                                                                                                <  1:3797410/150‑1 (MQ=255)
                                   cTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCg                                                                                                               <  2:2671213/150‑1 (MQ=255)
                                     gCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGAc                                                                                                             <  2:4668457/150‑1 (MQ=255)
                                       aaaTTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACgg                                                                                                           >  2:4477145/1‑150 (MQ=255)
                                        aaTTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGt                                                                                                          >  2:1767011/1‑150 (MQ=255)
                                          ttAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTaa                                                                                                        <  1:5140075/150‑1 (MQ=255)
                                           tAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAAc                                                                                                       <  2:3837291/150‑1 (MQ=255)
                                           tAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAAc                                                                                                       >  1:2321773/1‑150 (MQ=255)
                                           tAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAAc                                                                                                       <  2:1257942/150‑1 (MQ=255)
                                           tAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGGAAc                                                                                                       >  2:4300018/1‑150 (MQ=255)
                                            aGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACg                                                                                                      >  2:4186083/1‑150 (MQ=255)
                                               ccAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTc                                                                                                   >  2:2096715/1‑150 (MQ=255)
                                               ccAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTc                                                                                                   <  2:1528177/150‑1 (MQ=255)
                                                cAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCa                                                                                                  >  2:2335300/1‑150 (MQ=255)
                                                cAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCa                                                                                                  <  1:3919238/150‑1 (MQ=255)
                                                 aGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAg                                                                                                 >  1:2262812/1‑150 (MQ=255)
                                                     aaCATGCAACGCATGATACATCTCCATAATGTTGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCAtt                                                                                             >  2:930301/1‑150 (MQ=255)
                                                       cATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTg                                                                                           >  2:3602204/1‑150 (MQ=255)
                                                       cATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTg                                                                                           >  2:1461070/1‑150 (MQ=255)
                                                           cAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCgg                                                                                       >  2:552234/1‑150 (MQ=255)
                                                              cGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATc                                                                                    >  1:2955102/1‑150 (MQ=255)
                                                               gCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCg                                                                                   <  1:1576592/150‑1 (MQ=255)
                                                                cATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGa                                                                                  <  1:3319606/150‑1 (MQ=255)
                                                                cATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGa                                                                                  >  2:3813395/1‑150 (MQ=255)
                                                                cATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGa                                                                                  >  2:1653785/1‑150 (MQ=255)
                                                                cATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGa                                                                                  <  1:4410403/150‑1 (MQ=255)
                                                                 aTGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAg                                                                                 <  1:371087/150‑1 (MQ=255)
                                                                  tGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGc                                                                                >  1:2707022/1‑150 (MQ=255)
                                                                     tACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATa                                                                             <  2:3169303/150‑1 (MQ=255)
                                                                      aCATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAg                                                                            <  1:777500/150‑1 (MQ=255)
                                                                      aCATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAg                                                                            <  2:4757662/150‑1 (MQ=255)
                                                                         tctcCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGtca                                                                         <  1:83615/150‑1 (MQ=255)
                                                                          ctcCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGtcat                                                                        <  1:4802931/150‑1 (MQ=255)
                                                                          ctcCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGtcat                                                                        <  2:5039336/150‑1 (MQ=255)
                                                                           tcCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGtcatc                                                                       >  1:180730/1‑150 (MQ=255)
                                                                             cATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTcatcat                                                                     <  2:1860803/150‑1 (MQ=255)
                                                                              aTAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCatcatc                                                                    >  1:3623997/1‑150 (MQ=255)
                                                                              aTAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCatcatc                                                                    <  2:2695435/150‑1 (MQ=255)
                                                                               tAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCt                                                                   <  1:4252812/150‑1 (MQ=255)
                                                                                aaTGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCtt                                                                  >  1:4757767/1‑150 (MQ=255)
                                                                                aaTGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCtt                                                                  <  2:3321923/150‑1 (MQ=255)
                                                                                aaTGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCtt                                                                  >  1:923306/1‑150 (MQ=255)
                                                                                aaTGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCtt                                                                  <  1:1469080/150‑1 (MQ=255)
                                                                                aaTGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGACAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCATGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCtt                                                                  <  2:30800/150‑1 (MQ=255)
                                                                                 aTGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTg                                                                 >  2:4774719/1‑150 (MQ=255)
                                                                                   gTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTAGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTa                                                               >  1:2425529/1‑150 (MQ=255)
                                                                                    tCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCATCATTTGCCGGATCGAGCATAGTCATCATCTTTTAc                                                              <  1:4032330/150‑1 (MQ=255)
                                                                                     cGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACt                                                             >  2:3031652/1‑150 (MQ=255)
                                                                                     cGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACt                                                             >  2:1891550/1‑150 (MQ=255)
                                                                                        tgatAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGAt                                                          >  2:1828329/1‑150 (MQ=255)
                                                                                         gatAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGAtt                                                         <  1:2154077/150‑1 (MQ=255)
                                                                                          atAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTa                                                        >  2:3105387/1‑150 (MQ=255)
                                                                                               gCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACaa                                                   >  1:3855159/1‑150 (MQ=255)
                                                                                               gCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACaa                                                   <  1:3971802/150‑1 (MQ=255)
                                                                                                   cAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATa                                               >  2:1152768/1‑150 (MQ=255)
                                                                                                    aGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATaa                                              <  2:3907533/150‑1 (MQ=255)
                                                                                                        ttcttcTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACg                                          >  2:4572765/1‑150 (MQ=255)
                                                                                                         tcttctTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGt                                         >  1:1007746/1‑150 (MQ=255)
                                                                                                         tcttctTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTGGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGt                                         <  2:2307108/150‑1 (MQ=255)
                                                                                                           ttcttTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTg                                       <  1:3104898/150‑1 (MQ=255)
                                                                                                             cttTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTgcg                                     <  1:3496900/150‑1 (MQ=255)
                                                                                                             cttTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTgcg                                     >  2:3430911/1‑150 (MQ=255)
                                                                                                             cttTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTgcg                                     <  2:1421592/150‑1 (MQ=255)
                                                                                                                tCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAAGTGTTTGCAGGCGACGGTAACGTACAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGt                                  >  1:1785877/1‑150 (MQ=255)
                                                                                                                   gCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTgg                               <  2:215019/150‑1 (MQ=255)
                                                                                                                    cTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGt                              <  2:3737983/150‑1 (MQ=255)
                                                                                                                      cGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCa                            <  1:4687591/150‑1 (MQ=255)
                                                                                                                      cGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCa                            >  2:1291496/1‑150 (MQ=255)
                                                                                                                          aaaCTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTc                        <  1:2972095/150‑1 (MQ=255)
                                                                                                                            aCTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCa                      >  2:3980164/1‑150 (MQ=255)
                                                                                                                               cgcgATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCAttg                   >  1:1126766/1‑150 (MQ=255)
                                                                                                                                gcgATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCAttgt                  >  1:1082956/1‑150 (MQ=255)
                                                                                                                                 cgATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCAttgtt                 <  1:680208/150‑1 (MQ=255)
                                                                                                                                 cgATACAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGTTCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCAttgtt                 <  1:5131528/150‑1 (MQ=255)
                                                                                                                                    tCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGAc              <  1:2071969/150‑1 (MQ=255)
                                                                                                                                        aTTCACGCATCGGTAATCAGTAACAACGCTCAATAATTGTTTGCAGGAGACGGTAACTTTCAGCATTTGCCTGATCGAGCATAGTCATCATCTTGTAATGATTAGACAAAATAAGACTTTGCGCGTTGGTCATTTCCATTGTTGACTcat          >  2:4424809/1‑148 (MQ=255)
                                                                                                                                          tCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGt        <  2:4069470/150‑1 (MQ=255)
                                                                                                                                             cGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGATGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATc     >  2:4499998/1‑150 (MQ=255)
                                                                                                                                             cGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATc     >  2:2207110/1‑150 (MQ=255)
                                                                                                                                              gCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCa    >  2:3942634/1‑150 (MQ=255)
                                                                                                                                               cATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCAc   <  2:640099/150‑1 (MQ=255)
                                                                                                                                                aTCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCAct  >  1:3935756/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AAGGTGACACGACGTTCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACT  >  CP009273/2405799‑2406092

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GAAGGTGACACGACGTTCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTA  >  CP009273/2405798‑2406095
                                                                                                                                                    |                                                                                                                                                     
GAAGGTGACACGACGTTCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGG                                                                                                                                                      <  SRR19973063.4465647/150‑1 (MQ=60)
GAAGGTGACACGACGTTCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGG                                                                                                                                                      <  SRR19973063.4644643/150‑1 (MQ=60)
    GTGACACGACGTTCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAAT                                                                                                                                                  <  SRR19973063.3954284/150‑1 (MQ=60)
          CGACGTTCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAA                                                                                                                                            <  SRR19973063.1123908/150‑1 (MQ=60)
          CGACGTTCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAA                                                                                                                                            >  SRR19973063.640303/1‑150 (MQ=60)
          CGACGTTCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAA                                                                                                                                            >  SRR19973063.2978393/1‑150 (MQ=60)
                 CATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCT                                                                                                                                     >  SRR19973063.4870457/1‑150 (MQ=60)
                   TCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCA                                                                                                                                   <  SRR19973063.4249363/150‑1 (MQ=60)
                   TCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCA                                                                                                                                   <  SRR19973063.1669477/150‑1 (MQ=60)
                   TCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCA                                                                                                                                   <  SRR19973063.1669469/150‑1 (MQ=60)
                   TCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCA                                                                                                                                   <  SRR19973063.120971/150‑1 (MQ=60)
                   TCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCA                                                                                                                                   >  SRR19973063.2609292/1‑150 (MQ=60)
                      ATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCTTGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCAGTAACCAAGCTCAATA                                                                                                                                >  SRR19973063.1798635/1‑150 (MQ=60)
                      ATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCGTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATAGGTAATCCGTAACCACGCTCAATA                                                                                                                                >  SRR19973063.3064381/1‑150 (MQ=60)
                         GATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATT                                                                                                                             <  SRR19973063.1752929/150‑1 (MQ=60)
                         GATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATT                                                                                                                             <  SRR19973063.1753028/150‑1 (MQ=60)
                         GATTGCTGGTCCTGCAAATTAGACCAGGAAACATGAAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCTGTAACCACGCTCAATAATT                                                                                                                             <  SRR19973063.1753036/150‑1 (MQ=60)
                          ATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTG                                                                                                                            <  SRR19973063.2772413/150‑1 (MQ=60)
                          ATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTG                                                                                                                            <  SRR19973063.4477632/150‑1 (MQ=60)
                           TTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGT                                                                                                                           >  SRR19973063.4524274/1‑150 (MQ=60)
                           TTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGT                                                                                                                           >  SRR19973063.4524232/1‑150 (MQ=60)
                             GCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTT                                                                                                                         <  SRR19973063.2732186/150‑1 (MQ=60)
                              CTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTG                                                                                                                        >  SRR19973063.2912961/1‑150 (MQ=60)
                                GGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCA                                                                                                                      <  SRR19973063.1288858/150‑1 (MQ=60)
                                GGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCA                                                                                                                      <  SRR19973063.2513625/150‑1 (MQ=60)
                                 GTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAG                                                                                                                     <  SRR19973063.1553784/150‑1 (MQ=60)
                                   CCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGC                                                                                                                   <  SRR19973063.3809512/150‑1 (MQ=60)
                                    CTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCG                                                                                                                  <  SRR19973063.5127230/150‑1 (MQ=60)
                                       CAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACG                                                                                                               <  SRR19973063.3351560/150‑1 (MQ=60)
                                       CAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACG                                                                                                               >  SRR19973063.2151932/1‑150 (MQ=60)
                                       CAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACG                                                                                                               <  SRR19973063.758519/150‑1 (MQ=60)
                                        AAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGG                                                                                                              >  SRR19973063.3761890/1‑150 (MQ=60)
                                           TTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAA                                                                                                           <  SRR19973063.5156610/150‑1 (MQ=60)
                                           TTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAA                                                                                                           <  SRR19973063.5156595/150‑1 (MQ=60)
                                            TAGCCCAGGAAACATGCAACGCATGATACATCTCCAGAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAAC                                                                                                          <  SRR19973063.1112551/150‑1 (MQ=60)
                                            TAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAAC                                                                                                          >  SRR19973063.2329195/1‑150 (MQ=60)
                                            TAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAAC                                                                                                          >  SRR19973063.869104/1‑150 (MQ=60)
                                            TAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAAC                                                                                                          >  SRR19973063.1210360/1‑150 (MQ=60)
                                                 CAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCA                                                                                                     <  SRR19973063.3931743/150‑1 (MQ=60)
                                                  AGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAG                                                                                                    >  SRR19973063.2270020/1‑150 (MQ=60)
                                                  AGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAG                                                                                                    <  SRR19973063.3936555/150‑1 (MQ=60)
                                                      AACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATT                                                                                                <  SRR19973063.1167759/150‑1 (MQ=60)
                                                        CATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTG                                                                                              <  SRR19973063.4768521/150‑1 (MQ=60)
                                                         ATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGC                                                                                             <  SRR19973063.3329510/150‑1 (MQ=60)
                                                           GCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCG                                                                                           <  SRR19973063.4794351/150‑1 (MQ=60)
                                                           GCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCG                                                                                           >  SRR19973063.175710/1‑150 (MQ=60)
                                                             AACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGA                                                                                         >  SRR19973063.3857275/1‑150 (MQ=60)
                                                               CGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATC                                                                                       <  SRR19973063.3420968/150‑1 (MQ=60)
                                                               CGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATC                                                                                       >  SRR19973063.2964547/1‑150 (MQ=60)
                                                                GCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCG                                                                                      <  SRR19973063.1581425/150‑1 (MQ=60)
                                                                 CATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGA                                                                                     >  SRR19973063.1666188/1‑150 (MQ=60)
                                                                 CATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGA                                                                                     <  SRR19973063.3330211/150‑1 (MQ=60)
                                                                 CATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGA                                                                                     <  SRR19973063.4424546/150‑1 (MQ=60)
                                                                  ATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAG                                                                                    <  SRR19973063.372146/150‑1 (MQ=60)
                                                                   TGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGC                                                                                   >  SRR19973063.5059287/1‑150 (MQ=60)
                                                                   TGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGC                                                                                   >  SRR19973063.2715762/1‑150 (MQ=60)
                                                                   TGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGC                                                                                   >  SRR19973063.2301605/1‑150 (MQ=60)
                                                                     ATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAAGTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCAT                                                                                 <  SRR19973063.4084365/150‑1 (MQ=60)
                                                                       ACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAG                                                                               <  SRR19973063.779757/150‑1 (MQ=60)
                                                                       ACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAG                                                                               <  SRR19973063.1166157/150‑1 (MQ=60)
                                                                          TCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCA                                                                            <  SRR19973063.83836/150‑1 (MQ=60)
                                                                          TCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCA                                                                            <  SRR19973063.83905/150‑1 (MQ=60)
                                                                          TCTCCATAAGGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCA                                                                            <  SRR19973063.83601/150‑1 (MQ=60)
                                                                           CTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCAT                                                                           <  SRR19973063.3182182/150‑1 (MQ=60)
                                                                           CTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCAT                                                                           <  SRR19973063.4818287/150‑1 (MQ=60)
                                                                            TCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATC                                                                          >  SRR19973063.181290/1‑150 (MQ=60)
                                                                              CATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCAT                                                                        <  SRR19973063.5138392/150‑1 (MQ=60)
                                                                               ATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATC                                                                       >  SRR19973063.3635660/1‑150 (MQ=60)
                                                                               ATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATC                                                                       >  SRR19973063.3635629/1‑150 (MQ=60)
                                                                                TAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCT                                                                      <  SRR19973063.4266297/150‑1 (MQ=60)
                                                                                TAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCT                                                                      <  SRR19973063.4266357/150‑1 (MQ=60)
                                                                                TAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCT                                                                      >  SRR19973063.206096/1‑150 (MQ=60)
                                                                                 AATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTT                                                                     >  SRR19973063.926044/1‑150 (MQ=60)
                                                                                 AATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTT                                                                     <  SRR19973063.2034168/150‑1 (MQ=60)
                                                                                 AATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTT                                                                     <  SRR19973063.1473572/150‑1 (MQ=60)
                                                                                 AATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTT                                                                     >  SRR19973063.4772939/1‑150 (MQ=60)
                                                                                    GTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTAGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTA                                                                  >  SRR19973063.2433283/1‑150 (MQ=60)
                                                                                     TCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCATCATTTGCCGGATCGAGCATAGTCATCATCTTTTAC                                                                 <  SRR19973063.4045255/150‑1 (MQ=60)
                                                                                     TCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTAC                                                                 >  SRR19973063.3724244/1‑150 (MQ=60)
                                                                                          GATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATT                                                            <  SRR19973063.2160824/150‑1 (MQ=60)
                                                                                          GATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATT                                                            <  SRR19973063.3957088/150‑1 (MQ=60)
                                                                                          GATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATT                                                            >  SRR19973063.3072833/1‑150 (MQ=60)
                                                                                          GATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATT                                                            >  SRR19973063.482698/1‑150 (MQ=60)
                                                                                            TAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAG                                                          >  SRR19973063.641982/1‑150 (MQ=60)
                                                                                             AGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATAGGTAATCAGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGA                                                         >  SRR19973063.1080494/1‑150 (MQ=60)
                                                                                                GCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAA                                                      <  SRR19973063.3984517/150‑1 (MQ=60)
                                                                                                GCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAA                                                      >  SRR19973063.3867419/1‑150 (MQ=60)
                                                                                                   GCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAAT                                                   >  SRR19973063.2766713/1‑150 (MQ=60)
                                                                                                        TTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGAC                                              >  SRR19973063.3608097/1‑150 (MQ=60)
                                                                                                         TTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACG                                             >  SRR19973063.4340062/1‑150 (MQ=60)
                                                                                                         TTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACG                                             >  SRR19973063.1756707/1‑150 (MQ=60)
                                                                                                          TCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGT                                            >  SRR19973063.1010784/1‑150 (MQ=60)
                                                                                                            TTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTG                                          <  SRR19973063.3114888/150‑1 (MQ=60)
                                                                                                             TCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGC                                         <  SRR19973063.4832149/150‑1 (MQ=60)
                                                                                                              CTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCG                                        <  SRR19973063.3508082/150‑1 (MQ=60)
                                                                                                                 TCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAAGTGTTTGCAGGCGACGGTAACGTACAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGT                                     >  SRR19973063.1791400/1‑150 (MQ=60)
                                                                                                                       CGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCA                               <  SRR19973063.4702536/150‑1 (MQ=60)
                                                                                                                           AAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTC                           <  SRR19973063.2981595/150‑1 (MQ=60)
                                                                                                                             ACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCA                         >  SRR19973063.4965849/1‑150 (MQ=60)
                                                                                                                              CTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCAT                        >  SRR19973063.4096426/1‑150 (MQ=60)
                                                                                                                                CGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTG                      >  SRR19973063.3825580/1‑150 (MQ=60)
                                                                                                                                CGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTG                      >  SRR19973063.1130186/1‑150 (MQ=60)
                                                                                                                                 GCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGT                     >  SRR19973063.1086250/1‑150 (MQ=60)
                                                                                                                                 GCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGT                     >  SRR19973063.2412150/1‑150 (MQ=60)
                                                                                                                                  CGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTT                    <  SRR19973063.682200/150‑1 (MQ=60)
                                                                                                                                  CGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTT                    >  SRR19973063.4803489/1‑150 (MQ=60)
                                                                                                                                  CGATACAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGTTCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTT                    <  SRR19973063.5148034/150‑1 (MQ=60)
                                                                                                                                   GATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTG                   <  SRR19973063.2291032/150‑1 (MQ=60)
                                                                                                                                     TCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGAC                 <  SRR19973063.2078445/150‑1 (MQ=60)
                                                                                                                                     TCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGAC                 <  SRR19973063.2082027/150‑1 (MQ=60)
                                                                                                                                     TCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGAC                 <  SRR19973063.4434967/150‑1 (MQ=60)
                                                                                                                                     TCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGAC                 >  SRR19973063.3912650/1‑150 (MQ=60)
                                                                                                                                     TCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGAC                 >  SRR19973063.3913136/1‑150 (MQ=60)
                                                                                                                                      CCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACT                >  SRR19973063.4669482/1‑150 (MQ=60)
                                                                                                                                      CCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACT                >  SRR19973063.703813/1‑150 (MQ=60)
                                                                                                                                        AATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCC              >  SRR19973063.2904816/1‑150 (MQ=60)
                                                                                                                                        AATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCC              <  SRR19973063.2167110/150‑1 (MQ=60)
                                                                                                                                        AATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCC              <  SRR19973063.2167101/150‑1 (MQ=60)
                                                                                                                                         ATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCT             >  SRR19973063.4012021/1‑150 (MQ=60)
                                                                                                                                         ATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCT             >  SRR19973063.4011962/1‑150 (MQ=60)
                                                                                                                                              CGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATC        <  SRR19973063.368930/150‑1 (MQ=60)
                                                                                                                                                CATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTCTCAC      >  SRR19973063.4345629/1‑150 (MQ=60)
                                                                                                                                                 ATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACT     >  SRR19973063.3948331/1‑150 (MQ=60)
                                                                                                                                                    GGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTA  >  SRR19973063.3837021/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
GAAGGTGACACGACGTTCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTA  >  CP009273/2405798‑2406095

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑